Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
the distribution of folates in rabbit tissues during phenylhydrazine-induced haemolysis. 19694978549
improvement of the microbiological assay for calcium leucovorin.the calcium leucovorin assay was improved by adding thoral germicide which resuspended the cells so that an accurate turbidity reading could be obtained.19695308851
pediococci residing on plants.the pediococci residing on plants resemble the lactobacilli, but they differ from the streptococci in their limited distribution and low population level on plants. they are a subgroup within the genus pediococcus which grow freely in neutral media and require neither nacl nor co(2). they are most readily recognized by the ability to initiate growth in liquid media, acidified to ph 5.0, which contain 1.5% sodium acetate. in stained preparations the cells occur singly and in pairs, short chains, ...19695788719
diastereoisomers of formaldehyde derivatives of tetrahydrofolic acid and tetrahydroaminopterin. 19694883936
[formation of diacetyl and pentane-2,3-dione in pediococcus cerevisiae]. 19695360878
biosynthesis of mucopeptide by pediococcus cerevisiae and by a substrain requiring methicillin for growth. 19695362658
purity of amino acids. 19705424159
tetrad-forming cocci in ciders. 19705448251
plate assay methods for amino acids. 9. threonine. 19705450404
recognition of aerococcus viridans by the clinical microbiologist. 19705507757
n'-(substituted) pantothenamides, antimetabolites of pantothenic acid. 19704909169
sugar substrates for l-lysine fermentation by ustilago maydis.the extracellular production of l-lysine in media with cane sugar, blackstrap molasses, or clarified sugar-cane juice by a previously obtained mutant of ustilago maydis was studied. enzymatically inverted clarified juice (medium j-3) gave 2.9 g of lysine per liter under the following conditions: inoculum, 5%; ph 5.8; temperature, 30 c; k(la) in the fermentors, 0.41 mmoles of o(2) per liter per min; fermentation time, 72 hr. the concentrate, obtained by direct evaporation and drying of the fermen ...19705485081
transport of folinate and related compounds in pediococcus cerevisiae.the properties of folinate and 5-methyltetrahydrofolate (5-ch(3)-h(4)pteglu) transport mechanism of pediococcus cerevisiae were studied. the uptake was dependent on temperature, ph (optimum for both compounds at ph 6.0), and glucose. iodoacetate, potassium fluoride, and sodium azide inhibited the uptake. 5-ch(3)-h(4)-pteglu was apparently not metabolized but folinate was metabolized. metabolism of folinate was reduced by preincubation of cells with fluorodeoxyuridine. the transport system for fo ...19705473888
beta-alanyl thio esters. 19704909637
[nature of the malic enzyme of lactic bacteria isolated from wines]. 19704985472
urinary excretion of folic acid activity in man. 19704987973
effect of excision and benzimidazole treatment on folate content of wheat leaves and wheat leaf chloroplasts. 19704991049
synthesis of 1-amino-2-hydroxycyclopentanecarboxylic acid. 19714997124
cell wall lytic enzymes of microbial origin and their application]. 19715001159
folate antagonists as antibacterial and antiprotozoal agents. 19715002439
pteroylglutamate derivatives in pisum sativum l. biosynthesis of cotyledonary tetrahydropteroylglutamates during germination.1. the concentrations of pteroylglutamate derivatives in the cotyledons of pea (pisum sativum l.) seedlings were determined by microbiological assay by using lactobacillus casei (a.t.c.c. 7469), streptococcus faecalis (a.t.c.c. 8043) and pediococcus cerevisiae (a.t.c.c. 8081). during germination the pteroylglutamate content of the cotyledons increased rapidly from 0.2mug/g dry wt. to 4.0mug/g dry wt., the maximum values being reached approx. 120h after imbibition. 2. approx. 50% of the pteroylgl ...19715003530
[the sensitivity of microorganisms to growth factors. ii. effect of various reducing agents on the extraction and determination of folates by lactobacillus casei, streptococcus faecalis and pediococcus cerevisiae]. 19715003561
2,4-diamino-6-arylethylpteridines as streptococcus faecium growth inhibitors. 19715003787
[on the formation of volatile by-products of fermentation by lactic acid bacteria]. 19715092512
symposium on microbial changes in foods. microbiological aspects of production and spoilage of cider. 19715564383
[absorption of synthetic acid folic acid and of its natural forms in the alimentary tract]. 19715314219
nicotinamide adenine dinucleotide-dependent and nicotinamide adenine dinucleotide-independent lactate dehydrogenases in homofermentative and heterofermentative lactic acid bacteria.three homofermentative (lactobacillus plantarum b38, l. plantarum b33, pediococcus pentosaceus b30) and three heterofermentative (leuconostoc mesenteroides 39, l. oenos b70, lactobacillus brevis) lactic acid bacteria were examined for the presence or absence of nicotinamide adenine dinucleotide (nad)-dependent and nad-independent d- and l-lactate dehydrogenases. two of the six strains investigated, p. pentosaceus and l. oenos, did not exhibit an nad-independent enzyme activity capable of reducin ...19714333320
dna base composition of gram-positive cocci. 19715146835
effect of ph and oxygen tension on staphylococcal growth and enterotoxin formation in fermented sausage.commercial fermented 0sausages that contained significant numbers of viable coagulase-positive staphylococci were found to have the growth localized in the outermost areas of the sausage where oxygen tension was highest. staphylococci were found to be more acid-tolerant aerobically than anaerobically. with chemical acidulation of sausage, growth could be controlled both aerobically and anaerobically with approximately 1.5% glucono delta lactone. biological acidulation with a high inoculum of ped ...19724631103
difference between manganese ion requirements of pediococci and enterococci.for maximal turbidity and dissimilatory activity, three strains of pediococci required the addition of mn(2+) to rogosa basal medium. twenty-two strains of enterococci proved indifferent to this supplement.19725079080
[decomposition of tartrate by lactobacilli]. 19724337553
intestinal conversion of folinic acid to 5-methyltetrahydrofolate in man. 19724621839
the formation of rhizopterin and formylated folate polyglutamates during the growth of staphylococcus epidermidis. 19724624549
localization and interconversion of tetrahydropteroylglutamates in isolated pea mitochondria.1. mitochondria were extracted from 4-day-old pea cotyledons and purified on a sucrose density gradient. 2. microbiological assay of the purified mitochondrial fraction with lactobacillus casei (a.t.c.c. 7469), streptococcus faecalis (a.t.c.c. 8043) and pediococcus cerevisiae (a.t.c.c. 8081) revealed a discrete pool of conjugated and unconjugated derivatives of tetrahydropteroylglutamic acid. 3. solubilization and chromatographic studies of the mitochondrial fraction demonstrated the presence of ...19724628623
absorption of pteroylglutamate and dietary folates in man. 19724628665
enumeration of certain lactic acid bacteria from wiltshire bacon-curing brines. 19724555288
malt extract as protective medium for lactic acid bacteria in cryopreservation. 19724563585
regulation of c metabolism by l-methionine in saccharomyces cerevisiae.1. the concentrations of folate derivatives in aerobic cultures of saccharomyces cerevisiae (a.t.c.c. 9763) were determined by microbiological assay employing lactobacillus casei (a.t.c.c. 7469) and pediococcus cerevisiae (a.t.c.c. 8081). cells cultured in media lacking l-methionine contained higher concentrations of folate derivatives than cells grown in the same media supplemented with 2.5mumol of l-methionine/ml. the concentrations of highly conjugated derivatives were also decreased by suppl ...19724198357
[use of the fluorescent antibody method for microbiological control in the food-processing industry (review)]. 19724207751
cross-resistance and collateral susceptibility to antifolic antimalarial compounds.series of strains of streptococcus faecium atcc 8043, lactobacillus casei atcc 7469, and pediococcus cerevisiae atcc 8081 with increasing resistance to the active antifolate antimalarial drugs chlorguanide triazine (cgt), pyrimethamine (pm), and trimethoprim (tmp) were isolated. these mutant strains, stable for at least 3 to 5 years, were examined for cross-resistance and collateral susceptibility to the above compounds and to methotrexate (mtx). generally, they exhibited cross-resistance to all ...19724208273
trimethoprim-sulphamethoxazole effect on folate metabolism. 19734199529
aldolase of lactic acid bacteria: a case history in the use of an enzyme as an evolutionary marker. 19734203394
[the process of production of various aromatic substances by the lactic acid microbes ped. cerevisiae and lact. plantarum when cultured together for the purpose of using them in production of boiled sausage]. 19734203934
identification and measurement of the folates in sheep liver.1. methods are described for the extraction, separation by ion-exchange chromatography and estimation by microbiological assay of the folates in sheep liver. 2. injection of [2-(14)c]-pteroylglutamate into a sheep fed on a stock diet led to extensive labelling of chromatographically separable liver folates. about 12% of the label in the liver could not be extracted by the method used. 3. liver folates were examined in five ewes fed on restricted amounts of a diet of wheaten hay-chaff and gluten ...19734204321
folic acid metabolism in vitamin b12-deficient sheep. depletion of liver folates.1. metabolism of folate was studied in six ewes in an advanced state of vitamin b(12) deficiency as judged by voluntary food intake and in their pair-fed controls receiving vitamin b(12). a group of four animals that were maintained throughout the experiment at pasture was also studied. 2. after 34-40 weeks on the cobalt-deficient diet urinary excretion of formiminoglutamate by four deficient animals was about 3.2mmol/day and this was not significantly decreased by injection of three of them wit ...19734204322
studies of folic acid compounds in nature. 3. folic acid compounds in cabbage. 19734204493
plasmodium berghei: folic acid levels in mouse erythrocytes. 19734197972
glycerol teichoic acid as a common antigenic factor in lactobacilli and some other gram-positive organisms. 19734121097
folate distribution in fruit juices. 19734631389
trimethoprim-sulphamethoxazole and folate metabolism. 19734632643
effect of selected lactic acid bacteria on growth of staphylococcus aureus and production of enterotoxin.representative strains of 15 species of lactic acid bacteria were examined for their ability to influence growth of staphylococcus aureus and production of enterotoxin in associative culture. among the organisms used as effectors the streptococci were most inhibitory, followed by pediococcus cerevisiae. the lactobacilli and leuconostoc citrovorum were not inhibitory to growth and only slightly inhibitory to production of enterotoxin. enterotoxin was detected in all cultures in which the populati ...19734633430
the effect of antibiotics on synthesis of mucopeptide and teichoic acid by pediococcus cerevisiae and by a substrain that requires methicillin for growth. 19734772092
uptake and metabolism of folate by a folate-utilizing mutant of pediococcus cerevisiae (8081-s) and the parent strain (8081). 19734540523
carrier-mediated transport of folate in a mutant of pediococcus cerevisiae.a mutant strain of pediococcus cerevisiae (p. cerevisiae/pteglu) was isolated which grows on low-folate (pteglu) concentrations (200 pg/ml). the growth response of the parent and mutant strains to folinate (5-cho-h(4)pteglu) was the same. the transport of (14)c-pteglu by p. cerevisiae/pteglu was temperature-dependent (q(10) between 27 c and 37 c was about 2), energy-dependent, and ph-dependent and was inhibited by iodoacetate, 2,4-dinitrophenol, potassium fluoride, and sodium azide. the uptake o ...19734540970
medium for the selective enumeration of lactic acid bacteria from foods.a nitrite actidione polymyxin agar was developed for the enumeration of lactic acid bacteria. it was effective in recovering organisms from pure cultures and from foods.19734356465
effect of variations in conditions of incubation upon inhibition of staphylococcus aureus by pediococcus cerevisiae and streptococcus lactis.the effects of ph, temperature, proportion of staphylococcus aureus in the inoculum, various strains of effector organism, and various strains of s. aureus were examined for their influence on interactions between staphylococci and effector organisms in associative culture. in general, small changes in ph had little effect upon either growth of s. aureus or production of enterotoxin in associative culture. inhibition of growth of s. aureus caused by effector organisms was much greater at 25 than ...19734632847
wall synthesis by pediococcus cerevisiae and by a substrain that requires methicillin for growth.pediococcus cerevisiae and p. cerevisiae crd synthesized peptidoglycan when incubated in a wall synthesis solution inadequate to allow growth.19734745426
studies on the metabolic interrelationship between biotin and folic acid in rats. 19744215873
lipids of pediococcus cerevisiae and some methicillin-resistant substrains. 19744545199
inhibition of growth of pediococcus cerevisiae by polycyclic hydrocarbon-cysteine conjugates. 19744814142
quinazolines. 12. 1,3-diaminobenzo(f)quinazolines containing long-chain alkyl or chloro substituents on the central ring. synthesis and biological evaluation as candidate antifolate and antimalarial agents. 19744606974
folic acid metabolism in vitamin b12-deficient sheep. effects of injected methionine on liver constituents associated with folate metabolism.1. the effects of injected l-methionine (2g every second day for 28 days) on liver folates and other constituents of liver associated with folate metabolism were studied in vitamin b(12)-deficient ewes and their pair-fed controls receiving vitamin b(12). the dose rate of methionine used was sufficient to restore almost to normal the elevated excretion in the urine of formiminoglutamate in the deficient animals. 2. liver folates active for lactobacillus casei, streptococcus faecalis r and pedioco ...19744155289
synthesis and antifolate activity of 10-deazaminopterin. 19744208480
[the presence of malic enzyme and malo-lactic enzyme in various lactic acid bacteria (author's transl)]. 19744209828
rapid transfer of folic acid from blood to bile in man, and its conversion into folate coenzymes and into a pteroylglutamate with little biological activity. 19744212300
studies of folate compounds in nature. folate compounds in rat kidney and red blood cells. 19744212359
development of a selective enterococcus medium based on manganese ion deficiency, sodium azide, and alkaline ph.rogosa broth, without its salt supplement and dissolved in deionized water, was adapted for the selective isolation and enumeration of enterococci. this medium supported good growth of enterococci, but it suppressed growth of other lactic acid bacteria. the sensitivity and specificity of the medium were tested after addition of various increasing concentrations of nan(3) against known strains of enterococci and other bacteria. many strains of streptococcus faecium showed low azide tolerance; opt ...19744214072
variations in the quaternary structure of three lactic acid bacteria aldolases. evidence for the existence of a class i and class ii aldolase in lactobacillus casei. 19744214814
biological ensiling of sugarcane tops. vii. isolation and characteristics of lactic acid bacteria. 19744215715
dihydrofolate reductase in pediococcus cerevisiae strains susceptible and resistant to amethopterin.a mutant strain of pediococcus cerevisiae was obtained which had 1,000-fold greater resistance to amethopterin than the p. cerevisiae/pteglu strain. the dihydrofolate reductase level of the mutant was about 60-fold higher than that of the parent. the mutant accumulated folate, though less efficiently than its parent.197415830489
microbial inhibition by an isolate of pediococcus from cucumber brines.we reported earlier that pediococcus cerevisiae fbb-61 inhibited lactobacillus plantarum fbb-67 in mixed species inoculation used for the fermentation of brined cucumbers. herein, 16 isolates of the pediococcus genus from various sources were tested for inhibitory activity against l. plantarum and other microorganisms by a seeded-agar screening technique. only two of the 16 isolates gave consistent and distinctive zones of inhibition, and both were isolated from fermenting cucumber brines on sep ...197516350041
lipoic acid content of escherichia coli and other microorganisms.a mutant strain of escherichia coli k-12 requiring lipoic acid. w1485lip 2 (atcc 25645), was used to develop a turbidimetric assay for lipoic acid and a polarographic assay based on the oxidation of pyruvate by suspensions of lipoic acid-deficient organisms. the turbidmetric assay was more sensitive with a working range equivalent to 0.2-2.0 ng of dl-alpha-lipoic acid compared with 5-50 ng for the polarographic method. the mutant responded equally to racemic mixtures of alpha-lipoic acid, beta-l ...1975814874
assay for citrovorum factor (nsc-3590) in the presence of methotrexate (nsc-740).treatment of certain malignancies with high-dose methotrexate/citrovorum factor rescue has recently been adopted as an effective regimen. a microbiologic assay capable of detecting citrovorum factor in the presence of massive amounts of methotrexate has been developed using a strain of pediococcus cerevisiae resistant to methotrexate. the assay described in this paper is an inexpensive and rapid method of studying the distribution kinetics of citrovorum factor.19751081908
deterioration of high-moisture corn.two small, leaky silos were filled with normal high-moisture corn (hmc), and two with hmc severely infested by helminthosporium maydis. counts of mesophilic bacteria, lactobacilli, coliforms, yeasts, and molds were made on corn samples as received and periodically thereafter during 220 days of storage. temperature and gas levels also were monitored. sequential changes in the populations of lactobacilli, yeasts, and molds were determined during spoilage of hmc. these population changes were compa ...19751147609
pediococcus acidilactici ncib 6990, a new test organism for microbiological assaying of pantothenic acid. 19751194131
production of racemic lactic acid in pediococcus cerevisiae cultures by two lactate dehydrogenases.nicotinamide adenine dinucleotide (nad)-dependent d(minus)-and l(plus)-lactate dehydrogenases have been partially purified 89- and 70-fold simultaneously from cell-free extracts of pediococcus cerevisiae. native molecular weights, as estimated from molecular sieve chromatography and electrophoresis in nondenaturing polyacrylamide gels, are 71,000 to 73,000 for d(minus)-lactate dehydrogenase and 136,000 to 139,000 for l(plus)-lactate dehydrogenase. electrophoresis in sodium dodecyl sulfate-contai ...1975234418
pediococcus cerevisiae mutant with altered transport of folates.a pediococcus cerevisiae mutant that actively accumulated folate (pteglu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-ch3-h4pteglu) and amethopterin. most of the 5-ch3-h4pteglue accumulated through a glucose- and temperature-dependent process, and a concentrative uptake was also found in gluocse-starved cells and in cells incubated at oc. about 75% of the accumulated 5-ch3-h4pteglu exchanged with amethopterin. in contr ...19751080150
[controlled fermentation of pickles using pure bacterial strains]. 19751056609
folate concentration and pattern in bovine milk. 1975807607
experimental production of elevated serum folate in dogs with intestinal blind loops. ii. nature of bacterially produced folate coenzymes in blind loop fluid.jejunal and ileal blind loops were constructed in separate groups of dogs and the folate coenzymes present in these loops were investigated. although only those dogs with high blind loops had appreciably elevated folate levels active for lactobacillus casei but not streptococcus faecalis, every blind loop appeared to contain four folate compounds--5-formyltetrahydrofolic acid, 5-methyltetrahydrofolic acid, tetrahydrofolic acid and a compound tentatively identified as 5-formyltetrahydrofolic trig ...1975807096
studies of folic acid compounds in nature. iv. folic acid compounds in soybeans and cow milk.folate compounds in soybean and cow's mild were identified by sephadex chromatography and differential microbiological assaymsoybean contained mainly monoglutamates (ca. 52%), some diglutamates (ca. 16%), and polyglutamates (pentaglutamates representing the major portion). 5-cho-h4pteglun(1-6) constituted 65-70% of total folate activity; cow's milk contained monoglutamates (60%) and polyglutamates (ranging from di- to hepta-conjugates). in contrast to soybean, 90-95% of milk folate was in the 5- ...1975804977
transport and metabolism of folates by bacteria.transport of labeled folic acid (pteglu), pteroylpolyglutamates (pteglu3-5), 5-methyl-tetrahydrofolate (5-methyl-h4pteglu), and methotrexate in late-log phase cells of lactobacillus casei was active, and subject to inhibition by unlabeled pteroylmonoglutamates, pteroylpolyglutamates, and iodoacetate, but not glutamate or glutamate dipeptides. pteroylpolyglutamates were transported without prior hydrolysis and shared a common uptake system with pteroylmonoglutamates. the affinity and maximum velo ...1975234963
antimetabolites of pantothenic acid, ureido- and carbamoyl-derivatives.pantothenic acid analogs have been synthesized that contain alkyl and/or arylureido and carbamate functions in the beta-alanyl portion of the amide moiety of the vitamin. the analogs are inhibitory to growth of lactic acid bacteria at concentrations as low as 0.6 mug/ml, and the inhibitions are competitively reversed by supplements of pantothenic acid. the carbamate derivatives are more toxic to growth of lactobacillus plantarum 8014 and pediococcus cerevisiae 8042 than the ureido analogs, and c ...19751228973
formation of acetaldehyde from threonine by lactic acid bacteria.group n streptococci were found to cleave threonine to form acetaldehyde and glycine. threonine aldolase, the enzyme catalysing this reaction, was found in all strains except streptococcus cremoris z8, an organism which had been shown previously to have a nutritional requirement for glycine. the enzyme was strongly inhibited by glycine and cysteine. the inhibition showed characteristics of allosteric inhibition and was ph-dependent. inhibition by glycine, but not by cysteine, was highly specific ...19761262564
metabolism of dihydroaminopterin and the influence of aminopterin and related derivatives on the metabolism of (-)-5-formyl-tetrahydrofolic acid in pediococcus cerevisiae.[3h]dihydroaminopterin, but not [3h]methotrexate or [3h]aminopterin, was rapidly taken up by resting cell of pediococcus cerevisiae and inhibited the uptake of (-)-5-formyl-tetrahydrofolic acid to more than 90% at a concentration of 25 mum. on the other hand 5-formyl-tetrahydrofolic acid inhibited the uptake of dihydroaminopterin to a similar extent. dihydroaminopterin was metabolized by pediococcus cerevisiae, but not by lactobacillus casei, to a compound tentatively identified as dihydroaminop ...19761247588
acetaldehyde: an intermediate in the formation of ethanol from glucose by lactic acid bacteria.group n streptococci formed acetaldehyde and ethanol from glucose. as the enzymes aldehyde dehydrogenase, phosphotransacetylase and acetate kinase were present this would enable these organisms to reduce acetyl-coa to acetaldehyde and convert acetyl-coa to acetyl phosphate and acetate. a pentose phosphate pathway which converted ribose-5-phosphate to glyceraldehyde-3-phosphate was also present. acetaldehyde could not be formed via the hexose monophosphate shunt or by direct decarboxylation of py ...1976177470
comparison of chemical and microbiological methods in the estimation of methionine in cowpea (vigna unguiculata) seeds.1. meals were prepared from the seeds of fifteen varieties of cowpea (vigna unguiculata), one of lima bean (phaseolus lunatus) and one of yam bean (sphenostylis stenocarpa), and their methionine content was determined by six different methods. 2. total methionine content was determined by two chemical methods (ion-exchange chromatography and a colorimetric procedure) and by two microbiological methods. the 'available' methionine content was determined by microbiological assay with streptococcus ...1976821514
endocarditis and osteomyelitis caused by aerococcus viridans. 19761084468
the nutritional requirements of methicillin-dependent and -resistant strains of pediococcus cerevisiae.a new methicillin-dependent and -resistant substrain (called mrd) of pediococcus cerevisiae was developed by serial passages followed by replica-plating. other methicillin-resistant, but not -dependent, substrains were isolated after treatment of the same parent strain with a mutagen. a methicillin-independent partial revertant, still resistant to the drug, was isolated from the original methicillin-dependent and -resistant substrain (crd) developed several years ago. the requirements of some of ...19761082009
resistance to pediococcus cerevisiae to amethopterin as a consequence of changes in enzymatic activity and cell permeability. ii. permeability changes to amethopterin and other folates in the drug-resistant mutant.the accumulation of amethopterin in a pediococcus cerevisiae strain resistant to this analogue was about 30% of that in p. cerevisiae/pteglu, the sensitive parent. the uptake in the resistant strain was strictly glucose dependent, whereas in the sensitive parent about 16% accumulation occurred in absence of glucose. the transport in both strains was inhibited by iodoacetate and kf. amethopterin uptake exhibited saturation kinetics with an apparent km of 5 mum in p. cerevisiae/amr and 0.5 mum in ...19766055
resistance of pediococcus cerevisiae to amethopterin as a consequence of changes in enzymatic activity and cell permeability. i. dihydrofolate reductase, thymidylate synthetase and formyltetrahydrofolate synthetase in amethopterin-resistant and -sensitive strains of pediococcus cerevisiae.pediococcus cerevisiae/amr, resistant to amethopterin, possesses a higher dihydrofolate reductase (5, 6, 7, 8-tetrahydrofolate: nadp+ oxidoreductase, ec 1.5.1.3) activity than the parent, a folate-permeable and thus amethopterin-susceptible strain and than the wild-type. the properties of dihydrofolate reductase from the three strains have been compared. temperature, ph optima, heat stability, as well amethopterin binding did not reveal significant differences between the enzymes from the suscep ...19766054
effects of volatile bacterial metabolites on the growth, sporulation and mycotoxin production of fungi. 1976817083
an improved procedure for the assay of available lysine and methionine in feedstuffs using tetrahymena pyriformis w.1. a study was made of the assay of available lysine and available methionine using tetrahymena pyriformis w, as applied to a variety of protein-rich feedstuffs. 2. results were strongly influenced by the conditions under which the analyses were done. with most of the test samples predigestion with papain caused a large increase in the values obtained. 3. an improved assay procedure was developed which included enzymic predigestion of the test samples, "all-in" sterilization of the medium consti ...1976817733
delay in metabolism of parenteral folinic acid to 5-methyltetrahydrofolate in man. 1976826436
folate coenzyme forms in fibroblasts from patients deficient in 5,10-methylenetetrahydrofolate reductase. 1976826437
[on the metabolism of amino acids by lactic acid bacteria isolated from wine (author's transl)].the changes in the concentrations of amino acids in the culture medium of lactic acid bacteria were determined by ion exchange chromatography after growth of 105 strains that were mainly isolated from wine. after growth of lactic acid bacteria a small but not significant decrease in the concentration of most amino acids is observed. there is apparently no difference between essential or not essential amino acids. certain bacterial strains decompose the amino acids arginine, glutamic acid, histid ...1976973463
significance of starter cultures for raw sausage aging in view of food and nutrition hygiene.the authors studied the effects of liquid starter cultures on the survival of pathogenic germs. it was found that the foreign bacteria tested differed in growth limitation which is obviously dependent on acidity and the amount of lactic acid produced by fermentation. since the pathogenic bacteria differ in the ability to survive, it is imperative to observe strict hygienic measures in preparing starter cultures and to use absolutely sterile monocultures for raw sausage ageing.19768718
aldolases of the lactic acid bacteria. demonstration of immunological relationships among eight genera of gram positive bacteria using an anti-pediococcal aldolase serum.reciprocal qualitative and quantitative immunological experiments employing an anti-pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the streptococcus faecalis fructose diphosphate aldolase. the extent of immunological relatedness observed between the lactobacillus and pediococcus aldolases was markedly gses indicating that the pediococci share closer phylogenetic ties with th ...197665160
aspartate aminotransferase of pediococcus cerevisiae.a five-step procedure is described for preparing highly purified aspartate aminotransferase (l-aspartate: 2-oxoglutarate aminotransferase, ec.2.6.1.1) from cell-freee enzyme extracts of pediococcus cerevisiae. an overall purification of 130-fold was achieved. some of p. cerevisiae aspartate aminotransferase properties were studied, i.s. ph optimum (7.8--8.0), optimum of temperature (37 degrees), michaelis constans for 4 enzyme substrates and substrate specificity of enzyme. the enzyme is very th ...197767756
microbiologic assays of cancer chemotherapeutic agents.among various assay methods available for the study of drug distribution kinetics in cancer chemotherapy, a method that determines the drug in its active form is the most desirable. the microbiologic assay method, with its rapid, simple, and inexpensive procedures, fulfills such a requirement. besides the two commonly used chemotherapeutic agents, methotrexate and cytosine arabinoside, microbiologic assays are available for other chemotherapeutic agents, such as 6-mercaptopurine, 5-fluorouracil, ...1977406993
Displaying items 101 - 200 of 1105