Publications

sequence-specific endonucleases from the cyanobacterium nostoc sp. atcc 29132.	the nitrogen-fixing cyanobacterium nostoc sp. atcc 29132 was shown to contain two sequence-specific endonucleases. nsp(29132) i was an isoschizomer of asuii, and nsp(29132) ii was an isoschizomer of bamhi. nsp(29132) ii was shown to generate ends that could be ligated to those generated by bamhi.	1991	1840140
gene cluster rpobc1c2 in cyanobacteria does not constitute an operon.	the core enzyme of the cyanobacterial dna-dependent rna polymerase contains a unique component, gamma, which is absent from the corresponding enzymes of other eubacteria. in the heterocystous cyanobacterium nostoc commune the gene encoding gamma, rpoc1, is immediately adjacent to, and downstream of, rpob. the rpoc1 gene, and a 3' adjacent gene, rpoc2, correspond to the single rpoc gene found in escherichia coli with respect to those domains conserved within their translational products. northern ...	1991	1899176
cadmium toxicity to photosynthesis and associated electron transport system of nostoc linckia.		1991	1900451
growth, chlorophyll a content, nitrogen-fixing ability, and certain metabolic activities of nostoc muscorum: effect of methylparathion and benthiocarb.		1991	1932863
physiological sources of reductant for nitrogen fixation activity in nostoc sp. strain ucd 7801 in symbiotic association with anthoceros punctatus.	pure cultures of the symbiotic cyanobacterium-bryophyte association with anthoceros punctatus were reconstituted by using nostoc sp. strain ucd 7801 or its 3-(3,4-dichlorophenol)-1,1-dimethylurea (dcmu)-resistant mutant strain, ucd 218. the cultures were grown under high light intensity with co2 as the sole carbon source and then incubated in the dark to deplete endogenous reductant pools before measurements of nitrogenase activities (acetylene reduction). high rates of light-dependent acetylene ...	1991	1938924
binding of cadmium by cyanobacterial growth media: free ion concentration as a toxicity index to the cyanobacterium nostoc uam 208.	a quantitative study of cadmium binding to three different growth media for nitrogen-fixing cyanobacteria was done with the aid of a solid state ion-specific electrode. kratz and myers modified medium and arnon medium bound large amounts of cd2+, bg11o medium had less binding capacity. of the media components, phosphate ion showed the greatest ability to bind cd2+. different phs, the size of cell inoculum and two buffers (tricine and hepes, 25 mm) also changed the availability of free cadmium io ...	1991	1953030
orientation and linear dichroism of mastigocladus laminosus phycocyanin trimer and nostoc sp. phycocyanin dodecamer in stretched poly(vinyl alcohol) films.	the linear dichroism (ld) spectra of the c-phycocyanin (c-pc) trimer disks oriented in poly(vinyl alcohol) films (pva) at room temperature and at 95 k were determined. utilizing the known atomic coordinates of the chromophores (schirmer, t., bode, w. and huber, r. (1987) j. mol. biol. 196, 677-695) and theoretical estimates of the orientations of the transition dipole moments relative to the molecular framework, the ld spectra were simulated using the pairwise exciton interaction model of sauer ...	1991	1905956
the amino-acid sequence of three proteins of photosystem i of the cyanobacterium fremyella diplosiphon (calothrix sp pcc 7601).	three proteins containing 138 amino acids (psad protein), 80 amino acids (psac protein) and 66 amino acids (psae protein) of the photosystem i (ps i) complex of the cyanobacterium fremyella diplosiphon (calothrix sp pcc 7601) were isolated and sequenced. comparison with previously known sequences showed a close relationship to homologous proteins of nostoc, another filamentous cyanobacterium.	1991	1930733
influence of different forms of nitrogen on uptake of ammonium, glutamate and glutamine in the cyanobacterium nostoc muscorum.	effect of various types of nitrogen nutrition was studied on the uptake of ammonium, glutamate and glutamine by nostoc muscorum and its het-nif- mutant. ammonium nitrogen acted as a potent inhibitor/repressor of ammonium, glutamate and glutamine transport. nitrate nitrogen was found to be a strong inhibitor/repressor of ammonium transport, a partial inhibitor/repressor of glutamate transport but, caused a partial stimulation of glutamine transport.	1991	1684351
evidence for a role of glutamine synthetase in assimilation of amino acids as nitrogen source in the cyanobacterium nostoc muscorum.	methylammonium/ammonium ion, glutamine, glutamate, arginine and proline uptake, and their assimilation as nitrogen sources, was studied in nostoc muscorum and its glutamine synthetase-deficient mutant. glutamine served as nitrogen source independent of glutamine synthetase activity. glutamate was not metabolised as a nitrogen source but still inhibited nitrogenase activity and diazotrophic growth. glutamine synthetase activity was essential for the assimilation of n2, ammonia, arginine and proli ...	1991	1687107
evidence for energy-dependent copper efflux as a mechanism of cu2+ resistance in the cyanobacterium nostoc calcicola.	wild-type nostoc calcicola carried out oxygenic photosynthesis extremely sensitive to copper. a cu(2+)-resistant mutant (cu-r1) of the cyanobacterium grew normally at high concentrations of cu2+. its ability to grow under such conditions was found to be due to mutational acquisition of an energy-dependent efficient system of cu(2+)-efflux, which rendered cu(2+)-inhibited oxygenic photosynthesis fully reversible.	1991	1804762
effect of lead on nitrogenase and enzymes of nitrogen assimilation in a cyanobacterium nostoc muscorum.	lead decreased the growth rates, total cell mass, heterocyst frequency, total cell protein, nitrogenase activity, glutamine synthetase (gs) and glutamate synthase (gogat) activities in n:muscorum. however, lead at 0.01 and 10 micrograms ml-1 conc. enhanced nitrogenase as well as gs activity of the cells. on transfer to excess lead (100 micrograms ml-1), nitrogenase and gs activities ceased almost after 24 hr in the cyanobacterium. it is deduced that lead has a two step effect on stimulation and ...	1991	1816095
[production of the antibiotic-algicide cyanobacterin lu-2 by a filamentous cyanobacterium nostoc sp].	a strain of cyanobacterium of nostoc has been isolated, and found to produce a new antibiotic cyanobacterin lu-2. the antibiotic is synthesized by the cyanobnacterium under intensive cultivation conditions in a liquid mineral medium. cyanobacterin lu-2 is an exometabolite; its maximum accumulation in the medium is achieved at 34 degrees. cyanobacterin lu-2 is active against many cyanobacteria tested, including those of microcystis and aphanizomenon which are principals to give rise to blooms in ...	1991	1819038
response of enzymes involved in the processes of antioxidation towards benthiocarb and methylparathion in cyanobacteria nostoc muscorum.		1991	1912703
psad is required for the stable binding of psac to the photosystem i core protein of synechococcus sp. pcc 6301.	the psac gene product from synechococcus sp. pcc 7002 and the psad gene product from nostoc sp. pcc 8009 were synthesized in escherichia coli and purified to homogeneity. incubation of the psac apoprotein with the synechoccus sp. pcc 6301 photosystem i core protein in the presence of fecl3, na2s, and beta-mercaptoethanol resulted in a time-dependent transition in the flash-induced absorption change from a 1.2-ms, p700+ fx- back-reaction to a long-lived, p700+ [fa/fb]- back-reaction. esr studies ...	1991	1651109
elucidation and optimization of the medium constituents controlling antibiotic production by the cyanobacterium nostoc muscorum.	a study has been made to determine which nutrient factors control antibiotic production by the cyanobacterium, nostoc muscorum. a two-phase approach was employed using a factorial method to explore the response surface and a steepest ascent method to climb the response surface to the region of the optimum. it was found that nitrate and iron were the factors significantly affecting antibiotic production; 26.4 mm nitrate and 6 microm iron were the optimal concentrations for maximizing antibiotic p ...	1991	1367529
[2 site-specific endonucleases of the cyanobacterium nostoc linckia].	two restrictases nli387/7 i and nli387/7 ii have been isolated from cyanobacterium nostoc linckia using chromatography on phosphocellulose, "mono q" column, and heparin sepharose 4b. the preparations are described by the method of electrophoresis in polyacrylamide gel under denaturing conditions. catalytic properties of restrictases are determined: optimal ph of the action--9.0--9.5, optimal concentration of na+--5 mm, that of mg2+--6 mm, optimal temperature--37 degrees c. the isolated enzymes a ...	1991	1650423
toxic effects of omega chrome red me and its treatment by adsorption.	toxic effects of omega chrome red me, a popular textile dye, on the nitrogen fixing cyanobacterium nostoc calcicola were studied. the growth of n. calcicola was found to be suppressed at 10 and 20 mg liter-1 initial concentrations of dye, whereas a low initial concentration of 5 mg liter-1 slightly favors growth. removal of the dye was carried out by adsorption using some cheap and unconventional adsorbents like coal, fly ash, wollastonite, and china clay. it has been observed that, in all cases ...	1992	1280581
absorption of color additives and settling volume in water of blue-green alga, ishikurage (nostoc commune).	the properties of ishikurage (nostoc commune) and other algae were compared. ishikurage and suizenji nori (aphanothece sacrum) absorbed much more amaranth than others. they have a high settling volume (sv) in water. the high sv of ishikurage is attributable to its neutral detergent fiber (ndf). the acid detergent fiber (adf) from the alga had a high amaranth absorption. however the absorption by the adf did not explain the absorption by ishikurage. the results suggest that ishikurage may have a ...	1992	1312238
protective effects of ca2+, mg2+, cu2+, and ni2+ on mercury and methylmercury toxicity to a cyanobacterium.	toxicological investigations of the impact of inorganic mercury (hg2+) and methylmercury (ch3hg+) in terms of growth, nh4+ uptake, in vivo glutamine synthetase (transferase) activity, and regulation of toxicity by ca2+, mg2+, cu2+ and ni2+ in the diazotrophic cyanobacterium nostoc calcicola bréb. have been completed. photoautotrophic growth of the cyanobacterium was extremely sensitive to both mercury compounds, ch3hg+ being 2.5 times more toxic than hg2+. although nh4+ uptake was 6 times more s ...	1992	1375141
abscisic acid and its synthetic analog in relation to growth and nitrogenase activity of azotobacter chroococcum and nostoc muscorum.	the plant hormone abscisic acid as well as its synthetic analog lab 173711 significantly increased the nitrogenase activity of the bacterium azotobacter chroococcum and the cyanobacterium nostoc muscorum. the effect depended on the concentration of the substances (0.001-10 mg/l) and the age of the cultures. the biomass of the organisms was not significantly influenced.	1992	1387101
three new microcystins, cyclic heptapeptide hepatotoxins, from nostoc sp. strain 152.	three new cyclic heptapeptide hepatotoxins, [d-ser1,admadda5]microcystin-lr (1), [d-asp3,-admadda5]microcystin-lhar (2), and [admadda5,mser7]microcystin-lr (3), were isolated from the cyanobacterium (blue-green alga) nostoc sp. strain 152, together with four known microcystins, [admadda5]microcystin-lr (4), [admadda5]microcystin-lhar (5), [d-asp3,-admadda5]microcystin-lr (6), and [dmadda5]microcystin-lr (7). the structures of new microcystins were assigned on the basis of high-resolution fast at ...	1992	1391612
nucleotide sequence of the replication region of the nostoc pcc 7524 plasmid pdu1.		1992	1408769
cyanobiont diversity within and among cycads of one field site.	limited diversity was found among cyanobionts from a cultivated population of cycads at a field site in florida. all isolates were classified as nostoc but were different from the one nostoc species found in the soil. these cyanobacteria were root endophytes of several plants of zamia integrifolia and one of dioon. the isolates were similar morphologically and in their reactions to four fluorescein isothiocyanate conjugated lectins. electrophoretic protein profiles and zymograms distinguished on ...	1992	1288849
molecular cloning and sequencing of the beta-isopropylmalate dehydrogenase gene from the cyanobacterium spirulina platensis.	the gene for beta-isopropylmalate dehydrogenase (ec 1.1.1.85) of spirulina platensis (leub) was cloned from a lambda embl3 genomic library by heterologous hybridization using the nostoc ucd 7801 leub gene as a probe. the sequence of the entire leub coding region was determined as well as 645 bp of 5' flanking region and 956 bp of 3' flanking region. dna sequencing revealed an open reading frame of 1065 nucleotides capable of encoding a polypeptide of 355 amino acids. homologies between the amino ...	1992	1593261
myoglobin in a cyanobacterium.	myoglobin was found in the nitrogen-fixing cyanobacterium nostoc commune. this cyanobacterial myoglobin, referred to as cyanoglobin, was shown to be a soluble hemoprotein of 12.5 kilodaltons with an amino acid sequence that is related to that of myoglobins from two lower eukaryotes, the ciliated protozoa paramecium caudatum and tetrahymena pyriformis. cyanoglobin is encoded by the glbn gene, which is positioned between nifu and nifh-two genes essential for nitrogen fixation-in the genome of nost ...	1992	1609281
construction of insertion mutants of synechocystis sp. pcc 6803: evidence for an essential function of subunit iv of the cytochrome b6/f complex.	the gene encoding subunit iv of the cytochrome b6/f complex (petd) has been isolated from a genomic library of the unicellular cyanobacterium synechocystis sp. pcc 6803. the coding region consists of 480 nucleotides and can code for a polypeptide with a molecular weight of 17.5 kda. the deduced amino acid sequence shows high identity with the corresponding sequences of both the photoautotrophic prokaryote nostoc sp. pcc 7906 as well as of lower and higher photoautotrophic eukaryotes (e.g. chlore ...	1992	1590707
identification of a second psac gene in the cyanobacterium synechocystis sp. pcc6803.	the psac gene encodes the 9 kda protein subunit of photosystem i that carries the iron-sulfur centers fa and fb. the paper describes the sequence and transcription analysis of a second psac gene (psac2) from synechocystis sp. pcc6803. thepsac2 gene is transcribed into an abundant monocistronic mrna. the deduced amino acid sequence of psi-c2 is very similar to the protein sequences from two other cyanobacteria (synechococcus sp. pcc7002 and nostoc sp. pcc8009). in contrast, the recently isolated ...	1992	1463835
differential effect of ultraviolet-b radiation on certain metabolic processes in a chromatically adapting nostoc.	the impact of uv-b radiation on growth, pigmentation and certain physiological processes has been studied in a n2-fixing chromatically adapting cyanobacterium, nostoc spongiaeforme. a brownish form (phycoerythrin rich) was found to be more tolerant to uv-b than the blue-green (phycocyanin rich) form of n. spongiaeforme. continuous exposure to uv-b (5.5 w m-2) for 90 min caused complete killing of the blue-green strain whereas the brown strain showed complete loss of survival after 180 min. pigme ...	1992	1561238
differentiation of free-living anabaena and nostoc cyanobacteria on the basis of fatty acid composition.	the cellular fatty acids of free-living, nitrogen-fixing cyanobacteria belonging to the genera anabaena and nostoc were analyzed to differentiate the genera. the fatty acid compositions of 10 anabaena strains and 10 nostoc strains that were grown for 12 days on bg-11o medium were determined by gas-liquid chromatography-mass spectroscopy. of the 53 fatty acids detected, 17 were major components; the average level for each of these 17 fatty acids was at least 0.9% of the total fatty acids (in at l ...	1992	1581185
cloning and sequencing of the petbd operon from the cyanobacterium synechococcus sp. pcc 7002.	the genes encoding the photosynthetic cytochrome b6 (petb) and subunit 4 (petd) have been cloned and sequenced from the unicellular, photoheterotrophic, transformable cyanobacterium synechococcus sp. pcc 7002, formerly designated agmenellum quadruplicatum. the gene arrangement was found to be similar to that reported in the cyanobacterium nostoc pcc 7906. the dna and derived protein sequences were compared to chloroplast and the other cyanobacterial sequences. by pulsed-field electrophoresis, th ...	1992	1421151
semipreparative isolation of individual cyanobacterial heterocyst-type glycolipids by reverse-phase high-performance liquid chromatography.	procedures are described for the quantitative, semi-preparative isolation of individual cyanobacterial heterocyst-type glycolipids (hgs) by reverse-phase hplc (rp-hplc) and the modifications to conventional techniques necessary to prevent significant hg losses during sample preparation. total lipids are obtained from cultures of nitrogen-fixing cyanobacteria by triplicate extraction with 200 packed-cell volumes of chloroform/methanol (1/1, v/v), filtered, and redissolved in chloroform for loadin ...	1992	1443590
methods involving light variation for isolation of cyanobacteria: characterization of isolates from central australia.	we report the isolation of organisms belonging to a range of cyanobacterial genera from the remote arid region of central australia, together with a preliminary characterization of their temporal modes of nitrogen fixation. we rendered unialgal dermocarpa and myxosarcina spp. (section ii organisms), lpp group b: type x (section iii), and scytonema and nostoc spp. (section iv). we developed an isolation procedure based on a combination of published methods and applicable to a broad spectrum of cy ...	1992	16348803
the rubisco activase (rca) gene is located downstream from rbcs in anabaena sp. strain ca and is detected in other anabaena/nostoc strains.	a gene encoding ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) activase (rca) was found downstream from the rbclrbcs operon in the heterocystous cyanobacterium anabaena sp. strain ca. two unknown open reading frames were shown to be located between rbcs and rca in strain ca and all the genes, rbclrbcs, orf1, orf2, and rca were in the same transcriptional orientation. the deduced amino acid sequence of the anabaena rubisco activase showed both similarities and differences to the plant ...	1993	8467074
copper toxicity and phosphate utilization in the cyanobacterium nostoc calcicola.		1993	8422521
characterization of genes for an alternative nitrogenase in the cyanobacterium anabaena variabilis.	anabaena variabilis atcc 29413 is a heterotrophic, nitrogen-fixing cyanobacterium that has been reported to fix nitrogen and reduce acetylene to ethane in the absence of molybdenum. dna from this strain hybridized well at low stringency to the nitrogenase 2 (vnfdgk) genes of azotobacter vinelandii. the hybridizing region was cloned from a lambda embl3 genomic library of a. variabilis, mapped, and sequenced. the deduced amino acid sequences of the vnfd and vnfk genes of a. variabilis showed only ...	1993	8407800
the patb gene product, required for growth of the cyanobacterium anabaena sp. strain pcc 7120 under nitrogen-limiting conditions, contains ferredoxin and helix-turn-helix domains.	a mutant of anabaena sp. strain pcc 7120, called pat-2, that grows poorly under nitrogen-fixing conditions, has been isolated. the heterocysts of the mutant strain develop much more slowly than those of the wild type and are spaced more closely in an older culture of the mutant than in the wild type. the wild-type gene that complements the mutation in pat-2, called patb, was isolated and characterized. the predicted 529-amino-acid patb protein contains a region very similar to the fe4s4 bacteria ...	1993	8449877
recovery of nostoc muscorum previously exposed to some triazine and phenylurea herbicides.		1993	8490271
multisensory activation of the phosphorelay initiating sporulation in bacillus subtilis: identification and sequence of the protein kinase of the alternate pathway.	the phosphorelay is the signal-transduction system recognizing and integrating environmental signals to initiate sporulation. the major signal input to the phosphorelay is an atp-dependent kinase, kina, responsible for phosphorylating the spoof protein. mutants lacking kina, however, still sporulate, suggesting that other kinases can fulfil its role. in order to identify these kinases, genes for kinases were isolated by hybridization using a degenerate oligonucleotide probe designed for common r ...	1993	8497199
characterization of a restriction barrier and electrotransformation of the cyanobacterium nostoc pcc 7121.	we have investigated host restriction as a barrier to transformation and developed a method for gene transfer into the previously untransformable, heterotrophic cyanobacterium nostoc pcc 7121. a restriction endonuclease, designated nsp 7121i, has been partially purified by phosphocellulose chromatography of nostoc cell extracts. comparisons of nsp 7121i digests of bacteriophage lambda and plasmid dnas with computer-generated restriction fragment profiles showed that nsp 7121i is an isoschizomer ...	1993	8215799
rehydration induces rapid onset of lipid biosynthesis in desiccated nostoc commune (cyanobacteria).	water, which contained [1,3-3h]glycerol, [35s]sodium sulfate, or [32p]sodium orthophosphate, was used to rehydrate air-dried cells of the desiccation-tolerant filamentous cyanobacterium nostoc commune. the cells retained their capacities for the uptake and transport of all three compounds and, in response to rewetting, they mobilized the radiolabels into lipid precursors and initiated complex lipid biosynthesis. the onset of these events, measured in short-term, long-term and pulse-chase labelin ...	1993	8504158
an osmotic stress protein of cyanobacteria is immunologically related to plant dehydrins.	dehydrins are a family of desiccation proteins that were identified originally in plants (t.j. close, a.a. kortt, p.m. chandler [1989] plant mol biol 13: 95-108; g. galau, t.j. close [1992] plant physiol 98: 1523-1525). dehydrins are characterized by the consensus amino acid sequence domain ekkgimdkikeklpg found at or near the carboxy terminus; the core of this domain (kikeklpg) may be repeated from one to many times within the complete polypeptide. dehydrins generally accumulate in plants in re ...	1993	8310057
a gene encoding a protein related to eukaryotic protein kinases from the filamentous heterocystous cyanobacterium anabaena pcc 7120.	protein kinases play essential roles in the development of eukaryotic cells. these enzymes display various degrees of sequence similarity in their catalytic domains. this conservation has allowed the identification of protein kinases in a variety of organisms, including the gram-negative bacterium myxococcus xanthus. in this study, sequences related to those encoding eukaryotic protein kinases were amplified by pcr from dna of anabaena pcc 7120, a filamentous cyanobacterium that differentiates c ...	1993	7505448
a protein-tyrosine/serine phosphatase encoded by the genome of the cyanobacterium nostoc commune utex 584.	protein-tyrosine phosphorylation has long been regarded as an exclusively eukaryotic phenomenon. although some non-eukaryotes, mainly viruses, possess genes encoding protein-tyrosine kinases or protein-tyrosine phosphatases, these were probably appropriated from the eukaryotic hosts that constitute the sites of action of these enzymes. herein we identify a gene, iphp, from the chromosome of the cyanobacterium nostoc commune utex 584 that contains the his-cys-xaa-ala-gly-xaa-xaa-arg sequence char ...	1993	7681825
a small plasmid, pca2.4, from the cyanobacterium synechocystis sp. strain pcc 6803 encodes a rep protein and replicates by a rolling circle mechanism.	different cryptic plasmids are widely distributed in many strains of cyanobacteria. a small cryptic plasmid, pca2.4, from synechocystis strain pcc 6803 was completely sequenced, and its replication mode was determined. pca2.4 contained 2,378 bp and encoded a replication (rep) protein, designated repa. an analysis of the deduced amino acid sequence revealed that repa of pca2.4 has significant homology with rep proteins of pkym from shigella sonnei, a pub110 plasmid family from gram-positive bacte ...	1993	8320214
mutational replacement of molybdenum by vanadium in assimilation of n2 or no3- as nitrogen source in the cyanobacterium nostoc muscorum.	tungsten resistant (wr) mutants of het+nif+nia+, het+nif-nia+ and het+nif+nia- strains of nostoc muscorum were isolated with severely defective molybdate transport activity. all such mutants showed vanadium (v)-dependent nitrogenase activity and/or nitrate reductase activity and v-dependent growth on n2-nitrogen and/or no3(-)-nitrogen and v-dependent no3(-)-repression of heterocyst formation and nitrogenase activity. none of them grew with molybdenum (mo) under parallel growth condition. results ...	1993	8330016
plasmids from two morphologically distinct cyanobacterial strains share a novel replication origin.	a 2.9-kbp replication origin from a plasmid endogenous to the filamentous cyanobacterium fremyella diplosiphon utex 481 was genetically characterized and sequenced. deletion analysis of the 2.9-kbp dna fragment delimited the minimum region necessary for replication in f. diplosiphon fd33 to approximately 2.5 kbp. dna sequence analysis revealed that the f. diplosiphon plasmid replication origin is structurally very similar to and shares significant identity with the 1.75-kbp replication origin re ...	1993	8366056
general distribution of the nitrogen control gene ntca in cyanobacteria.	the ntca gene from synechococcus sp. strain pcc 7942 encodes a regulatory protein which is required for the expression of all of the genes known to be subject to repression by ammonium in that cyanobacterium. homologs to ntca have now been cloned by hybridization from the cyanobacteria synechocystis sp. strain pcc 6803 and anabaena sp. strain pcc 7120. sequence analysis has shown that these ntca genes would encode polypeptides strongly similar (77 to 79% identity) to the synechococcus ntca prote ...	1993	8366058
cloning and expression of the nspv restriction-modification genes of nostoc sp. strain pcc7524.		1993	8367311
characterization of a cta/cde operon-like genomic region encoding subunits i-iii of the cytochrome c oxidase of the cyanobacterium synechocystis pcc 6803.	strong heterologous hybridization of a synthetic oligonucleotide probe of 17 bp originally used to clone subunit i of the paracoccus denitrificans cytochrome c oxidase (m. raitio, t. jalli and m. saraste (1987) embo j. 6, 2825-2833) to a single band was observed on southern blots of anacystis nidulans r2 (synechococcus pcc 7942), synechocystis pcc 6803, and nostoc mac pcc 8002 chromosomal dna digests. six pooled gene banks prepared from synechocystis pcc 6803 contained regions that hybridized to ...	1993	8387368
cadmium mediated control of nitrogenase activity and other enzymes in a nitrogen fixing cyanobacterium.	cadmium decreased the specific growth rate and total cell mass at concentrations > 0.001 micrograms ml-1. it also reduced heterocyst frequency, total cell protein, nitrogenase activity (acetylene reduction) and decreased glutamine synthetase (gs) and glutamate synthase (gogat) activities. however, cells grown under low concentration of cadmium (0.001 microgram ml-1) had enhanced nitrogenase as well as gs activity besides showing increased growth rate and heterocyst frequency. cadmium has apparen ...	1994	7866728
cloning and nucleotide sequence of the gene encoding dinitrogenase reductase (nifh) from the cyanobacterium nostoc 6720.	the nucleotide sequence of the 3' end of the nifu coding sequence, the complete coding sequence of nifh and a substantial part of the 5' end of nifd coding sequence from nostoc 6720 is presented. the coding sequences are highly conserved with those of anabaena 7120 and anabaena sp. l31. however the intergenic region between nifu and nifh contains two segments of short tandemly repetitive repeat sequences (strrs) that differ from the strr that is common to both anabaena7120 and anabaenasp. l31. v ...	1994	7918657
sequence of a 2.6-kb cryptic plasmid from a marine cyanobacterium synechococcus sp.	we have shown previously that the copy number of plasmid psy10 from the marine cyanobacterium synechococcus sp. nkbg 042902 is dependent on the salinity of the growth medium. we report here the complete nucleotide sequence (2561 bp) of this plasmid. the longest open reading frame, orf-b (1.08 kb), occurs on a 1.6-kb ecori fragment. this orf encodes a putative protein which is 360 aa residues in length and is 37.8% homologous to the replication protein of plasmid pca2.4 from synechocystis sp. str ...	1994	7899510
biochemical effects of carbaryl on nitrogen assimilating enzymes of cyanobacteria nostoc muscorum.		1994	7912573
the gene encoding cytochrome-c oxidase subunit i from synechocystis pcc6803.	the gene (coxi or coxa) encoding subunit i (coi) of cytochrome-c oxidase (cytochrome aa3) of synechocystis pcc6803, synechococcus pcc7942 (anacystis nidulans r2) and nostoc pcc8002 (nostoc mac), was identified by heterologous hybridization of chromosomal digests with a 17-bp oligodeoxyribonucleotide (probe c) derived from the coxi of paracoccus denitrificans. a single genomic fragment was found to bind to probe c in all chromosomal digests. due to its favorable signal-to-noise ratio, the genome ...	1994	8125290
structure, composition, and assembly of paracrystalline phycobiliproteins in synechocystis sp. strain bo 8402 and of phycobilisomes in the derivative strain bo 9201.	the phycobiliproteins of the unicellular cyanobacterium synechocystis sp. strain bo 8402 and its derivative strain bo 9201 are compared. the biliproteins of strain bo 8402 are organized in paracrystalline inclusion bodies showing an intense autofluorescence in vivo. these protein-pigment aggregates have been isolated. the highly purified complexes contain phycocyanin with traces of phycoerythrin, corresponding linker polypeptides lr35pc and lr33pe (the latter in a small amount), and a unique col ...	1994	8300542
hypocholesterolemic effect of blue-green alga, ishikurage (nostoc commune) in rats fed atherogenic diet.	blue-green alga, nostoc commune is rich in dietary fiber. to examine the hypocholesterolemic effect, the alga or the dietary fibers were given to the rats fed cholesterol diet. serum cholesterol elevation was significantly depressed in those rats. cholesterol increased in the liver of rats given the alga and the fiber. the content of fecal cholesterol and bile acid unchanged. of the dietary fibers, oxalate-oxalic acid soluble substance (ooss) found abundant in the alga was most effective in lowe ...	1994	8146104
nuclear genes encoding chloroplast hemoglobins in the unicellular green alga chlamydomonas eugametos.	when the green unicellular alga chlamydomonas eugametos is grown under light/dark regimes, nuclear genes are periodically activated in response to the changes in light conditions. these genetic responses are dependent upon the activation of genes associated with photosynthesis (li616 and li637), nonphotosynthetic photoreceptors (li410 and li818) and the biological clock (li818). we report here that the li410 and li637 genes are part of a small gene family encoding hemoglobins (hbs) related to th ...	1994	8177215
common nitrogen control of caesium uptake, caesium toxicity and ammonium (methylammonium) uptake in the cyanobacterium nostoc muscorum.	studies were carried out to examine the role of ammonium transport activity in the control of caesium uptake and toxicity in nostoc muscorum. the results showed a definite specific role of the ammonium-repressible/derepressible ammonium transport system of the cyanobacterium in caesium uptake, accumulation and toxicity. furthermore, the results showed that n. muscorum can acquire resistance against diazotrophically-associated caesium toxicity when supplied with ammonium as a nitrogen source. in ...	1994	8200501
energetics of cyanophage n-1 multiplication in the diazotrophic cyanobacterium nostoc muscorum.	cyanophage n-1 multiplication was investigated during the latent period of the virus, when 14co2 fixation was inhibited whereas respiratory o2 uptake increased approximately 67% at 4 h after infection. a simultaneous decrease (70%) in the glycogen content of infected cells indicated its catabolic involvement. a chloramphenicol-sensitive rise in glucose-6-phosphate dehydrogenase activity as a result of n-1 infection partly explained the increase in aerobic respiration. the total atp pool declined ...	1994	8078415
transposon mutagenesis of nostoc sp. strain atcc 29133, a filamentous cyanobacterium with multiple cellular differentiation alternatives.	nostoc sp. strain atcc 29133 (pcc 73102; nostoc 29133) is a symbiotically-competent, facultatively heterotrophic, diazotrophic cyanobacterium with the capacity to differentiate specialized cells such as heterocysts, akinetes and hormogonial filaments. we have optimized several methods for physiological and molecular genetic analysis of nostoc 29133. by use of a tn5 derivative, tn5-1063 (km(r)bm(r)sm(r)), delivered by conjugation from escherichia coli, antibiotic-resistant mutants of nostoc 29133 ...	1994	7881544
detection and characterization of cyanobacterial nifh genes.	the dna sequence of a 359-bp fragment of nifh was determined for the heterocystous strains anabaena sp. strain ca (atcc 33047), nostoc muscorum utex 1933, a nostoc sp., gloeothece sp. strain atcc 27152, lyngbya lagerheimii utex 1930, and plectonema boryanum iu 594. results confirmed that the dna sequence of the 359-bp segment is sufficiently variable to distinguish cyanobacterial nifh genes from other eubacterial and arachaeobacterial nifh genes, as well as to distinguish heterocystous from nonh ...	1994	8161180
desiccation tolerance of prokaryotes.	the removal of cell-bound water through air drying and the addition of water to air-dried cells are forces that have played a pivotal role in the evolution of the prokaryotes. in bacterial cells that have been subjected to air drying, the evaporation of free cytoplasmic water (vf) can be instantaneous, and an equilibrium between cell-bound water (vb) and the environmental water (vapor) potential (psi wv) may be achieved rapidly. in the air-dried state some bacteria survive only for seconds where ...	1994	7854254
cryptophycin: a new antimicrotubule agent active against drug-resistant cells.	cryptophycin is a cytotoxic dioxadiazacyclohexadecenetetrone isolated from cyanobacteria of the genus nostoc. incubation of l1210 leukemia cells with cryptophycin resulted in dose-dependent inhibition of cell proliferation in parallel with increases in the percentage of cells in mitosis (half-maximal effects at < 10 pm). indirect immunofluorescence studies demonstrated that treatment of a-10 vascular smooth muscle cells with cryptophycin results in marked depletion of cellular microtubules and r ...	1994	7913408
two mutations that block heterocyst differentiation have different effects on akinete differentiation in nostoc ellipsosporum.	evident differentiation of vegetative cells into heterocysts in anabaena sp. strain pcc 7120 is prevented by insertions in genes hetr and hetp. nostoc ellipsosporum possesses single copies of genes that hybridize with hetr and hetp. in mutant ne2 of n. ellipsosporum, in which hetr is interrupted by an insert, and in a double recombinant of wild-type n. ellipsosporum with a plasmid that bears an interrupted copy of hetr, neither heterocysts nor akinetes are formed. when an intact copy of hetr fro ...	1994	7934891
mutational analysis of the nh4+-nitrogen controls that regulate ammonium transport activity, heterocyst differentiation, nitrogenase activity and the heterocyst-spacing pattern in the cyanobacterium nostoc muscorum.	mutational analysis of the genetic determinants mediating nh(4+)-nitrogen regulating effects on nh(4+)-transport activity, heterocyst differentiation, nitrogenase activity and heterocyst pattern formation was carried out in nostoc muscorum. evidence suggested the operation of three separate genetic determinants in such nitrogen control; one mediating nh(4+)-repression control on both heterocyst formation and nh(4+)-transport activity, a second (nif-r) mediating nh(4+)-repression control on nitro ...	1994	8019441
analysis of the sequences within and flanking the cyanoglobin-encoding gene, glbn, of the cyanobacterium nostoc commune utex 584.	a 3.5-kb segment of dna containing nifu glbn nifh nifd was cloned from a gene library of nostoc 584 and sequenced. the nifu-glbn intergenic region contains short tandemly repeated repetitive sequences (5'-aattacg). a sequence corresponding to a nifa-like upstream activator sequence (with the consensus recognition sequence for bifa in anabaena 7120), elements of a nifh promoter and a sequence that may function as a transcription terminator, were identified downstream from glbn. glbn, unique to ce ...	1994	8063099
isolation and characterization of temperature-sensitive mutants of cyanophage n-1.	optimal conditions for the induction of temperature-sensitive (ts) mutants of cyanophage n-1 were established after mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine (mnng). a treatment with mnng (400 micrograms/ml) for 2 hrs at ph 8.0 induced ts-mutants at a maximum frequency of 1.46 x 10(-3). a characterization of 10 such ts-mutants with regard to adsorption, one-step growth and temperature-shift experiments with nostoc muscorum as host bacterium led to the identification of temperature-se ...	1994	8067307
athermal physiological effects of microwaves on a cynobacterium nostoc muscorum: evidence for em-memory bits in water.	athermal physiological effects of continuous wave and modulated microwaves were studied on a cynobacterium nostoc muscorum. the study shows that different microwave frequencies in continuous wave and modulated modes produced significantly different physiological effects on the algae. water-mediated bioeffects further present additional proof that water has the capability to remember the imposed electromagnetic field characteristics for an extended period of time.	1994	8022214
assessment of the effect of the toxicity of a textile dye on nostoc muscorum isu, a diazotrophic cyanobacterium.	the impact of graded concentrations (5, 10, 15 and 20 mg dm(-3) of acid mordant metomega chrome orange gl, a common dye used in woollen, carpet and textile industries, was studied on protein and pigment content and photosynthetic oxygen evolution in a diazotrophic cyanobacterium nostoc muscorum. the lower concentration of 5 mg dm(-3) showed a negligible effect on protein and pigment content and photosynthetic oxygen evolution, whereas at higher concentrations a drastic decrease in the above para ...	1994	15091720
water stress proteins of nostoc commune (cyanobacteria) are secreted with uv-a/b-absorbing pigments and associate with 1,4-beta-d-xylanxylanohydrolase activity.	acidic water stress polypeptides (wsp) with molecular masses of 33, 37 and 39 kda are the most abundant soluble proteins in the cyanobacterium nostoc commune. wsp polypeptides and uv-a/b-absorbing pigments are secreted by cells, accumulate in the extracellular glycan sheath, and are released from desiccated colonies upon rehydration. no evidence was obtained for either glycosylation, phosphorylation, or acylation of wsp polypeptides. nh2-terminal amino acid sequences of the 33-, 37-, and 39-kda ...	1994	8125999
factors regulating methyl mercury uptake in a cyanobacterium.	the simultaneous addition of dithiothreitol (dtt), mercaptoethanol, and glutathione (30 microm each) and ch3hg+ to nostoc calcicola cells reduced ch3hg+ uptake in the order gsh > dtt > mercaptoethanol. however, the preexposure of cyanobacterial cells to similar thiols resulted in different pattern of ch3hg+ uptake in the sequence: gsh > mercaptoethanol > dtt. light-grown cyanobacterial cells demonstrated a faster initial uptake of ch3hg+ (rate 0.619 mumol ch3hg+ mg-1 protein min-1, 10 min) with ...	1995	8565882
disintegration of phycobilisomes in a rice field cyanobacterium nostoc sp. following uv irradiation.	the effects of ultraviolet-b (uv-b, 280-315 nm) irradiation on survival, growth and phycobilisome assembly have been studied in the n2-fixing cyanobacterium nostoc sp. survival and growth were virtually arrested after 120 min of uv irradiation. the phycobiliprotein fractions showed a decrease in absorption and fluorescence as well as a shift towards shorter wavelengths indicating the impairment of energy transfer from phycobiliproteins to the photosynthetic reaction centers. this was further sup ...	1995	8589643
plasmid and chromosomal dna recovery by electroextraction of cyanobacteria.	high voltage electroporation has been investigated as a method for rapid recovery of plasmid and chromosomal dna from the cyanobacteria nostoc pcc 7121, synechococcus pcc 7002, and anabaena pcc 7120. pulses of 18 kv/cm and higher applied to concentrated nostoc cells carrying a shuttle plasmid (prl25) resulted in copious release of nucleic acids and phycobiliproteins into the suspending medium. small portions of these supernatants, when electroporated with escherichia coli, gave rise to hundreds ...	1995	7781980
characterization of ts-mutants of cyanophage n-1 by their inactivation by physical and chemical agents.	the effect of temperature, ultraviolet (uv) light and ethylenediaminetetraacetic acid (edta) on the stability of cyanophage n-1, infecting the cyanobacterium nostoc muscorum was studied. complete inactivation of the phage occurred at 60 degrees c in 6 mins. all the temperature-sensitive (ts) mutants exhibited faster inactivation at 50 degrees c than the wild type. uv light readily inactivated the particles of the wild giving a survival of 3.44% at a dose of 60 secs. all the ts-mutants were found ...	1995	7676938
structure of a novel oligosaccharide-mycosporine-amino acid ultraviolet a/b sunscreen pigment from the terrestrial cyanobacterium nostoc commune.	water-soluble uv-a/b-absorbing pigments are secreted by cells of the cosmopolitan terrestrial cyanobacterium nostoc commune. the pigments constitute a complex mixture of monomers with molecular masses of up to 1801 da. two different chromophores with absorption maxima at 312 and 335 nm are linked to different amino acids and to oligosaccharides consisting of galactose, glucose, xylose, glucuronic acid, and glucosamine. the 335 nm chromophore is a 1,3-diaminocyclohexen derivative, while the chrom ...	1995	7721752
growth and some physiological aspects of nostoc muscorum in response to mixtures of two triazine herbicides.		1995	7742636
structure and evolution of paramecium hemoglobin genes.	hemoglobin (hb) genes have been cloned from three different species of ciliated protists, p. multimicronucleatum, p. triaurelia and p. jenningsi. southern blotting of the genomic dnas using the p. caudatum hb cdna showed both intraspecies variation in different stocks of p. caudatum and interspecies variation within the genus paramecium. the isolated hb genes were composed of 118, 117 and 117 codons, and interrupted by a short intron with 27, 29 and 29 bp at the same position, in p. multimicronu ...	1995	7578257
characterization of toxin-producing cyanobacteria by using an oligonucleotide probe containing a tandemly repeated heptamer.	cyanobacteria produce toxins that kill animals. the two main classes of cyanobacterial toxins are cyclic peptides that cause liver damage and alkaloids that block nerve transmission. many toxin-producing strains from finnish lakes were brought into axenic culture, and their toxins were characterized. restriction fragment length polymorphism analysis, probing with a short tandemly repeated dna sequence found at many locations in the chromosome of anabaena sp. strain pcc 7120, distinguishes hepato ...	1995	7592362
genetic evidence of a major role for glucose-6-phosphate dehydrogenase in nitrogen fixation and dark growth of the cyanobacterium nostoc sp. strain atcc 29133.	heterocysts, sites of nitrogen fixation in certain filamentous cyanobacteria, are limited to a heterotrophic metabolism, rather than the photoautotrophic metabolism characteristic of cyanobacterial vegetative cells. the metabolic route of carbon catabolism in the supply of reductant to nitrogenase and for respiratory electron transport in heterocysts is unresolved. the gene (zwf) encoding glucose-6-phosphate dehydrogenase (g6pd), the initial enzyme of the oxidative pentose phosphate pathway, was ...	1995	7592384
multiple metal resistance in the cyanobacterium nostoc muscorum.		1995	7767039
nucleotide sequence of an operon in nostoc sp. strain atcc 29133 encoding four genes of the oxidative pentose phosphate cycle.		1995	7870816
two heterocyst-specific dna rearrangements of nif operons in anabaena cylindrica and nostoc sp. strain mac.	two site-specific dna rearrangements occur during heterocyst differentiation in the cyanobacterium anabaena sp. strain pcc 7120: the deletion of an 11 kb element from within the nifd gene and the deletion of a 55 kb element from within the fdxn gene. three nostoc and six anabaena strains were screened for the presence of the nifd and fdxn elements by southern hybridization with anabaena pcc 7120 dna probes. eight of the nine strains contained dna sequences that were similar to the nifd element. ...	1995	7582007
interaction of cryptophycin 1 with tubulin and microtubules.	the cryptophycins are newly discovered antimitotic agents isolated from the cyanobacterium nostoc. previous studies using cultured cells demonstrated that microtubules are the target of these compounds. we have studied the interaction of cryptophycin 1 with tubulin and microtubules in vitro. cryptophycin 1 is an effective inhibitor of tubulin polymerization, causes tubulin to aggregate, and depolymerizes microtubules to linear polymers somewhat similar to the spiral-like structures produced by t ...	1995	8543019
promiscuity of heme groups in the cyanobacterial cytochrome-c oxidase.	the cyanobacteria nostoc sp. strain mac, anabaena 7937, synechocystis 6803, and anacystis nidulans (synechococcus 6301) were grown and incubated in the light under three different oxygen regimes: phase-a cells were harvested from photoautotrophically growing cultures at a cell density of 2.8-3.2 microliter packed cell mass/ml and an oxygen concentration of approx. 350 microm (corresponding to > 150% air saturation). phase-b cells were harvested 24 hrs after 20 microm 3-(3,4-dichlorophyl)-1,1-dim ...	1995	8747548
effective concentrations of nitrophenolics toward growth yield of selected microalgae and cyanobacteria isolated from soil.	the effective concentrations (ec), which cause a certain percentage of inhibition in growth yield, of four nitrophenolics (o-nitrophenol, m-nitrophenol, p-nitrophenol (pnp), and 2,4-dinitrophenol) and the major metabolites of pnp reduction (p-nitrosophenol and p-aminophenol) toward growth of two microalgae (chlorella vulgaris and scenedesmus bijugatus) and two cyanobacteria (nostoc muscorum and nostoc linckia), all isolated from soil, were determined following linear regression analysis. the ec5 ...	1995	8964245
heterocyst formation.	heterocysts are microaerobic, n2-fixing cells that form in a patterned array within o2-producing filamentous cyanobacteria. structural features of heterocysts can be predicted from consideration of their physiology. this review focuses on the spacing mechanism that determines which cells will differentiate, and on the regulation of the progression of the differentiation process. applicable genetic tools, developed primarily using anabaena pcc 7120, but employed also with nostoc spp., are reviewe ...	1996	8982449
purification and spectroscopic characterization of a recombinant chloroplastic hemoglobin from the green unicellular alga chlamydomonas eugametos.	hemoglobins (hb), which have the important task of delivering molecular oxygen by facilitating its reversible binding to the heme, are now thought to have evolved in all groups of organisms including prokaryotes, fungi, plants and animals. our recent finding of a light-inducible chloroplastic hb in the green unicellular alga chlamydomonas eugametos has further extend this idea, while raising questions about the function that an hb could play in a high oxygen environment such as in the chloroplas ...	1996	9022709
cyclic peptides and depsipeptides from cyanobacteria: a review.	an elaborate array of structurally-novel and biologically-active cyclic peptides and depsipeptides are found in blue-green algae (cyanobacteria). several of these compounds possess structures that are similar to those of natural products from marine invertebrates. most of these cyclic peptides and depsipeptides, such as the microcystins and the lyngbyatoxins, will probably only be useful as biochemical research tools. a few, however, have the potential for development into useful commercial prod ...	1996	8730577
glbn (cyanoglobin) is a peripheral membrane protein that is restricted to certain nostoc spp.	the glbn gene of nostoc commune utex 584 is juxtaposed to nifu and nifh, and it encodes a 12-kda monomeric hemoglobin that binds oxygen with high affinity. in n. commune utex 584, maximum accumulation of glbn occurred in both the heterocysts and vegetative cells of nitrogen-fixing cultures when the rate of oxygen evolution was repressed to less than 25 micromol of o2 mg of chlorophyll a(-1) h(-1). accumulation of glbn coincided with maximum synthesis of nifh and ferredoxin nadp+ oxidoreductase ( ...	1996	8932316
transcriptional regulation of zwf, encoding glucose-6-phosphate dehydrogenase, from the cyanobacterium nostoc punctiforme strain atcc 29133.	the gene encoding glucose-6-phosphate dehydrogenase (g6pd), zwf, in nostoc punctiforme strain atcc 29133 is part of a four-gene operon that also encodes fructose bisphosphatase (fbp), transaldolase (tal) and a gene product termed opca, which is contranscribed with zwf and essential for g6pd activity. the effect of exogenous nitrogen and carbon sources on transcription of these genes was investigated. growth in the presence of ammonium yielded low levels of transcripts encoding all genes of the o ...	1996	8939431
spectroscopical and functional characterization of the hemoglobin of nostoc commune (utex 584 (cyanobacterial).	structural analysis of a monomeric hemoglobin from the cyanobacterium nostoc commune strain utex 584, cyanoglobin (potts et al. (1992) science 256, 1690-1692), is presented. cyanoglobin binds molecular oxygen reversibly, with high oxygen affinity and non-cooperativity. there was no evidence for decreased stability of the pigment at 37 degrees c. cyanoglobin-specific antibodies showed no cross-reactivity with two reference hemoglobins, leghemoglobin a and sperm whale myoglobin. the absorption spe ...	1996	8547335
impact of aluminium, fluoride and fluoroaluminate complex on atpase activity of nostoc linckia and chlorella vulgaris.	this study demonstrates a ph-dependent inhibition of mg(2+)- and ca(2+)-atpase activities of nostoc linckia and chlorella vulgaris exposed to alcl3, alf3, naf and alcl3+naf together. alf3 and the combination of alcl3+naf were more inhibitory to both the enzymes as compared with alcl3 and naf. toxicity of the test compounds increased with increasing acidity. interaction of alcl3+naf was additive on n. linckia and c. vulgaris, respectively, at ph 7.5 and 6.8, and synergistic at ph 6.0 and 4.5. in ...	1996	8696079
the devr gene product is characteristic of receivers of two-component regulatory systems and is essential for heterocyst development in the filamentous cyanobacterium nostoc sp. strain atcc 29133.	strain ucd 311 is a transposon-induced mutant of nostoc sp. strain atc c 29133 that is unable to fix nitrogen in air but does so under anoxic conditions and is able to establish a functional symbiotic association with the hornwort anthoceros punctatus. these properties of strain ucd 311 are consistent with previous observations that protection against oxygen inactivation of nitrogenase is physiologically provided within a. punctatus tissue. upon deprivation of combined nitrogen, strain ucd 311 c ...	1996	8606181
ca2+ uptake and its regulation in the cyanobacterium nostoc mac	the uptake of ca2+ and its regulation in the cyanobacterium nostoc mac were investigated. cation uptake pattern was found to be biphasic, consisting of (a) rapid binding of cations to the negatively charged cell surface and (b) its metabolism dependent on intracellular import at least up to 60 min with the saturation at 2 mm ca2+ (km, 1.5 mm, vmax 42.1 nmol ca2+ mg-1 protein min-1 ). the cellular ca2+ uptake was light and atp dependent, and the addition of 3(3,4-dichlorophenyl)-1,1-dimethyl urea ...	1996	8661678
transaldolase genes from the cyanobacteria anabaena variabilis and synechocystis sp. pcc 6803: comparison with other eubacterial and eukaryotic homologues.	we have sequenced and analysed the transaldolase (tal) genes from two cyanobacteria, anabaena variabilis (atcc 29413) and synechocystis sp. pcc 6803, which are filamentous heterocyst-forming and unicellular organisms, respectively. the deduced amino acid sequences of the two cyanobacterial tal genes are 78% identical and are highly homologous to both eubacterial and eukaryotic transaldolases (escherichia coli, two yeasts, and man) with values ranging from 54 to 60% amino acid identity. in contra ...	1996	8616240
geosiphon pyriforme, a fungus forming endocytobiosis with nostoc (cyanobacteria), is an ancestral member of the glomales: evidence by ssu rrna analysis.	geosiphon pyriforme inhabiting the surface of humid soils represents the only known example of endocytobiosis between a fungus (zygomycotina; macrosymbiont) and cyanobacteria (nostoc; endosymbiont). in order to elucidate the taxonomical and evolutionary relationship of geosiphon pyriforme to fungi forming arbuscular mycorrhiza (am fungi), the small-subunit (ssu) ribosomal rna genes of geosiphon pyriforme and glomus versiforme (glomales; a typical am fungus) were analyzed and aligned with ssu rrn ...	1996	8660431
reconstitution of the 2fe-2s center and g = 1.89 electron paramagnetic resonance signal into overproduced nostoc sp. pcc 7906 rieske protein.	the rieske 2fe-2s protein is a distinguishing subunit of the photosynthetic electron transport cytochrome b6f complex in chloroplast and cyanobacterial thylakoid membranes. we have constructed plasmids for overproduction in escherichia coli of fusion, full-length, and truncated forms of the rieske (petc) protein from the cyanobacterium nostoc sp. pcc 7906. a glutathione s-transferase/rieske fusion protein was used to prepare specific chicken egg-yolk antibodies against the rieske protein. expres ...	1996	8952502
regulation of photosynthetic enzymes in light- or dark-grown cyanobacterium nostoc sp. mac.		1996	8878946
effect of cadmium on the bioelement composition of nostoc uam208: interaction with calcium.		1997	9060371