Publications

activation of the estrogen receptor from n-nitrosomethylurea-induced rat mammary tumors.	the activation of the estrogen receptor (er) from n-nitrosomethylurea (nmu)-induced rat mammary tumors was studied in vitro. the activation of the receptor induced by heating of the cytosol containing occupied er was measured by a 3-4-fold increase of receptor binding to nuclei in comparison with the nuclear binding of the nonactivated er. the activation of the er was further shown by alteration of the elution profile from deae-cellulose. a shift of the receptor peak from 234 mm (peak ii, nonact ...	1988	3347053
the chemical peel.	the spectrum of chemical peeling encompasses a diverse group of peeling agents with the ability to produce a wide range of cutaneous burns. the most spectacular peels are produced by the phenol formulas. the physician, however, is restricted in the number of potential candidates as determined by skin color and potential toxicity. while not as impressive, trichloroacetic acid peels are an excellent modality for treatment of actinic keratoses, actinic dermatitis and lentigoes. improvement of rhyti ...	2012	3328645
the radiological identification of tumours in children.	the diagnostic imaging procedures now available for the identification of tumours are many. much of the information gained from one imaging procedure is simply duplicated by another without the addition of new information. this is wasteful of resources. an understanding of the advantages and limitations of each technique is essential for the selection of the best diagnostic pathways. these are briefly described and followed by a brief discussion of the role of radiology in the protocols for the ...	2012	3322552
effects of endotoxemia on pulmonary vascular resistances in unanesthetized sheep.	ten experiments were conducted on nine sheep to determine the effects of endotoxemia (1.0 microgram/kg iv over 15 min) on the vascular resistances of two segments of the pulmonary circulation. the first segment (s1) was from the main pulmonary artery to the site in the pulmonary veins corresponding to the pressure measured with a deflated and wedged 7-fr swan-ganz catheter. the second segment (s2) was from the wedge pressure measurement site to the left atrium. endotoxemia caused both pulmonary ...	1987	3308813
recent developments in our knowledge of steroids.		2012	3306737
cytotoxic escherichia coli.		1987	3305326
[subcutaneous rupture of tendons and muscles of the extremities].	under study there were 278 patients with 285 subcutaneous ruptures of tendons and muscles of the upper and lower extremities. the tendon of the long head of the humeral biceps muscle and achilles tendon are most liable to subcutaneous ruptures (123 patients--43.2% and 97 patients--34% respectively). pathohistological examinations have shown that subcutaneous ruptures of tendons and muscles usually take place against the background of dystrophic alterations reducing their mechanical strength. the ...	2012	3303625
the role of the neuroradiologist in vascular disorders involving the orbit.	ct and mri have become imaging techniques of great value in the diagnosis of vascular orbital lesions. intraorbital vascular lesions, vascular tumors, intracranial aneurysms, carotid cavernous fistulas, and tolosa-hunt syndrome are discussed in detail. they illustrate how the accuracy of ct and mri in evaluating orbital and intracranial masses has focused the role of angiography in exophthalmos and ophthalmoplegia on detection and assessment of those vascular lesions that may require surgical in ...	2012	3299479
the effect of progressive relaxation on valsalva response in healthy adults.	the purpose of the study was to determine if the tensing portion of progressive relaxation initiates the valsalva response in order to establish the safety of the procedure for acutely ill cardiovascular patients. sixty healthy adult volunteers performed fist, chest, and abdominal tensing and relaxation while their heart rate was continuously monitored. occurrence of the valsalva response was determined by dividing peak tachycardia during tensing by maximum bradycardia during releasing the tensi ...	2012	3296020
[biochemistry of glycoconjugates in the studies of cancer metastasis].		2011	3295999
[biochemistry of glycoconjugates in the studies of cancer metastasis].		2011	3295999
methylene blue: a reliable and practical marker for validating compliance on the dst.	assurance of compliance (ingestion of dexamethasone) is crucial for interpreting plasma cortisol results on the dst. in this double-blind study of 13 subjects, methylene blue (mb) 50 mg was combined with dexamethasone 1 mg in single capsules, and the resulting blue-green urinary color after ingestion was found to reliably validate compliance in 100% of patients and controls. the addition of mb did not influence dst results (i.e., plasma cortisol, plasma dexamethasone). adding mb to dexamethasone ...	2012	3294814
characterization of the copper-thiolate cluster in yeast metallothionein and two truncated mutants.	cu-metallothionein was purified from saccharomyces cerevisiae harboring plasmids containing mutated cup1 metallothionein genes resulting in deletions at the carboxy-terminal end of the polypeptide. the truncated polypeptides are recovered as polypeptides of 35 and 48 residues in length. the cu-s cluster in the wild-type metallothionein and the two truncates were characterized. the truncated proteins, designated t35 and t48, contain 4 and 2 fewer cysteinyl residues, respectively, compared to the ...	1988	3283130
differential regulation of amyloid-beta-protein mrna expression within hippocampal neuronal subpopulations in alzheimer disease.	we have mapped the neuroanatomical distribution of amyloid-beta-protein mrna within neuronal subpopulations of the hippocampal formation in the cynomolgus monkey (macaca fascicularis), normal aged human, and patients with alzheimer disease. amyloid-beta-protein mrna appears to be expressed in all hippocampal neurons, but at different levels of abundance. in the central nervous system of monkey and normal aged human, image analysis shows that neurons of the dentate gyrus and cornu ammonis fields ...	1988	3277189
collagen fiber alignment in the human preauricular dermis before and after rhytidectomy--a scanning electron microscopy study.	the microarchitecture of collagen fibers in preauricular dermis specimens that were obtained during two-phase rhytidectomy were visualized and recorded using the scanning electron microscope. examination of photomicrographs revealed that both spatial arrangement and degree of convolution of the collagen fibers were completely different in the specimens of the two phases. the results are interpreted in terms of the viscoelastic properties of the skin.	1986	3273035
complications of liquid nitrogen cryosurgery for verrucae over bony prominences.	liquid nitrogen cryosurgery is the most frequently used surgical treatment for verrucae. its success rate is high and complications are rare. however, liquid nitrogen should be used with great care over bony prominences where skin and subcutaneous tissues are thin. we present 3 patients in whom the treatment of simple warts over bony prominences by liquid nitrogen resulted in full-thickness loss of skin. in 2 of them, extensor tendons of fingers were exposed and had to be covered with flaps. we ...	2012	3273024
oral abnormalities in the ellis-van creveld syndrome: case report.		2012	3272958
leao's spreading depression in the hippocampus.		2008	3259786
colorimetric determination of amoxycillin in presence of penicillins and cephalosporins.		2012	3255746
in situ optical spectroscopy of some systems of biological interest.	monitoring the optical absorption or emission spectrum of a condensed phase sample offers information about the supramolecular assembly, packing effects and other features characteristic of the phase that would be missed when one studies solution-state spectra. we have used the technique of photoacoustic spectroscopy to study intact biological specimens, such as algae, parasite cells and the eye lens. such a study has offered information about the status of endogenous hemin in plasmodium cells a ...	1988	3242637
ideals and reality in actual regulatory experience.		2011	3238057
[toxicologic properties and the nature of the combined effect of a orthomenthane hydrocarbon mixture].		1988	3234846
[hemodynamics in the early postoperative period after thoracotomy and lung resection].	to evaluate how necessary and helpful invasive hemodynamic measurements after thoracic surgery are we studied 25 patients after pneumonectomies (n = 5), lobectomies and bilobectomies (n = 10), partial lobectomies (n = 6) and thoracotomies without lung resection (n = 4). measurements were taken between half an hour and eighteen hours after closure of the rib cage using a preoperatively introduced swan-ganz-catheter. pulmonary resistance was elevated in 12 out of 17 cases. 6 out of 25 patients had ...	2011	3232137
pregnancy rate and foetal mortality in aleutian disease virus infected mink.		1988	3223478
[occurrence and clinical picture of parasitic diseases among patients of the infectious disease clinic, medical academy, in białystok 1975-1986].		2011	3218251
when is serum albumin worth measuring?		2011	3214132
gardner's syndrome presenting with proptosis.		2011	3209939
alpha-lactalbumin production by rat mammary gland: prepartum emergence of resistance to glucocorticoids and progestins.	explants of mammary glands from pregnant or lactating rats were shown to differ markedly in their response to glucocorticoid and progestin in terms of alpha-lactalbumin production. tissue from day 14 pregnant rats had low initial tissue levels and basal production of alpha-lactalbumin, and showed a dose-biphasic response to glucocorticoids, with significantly higher production at 3 nm than 300 nm ru26988; the progestin org 2058 inhibited production. in explants from lactating rats (day 1 and day ...	1988	3208993
nursing research in cardiovascular risk reduction.		2011	3203349
function of the striated anal sphincter during straining in control subjects and constipated patients with a radiologically normal rectum or idiopathic megacolon.	the function of the striated anal sphincter during defaecation straining was recorded by manometry and electromyography (e.m.g.) in 31 constipated patients who were unable to expel a water-filled rectal balloon with effort. this group was divided on the basis of measurement of colonic diameter into those with megacolon and those with a normal sized colon. the latter group was further divided into those with normal transit and those with slow transit. the results were compared with those recorded ...	2011	3198990
imaging of the airways by bronchoscintigraphy for the study of mucociliary clearance.	a method for functional imaging of the large airways (bronchoscintigraphy) has been developed. it is based on the administration of aerosolised albumin labelled with technetium-99m using a special inhalation technique to produce central airway deposition. the method was evaluated as a measure of mucociliary clearance by recording the movement of radioactivity in the airways of 11 healthy, non-smoking subjects on two separate days. a series of bronchoscintigrams was acquired at five minute interv ...	2011	3194864
flow cytometry and cytology as response indicators to m-vac (methotrexate, vinblastine, doxorubicin and cisplatin).	we evaluated 23 patients treated with neoadjuvant methotrexate, vinblastine, doxorubicin and cisplatin for muscle invasive bladder carcinoma with serial bladder wash flow cytometry and serial urinary cytology. the sensitivities of bladder wash flow cytometry and cytology in detecting bladder cancer were 74 and 47 per cent, respectively, based on subsequent diagnosis from biopsy and cystectomy specimens. exclusion of those cases without surface carcinoma increased the sensitivities of bladder was ...	2011	3193503
[a sliding compressive screw for synthesis of the femur neck].		2011	3176799
the effect of trypanosoma vivax and trypanosoma congolense infections on the reaction time and semen characteristics in the zebu bull.		1988	3167554
[influence of bovine parathyroid hormone-(1-34) on the contraction of the rat vas deferens and the effect of calcium].		1985	3158160
volume overload hypertrophy elicited by cold and its effects on myocardial capillarity.	capillarity and fiber cross-sectional areas were measured in the hearts of guinea pigs exposed to cold early during growth. twelve male guinea pigs were kept at 5 +/- 1 degrees c for 4-18 weeks. hearts were perfusion fixed via the aorta with a 2.5% glutaraldehyde, 1% formaldehyde-buffered solution, blocks were cut from left (lv) and right (rv) ventricles, post-fixed in oso4, dehydrated and embedded in spurr medium. blocks were cut transversely to fiber orientation, 0.5 micron thick, stained with ...	1985	3156396
[open approach to the subclavian vein for permanent cardiac stimulation].	in 97.5% of 562 successive attempts, puncture of the subclavian vein to introduce permanent pacemaker electrodes was made easier and safer by the incisional technique described in this report. the very low incidence (0.35%) of traumatic pneumothorax and the absence, after a long follow-up, of any infection or exteriorization of the pacemaker chamber were the main advantages of this technical variant.	2011	3156361
effect of different modes of dialysis on serum erythropoietin levels in pediatric patients. a report of the southwest pediatric nephrology study group.	the relative importance of erythropoietin (ep) and inhibitors of erythropoiesis in the development of anemia in pediatric patients with end-stage renal disease (esrd) was assessed in 82 patients: 41 treated with peritoneal dialysis (pd) and 41 with hemodialysis (hd). serum ep was determined with a sensitive radioimmunoassay. potential serum inhibition of erythroid (cfu-e) and granulocytic (cfu-gm) progenitor cell growth was assessed using human bone marrow cell cultures. the mean ep level for al ...	1988	3153056
serum ferritin as the most sensitive measure of iron stores in pregnant women.		2011	3149686
production and purification of anthrax toxin.		1988	3148094
mineral density of bone in the forearm in premenopausal women with fractured wrists.		2011	3144371
acceleration of starvation- and glycerol-induced myxospore formation by prior heat shock in myxococcus xanthus.	the effect of heat shock on myxococcus xanthus was investigated during both glycerol- and starvation-induced development. cells heat shocked at 40 degrees c for 1 h prior to a development-inducing signal displayed an accelerated rate of myxospore formation at 30 degrees c. additionally, m. xanthus cells heat shocked prior to glycerol induction formed a greater total number of myxospores when sporulation was complete than did control cells maintained at 30 degrees c. however, in starvation-induce ...	1988	3141380
isolation and characterization of chondroitin 6-sulfate proteoglycans present in the extracellular matrix of rabbit bone marrow.	the authors' previous studies showed that the glycosaminoglycans present in rabbit bone marrow were composed of chondroitin 6-sulfate (79%) and hyaluronic acid (16%). immunohistochemically the chondroitin 6-sulfate was demonstrated to be in bone marrow matrix constructing hematopoietic microenvironment. in this study the authors isolated and characterized these glycosaminoglycans in their macromolecular form (i.e., proteoglycans). bone marrow of 3-month-old rabbits was defatted with organic solv ...	1988	3135865
ethanol and its novel metabolites inhibit insulin action on adipocytes.	the effects of ethanol, acetaldehyde, and the two novel metabolites of ethanol 2,3-butanediol and 1,2-propanediol on basal and insulin-stimulated adipocyte lipogenesis and glucose oxidation in vitro were investigated. whereas ethanol and acetaldehyde inhibited both processes at concentrations far greater than those found in alcoholic subjects, the two diols were extremely potent inhibitors of basal and insulin-stimulated adipocyte metabolism at concentrations far below those observed in alcoholi ...	1988	3133260
flumazenil (anexate) in severe intoxication with carbamazepine (tegretol).	in addition to the benzodiazepine antagonism we report, as a first observation, the effect of flumazenil on carbamazepine intoxication, where a mechanism other than benzodiazepine receptor antagonism must be supposed.	2011	3133214
clinical carpal scaphoid injuries.		2011	3133034
primary localized amyloidosis of the bladder with a monoclonal plasma cell infiltrate.	a case of primary tumour-like amyloidosis of the urinary bladder is described. the plasma cell infiltrate around the amyloid deposition revealed monoclonality by both immunoperoxidase and immunofluorescence methods and the amyloid gave a staining reaction consistent with al type. a local tissue based immunocyte dyscrasia appears to be responsible for the tumour-like amyloid deposition.	2011	3131723
protein fluorescence quenching by small molecules: protein penetration versus solvent exposure.	experiments were done to test the thesis that acrylamide and similar small molecules can penetrate into proteins on a nanosecond time scale. the approach taken was to measure the pattern of fluorescence quenching exhibited by quenching molecules differing in molecular character (size, polarity, charge) when these are directed against protein tryptophans that cover the whole range of tryptophan accessibility. if quenching involves protein penetration and internal quencher migration, one expects t ...	2011	3130621
arachidonic acid, prostaglandin e2 and f2 alpha inhibit leucine degradation in chick skeletal muscle.	arachidonic acid (5 microm), prostaglandin e2 (0.28 microm) and f2 alpha (14 microm) inhibited (p less than 0.01) the rates of net leucine transamination, leucine oxidative decarboxylation and total co2 production from leucine in extensor digitorum communis muscles from fed ten-day-old chicks. indomethacin (50 microm) markedly inhibited (p less than 0.01) the rate of pge2 production in the presence of 5 microm arachidonic acid and prevented the inhibition of leucine degradation by arachidonic ac ...	1988	3130060
characterization of a-i-containing lipoproteins in subjects with a-i milano variant.	the a-i milano variant of apolipoprotein a-i (a-im), by virtue of its arg-173----cys substitution, is capable of forming a disulfide bond with the 77-amino-acid apolipoprotein a-ii polypeptide (a-iis) as well as with itself to produce dimers, a-im/a-iis and a-im/a-im, respectively. a-i-containing lipoproteins (lp): particles with a-ii (lp(a-i with a-11)) and particles without a-ii (lp(a-i without a-ii)) in the plasma of two nonhyperlipidemic a-im carriers were investigated to determine the effec ...	1988	3129016
genentech's boom is boosted by new clinical trial data.		2011	3127729
specimen collection and handling.		2011	3127719
recovery of mitomycin c-treated mouse 10t1/2 cells during confluent holding.	the relationship between cytotoxicity, sister-chromatid exchanges (sce) and the repair of dna crosslinks was studied in mouse 10t1/2 cells during confluent holding following either acute or protracted mmc treatment. no cytotoxic effects were observed with increasing doses of mmc until sce frequencies 1.8 times background levels were induced. protracted mmc treatments were less cytotoxic than acute mmc exposure at doses which yielded similar frequencies of sce. the kinetics of recovery during con ...	1988	3127697
effects of the antimicrobic tiamulin on seven gram-negative bacterial fish pathogens.	in vitro and in vivo test were carried out with tiamulin and gram-negative bacterial pathogens of fish. determination of minimum inhibitory concentration for 51 strains of seven species of gram-negative bacterial pathogens showed that only strains of vibrio anguillarum were sensitive at 1.6-6.25 ppm, while the rest of test strains required 25- greater than 100 ppm. control of infection was not achieved when tiamulin was fed for 14 days at 5 or 50 mg/kg to rainbow trout (salmo gairdneri) experime ...	1988	3127604
regulation of intestinal apolipoprotein b synthesis and secretion by caco-2 cells. lack of fatty acid effects and control by intracellular calcium ion.	to investigate the mechanism of control of intestinal apolipoprotein b (apob) secretion, we studied the effects of fatty acids and calcium ionophores on the human intestinal model cell line caco-2. although treatment with various fatty acids (18:1w9, 18:2w6, and 20:5w3) complexed to bovine serum albumin resulted in a dramatic redistribution of apob-100 from the low density and high density lipoproteins to the very low density lipoprotein fraction, there was no effect of any of the fatty acids on ...	1988	3125180
ovarian stimulation for disordered ovulatory cycles.		2010	3109361
topical mupirocin in the treatment of bacterial skin infections.	mupirocin is an investigational topical antibiotic used for treatment and prophylaxis of bacterial skin infections. mupirocin differs from other antibiotics in its synthesis, structure, and mechanism of action. in vitro, mupirocin possesses antimicrobial activity against staphylococci, streptococci, hemophilus influenzae, and neisseria gonorrhoeae. few studies comparing mupirocin to other topical antibiotics are available. initial studies comparing mupirocin to inactive vehicle in the treatment ...	1986	3102197
topical mupirocin in the treatment of bacterial skin infections.	mupirocin is an investigational topical antibiotic used for treatment and prophylaxis of bacterial skin infections. mupirocin differs from other antibiotics in its synthesis, structure, and mechanism of action. in vitro, mupirocin possesses antimicrobial activity against staphylococci, streptococci, hemophilus influenzae, and neisseria gonorrhoeae. few studies comparing mupirocin to other topical antibiotics are available. initial studies comparing mupirocin to inactive vehicle in the treatment ...	1986	3102197
hemolytic disease of the newborn caused by anti-lan, anti-jka, and anti-c.		2011	3101246
repression and turnover pattern fushi tarazu rna in the early drosophila embryo.	embryonic expression of transcripts from the drosophila gene fushi tarazu (ftz) progresses through a series of spatial patterns, culminating in a seven-banded pattern at the cellular blastoderm stage. we studied the generation of this pattern using inhibitors of rna synthesis (alpha-amanitin) and protein synthesis (cycloheximide). injections of alpha-amanitin revealed that ftz rna turns over extremely rapidly in the embryo, and we think that this may be essential to effect rapid changes in ftz r ...	2011	3096577
serum diamine oxidase in the neonate.	serum diamine oxidase (dao) activities were measured in 9 pregnant women and in 33 neonates during the 1st week of life. the radiometric method was used. very high values were found in the mothers (mean +/- se: 720 +/- 143 pmol ml-1 h-1). neonates showed significantly elevated values (mean +/- se: 178 +/- 54 pmol ml-1 h-1) on the 1st postnatal day when compared to the 7th day values (mean +/- se: 27 +/- 5.7 pmol ml-1 h-1). serial determinations showed a progressive decline from day 1 to day 7 af ...	2011	3091095
[value of ultrasonography in the diagnosis of congenital hip dysplasia in children].		2010	3075749
[value of ultrasonography in the diagnosis of congenital hip dysplasia in children].		2010	3075749
cell volume regulation in renal cortical cells.	both proximal renal tubule cells and cultured madin-darby canine kidney (mdck) cells are capable of regulating their volume in hypotonic media. regulatory cell volume decrease in proximal straight tubules is impaired by barium, amiloride and acetazolamide and depends on the presence of bicarbonate and of sodium, whereas it is unaffected by complete removal of extracellular chloride. the observations may point to parallel loss of potassium through potassium channels as well as of bicarbonate and ...	2009	3074396
regulation of glucose storage in obesity and diabetes: metabolic aspects.		2011	3069399
physiology of alpine skiing.	physiological profiles of elite alpine skiers reveal the importance of muscular strength, anaerobic power, anaerobic endurance, aerobic endurance, coordination, agility, balance, and flexibility. on-hill snow training and dryland training programmes should focus on the elevation of these fitness components. physical characteristics of elite skiers reveal an average height and body mass. today, successful skiers are taller and heavier than their predecessors. slalom skiers tend to be leaner than ...	2011	3067309
[the activity of the control de vectores during the 50th anniversary year of the instituto de medicina tropical "pedro kourí"].		1988	3067282
[the activity of the control de vectores during the 50th anniversary year of the instituto de medicina tropical "pedro kourí"].		1988	3067282
[lithium, manic depressive psychoses and biological rhythms].		2011	3063381
[alcohol and endocrinologic homeostasis].	in addition to direct toxic effects on endocrine organs chronic alcohol intake affects regulation of endocrine systems by disturbed liver function. as a result in patients with alcohol-induced liver cirrhosis gonadal axis is characterized by low total and free testosterone, elevated estradiol. lh, fsh, and sexual hormone binding globulin and an enhanced conversion of testosterone to estradiol. prolactin also is found to be elevated. the thyrotropic axis is characterised by low t3- und t4- as wel ...	1988	3062942
the laboratory response to glucagon dosages used in gastrointestinal examinations.	laboratory studies occasionally are necessary for patients who have undergone hypotonic gastrointestinal examinations. to ascertain the effects of glucagon on these patients, we determined the biochemical and hematologic responses to doses of 0.25-2 mg of glucagon in a double-blind crossover study. when glucagon was given intravenously or intramuscularly in increasing doses, serum values for glucose and insulin increased linearly up to 1 mg with a slight decrease at 2 mg. after intravenous and i ...	2011	3061974
the laboratory response to glucagon dosages used in gastrointestinal examinations.	laboratory studies occasionally are necessary for patients who have undergone hypotonic gastrointestinal examinations. to ascertain the effects of glucagon on these patients, we determined the biochemical and hematologic responses to doses of 0.25-2 mg of glucagon in a double-blind crossover study. when glucagon was given intravenously or intramuscularly in increasing doses, serum values for glucose and insulin increased linearly up to 1 mg with a slight decrease at 2 mg. after intravenous and i ...	2011	3061974
reflex sympathetic dystrophy syndrome in children and adolescents. report of 18 cases and review of the literature.	reflex sympathetic dystrophy syndrome is a well-recognized disorder in adults, but it is rarely diagnosed in the pediatric age group. this report summarizes our experience with this condition from 1975 to 1985. we diagnosed, treated, and followed up this condition in 18 children and adolescents. the condition usually followed trauma. the most prominent feature in all patients was a constant limb pain with episodes of paroxysmal exacerbation. the pain was associated with two or more of the follow ...	2011	3057867
effects of peptide hi on basal and stimulated insulin and glucagon secretion in the mouse.	peptide hi (phi) is a peptide with 27 amino acids that is structurally similar to vip (vasoactive intestinal peptide). since phi, like vip, has been demonstrated to occur in intrapancreatic neurons, and since vip earlier was shown to stimulate insulin and glucagon secretion in the mouse, we investigated whether also phi affects islet hormone secretion in this species. phi was thereby injected intravenously at dose levels between 0.5 and 8.0 nmol/kg. it was found that phi did not affect basal lev ...	1988	3047591
[breast carcinomas and the extracellular matrix].	this paper underlines the interrelations between tumoral cells and the extra-cellular matrix in breast cancers with possible applications for the diagnosis and the prognosis. in mammary carcinomas, the first step of tumoral invasion is characterized by the loss of basement membrane components, particularly type iv collagen and laminin. immunohistochemical detection of these disruptions of basement membrane is easy and useful for the diagnosis of "in situ" or microinvasive carcinomas. laminin see ...	2010	3041983
regulation of fructose 2,6-bisphosphate levels in neurospora crassa.	both wild type and cr-1 mutant (adenylate cyclase and cyclic amp-deficient) strains of neurospora crassa contain fructose 2,6-bisphosphate at levels of 27 nmol/g dry tissue weight. this level decreases by about 50% in both strains upon depriving the cells of carbon or nitrogen sources for 3 h. an increase in cyclic amp levels produced by addition of lysine to nitrogen-starved cells produced no increase in fructose 2,6-bisphosphate levels. both strains respond to short-term addition of salicylate ...	2008	3040112
influence of dna length on spermine-induced condensation. importance of the bending and stiffening of dna.	using dna restriction fragments of 258 to 4362 base-pairs, we have investigated the influence of the dna length on the condensation process induced by spermine, with the aid of electric dichroism measurements. the 258- and 436 bp fragments condensed into rod-like particles, while the fragments of 748 bp or more condensed into torus-shaped particles. our results suggest that a dna molecule longer than the circumference of the toroids observed previously (680 bp) is required to serve as a nucleus ...	2008	3040099
vectors for expression and amplification of cdna in mammalian cells: expression of rat phenylalanine hydroxylase.	we have constructed two recombinant plasmid vectors for direct expression and amplification of cdna in mammalian cells. each vector carries two dominant selectable markers (the bacterial neo gene and the mouse dhfr gene), a promoter sequence (viral ltr in pav009/a+, and sheep metallothionein promoter in pmt010/a+), a polyadenylation signal sequence, and a bam hi site to allow insertion of cdna. we have used these vectors to prepare recombinant clones for the expression of rat phenylalanine hydro ...	1986	3028740
vectors for expression and amplification of cdna in mammalian cells: expression of rat phenylalanine hydroxylase.	we have constructed two recombinant plasmid vectors for direct expression and amplification of cdna in mammalian cells. each vector carries two dominant selectable markers (the bacterial neo gene and the mouse dhfr gene), a promoter sequence (viral ltr in pav009/a+, and sheep metallothionein promoter in pmt010/a+), a polyadenylation signal sequence, and a bam hi site to allow insertion of cdna. we have used these vectors to prepare recombinant clones for the expression of rat phenylalanine hydro ...	1986	3028740
sequence of the genome ends and of the junction between the ends in concatemeric dna of pseudorabies virus.	the nucleotide sequences of the termini of the mature pseudorabies virus genome and of the junction between these termini in concatemeric dna were compared. to ensure conservation of unmodified 5' and 3' termini, the end fragments obtained directly (uncloned) from mature viral dna were sequenced. the sequence obtained from 5' and 3' end labeling revealed that whereas the l terminus was blunt ended, the s terminus had a 2-base (gg) 3' overhang. the sequences spanning the junction between the term ...	1986	3023670
encephalomyocarditis virus 3c protease: efficient cell-free expression from clones which link viral 5' noncoding sequences to the p3 region.	all picornaviral peptides are derived by progressive posttranslational cleavage of a giant precursor polyprotein. translation of encephalomyocarditis virus (emc) rna in rabbit reticulocyte extracts produces active viral peptides, including protease 3c, which is responsible for many cleavage reactions within the processing cascade. dna plasmids containing 5' noncoding sequences of emc linked to other portions of the viral genome were constructed and transcribed into rna. like virion rna, the clon ...	1986	3021972
pain, increasing girth in healthy japanese man.		1986	3021791
pain, increasing girth in healthy japanese man.		1986	3021791
inhibition of erythrocyte ca2+-atpase by activated oxygen through thiol- and lipid-dependent mechanisms.	we have studied erythrocyte ca2+-atpase as a model target for elucidating effects of activated oxygen on the erythrocyte membrane. either intracellular or extracellular generation of activated oxygen causes parallel decrements in ca2+-atpase activity and cytoplasmic gsh, oxidation of membrane protein thiols, and lipid peroxidation. subsequent incubation with either dithiothreitol or glucose allows only partial recovery of ca2+-atpase, indicating both reversible and irreversible components which ...	2010	3021221
inhibition of erythrocyte ca2+-atpase by activated oxygen through thiol- and lipid-dependent mechanisms.	we have studied erythrocyte ca2+-atpase as a model target for elucidating effects of activated oxygen on the erythrocyte membrane. either intracellular or extracellular generation of activated oxygen causes parallel decrements in ca2+-atpase activity and cytoplasmic gsh, oxidation of membrane protein thiols, and lipid peroxidation. subsequent incubation with either dithiothreitol or glucose allows only partial recovery of ca2+-atpase, indicating both reversible and irreversible components which ...	2010	3021221
[pathology of the craniocervical junction as shown by magnetic resonance tomography. 68 cases].	in cases of a suspected lesion at the level of the cranio-cervical junction, mr should be considered as the imaging technique of choice. tumours, inflammation and degenerative processes can be reliably demonstrated and diagnosed. administration of contrast media, for example, gadolinium-dtpa, is necessary in selected cases only. comparing "examination time" of x-ray myelography and high resolution x-ray ct with mr, one can well tolerate the long "acquisition" of mr images.	2010	3020636
peptidoglycan synthetic activities in membranes of escherichia coli caused by overproduction of penicillin-binding protein 2 and roda protein.	penicillin-binding protein (pbp)-2 and the roda protein are known to function in determining the rod shape of escherichia coli cells. peptidoglycan biosynthetic reactions that required these two proteins were demonstrated in the membrane fraction prepared from an e. coli strain that overproduced both of these two proteins and which lacked pbp-1b activity (the major peptidoglycan synthetase activity in the normal e. coli membranes). the cross-linked peptidoglycan was synthesized from udp-n-acetyl ...	1986	3009484
the epidemiology of the acquired immunodeficiency syndrome.		1986	3008169
polyphosphoinositide metabolism in aging human erythrocytes.	normal human erythrocytes were fractionated in a density gradient. capacity to metabolize polyphosphoinositides was compared in young (least dense) and old (most dense) cells. polyphosphoinositide synthesis was assessed by following the incorporation of radioactivity from [gamma-32p]atp into the 1-(3-sn-phosphatidyl)-d-myo-inositol 4-phosphate (ptdins4p) and 1-(3-sn-phosphatidyl)-d-myo-inositol 4,5-bisphosphate (ptdins(4,5)p2) of isolated membranes. there was no significant age-dependent change ...	1985	3000548
an anonymous single copy x-chromosome clone dxs91, from xq11-q13, identifies a moderately frequent rflp.		2010	2997751
[membrane lipids and membrane enzymes].		2010	2993699
stimulation of a cell-mediated cytotoxic response to felv-induced t cell lymphomas in the cat.	nonspecific cell-mediated cytotoxicity was examined in the peripheral blood and spleens of normal and vaccinia virus-infected adult domestic cats. natural cytotoxic (nc)-like cells, as measured by lysis of vaccinia- or hsv-infected, adherent cat tongue cells, were found in both the spleen and peripheral blood of normal, nonimmune cats. cytotoxicity was expressed in a 16-hr assay but not in a 4-hr assay. natural killer (nk)-like cells, as measured by lysis of an felv-induced lymphoid tumor cell l ...	1985	2993416
[mechanism of ischemic disorders of fatty acid oxidation in heart mitochondria].	the article deals with the effect of permanent, temporal and total myocardial ischemia on the oxidation of acetate, hexanoate, palmitoylcarnitine and palmitoyl-coa in isolated rabbit heart mitochondria. all the three models of ischemia in different experimental situations demonstrated a similar degree of fatty acid oxidation suppression independent of the length of acyl residue, the suppression being the greatest during the first hours and in total ischemia. both in the control and in ischemia, ...	1985	2988585
[angiographic and surgical control of the vertebral and carotid pedicles supplying giant paraganglioma of the skull base and neck].	results obtained after excision of large jugular paragangliomas are largely conditioned by the possibility of control of the different vascular pedicles, and existing possibilities are discussed in relation to 6 briefly presented cases. the internal carotid artery can either be occluded radiologically by balloon catheter and then embolized if the artery is surrounded by a tumor, or controlled surgically if the artery is in contact with the tumor only and provides only a small proportion of its v ...	2010	2988396
[current approaches to immunological diagnosis of autoimmune and immune complex diseases].		2010	2974642
a study of the correlation between cyclophilin binding and in vitro immunosuppressive activity of cyclosporine a and analogues.	in order to establish whether cyp is the pharmacologically relevant csa receptor, the cyp binding v immunosuppressive activity was measured for an extensive, structurally varied group of csa analogues. overall, cyp binding was found to parallel immunosuppressive activity. other than meala6-csa, the few exceptions to the correlation could be ascribed to cellular metabolism. these results strongly implicate cyp or a related protein in the mechanism of action of cyclosporine.	2010	2966482
stimulation of growth hormone secretion by central administration of atrial natriuretic polypeptide in the rat.	intracerebroventricular (icv) injection of alpha-human atrial natriuretic polypeptide (alpha hanp) or alpha-rat anp (0.6 and 3 nmol/rat) elicited an increase in plasma gh levels both in conscious freely moving rats and in urethane-anesthetized rats when given at the trough of spontaneous gh secretion. antiserum specific for rat grf did not affect the plasma gh increase induced by icv injection of alpha hanp. intracerebroventricular injection of alpha anp (3 nmol/rat) failed to stimulate gh secre ...	1988	2966063
human milk-derived lactoferrin inhibits mitogen and alloantigen induced human lymphocyte proliferation.	purified lactoferrin (lf), isolated from human milk, was tested for its effect on human t-lymphocyte proliferative responses to phytohemagglutinin (pha) and to alloantigens in mixed lymphocyte culture. lf inhibited proliferation in both assays in a dose-dependent manner. the suppressive effect was not due to lf mediated cytotoxicity since washing cells that had been pre-incubated with lf restored their proliferative activity. lf was most effective in suppressing the pha response when added withi ...	1987	2963123
individually disparate anti-inflammatory in-vivo susceptibility to corticosteroids in renal transplant recipients.	we used the strength of suppression of the interleukin-1-induced peak crp response (crpmax) post-transplant as a measure for the individual steroid susceptibility in vivo. this suppressive effect proved to be fully reversible after abrogation of steroid therapy following graft removal. the standardized suppressive therapy immediately post-transplant led to extremely different results in individual patients ranging from no suppression (26.1% of the patients) to a strong or practically absolute su ...	2010	2959125
comparison of human growth hormone levels in children with satisfactory and unsatisfactory growth.	sixty-six children with satisfactory growth and 73 with unsatisfactory growth (both sexes) from three different age groups (1 year, 5 years and 10 years) were included in this study. it was observed that the serum concentrations of human growth hormone (hgh) in children with satisfactory growth ranged from 1.00 to 4.00 ng/ml and never exceeded 4.00 ng/ml. in a comparative study there was a significant increase in serum hgh levels in subjects of all age groups who were not growing satisfactorily. ...	1987	2956220