Publications

TitleAbstractYear
Filter
PMID
Filter
the antimicrobial efficacy of commercial dentifrices.this investigation compared the effects of a fluoride dentifrice and toothpastes formulated with antimicrobial ingredients (stannous fluoride and triclosan/copolymer) on oral micro-organisms, including those found in samples taken from the human oral cavity. microbiological techniques determined the minimum inhibitory concentrations (mics) of each dentifrice necessary to inhibit the growth of bacterial strains from the healthy oral cavity, as well as those found in dental caries, periodontal dis ...201020129893
prevalence of capnocytophaga canimorsus and capnocytophaga cynodegmi in dogs and cats determined by using a newly established species-specific pcr.capnocytophagacanimorsus and capnocytophagacynodegmi, fastidious gram-negative rods, are commensal microbes thriving in the oral cavities of dogs and cats. c. canimorsus can sometimes cause fatal systemic infections in humans. in the present study, we established a specific pcr which could identify and distinguish c. canimorsus from c. cynodegmi. the prevalence of capnocytophaga spp. in dogs and cats was determined using this method. c. canimorsus was detected in 74% of dogs and 57% of cats. c. ...201020144514
capnocytophaga canimorsus infections in the netherlands: a nationwide survey.a retrospective nationwide survey on the occurrence of capnocytophaga canimorsus and capnocytopaga cynodegmi infections in the netherlands over 3 years showed 32 cases, of which 31 were caused by c. canimorsus and one by an unspecified oxidase-positive capnocytophaga strain. twenty-eight patients had been diagnosed by blood culture, one by culture from both blood and cerebrospinal fluid (csf), one by culture from a conjunctival swab, and two patients by 16s rrna gene amplification by pcr directl ...201120167010
detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method.to culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. micro-ident and micro-ident plus (hain lifescience gmbh, nehren, germany) tests are two commercially available rapid pcr-based methods for the identification and quantification of putative periodontopathogen bacteria. in this study, we compared these commercial pcr-based hybridization methods with conventional anaerobic culture technique. a total of 36 subgingival plaque sa ...201020219690
characterization of csp-1, a novel extended-spectrum beta-lactamase produced by a clinical isolate of capnocytophaga sputigena.the capnocytophaga sputigena isolate nor, responsible for septicemia, was resistant to amoxicillin and narrow-spectrum cephalosporins. in a cloning experiment, a new gene, bla(csp-1), was identified; this gene encodes a novel extended-spectrum beta-lactamase (esbl) that shares only 52% and 49% identities with the cme-1 and veb-1 beta-lactamases, respectively. the g+c content of this gene, its genetic environment, the absence of conjugation transfer, and its detection in two reference strains sug ...201020308380
the effectiveness of a preprocedural mouthrinse containing cetylpyridinium chloride in reducing bacteria in the dental office.during oral procedures, microorganisms from the oral cavity may contaminate nearby surfaces. the authors evaluated the efficacy of a commercial preprocedural mouthrinse containing 0.05 percent cetylpyridinium chloride (cpc) in reducing the levels and composition of viable bacteria in oral spatter.201020354090
an unusual case of septic shock in a geriatric patient.capnocytophaga canimorsus is a gram-negative rod that is a normal inhabitant of the oral flora of most dogs, cats and other animals. clinically significant infections of humans by this common organism are extremely rare. we present a case of an 87-year-old woman who presented with septic shock and multiorgan system failure. blood cultures were positive for a gram-negative rod that five days after admission was identified as c. canimorsus. she was treated aggressively with intravenous fluid resus ...201020391818
fatal dog bite in the absence of significant trauma: capnocytophaga canimorsus infection and unexpected death.sudden and unexpected nontraumatic death in individuals with asplenia or hyposplenia is usually due to fulminant bacterial sepsis, most often involving streptococcus pneumoniae, neisseria meningitidis, and hemophilus influenzae. we report a case of a previously well 40-year-old man who died 5 hours after hospital admission. at autopsy waterhouse-friderichsen syndrome was identified and capnocytophaga canimorsus was subsequently isolated on antemortem blood cultures. infection of humans with this ...201020407357
subtle bacterial endocarditis due to kingella kingae in an infant: a case report.a 9-month-old infant presented with fever, dyspnoea, and a murmur. echocardiography showed a mitral vegetation with significant regurgitation. mitral valve plasty was performed on day 6, and was polymerase chain reaction positive for kingella kingae. the cardiac outcome was favourable. this case illustrates a subtle presentation of k. kingae mitral valve infective endocarditis in a normal-cardaic infant, treated with early surgery, and the agent belonged to the hacek (haemophilus spp actinobacil ...201020465860
mechanical non-surgical treatment of peri-implantitis: a single-blinded randomized longitudinal clinical study. ii. microbiological results.peri-implantitis is common in patients with dental implants. we performed a single-blinded longitudinal randomized study to assess the effects of mechanical debridement on the peri-implant microbiota in peri-implantitis lesions.201020507380
[usefulness and limit of gram staining smear examination].gram staining is one of the most simple and inexpensive methods for the rapid diagnosis of bacterial and fungal infections. it yields results much faster than culture, and provides important data for the patient's treatment and prognosis. however, a difference exists in the quality and quantity of information yielded by gram staining smears based on the experience and knowledge of those conducting the tests. therefore, a risk of misdiagnosis based on the information obtained from gram staining s ...201020560458
postsplenectomy infection - strategies for prevention in general practice.the spleen plays a crucial role in human defence against infection. patients who are asplenic or hyposplenic are at increased risk of severe sepsis due to specific organisms. overwhelming postsplenectomy infection (opsi) has a mortality rate of up to 50%.201020628676
capnocytophaga sputigena primary iliopsoas abscess.iliopsoas abscess is usually secondary to the spread of infection from a contiguous focus. primary disease is uncommon, except in children where staphylococcus aureus is the main pathogen. we report a 60-year-old woman who developed a primary iliopsoas abscess as a result of haematogenous spread of capnocytophaga sputigena from a palatal fistula and chronic sinusitis due to previous treatment for nasopharyngeal carcinoma. pyomyositis due to unusual and fastidious gram-negative bacilli should be ...201020634330
subgingival microbial profiles of smokers with periodontitis.the subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. we tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16s sequencing. smokers ...201020739702
[permanent hearing loss following dog bite. capnocytophaga canimorsus caused severe infection with sepsis]. 201020812562
bacterial 16s sequence analysis of severe caries in young permanent teeth.previous studies have confirmed the association of the acid producers streptococcus mutans and lactobacillus spp. with childhood caries, but they also suggested these microorganisms are not sufficient to explain all cases of caries. in addition, health-associated bacterial community profiles are not well understood, including the importance of base production and acid catabolism in ph homeostasis. the bacterial community composition in health and in severe caries of the young permanent dentition ...201020826648
identification of bacteria associated with feline chronic gingivostomatitis using culture-dependent and culture-independent methods.feline chronic gingivostomatitis (fcgs) is a chronic inflammatory disease of the oral cavity that causes severe pain and distress. there are currently no specific treatment methods available and little is known regarding its aetiology, although bacteria are thought to play a major role. the purpose of this study was to identify the oral bacterial flora in normal and diseased cats. oral swabs were obtained from the palatoglossal folds of eight cats (three normal and five fcgs) and were subjected ...201120828946
capnocytophaga canimorsus sepsis. 201020836255
il6 -174 genotype associated with aggregatibacter actinomycetemcomitans in indians.genetic factors have recently been associated with presence of aggregatibacter actinomycetemcomitans subgingivally in populations living in industrialized countries. the aim of this study was to analyse associations between interleukin-6 (il6) single nucleotide polymorphisms and presence and levels of a. actinomycetemcomitans and other subgingival microbes in a rural indian population.201120860759
clonal analysis of the microbiota of severe early childhood caries.severe early childhood caries is a microbial infection that severely compromises the dentition of young children. the aim of this study was to characterize the microbiota of severe early childhood caries.201020861633
amplification of minute amounts of oral bacterial dna for real-time quantitative pcr analysis.high-throughput technologies for typing caries or health-associated bacterial populations including pcr, dna microarrays and next-generation sequencing techniques require significant amounts of bacterial dna. in clinical settings, the amount of sampled dna is often limited and amplification is therefore essential. protocols should be able to reproducibly amplify sequences in order to maintain initial sequence ratios and should not bias the representation of particular dna sequence types.201020881394
investigation of supragingival plaque microbiota in different caries status of chinese preschool children by denaturing gradient gel electrophoresis.this study aimed to detect differences in the richness of total supragingival plaque microbiota as well as the species composition of oral streptococci involved in the different stages of dental caries. forty-five plaque samples were collected from caries-moderate (cm, 4 ≤ dmfs ≤ 6), caries-susceptible (cs, dmfs ≥ 10), and age-matched caries-free children separately. total dna was isolated directly from each sample, and polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) ...201020927511
[fatal sepsis due to capnocytophaga canimorsus after dog bite].a 56-year-old man was bitten by a dog. 8 days later he was admitted to a hospital because of severe deterioration of his clinical situation. he developed the clinical picture of fulminant sepsis. in spite of aggressive intensive care therapy the patient died 24 hours later. capnocytophaga canimorsus was identified from initial blood cultures. this gram-negative rod is part of the normal oral flora of dogs and cats. it can be spread to humans by bites, licking or scratches and causes severe infec ...201020981594
prioritizing emerging zoonoses in the netherlands.to support the development of early warning and surveillance systems of emerging zoonoses, we present a general method to prioritize pathogens using a quantitative, stochastic multi-criteria model, parameterized for the netherlands.201021085625
"beware man's best friend".case report.201021102283
playing cat and mouse with a gram-negative organism causing peritonitis. 201021148063
killing of anaerobic pathogens by predatory bacteria.recently, the predation of bdellovibrio bacteriovorus on a periodontal pathogen has been described. the current study explores the potential antimicrobial activity of a range of predatory bacteria against key periodontal pathogens. a number of representatives from the bdellovibrio, bacteriovorax and peredibacter lineages (called 'balos') were tested for their activity towards a group of key periodontal pathogens and an optimal multiplicity of infection was established. as the oral cavity contain ...201021214872
presence of the oral bacterium capnocytophaga canimorsus in the tooth plaque of canines.capnocytophaga canimorsus is a potentially pathogenic microorganism when transmitted to humans from the oral cavity of canines. although there is some knowledge about the frequency of occurrence in canines, it is uncertain whether there is a correlation between its occurrence and lifestyle, health, or breed of dog. samples of tooth plaque from a total of 131 canines were collected, cultured on selective media, and tested using physiological and molecular analyses to help discern the presence of ...201021239122
capnocytophaga canimorsis a cause of septicaemia following a dog bite: a case review.described in this study is the case of a 53-year-old woman who developed a life-threatening infection caused by the bacterium capnocytophaga canimorsus (c. canimorsus), subsequent to being bitten by a dog. the patient presented to an emergency department with a 24-h history of diarrhoea and vomiting with dehydration but within 36 h of presentation developed an overwhelming severe sepsis with septic shock, disseminated intravascular coagulation, acute renal failure, metabolic acidosis and threate ...201121242095
demographic study of expulsive hemorrhages in 3 patients with infectious keratitis.to investigate the demography, causative bacteria, and clinical findings in 3 patients with expulsive hemorrhage.201121242778
complete genome sequence of capnocytophaga ochracea type strain (vpi 2845).capnocytophaga ochracea (prévot et al. 1956) leadbetter et al. 1982 is the type species of the genus capnocytophaga. it is of interest because of its location in the flavobacteriaceae, a genomically not yet charted family within the order flavobacteriales. the species grows as fusiform to rod shaped cells which tend to form clumps and are able to move by gliding. c. ochracea is known as a capnophilic (co(2)-requiring) organism with the ability to grow under anaerobic as well as aerobic condition ...200921304645
granulomatous duodenitis mimicking crohn's disease caused by capnocytophaga sp. 201121351203
use of 16s ribosomal rna gene analyses to characterize the bacterial signature associated with poor oral health in west virginia.west virginia has the worst oral health in the united states, but the reasons for this are unclear. this pilot study explored the etiology of this disparity using culture-independent analyses to identify bacterial species associated with oral disease.201121362199
antibiotic susceptibility of cocultures in polymicrobial infections such as peri-implantitis or periodontitis: an in vitro model.background: although polymicrobial infections, such as peri-implantitis or periodontitis, were postulated in the literature to be caused by synergistic effects of bacteria, these effects remain unclear looking at antibiotic susceptibility. the aim of this study is to compare the antibiotic susceptibilities of pure cultures and definite cocultures. methods: laboratory strains of aggregatibacter actinomycetemcomitans (aa) (previously actinobacillus actinomycetemcomitans), capnocytophaga ochracea ( ...201121405933
clustering of subgingival microbial species in adolescents with periodontitis.it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ...201121410554
facial cellulitis because of aggregatibacter (actinobacillus) actinomycetemcomitans and capnocytophaga species in an immunocompetent patient.the species of capnocytophaga and aggregatibacter are normal flora and mostly cause periodontal diseases. the soft tissue infection caused by aggregatibacter often is associated with actinomyces species. beside, most capnocytophaga infections are described in immunocompromised patients. we identified facial cellulitis caused by capnocytophaga spp and aggregatibacter (actinobacillus) actinomycetemcomitans in a 16-year-old immunocompetent female.201121439519
bacterial diversity of periodontal and implant-related sites detected by the dna checkerboard method.the aim of this study was to compare the microbial composition of the subgingival biofilm from teeth and implant sulci in relation to contents originating from internal parts of the implant, abutment and implant prosthesis. twenty subgingival biofilm samples from the mesial and distal aspects of each tooth/implant and 29 samples from the internal parts of titanium implants, abutments and implant prostheses were evaluated for the presence of 18 bacterial species using dna checkerboard and the dif ...201121533621
the role of bacteria in oral cancer.despite the widening interest in the possible association between bacteria and different stages of cancer development, our knowledge in its relation to oral cancers remains inadequate. the aim of this review article is to derive a better understanding on the role of various micro-organisms in the etiogenesis of oral cancers through all the available data on the pubmed. different bacteria have been proposed to induce carcinogenesis either through induction of chronic inflammation or by interferen ...201021584217
septicemia due to capnocytophaga canimorsus following dog bite in an elderly male.a previously healthy, 67-year-old, man with past medical history of myocardial infarction and hypertension was rushed to the emergency room after sudden onset of fever, chills, severe rigors, hypotension, tachycardia and vomiting. the patient was diagnosed as being in septic shock, and investigations revealed intracellular gram-negative bacilli in polymorphonuclear leukocytes in the peripheral smear. a history of dog contact was elicited after this very unusual and rare finding. cultures confirm ...201121623093
experimental alveolitis in rats: microbiological, acute phase response and histometric characterization of delayed alveolar healing.the pathogenesis of alveolitis is not well known and therefore experimental situations that mimic some features of this disease should be developed.201121625744
effect of green tea catechin, a local drug delivery system as an adjunct to scaling and root planing in chronic periodontitis patients: a clinicomicrobiological study.evaluate the adjunctive use of locally delivered green tea catechin with scaling and root planing, as compared to scaling and root planing alone in the management of chronic periodontitis.201121772720
the genome and surface proteome of capnocytophaga canimorsus reveal a key role of glycan foraging systems in host glycoproteins deglycosylation.capnocytophaga canimorsus are commensal gram-negative bacteria from dog's mouth that cause rare but dramatic septicaemia in humans. c. canimorsus have the unusual property to feed on cultured mammalian cells, including phagocytes, by harvesting the glycan moiety of cellular glycoproteins. to understand the mechanism behind this unusual property, the genome of strain cc5 was sequenced and analysed. in addition, cc5 bacteria were cultivated onto hek 293 cells and the surface proteome was determine ...201121762219
in vitro evaluation of bacterial leakage along the implant-abutment interface of an external-hex implant after saliva incubation.the aim of this in vitro study was to evaluate bacterial leakage along the implant-abutment interface under unloaded conditions. twelve premachined abutments with plastic sleeves and 12 dental implants were used in this study. prior to tests of bacterial leakage, samples from the inner parts of the implants were collected with sterile microbrushes to serve as negative controls for contamination. after casting, the abutments were tightened to 32 ncm on the implants. the assemblies were immersed i ...201121841988
the n-glycan glycoprotein deglycosylation complex (gpd) from capnocytophaga canimorsus deglycosylates human igg.c. canimorsus 5 has the capacity to grow at the expenses of glycan moieties from host cells n-glycoproteins. here, we show that c. canimorsus 5 also has the capacity to deglycosylate human igg and we analyze the deglycosylation mechanism. we show that deglycosylation is achieved by a large complex spanning the outer membrane and consisting of the gpd proteins and sialidase siac. gpdd, -g, -e and -f are surface-exposed outer membrane lipoproteins. gpddef could contribute to the binding of glycopr ...201121738475
capnocytophaga canimorsus septicaemia in an asplenic patient with systemic lupus erythematosus.asplenic patients are highly susceptible to infections with encapsulated bacteria such as capnocytophaga canimorsus, a gram negative bacillus which is a commensal organism in cat and dog saliva. we describe the first case of septic shock due to c canimorsus infection occurring in a 51-year-old woman with functional asplenism related to systemic lupus erythematosus with antiphospholipid antibodies. because of the potential severity of this infection, physicians have to be aware of its occurrence ...200921747901
capnocytophagia canimorsus sepsis in an asplenic heart transplant candidate with a left ventricular assist system.fulminant septic shock associated with capnocytophagia canimorsus bacteremia developed in a 54-year-old asplenic man, a heart transplantation candidate who was supported with a heartmate ii left ventricular assist system, after he experienced a dog bite. he improved after administration of broad-spectrum antibiotics, which were narrowed to ampicillin/sulbactam for a prolonged 6-week course, and subsequently recovered fully.201121736240
Early microbial succession in redeveloping dental biofilms in periodontal health and disease.Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ...201121895662
Imtechella halotolerans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from estuarine water.A novel Gram-negative, rod-shaped, non-motile, non-sporulating bacterium, designated strain K1T, was isolated from estuarine water sample collected from Kochi, Kerala, India. Colonies on marine agar were circular, 2-2.5 mm in diameter, shiny, yellow, translucent and convex with entire margins. Negative for ornithine decarboxylase, lysine decarboxylase, nitrate reduction, H2S production. The fatty acids were dominated by branched with iso- fatty acids with a high abundance of iso-C15 : 0, iso-C15 ...201122199208
profiling of dental plaque microflora on root caries lesions and the protein-denaturing activity of these bacteria.to profile plaque microflora on root-caries lesions, and to examine the protein-denaturing activity as a pilot study.201122165457
[The presence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in companion animals in the Netherlands].The presence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in companion animals in The Netherlands. This article reports about a research that is done to determine the presence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in companion animals in the Netherlands using a species specific PCR in combination with classic microbial isolation. Because of the high presence of the bacteria in the mouth of especially cats and dogs (respectively 21 and 19%) and the severe conseque ...201121870573
the subgingival microbiota of papillon-lefèvre syndrome.background: there is little information about the microbiological profiles of periodontal lesions in papillon-lefèvre syndrome (pls) and the significance of bacteria in the pathogenesis of periodontitis in these patients. this comprehensive analysis of the subgingival microbiota in pls patients used 16s rrna clonal analysis and the 16s rrna-based human oral microbe identification microarray (homim). methods: thirteen pls patients, from 7 unrelated families, volunteered for this microbiological s ...201122141356
evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals.the aim of this study was to evaluate the composition of the subgingival microbiota of alcoholic and non-alcoholic individuals.201121864643
Capnocytophaga lung abscess in a patient with metastatic neuroendocrine tumor.Capnocytophaga species are known commensals of the oral cavity of humans and animals (mainly dogs and cats) and are a rare cause of respiratory tract infections. We report a case of cavitary lung abscess caused by a Capnocytophaga species in a patient with a metastatic neuroendocrine tumor.201222075586
Complete genome sequence of the dog commensal and human pathogen Capnocytophaga canimorsus strain 5.Capnocytophaga canimorsus is a commensal Gram-negative bacterium, originally isolated from a dog's mouth, that causes septicemia in humans. C. canimorsus has the unusual ability to feed on host cells, including phagocytes. This capacity depends on surface-exposed glycan-foraging systems. Here we present the first complete genome sequence of a C. canimorsus strain (Cc5).201121914877
investigation of six selected bacterial species in endo-periodontal lesions.didilescu ac, rusu d, anghel a, nica l, iliescu a, greabu m, bancescu g, stratul si. investigation of six selected bacterial species in endo-periodontal lesions. international endodontic journal, 45, 282-293, 2012. abstract: aim  to investigate and determine possible associations of six tested bacteria belonging to 'orange' and 'green' complexes, in endo-periodontal lesions: parvimonas micra, fusobacterium nucleatum, campylobacter rectus, eubacterium nodatum, eikenella corrodens and capnocytopha ...201122077868
bacterial leakage along the implant-abutment interface: culture and dna checkerboard hybridization analyses.objective: bacterial species have been found harboring the internal surface of dental implants as consequence of their failed connections. the aim of the present study was to compare the detection frequency of bacterial leakage from human saliva through the implant-abutment interface, under non-loading conditions, using either dna checkerboard or culture method. materials and methods: thirty dental implants with hexagonal platforms were connected to pre-machined abutments according to the manufa ...201122092990
Microbiology of facial cellulitis related to dental infection.The study objective was to identify bacteria responsible for facial cellulitis of dental origin.201121907512
'Biting the hand that feeds': fever and altered sensorium following a dog bite.Capnocytophaga canimorsus infection is the most severe and rapidly progressive bacterial infection transmitted by dog bite and fortunately is very rare. The authors describe a 68-year-old gentleman who presented in an acute confusional state 2 days after having been bitten on the left hand by a dog. Despite immediate broad-spectrum intravenous antibiotics, he developed significant sequelae including disseminated intravascular coagulopathy, microvascular emboli leading to peripheral necrosis, wid ...201122101595
capnocytophaga canimorsus endocarditis with root abscess in a patient with a bicuspid aortic valve. 200921977282
molecular detection of human periodontal pathogens in oral swab specimens from dogs in japan.periodontal diseases are known to be major diseases in humans, and are also common in dogs. the purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. the distribution of an animal gingival organism porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including porphyromonas gingivalis, treponema denticola, tannerella forsythia, capnocytophaga ochracea, capnocyt ...201121916371
synergistic effect on biofilm formation between fusobacterium nucleatum and capnocytophaga ochracea.the formation of dental plaque biofilm by specific gram-negative rods and spirochetes plays an important role in the development of periodontal disease. the aim of this study was to characterize biofilm formation by fusobacterium nucleatum and capnocytophaga ochracea. coaggregation between f. nucleatum and capnocytophaga species was determined by visual assay. biofilm formation was assessed by crystal violet staining. enhancement of biofilm formation by f. nucleatum via soluble factor of c. ochr ...201222252100
Displaying items 901 - 961 of 961