Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the potassium channel opening action of pinacidil; studies using biochemical, ion flux and microelectrode techniques. | in rat aorta and rat portal vein, (-)- and (+)-pinacidil each produced a concentration-dependent inhibition of tension development. although the (-) isomer was the more potent, concentration effect curves for each isomer were steep with similar slopes. in rat portal vein, tetraethylammonium and procaine antagonised the relaxant effect of (+/-)-pinacidil, whereas 3,4-diamino-pyridine was without effect. intracellular microelectrode recording in rat portal vein showed that low concentrations of (+ ... | 1988 | 2848206 |
| the role of diffusible molecules in regulating the cellular differentiation of dictyostelium discoideum. | a central problem in developmental biology is to understand how morphogenetic fields are created and how they act to direct regionalized cellular differentiation. this goal is being pursued in organisms as diverse as moulds, worms, flies, frogs and mice. each organism has evolved its own solution to the challenge of multicellularity but there appear to be common underlying principles and, once pattern formation is fully understood in any system, some general truths seem certain to be revealed. a ... | 1988 | 2848678 |
| effects of gestational exposure to phencyclidine: distribution and neurochemical alterations in maternal and fetal brain. | phencyclidine is a widely used drug of abuse and is known to produce a wide variety of psychoactive effects. pcp abuse by pregnant women has been reported to result in the birth of infants exhibiting irritability, jitteriness and hyperactivity with high pitched cries. the present study was designed to evaluate the distribution of pcp in the maternal and fetal brain and the neurochemical effects produced by gestational exposure. pregnant sprague-dawley rats were treated sc with 5 mg/kg pcp on 3 c ... | 1989 | 2576301 |
| proton nmr and cd solution conformation determination and opioid receptor binding studies of a dynorphin a(1-17) model peptide. | the opioid peptide dynorphin a(1-17) contains a peptide segment in residues 7-15 with the potential to form an amphiphilic beta-strand. this amphiphilic structure may, like the amphiphilic alpha-helices found in many other peptide hormones, be an important determinant of its interactions with membranes and receptors. in order to investigate and characterize these interactions, we have synthesized a 17-residue dynorphin analogue (yggflkkvkpkvkvkss) that incorporates a peptide model of this amphip ... | 1989 | 2574597 |
| effect of prolonged infusion of anf in normotensive and hypertensive monkeys. | it is now recognized that bolus and short-term infusions of atrial natriuretic factor (anf) into different species lead to a slight and transient decrease of blood pressure, while prolonged infusions cause a significant blood pressure reduction in hypertensive but not in normotensive rats. the present study was designed to evaluate the effects of prolonged anf infusions on blood pressure and humoral parameters in normotensive and hypertensive african green monkeys (cercopithecus aethiops). human ... | 1989 | 2553071 |
| effects of irradiation on diabetes in the bb/wor rat. | lymphoid irradiation is known to prevent spontaneous autoimmune diabetes in susceptible bb rats. the present studies investigated further the effects of radiation in diabetes prone (dp) and resistant (dr) bb/wor rats, and histocompatible yoshida (yos) rats. single doses of total body gamma irradiation (125-600 rads) induced diabetes within 22-44 days in 20 of 102 (20%) 30 day old dr rats, less than 1% of which develop the disease. radiation was also associated with (1) a reduction in the ratio o ... | 1989 | 2491639 |
| [experimental evolutive cycle of diphyllobothrium erinaceieuropei in paracyclops fimbriatus, tadpoles of bufo arenarum and dogs]. | experiments were performed in order to develop the life cycle of diphyllobothrium erinaceieuropei rudolphi 1819 (cestoda, pseudophyllidea) in paracyclops fimbriatus and bufo arenarum as intermediate hosts and dogs as definitive hosts. the eggs of diphyllobothrium erinaceieuropei from faeces of naturally infected dogs were kept refrigered, in water. in order to obtain coracidiums they were incubated at 25 degrees c, and then were placed in a flask which contained paracyclops fimbriatus. the copep ... | 1989 | 2516918 |
| cough associated with canine vaccination? | 1989 | 2588459 | |
| [prospects for measuring the rheologic characteristics of human soft tissues based on the recording of their transverse rigidity]. | it is shown by an example of a forearm that to determine objective characteristics of rheological properties of soft human tissues by measuring their transverse stiffness (k) one should first investigate the relation between k and the diameter of the used indentor (d). the nonlinearity of the dependence k(d) for indentor diameters from 2 to 8 mm is demonstrated and it is assumed that this nonlinearity is due to heterogeneity of tissue properties in the directions normal to the axis of force acti ... | 1989 | 2765581 |
| use of synthetic ribosome binding site for overproduction of the 5b protein of insertion sequence is5. | insertion sequence is5 is a bacterial transposable element which contains three open reading frames designated 5a, 5b and 5c. although there was no detectable expression from the 5b open reading frame when it was preceded by the native promoter and ribosome binding site or by a tac promoter and the native ribosome binding site, we have overproduced a 5b protein both in vitro and in escherichia coli cells by using a tac promoter and a specially-designed synthetic ribosome binding site. beta-galac ... | 1989 | 2538799 |
| the nature and function of polypeptide growth factor receptors in the human placenta. | 1989 | 2561287 | |
| the surface electrocardiogram as a means of refining a murine pressure overload model of congestive heart failure. | 1989 | 2590929 | |
| pulsed er:yag- and 308 nm uv-excimer laser: an in vitro and in vivo study of skin-ablative effects. | using a pulsed xecl excimer laser (308 nm) and a pulsed er:yag laser (2,940 nm), we investigated skin ablation as a function of pulse number, radiant energy, and repetition rate. in vitro analysis of lesions performed in freshly excised human skin were consistent with in vivo results obtained from experiments on pig skin. pulsed 308 nm laser radiation caused considerable nonspecific thermal tissue injury followed by an inflammatory reaction and impaired healing of lesions in vivo. these findings ... | 1989 | 2716457 |
| the quantitation by radioimmunoassay of 2'-deoxyuridine 5'-triphosphate in extracts of thymidylate synthase-inhibited cells. | a radioimmunoassay (ria) for dutp, with a sensitivity of 3.78 fmol, has been developed. the antibody cross-reacted with dttp so that affinity purification of the immunoglobulin g fraction was required before its use in the ria. cross-reactivity with utp and with mono- and diphosphodeoxyuridylates has necessitated respectively sodium periodate oxidation and anion exchange chromatography of cell extracts, prior to ria quantitation of dutp directly in fractions from the chromatography column. mean ... | 1989 | 2729554 |
| type iii machado-joseph disease in a japanese family: a clinicopathological study with special reference to the peripheral nervous system. | the clinical and pathological findings in a 58-year-old japanese man suffering from type iii machado-joseph disease are reported. the patient became affected at the age of 50 years and presented cerebellar ataxia, progressive external ophthalmoplegia and muscular atrophy, although extrapyramidal signs were never detected throughout the whole course of his disease. his mother, sister and son presented progressive ataxia in the third or fourth decade. the mode of inheritance is considered to be au ... | 1989 | 2743650 |
| a fluorescence study of substrate and inhibitor binding to bovine liver dihydrofolate reductase. | 1. covalent coupling of fluorescein to methotrexate (mtx) by a 5-carbon spacer yields a dihydrofolate reductase (dhfr) inhibitor (fmtx) with ki = 11 nm. 2. fmtx shows a fluorescence quenching with respect to fluorescein which is relieved by binding to the enzyme. 3. the dissociation constants (kd) of mtx, fmtx, nadph and 7,8-dihydrofolate (dhf) from bovine liver dhfr have been determined by fluorometric titrations. 4. the kd values for nadph, mtx and fmtx from the complementary binary complexes ... | 1989 | 2744204 |
| the surface electrocardiogram as a means of refining a murine pressure overload model of congestive heart failure. | 1989 | 2590929 | |
| comparison of a 550,000 dalton cartilage matrix glycoprotein in cartilage from immature and mature dogs. | cartilage matrix glycoprotein is a disulfide bonded 550,000 dalton protein found in cartilage and in the vitreous of the eye. immunofluorescence studies using a specific antiserum to this glycoprotein have previously demonstrated a difference between the topographic distribution of staining for cartilage matrix glycoprotein in cartilage from immature and mature animals. using a polyclonal antiserum to cartilage matrix glycoprotein, we studied this protein in cartilage from normal immature and ma ... | 1989 | 2666656 |
| isolation of a high frequency donor of rhodobacter capsulatus by integration of the plasmid pmt1000 into the chromosome. | the temperature-independent clone of rhodobacter capsulatus ua7041, carrying the temperature-sensitive plasmid pmt1000, has been obtained by selection for plasmid markers at the non-permissive temperature. the transfer to escherichia coli of all drug resistance encoded by pmt1000 was of about 10(-7) when the donor was the ua7041 strain, and of 5 x 10(-4) when the donor was the temperature-sensitive strain of r. capsulatus. electrophoretic analyses showed no plasmid band typical of the autonomous ... | 1989 | 2679770 |
| safrole, eugenol and methyleugenol induce intrachromosomal recombination in yeast. | deletion of an integrated plasmid, a specific type of intrachromosomal recombination, was evaluated for inducibility with the phenylpropenes safrole, eugenol and methyleugenol in the yeast saccharomyces cerevisiae. these phenylpropenes are found in food products, spices, pharmaceuticals and clove cigarettes. safrole and eugenol are known carcinogens in animals and methyleugenol is a suspected carcinogen. these phenylpropenes are not detectable by the ames assay and most other short-term tests us ... | 1989 | 2685589 |
| safrole, eugenol and methyleugenol induce intrachromosomal recombination in yeast. | deletion of an integrated plasmid, a specific type of intrachromosomal recombination, was evaluated for inducibility with the phenylpropenes safrole, eugenol and methyleugenol in the yeast saccharomyces cerevisiae. these phenylpropenes are found in food products, spices, pharmaceuticals and clove cigarettes. safrole and eugenol are known carcinogens in animals and methyleugenol is a suspected carcinogen. these phenylpropenes are not detectable by the ames assay and most other short-term tests us ... | 1989 | 2685589 |
| the paramyxoviridae: aspects of molecular structure, pathogenesis, and immunity. | 1989 | 2690597 | |
| demonstration of axonal branching of fibres from certain precerebellar nuclei to the cerebellar cortex and nuclei: a retrograde fluorescent double-labelling study in the cat. | the projections from certain brain stem precerebellar nuclei to the cerebellar cortex and nuclei have been examined in the cat by using the retrograde fluorescent double-labelling technique. crystalline fluoro-gold was implanted into the left cerebellar nuclei from the contralateral side and rhodamine-b-isothiocyanate was injected into the overlying cerebellar cortex. the inferior olive, the lateral reticular nucleus, and the reticular tegmental pontine nucleus all contained double- as well as s ... | 1989 | 2707349 |
| demonstration of axonal branching of fibres from certain precerebellar nuclei to the cerebellar cortex and nuclei: a retrograde fluorescent double-labelling study in the cat. | the projections from certain brain stem precerebellar nuclei to the cerebellar cortex and nuclei have been examined in the cat by using the retrograde fluorescent double-labelling technique. crystalline fluoro-gold was implanted into the left cerebellar nuclei from the contralateral side and rhodamine-b-isothiocyanate was injected into the overlying cerebellar cortex. the inferior olive, the lateral reticular nucleus, and the reticular tegmental pontine nucleus all contained double- as well as s ... | 1989 | 2707349 |
| almitrine bismesylate reduces hypoxic pulmonary vasoconstriction in isolated rat lungs. | the purpose of this study is to test how almitrine bismesylate (alm) affects the function of pulmonary vasculature during normoxic ventilation, and whether low doses of alm not causing detectable vasoconstriction during normoxic ventilation potentiate hypoxic pulmonary vasoconstriction (hpvc). isolated wistar male rat lungs were perfused with homologous blood at constant flow, and venous and ventilatory pressure. in the first experiment, after equilibration, dose-response curves to alm (from 0 t ... | 1989 | 2711381 |
| carrageenan-induced pulmonary emphysema of rabbit. | a transbronchial injection of 0.75% carrageenan in physiologic saline induced pneumonia followed by emphysema in the insulted lobe. in the stages of pneumonia, scattered infiltration of polymorphonuclear leukocytes was seen throughout the affected lobe within a few days of treatment; later this was replaced by the accumulation of carrageenan-laden macrophages, which lasted for one to two months. enlargement of alveoli and alveolar ducts appeared 2 weeks to 2 months after the treatment, and pulmo ... | 1989 | 2711383 |
| decreased 3 alpha-androstanediol glucuronide levels in plasma and random urines in male pseudohermaphroditism caused by 5 alpha-reductase deficiency. | concentrations of 3 alpha-diol glucuronide (3 alpha-diol g) in plasma and/or random urine samples were determined in seven subjects with familial male pseudohermaphroditism (fmp) due to 5 alpha-reductase deficiency (5 alpha-rd). all subjects were natives of an isolated turkish village with a high incidence of consanguineous marriage. a specific and sensitive antibody to 3 alpha-diol was used for radioimmunoassay of 3 alpha-diol g after hydrolysis and chromatographic purification. the mean plasma ... | 1989 | 2770532 |
| time-resolved extrinsic fluorescence of aromatic-l-amino-acid decarboxylase. | the coenzyme-linked fluorescence of aromatic-l-amino-acid decarboxylase decays non-exponentially. the decay of both native and nabh4 reduced samples can only be fitted by two exponentials each roughly accounting for about half of the total fluorescence. denaturation of the reduced protein with 8 m urea makes the fluorescence decay mono-exponential, like that observed for the reference compound pyridoxamine-5-phosphate. an extra pyridoxyl moiety can be bound to the enzyme after incubation with ex ... | 1989 | 2752045 |
| selective infection of human cd4+ cells by simian immunodeficiency virus: productive infection associated with envelope glycoprotein-induced fusion. | simian immunodeficiency virus (siv) and human immunodeficiency virus share the property of tropism for cd4-bearing cells. infection is initiated by a high-affinity interaction between cd4 and conserved domains on the viral envelope glycoprotein. in this report, we demonstrated that siv had a restricted host range among human cd4+ cells when compared with human immunodeficiency virus type 1 or type 2. this restricted tropism was associated with the inability of the siv envelope glycoprotein to in ... | 1989 | 2784571 |
| effect of cck antagonists cr 1409 and cr 1505 on rat pancreatic exocrine secretion in vivo. | the action of cholecystokinin (cck) antagonists cr 1409 and cr 1505 on pancreatic exocrine secretion stimulated by exogenous and endogenous cck was studied in vivo in anesthetized rats, and compared with proglumide. intravenous administration of cr 1409 and cr 1505 in graded doses between 0.04 and 25 mg/kg/h resulted in a dose-dependent inhibition in pancreatic juice volume and amylase output stimulated by intravenous infusion of cck-8 in a dose of 0.06 microgram/kg/h. cr 1409 is 1,000 times and ... | 1989 | 2813333 |
| effect of cck antagonists cr 1409 and cr 1505 on rat pancreatic exocrine secretion in vivo. | the action of cholecystokinin (cck) antagonists cr 1409 and cr 1505 on pancreatic exocrine secretion stimulated by exogenous and endogenous cck was studied in vivo in anesthetized rats, and compared with proglumide. intravenous administration of cr 1409 and cr 1505 in graded doses between 0.04 and 25 mg/kg/h resulted in a dose-dependent inhibition in pancreatic juice volume and amylase output stimulated by intravenous infusion of cck-8 in a dose of 0.06 microgram/kg/h. cr 1409 is 1,000 times and ... | 1989 | 2813333 |
| impaired oxygenation of gastric mucosa in portal hypertension. the basis for increased susceptibility to injury. | increased susceptibility to mucosal damage is a prominent feature of portal hypertensive gastropathy. since the portal hypertensive gastric mucosa has extensive microvascular changes, we postulated that the increased sensitivity to mucosal damage could have an ischemic basis. we measured distribution of gastric serosal and mucosal oxygenation in a group of portal hypertensive and sham-operated rats, and then studied the effects of intragastric aspirin. in the basal state, gastric mucosa of porta ... | 1989 | 2914542 |
| lecithin: cholesterol acyltransferase activity in patients with chronic liver diseases and positive lp-x tests. | lecithin: cholesterol acyltransferase activity has been measured in serum from patients with various liver disorders and positive lp-x tests and from healthy subjects. statistical analysis indicated that lecithin: cholesterol acyltransferase activity provided no help in the discrimination between the persons of both groups although the mean activities differed significantly. | 1989 | 210070 |
| thermodynamics of binding of oxidized and reduced nicotinamide adenine dinucleotides, adenosine-5'-diphosphoribose, and 5'-iodosalicylate to dehydrogenases. | 1990 | 208598 | |
| comparison of in vitro biological properties and mouse toxicities of three thymidine analogs active against human immunodeficiency virus. | three analogs of thymidine, d4t [2',3'-didehydro-2',3'-dideoxythymidine; 1-(2,3-dideoxy-beta-d-glyceropent-2-enofuranosyl)thymine], fddt (3'-fluoro-3'-deoxythymidine), and azt (3'-azido-3'-deoxythymidine), were compared in biological tests designed to assess their potential utility as anti-human immunodeficiency virus (hiv) agents. the in vitro potencies of these compounds against hiv infection in cem cells were measured, with fddt and azt being more potent than d4t. the cytotoxicities of d4t, f ... | 1990 | 1693057 |
| treatment of behavioral disorders in animals. | 1990 | 2386258 | |
| [immunohistochemical study and lectin distribution of thyroid carcinoma originated from follicular epithelium]. | 97 cases of thyroid carcinoma originated from follicular epithelium were investigated by using histological and immunohistochemical techniques with special reference to lectin distribution. according to the who histological typing of thyroid tumours, these cases were divided into three categories as follows: papillary carcinoma of thyroid (pct) 56, follicular carcinoma of thyroid (fct) 31 and undifferentiated carcinoma of thyroid (uct) 10. results showed that three different kinds of thyroid car ... | 1990 | 2390796 |
| vestibular papillae of the vulva. lack of evidence for human papillomavirus etiology. | vestibular papillae of the vulva are usually considered as anatomical variants of the vestibular mucosa. clinically, however, they are quite often interpreted as condylomata acuminata and recent studies have suggested that they could be related to human papillomavirus infection. this prompted us to search for human papillomavirus dna using the southern blot hybridization technique, by analyzing biopsy specimens taken from 29 patients who presented with papillae of the vestibular mucosa. human pa ... | 1990 | 2175164 |
| studies on the mechanism of oxidative phosphorylation. positive cooperativity in atp synthesis. | kinetic and nucleotide binding studies have shown that submitochondrial particles from bovine heart possess three exchangeable binding sites for adp or gdp. in order of decreasing affinity at neutral ph, these sites will be referred to as sites i, ii, and iii, and their respective dissociation constants as ki, kii, and kiii. in oxidative phosphorylation experiments in the presence of saturating amounts of inorganic phosphate, rapid atp (or gtp) synthesis occurred only upon adp (or gdp) binding t ... | 1990 | 2294123 |
| glucocorticoid stimulation of fatty acid synthesis in explants of human fetal lung. | we examined the effects of glucocorticoids and thyroid hormone (t3) on fatty acid synthesis, fatty acid composition and fatty acid synthetase activity in explants of human fetal lung (16-23 wk gestation). explants were cultured 1-7 days in the absence (control) or presence of dexamethasone (10 nm) and/or t3 (2 nm). in control explants fatty acid synthesis and fatty acid synthetase activity increased 200% and 455%, respectively, between 1 and 5 days. dexamethasone (10 nm) stimulated fatty acid sy ... | 1990 | 2297514 |
| calcitonin gene-related peptide stimulates irregular spiking and transit of contents in the small intestine of the rat. | 1990 | 2327270 | |
| inhibitory effects of neuropeptide y on adenylate cyclase of rabbit ciliary processes. | the inhibitory effect of neuropeptide y (npy) was studied on the adenylate cyclase (ac) activity in homogenates of rabbit ciliary processes and compared with that of the alpha 2-adrenergic agonist clonidine (cln). npy inhibited basal ac activity as well as ac activity stimulated by isoproterenol (iso), vasoactive intestinal polypeptide (vip) or forskolin (fsk). the extent of this inhibition corresponded well to the inhibition elicited by cln. the inhibitory effects of npy and cln appeared to be ... | 1990 | 2335109 |
| requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins. | by analysis of a temperature-sensitive yeast mutant, a heat-shock protein in the matrix of mitochondria, mitochondrial hsp70 (ssc1p), is found to be involved both in translocation of nuclear-encoded precursor proteins across the mitochondrial membranes and in (re)folding of imported proteins in the matrix. | 1990 | 2234077 |
| kinetic study of esterification by immobilized lipase in n-hexane. | the kinetic of the esterification of oleic acid by ethanol catalyzed by immobilized lipase of mucor miehei in n-hexane as a solvent has been completely studied. the kinetics of the reaction are suggested to agree with a ping-pong bi bi mechanism in which only inhibition by excess of ethanol has been identified. values of all apparent kinetic parameters were computed. no evidence of any significant external diffusional limitation which could account for these values has been detected. optimizatio ... | 1990 | 2265698 |
| central neural pathways for angiotensin-induced thirst. | evidence is reviewed implicating the preoptic region in angiotensin-induced thirst. the most responsive area according to results obtained with behavioral, electrophysiological, and autoradiographic mapping techniques is at the caudal border of the medial preoptic region and rostral border of the anterior hypothalamus. the neural pathway from this preoptic site for angiotensin-induced thirst extends along the medial forebrain bundle through the midlateral hypothalamus to the paramedial midbrain ... | 1990 | 213317 |
| processing of the glycoprotein of feline immunodeficiency virus: effect of inhibitors of glycosylation. | the processing and transport of the envelope glycoprotein complex of feline immunodeficiency virus (fiv) in the persistently infected crandell feline kidney (crfk) cell line were investigated. pulse-chase analyses revealed that the glycoprotein is synthesized as a precursor with an mr of 145,000 (gp145) and is quickly trimmed to a molecule with an mr of 130,000 (gp130). treatment of gp130 with endoglycosidase h (endo h) resulted in a protein with an mr of 75,000, indicating that nearly half the ... | 1991 | 1847441 |
| cardiomyopathy and woolly haircoat syndrome of hereford cattle. | 1991 | 2043089 | |
| [leiomyosarcoma of the right ventricle. report of a case and review of the literature]. | primary cardiac leiomyosarcoma is a very rare condition. only 15 cases have been reported in the literature. we report the case of a 27 year old man admitted to hospital for chest pain in march 1985. echocardiography showed a right ventricular tumour which was completely resected at surgery. the diagnosis of leiomyosarcoma was confirmed by histological examination. after 22 months follow-up, the patient was still alive despite pulmonary metastases. the diagnosis of cardiac tumour used to be made ... | 1991 | 1759901 |
| glucose and glutamine metabolism in rat macrophages: enhanced glycolysis and unaltered glutaminolysis in spontaneously diabetic bb rats. | metabolism of glutamine (gln, 2 mm) and glucose (5 mm) was studied in vitro in isolated resident peritoneal macrophages from both normal (bbn) and spontaneously diabetic bb (bbd) rats. the major products from gln were ammonia, glutamate, co2 and to a lesser extent aspartate. glucose decreased (p less than 0.01) the production of ammonia, co2 and aspartate from gln by 34-60%, but had no effect on the amount of glutamate accumulated. the major products from glucose were lactate and to a much lesse ... | 1991 | 1764469 |
| effect of spectrin on structure properties of lipid bilayers formed from mixtures of phospholipids. fluorescence and microcalorimetric studies. | effect of spectrin from human erythrocytes on structure properties of lipid bilayers formed from a mixture of phosphatidylethanolamine/phosphatidylserine (pe/ps) and/or phosphatidylethanolamine/phosphatidylcholine (pe/pc) was studied with the use of fluorescence and microcalorimetric methods. spectrin did not affect the order parameter of lipids in pe/ps vesicles. however, spectrin binding to liposomes did influence temperature, half-width and enthalpy of phase transitions in mixtures of dimyris ... | 1991 | 1769515 |
| magnetic maps in pigeons. | the homing of racing pigeons is often slowed down on days of high sun-spot activity or variability in the earth's magnetic field. the orientation of ring-billed gull chicks as well as the vanishing bearings of homing pigeons also seem to change in response to variability in the earth's magnetic field. furthermore, homing pigeons released under sunny skies at locations where the earth's magnetic field is distributed are disoriented, yet pigeons equipped with devices that change the magnetic field ... | 1991 | 1838522 |
| a monoclonal antibody against the sulfidopeptide leukotrienes ltc4, ltd4 and lte4. | a monoclonal antibody (1a-ldr1) against sulfidopeptide leukotrienes (lt) is described. the mab shows a nearly identical detection limit of about 0.04 ng for ltc4, ltd4, lte4 and naclte4 in standard fluid phase ria. steric modifications, however, diminish the sensitivity, as determined for the examples 5-epi-ltc4, 6-epi-ltc4, 5,6-epi-ltc4 and 11-trans-ltc4. no crossreactivity could be observed for ltb4. crossreactions with components of the lt peptide chain such as l-cysteine or glutathione, as w ... | 1991 | 1847833 |
| effects of prolonged inhalation of n-formyl-methionyl-leucyl-phenylalanine in rabbits. | on the basis of its potent proinflammatory and spasmogenic effects, n-formyl-methionyl-leucyl-phenylalanine (fmlp), a bacterial oligopeptide, is a putative mediator of bronchoconstriction and airway inflammation during bacterial bronchial infection. however, after an fmlp dose-response curve in rabbits, tachyphylaxis to a second challenge was seen in some rabbits and airway inflammation was absent. this study was designed to reproduce the more prolonged airway exposure to fmlp that may occur dur ... | 1991 | 1885438 |
| modulation of mycotoxin and nitrosamine carcinogenesis by indole-3-carbinol: quantitative analysis of inhibition versus promotion. | the value of chemopreventive agents for reducing human response to mycotoxins and n-nitrosamines remains uncertain, especially since many such agents also can act as tumour promoters. indole-3-carbinol (i3c) from cruciferous vegetables can inhibit dna adduction and hepatocarcinogenesis induced by aflatoxin b1 (afb1) or n-nitroso-diethylamine in trout if given before and with the carcinogen but promotes carcinogenesis when given after initiation. similar results have been obtained with i3c and af ... | 1991 | 1906835 |
| hyperperfusion and enhanced metabolic state of the brain during sudden lowering of the cutaneous temperature in rats. | forty-eight anesthetized and artificially ventilated rats were pretreated with the ganglion blocking agent, pentolinium (5 mg/kg, i.v.), followed by continuous phenylephrine infusion for maintenance of normotension. the rats were subjected to mock treatment (16 rats), to sudden exposure to thermoneutral water (34-36 degrees c, 16 rats), or to cold water (16 degrees c, 16 rats). the latter treatment lowered the cutaneous temperature to 25-30 degrees c. neither one of these treatments produced any ... | 1991 | 1940032 |
| helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (macaca nemestrina) | a new microaerophilic, spirally curved, rod-shaped bacterium was isolated from the gastric mucosa of a pigtailed macaque (macaca nemestrina). the gram-negative cells of this bacterium are oxidase, catalase, and urease positive and strongly resemble helicobacter pylori (campylobacter pylori) cells. like h. pylori, this organism does not metabolize glucose, does not reduce nitrate or produce indole, does not produce h2s from triple sugar iron agar, does not hydrolyze hippurate or esculin, and does ... | 1991 | 1995031 |
| adrenalin activation of beta 2-adrenoceptors stimulates morphological changes in astrocytes (pituicytes) cultured from adult rat neurohypophyses. | neurohypophysial astrocytes, the pituicytes, are known to undergo morphological changes in vivo in response to stimuli that increase the demand for hormone secretion. similar changes have been induced by beta-adrenergic stimulation both in the isolated, but otherwise intact, neural lobe and in pituicytes cultured from adult rats. since the predominant beta-receptor subtype in the neural lobe is beta 2, we investigated the possibility that beta 2-receptor activation is mainly responsible for the ... | 1991 | 1682016 |
| [ddt sensitivity level of the mosquitoes in an anopheles maculipennis meig. complex in the moldavian ssr]. | 1991 | 160008 | |
| [nonexcreting waldenstrom's disease: a further case with anaplastic cancer of the lung and amyloidosis]. | 1991 | 208058 | |
| cytochrome c from schizosaccharomyces pombe. 2. amino-acid sequence. | the amino acid sequence of schizosaccharomyces pombe cytochrome c has been established by automatic degradation of the protein and by manual degradation of fragments obtained by cyanogen bromide cleavage and chymotryptic digestion. the chymotryptic peptides were aligned by homology with other known cytochrome c sequences. the protein is 108 residues long, with a four-residue amino-terminal tail. it has only one methionine residue and differs from other fungal cytochromes c in lacking the one-res ... | 1992 | 207525 |
| mouse hypersensitivity pneumonitis: depletion of nk cells abrogates the spontaneous regression phase and leads to massive fibrosis. | the contribution of natural killer cells (nk) in the progression of mouse hypersensitivity pneumonitis induced by repeated intranasal instillations with the thermophilic actinomycete faeni rectivirgula was examined. these instillations determined a very large increase in the lung index (ca. twofold at 3 weeks), used as a measure of inflammation. in addition, this instillation was associated with a tenfold increase in the number of cells recovered in the bronchoalveolar lavage (bal) at 3 weeks an ... | 1992 | 1468409 |
| use of an enzyme-linked immunosorbent assay to monitor the control of staphylococcus aureus mastitis. | conventional culture methods were used to evaluate the ability of an elisa to identify staphylococcus aureus imi. the test was 96% accurate; sensitivity was 90%, and specificity was 97%. the test was used to screen preserved milk samples rapidly in 10 cooperator herds. prevalence of imi was greater than 10% in 6 herds at the first test. average prevalence of cows scoring +2 (suspect) and +3 (positive) was 12.6%. prevalence declined during the 12-mo study. incidence of new imi decreased from 7.9% ... | 1992 | 1560137 |
| alpha 2-adrenoceptor density in forebrain areas of zucker rats: no effect of genetic obesity or antiglucocorticoid treatment. | the density of alpha 2-adrenoceptors was determined in different forebrain regions of lean and obese zucker rats by para-[125i]iodoclonidine ([125i]pic) receptor autoradiography. no significant differences were observed between the zucker phenotypes in the density of alpha 2-adrenoceptors in any brain areas examined. four days treatment with the antiglucocorticoid mifepristone (ru 38486) did not affect [125i]pic-binding. it is concluded that the hyperphagia and abnormal corticosterone secretion ... | 1992 | 1322226 |
| structural and functional analyses of the repressor, rbsr, of the ribose operon of escherichia coli. | the dna sequence encoding the rbs repressor protein, rbsr, has been determined. amino acid sequence analyses of the product of an rbsr-lacz fusion and of affinity-purified rbsr demonstrate that translation begins at an unusual codon, ttg, and that the initial amino acid is removed during maturation of the protein. dna-binding assays indicate that rbsr binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site and that the affinity for the rbs operator is reduce ... | 1992 | 1304369 |
| ab-interno neodymium:yag versus erbium:yag laser sclerostomies in a rabbit model. | this study was undertaken to determine whether thermally-induced tissue necrosis was a factor in ab-interno contact-laser sclerostomy failure. a rabbit model was used to compare the continuous-wave neodymium (nd):yag with the pulsed erbium (er):yag laser with respect to such failure. laser energy was focused into a fused-silica fiber optic (400 microns) for the nd:yag laser (12 w; 3 to 5 seconds), and into a single-crystal, uncladded sapphire fiber optic (250 microns) for the er:yag laser (7 to ... | 1992 | 1574289 |
| gastric uptake of gallium-67 in aids. | we describe gastric localization of 67ga in 13 patients with acquired immunodeficiency syndrome (aids) among 148 referred primarily to rule out pneumocystis carinii pneumonia (pcp). endoscopic biopsies in five of the patients indicated cytomegalovirus (cmv) infection in one, gastritis in two, and normal tissue in two. other associated, but nongastric, infections in these 13 patients included esophageal candidiasis, pcp, mycobacterium avium-intracellulare (mai) complex, coccidioidomycosis, toxopl ... | 1992 | 1314894 |
| leukotriene b4 omega-oxidation by human polymorphonuclear leukocytes is inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa. | human polymorphonuclear leukocytes (pmnl) metabolize the potent chemotaxin leukotriene b4 (ltb4) by omega-oxidation to 20-hydroxyl-ltb4 and 20-carboxy-ltb4. the ability of unstimulated human pmnl to metabolize exogenous ltb4 was found to be inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa, in a dose-dependent manner. 1-hydroxyphenazine (1-ohp), a metabolite of pyocyanin, was not inhibitory under identical conditions. the initial enzymic step in the conversion of ... | 1992 | 1316878 |
| characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis. | alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec 1.1.1.132) is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ... | 1992 | 1370473 |
| biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis. | the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ... | 1992 | 1370931 |
| antagonism of the motor effects of cgrp and of capsaicin on the guinea pig ileum by human cgrp8-37. | 1992 | 1637118 | |
| antibacterial peptides designed as analogs or hybrids of cecropins and melittin. | eight new analogs of cecropin a, two new analogs of melittin and 30 hybrid peptides containing sequences from cecropins and melittin have been synthesized. the lengths of the peptides have varied from 37 residues (the length of cecropin a) to 18 residues. the peptides have been assayed for lysis of sheep red blood cells and for antibacterial activity against two gram negative and three gram positive bacteria. the best analogs of cecropin a maintained the anti-escherichia coli activity of the par ... | 1992 | 1483838 |
| pluridirectional tomography in the evaluation of pituitary tumors. | a series of 110 patients with suspected pituitary tumors was recently evaluated by endocrinologic and neuroradiologic studies. of the 77 patients in this series who underwent surgery, 74 had confirmed pituitary adenomas, two had suprasella tumors of the influndibulum, and one had an empty sella not previously diagnosed at pneumoencephalography. all 17 patients with normal plain skull films but abnormal thin section tomography who underwent surgery had documented pituitary tumors. this includes s ... | 1993 | 413394 |
| [social work in public health service undergoing changes - new tasks and aims (author's transl)]. | 1993 | 158724 | |
| how do cardiac cells die? | 1993 | 45825 | |
| use of doppler ultrasound in intestinal surgery. | doppler ultrasonography was used intraoperatively in 117 patients undergoing intestinal anastomosis or enterostomy to determine the adequacy of blood supply at the margins of resection. doppler findings were compared with clinical assessment of intestinal blood flow by the operating surgeon. in 92 per cent of cases, doppler signals and clinical observation coincided. however, in five of six cases in which doppler signals were absent at one margin, the surgeon resected additional intestine, selec ... | 1994 | 159631 |
| [prostaglandins and bronchial asthma (author's transl)]. | prostaglandins (pg) show different effects on the bronchomotoric excitability depending on the fact to which group they certain. the best investigated pg are pge1, pge2 (bronchodilators) and pgf2a (bronchoconstrictor). it is possible that pg play a certain role in the pathogenesis of bronchial asthma. among other hypotheses, the influence upon adenylate cyclase - camp systems (without affecting the adrenoreceptors) is evident. probably, pg exert a regulatory function on bronchial tone. the patho ... | 1994 | 205127 |
| [french society of perinatal medicine (la baule, november 1978)]. | 1994 | 44549 | |
| [hygienic evaluation of the operating efficiency of a radial-type air oxidizer for sewage purification]. | 1994 | 159220 | |
| investigation on the kinetic mechanism of octopine dehydrogenase. a regulatory behavior. | the kinetic scheme of octopine dehydrogenase of pecten maximus l., a monomeric enzyme obeying a bi-ter sequential mechanism, was completed, essentially in the forward reaction, by steady-state studies over a wide range of substrate concentration at ph 7.0. deviation from the michaelis-menten behavior with respect to nad+ and other significant kinetic data led us to ascribe for octopine dehydrogenase mechanism the mnemonical enzyme concept. in addition, another regulatory behavior can be envisage ... | 1994 | 205413 |
| essentials of noise regulations. | 1995 | 157458 | |
| [early diagnostic studies in babyhood and infancy]. | 1995 | 161391 | |
| accident compensation: gates and gatekeepers. | 1996 | 2143261 | |
| the evolution of quality of life evaluations in cancer therapy. | patients' feelings, psychological and functional status, and quality of life have often been regarded as unmeasurable subjective entities that cannot be scientifically studied. but now, with better measurement tools, the cancer patient's quality of life can be understood in more operational terms than the "soft" subjective statements that have too often characterized the field to date. this paper addresses the issue of quality of life from several perspectives: 1. historical antecedents of the c ... | 1996 | 2143407 |
| [selection of rational methods of preanesthetic medication in surgical treatment of glaucoma]. | 1996 | 38419 | |
| [the value of the excretion urogram following cerebral angiography (author's transl)]. | routine abdominal films were obtained after 100 carotid angiograms in order to see the excretion urogram. in 64 patients the contrast was adequate for diagnosis. in twelve of these a significant finding was observed. this simple additional examination was particularly valuable in searching for a primary tumour when the angiogram had demonstrated cerebral metastases. | 1996 | 157929 |
| [lumbar spinal osteochondrosis in children]. | 1996 | 161014 | |
| [bronchial carcinoma with secretion of acth and hcg (author's transl)]. | 1996 | 178162 | |
| histochemical, ultrastructural and hormonal studies on the pars distalis of the echidna (tachyglossus aculeatus). | there were no consistent significant differences between the concentrations of luteinizing hormone (lh) and adrenocorticotrophin (acth) in the rostral compared with the caudal zone of the echidna pars distalis. this suggests that lh is secreted by cells containing s-type granules (probably corresponding to secretory vesicles 200-300 nm diameter) which are distributed throughout the gland. some of the cells containing vesicles 100-200 nm diameter, seen in small numbers in both zones of the gland, ... | 1996 | 167975 |
| total exchangeable and red cell potassium in patients with chronic renal failure. | 1996 | 1217999 | |
| [use of nonachlazine in the treatment of chronic ischemic heart disease]. | 1996 | 452467 | |
| some personal thoughts on the treatment of children with anaesthesia. | 1997 | 156964 | |
| whither breast cancer? report on the inaugural meeting of the national breast cancer group. | 1997 | 23587 | |
| caudal dislocation of the pons in the adult arnold-chiari malformation: an angiographic evaluation. | the position of the pons, determined angiographically, can be related to the clivus and sella. in adult patients with the chiari malformation, a caudal dislocation of the pons is frequently associated with the well known anomalously low position of the cerebellar tonsils. this finding may aid in the angiographic diagnosis of the chiari malformation. | 1997 | 179349 |
| structure of the mengo virion. iv. amino- and carboxyl-terminal analyses of the major capsid polypeptides. | 1997 | 179198 | |
| recurrent intussusception due to a primary colloid carcinoma of the large bowel in a child. | 1997 | 179075 | |
| orofacial dyskinesia. clinical features, mechanisms and drug therapy. | orofacial or tardive dyskinesias are involuntary repetitive movements of the mouth and face. in most cases, they occur in older psychotic patients who are in institutions and in whom long-term treatment with antipsychotic drugs of the phenothiazine and butyrophenone groups is being carried out. these dyskinesias are frequent in occurrence and characteristically are irreversible. several biochemical mechanisms have been proposed as causes, including hypersensitivity or partially deneverated brain ... | 1997 | 23611 |
| [epidemiology of lumbar pain in patients at a general polyclinic in santiago, chile]. | 1997 | 3155951 | |
| proceedings: an overview of clinical applications of photon absorptiometry. | 1997 | 179420 | |
| effect of early thymectomy on the developing chick embryo [proceedings]. | 1998 | 317044 | |
| the effect of bicarbonate and distilled water on sickle cell trait hematuria and in vitro studies on the interaction of osmolality and ph on erythrocyte sickling in sickle cell trait. | the effect of intravenously administered distilled water was examined alone and during alkalization in a patient with gross hematuria associated with the sickle cell trait. on each of 4 occasions hematuria ceased promptly after the infusion of distilled water. bicarbonate therapy also consistently decreased hematuria. in vitro studies on erythrocytes from another patient with sickle cell trait and hematuria demonstrated that slight increases in urinary ph similar to those that occur in the urine ... | 1998 | 21309 |