| [an epidemic of coxsackie b5 meningitis in a newborn nursery]. | | 2016 | 5050641 |
| [instrumentation physics in medicine: capacitance and dielectric constants]. | | 2013 | 3820784 |
| colotomy with minimum resection for advanced irreducible intussusception. | | 2006 | 1501020 |
| dynamic computed tomography as an ancillary screening test for traumatic thoracic aortic injuries: a critical review. | while the divergence of opinion on the matter of diagnostic modalities will probably continue, dynamic computed tomography scans should be considered as a necessary study in assessing a possible traumatic thoracic aortic injury. the use of this modality will decrease the need for thoracic angiograms and invariably increase the yield. | 2007 | 1920521 |
| alzheimer's senile dementia: a radical case of cephaloconiosis. | | 2012 | 3374740 |
| [the conjunctiva]. | | 2014 | 4227861 |
| identification of skull from its suture pattern. | the examination of 320 skulls collected at random revealed that the ectocranial suture patterns in them are highly individualistic and that no two skulls can ever have an identical pattern. the possibility of these suture patterns being recorded incidentally in routine diagnostic skull radiographs was verified by examining the skull skiagrams preserved in radiology departments. a positive method of identification of the skull is suggested by the comparison of radiographic with visible skull sutu ... | 2014 | 3988197 |
| the solace of a dream. | | 2013 | 3848472 |
| [the treatment of scleroderma in children at the sochi-matseta health resort]. | | 2014 | 4240841 |
| closed injuries of the ankle. | | 2014 | 4200146 |
| cardiac amyloidosis: combined use of two-dimensional echocardiography and electrocardiography in noninvasive screening before biopsy. | cardiac amyloidosis (ca) presenting with intractable congestive heart failure, electrocardiographic (ecg) normal or low voltage, and conduction or rhythm disturbances, is rapidly fatal. during life, ca often mimics other cardiomyopathies so that definitive diagnosis depends on demonstration of amyloid on myocardial biopsy. on two-dimensional echocardiography (2-d echo), nonspecific features, such as increased ventricular wall thicknesses, predominant diastolic dysfunction, and diffuse myocardial ... | 2014 | 4053429 |
| [comparative cutaneous measurement of oxygen pressure (pcuo2) in healthy individuals and in patients with progressive scleroderma]. | a modified method combining the two methods of measuring either tissue oxygen tension (po2) or transcutaneous po2, which have already been proven in other clinical fields, is presented for application in particular skin diseases. this so-called "cutaneous po2 measurement" (pcuo2) is based on measurements from commercially available transcutaneous po2 devices, but produces moderate hyperemia through the lower electrode temperature (e.g., 40.5 degrees c) instead of maximal hyperemia (by 44 degrees ... | 2014 | 3957666 |
| treatment of cancer of maxilla. | | 2015 | 4661974 |
| [remembering prof. bernardo lópez martínez]. | | 2015 | 4619534 |
| [chemical and pharmacological research on derivatives of 1h-naphtho(2,1-b)pyran. 3. synthesis of 2,2'-methylenbis-(1-oxo-3-dialkylamino-1h-naphthol(2,1-b)pyran)]. | | 2016 | 4827185 |
| transcriptome analysis of the intracellular facultative pathogen piscirickettsia salmonis: expression of putative groups of genes associated with virulence and iron metabolism. | the intracellular facultative bacteria piscirickettsia salmonis is one of the most important pathogens of the chilean aquaculture. however, there is a lack of information regarding the whole genomic transcriptional response according to different extracellular environments. we used next generation sequencing (ngs) of rna (rna-seq) to study the whole transcriptome of an isolate of p. salmonis (favet-inbiogen) using a cell line culture and a modified cell-free liquid medium, with or without iron s ... | 2016 | 28033422 |
| evidence of the presence of a functional dot/icm type iv-b secretion system in the fish bacterial pathogen piscirickettsia salmonis. | piscirickettsia salmonis is a fish bacterial pathogen that has severely challenged the sustainability of the chilean salmon industry since its appearance in 1989. as this gram-negative bacterium has been poorly characterized, relevant aspects of its life cycle, virulence and pathogenesis must be identified in order to properly design prophylactic procedures. this report provides evidence of the functional presence in p. salmonis of four genes homologous to those described for dot/icm type iv sec ... | 2013 | 23383004 |
| impairment of doctors: are beginning medical students psychologically vulnerable? | the hypothesis that the stressful nature of medical training is a major cause of impairment of doctors is explored in this study by examining whether changes in psychological adjustment occur during the first year, the first year is stressful, and sex differences in psychological adjustment or stress occur. all first-year medical students at an australian university completed self-rating measures of psychiatric symptomatology, ego-resilience, depressive mood and role conflict, support and confid ... | 2014 | 4069020 |
| piscirickettsia salmonis imbalances the innate immune response to succeed in a productive infection in a salmonid cell line model. | piscirickettsia salmonis is a facultative intracellular bacterium that causes the disease called "salmon rickettsial syndrome". attempts to control this disease have been unsuccessful, because existing vaccines have not achieved the expected effectiveness and the antibiotics used fail to completely eradicate the pathogen. this is in part the product of lack of scientific information that still lacks on the mechanisms used by this bacterium to overcome infected-cell responses and survive to induc ... | 2016 | 27723816 |
| [determination of volatile water-soluble organic compounds in hermetically sealed objects]. | | 2015 | 4652090 |
| a novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions. | piscirickettsia salmonis is the bacterium that causes piscirickettsiosis, a systemic disease of salmonid fish responsible for significant economic losses within the aquaculture industry worldwide. the growth of the bacterium for vaccine formulation has been traditionally accomplished by infecting eukaryotic cell lines, a process that involves high production costs and is time-consuming. recent research has demonstrated that it is possible to culture pure p. salmonis in a blood containing (cell-f ... | 2013 | 24039723 |
| genomic-based restriction enzyme selection for specific detection of piscirickettsia salmonis by 16s rdna pcr-rflp. | the gram negative facultative bacterium p. salmonis is the etiological agent of salmonid rickettsial septicaemia (srs), a severe disease that causes important economic losses in the global salmon farmer industry. despite efforts to control this disease, the high frequency of new epizootic events indicate that the vaccine and antibiotics treatments have limited effectiveness, therefore the preventive and diagnostic approaches must be improved. a comparison of several methodologies for srs diagnos ... | 2016 | 27242682 |
| bicaudate index in computerized tomography of huntington disease and cerebral atrophy. | ventricular sizes on computerized tomographic (ct) scans were compared in seven patients with huntington disease, 20 patients with cerebral atrophy, and 20 normal controls. the bicaudate index--the ratio of the width of both lateral ventricles at the level of the heads of the caudate nuclei to the distance between the outer tables of the skull at the same level--significantly discriminated among the three groups. the bicaudate indices were: huntington disease 0.209 +/- 0.007, cerebral atrophy 0. ... | 2003 | 152416 |
| transcriptional response of atlantic salmon families to piscirickettsia salmonis infection highlights the relevance of the iron-deprivation defence system. | piscirickettsiosis or salmonid rickettsial septicaemia (srs) is a bacterial disease that has a major economic impact on the chilean salmon farming industry. despite the fact that piscirickettsia salmonis has been recognized as a major fish pathogen for over 20 years, the molecular strategies underlying the fish response to infection and the bacterial mechanisms of pathogenesis are poorly understood. we analysed and compared the head kidney transcriptional response of atlantic salmon (salmo salar ... | 2015 | 26141111 |
| comparative analysis of membrane vesicles from three piscirickettsia salmonis isolates reveals differences in vesicle characteristics. | membrane vesicles (mvs) are spherical particles naturally released from the membrane of gram-negative bacteria. bacterial mv production is associated with a range of phenotypes including biofilm formation, horizontal gene transfer, toxin delivery, modulation of host immune responses and virulence. this study reports comparative profiling of mvs from bacterial strains isolated from three widely disperse geographical areas. mass spectrometry identified 119, 159 and 142 proteins in mvs from three d ... | 2016 | 27764198 |
| antimicrobial activity of trout hepcidin. | hepcidin is an antimicrobial peptide and a hormone produced mostly the liver. it is a cysteine-rich peptide with a highly conserved β-sheet structure. recently, we described the hepcidin expression in liver of rainbow trout and its inducibility by iron overloading and lipopolysaccharide (lps). thus, in this work, we focused in analyzing the importance of the peptide conformation associated to its oxidative state in the antimicrobial activity. this peptide showed a α-helix conformation in reduced ... | 2014 | 24794583 |
| in sílico identification and characterization of putative dot/icm secreted virulence effectors in the fish pathogen piscirickettsia salmonis. | piscirickettsia salmonis seriously affects the chilean salmon industry. the bacterium is phylogenetically related to legionella pneumophila and coxiella burnetii, sharing a dot/icm secretion system with them. although it is well documented that l. pneumophila and c. burnetii secrete different virulence effectors via this dot/icm system in order to attenuate host cell responses, to date there have been no reported virulence effectors secreted by the dot/icm system of p. salmonis. using several an ... | 2016 | 26706346 |
| the link between nutrition and family planning. | | 2004 | 415715 |
| mr imaging of epidermoid cysts. | nine patients with epidermoid cysts, five of them pathologically proved, were evaluated with mr imaging. six patients also had ct. the cases were reviewed to evaluate the mr appearance of epidermoid cysts and to compare the mr findings with those of ct. the epidermoid cysts demonstrated low-signal intensity on t1-weighted mr images and hyperintensity on t2-weighted images. in five cases the cysts appeared heterogeneously iso- to hyperintense on the intermediate echo, and were surrounded by a thi ... | 2006 | 2494855 |
| ribavirin enters cerebrospinal fluid. | | 2010 | 2873344 |
| in the shadow: the emerging role of long non-coding rnas in the immune response of atlantic salmon. | the genomic era has increased the research effort to uncover how the genome of an organism, and specifically the transcriptome, is modulated after interplaying with pathogenic microorganisms and ectoparasites. however, the ever-increasing accessibility of sequencing technology has also evidenced regulatory roles of long non-coding rnas (lncrnas) related to several biological processes including immune response. this study reports a high-confidence annotation and a comparative transcriptome analy ... | 2017 | 28373064 |
| varicella pneumonia. treatment with tracheostomy and mechanical endotracheal positive-pressure ventilation. | | 2016 | 4860512 |
| [research--the source of medical progress]. | | 2013 | 3842698 |
| information processing and automatic diagnosis of circulatory system--introductory remarks from engineer's point of view. | | 2015 | 4610203 |
| identification and genetic characterization of piscirickettsia salmonis in native fish from southern chile. | piscirickettsia salmonis is the etiological agent of piscirickettsiosis, a severe disease causing high mortalities in salmonids. this bacterium has been previously identified and isolated in all cultivated salmonids in chile and worldwide, including salmo salar, oncorhynchus kisutch, and o. mykiss, in addition to being found in non-salmonid species such as dicentrarchus labrax and atractoscion nobilis. in this study, the 16s rrna gene and intergenic spacer its-1 of p. salmonis were amplified by ... | 2015 | 26290508 |
| identification, characterization and modulation of ferritin-h in the sub-antarctic notothenioid eleginops maclovinus challenged with piscirickettsia salmonis. | ferritin is a major iron storage protein essential not only in the infectious process, but also in any circumstance generating oxidative stress. in this study, the cdna coding sequence of ferritin-h was obtained from the sub-antarctic notothenioid fish eleginops maclovinus through transcriptomic analysis of the head kidney. this sequence contained a 534 bp open reading frame that coded for a 177 amino acid protein with a molecular weight of 20,786.2 da and a theoretical pi of 5.56. the protein d ... | 2017 | 28336188 |
| the genome sequence of an oxytetracycline-resistant isolate of the fish pathogen piscirickettsia salmonis harbors a multidrug resistance plasmid. | the amount of antibiotics needed to counteract frequent piscirickettsiosis outbreaks is a major concern for the chilean salmon industry. resistance to antibiotics may contribute to this issue. to understand the genetics underlying piscirickettsia salmonis-resistant phenotypes, the genome of ay3800b, an oxytetracycline-resistant isolate bearing a multidrug resistance plasmid, is presented here. | 2017 | 28153906 |
| molecular cloning of salmo salar toll-like receptors (tlr1, tlr22, tlr5m and tlr5s) and expression analysis in shk-1 cells during piscirickettsia salmonis infection. | in fish, the innate immune system is the primary response against infection. toll-like receptors (tlrs) recognize pathogens through pathogen-associated molecular patterns (pamps), and some target molecules of tlrs are homologous between fish and mammals. piscirickettsia salmonis is one of the main pathogens affecting the salmon industry in chile. better knowledge of mechanisms underlying its invasive capacity and recognition of target cells is crucial for vaccine development. therefore, salmo sa ... | 2016 | 25903926 |
| identification of genomic islands in chilean piscirickettsia salmonis strains and analysis of gene expression involved in virulence. | piscirickettsia salmonis, an agent of piscirickettsiosis, is the cause of major losses in the chilean salmon industry. we identified, characterized and bioinformatically analysed genomic islands in field strains of p. salmonis, using the bioinformatic software pips, that uses the characteristics of the islands of pathogenicity to identify them. we analysed nine partially sequenced genomes in different new field strains, and compared them with the lf-89 (type strain) genome, selecting a genomic i ... | 2017 | 28150307 |
| genomic predictions can accelerate selection for resistance against piscirickettsia salmonis in atlantic salmon (salmo salar). | salmon rickettsial syndrome (srs) caused by piscirickettsia salmonis is a major disease affecting the chilean salmon industry. genomic selection (gs) is a method wherein genome-wide markers and phenotype information of full-sibs are used to predict genomic ebv (gebv) of selection candidates and is expected to have increased accuracy and response to selection over traditional pedigree based best linear unbiased prediction (pblup). widely used gs methods such as genomic blup (gblup), snpblup, baye ... | 2017 | 28143402 |
| comparative genome analysis of two isolates of the fish pathogen piscirickettsia salmonis from different hosts reveals major differences in virulence-associated secretion systems. | outbreaks caused by piscirickettsia salmonis are one of the major threats to the sustainability of the chilean salmon industry. we report here the annotated draft genomes of two p. salmonis isolates recovered from different salmonid species. a comparative analysis showed that the number of virulence-associated secretion systems constitutes a main genomic difference. | 2014 | 25523762 |
| prevalence, geographic distribution and phenotypic differences of piscirickettsia salmonis em-90-like isolates. | early reports accounted for two main genotypes of piscirickettsia salmonis, a fish pathogen and causative agent of piscirickettsiosis, placing the single isolate em-90 apart from the prototypic lf-89 and related isolates. in this study, we provide evidence that, contrary to what has been supposed, the em-90-like isolates are highly prevalent and disseminated across chilean marine farms. molecular analysis of 507 p. salmonis field isolates derived from main rearing areas, diverse hosts and collec ... | 2017 | 28075013 |
| growth characteristics of the intracellular pathogen, piscirickettsia salmonis, in tissue culture and cell-free media. | piscirickettsia salmonis is an intracellular bacterium that was first isolated and identified in fish cells. several types of cell lines have been explored for their ability to provide the bacterium with a host cell to replicate in. tissue culture has been used for growth and cultivation for nearly two decades, until the facultative nature of p. salmonis was confirmed upon the development of blood- and cysteine-based agar. since then, research has continued to drive the creation of novel agar an ... | 2016 | 28026007 |
| search and analysis of genes involved in antibiotic resistance in chilean strains of piscirickettsia salmonis. | piscirickettsia salmonis is the pathogen causing piscirickettsiosis. for treatment, the industry mainly uses oxytetracycline and florfenicol, so it is essential to understand the degree of susceptibility of this pathogen to these drugs. but this is still unknown for a large number of p. salmonis strains, as are the molecular mechanisms responsible for greater or lesser susceptibility. however, genes that confer resistance to these antimicrobials have been reported and characterized for this and ... | 2016 | 27982445 |
| stocking density and piscirickettsia salmonis infection effect on patagonian blennie (eleginops maclovinus, cuvier 1830) skeletal muscle intermediate metabolism. | the need to expand aquaculture production has led to other fish to be considered as potential species for culture, such as the sub-antarctic notothenioid eleginops maclovinus (valenciennes, 1830). the aim of this study was to determine the cumulative effect of density and pathogen infection by protein extract of piscirickettsia salmonis on skeletal muscle metabolism. in a first experiment, specimens were submitted to three different stocking densities: (1) 3.1 kg m(-3), (2) 15 kg m(-3) and (3) 6 ... | 2014 | 25034336 |
| whole-genome comparative analysis of the pathogen piscirickettsia salmonis. | the intracellular pathogen piscirickettsia salmonis is the etiological agent of piscirickettsiosis, the most important bacterial disease that affects the chilean salmon industry. despite its importance, little is known regarding the biology of the pathogen. in this study, recently published sequencing data was used in order to characterize the genome of p. salmonis, defining groups of genes associated with bacterial processes such as, invasion and intracellular survival. moreover, one chilean p. ... | 2016 | 27939153 |
| immunization strategies against piscirickettsia salmonis infections: review of vaccination approaches and modalities and their associated immune response profiles. | salmonid rickettsial septicemia (srs) is a serious, infectious disease in chilean salmon farming caused by piscirickettsia salmonis, causing heavy losses to the salmonid industry. p. salmonis belongs to the gammaproteobacteria, order thiotrichales. srs was first described in chile in 1989, and infection with p. salmonis has since been described from a high number of fish species and in several geographic regions globally. p. salmonis infection of salmonids causes multifocal, necrotic areas of in ... | 2016 | 27917172 |
| novel insights into the response of atlantic salmon (salmo salar) to piscirickettsia salmonis: interplay of coding genes and lncrnas during bacterial infection. | despite the high prevalence and impact to chilean salmon aquaculture of the intracellular bacterium piscirickettsia salmonis, the molecular underpinnings of host-pathogen interactions remain unclear. herein, the interplay of coding and non-coding transcripts has been proposed as a key mechanism involved in immune response. therefore, the aim of this study was to evidence how coding and non-coding transcripts are modulated during the infection process of atlantic salmon with p. salmonis. for this ... | 2016 | 27818337 |
| genome-scale metabolic reconstruction for the insidious bacterium in aquaculture piscirickettsia salmonis. | piscirickettsia salmonis is a fish bacterium that causes the disease piscirickettsiosis in salmonids. this pathology is partially controlled by vaccines. the lack of knowledge has hindered its culture on laboratory and industrial scale. the study describes the metabolic phenotype of p. salmonis in culture. this study presents the first genome-scale model (ipf215) of the lf-89 strain of p. salmonis, describing the central metabolic pathway, biosynthesis and molecule degradation and transport mech ... | 2017 | 27788423 |
| piscirickettsiosis and piscirickettsia salmonis in fish: a review. | the bacterium piscirickettsia salmonis is the aetiological agent of piscirickettsiosis a severe disease that has caused major economic losses in the aquaculture industry since its appearance in 1989. recent reports of p. salmonis or p. salmonis-like organisms in new fish hosts and geographical regions have increased interest in the bacterium. because this gram-negative bacterium is still poorly understood, many relevant aspects of its life cycle, virulence and pathogenesis must be investigated b ... | 2014 | 24279295 |
| a functional ferric uptake regulator (fur) protein in the fish pathogen piscirickettsia salmonis. | piscirickettsia salmonis, a gram-negative fastidious facultative intracellular pathogen, is the causative agent of the salmonid rickettsial septicemia (srs). the p. salmonis iron acquisition mechanisms and its molecular regulation are unknown. iron is an essential element for bacterial pathogenesis. typically, genes that encode for the iron acquisition machinery are regulated by the ferric uptake regulator (fur) protein. p. salmonis fur sequence database reveals a diversity of fur genes without ... | 2016 | 27762429 |
| vaccines for piscirickettsiosis (salmonid rickettsial septicaemia, srs): the chile perspective. | piscirickettsia salmonis (p. salmonis) is the aetiological bacterium of the contagious disease piscirickettsiosis or salmonid rickettsial septicaemia (srs) and causes significant economic losses to aquaculture production in chile. current strategies to control infection are i) indiscriminate antibiotic use and ii) vaccination with predominantly p. salmonis bacterin vaccines that do not provide acceptable levels of protection against piscirickettsiosis. areas covered: this review covers the basic ... | 2017 | 27690686 |
| electrochemical detection of piscirickettsia salmonis genomic dna from salmon samples using solid-phase recombinase polymerase amplification. | electrochemical detection of solid-phase isothermal recombinase polymerase amplification (rpa) of piscirickettsia salmonis in salmon genomic dna is reported. the electrochemical biosensor was constructed by surface functionalization of gold electrodes with a thiolated forward primer specific to the genomic region of interest. solid-phase rpa and primer elongation were achieved in the presence of the specific target sequence and biotinylated reverse primers. the formation of the subsequent surfac ... | 2016 | 27220649 |
| resistance-nodulation-division efflux pump acrab is modulated by florfenicol and contributes to drug resistance in the fish pathogen piscirickettsia salmonis. | piscirickettsia salmonis is a fastidious intracellular pathogen responsible for high mortality rates in farmed salmonids, with serious economic consequences for the chilean aquaculture industry. oxytetracycline and florfenicol are the most frequently used antibiotics against p. salmonis, but routine use could contribute to drug resistance. this study identified differentiated florfenicol susceptibilities in two p. salmonis strains, lf-89 and austral-005. the less susceptible isolate, austral-005 ... | 2016 | 27190287 |
| two novel blood-free solid media for the culture of the salmonid pathogen piscirickettsia salmonis. | | 2013 | 23173561 |
| immunostimulatory effects of prolactin on tlr1 and tlr5m in shk-1 cells infected with piscirickettsia salmonis. | the innate immune system is the first line of defense against infection by pathogens. it consists of various elements, including toll-like receptors (tlrs), which recognize molecular patterns associated with pathogens and trigger the immune response, through activation of important transcription factors such as nf-κb, which are usually found sequestered in the cytoplasm by iκbα until it receives the release signal. piscirickettsia salmonis causes piscirickettsiosis or salmonid rickettsial septic ... | 2016 | 27025311 |
| fast detection of piscirickettsia salmonis in salmo salar serum through maldi-tof-ms profiling. | piscirickettsia salmonis is a pathogenic bacteria known as the aetiological agent of the salmonid rickettsial syndrome and causes a high mortality in farmed salmonid fishes. detection of p. salmonis in farmed fishes is based mainly on molecular biology and immunohistochemistry techniques. these techniques are in most of the cases expensive and time consuming. in the search of new alternatives to detect the presence of p. salmonis in salmonid fishes, this work proposed the use of maldi-tof-ms to ... | 2016 | 26956387 |
| characterization and pathogenic role of outer membrane vesicles produced by the fish pathogen piscirickettsia salmonis under in vitro conditions. | piscirickettsia salmonis is one of the major fish pathogens affecting chilean aquaculture. this gram-negative bacterium is highly infectious and is the etiological agent of piscirickettsiosis. little is currently known about how the virulence factors expressed by p. salmonis are delivered to host cells. however, it is known that several gram-negative microorganisms constitutively release outer membrane vesicles (omvs), which have been implicated in the delivery of virulence factors to host cells ... | 2016 | 26854350 |
| comprehensive antibiotic susceptibility profiling of chilean piscirickettsia salmonis field isolates. | antibiotics have been extensively used against infections produced by piscirickettsia salmonis, a fish pathogen and causative agent of piscirickettsiosis and one of the major concerns for the chilean salmon industry. therefore, the emergence of resistant phenotypes is to be expected. with the aim of obtaining a landscape of the antimicrobial resistance of p. salmonis in chile, the susceptibility profiles for quinolones, florfenicol and oxytetracycline (otc) of 292 field isolates derived from mai ... | 2016 | 26660665 |
| identification of the clpb and bipa genes and an evaluation of their expression as related to intracellular survival for the bacterial pathogen piscirickettsia salmonis. | piscirickettsia salmonis is the pathogen responsible for salmonid rickettsial septicemia (srs), a disease that affects a wide variety of marine cultivated fish species and causes economic losses for the aquaculture industry worldwide. many in vitro studies have reported on the capacity of this microorganism to replicate in the interior of cytoplasmic vesicles from varied fish cell lines. however, the mechanisms used by this bacteria to survive, replicate, and propagate in cell lines, especially ... | 2014 | 25205198 |
| diversity of bacteria in ships ballast water as revealed by next generation dna sequencing. | the bacterial diversity in ballast water from five general cargo ships calling at the port of houston was determined with ion semiconductor dna sequencing (ion torrent pgm) of pcr amplified 16s rrna genes. phylogenetic analysis revealed that the composition of bacteria in ballast water did not resemble that of typical marine habitats or even open ocean waters where bwes occur. the predominant group of bacteria in ships conducting bwes was the roseobacter clade within the alphaproteobacteria. in ... | 2016 | 27076378 |
| phenotypic and genetic characterization of piscirickettsia salmonis from chilean and canadian salmonids. | the study presents the phenotypic and genetic characterization of selected p. salmonis isolates from atlantic salmon and rainbow trout suffering from srs (salmonid rickettsial septicemia) in chile and in canada. the phenotypic characterization of the p. salmonis isolates were based on growth on different agar media (including a newly developed medium), different growth temperatures, antibiotics susceptibility and biochemical tests. | 2016 | 26975395 |
| complete genome sequence of piscirickettsia salmonis lf-89 (atcc vr-1361) a major pathogen of farmed salmonid fish. | piscirickettsia salmonis, the causative agent of salmonid rickettsial septicemia (srs), is a significant threat to the healthy and sustainable production of salmonid farming industry. this gram-negative bacterium, originally isolated from a coho salmon in southern chile, produces a systemic infection characterized by colonization of several fish organs. p. salmonis is able to infect, survive, and replicate inside salmonid macrophages however little is known about its mechanisms of pathogenesis. ... | 2015 | 26220311 |
| effectiveness of egg yolk immunoglobulin against the intracellular salmonid pathogen piscirickettsia salmonis. | to produce and characterize egg yolk immunoglobulin (igy) against the fish intracellular pathogen piscirickettsia salmonis as well as to evaluate the antibacterial activity of igy in vitro and the availability in the serum of fish immunized orally. | 2015 | 26018590 |
| patterns of piscirickettsia salmonis load in susceptible and resistant families of salmo salar. | the pathogen piscirickettsia salmonis produces a systemic aggressive infection that involves several organs and tissues in salmonids. in spite of the great economic losses caused by this pathogen in the atlantic salmon (salmo salar) industry, very little is known about the resistance mechanisms of the host to this pathogen. in this paper, for the first time, we aimed to identify the bacterial load in head kidney and muscle of atlantic salmon exhibiting differential familiar mortality. furthermor ... | 2015 | 25862974 |
| the infection process of piscirickettsia salmonis in fish macrophages is dependent upon interaction with host-cell clathrin and actin. | piscirickettsia salmonis is an aggressive fish pathogen that causes piscirickettsiosis, a systemic disease that threatens the sustainability of salmon production in chile. to date, the infection strategies of this bacterium are poorly characterized, a dot/icm type iv secretion system homolog for intracellular multiplication and survival in macrophages is suggested. since an invading pathogen and its host develop a complex interaction in which the pathogen strives to survive and replicate, while ... | 2015 | 25790493 |
| draft genome sequence of virulent strain austral-005 of piscirickettsia salmonis, the etiological agent of piscirickettsiosis. | we report here the draft genome sequence of a lethal pathogen of farmed salmonids, piscirickettsia salmonis strain austral-005. this virulent strain was isolated in 2008 from oncorhynchus mykiss farms, and multiple genes involved in pathogenicity, environmental adaptation, and metabolic pathways were identified. | 2014 | 25323708 |
| infectivity of piscirickettsia salmonis in immersion-bath exposed rainbow trout oncorhynchus mykiss (walbaum) fry. | | 2015 | 25168060 |
| combined effects of high stocking density and piscirickettsia salmonis treatment on the immune system, metabolism and osmoregulatory responses of the sub-antarctic notothenioid fish eleginops maclovinus. | the aim of this study was to evaluate immunological, metabolic and osmoregulatory secondary stress responses in eleginops maclovinus specimens submitted to three different stocking densities: i) low (3.1 kg m(-3)), medium (15 kg m(-3)) and high (60 kg m(-3)) during 10 days, alone or in combination with a previous treatment of a protein extract of the pathogen piscirickettsia salmonis (0.5 μg g weight body(-1)). plasma, liver, gill and kidney samples were obtained at the end of both experiments. ... | 2014 | 25108087 |
| a comparison of neutral and immune genetic variation in atlantic salmon, salmo salar l. in chilean aquaculture facilities. | genetic diversity was assessed in samples of cultured atlantic salmon, salmo salar l., obtained from facilities in chile between 2005 and 2010, a period of time during which the infectious pathogens infectious salmon anemia (isa) virus, caligus rogercresseyi (sea lice), and piscirickettsia salmonis (salmon rickettsial syndrome) were common. two panels of microsatellite markers were utilized: one with microsatellites with no known gene associations (neutral) and one featuring microsatellites link ... | 2014 | 24918941 |
| polymorphism in gyra is associated to quinolones resistance in chilean piscirickettsia salmonis field isolates. | | 2015 | 24917068 |
| disease resistance in atlantic salmon (salmo salar): coinfection of the intracellular bacterial pathogen piscirickettsia salmonis and the sea louse caligus rogercresseyi. | naturally occurring coinfections of pathogens have been reported in salmonids, but their consequences on disease resistance are unclear. we hypothesized that 1) coinfection of caligus rogercresseyi reduces the resistance of atlantic salmon to piscirickettsia salmonis; and 2) coinfection resistance is a heritable trait that does not correlate with resistance to a single infection. | 2014 | 24736323 |
| effect of piscirickettsia salmonis inoculation on the ask continuous cell line. | | 2015 | 24649885 |
| draft genome sequence of the fish pathogen piscirickettsia salmonis. | piscirickettsia salmonis is a gram-negative intracellular fish pathogen that has a significant impact on the salmon industry. here, we report the genome sequence of p. salmonis strain lf-89. this is the first draft genome sequence of p. salmonis, and it reveals interesting attributes, including flagellar genes, despite this bacterium being considered nonmotile. | 2013 | 24201203 |
| broth microdilution protocol for minimum inhibitory concentration (mic) determinations of the intracellular salmonid pathogen piscirickettsia salmonis to florfenicol and oxytetracycline. | | 2014 | 23803014 |
| chemical structure of the carbohydrate backbone of the lipopolysaccharide from piscirickettsia salmonis. | elucidation of the carbohydrate backbone structure of the lipopolysaccharide (lps) from piscirickettsia salmonis, the etiological agent of the salmonid rickettsial septicemia, is described. structural information was established by a combination of monosaccharide and methylation analyses of lps, and by nmr and mass spectrometries of oligosaccharides obtained through the use of various chemical degradations of the native polymer. the following structure of the backbone sugars was determined on th ... | 2013 | 23673236 |
| evidence of exotoxin secretion of piscirickettsia salmonis, the causative agent of piscirickettsiosis. | piscirickettsia salmonis is the aetiological agent of piscirickettsiosis, a disease which affects a variety of teleost species and that is particularly severe in salmonid fish. bacterial-free supernatants, obtained from cultures of three isolates of piscirickettsia salmonis, were inoculated in atlantic salmon, salmo salar l., and in three continuous cell lines in an effort to determine the presence of secretion of extracellular products (ecps) by this microorganism. although steatosis was found ... | 2013 | 23347160 |
| development and preliminary validation of an antibody filtration-assisted single-dilution chemiluminometric immunoassay for potency testing of piscirickettsia salmonis vaccines. | challenge with live pathogens could be substituted by serology for many veterinary diseases, however little progress has been made in the development of alternative batch vaccine potency tests for fish. this study reports the development and preliminary validation of a single-dilution filtration-assisted chemiluminometric immunoassay (sd fal-elisa) applied to measure anti piscirickettsia salmonis igm in individual or pooled serum and mucus samples. the assay was set up to test a single-dilution ... | 2012 | 23040097 |
| biofilm generation by piscirickettsia salmonis under growth stress conditions: a putative in vivo survival/persistence strategy in marine environments. | piscirickettsia salmonis is a bacterial fish pathogen seriously threatening the sustainability of the chilean salmon industry. the biology and life cycle of this bacterium is not completely understood and there are no reports explaining how it survives or persists in marine environments. this work provides descriptive data of p. salmonis behavior when it is exposed to stress conditions, producing large cell aggregates closely resembling typical biofilm structures. in order to track this putative ... | 2012 | 22910282 |
| broth medium for the successful culture of the fish pathogen piscirickettsia salmonis . | piscirickettsiosis or salmonid rickettsial septicaemia (srs) caused by piscirickettsia salmonis constitutes one of the main problems in farmed salmonid and marine fishes. since the first reports of the disease, it has been successfully isolated and maintained in eukaryotic cell--culture systems, but these systems are time-consuming, the media are costly, and eliminating heavily contaminated host cell debris is difficult. in this report, we describe a marine-based broth supplemented with l-cystei ... | 2012 | 22422090 |
| an isolate of piscirickettsia salmonis from white seabass is fully virulent for coho salmon. | the virulence of the wsb-98 isolate of piscirickettsia salmonis from white seabass atractoscion nobilis was compared with that of the american type culture collection type strain lf-89, which was originally isolated from coho salmon oncorhynchus kisutch in chile. in controlled laboratory challenges of juvenile coho salmon, the isolate from white seabass exhibited virulence that was equal to or greater than that of lf-89. the cumulative percent mortality (cpm) was similar between groups of coho s ... | 2006 | 26599161 |
| immunostimulatory effect of salmon prolactin on expression of toll-like receptors in oncorhynchus mykiss infected with piscirickettsia salmonis. | in aquaculture, antibiotics are the traditional treatment used against bacterial infections. however, their use has increasingly come into question given their effects on fish and, possibly, on human health. consequently, there is interest in developing alternative treatments aimed at stimulating the innate immune response of fish, which is the first line of defense against pathogens. in relation to this, the toll-like receptors (tlr) aid in the selective identification of pathogens. the present ... | 2016 | 26537800 |
| genome-wide association analysis reveals loci associated with resistance against piscirickettsia salmonis in two atlantic salmon (salmo salar l.) chromosomes. | pisciricketssia salmonis is the causal agent of salmon rickettsial syndrome (srs), which affects salmon species and causes severe economic losses. selective breeding for disease resistance represents one approach for controlling srs in farmed atlantic salmon. knowledge concerning the architecture of the resistance trait is needed before deciding on the most appropriate approach to enhance artificial selection for p. salmonis resistance in atlantic salmon. the purpose of the study was to dissect ... | 2015 | 26499328 |
| expression of flagellin and key regulatory flagellar genes in the non-motile bacterium piscirickettsia salmonis. | the piscirickettsia salmonis genome was screened to evaluate potential flagella-related open reading frames, as well as their genomic organization and eventual expression. a complete and organized set of flagellar genes was found for p. salmonis, although no structural flagellum has ever been reported for this bacterium. to gain further understanding, the hierarchical flagellar cascade described for legionella pneumophila was used as a reference model for putative analysis in p. salmonis. specif ... | 2017 | 28177291 |
| successive oral immunizations against piscirickettsia salmonis and infectious salmon anemia virus are required to maintain a long-term protection in farmed salmonids. | currently, there is a growing demand to determine the protective status of vaccinated fish in order to prevent diseases outbreaks. a set of different parameters that include the infectious and immunological status of vaccinated salmonids from 622 chilean farms were analyzed during 2011-2014. the aim of this study was to optimize the vaccination program of these centers through the determination of the protective state of vaccinated fish using oral immunizations. this state was determined from th ... | 2015 | 26074916 |
| infectious salmon anaemia virus (isav) in chilean atlantic salmon (salmo salar) aquaculture: emergence of low pathogenic isav-hpr0 and re-emergence of virulent isav-hpr∆: hpr3 and hpr14. | infectious salmon anaemia (isa) is a serious disease of marine-farmed atlantic salmon (salmo salar) caused by isa virus (isav), which belongs to the genus isavirus, family orthomyxoviridae. isa is caused by virulent isav strains with deletions in a highly polymorphic region (hpr) of the hemagglutinin-esterase (he) protein (designated virulent isav-hpr∆). this study shows the historic dynamics of isav-hpr∆ and isav-hpr0 in chile, the genetic relationship among isav-hpr0 reported worldwide and bet ... | 2013 | 24268071 |