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determination of the cyanobacterial toxin cylindrospermopsin in algal food supplements.for the analysis of blue-green algal food supplements for cylindrospermopsin (cyn), a c18 solid-phase extraction column and a polygraphitized carbon solid-phase extraction column in series was an effective procedure for the clean-up of extracts. determination of cyn was by liquid chromatography with ultraviolet light detection. at extract spiking levels of cyn equivalent to 25-500 µg g(-1), blue-green algal supplement recoveries were in the range 70-90%. cyn was not detected in ten samples of fo ...201121623503
adventures with cyanobacteria: a personal perspective.cyanobacteria, or the blue-green algae as they used to be called until 1974, are the oldest oxygenic photosynthesizers. we summarize here adventures with them since the early 1960s. this includes studies on light absorption by cyanobacteria, excitation energy transfer at room temperature down to liquid helium temperature, fluorescence (kinetics as well as spectra) and its relationship to photosynthesis, and afterglow (or thermoluminescence) from them. further, we summarize experiments on their t ...201122645530
phenological and liver antioxidant profiles of adult nile tilapia (oreochromis niloticus) exposed to toxic live cyanobacterium (microcystis aeruginosa kützing) cells.blue-green algae (cyanobacteria) constitute the greater part of the phytoplankton. microcystis aeruginosa is amongst the most ubiquitously distributed cyanobacterial species, and almost invariably produces cyclic heptapeptide toxins called microcystins (mcs). the present study was designed to investigate the phenological and liver antioxidant profiles of the nile tilapia oreochromis niloticus chronically exposed to toxic live m. aeruginosa cells. fish were grown in the absence and presence of m. ...201223413757
biochemical characterization of nadp⁺-dependent isocitrate dehydrogenase from microcystis aeruginosa pcc7806.microcystis aeruginosa is the key symptom of water eutrophication and produces persistent microcystins. our special attention was paid to the isocitrate dehydrogenase (idh) of m. aeruginosa (maidh) because it plays important roles in energy and biosynthesis metabolisms and its catalytic product 2-oxoglutarate provides the carbon skeleton for ammonium assimilation and also constitutes a signaling molecule of nitrogen starvation in cyanobacteria. sequence alignment showed that maidh shared signifi ...201223264072
the veterinarian's part in the regulation of the movement of livestock and meat. 20154675709
Complex microbiome underlying secondary and primary metabolism in the tunicate-Prochloron symbiosis.The relationship between tunicates and the uncultivated cyanobacterium Prochloron didemni has long provided a model symbiosis. P. didemni is required for survival of animals such as Lissoclinum patella and also makes secondary metabolites of pharmaceutical interest. Here, we present the metagenomes, chemistry, and microbiomes of four related L. patella tunicate samples from a wide geographical range of the tropical Pacific. The remarkably similar P. didemni genomes are the most complex so far as ...201122123943
A Fungus Capable of Degrading Microcystin-LR in the Algal Culture of Microcystis aeruginosa PCC7806.Microcystins (MCs) are a family of natural toxins produced by cyanobacteria (blue-green algae). Microbial degradation is considered an efficient method for eliminating cyanobacteria and MCs in environmental conditions. This study examines the ability of Trichaptum abietinum 1302BG, a white rot fungus, to degrade microcystin-LR in the harmful algal culture of Microcystis aeruginosa PCC7806. Results showed that microcystin-LR could not be detected by high-performance liquid chromatography after 12 ...201122167691
acute exposure to microcystin-producing cyanobacterium microcystis aeruginosa alters adult zebrafish (danio rerio) swimming performance parameters.microcystins (mcs) are toxins produced by cyanobacteria (blue-green algae), primarily microcystis aeruginosa, forming water blooms worldwide. when an organism is exposed to environmental perturbations, alterations in normal behavioral patterns occur. behavioral repertoire represents the consequence of a diversity of physiological and biochemical alterations. in this study, we assessed behavioral patterns and whole-body cortisol levels of adult zebrafish (danio rerio) exposed to cell culture of t ...201122253623
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