Publications

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enzymatic measurement of adenine and guanine (plus inosine) triphosphates and diphosphates in isolated cells and the mitochondrial matrix compartment obtained from rat liver. 1979114422
photoreversibly photochromic pigments in organisms: properties and role in biological light perception. 1979115051
the estimation of turnover of spermidine in anacystis nidulans. 1979115329
apoprotein aggregation on dissociation of flavin mononucleotide from flavodoxin [proceedings]. 1979119654
studies on the role of calcium ions in acetylene reduction (nitrogen fixation) by gloeocapsa sp. 1430/3 [proceedings]. 1979119655
effect of tryptophan analogues on lipid metabolism in the cyanobacterium anabaena cylindrica [proceedings]. 1979119656
energy transfer among the chromophores in phycocyanins measured by picosecond kinetics.energy-transfer processes in the algal light-harvesting proteins, the phycocyanins, have been studied by means of picosecond absorption spectroscopy. after excitation at 530 nm, the absorption at several wavelengths in the range 480--669 nm decayed with a short time constant (picosecond) and a long time constant (greater than 1 ns). for c-phycocyanin, energy transfer from the beta to the alpha subunits is interpreted as being a likely candidate for the short time constant; the long time constant ...1979115492
preparation of chlorophyll a by a nonchromatographic method. 1979115330
lipopolysaccharides of photosynthetic prokaryotes. 1979115382
accumulation of ppgpp and pppgpp during nitrogen deprivation of the cyanophyte anabaena cylindrica. 1979115718
cold-induced uncoupling of energy transfer between phycobilins and chlorophyll in anacystis nidulans: antagonistic effects of monovalent and divalent cations, and of high and low ph. 1979115721
mutants of anabaena strain ca altered in their ability to grow under nitrogen-fixing conditions.mutants of anabaena strain ca impaired in nitrogenase activity and growth on n2 were isolated and characterized. one mutant was selected for resistance to l-methionine-dl-sulfoximine, and others were selected for resistance to dl-7-azatryptophan or for requirements for combined nitrogen. the mutants varied in sensitivity of growth and nitrogenase activity to atmospheric 02. several of the mutants whose growth on n2 was impaired under aerobic conditions could grow and reduce acetylene at rates co ...1979115843
the role of the calvin cycle for anoxygenic co2 photoassimilation in anacystis nidulans. 1979115715
plasmids in two cyanobacterial strains. 1979115717
localization of polylysine inhibition in a photosystem i subchloroplast particle. 1979115467
soil stabilization by a prokaryotic desert crust: implications for precambrian land biota.a cyanophyte dominated mat, desert crust, forms the ground cover in areas measuring hundreds of square meters in utah and smaller patches in colorado. the algal mat shows stromatolitic features such as sediment trapping and accretion, a convoluted surface, and polygonal cracking. sand and clay particles are immobilized by a dense network of filaments of the two dominating cyanophyte species, microcoleus vaginatus and m. chthonoplastes, which secrete sheaths to which particles adhere. these micro ...1979116183
[morphologic heterogeneity under different conditions of living and destruction in anabaena variabilis cyanobacteria].the proportion between various morphological forms of the cyanobacterium anabaena variabilis was studied under different conditions of its growth and destruction. when the cells lost viability at the stationary phase of cultural growth under the optimal conditions of illumination (1500--2000 lx), in the dark or when nitrogen was deficient, the filaments became shorter (4 to 2.2 cells per filament on the average), the cells larger, 4.5x6 microns). under the conditions of nitrogen deficiency, the ...1979116116
herbicidins c and e, two new necleoside antibiotics.streptomyces saganonensis no. 4075 used for production of herbicidins a and b has also produced other two antibiotics, herbicidins c and e in submerged culture. isolation of the antibiotics was performed by adsorption on resinous adsorbent followed by elution with aqueous meoh and completed by silica-gel chromatography. physico-chemical characteriation revealed that herbicidins c and e are new nucleoside antibiotics having an adenine moiety in their structures. herbicidins c and e indicated inhi ...1979117003
preparation of an active, oxygen-evolving photosystem 2 particle from a blue-green alga. 1979116870
anaerobic hydrogenase activity in anacystis nidulans. h2-dependent photoreduction and related reactions.1. anaerobic hydrogenase activity in whole cells and cell-free preparations of h2-induced anacystis was studied both manometrically and spectrophotometrically in presence of physiological and artificial electron acceptors. 2. up to 90% of the activity measured in crude extracts were recovered in the chlorophyll-containing membrane fraction after centrifugation (144 000 x g, 3 h). 3. reduction of methyl viologen, diquat, ferredoxin, nitrite and nadp by the membranes was light dependent while oxid ...1979116679
an electrochemical approach to quantitation and characterization of metallothioneins. 1979118678
aerobic hydrogenase activity in anacystis nidulans. the oxyhydrogen reaction.1. the oxyhydrogen reaction of anacystis nidulans was studied manometrically and polarographically in whole cells and in cell-free preparations; the activity was found to be associated with the particulate fraction. 2. besides o2, the isolated membranes reduced artificial electron acceptors of positive redox potential; the reactions were unaffected by o2 levels less than 10--15%; aerobically the artificial acceptors were reduced simultaneously with o2. 3. h2-supported o2 uptake was inhibited by ...1979116680
[use of an immunofluorescence technic to detect unicellular cyanobacteria in natural bodies of water]. 1979119146
[immunostimulating activity of the lipopolysaccharides of blue-green algae].the whole cells of blue-gree algae and lipopolysaccharides isolated from these cells were shown to stimulate the production of macro-(mainly) and microglobulin antibodies in rabbits. the macro- and microphage indices in rabbits increased significantly after the injection of lps isolated from blue-green algae 24--48 hours before infecting the animals with a virulent y. pseudotuberculosis strain. besides, the inhibiting action of this strain on the migration of phagocytes to the site of infection ...1979117655
[blooming and destruction of cyanobacteria in the drainage bassin of the hydrogen sulfide spring of staraya matsesty].the growth of cyanobacteria belonging to the genera oscillatoria and anabaena (up to 2.1 x 10(7) filaments per 1 g of wet sample) was found in a water reservoir with a high content of sulfides (up to 9 mm) in staraya matsesta throughout the year. the spots of oscillatoria are located in the spring in more illuminated areas as compared to anabaena. in the spring, not only spots of actively growing cells were detected, but also accumulations of oscillatoria cells being destroyed (blue spots). wate ...1979119145
[extracellular metabolites and the satellite bacteria of blue-green algae]. 1979117285
the short-term effect of transfer from light to darkness on acetylene reduction (nitrogen fixation) by cultures of gloeocapsa sp. 1430/3 [proceedings]. 1979119659
composition and biological properties of lipopolysaccharides isolated from schizothrix calcicola (ag.) gomont (cyanobacteria).the most common cyanobacterium contaminating drinking water systems in southwestern pennsylvania is schizothrix calcicola. lipoplysaccharides (lps) were isolated from this species by hot phenol-water extraction. the polysaccharide moiety was composed of glucosamine, galactose, glucose, mannose, xylose and rhamnose. the lipid a part contained beta-hydroxylauric, myristic, pentadecanoic, palmitic, beta-hydroxypalmitic, stearic, oleic, and linoleic acids. in contrast to many lps isolated from enter ...1979119486
effects of simazine on the blue-green alga anacystis nidulans. 1979117857
effect of mercuric ion on the growth, photosynthesis, and nitrogenase activity of anabaena inaequalis.anabaena inaequalis was sensitive to mercuric ion (hg2+) in the ppb (nanogram per milliliter) range. growth was inhibited significantly at concentrations of metal ion as low as 2 ppb, and 100 ppb was required to inhibit photosynthesis and acetylene reduction. low levels of hg2+ stimulated acetylene reduction and photosynthesis. the lysis of vegetative cells was the primary action of mercuric ions, resulting in the inhibition of growth, photosynthesis, and nitrogenase activity. there was a linear ...1979119487
the cyanobacterial genome, its expression, and the control of that expression. 1979119432
synthesis of nitrogenase and heterocysts by anabaena sp. ca in the presence of high levels of ammonia.anabaena sp. ca fails to synthesize heterocysts and nitrogenase when grown with kno3 as the nitrogen source. by contrast, both heterocysts and proheterocysts are synthesized in nh4cl-containing media to a level nearly commensurate with cells grown in the absence of combined nitrogen. the growth rate of the organism in nh4cl-containing media was similar to that obtained with kno3 as the nitrogen source and was independent of the presence of n2 in the atmosphere. thus, our results indicate that th ...1979118162
effect of ions on the activity of the enzyme alkaline phosphatase from plectonema boryanum.alkaline phosphatase extracted from p. boryanum with lysozyme or polymyxin b treatment was used in a comparative study of cell bound and cell free enzyme. the effects of various ions on enzyme activity were tested. calcium was found to enhance activity to the greatest degree stimulating the cell bound alkaline phosphatase 100% and cell free enzyme four-fold. magnesium and potassium also stimulated the activity of cell bound and cell free enzyme. other ions were found to be inhibitory to varying ...1979120924
effects of microwaves on organisms. ptychodiscus brevis--gomphosphaeria aponina interactions.the two referenced organisms have been subjected to microwave irradiation (2,450 mhz cw). initial experiments indicated no statistically significant loss of cell numbers as a result of simple temperature rise (6 degrees c) or experimental manipulation. cell survival for p. brevis one week after irradiation was related to total energy absorbed, not power level used. over a 48 h period, a first-order decrease in cell numbers was observed. all cells were destroyed at a threshold level of 0.1 kj/cm3 ...1979120925
macromolecular synthesis in synchronized cultures of anacystis nidulans.synchronous culture of anacystis nidulans has been induced by the light-dark-light regimen. at various time intervals during synchronous growth, samples were pulsed with radioactive labels to determine phospholipid, protein, ribonucleic acid (rna), and deoxyribonucleic acid (dna) syntheses within the cell division cycle. a temporal order of protein, rna, and dna syntheses occurred within the cell division cycle, whereas phospholipid was characteristically synthesized during midcycle (during cell ...1979115841
properties of allophycocyanin ii and its alpha- and beta-subunits from the thermophilic blue--green alga mastigocladus laminosus.purified allophycocyanin ii and its subunits have been examined with respect to spectroscopic properties, sedimentation, reconstitution and isoelectric behaviour. in 0.02m-potassium phosphate buffer, ph8.0, and at a concentration of 0.25mg/ml, allophycocyanin ii and its alpha- and beta-subunits show visible absorption maxima at 650, 615 and 615nm respectively, whereas the fluorescence emission maxima were determined to be at 662, 640 and 630nm respectively. the absorption difference spectrum (di ...1979117797
the effect of nickel on the growth, photosynthesis, and nitrogenase activity of anabaena inaequalis.anabaena inaequalis was sensitive to nickel ion in the order of decreasing sensitivity of growth, photosynthesis, and acetylene reduction. at a culture density of 9 x 10(4) cells per millilitre, growth after 12 days was completely inhibited by 0.125 ppm (microgram/ml) ni2+. nickel caused the increase of both the lag phase of growth and the culture doubling time, and caused the retardation phase to be sooner. photosynthesis and acetylene reduction were completely inhibited by 10 and 20 ppm ni2+, ...1979120221
influence of pesticides on the growth of cyanobacteria.two unicellular and two filamentous cyanobacteria (blue-green algae) were exposed under conditions optimal for photoautotrophic growth to eleven pesticides. low concentrations (0.01 to 5 ppm) of diuron, atrazine, and paraquat inhibited growth. with mcpa, mcpp, 2,4-d, milstem and ethrel, marked inhibitory effects were achieved only at concentrations above 100 ppm. growth was inhibited by glyphosate, ddt, and thiram at intermediate concentrations. in some cases, the effective concentration of the ...1979120640
phycobilisomes from the blue-green algae aphanizomenon flos-aquae and anabaena variabilis.phycobilisomes (pbs) were isolated from aphanizomenon flos-aquae and anabaena variabilis. the absorption spectra and second derivative of the absorption spectra of isolated pbs indicate the presence of phycoerythrocyanin, and allophycocyanin. the fluorescence spectra of pbs were measured at room temperature and -196 degrees c. undamaged pbs have the principal fluorescence maximum in the region of 660 nm at room temperature and in the region of 685-690 nm at -196 degrees. when the pbs were heated ...1979121545
structure and evolution of photosynthetic accessory pigment systems with special reference to phycobiliproteins. 1979122171
reaction of freshwater phyto- and zooplankton to pesticides.the reaction of plankton to the toxic effect of pesticides is considered at four levels: the cell, the organism, the population, and the biocenosis. the basis for the methodological approach to evaluation of response at the cell level was the principle of phase reactions. depending on concentration, the pesticides may either suppress or stimulate the plankton organisms. temperature may be decisive intoxic effects (pesticides are effective in a range of 15 to 25 degrees c). most pesticides having ...1979121548
evolutionary implications of different types of microbial enzymology for l-tyrosine biosynthesis.several patterns of enzymology for l-tyrosine biosynthesis exist in modern microorganisms, each differing in the apparent degree of regulatory efficiency. the extent of pathway evolution in a particular organism may reflect the relative selective pressure for regulation encountered in different ecological niches.1975123637
anacystis nidulans mutants resistant to aromatic amino acid analogues.three classes of mutants of anacystis nidulans were selected on the basis of resistance to fluorophenylalanine and 2-amino-3-phenylbutanoic acid. the most frequent type exhibited dahp synthetase (7-phospho-2-keto-3-deoxy-d-arabino-heptonate-d-erythrose-4-phosphate-lyase [pyruvate phosphorylating], ec 4.1.2.15) activity identical to that of the parental strain. the second type was characterized by extremely low levels of the activity. the third type had a dahp synthetase showing decreased sensiti ...1975125268
formation of benzoic acid and p-hydroxybenzoic acid in the blue green alga anacystis nidulans: a thylakoid-bound enzyme complex analogous to the chloroplast system.the photosynthetic procaryote anacystis nidulans converts l-phenylalanine and l-tyrosine into benzoic acid and p-hydroxybenzoic acid, respectively. results obtained with thylakoid fractions support the hypothesis that the reaction sequence is catalyzed by thylakoid-bound enzyme complexes consisting of phenylalanine ammonia-lyase and benzoate synthase of tyrosine ammonia-lyase and p-hydroxybenzoate synthase, respectively. btoh complexes do not accept phenylacetic acids as substrates, and cinnamic ...1976138288
ancient locomotion: prokaryotic motility systems. 1978122508
[activity of aminotransferases in the blue green alga anacystis nidulans].the blue-green alga anacystis nidulans (strain l 1402-1) was grown at +37 degrees c in air (0.03 vol% co2 and in air enriched with 3.0 vol% co2. the effects of several inhibitors on the activity of aminotransferases, 14co2 fixation and radioactive photosynthetic products of anacystis were studied. no serine-pyruvate aminotransferase activity could be found in 10-2 m isonicotinyl hydrazide (inh); under the influence of this inhibitor aspartate and alanine aminotransferase were decreased about 49% ...1976134584
[amino acid content in some blue-green algae of moldavia]. 1976133734
[molecular nitrogen fixation in eutrophic beloye ozero].the rate of production and mineralization of organic matter and the rate of fixation of molecular nitrogen were assayed in th eutrophic lake beloye (near moscow) at the end of summer stagnation (1976). the content of hydrogen sulphide reached 6.8 mg per litre which resulted in a change of the ecological environment in the lake. the fixation of molecular nitrogen in water layers was characterized by three maxima: 5.0 mcg n per litre per 4 hours (surface); 4.2 mcg n per litre per 4 hours (metalimn ...1978148551
chromophore content of c-phycoerythrin from various cyanobacteria.the molar extinction coefficient for phycoerythrobilin (l a) was calculated by two independent methods. it is different from that of the cleaved chromophore, phycobiliviolin (2). by unfolding with urea or tryptic digestion, the chromophore absorption of c-phycoerythrin (pe) was determined free of any protein influence. the chromophore content of pe from various cyanobacteria was determined with these data to be either 5 or 6, depending on the organism. this corresponds to a chromophore distribut ...1977146359
isolation and characterization of a coupling factor i atpase of the thermophilic blue-green alga (cyanobacterium) mastigocladus laminosus. 1979157885
preparation of [u-14c]-labelled glycogen, maltosaccharides, maltose, and d-glucose by photoassimilation of 14co2 in anacystis nidulans and selective enzymic degradation. 1977143346
[skin lesions caused by contact with blue-green algae]. 1979156475
uptake of the nitrogen fixing blue-green alga gloeocapsa by plant protoplasts. 1977144498
cyanobacterial ribonucleic acid polymerases recognize lambda promoters.we compared the initiation specificities in vitro of deoxyribonucleic acid-dependent ribonucleic acid polymerases purified from two cyanobacteria, fremyella diplosiphon and anacystis nidulans, and from escherichia coli. a restriction fragment made from lambda deoxyribonucleic acid was used as a template. the cyanobacterial and e. coli ribonucleic acid polymerases recognized the same lambda promoters but exhibited different sensitivities to the inhibitor heparin, suggsesting differences in the st ...1979159286
reconstitution of cyanobacterial photophosphorylation by a latent ca2+-atpase. 1979159701
the purification and properties of superoxide dismutase from a blue-green alga.soluble extracts of plectonema boryanum have been shown to contain a single, electrophoretically distinct, superoxide dismutase. the enzyme has been isolated and has been found to be an iron-containing enzyme similar to that described from the periplasm of escherichia coli. it contains 1 fe3+/mole of enzyme. the molecular weight was approximately 36 500, and the enzyme appeared to be composed of two subunits of equal size joined by non-covalent interactions. esr data are presented, as are the r ...1975164232
[interferometric studies of the dynamics of hydration and dry matter content during light-dependent germination of the anabaena variabilis kützing akinetes].calculations following interference-microscopical measurements performed on akinetes (a), heterocyts (h), and "vegetative" cells (f) of the cyanobacterium (blue-green alga) anabaena variabilis resulted in significant higher values of mean absolute dry matter content of the akinetes (2.06 . 10(-10) g; as compared to 0.46 . 10(-10) g and 0.31 . 10(-10) g for h and f, respectively). tthe water content of these resting cells (63%) was significantly lower than in the other two types of cells (h: 85%, ...1979120999
biochemical studies on sporulation in blue-green algae. i. glycogen accumulation.glycogen accumulation in vegetative cells of anabaena sp. is demonstrated to be a light-dependent process. no glycogen accumulation is found in dark or in cultures supplemented with 10(-5) m dcmu in light. large quantities of glycogen accumulate in cells undergoing sporulation and the amount increased with the onset of maturation of spores.1979121000
the occurrence of the pyruvate dehydrogenase complex and the pyruvate-ferredoxin oxidoreductase in blue-green bacteria. 1975165997
citrate synthase from blue-green bacteria. 1975165998
immunological comparison of ferredoxins. 1975166874
[reduction paths of reaction centers of photosystem i of photosynthesis].the behaviour of esr signal i in blue-green algae and chloroplasts is considered under various illumination and with various previous treatment. it is shown that the main process determining the kinetics of p700 plus reduction is the reaction with reduced components of the electron transfer chain between the two photosystems. if the accumulation of the products is inhibited by any way, then the reduction rate depends on the reactions with reducing agents in the incubation medium accumulated unde ...1975167869
temporal genetic mapping in the blue-green alga anacystis nidulans using ethyl methanesulphonate.cultures of the blue-green alga anacytis nidulans were synchronized with respect to dna synthesis as well as cell division. application of ethyl methanesulphonate at different stages of replication resulted in a peak of mutation frequency for different genetic markers; this peak can be accounted for in terms of the involvement of repair processes. a temporal map of 19 markers has been constructed by this method. comparison of gene position obtained by temporal mapping indicates that either bidir ...1975168309
lactate dehydrogenases in cyanobacteria.nad-linked lactate dehydrogenases specific for the d- and l-lactate have been demonstrated in a number of strains of unicellular cyanobacteria. the d-lactate dehydrogenase of one strain (synechococcus 6716) was partially purified and its properties were studied. the enzyme has a molecular weight of ca. 115000-120000, is highly specific, autooxidizable, and susceptible to inhibition by iodoacetamide, oxamate and atp. the possible physiological functions of the enzyme in the metabolism of the o ...1975168830
in vitro repair of uv-or x-irradiated bacteriophage t4 dna by extract from blue-green alga anacystis nidulans.the cell-free extract from blue-green alga anacystis nidulans contains enzymatic activities which repair in vitro transforming dna of bacteriophage t4 damaged by uv light or x-rays. the repair effect of the extract was observed with double-stranded irradiated dna but not with denatured irradiated dna. the level of restoration of the transforming activity depends on the protein concentration in the reaction mixture and on the dose of irradiation. a fraction of dna lesions induced by x-rays is rep ...1975169464
formation in the dark, of virus-induced deoxyribonuclease activity in anacystis nidulans, an obligate photoautotroph.in anacystis nidulans, upon infection with cyanophage as-1, after a lag period of 1 h the level of deoxyribonuclease (dnase) activity increaded rapidly up to 15- to 20-fold in 4 to 5 h in the light. in contrast, the ribonuclease and phosphomonoesterase activities increased significantly only 4 to 5 h after infection, i.e. as late as 1 h prior to lysis. in complete darkness, the nuclease levels remained unaltered. however, when the infected cells were exposed to light for 1 or 2 h after infection ...1976177400
[effect of the detergent metaupon on replication of various phages].as several other surfactants do, the detergent metaupon acts on the multiplication of bacteriophages. we investigated the influence of metaupon on the phages phi and lambda, the cyanophage lpp-1, and the rna-phages f 2, m 12, and q beta by means of the agar diffusion test, pour plate test, adsorption test, and one-step growth test. the action of metaupon on the free phages was also tested. metaupon inhibits the formation of plaques by the phages with exception of lambda. with the phages f 2 and ...1979161679
properties of the low-temperature photosystem i primary reaction in the p-700-chlorophyll alpha-protein.the photosystem i primary reaction, as measured by electron paramagnetic resonance changes of p-700 and a bound iron-sulfur center, has been studied at 15 degrees k in p-700-chlorophyll alpha-protein complexes isolated from a blue-green alga. one complex, prepared with sodium dodecyl sulfate shows p-700 photooxidation only at 300 degrees k, whereas a second complex, prepared with triton x-100, is photochemically active at 15 degrees k as well as at 300 degrees k. analysis of these two preparatio ...1976179589
effect of restriction endonucleases on infectivity of rous sarcoma virus dna. 1976180429
spin lattice relaxation and exchange interaction in a 2-iron, 2-sulphur protein.a two-iron-two-sulphur non-haem iron protein, the ferredoxin from spirulina maxima, has been studied by means of electron paramagnetic resonance (epr) in the range where the spectrum loses resolution with increasing temperature. the spin-lattice relaxation times were deduced from linewidths measured by spectral simulation and their variation as a function of temperature is interpreted in terms of an orbach mechanism. on this basis, the exchange integral between the two iron atoms, assuming as an ...1976181065
distribution of thioredoxins in cyanobacteria.the presence of thioredoxin was demonstrated in 20 strains of cyanobacteria as well as in one phototrophic bacterium rhodopseudomonas sulfidophila and in thiobacillus denitrificans. thioredoxin activity was not found in cyanophora paradoxa and in porphyridium cruentum using the thioredoxin-dependent paps-sulfotransferase activity from synechococcus 6301 as assay system.1979161845
crystalline structure of the gas vesicle wall from anabaena flos-aquae. 1976184286
uptake of iron by gomphosphaeria aponina, a possible control organism for the florida red tide pytochodiscus brevis.the assimilation of iron, a growth-limiting metal ion of the cytotoxic marine cyanobacterium, gomphosphaeria aponina, has been examined in both static and steady-state cultures using 59fe (iii). uptake of iron by cells followed first-order kinetics, and biphasic (absorption and uptake) behavior was observed as suggested by noted differences between cultures incubated in the light and in the dark. iron removal in illuminated cultures was rapid, occurring at rates comparable to exponential growth ...1979162148
properties of the primary electron acceptor complex of photosystem i in the blue green alga chlorogloea fritschii. 1976178303
resonance raman studies of a c type algal cytochrome. deuterium shifts and a comparison with mammalian cytochrome c.a c type cytochrome isolated from synechococcus lividus grown on water and 2h2o media, has been studied by resonance raman spectroscopy. the spectra were taken on the oxidized and reduced protein with excitation within the soret band at 441.6 nm to determine whether individual resonance raman bands of the heme shift upon deuterium substitution and also to provide a comparison with the spectra of horse heart cytochrome c. some of the shifts observed with the deuterated heme c are larger than the ...1976182237
the roles of c-type cytochromes in algal photosynthesis. extraction from algae of a cytochrome similar to higher plant cytochrome f.a membrane-bound cytochrome resembling higher plant cytochrome f in many respects has been extracted from the algae chlamydomonas. euglena and anacystis, and partially purified. the spectra of the cytochromes from chlamydomonas and euglena are virtually identical to that of parsley cytochrome f, with alpha-band maxima near 554 nm, very asymmetrical beta-bands, and gamma-band maxima at 421 nm. the cytochrome from anacystis had alpha and gamma-bands both shifted to slightly longer wavelengths. the ...1977190006
electron paramagentic resonance studies of photosynthetic electron transport: photoreduction of ferredoxinand membrane-bound iron-sulfur centers.electron paramagnetic resonance spectrometry was used to investigate, at physiological temperatures, light-induced electron transport from membrane-bound iron-sulfur components (bound ferredoxin) to soluble ferredoxin and nadp(+) in membrane fragments (from the blue-green alga, nostoc muscorum) that had high rates of electron transport from water to nadp(+) and from an artificial electron donor, reduced dichlorophenolindophenol (dciph(2)) to nadp(+). illumination at 20 degrees resulted in the ph ...1977198808
kinetics of light-dark co2 fixation and glucose assimilation by aphanocapsa 6714.cells of aphanocapsa 6714 were subjected to alternating ligh-dark periods (flashing-light experiments). the corresponding activation (in the light) and inactivation (in the dark) of the reductive pentose cycle was measured, in vivo, from initial rates of 14co2 incorporation and also by changes in the total concentration of 14c and 32p in soluble metabolites. two principle sites of metabolic regulation were detected: (i) co2 fixation was inactivated 15 to 20 s after removal of the light source, b ...1976185198
epr spectra of photosystem i constituents in heterocyst preparations from anabaena cylindrica. 1976186323
the ultrastructure of spirulina platensis in relation to temperature and light intensity.the ultrastructure of spirulina platensis, a cyanobacterium with a helical morphology, has been studied in relation to temperature and light intensity. an increase in temperature gives rise to a more tightly coiled trichome, an increase in sheath material formation and a decrease in cyanophycin (above 17 degrees c) and polyglucan (above 20 degrees c) granule concentration. an increase in light intensity leads to an increase in gas vesicle concentration while the phycobilisome content decreases. ...1979233414
superoxide dismutases from a blue-green alga, plectonema boryanum.iron-containing and manganese-containing superoxide dismutases were found in plectonema boryanum. the mn-enzyme occupies about 10% of total activity. the fe-enzyme was purified to near homogeneity. it contains 2 atoms of iron per mol. its molecular weight is 41,700 and it is composed of two subunits of identical molecular weight without disulfide linkage. amino acid composition is presented. electron paramagnetic resonance spectrum revealed that iron occurs in a high spin ferric form and in some ...1975164453
studies on growth, nitrogen fixation and protein synthesis in a blue-green alga calothrix sp. 1975164760
polyphosphate-deficient mutants of anacystis nidulans.polyphosphate-deficient mutants of anacystis nidulans have been isolated by either ethyl methanesulfonate (ems) or n-methyl nitrosoguanidine (ntg) mutagenesis and penicillin-enrichment techniques. mutagenised stock was preincubated in a medium lacking sulfate, then transferred to a phosphate-lacking medium before penicillin treatment. many single-colony isolates, in contrast to wild-type, show little growth in absence of phosphate, and have altered polyphosphate, and have altered polyphosphate k ...1978206328
the fine structure of a tri-lamellar body in various species of anabaena. 1976184493
[cytomorphology of the lipid inclusions of caryophanon during its growth on an agarized medium].the cytomorphology of lipid inclusions of caryophanon latum and caryophanon tenue was studied in the course of their growth on a solid medium. the paper presents a detailed scheme and microphotographs showing cytomorphological changes in the inclusions of the cultures under study. staining of the preparations with the lipophilic dye sudan black b revealed two types of sudanophilic granules which varied in their location within trichomes, the time at which they appeared in bacteria, and their beh ...1978212673
two plant-type ferredoxins in light-grown and dark-grown cells of the blue-green bacterium nostoc strain mac. 1975236956
glutamate synthase in blue-green algae. 1975236957
glucose dehydrogenase of chlorogloeopsis fritschii. 1975236958
[electron microscopic data on the mesosomelike and myelinlike structures of blue-green algae].the mesosome-like structures of blue-green algae were studied by freeze-etching without preliminary fixation and, in parallel, by a technique of ultrathin sections. these structures were found mainly in synechococcus elongatus and synechocystis aquatilis, less often, in anacystis nidulans and anabaena variabilis, and were not detected in plectonema boryanum. the following types of membrane structures were encountered: (1) formation consisting of three- and five-layered membranes and resembling t ...1978212674
multiple functions of thioredoxins.reduced thioredoxins from microbial and plant cells, both of cytoplasmic or chloroplast origin, are interchangeable in stimulating such diverse enzyme activities as ribonucleoside diphosphate reductase (e. coli), paps sulfotransferase (synechococcus), and fructose-1,6-bis-phosphatase (from spinach) in vitro. it is suggested that reduced thioredoxins are unspecific, multifunctional cellular proteins while in contrast the oxidized froms require specific enzymes for their reduction.1978212888
the origin of antitumour activity in sea anemones. 1975240615
isolation of photosynthetic catalysts from cyanobacteria.methods are described for the isolation of ferredoxins i and ii, cytochrome c-553, cytochrome f, cytochrome c-550 and plastocyanin from large quantities of various cyanobacteria. the amino acid composition of cytochrome c-550 is reported. there is a variation in the relative amounts of these proteins in different batches of cells which may relate to the nutritional status of the organisms.1979216399
an iron-containing superoxide dismutase from anacystis nidulans.superoxide dismutase (sod) was isolated and purified from anacystis nidulans to near electrophoretic homogeneity. the enzyme has a molecular weight of 37,500, as determined by gel filtration and sds-gel electrophoresis. the enzyme molecule consists of two subunits of identical molecular weight. proton-induced x-ray elemental analysis (pixe) showed that the sod of a. nidulans is an iron-containing enzyme; the fe:enzyme mol ratio was found to be 1. the epr spectra indicated that the active center ...1979222743
the dark respiration of anacystis nidulans. production of hcn from histidine and oxidation of basic amino acids.the basic amino acids, l-arginine, l-lysine, lo-irnithine, and to a lesser extent l-histidine, strongly stimulate the o2 uptake of cell suspensions of the blue-green alga or cyanobacterium anacystis nidulans. in the case of l-histidine, the extra o2 consumption is associated with the formation in vivo of small amounts of hcn, particularly in an atmosphere of o2. the enzyme responsible for both the stimulated o2 uptake with the basic amino acids and the formation of hcn from histidine has been is ...1979223652
paracrystalline inclusions in various isolates of the blue-green bacteria nostoc and anabaena.a number of different crystalline inclusions were observed in various isolates of anabaena and nostoc. membrane-limited crystalline bodies were observed in 7 of 20 isolates of anabaena and 19 of 29 isolates of nostoc. these are spherical, single membrane-limited bodies from 0.6 to 0.1 micron in diameter. in most of the isolates they contained needle-like crystals 20 a in thickness and up to 80 nm in length. in 9 of the isolates the inclusions contained granular and fibrillar material. the number ...1978223816
a new specific endonuclease from anabaena variabilis. 1979225204
regulation of phosphate accumulation in the unicellular cyanobacterium synechococcus.the phosphorus contents of acid-soluble pools, lipid, ribonucleic acid, and acid-insoluble polyphosphate were lowered in synechococcus in proportion to the reduction in growth rate in phosphate-limited but not in nitrate-limited continuous culture. phosphorus in these cell fractions was lost proportionately during progressive phosphate starvation of batch cultures. acid-insoluble polyphosphate was always present in all cultural conditions to about 10% of total cell phosphorus and did not turn ov ...1979227842
epr spectra of photosystem i and other iron protein components in intact cells of cyanobacteria.electron paramagnetic resonance (epr) spectra were recorded of whole filaments of the cyanobacteria nostoc muscorum and anabaena cylindrica. signals due to manganese were removed by freezing and thawing the cells in edta. epr spectra were assigned on the basis of their g values, linewidths, temperature dependence and response to dithionite and light treatments. the principal components identified were: (i) rhombic fe3+ (signal at g = 4.3), probably a soluble storage form of iron; (ii) iron-sulfu ...1979228708
temperature dependence of the electronic spin-lattice relaxation time in a 2-iron-2-sulfur protein.the ferredoxins are characterized by a strong temperature dependence of the electronic spin-lattice relaxation time t1. the measurement of this dependence above the liquid nitrogen temperature has been presented in earlier work [1] for the 2-iron-2-sulfur ferredoxin of the blue green alga spirulina maxima. the different relaxation mechanisms which could be efficient in this range were briefly discussed. in the present paper, we extend the measurement of the temperature dependence of t1 to the lo ...1979228745
effect of nitrogen starvation on the level of adenosine 3',5'-monophosphate in anabaena variabilis.low levels of adenosine 3',5'-monophosphate (cyclic amp) were detected in the cyanobacterium anabaena variabilis using a protein binding assay and two radioisotopic labelling methods. the basal concentration of intracellular cyclic amp ranged from 0.27 pmol/mg protein in a. variabilis kutz grown under heterotrophic conditions to 1.0--2.7 pmol/mg protein in a. variabilis strain 377 grown autotrophically. extracellular cyclic amp was found to comprise as much as 90% of the total cyclic amp in rapi ...1979228756
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