| studies on the mechanism of oxidative phosphorylation. positive cooperativity in atp synthesis. | kinetic and nucleotide binding studies have shown that submitochondrial particles from bovine heart possess three exchangeable binding sites for adp or gdp. in order of decreasing affinity at neutral ph, these sites will be referred to as sites i, ii, and iii, and their respective dissociation constants as ki, kii, and kiii. in oxidative phosphorylation experiments in the presence of saturating amounts of inorganic phosphate, rapid atp (or gtp) synthesis occurred only upon adp (or gdp) binding t ... | 1990 | 2294123 |
| glucocorticoid stimulation of fatty acid synthesis in explants of human fetal lung. | we examined the effects of glucocorticoids and thyroid hormone (t3) on fatty acid synthesis, fatty acid composition and fatty acid synthetase activity in explants of human fetal lung (16-23 wk gestation). explants were cultured 1-7 days in the absence (control) or presence of dexamethasone (10 nm) and/or t3 (2 nm). in control explants fatty acid synthesis and fatty acid synthetase activity increased 200% and 455%, respectively, between 1 and 5 days. dexamethasone (10 nm) stimulated fatty acid sy ... | 1990 | 2297514 |
| calcitonin gene-related peptide stimulates irregular spiking and transit of contents in the small intestine of the rat. | | 1990 | 2327270 |
| inhibitory effects of neuropeptide y on adenylate cyclase of rabbit ciliary processes. | the inhibitory effect of neuropeptide y (npy) was studied on the adenylate cyclase (ac) activity in homogenates of rabbit ciliary processes and compared with that of the alpha 2-adrenergic agonist clonidine (cln). npy inhibited basal ac activity as well as ac activity stimulated by isoproterenol (iso), vasoactive intestinal polypeptide (vip) or forskolin (fsk). the extent of this inhibition corresponded well to the inhibition elicited by cln. the inhibitory effects of npy and cln appeared to be ... | 1990 | 2335109 |
| treatment of behavioral disorders in animals. | | 1990 | 2386258 |
| [immunohistochemical study and lectin distribution of thyroid carcinoma originated from follicular epithelium]. | 97 cases of thyroid carcinoma originated from follicular epithelium were investigated by using histological and immunohistochemical techniques with special reference to lectin distribution. according to the who histological typing of thyroid tumours, these cases were divided into three categories as follows: papillary carcinoma of thyroid (pct) 56, follicular carcinoma of thyroid (fct) 31 and undifferentiated carcinoma of thyroid (uct) 10. results showed that three different kinds of thyroid car ... | 1990 | 2390796 |
| simplified differential staining of mouse sister chromatids. | many previously developed methods for sister chromatid exchange (sce) analysis both in vivo and in vitro have been published during the past decade. such methods have met with success in many but not all laboratories. these methods often involve expensive, hazardous equipment such as ultraviolet light and mercury lamps, as well as tedious treatments with hot salt solutions, acids or enzymes. the authors have developed an in vivo method using a 50 mg bromodeoxyuridine (brdu) pellet combined with ... | 1986 | 2427279 |
| a 50-kda fragment from the nh2-terminus of the heavy subunit of clostridium botulinum type a neurotoxin forms channels in lipid vesicles. | 1. a 50-kda fragment representing the nh2-terminus of the heavy subunit of botulinum type a neurotoxin was found, at low ph, to evoke the release of k+ from lipid vesicles loaded with potassium phosphate. similar k+ release was also observed with the intact neurotoxin, its heavy chain and a fragment consisting of the light subunit linked the 50-kda nh2-terminal heavy chain fragment. the light subunit alone, however, was inactive. 2. in addition to k+, the channels formed in lipid bilayers by bot ... | 1987 | 2441987 |
| effects of irradiation on diabetes in the bb/wor rat. | lymphoid irradiation is known to prevent spontaneous autoimmune diabetes in susceptible bb rats. the present studies investigated further the effects of radiation in diabetes prone (dp) and resistant (dr) bb/wor rats, and histocompatible yoshida (yos) rats. single doses of total body gamma irradiation (125-600 rads) induced diabetes within 22-44 days in 20 of 102 (20%) 30 day old dr rats, less than 1% of which develop the disease. radiation was also associated with (1) a reduction in the ratio o ... | 1989 | 2491639 |
| [experimental evolutive cycle of diphyllobothrium erinaceieuropei in paracyclops fimbriatus, tadpoles of bufo arenarum and dogs]. | experiments were performed in order to develop the life cycle of diphyllobothrium erinaceieuropei rudolphi 1819 (cestoda, pseudophyllidea) in paracyclops fimbriatus and bufo arenarum as intermediate hosts and dogs as definitive hosts. the eggs of diphyllobothrium erinaceieuropei from faeces of naturally infected dogs were kept refrigered, in water. in order to obtain coracidiums they were incubated at 25 degrees c, and then were placed in a flask which contained paracyclops fimbriatus. the copep ... | 1989 | 2516918 |
| use of synthetic ribosome binding site for overproduction of the 5b protein of insertion sequence is5. | insertion sequence is5 is a bacterial transposable element which contains three open reading frames designated 5a, 5b and 5c. although there was no detectable expression from the 5b open reading frame when it was preceded by the native promoter and ribosome binding site or by a tac promoter and the native ribosome binding site, we have overproduced a 5b protein both in vitro and in escherichia coli cells by using a tac promoter and a specially-designed synthetic ribosome binding site. beta-galac ... | 1989 | 2538799 |
| effect of prolonged infusion of anf in normotensive and hypertensive monkeys. | it is now recognized that bolus and short-term infusions of atrial natriuretic factor (anf) into different species lead to a slight and transient decrease of blood pressure, while prolonged infusions cause a significant blood pressure reduction in hypertensive but not in normotensive rats. the present study was designed to evaluate the effects of prolonged anf infusions on blood pressure and humoral parameters in normotensive and hypertensive african green monkeys (cercopithecus aethiops). human ... | 1989 | 2553071 |
| the nature and function of polypeptide growth factor receptors in the human placenta. | | 1989 | 2561287 |
| proton nmr and cd solution conformation determination and opioid receptor binding studies of a dynorphin a(1-17) model peptide. | the opioid peptide dynorphin a(1-17) contains a peptide segment in residues 7-15 with the potential to form an amphiphilic beta-strand. this amphiphilic structure may, like the amphiphilic alpha-helices found in many other peptide hormones, be an important determinant of its interactions with membranes and receptors. in order to investigate and characterize these interactions, we have synthesized a 17-residue dynorphin analogue (yggflkkvkpkvkvkss) that incorporates a peptide model of this amphip ... | 1989 | 2574597 |
| effects of gestational exposure to phencyclidine: distribution and neurochemical alterations in maternal and fetal brain. | phencyclidine is a widely used drug of abuse and is known to produce a wide variety of psychoactive effects. pcp abuse by pregnant women has been reported to result in the birth of infants exhibiting irritability, jitteriness and hyperactivity with high pitched cries. the present study was designed to evaluate the distribution of pcp in the maternal and fetal brain and the neurochemical effects produced by gestational exposure. pregnant sprague-dawley rats were treated sc with 5 mg/kg pcp on 3 c ... | 1989 | 2576301 |
| cough associated with canine vaccination? | | 1989 | 2588459 |
| the surface electrocardiogram as a means of refining a murine pressure overload model of congestive heart failure. | | 1989 | 2590929 |
| the surface electrocardiogram as a means of refining a murine pressure overload model of congestive heart failure. | | 1989 | 2590929 |
| comparison of a 550,000 dalton cartilage matrix glycoprotein in cartilage from immature and mature dogs. | cartilage matrix glycoprotein is a disulfide bonded 550,000 dalton protein found in cartilage and in the vitreous of the eye. immunofluorescence studies using a specific antiserum to this glycoprotein have previously demonstrated a difference between the topographic distribution of staining for cartilage matrix glycoprotein in cartilage from immature and mature animals. using a polyclonal antiserum to cartilage matrix glycoprotein, we studied this protein in cartilage from normal immature and ma ... | 1989 | 2666656 |
| isolation of a high frequency donor of rhodobacter capsulatus by integration of the plasmid pmt1000 into the chromosome. | the temperature-independent clone of rhodobacter capsulatus ua7041, carrying the temperature-sensitive plasmid pmt1000, has been obtained by selection for plasmid markers at the non-permissive temperature. the transfer to escherichia coli of all drug resistance encoded by pmt1000 was of about 10(-7) when the donor was the ua7041 strain, and of 5 x 10(-4) when the donor was the temperature-sensitive strain of r. capsulatus. electrophoretic analyses showed no plasmid band typical of the autonomous ... | 1989 | 2679770 |
| safrole, eugenol and methyleugenol induce intrachromosomal recombination in yeast. | deletion of an integrated plasmid, a specific type of intrachromosomal recombination, was evaluated for inducibility with the phenylpropenes safrole, eugenol and methyleugenol in the yeast saccharomyces cerevisiae. these phenylpropenes are found in food products, spices, pharmaceuticals and clove cigarettes. safrole and eugenol are known carcinogens in animals and methyleugenol is a suspected carcinogen. these phenylpropenes are not detectable by the ames assay and most other short-term tests us ... | 1989 | 2685589 |
| safrole, eugenol and methyleugenol induce intrachromosomal recombination in yeast. | deletion of an integrated plasmid, a specific type of intrachromosomal recombination, was evaluated for inducibility with the phenylpropenes safrole, eugenol and methyleugenol in the yeast saccharomyces cerevisiae. these phenylpropenes are found in food products, spices, pharmaceuticals and clove cigarettes. safrole and eugenol are known carcinogens in animals and methyleugenol is a suspected carcinogen. these phenylpropenes are not detectable by the ames assay and most other short-term tests us ... | 1989 | 2685589 |
| the paramyxoviridae: aspects of molecular structure, pathogenesis, and immunity. | | 1989 | 2690597 |
| demonstration of axonal branching of fibres from certain precerebellar nuclei to the cerebellar cortex and nuclei: a retrograde fluorescent double-labelling study in the cat. | the projections from certain brain stem precerebellar nuclei to the cerebellar cortex and nuclei have been examined in the cat by using the retrograde fluorescent double-labelling technique. crystalline fluoro-gold was implanted into the left cerebellar nuclei from the contralateral side and rhodamine-b-isothiocyanate was injected into the overlying cerebellar cortex. the inferior olive, the lateral reticular nucleus, and the reticular tegmental pontine nucleus all contained double- as well as s ... | 1989 | 2707349 |
| demonstration of axonal branching of fibres from certain precerebellar nuclei to the cerebellar cortex and nuclei: a retrograde fluorescent double-labelling study in the cat. | the projections from certain brain stem precerebellar nuclei to the cerebellar cortex and nuclei have been examined in the cat by using the retrograde fluorescent double-labelling technique. crystalline fluoro-gold was implanted into the left cerebellar nuclei from the contralateral side and rhodamine-b-isothiocyanate was injected into the overlying cerebellar cortex. the inferior olive, the lateral reticular nucleus, and the reticular tegmental pontine nucleus all contained double- as well as s ... | 1989 | 2707349 |
| almitrine bismesylate reduces hypoxic pulmonary vasoconstriction in isolated rat lungs. | the purpose of this study is to test how almitrine bismesylate (alm) affects the function of pulmonary vasculature during normoxic ventilation, and whether low doses of alm not causing detectable vasoconstriction during normoxic ventilation potentiate hypoxic pulmonary vasoconstriction (hpvc). isolated wistar male rat lungs were perfused with homologous blood at constant flow, and venous and ventilatory pressure. in the first experiment, after equilibration, dose-response curves to alm (from 0 t ... | 1989 | 2711381 |
| carrageenan-induced pulmonary emphysema of rabbit. | a transbronchial injection of 0.75% carrageenan in physiologic saline induced pneumonia followed by emphysema in the insulted lobe. in the stages of pneumonia, scattered infiltration of polymorphonuclear leukocytes was seen throughout the affected lobe within a few days of treatment; later this was replaced by the accumulation of carrageenan-laden macrophages, which lasted for one to two months. enlargement of alveoli and alveolar ducts appeared 2 weeks to 2 months after the treatment, and pulmo ... | 1989 | 2711383 |
| pulsed er:yag- and 308 nm uv-excimer laser: an in vitro and in vivo study of skin-ablative effects. | using a pulsed xecl excimer laser (308 nm) and a pulsed er:yag laser (2,940 nm), we investigated skin ablation as a function of pulse number, radiant energy, and repetition rate. in vitro analysis of lesions performed in freshly excised human skin were consistent with in vivo results obtained from experiments on pig skin. pulsed 308 nm laser radiation caused considerable nonspecific thermal tissue injury followed by an inflammatory reaction and impaired healing of lesions in vivo. these findings ... | 1989 | 2716457 |
| the quantitation by radioimmunoassay of 2'-deoxyuridine 5'-triphosphate in extracts of thymidylate synthase-inhibited cells. | a radioimmunoassay (ria) for dutp, with a sensitivity of 3.78 fmol, has been developed. the antibody cross-reacted with dttp so that affinity purification of the immunoglobulin g fraction was required before its use in the ria. cross-reactivity with utp and with mono- and diphosphodeoxyuridylates has necessitated respectively sodium periodate oxidation and anion exchange chromatography of cell extracts, prior to ria quantitation of dutp directly in fractions from the chromatography column. mean ... | 1989 | 2729554 |
| type iii machado-joseph disease in a japanese family: a clinicopathological study with special reference to the peripheral nervous system. | the clinical and pathological findings in a 58-year-old japanese man suffering from type iii machado-joseph disease are reported. the patient became affected at the age of 50 years and presented cerebellar ataxia, progressive external ophthalmoplegia and muscular atrophy, although extrapyramidal signs were never detected throughout the whole course of his disease. his mother, sister and son presented progressive ataxia in the third or fourth decade. the mode of inheritance is considered to be au ... | 1989 | 2743650 |
| a fluorescence study of substrate and inhibitor binding to bovine liver dihydrofolate reductase. | 1. covalent coupling of fluorescein to methotrexate (mtx) by a 5-carbon spacer yields a dihydrofolate reductase (dhfr) inhibitor (fmtx) with ki = 11 nm. 2. fmtx shows a fluorescence quenching with respect to fluorescein which is relieved by binding to the enzyme. 3. the dissociation constants (kd) of mtx, fmtx, nadph and 7,8-dihydrofolate (dhf) from bovine liver dhfr have been determined by fluorometric titrations. 4. the kd values for nadph, mtx and fmtx from the complementary binary complexes ... | 1989 | 2744204 |
| time-resolved extrinsic fluorescence of aromatic-l-amino-acid decarboxylase. | the coenzyme-linked fluorescence of aromatic-l-amino-acid decarboxylase decays non-exponentially. the decay of both native and nabh4 reduced samples can only be fitted by two exponentials each roughly accounting for about half of the total fluorescence. denaturation of the reduced protein with 8 m urea makes the fluorescence decay mono-exponential, like that observed for the reference compound pyridoxamine-5-phosphate. an extra pyridoxyl moiety can be bound to the enzyme after incubation with ex ... | 1989 | 2752045 |
| [prospects for measuring the rheologic characteristics of human soft tissues based on the recording of their transverse rigidity]. | it is shown by an example of a forearm that to determine objective characteristics of rheological properties of soft human tissues by measuring their transverse stiffness (k) one should first investigate the relation between k and the diameter of the used indentor (d). the nonlinearity of the dependence k(d) for indentor diameters from 2 to 8 mm is demonstrated and it is assumed that this nonlinearity is due to heterogeneity of tissue properties in the directions normal to the axis of force acti ... | 1989 | 2765581 |
| decreased 3 alpha-androstanediol glucuronide levels in plasma and random urines in male pseudohermaphroditism caused by 5 alpha-reductase deficiency. | concentrations of 3 alpha-diol glucuronide (3 alpha-diol g) in plasma and/or random urine samples were determined in seven subjects with familial male pseudohermaphroditism (fmp) due to 5 alpha-reductase deficiency (5 alpha-rd). all subjects were natives of an isolated turkish village with a high incidence of consanguineous marriage. a specific and sensitive antibody to 3 alpha-diol was used for radioimmunoassay of 3 alpha-diol g after hydrolysis and chromatographic purification. the mean plasma ... | 1989 | 2770532 |
| selective infection of human cd4+ cells by simian immunodeficiency virus: productive infection associated with envelope glycoprotein-induced fusion. | simian immunodeficiency virus (siv) and human immunodeficiency virus share the property of tropism for cd4-bearing cells. infection is initiated by a high-affinity interaction between cd4 and conserved domains on the viral envelope glycoprotein. in this report, we demonstrated that siv had a restricted host range among human cd4+ cells when compared with human immunodeficiency virus type 1 or type 2. this restricted tropism was associated with the inability of the siv envelope glycoprotein to in ... | 1989 | 2784571 |
| effect of cck antagonists cr 1409 and cr 1505 on rat pancreatic exocrine secretion in vivo. | the action of cholecystokinin (cck) antagonists cr 1409 and cr 1505 on pancreatic exocrine secretion stimulated by exogenous and endogenous cck was studied in vivo in anesthetized rats, and compared with proglumide. intravenous administration of cr 1409 and cr 1505 in graded doses between 0.04 and 25 mg/kg/h resulted in a dose-dependent inhibition in pancreatic juice volume and amylase output stimulated by intravenous infusion of cck-8 in a dose of 0.06 microgram/kg/h. cr 1409 is 1,000 times and ... | 1989 | 2813333 |
| effect of cck antagonists cr 1409 and cr 1505 on rat pancreatic exocrine secretion in vivo. | the action of cholecystokinin (cck) antagonists cr 1409 and cr 1505 on pancreatic exocrine secretion stimulated by exogenous and endogenous cck was studied in vivo in anesthetized rats, and compared with proglumide. intravenous administration of cr 1409 and cr 1505 in graded doses between 0.04 and 25 mg/kg/h resulted in a dose-dependent inhibition in pancreatic juice volume and amylase output stimulated by intravenous infusion of cck-8 in a dose of 0.06 microgram/kg/h. cr 1409 is 1,000 times and ... | 1989 | 2813333 |
| a clinical trial using a regimen which includes a norgestomet implant and norgestomet plus oestradiol valerate injection as a treatment for anoestrus in dairy cows. | in seasonally calving dairy herds in the macalister irrigation area of gippsland, victoria, cows that had not been observed in oestrus by the start of the mating season and which had inactive ovaries based on rectal palpation and progesterone assay were treated with a hormonal treatment (n = 49). mature cows had calved at least 40 days previously and 2-year-olds had been calved at least 60 days. the treatment consisted of a norgestomet implant and a norgestomet and oestradiol injection on day 1; ... | 1987 | 2820369 |
| the effects of dietary fish oil on hepatic high density and low density lipoprotein receptor activities in the rat. | rats were fed either a standard ration diet or that diet supplemented with 8% by wt of a marine fish oil or safflower oil. after 10 days, plasma triacylglycerols, total cholesterol, high density lipoprotein (hdl) cholesterol, hepatic cholesterol and fatty acid synthesis and hepatic low density lipoprotein (ldl) receptor activity were significantly depressed while hdl receptor activity was significantly increased in rats fed fish oil. fish oil-induced effects on cholesterol metabolism in the rat ... | 1987 | 2820793 |
| the effects of dietary fish oil on hepatic high density and low density lipoprotein receptor activities in the rat. | rats were fed either a standard ration diet or that diet supplemented with 8% by wt of a marine fish oil or safflower oil. after 10 days, plasma triacylglycerols, total cholesterol, high density lipoprotein (hdl) cholesterol, hepatic cholesterol and fatty acid synthesis and hepatic low density lipoprotein (ldl) receptor activity were significantly depressed while hdl receptor activity was significantly increased in rats fed fish oil. fish oil-induced effects on cholesterol metabolism in the rat ... | 1987 | 2820793 |
| onset of steroidogenesis and differentiation of functional lh receptors in rat fetal testicular cultures. | the present study was performed to investigate in vitro the onset of steroidogenesis and the responsiveness to lh in rat fetal testes. the male gonads explanted on days 12.5, 13.5, and 14.5 of gestation in m199 produced testosterone from 15.5 days as is the case in vivo dcamp (1 mm) induced an anticipated steroidogenesis on day 14.5 with secretions of testosterone (0.026 +/- 0.003 ng/gonad/24 h) and progesterone (0.078 +/- 0.005 ng/gonad/24 h), whereas lh (100 ng/ml) has no effect. antiandrogens ... | 1987 | 2823733 |
| insertion sequence is2 in the cyanobacterium chlorogloeopsis fritschii. | a cloned dna fragment, previously demonstrated to encode ribulose bisphosphate carboxylase/oxygenase (rubisco) of chlorogloeopsis fritschii strain ccap1411/1b, is shown also to include the entire transposable element, is2, normally a resident in the escherichia coli genome. southern-blot hybridisation experiments confirm the presence of is2 in the c. fritschii genome. this finding adds a new and unrelated species to the known host range of this element and provides evidence of genetic transfer b ... | 1988 | 2844631 |
| serum and vitreous herpes simplex antibodies in noninfected eyes. | recently, vitreous herpes simplex virus [hsv] antibody titers have been reported from patients with presumed herpetic retinitis. to help understand the significance of these data, we evaluated the hsv type 1 vitreous and serum antibodies in eight patients undergoing pars plana vitrectomy for diabetic vitreous hemorrhage, diabetic traction detachment, or rhegmatogenous retinal detachment. undiluted vitreous and diluted serum specimens were tested for hsv igg antibodies using the fluorescence immu ... | 1988 | 2847623 |
| the potassium channel opening action of pinacidil; studies using biochemical, ion flux and microelectrode techniques. | in rat aorta and rat portal vein, (-)- and (+)-pinacidil each produced a concentration-dependent inhibition of tension development. although the (-) isomer was the more potent, concentration effect curves for each isomer were steep with similar slopes. in rat portal vein, tetraethylammonium and procaine antagonised the relaxant effect of (+/-)-pinacidil, whereas 3,4-diamino-pyridine was without effect. intracellular microelectrode recording in rat portal vein showed that low concentrations of (+ ... | 1988 | 2848206 |
| the role of diffusible molecules in regulating the cellular differentiation of dictyostelium discoideum. | a central problem in developmental biology is to understand how morphogenetic fields are created and how they act to direct regionalized cellular differentiation. this goal is being pursued in organisms as diverse as moulds, worms, flies, frogs and mice. each organism has evolved its own solution to the challenge of multicellularity but there appear to be common underlying principles and, once pattern formation is fully understood in any system, some general truths seem certain to be revealed. a ... | 1988 | 2848678 |
| avian (chicken) parathyroid hormone: synthesis and comparative biological evaluation of the 1-34 fragment. | the full-length amino acid sequence of the avian (chicken) form of parathyroid hormone (cpth) has recently been elucidated. we have chemically synthesized, purified to a high degree, and analytically and biologically characterized the n-terminal 1-34 fragment of the avian hormone. the biological properties of cpth-(1-34)nh2 were evaluated and compared to the bovine fragment bpth-(1-34) in several assays. the potency of cpth-(1-34)nh2 in binding to pth receptors, in stimulating adenylate cyclase ... | 1988 | 2848691 |
| a library of monoclonal antibodies for exploring unknown functional molecules on the surface of synaptic membrane. | | 1988 | 2853561 |
| effect of the growth of anaerobic bacteria on the surface ph of solid media. | changes in surface ph occurring after varying periods of anaerobic incubation were measured for a total of 23 test solid media. there was little change in the surface ph of uninoculated plates, but plates inoculated with bacteriodes fragilis showed a striking fall in ph, to ph 5 in the case of some of the test media. the problems of controlling the surface ph of solid media are discussed and possible methods of control are considered. | 1985 | 2860132 |
| chloramphenicol in developing countries. | | 1985 | 2865547 |
| zinc absorption in inflammatory bowel disease. | zinc absorption was measured in 29 patients with inflammatory bowel disease and a wide spectrum of disease activity to determine its relationship to disease activity, general nutritional state, and zinc status. patients with severe disease requiring either supplementary oral or parenteral nutrition were excluded. the mean 65zncl2 absorption, in the patients, determined using a 65zn and 51cr stool-counting test, 45 +/- 17% (sd), was significantly lower than the values, 54 +/- 16%, in 30 healthy c ... | 1986 | 2873002 |
| enzymes of catecholamine metabolism in the brains of rat strains differing in their preference for or tolerance of ethanol. | the activities of the catecholamine-synthesizing and inactivating enzymes were determined in whole brains of two pairs of rat strains differing in their genetically-determined behavioural responses to ethanol. the alcohol-tolerant (at) rats did not show any significant differences in enzyme activities when compared with the non-tolerant (ant) strain. the activity of tyrosine hydroxylase was found to be significantly higher in brains of the alcohol-preferring (aa) rats, than in those of the alcoh ... | 1986 | 2874817 |
| spontaneous oscillation of the systemic arterial pressure during cardiopulmonary bypass in man. the effects of some drugs used during the operation. | despite the fact that the blood flow maintained during cardiopulmonary bypass is rather steady in the extracorporeal pump output, the arterial pressure under high-dose fentanyl anaesthesia quite often shows sinusoidal oscillations. in the present study the duration of an oscillatory cycle was on average 17.6 +/- 3.6 s, its amplitude 7.3 +/- 1.6 mm hg and mean systemic arterial pressure 75 +/- 12 mm hg. the oscillation is affected by the drugs used under bypass conditions. in our series of clinic ... | 1987 | 2886117 |
| human gastrin (gas) rflp recognised by digestion with bamhi or ecori. | | 1987 | 2889191 |
| somatostatin receptors on pituitary somatotrophs, thyrotrophs, and lactotrophs: pharmacological evidence for loose coupling to adenylate cyclase. | pharmacological characterization of somatostatin (srif) receptors located on somatotrophs, thyrotrophs, and lactotrophs was attempted by measuring the effects of 14 structural agonists of somatostatin (srif) on the inhibition of basal and grf-stimulated gh and basal and trh-stimulated prl and tsh secretion. we also checked the abilities of the analogs to displace [125i]n-tyr-srif binding to pituitary cell membranes and their potency to inhibit adenylate cyclase activity. there was a very good co ... | 1987 | 2890515 |
| transitory cardiovascular responses to rapid infusion of blood into aorta of rabbit. | blood (5 to 15 ml) was infused into aorta of the anesthetized rabbit through the carotid artery or femoral artery at a constant rate (0.55 to 8.3 ml/s). the carotid sinuses were occluded. the systemic arterial pressure (sap) began to rise immediately at the onset of the infusion and dropped gradually in most cases during the infusion. sap continued to decline after the infusion and in many cases became lower than the preinfusion pressure. within seconds, sap tapered off to its lowest point and r ... | 1988 | 2902240 |
| impaired oxygenation of gastric mucosa in portal hypertension. the basis for increased susceptibility to injury. | increased susceptibility to mucosal damage is a prominent feature of portal hypertensive gastropathy. since the portal hypertensive gastric mucosa has extensive microvascular changes, we postulated that the increased sensitivity to mucosal damage could have an ischemic basis. we measured distribution of gastric serosal and mucosal oxygenation in a group of portal hypertensive and sham-operated rats, and then studied the effects of intragastric aspirin. in the basal state, gastric mucosa of porta ... | 1989 | 2914542 |
| lithium and the neonate: developmental and metabolic aspects. | the interrelationship between lithium salts, which has been used in clinical trials of alcoholism, and ethanol, which evoke fetal alcohol syndrome by chronic use, was studied. this was made by evaluating the effect of prenatal and postnatal exposure to licl on ethanol and acetaldehyde metabolizing enzymes in the newborn mouse. the three li-treatment regimens made consisted of acute and short-term prenatal exposure to licl in addition to postnatal exposure until weaning of the neonates. the weane ... | 1986 | 2941034 |
| serine incorporation into the selenocysteine moiety of glutathione peroxidase. | the selenium in mammalian glutathione peroxidase is present as a selenocysteine ([se]cys) moiety incorporated into the peptide backbone 41-47 residues from the n-terminal end. to study the origin of the skeleton of the [se]cys moiety, we perfused isolated rat liver with 14c- or 3h-labeled amino acids for 4 h, purified the gsh peroxidase, derivatized the [se]cys in gsh peroxidase to carboxymethylselenocysteine ([se]cys(cm)), and determined the amino acid specific activity. perfusion with [14c]cys ... | 1987 | 2948953 |
| [clinical characteristics the ent complications of patients with type a (h3n2) influenza during the influenza epidemic in leningrad]. | | 1985 | 2951903 |
| structural and metabolic correlates of cell injury in the hypertrophied myocardium during valve replacement. | to characterize the ultrastructural and metabolic changes occurring in the hypertrophied ventricle during cardiac operations in man, we studied 36 patients with valvular heart disease undergoing valve replacement, during which multiple doses of cold potassium cardioplegic solution were administered (group i). each patient had substantial ventricular hypertrophy according to measurements made of left ventricular mass, with a mean of 232.1 +/- 19.8 gm/m2 (normal: 92 +/- 16 gm/m2). serial biopsy sp ... | 1987 | 2952840 |
| transcriptional mapping and nucleotide sequence of the escherichia coli fepa-fes enterobactin region. identification of a unique iron-regulated bidirectional promoter. | the iron-controlled fepa and fes-entf transcripts from the escherichia coli enterobactin gene complex are expressed divergently from a limited genetic region, thereby suggesting the existence of a single, possibly overlapping promoter junction for these genes. the nucleotide sequence of a 1,997-base pair hpai fragment specific for this genetic region allowed for the identification of an 1,122-base pair open reading frame as the previously uncharacterized fes gene. its product, fes (approximately ... | 1988 | 2974033 |
| improved localization of intracellular sites of phosphatases using cerium and cell permeabilization. | a simple permeabilization method has been developed that allows for intracellular localization of acid phosphatase in neutrophils and several types of tissue culture cells with cerium. this permeabilization procedure also facilitates intracellular alkaline phosphatase localization in neutrophils without the loss of cell surface reaction in this cell type. only the cell surface reaction was detected in the absence of permeabilization. glutaraldehyde-fixed cells were permeabilized with detergent d ... | 1985 | 2991362 |
| effects of tunicamycin on ngf binding and neurite outgrowth in pc12 cells. | the effects of inhibition of glycosylation on nerve growth factor (ngf) binding and neurite outgrowth response of pc12 cells have been examined. exposure to tunicamycin (1-10 micrograms/ml) for 24-36 hr eliminates the rapidly dissociating component of ngf binding and decreases the proportion of pc12 cells capable of elaborating neurites in a dose-dependent manner. these decreased cellular responses are probably due to an underglycosylation of the ngf receptor, since the effects of tunicamycin ar ... | 1985 | 2991546 |
| surveillance of enterovirus circulation in children communities. | in three children communities covered by a virological study enteroviruses could be isolated from 19.3% of the stool samples, 52.3% of the sewage samples and 9.7% of the samples taken from different objects and surfaces. the isolates belonged to 14 enterovirus serotypes, coxsackie viruses being predominant. there was a good concordance between the serotypes of enteroviruses shed in the stools and those isolated from the environment. the high epidemiological potential of enteroviruses calls for a ... | 1986 | 3008412 |
| prostaglandins, thromboxane and leukotriene in human follicular fluid. | the concentrations of prostaglandin e2 (pge2), prostaglandin f2 alpha (pgf2 alpha), thromboxane b2 (txb2) and leukotriene b4 (ltb4) were measured in the follicular fluid of 15 hyperstimulated human follicles. all the samples contained these eicosanoids. ova were recovered in six aspirates; the measured levels of prostanoids and ltb4 did not differ significantly from the levels of nine aspirates without ova. the presence of ltb4 in human follicular fluid suggests that the products of the lipoxyge ... | 1986 | 3022906 |
| [cloning of genes for proline biosynthesis in escherichia coli]. | restriction map of escherichia coli chromosome fragment (7.4 md) carrying proab genes was constructed. localization of proa and prob genes on the cloned chromosome fragment was determined by complementation test and the measuring of glutamylkinase activity (prob gene product). proa and prob genes were cloned separately on multicopy plasmids of alternative orientation and their expression being, probably, under the control of their own regulatory regions, studied. | 1986 | 3026898 |
| [cloning of genes for proline biosynthesis in escherichia coli]. | restriction map of escherichia coli chromosome fragment (7.4 md) carrying proab genes was constructed. localization of proa and prob genes on the cloned chromosome fragment was determined by complementation test and the measuring of glutamylkinase activity (prob gene product). proa and prob genes were cloned separately on multicopy plasmids of alternative orientation and their expression being, probably, under the control of their own regulatory regions, studied. | 1986 | 3026898 |
| establishment of a human t-cell hybridoma that produces human macrophage activating factor for superoxide production and translation of messenger rna of the factor in xenopus laevis oocyte. | human monoblast-like histiocytic lymphoma cell line u937 was induced by a macrophage activating factor for o2- production (maf-o) to produce o2- in response to phorbol myristate acetate stimulation. a maf-o-producing human t-cell hybridoma, f4-29-4, was established which was also found to produce macrophage activating factor for glucose consumption (maf-g) and colony stimulating factor (csf) when assayed against mouse bone marrow cells. maf-o could be successfully separated from csf but not from ... | 1987 | 3039355 |
| establishment of a human t-cell hybridoma that produces human macrophage activating factor for superoxide production and translation of messenger rna of the factor in xenopus laevis oocyte. | human monoblast-like histiocytic lymphoma cell line u937 was induced by a macrophage activating factor for o2- production (maf-o) to produce o2- in response to phorbol myristate acetate stimulation. a maf-o-producing human t-cell hybridoma, f4-29-4, was established which was also found to produce macrophage activating factor for glucose consumption (maf-g) and colony stimulating factor (csf) when assayed against mouse bone marrow cells. maf-o could be successfully separated from csf but not from ... | 1987 | 3039355 |
| protein c in human plasma determined by homogeneous enzyme immunoassay with use of a centrifugal analyzer. | we describe the simple and rapid enzyme immunoassay of protein c in human plasma with use of a cobas fara centrifugal analyzer. the antibody, labeled with horseradish peroxidase, is reacted with antigen (protein c) for 15 min. the peroxidase activity of the resulting antigen-antibody conjugate is measured at 500 nm for 5 min in the presence of excess h2o2, phenol, and 4-aminoantipyrine, as compared with that of free conjugates. results are calculated from a stored standard curve and expressed as ... | 1988 | 3046778 |
| anticonvulsant therapy in small animals. | this article includes an overview of the pathophysiology of seizures and a discussion of the mechanism of action of anticonvulsants based on the targeted physiologic processes. the commonly used anticonvulsants are classified and reviewed by chemical group. the concept, indications, and benefits of therapeutic monitoring of serum anticonvulsant concentrations are presented. the use of anticonvulsant agents for treatment of status epilepticus is addressed. | 1988 | 3064391 |
| the rate of dam-mediated dna adenine methylation in escherichia coli. | | 1988 | 3074008 |
| the ability of single sex infections of schistosoma japonicum to induce resistance to reinfection in mice. | in four experiments, mice harbouring an average 50, 76 or over 200 schistosoma japonicum female worms were not resistant when challenged six to eight weeks after infection. the female worms from these single sex infections were stunted and immature (average length 4.6 mm) and induced no overt pathology in the host. male worm burdens of 60, 135 or 140 also induced little or no resistance to challenge in the host. the males from these single sex infections were fully grown for their age (average l ... | 1986 | 3091678 |
| ontogenetic development of pancreatic thyrotropin-releasing hormone in human foetuses and in infants. | the ontogeny of thyrotropin-releasing hormone (trh) in pancreata of human foetuses from 15-36 weeks of gestation and of infants has been studied. trh was detectable in the pancreas of a 15 week old foetus; a progressive increase of pancreatic trh content was observed until the 34th week of gestation, whereas a progressive decrease was found in the late period of pregnancy and in 1 year old infants. in contrast, the pancreatic insulin content showed a progressive increase during the entire pregna ... | 1986 | 3092527 |
| membrane lipid composition of obligately and facultatively alkalophilic strains of bacillus spp. | the membrane lipids from two obligately and two facultatively alkalophilic strains of bacillus spp. were characterized in a comparative study that included b. subtilis. preparations of membrane lipids were made from ph 10.5-grown cells of all of the alkalophiles and from ph 7.5- or 7.0-grown cells of the two facultative strains and b. subtilis. the two obligate alkalophiles contained high ratios of membrane lipid to membrane protein, and the lipid fraction contained a high proportion of neutral ... | 1986 | 3093462 |
| lymphocyte response to klebsiella in ankylosing spondylitis. | the lymphocyte response of mononuclear cells (mnc) to klebsiella (klebs) was studied in ankylosing spondylitis (asp) and, for comparison, in patients convalescing from klebs infection and in hla-b27+ and b27- healthy blood donors. when large doses of various klebs cell envelope structures were used, mnc from all healthy blood donors were stimulated to produce lif but not to synthesize significant amounts of dna. in contrast, mnc from convalescent patients showed lif synthesis (but no significant ... | 1986 | 3100299 |
| ampicillin resistant gonorrhoea in hong kong. | | 1986 | 3102346 |
| evaluation of the 11co2 positron emission tomographic method for measuring brain ph. i. ph changes measured in states of altered pco2. | the 11co2 method for measuring local brain ph with positron emission tomography (pet) has been experimentally evaluated, testing the adequacy of the kinetic model and the ability of the method to measure changes in brain ph. plasma and tissue time/activity curves measured during and following continuous inhalation of 11co2 were fit with a kinetic model that includes effects of tissue ph, blood flow, and fixation of co2 into compounds other than dissolved gas and bicarbonate ions. for each of ten ... | 1987 | 3121647 |
| interaction of the bacillus subtilis phage phi 105 repressor dna: a genetic analysis. | the bacillus subtilis phage phi 105 repressor specifically recognizes a 14-bp operator sequence which does not exhibit 2-fold rotational symmetry. to facilitate a genetic analysis of this sequence-dependent dna binding a b. subtilis strain was constructed in which mutations affecting the phi 105 repressor-operator interaction cause a selectable phenotype, chloramphenicol resistance. after in vivo mutagenesis, we isolated and mapped 22 different mutations in the repressor coding sequence, 15 of w ... | 1988 | 3135184 |
| proteasomes (multi-protease complexes) as 20 s ring-shaped particles in a variety of eukaryotic cells. | latent multicatalytic protease complexes, named proteasomes, were purified to apparent homogeneity from various eukaryotic sources, such as human, rat, and chicken liver, xenopus laevis ovary, and yeast (saccharomyces cerevisiae), and their functional and structural properties were compared. they showed latency in breakdown of [methyl-3h]casein, but were greatly activated in various ways, such as by addition of polylysine. they all degraded three types of fluorogenic oligopeptides at the carboxy ... | 1988 | 3141402 |
| [elevated biliary excretion of t4--however, not of t3--in rats with nonthyroidal diseases (nti)]. | | 1988 | 3176822 |
| structural organization of the multienzyme complex of mammalian aminoacyl-trna synthetases. | the multienzyme complexes of mammalian aminoacyl-trna synthetases were purified from rat liver, rabbit liver, and rabbit reticulocytes according to the procedure slightly modified from kellermann et al. [kellermann, o., brevet, a., tonetti, h., & waller, j.-p. (1979) eur. j. biochem. 99, 541-550]. three forms of the synthetase complex with slightly different protein compositions were identified, suggesting a microheterogeneity of the synthetase complex. the hydrodynamic properties and the protei ... | 1988 | 3196691 |
| derivation of cells with lytic activity against fresh and cultured tumors from human bone marrow. | we studied the derivation of cells with lymphokine-activated killer (lak) cell activity from human bone marrow cultures. in these experiments, 26/26 specimens of human bone marrow obtained from sternum, iliac crest, rib, and humerus cultured with il-2 (1000 u/cc) under varying conditions were able to generate lak activity. lak activity obtained from bone marrow mononuclear cell cultures (bmmc) was observed only with exposure to il-2. peak lak activity against fresh melanomas and renal cell carci ... | 1988 | 3258943 |
| reflex changes in sympathetic nerve activity during mechanical ventilation with peep in sino-aortic denervated rats. | the aim of this study was to evaluate the role of cardiopulmonary receptors with vagal afferents in the reflex control of sympathetic nerve activity during mechanical ventilation with positive end-expiratory pressure (peep). experiments were performed on 17 chloralose-anaesthetized rats. changes in renal sympathetic nerve activity (rsna), heart rate and mean arterial pressure were studied at zero end-expiratory pressure (zeep) and at 5 and 10 cm h2o peep in intact animals (n = 8), after sino-aor ... | 1987 | 3296660 |
| fifteen-year experience with bloodstream isolates of coagulase-negative staphylococci in neonatal intensive care. | investigators worldwide, as well as the neonatologists and infection control team at our hospital, have reported that the incidence of coagulase-negative staphylococcal bacteremia in critically ill neonates has increased dramatically in recent years. to investigate these claims, we examined the results of all blood cultures obtained from 1970 to 1984 in our neonatal intensive care unit. throughout this study period, coagulase-negative staphylococci were prominent blood culture isolates (crude ov ... | 1988 | 3366867 |
| a special role of the parvocellular red nucleus in lesion-induced spontaneous tremor in monkeys. | the neural mechanisms underlying spontaneous tremor were investigated in monkeys. tremor-producing ventromedial tegmental (vmt) lesions involve at least three major neural elements. (1) parvocellular division of the red nucleus (rnpc); (2) cerebellothalamic fibers passing through the red nucleus, and, (3) nigrostriatal fibers. these three elements were destroyed stereotaxically in areas remote from the vmt area separately and/or in various combinations, and correlation between the site of lesion ... | 1988 | 3382517 |
| [neuromuscular junctions of the rat diaphragm in experimental hypoparathyroidism]. | the ultrastructure of neuro-muscular junctions in diaphragm muscle underwent certain changes in the course of experimental hypothyrosis (thermal destruction of a part of parathyroid gland). the most pronounced structural changes were observed in maximum hypocalcemia (7 days after the operation). there was a correlation between the pattern of a transmitter release and some parameters of vesicular apparatus (the number and volume distribution of synaptic vesicles). these effects suggest that hypop ... | 1988 | 3390602 |
| [a case of metastasis of gastric cancer to spermatic cord]. | a 63-year-old man underwent total gastrectomy and partial resection of the transverse colon for borrman iv gastric cancer on august 27, 1985. on august 8, 1986, 11 months after the operation, he visited our department with chief complaint of swelling of both groins. the spermatic cord was swollen to about 3 cm in diameter from both groins to inside of the scrotum and was hard as a club. the diagnosis of bilateral spermatic cord metastasis of gastric cancer was established and a biopsy was carrie ... | 1988 | 3400551 |
| avermectin toxicity in calves and an evaluation of picrotoxin as an antidote. | | 1988 | 3401165 |
| cancer. gene losses in human tumours. | | 1988 | 3419514 |
| common genetic pools between human populations. | a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ... | 1988 | 3422217 |
| potential radiopharmaceuticals for the detection of ocular melanoma. part ii. iodoquinoline derivatives and 67ga-citrate. | the uptake and distribution of several 4-(substituted amino)-iodoquinolines, 131i-labelled, and of 67ga-citrate were investigated in syrian golden hamsters with greene melanomas. because of its uptake in the melanoma (tumour/eye ratio 8.9), 4-(dimethylamino-ethylamino)-7-iodoquinoline (nm113) was selected for further investigations as a possible radiopharmaceutical for ocular scintigraphy when labelled with 123i. high uptake of nm113 turned out to be incidental and could not be favourably influe ... | 1986 | 3460807 |
| toxic effects of cyclosporine on the endocrine pancreas of wistar rats. | the widely used immunosuppressive drug cyclosporine exerts toxic effects on various parenchymal organs including the liver and kidney. this study was performed with the aim of testing whether cyclosporine also affects the endocrine pancreas. daily cyclosporine doses of 50 mg/kg body weight over 3 weeks in rats enhanced the serum bilirubin and creatinine concentrations, led to light-microscopic destruction in the liver and kidneys, and resulted in the development of an impaired glucose tolerance- ... | 1986 | 3510493 |
| court decisions streamline appeals process. | | 1986 | 3527937 |
| involvement of penicillin-binding protein 2 with other penicillin-binding proteins in lysis of escherichia coli by some beta-lactam antibiotics alone and in synergistic lytic effect of amdinocillin (mecillinam). | compared with cefotaxime, ceftazidime, moxalactam, and aztreonam, ceftriaxone produced the best lytic and bactericidal effects when each was added at about 10 times the mic to escherichia coli w7. when each of these antibiotics was added at its mic, only bacteriostasis occurred, but the simultaneous addition of amdinocillin (mecillinam) was synergistic in causing rapid lysis and bactericidal effects. induction of lysis of two e. coli mutants containing either a thermosensitive penicillin-binding ... | 1986 | 3545069 |
| topical minoxidil in early androgenetic alopecia. | a randomized, double-blind trial was conducted to determine the relative efficacy and safety of topical minoxidil solution for progressive male pattern baldness. one hundred fifty men with a discernible vertex balding spot were randomly assigned to treatment with either 2% topical minoxidil, 3% topical minoxidil, or placebo. after 4 months, patients receiving placebo were crossed over to 3% minoxidil. the study was concluded after 12 months of treatment, at which time 100 subjects were evaluable ... | 1987 | 3549807 |
| effect of altrenogest and lutalyse on parturition control, plasma progesterone, unconjugated estrogen and 13,14-dihydro-15-keto-prostaglandin f2 alpha in sows. | to investigate control of parturition time, 154 sows farrowing 220 litters at three locations were treated with altrenogest and lutalyse (pg). the four treatment groups were: 1) no treatment (control group); 2) an im injection of 15 mg of pg at 1000 on d 111, 112 or 113 of gestation (d 0 = first day of estrus and gestation); 3) altrenogest (20 mg x sow-1 x d-1) fed twice daily for 4 d starting on d 109, 110 or 111; and 4) altrenogest and an injection of pg at 1000 on the day after the last feedi ... | 1987 | 3610870 |
| bioavailability of phosphorus from defluorinated and dicalcium phosphates and phosphorus requirement of calves. | bioavailability of p from defluorinated phosphate and dicalcium phosphate and the p requirement were studied with 63 male holstein calves. a p depletion diet containing .08% total p on a dry matter basis was fed to all animals for 4 wk beginning at 6 wk of age and 61 kg weight. calves developed typical signs of p deficiency. the depletion period was followed by a 6-wk experimental period in which the same depletion diet was used as a control. phosphorus from each of the two sources was added to ... | 1987 | 3668046 |
| methylergometrine antagonizes 5 ht in the temporal artery. | methylergometrine, the main metabolite of methysergide, has 40-times greater 5 ht antagonistic potency than methysergide in isolated segments of human temporal artery. it is suggested that methylergometrine is the "active" drug when methysergide is used in migraine prophylaxis, and that the two compounds should be compared in prophylactic trials in migraine. | 1987 | 3691600 |
| inflammatory fibroid polyp of the stomach. | fourteen cases of inflammatory fibroid polyp of the stomach were studied in terms of immunohistochemistry and ultrastructure. they occurred as polypoid lesions in the antrum, except for two found in the body of the stomach. out of the 14 cases, two were found to be multiple and the remainder solitary. in all but one lesion, the mucosal layer was involved and six lesions were entirely localized within the mucosal layer, suggesting that inflammatory fibroid polyps of the stomach originate in the m ... | 1986 | 3716789 |