Publications

purification and identification of the factor capable of converting ca2+-atpase into mg2+-atpase present in rhodospirillum rubrum chromatophores.		1980	6244266
energy-linked reactions catalyzed by the purified atpase complex (f0f1) from rhodospirillum rubrum chromatophores.	1. the isolation of f0f1-atpase complex from rhodospirillum rubrum chromatophores by the use of taurodeoxycholate is described. 2. the enzyme preparation contains about 12 polypeptides; five are subunits of the f1 moiety. 3. the atpase activity of the purified enzyme is dependent on the addition of phospholipids. 4. km-vales for mg2+-atp and ca2+-atp are similar to the values obtained for the membrane-bound enzyme. 5. the f0f1-atpase complex is more than 70% inhibited by oligomycin and n,n'-dicy ...	1980	6447594
conversion of the ca2+-atpase from rhodospirillum rubrum into a mg2+-dependent enzyme by 1,n6-etheno atp.		1980	6448051
polyadenylated messenger rnas code for photo reaction center and light-harvesting antenna polypeptides of rhodospirillum rubrum.		1980	6766405
short-lived delayed luminescence of photosynthesizing organisms. ii. the ratio between delayed and prompt fluorescence as studied by the modulation method.	the ratio between the intensities of delayed and prompt fluorescence was studied for different photosynthetic objects under different conditions by modulation method. the method is based on excitation of luminescing objects by light, modulated harmonically, and on a combined study of phase shifts and demodulation coefficients of the luminescence as related to excitation light. the presence of intense delayed emissions was revealed in purple bacteria, ectothiorhodospira shaposhinokovii, rhodospir ...	1980	6768385
photo-induced electron transport and water state in rhodospirillum rubrum chromatophores.	it is shown that in bacterial chromatophores the pronounced changes in the free water content with a proton spin-spin relaxation time (t2) of 10(-3)--10(-2) s does not influence the efficiency of electron transfer from the photosynthetic reaction centre to the membrane pool of secondary acceptors. an abrupt inhibition of this process occurs only after the loss of the water with faster proton spin-spin relaxation time (t2 of 10(-4) s). the process is reversible. the water fraction in question is ...	1980	6768386
formation of intracytoplasmic membranes in chemotrophic mecillinam sphaeroplasts of rhodospirillum rubrum.	chemotrophically growing cells of rhodospirillum rubrum were transformed into sphaeroplasts of irregular shape under the influence of the penicillin antibiotic mecillinam. on a cell protein basis, respiratory activities of whole sphaeroplasts were slightly decreased as compared with the untreated control. but, on a membrane protein basis various activities of the respiratory chain were largely identical in membrane preparations from untreated cells and sphaeroplasts, respectively. chemotrophical ...	1980	6769673
activationless electron transfer processes in biological systems.		1980	6766403
purification and properties of polynucleotide phosphorylase from photosynthetic bacterium rhodospirillum rubrum.	1. polynucleotide phosphorylase [polyribonucleotide: orthophosphate nucleotidyltransferase, ec 2.7.7.8] was purified to near homogeneity from the photosynthetic bacterium, rhodospirillum rubrum. the purified enzyme had a molecular weight of approximately 160,000, and consisted of two equivalent subunits of approximately 76,000 daltons. it catalyzed the three reactions described below. 2. in the exchange reaction of the beta-phosphate of nucleoside diphosphates with pi by the purified enzyme in t ...	1980	6766923
detection of cytochrome b+50 in membranes of rhodospirillum rubrum isolated from aerobically and phototrophically grown cells.	1. dark equilibrium potentiometric titrations were conducted on membranes purified from rhodospirillum rubrum in an effort to identify b-type cytochrome components reported in other rhodospirillaceae. in preparations from aerobically grown cells virtually devoid of bacteriochlorophyll a, three components were observed at 560-540 nm. their oxidation-reduction midpoint potentials assigned by computer-assisted analysis were +195, +50 and -110 mv at ph 7.0; each of these fitted closely to theoretica ...	1980	6769433
[pyrophosphate-dependent d-fructose-6-phosphate-phosphotransferase in rhodospirillaceae (author's transl)].	a pyrophosphate-dependent fructose-6-phosphate phosphotransferase from the photosynthetic bacterium rhodospirillum rubrum was partially purified and characterized in respect to kinetic and regulatory properties. the enzyme had a molecular weight of about 95 000 dalton and required mg2+-ions for catalysis and maintenance of activity. the phosphotransferase was specific for fructose-6-phosphate (f-6-p) and inorganic pyrophosphate (pp) as substrates of the fructose-1,6-bisphosphate-forming reaction ...	1980	6446207
differences in the architecture of cytoplasmic and intracytoplasmic membranes of three chemotrophically and phototrophically grown species of the rhodospirillaceae.	freeze-fracture faces of membranes of either chemotrophically or phototrophically grown rhodospirillum rubrum, rhodopseudomonas sphaeroides, and rhodospirillum tenue were analyzed. all three species differed from each other with respect to size as well as numerical density (number per square micrometer) of intramembrane particles. in r. rubrum the number of particles on exoplasmic fracture faces of the cytoplasmic membrane stayed nearly constant (about 900 particles per microns2), but on the pla ...	1980	6776096
atp synthesis catalyzed by the atpase complex from rhodospirillum rubrum reconstituted into phospholipid vesicles together with bacteriorhodopsin.		1980	6451197
[thiosulfate oxidation by nonsulfur purple bacteria].	the capability to oxidize thiosulfate was studied in 11 cultures of purple bacteria belonging to rhodomicrobium vannielii, rhodopseudmonas viridis, rh. sphaeroides, rh. capsulata, and rhodospirillum rubrum. all the bacteria oxidized thiosulfate under aerobic conditions in the dark. the strains 2r, 8259, a1, a2 and d1 of rh. sphaeroides oxidized thiosulfate under anaerobic conditions in the light, and the process was coupled with carbon dioxide fixation. all the strains contained thiosulfate redu ...	1980	6777642
[conformational mobility and theory of the mössbauer effect in biological systems. (model of a brownian oscillator strongly damped for conformational modes)].	specific features of the mössbauer effect on proteins are discussed. it is found that the temperature dependence of recoilless gammaquantum absorption, f'(t), cannot be explained in terms of solid effects or diffusion spectrum broadening in liquids. a sharp decrease in f'(t) at temperature above 200 degrees k that has been found is due to the specificity of the conformational mobility of proteins and due to a smaller correlation time for spacially-confined diffusion of a mössbauer label tracer b ...	1980	6777658
effect of l-methionine-dl-sulphoximine on the photoproduction of hydrogen by rhodospirillum rubrum.	the repression of photoproduction of hydrogen by ammonia could be relieved by l-methionine-dl-sulfoximine. in the absence of ammonia, hydrogen evolution was inhibited by concentrations of l-methionine-dl-sulfoximine higher than 0.1 mm.	1980	6774882
the dependency of proton extrusion in the light on the developmental stage of the photosynthetic apparatus in rhodospirillum rubrum.	the rate of proton extrusion by whole cells of rhodospirillum rubrum is constant on a bacteriochlorophyll basis only above cellular bacteriochlorophyll concentrations of about 10 nmol bacteriochlorophyll per mg cell protein. at specific bacteriochlorophyll cellular levels below this value, the rate of proton extrusion per bacteriochlorophyll increases. correspondingly, membrane preparations isolated from these cells exhibit increases in the rate of proton uptake on a pigment basis. concomitant w ...	1980	6770898
energy transfer and bacteriochlorophyll fluorescence in purple bacteria at low temperature.	emission spectra of bacteriochlorophyll a fluorescence and absorption spectra of various purple bacteria were measured at temperatures between 295 and 4 k. for rhodospirillum rubrum the relative yield of photochemistry was measured in the same temperature region. in agreement with earlier results, sharpening and shifts of absorption bands were observed upon cooling to 77 k. below 77 k further sharpening occurred. in all species an absorption band was observed at 751-757 nm. the position of this ...	1980	6772218
[linear dichroism of pigments associated with spherical chromatophores. models of orientation in polyacrylamide gels].	linear dichroism and orientation of pigments in chromatophores of photosynthetic bacteria chromatium minutissimum and rhodospirillum rubrum using a novel method of orientation in polyacrylamide gel was studied. a model is proposed for orientation of spherical membranes of chromatophores or other similar vesicules. the value of linear dichroism is derived for known deformation of the gel and a certain angle between the transition dipole and a unit vector perpendicular to the membrane plane. the a ...	1980	6772937
[photoinduced changes in dielectric losses in the microwave region in chromatophores of rhodospirillum rubrum photosynthesizing bacteria].		1980	6772240
[photoinduced changes in dielectric losses in the microwave region in rhodospirillum rubrum photosynthesizing bacteria].		1980	6772239
polarographic measurement of h2 in aqueous solutions.		1980	6776845
[cyclic electron transfer and membrane potential generation in chromatophores on non-sulfur bacteria rhodospirillum rubrum].	the uptake of permeant anions by cells and chromatophores of the non-sulfur purple bacteria r. rubrum has been studied. antimycin a causes biphasic inhibition of the light-induced uptake of tetraphenylborate anions (tb-) by the cells and the isolated chromatophores incubated under anaerobic conditions. the first phase is observed at small concentrations of antimycin and is due to its effect as an inhibitor of the cyclic electron transfer. the second phase is observed at concentrations higher tha ...	1980	6783130
carotenoid triplet yields in normal and deuterated rhodospirillum rubrum.	quantum yields of carotenoid triplet formation in rhodospirillum rubrum wild type and fully deuterated cells and chromatophores were determined in weak laser flashes for excitation wavelength lambda i = 530 nm (mainly absorbed by the carotenoid spirilloxanthin) and for lambda i = 608 nm (mainly absorbed by bacteriochlorophyll) in the presence and absence of magnetic fields. all experiments were performed at room temperature and in the absence of oxygen. the quantum yield of reaction center bacte ...	1980	6773564
evidence for a correlation between the photoinduced electron transfer and dynamic properties of the chromatophore membranes from rhodospirillum rubrum.		1980	6773810
the photoreaction center of rhodospirillum rubrum mutant strain f24.1.	rhodospirillum rubrum strain f24.1 is a spontaneous revertant of nonphototrophic mutant f24 derived from wild-type strain s1. strain f24 shows no detectable photochemical activity and contains, at most, traces of the photoreaction center polypeptides. strain f24.1 has a phototrophic growth rate close to that of the wild-type strain (picorel, r., del valle-tascón, s. and ramírez, j.m. (1977) arch. biophys. biochem. 181, 665-670) but shows little photochemical activity. light-induced absorbance ch ...	1980	6775699
primary structure of a high potential, four-iron-sulfur ferredoxin from the photosynthetic bacterium rhodospirillum tenue.	the amino acid sequence of a high oxidation-reduction potential iron-sulfur protein (hipip) isolated from the purple photosynthetic bacterium rhodospirillum tenue has been determined. this is the smallest of the hipip's, containing 63 residues, with only 3 residues apparently conserved in addition to the 4 cluster-binding cysteines. a minimum of four internal genetic gaps is postulated to align tenue high potential iron-sulfur protein with the previously known proteins. it is the most divergent ...	1979	762147
characterization of the membrane-bound inorganic pyrophosphatase in rhodospirillum rubrum.	the membrane-bound inorganic pyrophosphatase (ec 3.6.1.1) from rhodospirillum rubrum has been investigated with the tools of enzyme kinetics, and with two amino acid reagents, n-ethyl-maleimide (malnet) and 4-chloro-7-nitrobenzofurazan (nbf-cl). 1. the concentration of the true substrate, mgppi, was varied with constant concentrations of free mg2+ or ppi. it was observed that mg2+ acted as an activator. 2. heat inactivation of the enzyme at 62 degrees c was slowed down in the presence of mg2+. 3 ...	1979	230038
regulation of cyclic photophosphorylation in rhodospirillum rubrum by the redox state of nicotinamide-adenine dinucleotide.	we have investigated the effect of the redox state of added nad on the rates of anaerobic cyclic photophosphorylation which are supported by membrane vesicles isolated from rhodospirillum rubrum. as the redox potential of nad was lowered, the activity decreased according to a typical potentiometric titration. the nernst plot showed an apparent midpoint potential (e'o) of -350 mv and had a slope which corresponded to a two-electron transition. besides, an almost identical potentiometric relations ...	1979	223636
iron-sulfur clusters in the molybdenum-iron protein component of nitrogenase. electron paramagnetic resonance of the carbon monoxide inhibited state.	carbon monoxide inhibits reduction of dinitrogen (n2) by purified nitrogenase from azotobacter vinelandii and clostridium pasteurianum in a noncompetitive manner (kii and kis = 1.4 x 10(-4) and 4.5 x 10(-4) and 7 x 10(-4) atm and 14 x 10(-4) atm for the two enzymes, respectively). the onset of inhibition is within the turnover time of the enzyme, and co does not affect the electron flux to the h2-evolving site. the kinetics of co inhibition of n2 reduction are simple, but co inhibition of acetyl ...	1979	228701
orientation of the bacteriochlorophyll triplet and the primary ubiquinone acceptor of rhodospirillum rubrum in membrane multilayers determined by esr spectroscopy (i).	chromatophores from rhodospirillum rubrum were oriented as multilayers on quartz slides under reducing conditions. irradiation of these multilayers in the resonance cavity of an esr spectrometer at 6 k yielded the spectrum of the bacteriochlorophyll dimer triplet. the relative intesities of the main six lines of the triplet were dependent on the angle subtended by the direction of the external magnetic field with plane of the multilayers. the angular dependence of the intensities of these transi ...	1979	228709
properties of the solubilized membrane-bound hydrogenase from the photosynthetic bacterium rhodospirillum rubrum.		1979	224815
[free radical centers in the chromatophores and preparations of rhodospirillum rubrum reaction centers].	the light-induced free radical esr signals in chromatophores and reaction center preparation of r. rubrum were studied. it has been shown that the properties of the signals in both systems were strictly identical. at the microwave powers higher than 10 mw the esr signal at g approximatley 2.00 is saturated and its asymmetry arises. changes of the esr signal shape may be explained by the superposition of p870+ signal with that from the ubisemiquinone. addition of ferricyanide to chromatophores in ...	1979	224953
comparison of glyoxalase i purified from yeast (saccharomyces cerevisiae) with the enzyme from mammalian sources.	glyoxalase i from yeast (saccharomyces cerevisiae) purified by affinity chromatography on s-hexylglutathione-sepharose 6b was characterized and compared with the enzyme from rat liver, pig erythrocytes and human erythrocytes. the molecular weight of glyoxalase i from yeast was, like the enzyme from rhodospirillum rubrum and escherichia coli, significantly less (approx. 32000) than that of the enzyme from mammals (approx. 46000). the yeast enzyme is a monomer, whereas the mammalian enzymes are co ...	1979	393249
activation of ribulose 1,5-bisphosphate carboxylase from rhodopseudomonas sphaeroides: probable role of the small subunit.	the activation properties of the form i and form ii ribulose 1,5-bisphosphate carboxylases from rhodopseudomonas sphaeroides were examined. both enzymes have a requirement of mg2+ for optimal activity. mn2+, ni2+, and co2+ can also support activity of the form i enzyme, whereas only mn2+ can substitute for mg2+ with the form ii enzyme. the effect of different preincubations on the carboxylase reaction was also examined. both enzymes exhibited a lag when preincubated with other than mg2+ and co2 ...	1979	316430
the amino acid sequence of cytochrome c' from the purple sulphur bacterium chromatium vinosum.	an amino acid sequence is proposed for the cytochrome c' from the photosynthetic purple sulphur bacterium chromatium vinosum strain d. it is single polypeptide chain of 131 residues, with haem-attachment cysteine residues at positions 121 and 124. the results discredit an earlier report [dus, bartsch & kamen (1962) j. biol. chem 237, 3083--3093] of a di-haem peptide sequence from this protein. the sequence belongs to the same class as the published alcaligenes and rhodospirillum rubrum cytochrom ...	1979	220951
[determination of the distance between charges following their photoseparation in chromatophores from r. rubrum].		1979	221054
anomalies in amino acid sequences of small cytochromes c and cytochromes c' from two species of purple photosynthetic bacteria.		1979	221823
purification of the energy-transducing adenosine triphosphatase complex from rhodospirillum rubrum.	the oligomycin- and n,n'-dicyclohexylcarbodiimide-sensitive adenosine triphosphatase complex extracted with triton x-100 from the chromatophores of rhodospirillum rubrum was extensively purified. the purification procedure included (diethylamino)ethylcellulose chromatography and glycerol gradient centrifugation. the specific activity of mg2+-dependent atp hydrolysis in the purified preparation increased about 11-fold, while that of ca2+-dependent atp hydrolysis increased 50-fold as compared with ...	1979	157774
[effect of formate on the respiration of different microorganisms].	the cells of methylotrophic bacteria (achromobacter parvulus 1, pseudomonas methylica 2 and 20, ps. fluorescens 45, ps. oleovorans 52) and candida methylica 101 grown in a medium with methanol take up oxygen in the presence of formiate. a. parvulus is most resistant to formiate (km = 2.3 +/- 0.5) x 10(-3) m; ki = 4 x 10(-1) m). when grown on cn substrates, these microorganisms cannot oxidize formiate (with an exception of a. parvulus). formiate also serves as a respiration substrate for e. coli ...	1979	481274
the chronoamperometric determination of homogeneous small molecule-redox protein reaction rates.		1979	224724
[possible pathways for acetyl-coa formation by purple bacteria].	purple sulfur (ectothiorhodospira shaposhnikovii, chromatium minutissimum, lamprobacter modestohalophilus, thiocapsa roseopersicina) and nonsulfur (rhodospirillum rubrum, rhodopseudomonas palustris, rhodopseudomonas spheroides) bacteria are capable of forming acetyl-coa synthetase, phosphotransacetylase and acetokinase independent of the medium composition and growth conditions. in all of the purple sulfur bacteria with an exception of e. shaposhnikovii, the activity of acetokinase is much highe ...	1979	228168
transfer of light-induced electron-spin polarization from the intermediary acceptor to the prereduced primary acceptor in the reaction center of photosynthetic bacteria.	in reaction centers and chromatophores of photosynthetic bacteria strong light-induced emissive esr signals have been found, not only after a flash but also under continuous illumination. the signal, with g = 2.0048 and delta hpp = 7.6 g, is only present under reducing conditions in material in which the primary acceptor, ubiquinone, u and its associated high-spin ferrous ion are magnetically uncoupled. its amplitude under continuous illumination is strongly dependent on light intensity and on m ...	1979	228714
[reconstitution of electrogenic function of pyrophosphatase isolated from rhodospirillum rubrum membranes].	the membrane vesicles (proteoliposomes) have been reconstituted from phospholipids and inorganic pyrophosphatase (ec 3.6.1.1) isolated from rhodospirillum rubrum chromatophores. an addition of inorganic pyrophosphate (ppi) causes a mg2+-dependent formation of a transmembrane electric potential difference and an uptake of penetrating tetraphenylborate anions by the proteoliposomes. thus, isolated pyrophosphatase, being incorporated into the phospholipid membrane, functions as a mgppi-dependent pr ...	1979	232668
isolation, characterization, and crystallization of ribulosebisphosphate carboxylase from autotrophically grown rhodospirillum rubrum.	serial culture of rhodospirillum rubrum with 2% co2 in h2 as the exclusive carbon source resulted in a rather large fraction of the soluble protein (greater than 40%) being comprised of ribulosebisphosphate carboxylase (about sixfold higher than the highest value previously reported). isolation of the enzyme from these cells revealed that it has physical and kinetic properties similar to those previously described for the enzyme derived from cells grown on butyrate. notably, the small subunit (w ...	1979	33152
proton nuclear magnetic resonance studies of rhodospirillum rubrum cytochrome.	rhodospirillum rubrum cytochrome c2 was studied by proton nuclear magnetic resonance at 220 mhz. assignments were made to the resonances of heme c by double-resonance techniques and by temperature-dependence studies. the aromatic resonances of trp-62 and tyr-70 of ferrocytochrome c2 were identified by spin-decoupling experiments. the resonances of the met-91 methyl group of the ferri- and ferrocytochromes were assigned by saturation-transfer experiments. the assignments are compared to those mad ...	1979	34433
[activation and inhibition of photoinduced proton absorption in rhodospirillum rubrum chromatophores by detergents and solvents].	the effects of detergents (triton x-100) and solvents (diethyl ether, metanol) on the reversible light-induced proton uptake, photophosphorylation and band shift of the carotenoid in chromatophores from r. rubrum are described. all these compounds were found to stimulate the extent of light-induced proton uptake with subsequent inhibition when the concentrations were increased. stimulation of proton uptake is accompanied by inhibition of both phosphorylation and carotenoid absorbance shift.	1979	37926
blue and red shifts of bacteriochlorophyll absorption band around 880 nm in rhodospirillum rubrum.	the redox potential dependence of the light-induced absorption changes of bacteriochlorophyll in chromatophores and subchromatophore pigment-protein complexes from rhodospirillum rubrum has been examined. the highest values of the absorption changes due to the bleaching of p-870 and the blue shift of p-800 in chromatophores and subchromatophore complexes are observed in the 360-410mv redox potential range. at potentials below 300 mv (ph 7.0), the 880 nm band of bacteriochlorophyll shifts to shor ...	1979	41575
the kinetics of photooxidation of c-type cytochromes by rhodospirillum rubrum reaction centers.		1979	41489
manganese, an essential trace element for n2 fixation by rhodospirillum rubrum and rhodopseudomonas capsulata: role in nitrogenase regulation.	nitrogenase (n(2)ase) from the photosynthetic bacterium rhodospirillum rubrum can exist in two forms, an unregulated form (n(2)ase a) and a regulatory form (n(2)ase r), the latter being identified in vitro by its need for activation by a mn(2+)-dependent n(2)ase activating system. the physiological significance of this mn(2+)-dependent n(2)ase activating system was suggested here by observations that growth of r. rubrum and rhodopseudomonas capsulata on n(2) gas (a condition that produces active ...	1979	42641
[shifts of the bacteriochlorophyll absorption band at 880 nm in chromatophores and subchromatophore pigment-protein complexes from rhodospirillum rubrum].	the redox potential dependency of the light-induced absorption changes of bacteriochlorophyll in the chromatophores and subchromatophore particles from rhodospirillum rubrum has been studied. the highest values of the absorption changes due to the bleaching of p870 and the blue shift of p800 are observed within the redox potential range of 360--410. at the potential values below 300 mv the 880 nm band of bacteriochlorophyll shifts to shorter wavelengths in the subchromatophore particles and to l ...	1979	41599
oxonol dyes as monitors of membrane potential. their behavior in photosynthetic bacteria.	the reponses of oxonol dyes to single and multiple single turnovers of the photosynthetic apparatus of photosynthetic bacteria have been studied, and compared with the responses of the endogenous carotenoid pigments. the absorbance changes of the oxonols can be conveniently measured at 587 nm, because this is an isosbestic point in the 'light-minus-dark' difference spectrum of the chromatophores. the oxonols appear to respond to the light-induced 'energization' by shifting their absorption maxim ...	1979	103582
resolution and reconstitution of rhodospirillum rubrum pyridine dinucleotide transhydrogenase: chemical modification with n-ethylmaleimide and 2,4-pentanedione.		1979	39595
two forms of nitrogenase from the photosynthetic bacterium rhodospirillum rubrum.	acetylene reduction by nitrogenase from rhodospirillum rubrum, unlike that by other nitrogenases, was recently found by other investigators to require an activation of the iron protein of nitrogenase by an activating system comprising a chromatophore membrane component, adenosine 5'-triphosphate (atp), and divalent metal ions. in an extension of this work, we observed that the same activating system was also required for nitrogenase-linked h(2) evolution. however, we found that, depending on the ...	1979	106042
characterization of a succinate dehydrogenase complex solubilized from the cytoplasmic membrane of bacillus subtilis with the nonionic detergent triton x-100.	a succinic dehydrogenase (sdh) complex has been purified from triton x-100-solubilized membranes from bacillus subtilis by precipitation with specific antibody. radioactively labeled precipitated complex was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography of the gels. the complex contained equimolar amounts of three polypeptides with approximate molecular weights of 65,000, 28,000, and 19,000. five succinic dehydrogenase-negative mutants, belongi ...	1979	108258
photooxidase activity of rhodospirillum rubrum chromatophores and reaction center complexes. the role of non-cyclic electron transfer in generation of the membrane potential.		1979	109117
characterisation of reaction centers and their phospholipids from rhodospirillum rubrum.	1. reaction centers from rhodospirillum rubrum have been extracted with the zwitterionic detergent lauryl dimethyl amine oxide. subsequent purification has been achieved by gel filtration and ion-exchange chromatography. the pure reaction centers are composed of three protein subunits (l, m, h), bacteriocholorophyll and bacteriopheophytin in the ratio 2 : 1 and phospholipids. 2. the phospholipid composition has been found to be similar to that of whole chromatophore membrane, except that diphosp ...	1979	109118
activation of ala synthetase by reduced thioredoxin in rhodopseudomonas spheroides y.		1979	109312
membrane potential and reconstitution.		1979	110998
[conformational regulation of functional activity of photosynthetic membranes of purple bacteria].	in reaction centres of photosynthetic membranes of r, rubrum the efficiency of redox interactions of the photoreduced primary electron acceptor with secondary acceptors and photooxidized bacteriochlorophyll has a marked and reversible dependence on temperature over the range from -20 degrees to -80 degrees. similar temperature dependences were observed for correlation times of the rotational diffusion of a spin probe bound to the hydrophobic region of the membrane and of a spin label bound to sh ...	1979	111034
purification and properties of nitrogenase from the cyanobacterium, anabaena cylindrica.	the nitrogenase complex was isolated from nitrogen-starved cultures of anabaema cylindrica. sodium dithionite, photochemically reduced ferredoxin, and nadph were found to be effective election donors to nitro genase in crude extracts whereas hydrogen and pyruvate were not. the km for acetylene in vivo is ten-fold higher than the km in vitro, whereas this pattern does not hold for the non-heterocystous cyanobacterium, plectonema boryanum. this indicates that at least one mechanism of oxygen prote ...	1979	111934
[effect of nitrogen-containing compounds on hydrogen light emission and nitrogen fixation by purple bacteria].	the cells of rhodospirillum rubrum and thiocapsa roseopersicina grown in media containing glutamate and arginine, respectively, as well as under conditions of nitrogen fixation evolve h2 in the light. if the cultures were grown in media with nh4+, no3-, urea, glutamine or asparagine, hydrogen photoevolution by the cells and acetylene reduction started after the lag-phase and proceeded at a low rate. extracts of such cells did not display the activity of nitrogenase which could be assayed by the ...	1979	112358
[use of urea by purple bacteria].	strains of purple sulfur bacteria (chromatium minutissimum, ectothiorhodospira shaposhnikovii, thiocapsa roseopersicina, lamprobacter modestohalophilus) and nonsulfur bacteria (rhodopseudomonas palustris, rh. spheroides, rhodospirillum rubrum) grow in media containing urea as a source of nitrogen at concentrations from 0.5 to 5.0%. they can also utilize the carbon of urea and thus grow in the absence of bicarbonate. urea is decomposed by all the studied purple bacteria with the participation of ...	1979	112359
[application of polarography to studies on redox systems in bio-membranes: especially on photosynthetic electron transport system in chromatophore membrane from photosynthetic bacterium (author's transl)].		1979	112648
the influence of energy-transfer inhibitors on proton permeability and photophosphorylation in normal and preilluminated rhodospirillum rubrum chromatophores.	(1) chromatophores were preilluminated in the presence of phenazine methosulphate or diaminodurene, and without phosphorylation substrates; next they were transferred to fresh medium and assayed for light-induced proton uptake, light-induced 9-aminoacridin fluorescence quenching, and photophosphorylation. (2) preillumination in the presence of phenazine methosulphate or diaminodurene causes an inhibition of the photophosphorylation rate. the presence of adp + mgcl2 + phosphate, or adp + mgcl2 + ...	1979	37903
photoreaction center of photosynthetic bacteria. 1. further chemical characterization of the photoreaction center from rhodospirillum rubrum.	the photoreaction center from rhodospirillum rubrum contains about 90% protein, 6% pigment, mere traces of lipids, and no cytochromes. it also contains at least 1 mol of ubiquinone and 1 iron atom per mol. its three-component polypeptide chains were isolated by preparative electrophoresis, and their molar stoichiometry was established as 1:1:1. the amino acid composition of the photoreaction center from strain s1 and from its subunits is reported. the protein as a whole contains about 65% nonpol ...	1979	114212
photoreaction center of photosynthetic bacteria. 2. size and quaternary structure of the photoreaction centers from rhodospirillum rubrum strain g9 and from rhodopseudomonas sphaeroides strain 2.4.1.	the photoreaction center from rhodospirillum rubrum strain g9 binds about 6 times as much sodium dodecyl sulfate as certain proteins commonly used as molecular weight markers for sodium dodecyl sulfate--polyacrylamide gel electrophoresis. this presumably explains the apparent discrepancy between the molecular weight of the photoreaction center determined by electrophoresis (76 000) and its minimal molecular weight (87 000). the molecular weight of the photoreaction center solubilized with triton ...	1979	114213
role of the large and small subunits of ribulose-1,5-bisphosphate carboxylase in the activation by co2 and mg2+.		1979	37245
a quantitative assay for bacterial rna polymerases.		1979	114520
activation and regulation of ribulose bisphosphate carboxylase-oxygenase in the absence of small subunits.	ribulose 1,5-bisphosphate carboxylase from rhodospirillum rubrum requires co2 and mg2+ for activation of both co2, both the carboxylase and oxygenase activities are stimulated by 6-phoshpo-d-gluconate, fructose 1,6-bisphosphate, 2-phosphoglycolate, 3-phosphoglycerate, nadph, and fructose 6-phosphate. the carboxylase activity is not activated by ribose 5-phosphate. the substrate, ribulose bisphosphate, neither activates nor inhibits the co2 and mg2+ activation of this enzyme. activation by co2 an ...	1979	114521
lipopolysaccharides of photosynthetic prokaryotes.		1979	115382
changes in the acyl lipid composition of photosynthetic bacteria grown under photosynthetic and non-photosynthetic conditions.	the acyl lipids and their constituent fatty acids were studied in the photosynthetic bacteria rhodospirillum rubrum, rhodopseudomonas capsulata and rhodopseudomonas sphaeroides, which were grown under photosynthetic and non-photosynthetic conditions. the major lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and cardiolipin in each bacterium. the two rhodopseudomonas species also contained significant quantities of phosphatidylcholine. other acyl lipids accounted for less t ...	1979	115463
differential effects of metal ions on rhodospirillum rubrum ribulosebisphosphate carboxylase/oxygenase and stoichiometric incorporation of hco3- into a cobalt(iii)--enzyme complex.	mg2+ or mn2+ ions supported both the carboxylase and oxygenase activities of the rhodospirillum rubrum ribulosebisphosphate carboxylase/oxygenase. for the carboxylase reaction, mn2+ supported 25% of the maximum activity obtained with mg2+; oxygenase activity, however, was twice as great with mn2+ as compared to that with mg2+. a further differential effect was obtained with co2+. co2+ did not support carboxylase activity and, in fact, was a strong inhibitor of mg2+-dependent carboxylase activity ...	1979	115489
extracellular hydrogenase from photosynthetic bacterium, rhodospirillum rubrum.	with rhodospirillum rubrum, hydrogenase was found to exist partly as an extracellular enzyme in the culture medium. after 4-day cultivation, the total activity and the specific activity of the enzyme in the medium were about 10 times and 230 times as high as those in the crude extract obtained from disrupted cells. the time course for the production of hydrogenase during cultivation was studied.	1979	115852
two regimens of electrogenic cyclic redox chain operation in chromatophores of non-sulfur purple bacteria. a study using antimycin a.	antimycin a causes a biphasic suppression of the light-induced membrane potential generation in rhodospirillum rubrum and rhodopseudomonas sphaeroides chromatophores incubated anerobically. the first phase is observed at low antibiotic concentrations and is apparently due to its action as a cyclic electron transfer inhibitor. the second phase is manifested at concentrations which are greater than 1--2 mum and is due to uncoupling that may be connected with an antibiotic-induced dissipation of th ...	1979	116681
short-lived delayed luminescence of photosynthetic organisms. i. nanosecond afterglows in purple bacteria at low redox potentials.	a combined study of emissions of purple bacteria rhodospirillum rubrum, ectothiorhodospira shaposhnikovii and thiocapsa roseopersicina was performed under conditions of low potential. it has been shown that a considerable part of the emission represents a delayed luminescence with a lifetime of about 5 ns and an activation energy delta e = 0.05 +/- 0.03 ev. intensity of this delayed luminescence is approximately equal to that of prompt fluorescence. it diminishes as temperature decreases and als ...	1979	116682
modification of ribulose bisphosphate carboxylase from rhodospirillum rubrum with tetranitromethane.		1979	110331
hydrophobic membrane protein from chromatophores of rhodospirillum rubrum. structural and spectroscopic studies of monolayers and multilayers.	a hydrophobic, lipid- and pigment-free polypeptide from the chromatophore membrane of rhodospirillum rubrum was spread from chloroform/methanol, pyridine and formic acid solutions at an air-water interface. surface pressure versus area isotherms of the monolayers formed at the interface were partially dependent upon the spreading solvent used. from the surface area at 20 dynes/cm compression, an average molecular area of 12.9 nm2/molecule was calculated for a polypeptide monolayer spread from ch ...	1979	110346
[noncyclic electron transport and membrane potential generation in the chromatophores of rhodospirillum rubrum].		1979	110361
removal of an adenine-like molecule during activation of dinitrogenase reductase from rhodospirillum rubrum.	during the activation of the inactive dinitrogenase reductase from rhodospirillum rubrum, an adenine-like molecules is lost and phosphate is found on both active and inactive forms of the protein. atp and divalent metals are required for activation of the reduced protein, but atp is not required for activation of phenazine methosulfate-oxidized dinitrogenase reductase. snake venom diesterase and spleen diesterase have no effect on the inactive protein; alkaline phosphatase removes phosphate from ...	1979	118462
purification, subunit structure, and kinetics of the chloroform-released f1atpase complex from rhodospirillum rubrum and its comparison with f1atpase forms isolated by other methods.	a stable and homogeneous adenosine-5'-triphosphatase (atpase, ec 3.6.1.3) has been solubilized from rhodospirillum rubrum (r. rubrum) chromatophores by chloroform extraction. purification of the ca2+-dependent atpase activity was 200-fold. ca2+ can be replaced by mg2+, cd2+, and mn2+. the km for ca-atp (0.17 mm) is increased about 5-fold during solubilization of the enzyme, whereas the km values for mg-atp (0.029 mm) and cd-atp (0.014 mm) are not affected. the chloroform-released atpase has a mo ...	1979	155949
coupling factor atpase complex of rhodospirillum rubrum. purification and characterization of an oligomycin and n,n'-dicyclohexylcarbodiimide-sensitive (ca+ + mg2+)-atpase.	an atpase complex sensitive to the energy transfer inhibitors oligomycin, dicyclohexylcarbodiimide and venturicidin has been solubilized from rhodospirillum rubrum chromatophores with triton x-100 and further purified by centrifugation on a glycerol gradient. the partially purified rrfo . f1 contains 13 distinct polypeptide subunits, as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, including the subunits of the oligomycin-sensitive, water-soluble rrf1 atpase. the atpase ...	1979	158383
[functional organization of the electron transport chain of rhodospirillum rubrum chromatophores in the absence of an exogenous electron donor].		1979	113038
nitrogenase from rhodospirillum rubrum. relation between 'switch-off' effect and the membrane component. hydrogen production and acetylene reduction with different nitrogenase component ratios.	nitrogenase activity of 'membrane-free' extracts, produced from nitrogen-starved rhodospirillum rubrum to which 4 mm nh4+ had been added is only about 10% of the activity in the control. the activity could be restored to 80% by including the membrane component, earlier found to activate r. rubrum nitrogenase, in the reaction mixture. the relation between this 'switch-off/switch-on' effect and the function of the membrane component is discussed. hydrogen production catalyzed by r. rubrum nitrogen ...	1979	114217
disintegration of rhodospirillum rubrum chromatophore membrane into photoreaction units, reaction centers, and ubiquinone-10 protein with mixture of cholate and deoxycholate.	1. the membrane of rhodospirillum rubrum chromatophores was disintegrated with mild detergents (cholate and deoxycholate) in order to study the spatial arrangement of the functional proteins in the photochemical apparatus and the electron transport system in the membrane. 2. the components solubilized from the membrane by a mixture of cholate and deoxycholate (c-doc) were separated into four fractions by molecular-sieve chromatography in the presence of c-doc; they were designated as f1, f2, f3, ...	1979	118165
properties of the f0f1 atpase complex from rhodospirillum rubrum chromatophores, solubilized by triton x-100.	1. a cold-stable oligomycin-sensitive f0f1 atpase complex from chromatophores of rhodospirillum rubrum fr 1 was solubilized by triton x-100 and purified by gel filtration. 2. the f0f1 complex is resolved by sodium dodecyl sulfate electrophoresis into 14 polypeptides with approximate molecular weights in the range of 58000--6800; five of these polypeptides are derived from the f1 moiety of the complex which carries the catalytic centers of the enzyme. 3. the purified f0f1 complex is homogeneous a ...	1979	157277
preparation of the soluble atpase from mitochondria, chloroplasts, and bacteria by the chloroform technique.		1979	156841
comparative solvent perturbation of horse heart cytochrome c and rhodospirillum rubrum cytochrome c2.	the extent of exposure of heme to solvent in horse heart cytochrome c and rhodospirillum rubrum c2 was investigated to determine whether a correlation exists between the properties of these oxidation-reduction proteins and their heme environments. solvent perturbation absorption difference spectra were measured using ethylene glycol, glycerol, and sucrose at concentrations between 0 and 30%. cytochrome c appears to exhibit a somewhat greater extent of heme exposure than cytochrome c2 for both th ...	1979	220233
immunological and reconstitution studies on the adenosine triphosphatase complex from rhodospirillum rubrum.	studies on restoration of membrane-bound adenosinetriphosphatase (atp phosphohydrolase, ec 3.6.1.3) from rhodospirillum rubrum show that the delta-subunit is capable of binding to the f1 factor or to the f0 moiety of the f0-f1 atpase complex. this subunit is thus likely involved in linking the f0 and f1 factor. during solubilization of the oligomycin-sensitive f0-f1 atpase complex with triton x-100 the detergent becomes specifically associated with the lipophilic f0 part of the enzyme complex. c ...	1979	153155
bound nucleotides and phosphorylation in rhodospirillum rubrum.		1979	155454
hydrogen production by the photosynthetic bacterium rhodospirillum rubrum.	continuous photosynthetic production of hydrogen by rhodospirillum rubrum in batch cultures was observed up to 80 days with the hydrogen donor, pure lactate or lactic acid-containing wastes, supplied periodically. hydrogen was produced at an average rate of 6 ml/h per g (dry weight) of cells with whey as a hydrogen donor. in continuous cultures with glutamate as a growth-limiting nitrogen source and lactate as a hydrogen donor, hydrogen was evolved at a rate of 20 ml/h per g (dry weight). the co ...	1979	16345375
c nuclear magnetic resonance study of the co(2) activation of ribulosebisphosphate carboxylase from rhodospirillum rubrum.	ribulosebisphosphate carboxylase [3-phospho-d-glycerate carboxy-lyase (dimerizing), ec 4.1.1.39] from rhodospirillum rubrum is activated by co(2) and mg(2+). (13)c nmr spectra were determined for the unactivated enzyme and for enzyme that had been activated by (13)co(2) and mg(2+). in addition to the expected resonance for h(13)co(3) (-)/co(3) (2-) at 161.8 ppm downfield from tetramethylsilane, the spectrum of the activated enzyme shows a broad resonance at 164.9 ppm. analogy with previous nmr s ...	1979	16592618
carbon isotope fractionation by ribulose-1,5-bisophosphate carboxylase from various organisms.	carbon isotope fractionation by structurally and catalytically distinct ribulose-1,5-bisphosphate carboxylases from one eucaryotic and four procaryotic organisms has been measured under nitrogen. the average fractionation for 40 experiments was -34.1 per thousand with respect to the delta(13)c of the dissolved co(2) used, although average fractionations for each enzyme varied slightly: spinach carboxylase, -36.5 per thousand; hydrogenomonas eutropha, -38.7 per thousand; agmenellum quadruplicatum ...	1978	16660363
mössbauer study of cytochrome c2 from rhodospirillum rubrum. sign of the product gxgygz of some low spin ferric heme proteins.	we have studied cytochrome c2 from rhodospirllum rubrum with mössbauer spectroscopy and electron paramagnetic resonance. the mössbauer data on the ferric protein, taken in external magnetic fields up to 50 kg, were analyzed within the framework of the ligand field model commonly used to evaluate low-spin ferric heme compounds. the data analysis shows that the determinant of the electronic g-tensor, i.e. the product gxgygz, is positive for cytochrome c2. we have reanalyzed published mössbauer dat ...	1978	208633
flash-induced changes in the in vivo bacteriochlorophyll fluorescence yield at low temperatures and low redox potentials in carotenoid-containing strains of photosynthetic bacteria.	the changes in the in vivo bacteriochlorophyll fluorescence induced by a xenon flash at low temperatures (77--200 k) with the "primary" acceptor x chemically prereduced have been examined in whole cells of several species of photosynthetic bacteria which contain carotenoids absorbing in the visible part of the absorption spectrum. two groups of species with different behaviour could be distinguished. in both cases a flash-induced rise of the fluorescence yield was observed with x prereduced at 7 ...	1978	96856
relationships in hydrogen metabolism between hydrogenase and nitrogenase in phototrophic bacteria.	purple bacteria rhodospirillum rubrum and thiocapsa roseopersicina form two enzymes, hydrogenase and nitrogenase, which participate in hydrogen metabolism. h2 photoproduction in these bacteria is associated mainly or completely with the action of nitrogenase. the soluble and membrane-bound hydrogenases of t. roseopersicina have similar physicochemical properties (mol. weight, subunit composition, n-terminal amino acids, fe2+ and s2- content, pl. eo'). in comparison with other hydrogenases the en ...	1978	208659
on the significance of electron transport systems for growth of rhodospirillum rubrum.		1978	211972
phenazine methosulfate mediated photoinactivation of some energy linked reactions in rhodospirillum rubrum.		1978	208532
facile assay of enzymes unique to the calvin cycle in intact cells, with special reference to ribulose 1,5-bisphosphate carboxylase.		1978	204219
the interactions of coupling atpases with nucleotides.		1978	147104