| [assessment of serum antibodies to suspected periodontal pathogens in periodontitis patients]. | | 1985 | 3939719 |
| capnocytophaga ochracea and group f beta-hemolytic streptococcus suppurative thyroiditis. | the route of infection in acute suppurative thyroiditis is unknown in most cases; when demonstrated, pyriform sinus fistula appears to be the most frequent one. we report the clinical and laboratory findings of a child in whom culture of the thyroid pus yielded two bacteria which are part of the normal oropharyngeal flora: capnocytophaga ochracea and group f beta-hemolytic streptococcus. the preliminary results of the culture, which showed a mixed flora, prompted us to search and to find a pyrif ... | 1986 | 3959182 |
| capnocytophaga species: infections in nonimmunocompromised and immunocompromised hosts. | retrospective review of isolates of capnocytophaga, a genus of capnophilic gram-negative bacilli, referred to the massachusetts state laboratory institute in boston revealed 31 patients with infection due to capnocytophaga, 16 in nonimmunocompromised hosts. these infections included empyema (three patients), lung abscess (one), sinusitis (one), conjunctivitis (three), subphrenic abscess (one), wound (three), osteomyelitis (one), and bacteremia (three). two of the wound infections were closed-fis ... | 1985 | 3965585 |
| sensitivity of capnocytophaga species to bactericidal properties of human serum. | capnocytophaga is a newly described genus of gram-negative bacteria which can cause serious oral and extraoral infections in the susceptible host. in the present study, sensitivity of capnocytophaga spp. to the bactericidal properties of human serum was investigated. laboratory strains representative of capnocytophaga sputigena, c. ochracea, and c. gingivalis and seven oral isolates of capnocytophaga spp. obtained in primary culture were determined to be sensitive to killing by pooled normal ser ... | 1985 | 4044029 |
| isolation of capnocytophaga species with a new selective medium. | a selective medium (cap) composed of a gc agar base supplemented with 1% hemoglobin, 1% polyvitex, and an antibiotic mixture of polymyxin b (15 u/ml), vancomycin (5 micrograms/ml), trimethoprim (2.5 micrograms/ml), and amphotericin b (2.5 micrograms/ml) was compared with another selective medium (tbbp) and two nonselective media--a blood agar and a chocolate agar--to isolate capnocytophaga species from 725 clinical specimens. these included sputa (467 specimens), throat swabs (116 specimens), or ... | 1985 | 4044796 |
| black-pigmented bacteroides species, capnocytophaga species, and actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. | | 1984 | 6583243 |
| capnocytophaga ochracea: an unusual opportunistic pathogen. | | 1985 | 4044877 |
| immunologic profile of juvenile periodontitis. i. lymphocyte blastogenesis and the autologous mixed lymphocyte response. | studies of blastogenesis of lymphocytes in culture from juvenile periodontitis (jp) patients have been inconclusive. experiments demonstrating differences in lymphocyte blastogenesis to preparations of putative periodontopathogens in jp and phytohaemagglutinin (pha) were performed. variables in the blastogenesis assay system recently reported were controlled which included using a range of cell and activator concentrations, incubation times of 3, 5 and 7 days, conical-bottomed microtest wells an ... | 1984 | 6592318 |
| [microbiological aspects of periodontal diseases]. | | 1984 | 6595910 |
| [introduction to immunology: prevention of periodontal diseases]. | | 1984 | 6597216 |
| [activity of serum antibody to periodontopathic bacteria following periodontal treatment]. | | 1984 | 6597230 |
| [bacterial examination in periodontal diseases]. | | 1984 | 6597612 |
| capnocytophaga ochracea pyonephrosis in an infant with obstructive nephropathy. | | 1985 | 4047964 |
| lysozyme-mediated aggregation and lysis of the periodontal microorganism capnocytophaga gingivalis 2010. | the ability of lysozyme to aggregate and lyse the gram-negative capnophilic periodontal microorganism capnocytophaga gingivalis 2010 was monitored optically at 540 nm. both hen egg white and chromatographically purified human lysozymes had significant but similar aggregation potentials for both logarithmic- and stationary-phase bacteria. in general, an increase in enzyme concentration resulted in a graded increase in both the initial and maximum changes in turbidity which occurred during the rea ... | 1985 | 3967924 |
| degradation of human immunoglobulins g and m and complement factors c3 and c5 by black-pigmented bacteroides. | strains of bacteroides, capnocytophaga and fusobacterium were examined by immunological methods for their ability to degrade the human serum proteins igg, igm, c3 and c5. the proteolytic activity of the strains was measured in terms of the breakdown of serum into trichloroacetic acid-soluble material. only black-pigmented bacteroides strains showed proteolytic activity. strains of b. gingivalis degraded igg, igm, c3 and c5, strains of b. intermedius igg and c3, strains of b. endodontalis c3 and ... | 1985 | 3968707 |
| capnocytophaga isolated from the endometrium as a cause of neonatal sepsis. a case report. | capnocytophaga, a genus of gram-negative, anaerobic organisms, was isolated from the endometrium of a postpartum woman and the blood of the neonate. this unusual organism, usually isolated from the oral cavity, is sensitive to most antibiotics except the aminoglycosides. | 1985 | 3973864 |
| incompatibility and host range of pgd10 from capnocytophaga ochraceus, formerly bacteroides ochraceus. | | 1982 | 6281834 |
| unusual sulfonolipids are characteristic of the cytophaga-flexibacter group. | capnocytophaga spp. contain a group of unusual sulfonolipids, called capnoids (w. godchaux iii and e. r. leadbetter, j. bacteriol. 144:592-602, 1980). one of these lipids, capnine, is 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid; the others are, apparently, n-acylated versions of capnine. the lipids were found, in amounts ranging from 2.5 to 16 mumol of capnoid sulfur per g of cells (wet weight), in two cytophaga spp. and also in several closely related organisms: several capnocytophaga ... | 1983 | 6298180 |
| sulfonolipids of gliding bacteria. structure of the n-acylaminosulfonates. | earlier (godchaux, w., and leadbetter, e. r. (1980) j. bacteriol. 144, 592-602; (1983) j. bacteriol. 153, 1238-1246) we demonstrated that an unusual class of sulfonolipids are major components of the cell envelope of gliding bacteria of the genus cytophaga and of closely related genera. one of these lipids, to which we have assigned the trivial name capnine, was purified and was shown to be 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid (which might also be named as 1-deoxy-15-methylhexad ... | 1984 | 6321489 |
| effect of human leukocyte extracts and gingival exudate on periodontopathic bacteria. | the effect of leukocyte hydrolytic enzymes on periodontopathic bacteria was examined in vitro. a frozen and thawed extract of human peripheral blood leukocytes (le) and human gingival crevicular exudate (ge) were shown to be able to cause the release of 50% of the radioactivity from a leukotoxic strain of actinobacillus actinomycetemcomitans (aa y4), labeled by 14c. a nonleukotoxic strain (aa 653) was shown to be more susceptible to both le and ge, up to 68% of the total radioactivity was solubi ... | 1983 | 6345380 |
| ultrastructural studies of the effect of human leukocyte extracts on periodontopathic bacteria. | human gingival crevicular exudate (ge) and leukocyte extracts (le) were previously shown to be able to release the bulk of radioactivity from a variety of 14c-labeled oral bacteria. the present study demonstrates that the release of radiolabel by these enzymatic agents from two periodontopathic bacteria (actinobacillus actinomycetemcomitans strain y4 and capnocytophaga sputigena strain 4) is accompanied by both cell wall and cytoplasmatic damage. the possible role of bacterial products released ... | 1983 | 6345381 |
| septic arthritis involving capnocytophaga ochracea. | septic arthritis of the knee developed in a 21-month-old child. the causative organism, isolated from two separate arthrocenteses, was identified as capnocytophaga ochracea morphologically and by biochemical reactions. previous human infections (bacteremias) have occurred in granulocytopenic hosts with concomitant oral pathology including periodontitis and gingivitis. no abnormalities of oral hygiene were present in this patient, and granulocyte numbers were normal or elevated. eradication of th ... | 1984 | 6715520 |
| [association between specific serum antibodies to subgingival gram-negative bacteria and the degree of periodontal destruction]. | | 1983 | 6355331 |
| the bone-resorbing activities in tissue culture of lipopolysaccharides from the bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis and capnocytophaga ochracea isolated from human mouths. | the activities of lipopolysaccharides (lps) were assessed by measuring the calcium release from mouse calvaria in vitro and compared to that of lps from salmonella typhimurium. stimulation of bone resorption was maximal at an lps concentration of 10 micrograms/ml and at this dose all oral lps preparations showed similar levels of activity and less than that of lps from s. typhimurium. only s. typhimurium lps and b. gingivalis lps retained bone-resorbing activity at 0.1 microgram/ml. no bone-reso ... | 1984 | 6362631 |
| capnocytophaga: a review of the literature. | capnocytophaga species are normal mouth flora but can be opportunistic pathogens causing juvenile peridontitis and bacteremia in the compromised host. indole-negative fusiforms isolated anaerobically or in the presence of increased co2 can presumptively be identified as capnocytophaga species. | 1983 | 6367464 |
| capnocytophaga ochracea infection: two cases and a review of the published work. | bacteria of the genus capnocytophaga are recently recognised pathogens which may cause oral disease and subsequent septicaemia in the immunocompromised host. we present two cases of infection caused by capnocytophaga ochracea; a soft tissue infection in an immunologically normal patient and an episode of septicaemia in a child with leukaemia. the microbiology, pathogenicity, and antimicrobial susceptibility of the genus capnocytophaga are reviewed. | 1984 | 6470184 |
| bacteriology of experimental gingivitis in children. | children are more resistant to gingivitis than are adults. to determine possible differences in their periodontal floras, an experimental gingivitis study, identical in design to one reported earlier with young adults, was conducted with four 4- to 6-year-old children. the incidence of sites that developed gingival index scores of 2 in children was less than one-third of the incidence observed in adults. the composition of the flora of each child was statistically significantly different from th ... | 1984 | 6480100 |
| sodium fluoride susceptibilities of suspected periodontopathic bacteria. | utilizing a blood-based medium, we determined the minimum inhibitory concentrations (mic's) to sodium fluoride (naf) of 45 bacterial strains representing 22 oral species. bacterial susceptibility ranged from 128 micrograms/ml to 2048 micrograms/ml. of those organisms tested, actinobacillus actinomycetemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga ochracea, and actinomyces viscosus were susceptible at lower levels of naf. | 1983 | 6574154 |
| ammonia utilization by a proposed bacterial pathogen in human periodontal disease, capnocytophaga ochracea. | capnocytophaga ochracea strain 25 was originally isolated from a patient with severe juvenile periodontitis. an nad-specific glutamate dehydrogenase (gdh) (ec 1.4.1.2.) was found in cell-free extracts from this organism. the nadh-dependent reductive, or ammonia-assimilating activity (nadh-gdh), of the enzyme was 8-10-fold higher than its nad-dependent oxidative, or ammonia-releasing activity (nad-gdh), suggesting that the primary physiological role of the gdh is ammonia-fixing. capnocytophaga oc ... | 1983 | 6576737 |
| selective recovery of oral capnocytophaga spp. with sheep blood agar containing bacitracin and polymyxin b. | on the basis of in vitro susceptibility testing of antibiotics, dyes, and other antimicrobial agents, we developed and evaluated a medium, tbbp, for the selective isolation of oral capnocytophaga spp. tbbp medium consists of 4% trypticase soy agar (bbl microbiology systems, cockeysville, md.), 5% sheep blood, 0.1% yeast extract, 50 micrograms of bacitracin per ml, and 100 micrograms of polymyxin b per ml. a total of 34 capnocytophaga stock cultures grew well on tbbp medium. except for some strep ... | 1983 | 6833474 |
| pyruvate dehydrogenase activity and metronidazole susceptibility in bacteroides fragilis. | pyruvate dehydrogenase activity was present and comparable (giving a colour change of 202-673 od units/min/g protein in our assay system) in two clinical strains of bacteroides fragilis exhibiting reduced sensitivity to metronidazole (mic 2-8 mg/l at 48 h) in a resistant strain (ingham's) of bact. fragilis (mic 64/mg/l) and in two sensitive strains of bact. fragilis (mic 0.5 mg/l). pyruvate dehydrogenase activity was not detected in a capnocytophaga strain (sensitive to 50 micrograms disc of met ... | 1983 | 6874627 |
| suppression of the periodontopathic microflora in localized juvenile periodontitis by systemic tetracycline. | since recent studies have implicated actinobacillus actinomycetemcomitans in the etiology of localized juvenile periodontitis, this investigation determined the effectiveness of subgingival debridement, topical betadine solution, and systemic tetracycline in suppressing subgingival a. actinomycetemcomitans and other microorganisms. a total of 20 deep periodontal pockets and 10 normal periodontal sites of 6 localized juvenile periodontitis patients was included in the study. each patient was trea ... | 1983 | 6579058 |
| microbiological study of localized juvenile periodontitis in panama. | the occurrence of subgingival actinobacillus actinomycetemcomitans and capnocytophaga in 12 localized juvenile periodontitis and 10 gingivitis patients from panama was determined using selective culture techniques. a actinomycetemcomitans was present in all localized juvenile periodontitis lesions studied and was, on average, recovered in hundred-fold-higher numbers from localized juvenile periodontitis lesions than from gingivitis lesions. capnocytophaga was only recovered in approximately thre ... | 1983 | 6580425 |
| capnocytophaga ochracea septicemia. | a case report describing capnocytophaga ochracea (bacteroides ochraceus) septicemia in a 21-year-old male patient receiving chemotherapy for acute lymphocytic leukemia is presented. the unusual features of this organism are discussed together with a review of the literature. | 1981 | 6939685 |
| [oral gliding bacteria--capnocytophaga]. | | 1981 | 6942071 |
| ultrasonic dispersion of pure cultures of plaque bacteria and plaque. | this study compared the sonic sensitivity of 12 gram-negative and two gram-positive bacteria commonly encountered in plaque associated with periodontal diseases. pure bacterial cultures were grown to standard turbidity, diluted in 1/4 strength prereduced anaerobically sterilized ringer's solution, and aliquots dispersed for 0-180 s, using an mse sonic oscillator at 6 micron under 80% n2, 10% h2 and 10% co2. viable recoveries were determined on anaerobically cultured trypticase soy 5% blood agar ... | 1981 | 6947390 |
| plasmid content of some oral microorganisms isolated from subgingival plaque. | eighty-five strains of bacterial species selected from the predominant cultivable dental plaque flora of patients with different periodontal pathologies were examined for their plasmid content. microorganisms studied included: actinomyces viscosus, a. odontolyticus, bacteroides asaccharolyticus (b. gingivalis), b. melaninogenicus subspecies intermedius, and subspecies melaninogenicus, capnocytophaga ochracea (b. ochraceus), and fusobacterium nucleatum. three b. melaninogenicus isolates showed pl ... | 1982 | 6949951 |
| modulation of in vitro human lymphocyte responses by an exopolysaccharide from capnocytophaga ochracea. | an exopolysaccharide (ep), purified from spent culture fluid previously inoculated with capnocytophaga ochracea strain 25, suppressed in vitro human peripheral blood lymphocyte responses to the mitogen concanavalin a. ep was suppressive when added to cultures before the mitogen, but enhanced responses when added 24 or 48 hr after the mitogen. | 1983 | 6581194 |
| inhibition of neutrophil chemotaxis by soluble bacterial products. | bacterial-neutrophil interactions may be critical determinants of virulence in periodontal diseases. this study was undertaken to examine the ability of major bacterial species from the human oral cavity to inhibit (1) peripheral blood neutrophil chemotaxis, (2) chemotactic formylmethionyl peptide binding, and (3) phagocytosis. included were cultured supernatants and sonic extracts obtained from strains of capnocytophaga, bacteroides gingivalis, fusobacterium nucleatum, bacteroides asaccharolyti ... | 1982 | 6956714 |
| [susceptibility of potential periodontopathic bacteria to metronidazole, spiramycin and their combination]. | a total of 65 bacterial strains originating mostly from subgingival plaque were tested for their susceptibilities to metronidazole, spiramycin, and their combination, ornidazole, erythromycin and tetracycline by means of an agar dilution technique. all agents were active against all anaerobic gram-negative rods. bacteroides gingivalis and fusobacterium nucleatum showed marked susceptibility to metronidazole (mic less than or equal to 0.06 microgram/ml) whereas 4-64 micrograms/ml were required to ... | 1984 | 6584423 |
| polyclonal b-cell activation induced by extracts of gram-negative bacteria isolated from periodontally diseased sites. | the objective of this research was to determine whether gram-negative bacteria frequently isolated from periodontally diseased sites contained polyclonal b-cell activators. polyclonal b-cell activation, which results in nonspecific activation of multiple b-cell clones was analyzed by a hemolysis-in-gel assay designed to detect a broad range of antibody specificities. extracts from numerous bacterial strains, including bacteroides gingivalis, bacteroides melaninogenicus subsp. melaninogenicus, b. ... | 1981 | 6975240 |
| antimicrobial susceptibilities of bacteria associated with periodontal disease. | a total of 193 bacterial strains were tested for their susceptibilities to 14 antimicrobial agents. penicillin g was active at 2 u/ml against 98% of the oral isolates. other antibiotics with good activity were cefoperazone, moxalactam, sch 29,482, and clindamycin. metronidazole was active against more than 90% of the anaerobic bacteria and capnocytophaga but was inactive against most other microaerophilic and facultative strains. | 1983 | 6601929 |
| [endotoxin-like activity in capnocytophaga gingivalis]. | | 1981 | 7035860 |
| degradation of immunoglobulins a2, a2, and g by suspected principal periodontal pathogens. | attention has recently been focused on immunoglobulin a1 (iga1) protease production as a possible virulence factor of bacteria implicated in meningitis and gonorrhea. this report demonstrates that suspected principal etiological agents in destructive periodontal disease include bacteria capable of degrading iga1, iga2, and igg. representative strains of bacteroides melaninogenicus subsp. melaninogenicus and capnocytophaga cleaved iga1 but not iga2 in the hinge region to yield intact fab and fc f ... | 1981 | 7037640 |
| selective medium for isolation of actinobacillus actinomycetemcomitans. | a selective medium, tsbv (tryptic soy-serum-bacitracin-vancomycin) agar, was developed for the isolation of actinobacillus actinomycetemcomitans, tsbv agar contained (per liter) 40 g of tryptic soy agar, 1 g of yeast extract, 100 ml of horse serum. 75 mg of bacitracin, and 5 mg of vancomycin. the tsbv medium suppressed most oral species and permitted significantly higher recovery of a. actinomycetemcomitans than nonselective blood agar medium. the distinct colonial morphology and positive catala ... | 1982 | 7068837 |
| puberty gingivitis in insulin-dependent diabetic children. i. cross-sectional observations. | this cross-sectional study examined the gingivitis occurring at puberty in a population of insulin-dependent juvenile diabetics. seventy-seven children between the ages of 6 and 15 years were examined for gingivitis levels, stages of pubertal maturation and blood levels of glucose and glycosylated hemoglobin. bacterial plaque was sampled from one or more approximal tooth surfaces of every subject and cultured under anaerobic and aerobic conditions on nonselective and selective media. the total c ... | 1983 | 6606030 |
| immunological labeling of oral bacteria after demineralization. an ultrastructural study. | | 1981 | 6118403 |
| effects of endogenous and exogenous inhibitors on the incorporation of labeled precursors into dna by human mononuclear cells. | the degree of responsiveness of lymphoid cells to activation by mitogens and antigens is commonly assessed in vitro by measuring radioactive dna precursor incorporation. several lines of evidence indicate that artifacts affect the results and that these measurements may not be an accurate reflection of cell activation. cultures of blastogenically activated lymphocytes contain soluble, noncytotoxic factors that inhibit the incorporation of radioactive nucleosides into dna by dividing cells withou ... | 1982 | 6128306 |
| aminopeptidase activity of capnocytophaga. | | 1982 | 6219207 |
| capnocytophaga spp. contain sulfonolipids that are novel in procaryotes. | a group of unusual sulfonolipids was found in bacteria of the genus capnocytophaga. one of these lipids, to which we have assigned the trivial name capnine, was isolated in 98% pure form and was identified, by infrared absorption spectrometry, high-resolution mass spectrometry, and other methods, as 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid. another lipid appears to be an n-acylated version of capnine; after acid hydrolysis, its sulfur was recovered in a form chromatographically indi ... | 1980 | 6253439 |
| acid and alkaline phosphatases of capnocytophaga species. i. production and cytological localization of the enzymes. | the two hydrolytic enzymes, acid (acp; ec 3.1.3.2) and alkaline (alp; ec 3.1.3.1) phosphatase, of the three types species of capnocytophaga were examined. both enzymes were produced constitutively, with their activity highest in c. ochracea strain 25. these two degradative enzymes (approximately 10% of the total activity) were released into the growth medium during the latter stages of growth, both as soluble and membrane-bound enzymes. when grown in the presence of high concentrations of organi ... | 1983 | 6661698 |
| acid and alkaline phosphatases of capnocytophaga species. ii. isolation, purification, and characterization of the enzymes from capnocytophaga ochracea. | capnocytophaga ochracea acid (acp; ec 3.1.3.2) and alkaline (alp; ec 3.1.3.1) phosphatase was isolated by ribi cell disruption and purified by sodium dodecyl sulphate - polyacrylamide gel electrophoresis (sds-page.) both phosphatases eluted from sephadex g-150 consistent with molecular weights (migration) of 140 000 and 110 000. sds-page demonstrated a 72 000 and 55 000 subunit molecular migration for acp and alp, respectively. the kinetics of activity of purified acp and alp on p-nitrophenol ph ... | 1983 | 6661699 |
| acid and alkaline phosphatases of capnocytophaga species. iii. the relationship of the enzymes to the cell wall. | acid (acp) and alkaline phosphatase (alp) were localized by physicobiochemical techniques. greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted capnocytophaga species released only 9-28% and 11-43% of the cellular phosphatases, respectively. french pressure cell disruption was more effective in releasing the phosphatases. cell fragments were separated into cell wall, cytoplasmic membrane, and so ... | 1983 | 6661700 |
| cellular fatty acids of capnocytophaga species. | the cellular fatty acid composition of 18 clinical isolates and 4 reference strains of capnocytophaga species (capnocytophaga ochracea, capnocytophaga gingivalis, and capnocytophaga sputigena) was determined by gas-liquid chromatography. the fatty acid profiles of the 22 cultures were essentially identical and were characterized by major amounts (60% or greater) of a saturated, iso-branched-chain, 15-carbon acid (13-methyl-tetradecanoate) and the presence of two relatively uncommon saturated, is ... | 1982 | 7153332 |
| [bacteriological examination of subgingival flora in patients with marginal periodontitis]. | before and 4 weeks after a single course of local treatment, the composition of subgingival microflora of 15 patients with marginal periodontitis was determined by cultural and microscopical examination. a significant reduction in the number of gram-negative microorganisms could be achieved by this treatment. anaerobic sporeless rods like bacteroides and fusobacteria but also spirochetes, eikenella and capnocytophaga were involved in the inflammation. the number of actinomyces in the subgingival ... | 1982 | 7180237 |
| energy metabolism in capnocytophaga ochracea. | among the microflora of the gingival sulcus are members of the genus capnocytophaga which have been implicated as possible etiological agents of juvenile periodontitis and systemic infectious diseases. in this study, the pathway used by c. ochracea strain 25 for generating energy from glucose was investigated. when grown in a complex medium supplemented with glucose and nahco(3), the major end products formed were acetate (4.6 mmol), succinate (11.0 mmol), pyruvate (4.3 mmol), and oxalacetate (3 ... | 1980 | 7216425 |
| [capnocytophaga sputigena in sinusitis]. | the genus capnocytophaga was reported in the literature for the first time in 1979. capnocytophaga are fusiform gram-negative, slowly growing bacteria that require a high pco2. they belong to the normal buccal flora and they are reported as pathogens in infections of the periodontal region with progressive periodontitis and in septicaemia in patients with immunodeficiencies and buccal ulcerations. the present paper is the first to report capnocytophaga sputigena as a pathogen in sinusitis. the p ... | 1983 | 6670527 |
| in vitro susceptibility of capnocytophaga strains to 18 antimicrobial agents. | twenty-seven strains of capnocytophaga were tested for their susceptibility to 18 antimicrobial agents by an agar dilution technique. all strains were susceptible to achievable blood levels of penicillin g, cefaclor, cefoxitin, cefoperazone, moxalactam, clindamycin, chloramphenicol, and tetracycline. most were susceptible to achievable levels of cefamandole, erythromycin, and metronidazole, and more than 10% were resistant to achievable levels of cephalexin and cephradine. with antimicrobial age ... | 1981 | 7283424 |
| disseminated actinomycosis associated with infection by capnocytophaga species. | | 1984 | 6725998 |
| two cases of capnocytophaga bacteremia, one with endocarditis. | | 1984 | 6733613 |
| immunoglobulin g response to subgingival gram-negative bacteria in human subjects. | serum and gingival crevicular fluid from normal healthy adults and patients with periodontitis were screened for immunoglobulin g antibodies to antigens from bacteroides gingivalis 381, bacteroides intermedius 24, bacteroides loescheii atcc 15930, fusobacterium nucleatum atcc 25586, eikenella corrodens 1073, actinobacillus actinomycetemcomitans atcc 29522, and capnocytophaga sp. strain m-12. immunoglobulin g antibody titers to the antigens were measured by an enzyme-linked immunosorbent assay. t ... | 1984 | 6735473 |
| capnocytophaga infection: a risk in the immunocompromised host. | | 1984 | 6741389 |
| api zym system for identification of bacteroides spp., capnocytophaga spp., and spirochetes of oral origin. | a total of 80 oral strains of bacteroides gingivalis, b. asaccharolyticus, b. melaninogenicus subsp. intermedius, b. melaninogenicus subsp. melaninogenicus, capnocytophaga, treponema denticola, and t. vincentii were characterized with the api zym system for 19 enzyme activities. comparison of anaerobic and aerobic incubation with nine reference strains of these organisms showed no important differences. the key differential tests for black-pigmented bacteroides strains and treponemes of oral ori ... | 1982 | 6764781 |
| modulation of immunoreactivity to periodontal disease-associated microorganisms during pregnancy. | the lymphocyte blastogenic response to a panel of antigens and mitogens was assessed in a group of 20 women throughout their pregnancy. in addition, a group of five nonpregnant women was monitored simultaneously to identify variations in response to the same stimulants. the stimulants included orally associated bacterial antigens (streptococcus sanguis, actinomyces viscosus, bacteroides asaccharolyticus, bacteroides melaninogenicus subsp. intermedius, bacteroides [capnocytophaga] ochraceus, and ... | 1980 | 7399691 |
| carbon dioxide metabolism by capnocytophaga ochracea: identification, characterization, and regulation of a phosphoenolpyruvate carboxykinase. | cell suspensions of capnocytophaga ochracea incorporated [14c]nahco3 into a major four-carbon fermentation product, succinate, and cell-free extracts from this organism contained high levels of phosphoenolpyruvate carboxykinase (pepck). pepck is the major, if not the only, co2(hco-3)-fixing enzyme in c. ochracea since cell-free extracts were devoid of pyruvate-dependent and other phosphoenolpyruvate (pep)-dependent co2(hco-3)-fixing enzymes. the reaction products of the enzyme, which was partial ... | 1980 | 6769807 |
| suppression of murine lymphocyte mitogen responses by exopolysaccharide from capnocytophaga ochracea. | an extracellular polysaccharide was purified from culture supernatants of capnocytophaga ochracea 25, a gram-negative bacillus associated with human periodontal disease. the extracellular polysaccharide suppressed in vitro mitogenic responses of murine splenic lymphocytes to concanavalin a and lipopolysaccharide. this suppression wad dose dependent, persisted up to 120 h, and was not caused by direct toxicity of the extracellular polysaccharide. | 1983 | 6822434 |
| [studies of mixed anaerobic infections involving bacteroides gingivalis]. | the infectiousness of several combinations of bacteria containing bacteroides gingivalis and other bacteria associated with periodontal diseases was evaluated by subcutaneous injection to guinea pigs. among the seven mixtures studied, only one permitted the development of an infection easily transmissible to a second guinea pig; this bacterial mixture was composed of b. gingivalis, fusobacterium nucleatum, eubacterium saburreum, and capnocytophaga ochracea (formerly bacteroides ochraceus). owing ... | 1983 | 6883221 |
| the tet(q) gene in bacteria isolated from patients with refractory periodontal disease. | twenty-two tetracycline-resistant (tetr) anaerobic and facultative anaerobic bacteria isolated from periodontal pockets of 12 patients with refractory periodontitis were examined for the presence of the tet q determinant by dna-dna hybridization. dot blots of bacterial dna were tested with an intragenic digoxigenin-labelled tet(q) probe consisting of a 1.45 kb ecori/pvuii fragment from plasmid pnfd13-2. southern blots of chromosomal dna digested with the restriction enzyme ecori were also examin ... | 1994 | 7478767 |
| separation of bacterial ubiquinones by reverse-phase high-pressure liquid chromatography. | a procedure was developed for the separation of ubiquinones by high-pressure liquid chromatography on a reverse-phase c18 column. ubiquinones q6 through q14 were resolved in 20 min and were distinguished from menaquinones by comparing uv spectra at 248 and 275 nm. | 1983 | 6885985 |
| dog, cat, and human bites: a review. | it is estimated that half of all americans will be bitten by an animal or another human being during their lifetimes. the vast majority of the estimated 2 million annual mammalian bite wounds are minor, and the victims never seek medical attention. nonetheless, bite wounds account for approximately 1% of all emergency department visits and more than $30 million in annual health care costs. infection is the most common bite-associated complication; the relative risk is determined by the species o ... | 1995 | 7490347 |
| capnocytophaga found in root canal system of a nonvital tooth. | | 1982 | 6954444 |
| scanning electron-microscopic observations on the in vitro colonization of human dentine by capnocytophaga gingivalis. | | 1982 | 6958369 |
| simultaneous hybridization and subsequent colour detection of subgingival bacterial dna on colony lifts. | this paper reports the development of a protocol allowing hybridization and detection of dna fixed to nylon colony lifts from up to three species of bacteria simultaneously. half ml samples of serial dilutions of pure cooked-meat broth (cmb) cultures of capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia were grown on trypticase soy blood agar (tsba) plates for 7 days in an anaerobic chamber. from the same cmbs a further set of dilutions was completed that con ... | 1993 | 7507660 |
| enzymatic characterization of some oral and nonoral gram-negative bacteria with the api zym system. | the api zym system (analytab products, plainview, n.y.), containing 19 chromogenic substrates, was utilized semiquantitatively to detect extracellular acid and alkaline phosphatases, aminopeptidases, proteases, esterase-lipase, phosphoamidase, and glycosidases in 128 oral and nonoral isolates of black-pigmented bacteroides, actinobacillus, haemophilus aphrophilus, capnocytophaga, fusobacterium nucleatum, wolinella recta, and veillonella parvula. in the black-pigmented bacteroides group of organi ... | 1981 | 7026598 |
| capnocytophaga bacteremia in the compromised host. | capnocytophaga, a co2-requiring gram-negative bacillus, is a recently recognized pathogen in the immunocompromised host. the authors present two cases with granulocytopenia, oral disease and bacteremia with capnocytophage ochracea. the microbiology of this genus, its clinical association with periodontal disease, granulocytopenia and oral ulceration, and its implications for the compromised host are reviewed. | 1982 | 7072641 |
| [endocarditis caused by capnocytophaga ochracea]. | | 1982 | 7079150 |
| immunological activities of capnocytophaga cellular components. | whole cells of a clinical isolate (strain s-3) of the genus capnocytophaga were divided into cell envelope (ce) and cytoplasm (cp) fractions by mechanical disintegration followed by differential centrifugation, and a part of the ce fraction was further fractionated by sodium dodecyl sulfate (sds) treatment into the peptidoglycan and sds-supernatant fractions. the other part of the ce was extracted with butanol-water or hot phenol-water to isolate butanol-lipopolysaccharide and phenol-lipopolysac ... | 1982 | 7095853 |
| virulence factors of capnocytophaga: newer contributions. | | 1982 | 7113984 |
| current taxonomic status of gardnerella vaginalis. | | 1983 | 6607517 |
| relationship between oxygen tension and subgingival bacterial flora in untreated human periodontal pockets. | the predominance of anaerobic bacteria in subgingival plaque samples suggests that the pocket environment is anaerobic. in the present investigation, a small oxygen tension (po2) electrode was inserted into the base of the pocket and the po2 was recorded. in addition, the plaque in these pockets was examined culturally and microscopically. the oxygen tension at the bottom of 36 pockets (5 to 10 mm in depth) ranged from 5 to 27 mmhg (1 mmhg congruent to 133.3 pa) with a mean value of 13.3 mmhg. m ... | 1983 | 6642647 |
| osteomyelitis caused by capnocytophaga ochracea. | a 13-year-old boy is reported with osteomyelitis of the greater trochanter caused by a facultative anaerobic organism (capnocytophaga ochracea) not known to cause infections in normal immunocompetent individuals, nor implicated in bone or joint infections even in compromised hosts. there remains a need to consider unusual organisms and to obtain accurate bacteriologic data in all cases of osteomyelitis. | 1983 | 6655060 |
| cervical abscess due to capnocytophaga ochracea. | a case of a cervical lymph node abscess in a child is reported from which a pure culture of c. ochracea was isolated. this is the first reported case of such an infection due to this organism. the patient, who was not leukopenic and did not receive antibiotics, was cured following excision. | 1982 | 7145731 |
| antimicrobial activity of cefotaxime tested against infrequently isolated pathogenic species (unusual pathogens). | the cefotaxime sodium spectrum of activity is very broad and includes many common species and a variety of less frequently isolated pathogens. we have reviewed the clinical microbiology literature (44 references) and the data base of the university of iowa hospitals and clinics (iowa city, ia) to collect data on the activity of cefotaxime against the less common species. cefotaxime was consistently active against actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens, ery ... | 1995 | 7587049 |
| bacteriology of severe periodontitis in young adult humans. | a total of 78 bacteriological samples were taken from the supragingival tooth surface after superficial cleaning with toothpicks or from the periodontal sulci of 42 affected sites in 21 adolescents or young adults with severe generalized periodontitis. of 190 bacterial species, subspecies, or serotypes detected among 2,723 isolates, 11 species exceeded 1% of the subgingival flora and were most closely associated with the diseased sulci. eleven others were also sufficiently frequent to be suspect ... | 1982 | 7152665 |
| antimicrobial susceptibility of capnocytophaga. | capnocytophaga (bacteroides ochraceus, center for disease control biogroup df-1) is associated with sepsis in granulocytopenic patients and is isolated in large numbers from the affected periodontal pockets in patients with juvenile periodontosis. the minimal inhibitory concentrations (mics) and minimal bactericidal concentrations (mbcs) of 17 antimicrobial agents for 13 strains of capnocytophaga organisms were determined. in addition, the ratio of the mbc to the mic for each antimicrobial agent ... | 1981 | 7247353 |
| [aortic endocarditis due to capnocytophaga sputigena]. | | 1995 | 7603114 |
| [septicemia caused by capnocytophaga sputigena]. | | 1981 | 7290958 |
| chemotaxonomy of the genus capnocytophaga (leadbetter, holt & socransky). | | 1982 | 7118751 |
| [capnocytophaga infections]. | | 1982 | 7122100 |
| biological and chemical characterization of endotoxin from capnocytophaga sputigena. | an endotoxin was isolated from capnocytophaga sputigena strain 4 by a modification of the hot phenol-water method. the extraction procedure yielded a lipopolysaccharide which accounted for approximately 1.5% of the dry weight of the cells. the material was composed of 18.6% lipid (as c(15) fatty acid), 46.5% neutral sugar including 9.6% hexose, 18.3% 6-deoxy sugar, 1.0% 2-keto-3-deoxy sugar, and 4.8% heptose. hexosamine, protein, and phosphorus were found in quantities amounting to 9.0, 2.9, and ... | 1980 | 7358428 |
| detection of a fibroblast proliferation inhibitory factor from capnocytophaga sputigena. | the addition of a sonic extract of capnocytophaga sputigena to the culture fluid to human fibroblasts resulted in an inhibition of cell proliferation. the inhibition was dose-related (200 micrograms/ml caused a 90% inhibition, and 1,000 micrograms/ml caused a complete cessation of growth). the growth inhibition was not due to alterations in culture medium, ph or ionic strength, or to the effects of the c. sputigena lipopolysaccharide. | 1980 | 7358430 |
| septicemia caused by capnocytophaga in a granulocytopenic patient with glossitis. | a patient receiving immunosuppressive treatment for multiple myeloma became granulocytopenic, and acute glossitis developed. blood cultures were positive for capnocytophaga, a fastidious gram-negative bacillus that is known to be part of the normal oral flora and a pathogen for periodontitis. the infection responded to treatment with antibiotics, including penicillin g, to which the organism was sensitive. this is one of the first reports of capnocytophaga septicemia, and suggests that this orga ... | 1980 | 7387286 |
| effective method for discriminating between oral bacterial and human alkaline phosphatase activity. | alkaline phosphatase (alpase) activity was quantitatively compared in various kinds of oral bacteria. high alpase activity was detected in 3 species of periodontal bacteria, porphyromonas gingivalis, prevotella intermedia and capnocytophaga sputigena. the alpase activity detected in these bacteria was almost completely inhibited in the presence of 1% sodium dodecyl sulfate (sds). by contrast, the activity of mammalian alpase isoenzymes was not inhibited at all even in the presence of 1% sds. the ... | 1994 | 7478753 |
| the antimicrobial activity of essential oils and essential oil components towards oral bacteria. | a method for reproducibly determining minimal inhibitory concentrations and minimal bactericidal concentrations of plant extracts towards fastidiously and facultatively anaerobic oral bacteria, predicated upon measurements of optical densities in microtitre plate wells, was devised. the antimicrobial properties of some botanical oils were surveyed; of these, australian tea tree oil, peppermint oil, and sage oil proved to be the most potent essential oils, whereas thymol and eugenol were potent e ... | 1994 | 7478759 |
| effect of proteolytic enzymes on the lysis and growth of oral bacteria. | previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. the aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. the effect of trypsin, chymotrypsin and proteinase k on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was test ... | 1994 | 7478762 |
| comparison of host tissue and bacterial dipeptidyl peptidases in human gingival crevicular fluid by analytical isoelectric focusing. | earlier work has shown that gingival crevicular fluid (gcf) contains dipeptidyl peptidase (dpp) activities that resemble those in host tissue. here, further comparisons were made with enzymes from suspected periodontal pathogens. gingival tissue and gcf were collected from patients with chronic periodontitis. dpp ii and dpp iv fractions with acid and alkaline ph optima, respectively, were separated from crude tissue extracts by gel-filtration chromatography. bacterial cell sonicates were prepare ... | 1995 | 7487574 |
| peritonitis involving a capnocytophaga sp. in a patient undergoing continuous ambulatory peritoneal dialysis. | capnocytophaga is a genus of gram-negative rods involved mainly in bacteremia in immunocompromised hosts. here we report a case of capnocytophaga peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis. the disease appeared as a superinfection during antimicrobial therapy of a previous episode of peritonitis. | 1995 | 7494050 |
| microflora in adult periodontitis. | the subgingival microflora of adult periodontitis was studied in 8 adults (36-47 years) and compared with that of 10 periodontally healthy individuals (36-43 years). a total of 64 periodontal lesions were examined, and classified according to the attachment level in three categories: attachment loss > 6 mm, attachment loss 4-6 mm and attachment loss < 4 mm. also for comparative purposes 20 gingival sulci were evaluated. samples were taken using three standardized paper points and were incubated ... | 1995 | 7494174 |
| restriction fragment length polymorphism analysis of pcr-amplified 16s ribosomal dna of human capnocytophaga. | the confusion in the taxonomic status of the genus capnocytophaga has made identification of strains and studies on the role of this genus in infectious diseases equivocal. in this study 33 strains of capnocytophaga including reference strains and various clinical isolates, were studied using rflp analysis of 16s ribosomal rna genes. the 16s ribosomal rna (rrna) gene sequences from whole cell suspensions and isolated genomic dna samples were amplified by the polymerase chain reaction (pcr) using ... | 1995 | 7538106 |
| identification of bartonella (rochalimaea) species among fastidious gram-negative bacteria on the basis of the partial sequence of the citrate-synthase gene. | the bacterial genus bartonella (rochalimaea) includes emerging human pathogens with five recognized species. these are fastidious gram-negative bacteria, exhibiting few phenotypic characteristics and whose identification relies upon serotyping, cellular fatty acid analysis, and molecular typing. most of the isolates have been recovered from the blood of patients, and three of the four pathogenic bartonella species are associated with infectious endocarditis. we performed pcr-restriction fragment ... | 1995 | 7545183 |