Publications

hla-d and t lymphocyte reactivity to specific periodontal pathogens in type 1 diabetic periodontitis.	bacterial antigen fragments complexed with class ii major histocompatibility molecules (hla-d) on antigen presenting cells (apcs) stimulate cd4+ t lymphocyte proliferation, presumably to protect the host. this study examined these responses to antigens of two periodontal pathogens in four groups (n = 15) of age- (young adult) and sex-matched caucasian subjects with or without type 1 diabetes and moderate to severe periodontitis: group dp = diabetics with periodontitis; group dnp = diabetics with ...	1993	8277407
a longitudinal microbiological study on osseointegrated titanium implants in partially edentulous patients.	the aim was to longitudinally follow the colonization of putative periodontal pathogens on titanium implants in partially edentulous patients. nineteen individuals, who were partially edentulous and were on the waiting-list to be treated with titanium fixtures, were included in the study. they were treated for periodontal disease with oral hygiene instructions and supra- and subgingival debridement and were left for maintenance. full-mouth recordings of each patient were performed at baseline, i ...	1993	8297958
[cervical abscess caused by capnocytophaga ochracea].	capnocytophaga is a gram-negative capnophilic bacterium which is part of the normal oral flora of humans (c. ochracea, c. gingivalis, c. sputigena) and mammals such as canines, cats, and rodents (c. animorsus and c. cynodegmi). its role in the pathogenesis of periodontal disease is not well defined, and normally it represents an opportunistic germ of low pathogenicity. threatening and fulminant infections have been observed in immunodeficient patients, and lately in immunocompetent hosts. we des ...	1993	8322052
isolation of capnocytophaga species from non-vital anterior teeth.	root-canal infection is very common in india. both aerobic and anaerobic organisms have been claimed for its aetiology. however, isolation of capnocytophaga species from non-vital anterior teeth is sparse. in this study a solitary case of capnocytophagal root canal infection is reported.	1993	8354551
effects of root planing on the distribution of microorganisms adult periodontitis sites.	the aims of this study were to identify the microbial species present in subgingival plaque in patients with adult periodontitis, and to investigate the effects of root planing upon the microbial flora for 12 months following root planing. microorganisms from samples of subgingival plaque obtained from adult periodontitis sites in 15 patients were cultured, both aerobically and anaerobically, on a variety of media and identified. prevotella intermedia and porphyromonas asaccharolytica were the m ...	1993	8148788
dog-associated bacterial infections in humans: isolates submitted to an australian reference laboratory, 1981-1992.	over the period 1981-92, 32 bacterial isolates were referred to the microbiological diagnostic unit from infected dog-bite wounds and 10 isolates were submitted from blood cultures after dog bites or close contact with dogs. the isolates from the bite wounds were identified, or confirmed, as pasteurella multocida (11 isolates), pasteurella dagmatis (3), cdc group m-5 (9), cdc group ef-4a (8) and streptococcus anginosus (1). five of the 9 patients from whom cdc group m-5 was cultured had mixed in ...	1993	8165003
microbial associations in periodontitis sites before and after treatment.	duplicate samples from 110 periodontal sites of 6 mm or more pocket depth in 16 patients were analyzed for the presence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, capnocytophaga spp., campylobacter rectus, eikenella corrodens and fusobacterium nucleatum. the sites were sampled before and after nonsurgical periodontal treatment. no statistically significant associations were found before treatment between any of the analyzed species. after treatment, ...	1993	8247608
microbiology of the pericoronal pouch in mandibular third molar pericoronitis.	the microorganisms associated with mandibular third molar pericoronitis were investigated using direct microscopy and anaerobic culture method. the pericoronal pouch was sampled with paper points in a) 8 patients without mandibular third molar pericoronitis and b) 6 patients with mandibular third molar pericoronitis. under the microscope, the microflora was found to be a complex mixture comprising gram-positive and gram-negative cocci, rods and filaments (including fusiform and curved rods), mot ...	1993	8265205
chorioamnionitis caused by capnocytophaga: case report and review.	we report a case of pathologically demonstrated chorioamnionitis due to capnocytophaga species. the mother had high fever and marked uterine tenderness, and the infant had poor apgar scores, fever, and pulmonary infiltrates. both recovered after receiving specific antibiotics. pathological examination of the placenta revealed acute chorioamnionitis, subchorionitis, and vasculitis of the umbilical vein. the dramatic clinical and pathological features of this case are compared with those of the ni ...	1993	8218689
musculocutaneous mononeuropathy complicating capnocytophaga canimorsus infection.		1993	8232970
[the distribution of normal oral flora in 49 healthy children and juvenile].	the frequencies and proportions of predominant cultiv ableoral bacteria associated with 49 healthy children and juvenile (6-25 yr old) were studied. a total of 72 bacterial species belonging to 28 genus were detected in 195 samples of saliva, fissure plaque, supragingival plaque, and subgingival plaque. the predominant bacteria were oral streptococci, neisseria, actinomyces, capnocytophaga, bacteroides and fusobacterium in the normal oral cavity of healthy children and juvenile. there were diffe ...	1993	8288204
bactericidal effects of povidone-iodine solution to oral pathogenic bacteria in vitro.	seven species of periodontal pathogenic bacteria (bacteroides, gingivalis, bacteroides intermedius, bacteroides melaninogenicus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens) and two control species (streptococcus intermedius, pseudomonas aeruginosa) were selected in order to study the bactericidal effects of 10% povidone-iodine (pvp-i) aqueous solution in vitro. the pvp-i solution was diluted to 10, 20, 50, 100, 200, 400, 800, 1600, 320 ...	1993	8290445
isolation of periodontal species after guided tissue regeneration.	one advanced localized periodontal lesion in each of 10 patients was treated using the guided tissue regeneration procedure. six weeks after placement of expanded polytetrafluoroethylene (eptfe) membranes, microbial samples were taken from each treated site and the membranes were removed. six weeks later the cases were re-evaluated. they had all healed successfully with varying amounts of gain of clinical attachment. gram-negative, anaerobic rods were found in all samples and made up 31% of all ...	1993	8295107
new findings on the microbial flora associated with adult periodontitis.	the predominant microbial flora associated with adult periodontitis was investigated in 21 patients aged 20-40 years. microorganisms from samples of subgingival plaque were cultured on selective and non-selective media under aerobic and anaerobic conditions. ten species or groups out of a total of 31 were found to comprise the predominant cultivable flora: diphtheroids, porphyromonas asaccharolytica, coagulase-negative staphylococci, 'streptococcus milleri'-group, bacteroides ureolyticus, capnoc ...	1993	8315093
antimicrobial action of green tea extract, flavono flavor and copper chlorophyll against oral bacteria.		1993	8375014
simultaneous hybridization and subsequent colour detection of subgingival bacterial dna on colony lifts.	this paper reports the development of a protocol allowing hybridization and detection of dna fixed to nylon colony lifts from up to three species of bacteria simultaneously. half ml samples of serial dilutions of pure cooked-meat broth (cmb) cultures of capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia were grown on trypticase soy blood agar (tsba) plates for 7 days in an anaerobic chamber. from the same cmbs a further set of dilutions was completed that con ...	1993	7507660
[septicemia caused by capnophilic microorganism].		1993	8347707
[endocarditis caused by capnocytophaga spputigena].		1993	8347716
spontaneous ulcerative keratitis in immunocompromised patients.	we studied the occurrence of ulcerative keratitis in five eyes of four patients who were examined at the university of maryland hospital ophthalmology clinic over a 12-month period. all were young women who were intravenous drug abusers, with no known predisposing factors for ulcerative keratitis. two patients had acquired immunodeficiency syndrome (aids), one was human immunodeficiency virus (hiv)-positive, and the fourth refused hiv testing. one had a corneoscleral limbus to corneoscleral limb ...	1993	8430729
microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis.	a series of 993 subgingival microbial samples sent to a diagnostic microbiology laboratory included 196 samples that could be identified as compatible with a clinical diagnosis of refractory or recurrent periodontitis. in descending order of prevalence the associated microbiota included bacteroides forsythus (84%), spirochetes (83%), motile rods (76%), fusobacterium species (68%), porphyromonas gingivalis (63%), campylobacter rectus (47%), capnocytophaga species (38%), prevotella intermedia (23% ...	1993	8463936
the effects of acacia arabica gum on the in vitro growth and protease activities of periodontopathic bacteria.	the antibacterial activity of acacia gum was assessed using fresh isolates and reference strains of actinobacillus actinomycetemcomitans, capnocytophaga spp., porphyromonas gingivalis, prevotella intermedia and treponema denticola. a fine aqueous suspension of gum was produced by sonication and then a soluble fraction isolated by centrifugation and membrane filtration. these preparations were incorporated into columbia agar at doubling concentrations. growth of p. gingivalis and p. intermedia cu ...	1993	8473532
capnocytophaga endophthalmitis.	this article presents three patients with endophthalmitis associated with infection with capnocytophaga species.	1993	8479700
genetic relationship of strains of haemophilus aphrophilus, h. paraphrophilus, and actinobacillus actinomycetemcomitans studied by ribotyping.	strains of h. aphrophilus, h. paraphrophilus, and a. actinomycetemcomitans are phenotypically very similar. ribotyping of 24 strains of h. aphrophilus, 22 strains of h. paraphrophilus, 8 strains of a. actinomycetemcomitans, and one strain each of the species pasteurella aerogenes, h. parahaemolyticus, and the genus capnocytophaga was studied using a non-radioactive digoxigenin labelled probe based on e. coli 16s- and 23s-ribosomal rna. restriction fragments were generated using restriction enzym ...	1993	7690272
the effect of subgingival debridement on periodontal disease parameters and the subgingival microbiota.	the aim of the present investigation was to analyse the effect of subgingival scaling and root planing in subjects who prior to treatment exercised meticulous supragingival plaque control. 300 subjects were examined at baseline and after 1 and 2 years without treatment. after the year 2 examination, 62 subjects were randomly selected for therapy. they were given detailed instruction in proper self-performed toothcleaning measures and were carefully monitored during the subsequent 2 years. follow ...	1993	8501276
an analysis of the subgingival microflora in randomly selected subjects.	the aim of this study was to describe the presence of some microbial species in the subgingival microflora of a randomly selected subject sample. a further objective was to analyze the association between some microbiological characteristics and the periodontal conditions of the subjects. a total of 171 individuals aged 30-65 years were included. a pooled subgingival plaque sample was obtained from the mesial aspect of the 6 ramfjord teeth by the use of the paper-point method. the samples were p ...	1993	8510980
[capnocytophaga spp. bacteremia in compromised patients].	to analyze the clinical characteristics of three patients with capnocytophaga spp. bacteremia.	1993	8512974
capnocytophaga sputigena bacteremia associated with acute leukemia.	during a three-year period, capnocytophaga sputigena bacteremia occurred in three patients with acute leukemia receiving induction therapy on a hematology ward. oral pathology such as periodontitis or severe mucositis was considered to be the most likely source of bacteremia. all three blood culture isolates were identified as that species by deoxyribonucleic acid (dna) homology studies. because of the phenotypical similarity of capnocytophaga species, it is difficult to differentiate them by co ...	1993	8360517
polar lipids of strains of prevotella, bacteroides and capnocytophaga analysed by fast atom bombardment mass spectrometry.	the polar lipid composition of representative strains of bacteroides fragilis, prevotella intermedia, p. melaninogenica and capnocytophaga ochracea was determined. samples were analysed by fab-ms. forty major peaks had m/z values expected for known carboxylate anions, ranging from m/z 211 (tridecenoate) to m/z 381 (pentacontanoate). a further fifty-five major peaks were studied between m/z 561 and m/z 722. forty-five anionic phospholipids of several series were identified. the pearson coefficien ...	1993	8377663
detection of porphyromonas gingivalis in oral plaque samples by use of the polymerase chain reaction.	periodontitis is believed to be caused by bacteria which inhabit periodontal pockets. the identification of these periodontal pathogens by currently available methods requires considerable time and expertise. in this study, we have used a polymerase chain reaction (pcr) assay that is quick, relatively simple, and can detect low numbers of a putative periodontal pathogen. primers specific for the fimbrial gene of porphyromonas gingivalis were selected from the published sequence and used for ampl ...	1993	8388414
capnocytophaga canimorsus bacteraemia demonstrated by a positive blood smear. a case report.	the observation of bacteria in a peripheral blood smear was conducive to the diagnosis of capnocytophaga canimorsus septicaemia in a patient with no definite record of animal bites. multiple rods were seen extracellularly and within the cytoplasm of neutrophils. the blood culture became positive after 18 h of incubation. disseminated intravascular coagulation (dic) was manifest and infarction of the spleen was suspected. direct examination of peripheral blood smears could be a valuable adjunct i ...	1993	8398098
periodontal status of women taking postmenopausal estrogen supplementation.	the association between supplementary estrogen intake and periodontal and gingival status in a total of 228 women 50 to 64 years of age was examined. clinical parameters including visible supragingival plaque, subgingival calculus, probing pocket depth, clinical attachment level, alveolar bone height measurements, and number of remaining teeth were measured. gingival status was recorded as gingival bleeding after gentle manipulation. selected periopathogens, socio-economic, demographic, smoking ...	1993	8277404
periodontal disease and dental caries in relation to primary igg subclass and other humoral immunodeficiencies.	22 females and 3 males with primary hypogammaglobulinaemia (n = 6) or igg subclass deficiencies with (n = 3) or without (n = 16) concomitant iga deficiency were examined for periodontal disease and dental caries. only 1 patient showed more tooth loss than that found in the normal swedish population. 1 patient demonstrated advanced periodontal disease. no patient exhibited more severe dental caries than that of comparable normal swedes. microbiological samples from periodontal pockets and saliva ...	1993	8421120
in vitro antimicrobial activity of the human neutrophil cytosolic s-100 protein complex, calprotectin, against capnocytophaga sputigena.	calprotectin is a complex of two anionic proteins found in abundance in the cytosol of neutrophils, certain macrophages, and oral epithelial keratinocytes. bacteria of the genus capnocytophaga are pathogens of periodontal origin which can cause systemic infection in neutropenic subjects. recently, it has been observed that capnocytophaga may be internalized by neutrophils within the cytosol rather than within a membrane-delimited phagosome. the purpose of this study was to test the in vitro anti ...	1993	8423249
capnocytophaga canimorsus sepsis misdiagnosed as plague--new mexico, 1992.	since 1961, 200 human isolates of capnocytophaga canimorsus--a gram-negative bacterium--have been sent to cdc for identification. infections with this organism may result in a spectrum of manifestations ranging from self-limiting cellulitis to fatal septicemia; most fatal infections have occurred in persons with a history of asplenia, alcoholism, or hematologic malignancy. in most (77%) cases, infection is preceded by a bite or other exposure to dogs. this report summarizes the investigation by ...	1993	8429807
recurrent corneal infections in a patient with the acquired immunodeficiency syndrome.	a 29-year-old female intravenous drug abuser infected with the human immunodeficiency virus suffered recurrent, bilateral corneal infections over an 11-month period. multiple infectious organisms were responsible, including capnocytophaga species, candida albicans, staphylococcus aureus, coagulase-negative staphylococcus, and a-streptococcus. one eye was eviscerated because of corneal perforation and loss of vision; the second eye has maintained good vision. predisposing factors usually associat ...	1993	8500341
septicemia due to capnocytophaga canimorsus after a dog bite in a cirrhotic patient.		1993	8513822
antimicrobial susceptibility of capnocytophaga spp.		1993	8517718
coaggregation between porphyromonas gingivalis and treponema denticola.	to elucidate an ecological profile of several periodontopathogens, the authors examined the coaggregation between cells of porphyromonas gingivalis and oral bacterial strains including treponema denticola in vitro. coaggregation between cells of plaque bacteria was examined by visual assay and phase-contrast microscope. p. gingivalis cells coaggregated with strains of t. denticola and treponema socranskii subspecies socranskii, but did not coaggregate with t. socranskii subspecies buccale, t. so ...	1994	8689730
bacteremia by multidrug-resistant capnocytophaga sputigena.	a case of bacteremia caused by a multiresistant strain of capnocytophaga sputigena in a patient with hematological malignancy is described. the strain presented with a pattern of marked resistance to beta-lactams, with mics of > 256 mg/liter for ampicillin, ticarcillin, piperacillin, cefazolin, and cefuroxime, 64 mg/liter for cefotaxime, and 32 mg/liter for ceftazidime. in addition, the mic of ciprofloxacin was 16 mg/liter. both of these groups of antimicrobial agents are frequently used in the ...	1994	8027314
orogenital contact: a cause of chorioamnionitis?	fusobacterium nucleatum and capnocytophaga species are common oral pathogens and infrequent causes of systemic infection in patients with compromised immunity or disrupted mucosal integrity. the isolation of both organisms from a clinical specimen suggests an oral source of infection.	1994	9205437
identification and analyses of periodontal pathogens in taiwan by microbiological tests.	the purpose of this study was to use microbiological tests for diagnosis of periodontal diseases in taiwan. anaerobic culture, direct microscopy, indirect immunofluorescence (if), and biochemical tests were used to examine 336 samples for the specific microorganisms in subgingival plaque. the results indicated that gram-negative species and motile bacteria were less frequently detected, and in lower proportion, in samples from healthy sites. the bacteria found frequently in healthy group were th ...	1994	9747334
effective method for discriminating between oral bacterial and human alkaline phosphatase activity.	alkaline phosphatase (alpase) activity was quantitatively compared in various kinds of oral bacteria. high alpase activity was detected in 3 species of periodontal bacteria, porphyromonas gingivalis, prevotella intermedia and capnocytophaga sputigena. the alpase activity detected in these bacteria was almost completely inhibited in the presence of 1% sodium dodecyl sulfate (sds). by contrast, the activity of mammalian alpase isoenzymes was not inhibited at all even in the presence of 1% sds. the ...	1994	7478753
capnocytophaga haemolytica sp. nov. and capnocytophaga granulosa sp. nov., from human dental plaque.	two new capnocytophaga species, for which we propose the names capnocytophaga haemolytica and capnocytophaga granulosa, were isolated from supragingival dental plaque of adults. the phenotypic characteristics of these organisms were the same as those of the genus capnocytophaga: gram-negative rods; co2 requirement; gliding motility; catalase negative; oxidase negative; acids produced from d-glucose, d-maltose, d-mannose, and d-sucrose; and acetate and succinate are the major end products of gluc ...	1994	8186098
capnocytophagal pneumonia in a healthy man.		1994	8128719
short-term microbiological and clinical effects of subgingival irrigation with an antimicrobial mouthrinse.	fifty chronic adult periodontitis patients completed a 6-week controlled, double-blind, split mouth clinical study to determine the effects of subgingival irrigation with an antimicrobial mouthrinse on periodontal microflora, supragingival plaque, and gingivitis when used as an adjunct to normal oral hygiene. qualifying subjects had at least four sites, two on each side of the mouth, with probing depths between 4 and 6 mm, which bled on gentle probing. following baseline examinations, subjects r ...	1994	8133413
detection of porphyromonas gingivalis in gingival exudate by a dipeptide-enhanced trypsin-like activity.	porphyromonas gingivalis in subgingival plaque is an important risk factor for future periodontal attachment loss in susceptible adults. the elimination of p. gingivalis is usually concomitant with a healing process. therefore, it should be valuable to have an easy chairside method to follow the effect of periodontal treatment on p. gingivalis detection as well as on its eventual reappearance during the maintenance period. we have previously reported the stimulation of amidolytic activity of p. ...	1994	8133415
modeling the relationship between clinical, microbiologic, and immunologic parameters and alveolar bone levels in an elderly population.	a cross-sectional periodontal study of 74 subjects aged 65 to 75 years was performed. clinical data were collected and related to microbiological and immunological data. a statistical model (step-wise multiple regression) of factors related to bone loss was created initially using clinical data only; then by adding either the microbiologic or immunologic data; and then by using clinical, microbiologic, and immunologic data together. when only clinical data were considered, three factors were fou ...	1994	8133417
ornithobacterium rhinotracheale gen. nov., sp. nov., isolated from the avian respiratory tract.	the phylogenetic position and various genotypic, chemotaxonomic, and classical phenotypic characteristics of 21 gram-negative avian isolates were studied. these strains constitute a genotypically homogeneous taxon in rrna superfamily v, as shown by dna-rrna hybridization data. determination of the 16s rrna sequence of this taxon revealed its detailed position within the "flavobacter" subgroup of the "flavobacter-bacteroides" phylum as described by gherna and woese (r. gherna and c. r. woese, sys ...	1994	8123560
capnocytophaga canimorsus in peripheral blood smears.		1994	8089236
fatal capnocytophaga canimorsus (df-2) septicaemia. a case report.	a 45-year-old man died 2 months after being bitten on the hand by a dog. he developed the rare but characteristic clinical picture of fulminant septicaemia and peripheral gangrene caused by a gram-negative bacillus, capnocytophaga canimorsus, previously known as dysgonic fermenter type 2 (df-2), which is an occasional commensal in the oral flora of dogs and cats. this disease must be anticipated and dog bites appropriately managed to avoid the mortality associated with infection by this micro-or ...	1994	7570239
lipopolysaccharides from various capnocytophaga strains possess potent limulus amoebocyte lysate clotting activity.	lipopolysaccharides (lpss) were extracted by the hot phenol-water method from three atcc strains (c. sputigena atcc 33612, c. ochracea atcc 33596, and c. gingivalis atcc 33624) and three clinical isolates (c. sputigena te-1, c. ochracea ono-26, and c. gingivalis m-12). endospecy was used to determine the limulus amoebocyte lysate clotting activities. the activities of lpss from capnocytophaga strains were stronger than those of escherichia coli, with the exception of the lps from c. gingivalis m ...	1994	7923506
capnocytophaga keratitis. a clinicopathologic study of three patients, including electron microscopic observations.	histopathologic studies of this unusual keratitis caused by capnocytophaga species have not been reported previously.	1994	7997330
the antimicrobial activity of essential oils and essential oil components towards oral bacteria.	a method for reproducibly determining minimal inhibitory concentrations and minimal bactericidal concentrations of plant extracts towards fastidiously and facultatively anaerobic oral bacteria, predicated upon measurements of optical densities in microtitre plate wells, was devised. the antimicrobial properties of some botanical oils were surveyed; of these, australian tea tree oil, peppermint oil, and sage oil proved to be the most potent essential oils, whereas thymol and eugenol were potent e ...	1994	7478759
effect of proteolytic enzymes on the lysis and growth of oral bacteria.	previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. the aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. the effect of trypsin, chymotrypsin and proteinase k on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was test ...	1994	7478762
the tet(q) gene in bacteria isolated from patients with refractory periodontal disease.	twenty-two tetracycline-resistant (tetr) anaerobic and facultative anaerobic bacteria isolated from periodontal pockets of 12 patients with refractory periodontitis were examined for the presence of the tet q determinant by dna-dna hybridization. dot blots of bacterial dna were tested with an intragenic digoxigenin-labelled tet(q) probe consisting of a 1.45 kb ecori/pvuii fragment from plasmid pnfd13-2. southern blots of chromosomal dna digested with the restriction enzyme ecori were also examin ...	1994	7478767
capnocytophaga canimorsus lymphocytic meningitis in an immunocompetent man who was bitten by a dog.		1994	8038330
the most frequently isolated gram-negative anaerobes in saliva and subgingival samples taken from young women.	stimulated saliva and a pooled subgingival sample from the first molars were collected from 30 caucasian women (mean age 30 years) for the presence of gram-negative anaerobic bacteria. the samples were inoculated on nonselective and selective media. the gram-negative anaerobic species occurring in the oral cavity of more than 50% of the subjects were selected for further analysis. fusobacterium nucleatum and prevotella melaninogenica were not recovered from subgingival sites without a simultaneo ...	1994	8008430
the oral gram-negative anaerobic microflora in young children: longitudinal changes from edentulous to dentate mouth.	eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the composition of oral gram-negative anaerobic microflora was investigated in 21 young children (mean age 32 months) with primary dentition. the bacterial findings of samples were compared with those of the same children collected at their edentulous infant period (mean age 3 months). during the primary period, 2 samples were collected from each child: a sample with d ...	1994	7936718
purpura fulminans following a dog bite.		1994	7937460
capnocytophaga bacteremia in neutropenic patients: report of two cases.		1994	7985483
b-cell mitogenicity and il-1 beta production of lipopolysaccharides from various capnocytophaga strains.	to evaluate the etiological roles of capnocytophaga species in the pathogenesis of periodontal disease, we examined the immunological activities of lipopolysaccharides (lpss) from various capnocytophaga strains. all lpss from various capnocytophaga species were mitogenic for balb/c mouse spleen cells, although the responses were lower than those to reference lpss from escherichia coli or salmonella typhimurium. lpss of c. sputigena strains had polyclonal b cell activation and adjuvant activity a ...	1994	7987967
a study of the subgingival microflora of hiv-seropositive and cd4 cell depleted children with hemophilia.	the cultivable subgingival bacterial flora from three hiv-seropositive and cd4 cell depleted children with hemophilia were examined in this study. the numbers of cd4 cells of the subjected children ranged from 4.9 to 16.3 per mm3 blood. streptococcus species, including, beta-hemolytic streptococcus identified as streptococcus mutans and streptococcus sanguis, were predominant in the subgingival plaque samples. actinomyces species were also frequently found. gram-negative rods other than capnocyt ...	1994	7987969
fulminant capnocytophaga canimorsus (df2) septicaemia and diffuse intravascular coagulation in hairy cell leukaemia with splenectomy.		1994	7806888
some microbiological, histopathological and immunohistochemical characteristics of progressive periodontal disease.	the aim of the present investigation was to study the local nature of human periodontal disease by assessing the microbiota and the composition of the tissue lesions at sites with progressive attachment loss in periodontitis susceptible subjects. 300 subjects with periodontal disease were monitored for 2 years without treatment. 8 subjects lost > 2 mm of attachment at > or = 3 sites during both the first and the second 12 month interval. these 8 subjects (progressive disease group; pd) were reca ...	1994	7852619
effect of treatment on some periodontopathogens and their antibody levels in periodontal abscesses.	twenty patients suffering from abscess of periodontal origin were treated and followed clinically and microbiologically for 6 months. microbiological examination was performed by culture technique. one deep and one shallow periodontal pocket in the same patient were examined concomitantly. serum was collected and analyzed for specific antibody level using elisa methodology. treatment included supragingival scaling, drainage, and irrigation of the periodontal pocket with 0.85% sodium chloride and ...	1994	7853125
comparative detection frequency of 6 putative periodontal pathogens in sudanese and norwegian adult periodontitis patients.	twenty-five sudanese and 18 norwegian adult periodontitis patients were selected to participate in this study. the purpose was to compare cultivation results of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, and capnocytophaga species as well as various enteric rods in both populations. in addition, dna probe analysis was used to identify p. gingivalis, p. intermedia, a. actinomycetemcomitans, f. nucleatum, and bacteroides forsythu ...	1994	7853128
microbiota of rapidly progressive periodontitis lesions in association with clinical parameters.	the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ...	1994	7853132
the gingival immune response to periodontal pathogens in juvenile periodontitis.	a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ...	1994	7870467
degradation of immunoglobulin g by periodontal bacteria.	several subgingival microorganisms were tested for their ability to utilize human immunoglobulin g (igg) as a substrate for growth. this was done using a protein-free chemically defined medium, supplemented with igg. stimulation of growth was observed for capnocytophaga ochracea, porphyromonas asaccharolytica, porphyromonas endodontalis, porphyromonas gingivalis, prevotella intermedia, prevotella oralis, lactobacillus catenaforme and streptococcus intermedius. immunoelectrophoresis, sodium dodec ...	1994	7870469
serological cross-reaction between legionella spp. and capnocytophaga ochracea by using latex agglutination test.	cross-reactivity between legionella spp. and capnocytophaga ochracea was noted by latex agglutination tests (serobact legionella; disposable products, adelaide, australia). four of 11 (36%) c. ochracea isolates agglutinated with latex reagents designed to identify legionella pneumophila serogroups. c. ochracea isolated on buffered charcoal yeast extract media may give false-positive results in this legionella latex agglutination assay.	1994	7883900
intracellular multiplication and toxic destruction of cultured macrophages by capnocytophaga canimorsus.	capnocytophaga canimorsus is a gram-negative rod that causes opportunistic infections resulting in bacteremia, septicemia, meningitis, and death in immunocompromised, splenectomized, and alcoholic individuals. infections caused by a related species, capnocytophaga cynodegmi, remain localized at the site of the wound where the organism is introduced. both organisms are part of the normal canine oral flora and are introduced through puncture wounds via dog bites. we found that both c. canimorsus a ...	1995	7642281
an approach to efficacy screening of mouthrinses: studies on a group of french products (i). staining and antimicrobial properties in vitro.	there is a large and increasing number of oral hygiene products available to the general public. as such, it is difficult to demonstrate efficacy for all in long-term home-use studies. the aim of this project was to determine whether screening studies could position the activity and efficacy of a number of antiseptic mouthrinses, available in france, by comparison with an established product. this study reports the experiments in vitro. the products under test were, 4 containing chlorhexidine (e ...	1995	7593703
[aortic endocarditis due to capnocytophaga sputigena].		1995	7603114
clinical, microbiological and host defense parameters associated with a case of localized prepubertal periodontitis.	a 4-year-old japanese boy was referred to osaka university dental hospital because of severe mobility and pain of the right lower primary canine. the canine had severe bone loss and a pocket depth exceeding 5-6 mm. the left lower canine showed slight mobility and moderate alveolar bone loss. the other primary teeth showed no pathogenic findings. the subgingival microflora from the right lower canine was dominated by gram-negative rods, especially capnocytophaga and fusobacterium, while actinomyc ...	1995	7706540
predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis.	the purpose of this investigation was to study the microflora of severe, moderate and minimal periodontal lesions, in young adults with rapidly progressive periodontitis (rpp). subgingival plaque samples were taken from 142 periodontal lesions in 10 young adults aging 25 to 35 years. the examination of the subgingival microflora indicated that certain species, including porphyromonas gingivalis, bacteroides forsythus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, and campylobact ...	1995	7722848
succession of putative peri-implant pathogens after root-form and plate-form implant placement in partially dentate adult monkeys.	this report describes the succession of putative peri-implant pathogens in partially dentate monkeys after dental implantation and prosthetic reconstruction. tooth and implant (6 root-end form, 4 blade-vent implants) sites in eight monkeys were monitored microbiologically and clinically during the pre-implant stage, abutment connection stage, bridge placement stage, and three and six months after the bridge placement stage. tooth and implant sites were cleaned monthly post-extraction. microbiolo ...	1995	7776158
antimicrobial activity of cefotaxime tested against infrequently isolated pathogenic species (unusual pathogens).	the cefotaxime sodium spectrum of activity is very broad and includes many common species and a variety of less frequently isolated pathogens. we have reviewed the clinical microbiology literature (44 references) and the data base of the university of iowa hospitals and clinics (iowa city, ia) to collect data on the activity of cefotaxime against the less common species. cefotaxime was consistently active against actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens, ery ...	1995	7587049
occurrence of certain bacterial species and morphotypes in juvenile periodontitis in chile.	the occurrence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, eikenella corrodens, fusobacterium nucleatum, campylobacter rectus, capnocytophaga species, and certain bacterial morphotypes was determined in 18 affected and 18 unaffected sites in 10 localized juvenile periodontitis (ljp) patients, and in 10 affected and 10 unaffected sites in 5 generalized juvenile periodontitis (gjp) patients. the subgingival proportion of the 7 bacterial species was det ...	1995	7562347
restriction fragment length polymorphism analysis of pcr-amplified 16s ribosomal dna of human capnocytophaga.	the confusion in the taxonomic status of the genus capnocytophaga has made identification of strains and studies on the role of this genus in infectious diseases equivocal. in this study 33 strains of capnocytophaga including reference strains and various clinical isolates, were studied using rflp analysis of 16s ribosomal rna genes. the 16s ribosomal rna (rrna) gene sequences from whole cell suspensions and isolated genomic dna samples were amplified by the polymerase chain reaction (pcr) using ...	1995	7538106
identification of bartonella (rochalimaea) species among fastidious gram-negative bacteria on the basis of the partial sequence of the citrate-synthase gene.	the bacterial genus bartonella (rochalimaea) includes emerging human pathogens with five recognized species. these are fastidious gram-negative bacteria, exhibiting few phenotypic characteristics and whose identification relies upon serotyping, cellular fatty acid analysis, and molecular typing. most of the isolates have been recovered from the blood of patients, and three of the four pathogenic bartonella species are associated with infectious endocarditis. we performed pcr-restriction fragment ...	1995	7545183
microbiological study of juvenile periodontitis in chile.		1995	7548581
some suspected periodontopathogens and serum antibody response in adult long-duration insulin-dependent diabetics.	the subgingival microflora and serum antibody response were examined in long-duration insulin-dependent diabetics and age- and sex-matched non-diabetics. the material consisted of 9 diabetics aged 40-49 years and 19 aged 50-59 years, 13 non-diabetics aged 40-49 years and 21 aged 50-59 years. the bacterial species studied (actinobacillus actinomycetemcomitans, campylobacter rectus, capnocytophaga spp, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia) w ...	1995	7560223
effect of periodontal treatments on serum igg antibody titers against periodontopathic bacteria.	serum igg antibody titers to 7 periodontopathic bacteria in periodontitis patients were measured at the 1st visit and after various periodontal treatments with clinically successful improvement, in order to evaluate what kind of factors are associated with changes of serum antibody titers. 20 patients (10 male and 10 female from 23 to 61 years old) with adult, rapidly progressive periodontitis were enrolled in this study. all patients received initial preparation and most of them also underwent ...	1995	7560233
effect of whole oral bacteria and extracted lipopolysaccharides on peripheral blood leukocyte interleukin-2 receptor expression.	expression of the interleukin-2 receptor (il-2r) on t cells is the molecular mechanism that initiates the g0 to g1 transition and is the critical first step for t cell proliferation in response to antigen. the effect of whole periodontal bacteria and lipopolysaccharides (lps) on peripheral blood mononuclear cell (pbmc) il-2r expression was examined in vitro. lps induced a modest but significant increase in high affinity il-2r alpha/beta (p55/p75 positive) expression on pbmc over untreated cells ...	1995	7562323
comparison of host tissue and bacterial dipeptidyl peptidases in human gingival crevicular fluid by analytical isoelectric focusing.	earlier work has shown that gingival crevicular fluid (gcf) contains dipeptidyl peptidase (dpp) activities that resemble those in host tissue. here, further comparisons were made with enzymes from suspected periodontal pathogens. gingival tissue and gcf were collected from patients with chronic periodontitis. dpp ii and dpp iv fractions with acid and alkaline ph optima, respectively, were separated from crude tissue extracts by gel-filtration chromatography. bacterial cell sonicates were prepare ...	1995	7487574
dog, cat, and human bites: a review.	it is estimated that half of all americans will be bitten by an animal or another human being during their lifetimes. the vast majority of the estimated 2 million annual mammalian bite wounds are minor, and the victims never seek medical attention. nonetheless, bite wounds account for approximately 1% of all emergency department visits and more than $30 million in annual health care costs. infection is the most common bite-associated complication; the relative risk is determined by the species o ...	1995	7490347
[subgingival distribution of periodontopathic bacteria in periodontic patients and susceptibility of these bacteria to minocycline-hcl].	the present study was carried out to examine the distribution of six periodontopathic bacteria in deep periodontal pockets and to reconfirm the effect of periocline on these periodontopathic bacteria. samples from sixty-two periodontal pockets were collected at pocket depths of over 4 mm in twenty-one periodontitis patients aged 43 to 75 years. after sampling, periocline was applied topically to the selected pockets once a week for four weeks and reexamined. the detected rates of the periodontop ...	1995	8583161
properties of alkaline phosphatase in the gingival crevicular fluid.	the isoenzymic properties of the alkaline phosphatase (alp) of the gingival crevicular fluid (gcf) were investigated and compared with those in other cells, such as human polymorphonuclear leukocytes (pmns), and human periodontal ligament cells (pdls), and with those of three species of periodontopathic bacteria: porphyromonas gingivalis 381 (p. gingivalis), prevotella intermedia atcc25611 (p. intermedia), and capnocytophaga sputigena atcc33123 (c. sputigena). the biochemical properties of the i ...	1995	7796488
porphyromonas gingivalis outer membrane vesicles promote bacterial resistance to chlorhexidine.	porphyromonas gingivalis has been frequently associated with some types of periodontal diseases and possesses various mechanisms favoring the pathogenic process. it has been recently observed that vesicles elaborated by p. gingivalis are able to protect bacteria from the bactericidal activity of human serum. the aim of the present investigation was to evaluate the ability of vesicles from p. gingivalis in protecting oral bacteria against chlorhexidine. data indicate that vesicles released by p. ...	1995	8596676
microbial alterations in supragingival dental plaque in response to a triclosan-containing dentifrice.	a total of 325 subjects were entered into a double-blind, stratified 2-treatment clinical study that examined the effects of a dentifrice containing 0.3% triclosan, 2% gantrez copolymer and 0.243% sodium fluoride on supragingival dental plaque and gingivitis. a subset of 159 subjects including 72 men and 87 women participated in the microbiological component of this study, which was designed to detect shifts in supragingival bacterial species in response to triclosan. subjects were divided into ...	1995	8602338
cell envelope and cell wall immunization of macaca fascicularis: effect on the progression of ligature-induced periodontitis.	the nonhuman primate, macaca fascicularis, was used to study the role of immunization with selected members of the periodontopathic microbiota in the longitudinal progression of ligature-induced periodontitis. animals were immunized with cell envelope antigens prepared from porphyromonas gingivalis and prevotella intermedia, and a mixture prepared from fusobacterium nucleatum, campylobacter rectus, and actinomyces viscosus. serum immunoglobulin g (igg), igm and iga isotype antibodies increased s ...	1995	8602339
studies into the microbial spectrum of apical periodontitis.	this study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. a total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. bacteria were identified by applying the following techniques: culturing on various media, gram-staining and using commercially available biochemical test strips. in addition, prevotella intermedia and porphyromonas endodontalis were d ...	1995	8626206
capnocytophaga canimorsus septicemia: fifth report of a cat-associated infection and five other cases.	capnocytophaga canimorsus is a fastidious, slow-growing, gram-negative, rod-shaped bacterium that belongs to the normal oral flora of dogs and cats. human septicemic infections are associated with a high mortality; most cases occur in immunocompromised patients with a history of dog bite. the fifth case of cat-associated septicemia caused by capnocytophaga canimorsus is described. the six case reports presented here point out the characteristics reported previously: (a) cats are a source of huma ...	1995	7588825
chorioamnionitis caused by capnocytophaga: case report.	intraamniotic infection is a significant cause of perinatal morbidity. capnocytophaga, a gram-negative anaerobe found in the oral cavity, has been implicated as an unusual cause of neonatal sepsis. we report a patient in whom this unusual organism was identified and describe the postpartum and neonatal courses.	1995	7631698
periodontopathogens in elderly chinese with different periodontal disease experience.	if an etiological relationship exists between destructive periodontal disease and putative periodontopathogens, they would be expected to have a very low prevalence in periodontally healthy elderly persons. to test this hypothesis, 2 subgroups of elderly, rural chinese (a periodontally "best" and a "worst" group, each comprising 15 persons) were identified in 1990 from a cohort aged 55-69 years, examined in 1984. assessment of changes in periodontal status over the 6-year period were possible by ...	1995	7790524
human root caries: microbiota in plaque covering sound, carious and arrested carious root surfaces.	the plaque microbiota covering sound or carious root surfaces were studied and compared with that covering arrested root caries lesions. from each of these categories five extracted teeth were examined. the experimental design of the study allowed us to relate the qualitative and quantitative microbial composition to the degree of integrity of the root surface. plaque was sampled by a newly developed 'mowing' technique. plaque samples were cultured anaerobically on nonselective columbia blood ag ...	1995	8521441
capnocytophaga (capnocytophaga ochracea group) bacteremia in hematological patients with profound granulocytopenia.	the clinical and microbiological features of 7 cases of bacteremia due to capnocytophaga (capnocytophaga ochracea group) are reported. they were diagnosed during 1991-93 at three hospital clinics. five patients were < 10 years old and all had hematological disorders, 4 acute lymphoblastic leukemia and 1 each had aplastic anemia, non-hodgkin lymphoma, and myelodysplastic syndrome. all were profoundly granulocytopenic with an absolute granulocyte count < 0.13 x 10(9)/l, and all but 1 had oral lesi ...	1995	7660080
clinical and microbiological changes associated with an altered subgingival environment induced by periodontal pocket reduction.	the purpose of the present investigation was to study the effect of an altered subgingival environment, induced by changing the local soft tissue morphology, i.e., pocket depth reduction, on the subgingival microbiota and the clinical conditions. 7 patients aged 30-60 years with generalized marginal periodontitis were selected. patients were instructed in proper oral hygiene and all teeth were cleaned supragingivally. mucoperiosteal flaps were raised and the bone re-contoured to eliminate angula ...	1995	8682925
periodontal status and selected cultivable anaerobic microflora of insulin-dependent juvenile diabetics.	the periodontal status and subgingival microflora of insulin-dependent juvenile diabetic (jd) patients (n = 16, mean age = 11.3) were compared with that of their non-diabetic cohabiting healthy siblings (hs, n = 16, mean age = 13.2). jd patients were monitored every 3 months for levels of glycosylated hemoglobin (hba1c) and clinical and microbial parameters were measured 6 weeks before drawing blood for levels of hba1c (m% = 8.76). clinical indices, measured for the entire permanent dentition, i ...	1995	7562335
peritonitis involving a capnocytophaga sp. in a patient undergoing continuous ambulatory peritoneal dialysis.	capnocytophaga is a genus of gram-negative rods involved mainly in bacteremia in immunocompromised hosts. here we report a case of capnocytophaga peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis. the disease appeared as a superinfection during antimicrobial therapy of a previous episode of peritonitis.	1995	7494050