Publications

[subgingival distribution of periodontopathic bacteria in periodontic patients and susceptibility of these bacteria to minocycline-hcl].	the present study was carried out to examine the distribution of six periodontopathic bacteria in deep periodontal pockets and to reconfirm the effect of periocline on these periodontopathic bacteria. samples from sixty-two periodontal pockets were collected at pocket depths of over 4 mm in twenty-one periodontitis patients aged 43 to 75 years. after sampling, periocline was applied topically to the selected pockets once a week for four weeks and reexamined. the detected rates of the periodontop ...	1995	8583161
effect of whole oral bacteria and extracted lipopolysaccharides on peripheral blood leukocyte interleukin-2 receptor expression.	expression of the interleukin-2 receptor (il-2r) on t cells is the molecular mechanism that initiates the g0 to g1 transition and is the critical first step for t cell proliferation in response to antigen. the effect of whole periodontal bacteria and lipopolysaccharides (lps) on peripheral blood mononuclear cell (pbmc) il-2r expression was examined in vitro. lps induced a modest but significant increase in high affinity il-2r alpha/beta (p55/p75 positive) expression on pbmc over untreated cells ...	1995	7562323
periodontal status and selected cultivable anaerobic microflora of insulin-dependent juvenile diabetics.	the periodontal status and subgingival microflora of insulin-dependent juvenile diabetic (jd) patients (n = 16, mean age = 11.3) were compared with that of their non-diabetic cohabiting healthy siblings (hs, n = 16, mean age = 13.2). jd patients were monitored every 3 months for levels of glycosylated hemoglobin (hba1c) and clinical and microbial parameters were measured 6 weeks before drawing blood for levels of hba1c (m% = 8.76). clinical indices, measured for the entire permanent dentition, i ...	1995	7562335
clinical, microbiological and host defense parameters associated with a case of localized prepubertal periodontitis.	a 4-year-old japanese boy was referred to osaka university dental hospital because of severe mobility and pain of the right lower primary canine. the canine had severe bone loss and a pocket depth exceeding 5-6 mm. the left lower canine showed slight mobility and moderate alveolar bone loss. the other primary teeth showed no pathogenic findings. the subgingival microflora from the right lower canine was dominated by gram-negative rods, especially capnocytophaga and fusobacterium, while actinomyc ...	1995	7706540
capnocytophaga canimorsus septicemia: fifth report of a cat-associated infection and five other cases.	capnocytophaga canimorsus is a fastidious, slow-growing, gram-negative, rod-shaped bacterium that belongs to the normal oral flora of dogs and cats. human septicemic infections are associated with a high mortality; most cases occur in immunocompromised patients with a history of dog bite. the fifth case of cat-associated septicemia caused by capnocytophaga canimorsus is described. the six case reports presented here point out the characteristics reported previously: (a) cats are a source of huma ...	1995	7588825
[aortic endocarditis due to capnocytophaga sputigena].		1995	7603114
intracellular multiplication and toxic destruction of cultured macrophages by capnocytophaga canimorsus.	capnocytophaga canimorsus is a gram-negative rod that causes opportunistic infections resulting in bacteremia, septicemia, meningitis, and death in immunocompromised, splenectomized, and alcoholic individuals. infections caused by a related species, capnocytophaga cynodegmi, remain localized at the site of the wound where the organism is introduced. both organisms are part of the normal canine oral flora and are introduced through puncture wounds via dog bites. we found that both c. canimorsus a ...	1995	7642281
capnocytophaga (capnocytophaga ochracea group) bacteremia in hematological patients with profound granulocytopenia.	the clinical and microbiological features of 7 cases of bacteremia due to capnocytophaga (capnocytophaga ochracea group) are reported. they were diagnosed during 1991-93 at three hospital clinics. five patients were < 10 years old and all had hematological disorders, 4 acute lymphoblastic leukemia and 1 each had aplastic anemia, non-hodgkin lymphoma, and myelodysplastic syndrome. all were profoundly granulocytopenic with an absolute granulocyte count < 0.13 x 10(9)/l, and all but 1 had oral lesi ...	1995	7660080
[conjunctivitis caused by capnocytophaga ochracea in a 2-year-old girl].		1995	7779886
chorioamnionitis caused by capnocytophaga: case report.	intraamniotic infection is a significant cause of perinatal morbidity. capnocytophaga, a gram-negative anaerobe found in the oral cavity, has been implicated as an unusual cause of neonatal sepsis. we report a patient in whom this unusual organism was identified and describe the postpartum and neonatal courses.	1995	7631698
comparison of randomly cloned and whole genomic dna probes for the detection of porphyromonas gingivalis and bacteroides forsythus.	whole genomic and randomly-cloned dna probes for two fastidious periodontal pathogens, porphyromonas gingivalis and bacteroides forsythus were labeled with digoxigenin and detected by a colorimetric method. the specificity and sensitivity of the whole genomic and cloned probes were compared. the cloned probes were highly specific compared to the whole genomic probes. a significant degree of cross-reactivity with bacteroides species, capnocytophaga sp. and prevotella sp. was observed with the who ...	1996	8636873
histological and microbiological aspects of ligature-induced periodontitis in beagle dogs.	this study was designed to investigate, using 6 beagle dogs, the levels of selected putative pathogens in healthy sites, in gingivitis sites, and in sites with histologically confirmed attachment loss. levels of attachment loss increased with increasing periods of ligation and reached a maximum of 0.15 mm at 57 days. both histological attachment level and histological pocket depth were found to vary significantly with health/disease status (p < 0.0001). higher numbers of total colony-forming uni ...	1996	8739161
porphyromonas gingivalis, actinobacillus actinomycetemcomitans and treponema denticola detection in oral plaque samples using the polymerase chain reaction.	detection of putative pathogens is critical for delineating the etiology and progression of periodontitis. in the present study, we have used a polymerase chain reaction (pcr) assay utilizing primers specific for the lkt a gene of actinobacillus actinomycetemcomitans, the fimbrial gene of porphyromonas gingivalis, and tdp a gene of treponema denticola in order to determine the presence of these pathogens in subgingival plaque samples from periodontitis sites. these gene specific primers were als ...	1996	8707980
prevalence of 6 putative periodontal pathogens in subgingival plaque samples from romanian adult periodontitis patients.	the aim of the present study was to determine by standard cultivation procedures the detection frequencies of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, bacteroides forsythus, and capnocytophaga species as well as various enteric rods in subgingival plaque samples form romanian adult periodontitis patients. dna probe analysis (affirm dp microbial identification test) was also used, parallel to cultivation, to identify p. gingiv ...	1996	8849850
rapid characterization of periodontal bacterial isolates by using fluorogenic substrate tests.	eighty-nine species of subgingival bacteria, represented by 121 reference strains and 892 patient isolates, including gram-negative, gram-positive, aerobic, facultatively anaerobic, microaerophilic, and anaerobic species, were characterized with a panel of fluorogenic, 4-methylumbelliferyl-linked substrate tests. identifications of all patient isolates were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of whole-cell proteins relative to reference strains. char ...	1996	8789019
chemiluminescent assay of alkaline phosphatase in human gingival crevicular fluid: investigations with an experimental gingivitis model and studies on the source of the enzyme within crevicular fluid.	the purpose of this study was to investigate how levels of gingival crevicular fluid (gcf) alkaline phosphatase (alp) change in relation to levels of plaque and gingival inflammation in 20 adults during a 21 day period of experimental gingivitis. the source of alp within gcf was also investigated using a repeat sampling protocol; by determining enzyme levels derived from 30 putative periodontal pathogenic and non-pathogenic species; and by examining inhibition profiles from a variety of host and ...	1996	8811480
bacteriologic findings in tonsillitis and pericoronitis.	bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ...	1996	8816129
degradation of lactoferrin by periodontitis-associated bacteria.	the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ...	1996	8961558
novel fnr homologues identified in four representative oral facultative anaerobes: capnocytophaga ochracea, capnocytophaga sputigena, haemophilus aphrophilus, and actinobacillus actinomycetemcomitans.	based upon dna sequence data and positive immunochemical reactivity of expressed protein, novel homologues of the fnr family were identified in four representative oral facultative anaerobes: capnocytophaga ochracea, capnocytophaga sputigena, haemophilus aphrophilus, and actinobacillus actinomycetemcomitans. the similarity to e. coli fnr and to hlyx (itself 71% similar to e. coli fnr, while regulating expression of hemolysin operon in actinobacillus pleuropneumoniae) was estimated from the deduc ...	1996	8998988
plaque ph and microflora of dental plaque on sound and carious root surfaces.	given the hypothesis that root caries is the result of acid formation by acidogenic micro-organisms, the present study was performed to relate sucrose-induced ph response of dental plaque on root surfaces to the microbial composition of the overlying plaque. seventeen caries-active elderly chinese with poor oral hygiene and with both sound and carious root surfaces were examined. plaque ph was measured before and up to one hour after a controlled sucrose mouthrinse. plaque samples for microbiolo ...	1996	9003238
differentiation of human capnocytophaga species by multilocus enzyme electrophoretic analysis and serotyping of immunoglobulin a1 proteases.	as part of a larger taxonomic investigation of the genus capnocytophaga, 50 strains, including reference strains as well as clinical isolates, were subjected to multilocus enzyme electrophoretic (mlee) analysis of 12 intracellular metabolic enzymes and characterization of their immunoglobulin a1 (iga1) proteases by enzyme-neutralizing antibodies raised in rabbits. the dendrogram derived from cluster analysis of the mlee data discriminated between the five known human capnocytophaga species and s ...	1996	8932716
degradation of host protease inhibitors and activation of plasminogen by proteolytic enzymes from porphyromonas gingivalis and treponema denticola.	bacterial proteases may participate in the pathogenesis of periodontal diseases through their action on host proteins. in the present study, the ability of selected periodontopathogens, as well as two proteases isolated from porphyromonas gingivalis and treponema denticola, to degrade host protease inhibitors was evaluated. the activation of human plasminogen by the two bacterial proteases was also investigated. proteolytic breakdown of host protease inhibitors (alpha-1-antitrypsin, antichymotry ...	1996	8936322
pcr reaction and dot-blot hybridization to monitor the distribution of oral pathogens within plaque samples of periodontally healthy individuals.	the purpose of this study was to determine the distribution of the putative periodontal pathogens prevotella intermedia, prevotella nigrescens, the three oral capnocytophaga species (c. ochracea, c. sputigena, c. gingivalis), as well as porphyromonas gingivalis and actinobacillus actinomycetemcomitans in plaque samples of periodontally healthy individuals. we chose a newly developed 16s rdna directed polymerase chain reaction (pcr) and a previously described dot-blot hybridization assay to detec ...	1996	8910839
[capnocytophaga canimorsus bacteremia].	a case of bacteraemia in a 54-year-old previously healthy man caused by the gram-negative rod capnocytophaga canimorsus is presented. the patient developed severe ischaemia in both feet, thrombocytopenic purpura and renal failure. the bacteraemia was apparently caused by saliva from the patient's dog who had licked ulcers on the patient's legs. the patient regained full health but had a minor inferior myocardial infarction seven weeks after start of the infection.	1996	8685995
[bacteremia caused by capnocytophaga sp: presentation of 2 cases, one with endocarditis. review of the literature].	capnocytophaga sp. is a gram-negative bacilli, scarcely documented as the cause of bacteremias. two cases of bacteremia caused by capnocytophaga sp, one of them with endocarditis, are reported here. a review of previous published cases is also presented. one of the patients was immunocompromised, because of chemotherapy, the other, suffered from a rheumatic-cardiopathy which was complicated with endocarditis. both patients developed an alteration of the oral mucosa. antibiotic therapy proved to ...	1996	8688478
rheumatoid factor from periodontitis patients cross-reacts with epitopes on oral bacteria.	the objective of this study was to determine the antigenic specificity of rheumatoid factor (rf) that had previously been reported in the serum of patients with periodontitis.	1996	9171508
capnocytophaga canimorsus endophthalmitis.		1996	8724964
capnocytophaga gingivalis: effects of glucose concentration on growth and hydrolytic enzyme production.	in chemostat culture, the microaerophilic, co2 requiring, gingival-plaque-associated bacterium capnocytophaga gingivalis responded to the addition of glucose (1-6 g i-1) by doubling its growth rate and increasing its biomass yield fivefold. the data suggest that the glucose is catabolized by a fully aerobic route. rather than repressing hydrolytic enzymes which might be associated with pathogenic properties, glucose enhanced the specific activity of aminopeptidase, trypsin-like protease, acid an ...	1996	8760930
polyphasic analysis of strains of the genus capnocytophaga and centers for disease control group df-3.	a polyphasic approach was used to determine the relationships between well-characterized reference strains representing all seven capnocytophaga species. one centers for disease control (cdc) group df-3 strain, a presumed relative of the genus capnocytophaga, and 15 field isolates were included as well. fourteen isolates were assigned to named capnocytophaga species, all of which could be differentiated by means of whole-organism protein electrophoresis. a separate position was occupied by the c ...	1996	8782690
beware of the dog! a syndrome resembling thrombotic thrombocytopenic purpura associated with capnocytophaga canimorsus septicaemia.		1996	8918633
effects of sublethal exposure to an antiseptic mouthrinse on representative plaque bacteria.	although the mechanism responsible for the clinical antiplaque efficacy of oral antiseptics is generally considered to be primarily one of bactericidal activity, it has been suggested that oral antiseptics may have additional effects on bacteria exposed to sublethal levels. studies reported herein, investigated the effects of sublethal levels of an essential oil-containing antiseptic mouthrinse (listerine antiseptic, warner-lambert co., morris plains, nj) on selected activities of representative ...	1996	8783049
vertebral osteomyelitis due to capnocytophaga species in immunocompetent patients: report of two cases and review.	vertebral osteomyelitis caused by capnocytophaga species was diagnosed in two adults. one of these infections was due to c. ochracea and the other was due to c. sputigena. both patients were immunocompetent and presented with concomitant periodontitis and gingivitis. the infections were eradicated by means of prolonged antibiotic therapy, combined in one case with surgical treatment. capnocytophaga species should be considered a potential cause of vertebral osteomyelitis in immunocompetent patie ...	1996	8783720
capnocytophaga canimorsus septicemia in denmark, 1982-1995: review of 39 cases.	thirty-nine danish cases of capnocytophaga canimorsus septicemia were reviewed to determine the clinical course of this infection. the cases of septicemia were related to recent dog bites or other close contact with dogs. the period from the bite to the onset of symptoms ranged from 1 to 8 days. the mean age of the patients was 59.1 years (range, 28-83 years). underlying conditions included previous splenectomy and alcoholism. thirteen patients had previously been in good health. common initial ...	1996	8816132
capnocytophaga meningitis in a cancer patient.		1996	8823865
the dynamics of microbial colonization of barrier membranes for guided tissue regeneration.	the microbial colonization of expanded polytetrafluoroethylene membrane by putative periodontopathogens at 3 minutes of intraoral manipulation was determined in 42 patients with 42 mandibular posterior two- to three-wall defects. twenty patients exhibited no periodontal pockets of > or = 5 mm, other than the study site, and low levels of pathogens (group a). twenty-two patients revealed multiple periodontal pockets of 5 mm or more and numerous pathogens (group b). within the preceding 3 months o ...	1996	8832481
capnocytophaga chorioamnionitis after oral sex.		1996	8841277
capnocytophaga canimorsus sepsis complicated by myocardial infarction in two patients with normal coronary arteries.	we describe two patients who had acute myocardial infarctions during episodes of capnocytophaga canimorsus sepsis. c. canimorsus is associated with severe infection in patients who are immunocompromised; one of these patients had undergone splenectomy for hodgkin's disease 11 years earlier, and the other consumed significant amounts of alcohol regularly. both patients owned dogs that had licked them or produced minor skin wounds shortly before they became ill. coronary angiographic findings were ...	1996	8842273
capnocytophaga canimorsus infections in human: review of the literature and cases report.	capnocytophaga canimorsus, formerly designated dysgonic fermenter 2 (df-2) was first described in 1976; it is a commensal bacterium of dogs and cats saliva, which can be transmitted to man by bite (54% of cases), scratch (8.5%), or mere exposure to animals (27%). we present a review of the clinical and microbiological characteristics of the capnocytophaga canimorsus infections and 12 cases of infection in france. over 100 cases of human infections have been reported, mainly septicemia in patient ...	1996	8905316
hydrolysis of triclosan monophosphate by dental plaque and selected species of oral micro-organisms.	triclosan monophosphate is a phosphorylated derivative of the antimicrobial agent, triclosan. in comparison with triclosan, it is highly soluble in aqueous solutions. it is hypothesized that, within the oral environment, triclosan monophosphate (which may be devoid of antimicrobial activity) will be hydrolyzed into triclosan by the action of microbial phosphatases. the liberated triclosan may then exert antimicrobial activity. to test this hypothesis, we designed experiments to measure the phosp ...	1996	8906126
predominant cultivable supragingival plaque in chinese "rapid" and "slow" plaque formers.	the aim of this study was to compare the predominant cultivable bacterial flora in supragingival plaque samples of chinese "rapid" and "slow" plaque formers, using the experimental gingivitis model. 11 chinese subjects (5 "rapid" and 6 "slow" plaque formers) were selected from 49 healthy young adults. the selection was based on the plaque index on the buccal surfaces of all the canines, premolars and 1st molars after 3-days without plaque control. the 11 subjects began a 14-day on oral hygiene p ...	1996	8951632
serum rheumatoid factor induced by intraperitoneal administration of periodontopathic bacterial lipopolysaccharide in mice.	serum rheumatoid factor (rf) level and peritoneal and splenic cd5+b (b-1) cells in mice were examined after intraperitoneal administration of purified lipopoly-saccharides (lps) from oral periodontopathic bacteria; porphyromonas gingivalis, actinobacillus actinomycetemcomitans, fusobacterium nucleatum and capnocytophaga ochracea. f. nucleatum and c. ochracea lps induced higher levels of serum igm- and igg-rf, while p. gingivalis lps showed the least induction. in addition, wet weights of spleen ...	1996	8915954
predominant cultivable microflora of supragingival dental plaque in chinese individuals.	the aim of this study was to determine the predominant supragingival cultivable bacterial flora in chinese individuals, using the experimental gingivitis model. a total of 11 healthy dental students, mean age 22.5 years (range 20-25) were recruited. all were provided with once-a-week dental prophylaxis and oral hygiene reinforcement for 3 weeks to ensure gingival health. in the fourth week, after prophylaxis, the participants entered a 14-day period without any plaque control. a plaque sample wa ...	1996	9015565
effect of oral bacteria on peripheral blood leukocyte interleukin-6 and soluble interleukin-6 receptor production.	to determine the effect of pathogenic oral bacteria on interleukin 6 (il-6) and soluble il-6 receptor production, we measured their release by human peripheral blood mononuclear cells in vitro. unseparated peripheral blood mononuclear cells, peripheral blood lymphocytes (monocyte depleted), pure t cells, or monocytes were cultured with actinobacillus actinomycetemcomitans, capnocytophaga gingivalis, capnocytophaga ochracea, fusobacterium nucleatum or porphyromonas gingivalis for 24 h. supernatan ...	1996	9028259
the antimicrobial activity of endodontic sealers to anaerobic bacteria.	the effect of five root canal sealers and two root canal dressing materials on the growth of three anaerobic bacteria associated with endodontic infections was determined using the agar diffusion inhibitory test. samples of the following endodontic sealers (apexit, ketac-endo, roth sealer, sealapex and tubliseal) and root canal dressing materials (pulpdent and root-cal) were incubated for 48 h with the following anaerobic bacteria: capnocytophaga ochracea, porphyromonas gingivalis and peptostrep ...	1996	9206446
intra-amniotic infection caused by capnocytophaga species.	capnocytophaga species are common oral pathogens and infrequent causes of systemic infection in patients with compromised host. the isolation of this organism suggests an oral source of infection.	1996	18476112
[the study on sds-page technic classification of the whole protein of oral bacteria].	oral flora was classificated by sds-page combined with comassie blue staining and silver staining. the results showed that each species of the test oral bacteria expressed its specific electrophorectic protein pattern, the way of sample preparation, staining did not change the protein pattern. this indicated that sds-page could be used to classificate oral flora. the protein pattern was more obvious among the genus than that of within the genus. this showed that different relationship was among ...	1997	11480050
periodontal microflora of hiv infected patients with periodontitis.	the aim of this study was to determine the microbial profile of periodontal lesions in hiv seropositive patients and to compare it with rapidly progressing periodontal lesions in systemically healthy patients. the subgingival microflora of 20 cdc ii, 20 cdc iii, 20 cdc iv/v and 20 systemically healthy patients with rapidly progressing periodontitis was examined. four sites with greatest probing depth in each patient were selected for microbiological sampling. the samples were cultured aerobicall ...	1997	16887570
subgingival microflora in smokers with early onset periodontitis.	cigarette smoking is a potent risk factor which has recently been associated with periodontal disease progression. the objective of this study was to detect the microbial profile of early onset periodontitis in smokers and compare it to that of non-smokers. the study population consisted of 50 systemically healthy individuals aged 25 to 38 years, exhibiting early onset periodontitis. 25 patients were smokers (> 20 cigarettes/day) and 25 non-smokers. two pooled bacterial samples comprised of four ...	1997	16887581
differential induction of pro- and anti-inflammatory cytokines in whole blood by bacteria: effects of antibiotic treatment.	the in vitro production of interleukin-1beta (il-1beta), il-6, and the il-1 receptor antagonist (il-1ra) in whole blood upon stimulation with different bacterial strains was measured to study the possible relationship between disease severity and the cytokine-inducing capacities of these strains. escherichia coli, neisseria meningitidis, neisseria gonorrhoeae, bacteroides fragilis, capnocytophaga canimorsus, staphylococcus aureus, enterococcus faecalis, streptococcus pneumoniae, and streptococcu ...	1997	9210662
sensitivity of periodontal pathogens to the bactericidal activity of synthetic protegrins, antibiotic peptides derived from porcine leukocytes.	protegrins, small peptides (1900 to 2160 daltons) isolated from porcine leukocytes, are bactericidal against a broad range of medical pathogens in vitro under conditions which reflect the extracellular milieu. the purpose of this study was to determine whether gram-negative, facultative periodontal pathogens were sensitive to the protegrins. synthetic l- and d-enantiomers of protegrin 1 (pg-1 and d-pg-1, respectively), and l-enantiomers of protegrins 2, 3, and 5 (pg-2, pg-3, and pg-5) were teste ...	1997	9240381
liver abscess caused by capnocytophaga species.		1997	9243050
capnocytophaga canimorsus septicemia in an asplenic patient.	we present a classic case of capnocytophaga canimorsus septicemia. c. canimorsus (from the latin canis, dog; and morsus, a bite), formerly called cdc group df-2, is a gram-negative rod that typically causes septicemia with disseminated intravascular coagulation in both immunocompromised and immunocompetent hosts.	1997	9283773
clinical and microbiological features of subjects with adult periodontitis who responded poorly to scaling and root planing.	in a previous report, it was shown that scaling and root planing (srp) decreased mean pocket depth and attachment level in subjects with adult periodontitis, as well as the levels and prevalence of bacteroides forsythus, porphyromonas gingivalis and treponema denticola. however, a subset of subjects in that study exhibited mean loss of attachment following srp. the purpose of the present investigation was to seek clinical and microbiological differences between subjects who responded well or poo ...	1997	9350562
dna-probes for the differentiation of capnocytophaga species.	we designed oligonucleotides to differentiate between the seven currently known capnocytophaga species. the oligonucleotides were labelled non-radioactively at the 3' end with digoxigenin. the specificity could be demonstrated in a dot-blot hybridization assay by using the type strains, reference strains, and 37 clinical capnocytophaga isolates as well as 11 representative strains of other taxa as a template. the sensitivity of the assay was calculated with 10(3) bacteria per dot.	1997	9375291
secretion of il-1 beta, tnf-alpha, il-8 and il-1ra by human polymorphonuclear leukocytes in response to lipopolysaccharides from periodontopathic bacteria.	polymorphonuclear leukocytes (pmn) are the first cells that migrate into periodontal tissues and gingival crevices in response to invading pathogens. it was recently demonstrated that pmn have the ability to synthesize and release cytokines following appropriate stimulation, while it is not clear whether these capacities are directly related to periodontal destructive processes. we therefore investigated the amounts of the cytokines interleukin-1 beta (il-1 beta), tumor necrosis factor alpha (tn ...	1997	9138193
beware of the dog!		1997	9075160
diagnosis: capnocytophaga canimorsus septicemia.		1997	9114133
[bacteremia caused by capnocytophaga ochracea: apropos of 2 cases].		1997	9376412
microbial ecology of actinobacillus actinomycetemcomitans, eikenella corrodens and capnocytophaga spp. in adult periodontitis.	information on intraoral distribution of putative periodontal pathogens might be essential for controlling different forms of periodontal disease. colonization may be either promoted or impeded by other bacteria competing in the subgingival ecosystem. in recent investigations microbial associations between dental organisms have been determined in a multitude of subgingival plaque samples within multiple patients and described by odds ratios, in most circumstances without taking into account the ...	1997	9379321
man's best friend: life threatening sepsis after minor dog bite.		1997	9006476
effect of triclosan on the subgingival microbiota of periodontitis-susceptible subjects.	the present study evaluated the long-term effect of (i) meticulous self-performed, supragingival plaque control and (ii) the use of a triclosan/copolymer containing dentifrice in adult subjects susceptible to destructive periodontitis. 40 individuals were recruited into the trial. 3-5 years prior to the baseline examination, they had all been treated by nonsurgical means- for advanced periodontal disease. during the subsequent maintenance phase, all subjects had at different time intervals exhib ...	1997	9442424
oral food consumption and subgingival microorganisms: subgingival microbiota of gastrostomy tube-fed children and healthy controls.	this study examined the effect of oral food consumption on the prevalence and levels of subgingival bacteria and yeasts in 20 gastrostomy tube-fed children and 24 healthy controls. microbial identification was carried out using anaerobic culture and 16s rrna-based pcr identification methods. streptococcal and actinomyces species were recovered from 100% and 76% of all subjects and averaged 66% and 11% of total cultivable organisms, respectively. in decreasing order of prevalence, fusobacterium, ...	1997	9444590
extracts of prevotella intermedia and actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro.	in order to study the effects of sonicated extracts from prevotella intermedia, actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and other oral-related bacteria, as well as escherichia coli on bone formation, clone mc3t3-e1 cells, which have retained osteoblastic activity, were cultured with various bacterial extracts. the addition of the sonicated extracts from prevotella intermedia and actinobacillus actinomycetemcomitans decreased the alkaline phosphatase activity in a dose-dep ...	1997	9467351
characterization of protease activities in capnocytophaga spp., porphyromonas gingivalis, prevotella spp., treponema denticola and actinobacillus actinomycetemcomitans.	protease activities in cell sonicates of defined bacterial strains were examined using peptide substrates and class-specific inhibitors. capnocytophaga spp. all produced serine dipeptidyl peptidase activity and arginine/lysine, elastase- and chymotrypsin-like enzymes with some metalloprotease characteristics. the elastase-like activity was strongest in capnocytophaga sputigena, but the others were greatest in capnocytophaga gingivalis. the latter also had a separate arginine-specific enzyme whic ...	1997	9467394
comparative analysis of immunoglobulin a1 protease activity among bacteria representing different genera, species, and strains.	immunoglobulin a1 (iga1) proteases cleaving human iga1 in the hinge region are produced constitutively by a number of pathogens, including haemophilus influenzae, neisseria meningitidis, neisseria gonorrhoeae, and streptococcus pneumoniae, as well as by some members of the resident oropharyngeal flora. whereas iga1 proteases have been shown to interfere with the functions of iga antibodies in vitro, the exact role of these enzymes in the relationship of bacteria to a human host capable of respon ...	1997	9353019
inhibition of prevotella and capnocytophaga immunoglobulin a1 proteases by human serum.	oral prevotella and capnocytophaga species, regularly isolated from periodontal pockets and associated with extraoral infections, secret specific immunoglobulin a1 (iga1) proteases cleaving human iga1 in the hinge region into intact fab and fc fragments. to investigate whether these enzymes are subject to inhibition in vivo in humans, we tested 34 sera from periodontally diseased and healthy individuals in an enzyme-linked immunosorbent assay for the presence and titers of inhibition of seven pr ...	1997	9220164
microbial findings of deep peri-implant bone defects.	the objective of this study was to examine inflammatory tissue in deep peri-implant bone pockets (> 5 mm) for anaerobic bacteria colonization. the peri-implant inflammatory tissue of bone defects from 12 edentulous patients with 18 unsuccessful implants (imz type) was removed after surgical opening of the defects. after grinding the tissue with glass beads in nutrient solution, an aliquot of the suspension was plated and incubated on appropriate culture media. the quantitative and qualitative di ...	1997	9048462
myeloperoxidase isoform activities released by human neutrophils in response to dental and periodontal bacteria.	myeloperoxidase is an enzyme released by polymorphonuclear leukocytes which has been used, experimentally, as an indicator of periodontal disease activity when measured in gingival crevicular fluid. there are three myeloperoxidase isoforms: mpo i, mpo ii and mpo iii. we examined the activities of myeloperoxidase isoforms released by neutrophils in response to serum-opsonized actinobacillus actinomycetemcomitans, haemophilus aphrophilus, eikenella corrodens, capnocytophaga sputigena and streptoco ...	1997	9151641
effects of triclosan and triclosan monophosphate on maximum specific growth rates, biomass and hydrolytic enzyme production of streptococcus sanguis and capnocytophaga gingivalis in continuous culture.	dental plaque species, streptococcus sanguis and capnocytophaga gingivalis, were grown in continuous culture with progressively increasing concentrations of triclosan or its phosphorylated derivative, triclosan monophosphate (tmp). for both organisms, the maximum specific growth rates decreased with increasing concentrations of triclosan or tmp until complete inhibition of growth occurred, which for s. sanguis was at 20 mg/l and 50 mg/l, and for c. gingivalis was at 10 mg/l and 5 mg/l for triclo ...	1997	9421313
dog-bite induced sepsis: a report of four cases.	occasionally, a dog-bite is complicated by a systemic overwhelming infection. we report four consecutive patients who were admitted to our intensive care unit because of sepsis syndrome following dog-bites. the history of these patients did not reveal any immunocompromising conditions. capnocytophaga canimorsus (c. canimorsus) was cultured from the blood culture of 2 patients. our data illustrate that in patients with lack of immune-deficiency severe sepsis may develop.	1997	9434927
hla-d types and serum igg responses to capnocytophaga in diabetes and periodontitis.	serum igg responses to the cell envelope proteins (ceps) from capnocytophaga sputigena, capnocytophaga ochracea, and capnocytophaga gingivalis were examined in periodontally healthy and periodontitis subjects, both with and without type 1 diabetes (n = 60). serum igg responses to ceps were determined by immunoblotting with biotin-goat anti-human igg and an alkaline phosphatase-streptavidin system. reactivity was analyzed by transmission densitometry, digitization, and computer manipulation. the ...	1997	9390475
acute renal failure with hypocomplementaemic microangiopathy secondary to presumed capnocytophagia sepsis; response to fresh frozen plasma.		1997	9430908
[meningitis caused by capnocytophaga canimorsus].		1997	10622129
fulminant infection by uncommon organisms in animal bite wounds.	in 1995 and 1996, 215 patients exposed to different species of animals were treated at the amarnath polyclinic, balasore, in india. among them were two children infected by uncommon organisms, i.e., capnocytophaga canimorsus and pasteurella multocida; the patients recovered with appropriate antibiotic therapy.	1998	10211359
[bacterial infections as complications of dog bites].	dog bites may result in serious bacterial infections with e.g. the gram-negative rods capnocytophaga canimorsus and pasteurella multocida. human disease caused by these microorganisms can be complicated by acute development of septicaemia and/or meningitis followed by disseminated intravascular coagulation syndrome, peripheral gangrene and renal failure. the mortality of c. canimorsus septicaemia is about 23-31%. these severe infections are most often reported in immunocompromised patients and o ...	1998	9741251
[beware of the dog! a syndrome resembling thrombotic thrombocytopenic purpura associated with capnocytophaga canimorsus septicemia].		1998	9775193
capnocytophaga bacteremia: clinical features of patients and antimicrobial susceptibility of isolates.	capnocytophaga has been recognized as an opportunistic pathogen causing systemic infections in immunocompromised individuals with granulocytopenia and oral ulceration. treatment of capnocytophaga infection is often empiric. we retrospectively analyzed the clinical features of all patients with capnocytophaga bacteremia seen at the national taiwan university hospital between january 1981 and december 1996 and the antimicrobial susceptibility of the isolates recovered from these patients. all the ...	1998	9481064
capnocytophaga canimorsus in the oral flora of dogs and cats.		1998	9515691
[retropharyngeal abscess in an adult and an elderly woman].	retropharyngeal abscess is reported to be decreasing in frequency in recent years. we report two cases of retropharyngeal abscess that were diagnosed within four years in department of otorhinolaryngology, gifu university hospital. case 1 was a 36-year-old male who was suggested to have an abscess as primary infection and case 2 was a 71-year-old female whose abscess seemed to be a secondary infection following unknown primary infection. endoscopic or open neck drainage as well as antibacterial ...	1998	9545693
subgingival microflora associated with nifedipine-induced gingival overgrowth.	the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ...	1998	9660335
fatal septicaemia in a previously healthy man following a dog bite.	a rare case of fatal septicaemia after a dog bite in a previously healthy individual is presented and discussed. capnocytophaga canimorsus (df-2 bacterium) was isolated from blood cultures. our case shows the importance of immediate antibiotic treatment for all dog and cat bites, regardless of severity.	1998	9684442
[fulminant sepsis by capnocytophaga canimorsus after a dog bite].		1998	9706592
capnocytophaga sepsis in a patient with waldenström's macroglobulinemia.		1998	9709902
risk of animal contact in immunocompromised hosts.		1998	9588438
beware our "best friend": capnocytophaga canimorsus septicaemia after a dog bite.		1998	9612464
[multiorgan failure and peripheral gangrene following a superficial dog bite].	one case of life-threatening disease caused by the gram-negative rod capnocytophaga canimorsus and a similar case without verification are presented. the severe diseases developed two to three days after a superficial dog bite and were characterized by acute development of septicaemia and fulminant disseminated intravascular coagulation syndrome. moreover, the cases were complicated by renal failure, respiratory insufficiency and peripheral gangrene.	1998	9741264
infection of a porous polyethylene orbital implant with capnocytophaga.	a 68-year-old woman experienced an infection of a porous polyethylene orbital implant caused by capnocytophaga after a dental procedure. the infection was unresponsive to both topical and oral antibiotics and required removal of the porous polyethylene orbital implant. capnocytophaga is a capnophilic, gram-negative bacillus. capnocytophaga is a normal commensal of the mouth and is responsible for both gingivitis and periodontal disease. capnocytophaga is a rare cause of ocular infections. this i ...	1998	9842559
[evaluation of a new method for susceptibility testing against fastidious and unusual bacteria].	the susceptibilities of 154 clinical isolates of fastidious and unusual bacteria were determined by the etest, and were compared with the mics for these strains obtained by the agar dilution method. antimicrobial agents; benzylpenicillin, ampicillin, cefotaxime, imipenem, erythromycin, clindamycin and minocycline were used in this study. in general, the etest mics were rapidly and easily interpreted. the etest mics agreed within +/- 1 log2 and +/- 2 log2 dilution, 87.6% and 98.2% respectively in ...	1998	9847518
images in clinical medicine. capnocytophaga canimorsus sepsis.		1998	9854119
purification of immunosuppressive factor from capnocytophaga ochracea.	capnocytophaga, one of the genera of oral bacteria, has been implicated in the pathogenesis of several diseases, including endocarditis, septicaemia and disorders of the oral cavity such as abscesses and periodontal disease. this study examined sonic extracts (se) of capnocytophaga strains for their ability to alter lymphocyte function. the se of tested capnocytophaga caused dose-dependent suppression of spleen cells in response to mitogen. this suppressive effect was heat-labile and sensitive t ...	1998	9856645
postsplenectomy capnocytophaga canimorsus sepsis presenting as an acute abdomen.	patients with intra-abdominal processes that require prompt surgical intervention, including appendicitis, perforated viscus, ischemic bowel, volvulus, and bowel obstruction, often present with signs and symptoms of an acute abdomen. several medical problems can mimic an acute abdomen. overwhelming postsplenectomy infection is a life-threatening condition that can present with acute abdominal symptoms. the incidence of overwhelming postsplenectomy infection ranges from 1% to 25%, and is caused b ...	1998	9865657
the effect of chemotherapy on the supragingival plaque of pediatric cancer patients.	the anaerobic cultivable flora of the dental plaque was investigated in 16 cancer children at days 0, 7, 14 and 21 of a first cure of chemotherapy. results were compared with those obtained in 16 healthy children. diseased children showed more quantitative variations of the flora than the controls, especially during the first week of chemotherapy. whatever the day of sampling, the flora of the diseased children was significantly less complex than that of the controls. viridans streptococci, capn ...	1998	9930358
periodontal status and subgingival microbiota of insulin-dependent juvenile diabetics: a 3-year longitudinal study.	this study examined for 3 years the changes in periodontal status and the possible correlations with selected subgingival microbiota and diabetic conditions in a group of 16 insulin-dependent diabetes mellitus (iddm, jd) patients as compared with their 16 healthy cohabiting siblings (hs). jd patients were monitored every 3 months for levels of glycosylated hemoglobin (hba1c). clinical and microbiological parameters were measured 6 weeks before drawing blood to determine levels of hba1c. periodon ...	1998	9526910
subgingival distribution of periodontopathic bacteria in adult periodontitis and their susceptibility to minocycline-hcl.	the purpose of this study was to investigate the distribution of several periodontopathic bacteria in adult periodontitis, their in vitro susceptibility to minocycline-hcl, and whether the efficacy of the drug changes with a decrease in bacterial susceptibility. twenty-one patients (43 to 75 years old) with 62 periodontal lesions from pockets > or =4 mm participated in the study. after subgingival sampling, an ointment containing 2% minocycline-hcl was applied locally to the selected pockets onc ...	1998	9527568
dog bites: hidden danger of fulminant sepsis.		1998	9542707
supernatant cytotoxicity and proteolytic activity of selected oral bacteria against human gingival fibroblasts in vitro.	the purpose of this study was to determine if endodontic bacterial act in vitro on human gingival fibroblast functions via extracellular products. the bacteria used were prevotella nigrescens, capnocytophaga ochracea, peptostreptoccocus micros and actinobacillus actinomycetemcomitans. supernatants were collected from bacterial cultures at the beginning of the stationary phase when their density was similar. toxins that inhibited fibroblast proliferation were found in all culture supernatants of ...	1998	9569986
beta-sheet antibiotic peptides as potential dental therapeutics.	small, cysteine-rich, beta-sheet peptide antibiotics are found throughout the animalia. though broad spectrum in potential, they may exert selective antimicrobial effects under certain conditions. we have explored the antimicrobial properties of two families of beta-sheet peptide antibiotics, defensins and protegrins, against periodontopathic bacteria. the rabbit defensin np-1 was active against facultative gram-negative bacteria associated with early onset periodontitis, including actinobacillu ...	1998	9573496
subgingival microbiota of shallow periodontal pockets in individuals after head and neck irradiation.	this study aimed at investigating the subgingival plaque microorganisms of shallow pockets (< or = 5 mm) in subjects who previously received irradiation in the head and neck region for treatment of nasopharyngeal carcinoma. direct microscopy and anaerobic culture were used. subgingival paper point samples were taken from 6 tooth-sites (one/sextant) per subject for direct microscopy (n = 108). another set of paper points was taken from the deepest of the previously selected sites (one per subject ...	1998	9573815
identification of bacterial species on or in crevicular epithelial cells from healthy and periodontally diseased patients using dna-dna hybridization.	the purpose of this investigation was to identify bacterial species present on or in crevicular epithelial cells in healthy and diseased sites using dna probes. in order to achieve this aim, further improvements were made in the separation of unattached bacteria from those adherent to epithelial cells isolated from the human gingival crevice or periodontal pocket. then the dna probes were used to determine the prevalence of detectable dna from 15 microbial species on or in crevicular epithelial ...	1998	9573819
characterization of a novel n-acetylneuraminic acid-specific fusobacterium nucleatum pk1594 adhesin.	fusobacterium nucleatum has been identified as significantly associated with sites with active periodontal disease and, as a group, the oral fusobacteria coaggregate with members of all oral bacteria genera tested. monoclonal antibodies were prepared and used in conjunction with other potential inhibitors, such as simple sugars and amino acids, to characterize coaggregation interactions, of f. nucleatum pk1594. four unique monoclonal antibodies, 5h11, 14c7, 19f2 and 29c12, were obtained by their ...	1998	9573822