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microbiological and clinical effects of chlorhexidine digluconate and hydrogen peroxide mouthrinses on developing plaque and gingivitis.while the ability of chlorhexidine (chx) to prevent plaque formation and inhibit the development of gingivitis has been well documented in the literature, the therapeutic value of hydrogen peroxide (h2o2) in preventing gingivitis is in dispute. the purpose of this study was to compare the clinical and microbiological effects of an established therapeutic agent, such as chlorhexidine with that of h2o2 in the experimental gingivitis model. following a period of stringent oral hygiene, 32 subjects ...19883422246
induction of activated lymphocyte killing by bacteria associated with periodontal disease.complex interactions occur among host defense cells during bacterial infection. bacteria and bacterial products may enhance or inhibit the effector and regulatory activity of human lymphocytes. accordingly, we tested the ability of human periodontal pathogens to activate peripheral blood lymphocytes using standard chromium-release assays to measure lymphocyte-mediated cytolysis. human adherent-cell depleted peripheral blood lymphocytes (pbl) with the addition of glutaraldehyde-fixed bacteria at ...19883130415
suspected periodontopathic bacteria associated with broken-mouth periodontitis in sheep. 19882963900
antimicrobial agents in the treatment of periodontal diseases: special aspects on tetracycline and doxycycline.increasing evidence for the involvement of specific microorganisms in the etiology of human periodontal diseases has appeared during the last few years. the microorganisms most likely to cause periodontal disease are actinobacillus actinomycetemcomitans, bacteroides gingivalis, bacteroides intermedius and capnocytophaga species. many of the strains are resistant to tetracycline and doxycycline. recently penicillin-resistant b. gingivalis and b. intermedius have also been described. a. actinomyce ...19883047856
effect of clindamycin on neutrophil killing of gram-negative periodontal bacteria.periodontal diseases are infections of the tissues supporting the dentition. recognition that relatively specific microfloras are associated with distinct clinical forms of periodontal disease has prompted the use of antimicrobial agents as adjuncts in periodontal therapy. clindamycin is one of several antibiotics known to concentrate in bioactive form in neutrophils and to potentiate phagocyte bactericidal activity against certain bacteria. neutrophils appear to play a key role in host defense ...19883056238
[initial antibacterial prophylaxis after bone marrow allograft. pilot study with systemic vancomycin].in 22 consecutive patients treated by allogenic bone marrow transplantation, the authors report their experience in complete gastrointestinal decontamination and prophylactic systemic vancomycin. neither septicemia from the low digestive tract nor with gram positive is noticed. a child developed septicemia with capnocytophaga ochracea, resistant to vancomycin. there is no infectious death in this study and no significative toxicity is reported.19883059276
[local immune responses to periodontopathic bacteria. i. relationship of the igg titers in serum and gingival crevicular fluid]. 19883060555
periodontopathic micro-organisms in the rice rat (oryzomys palustris).the rice rat spontaneously develops periodontal disease, and this process can be accelerated if the animal is placed on a high sucrose diet. as the aetiological agent(s) is unknown, this study was undertaken to determine the micro-organisms associated with bone loss. animals at weaning were placed on either a high sucrose diet or on regular rat chow and were sampled periodically for a variety of micro-organisms. bacteroides spp., spirochetes, and actinobacillus actinomycetemcomitans were not iso ...19883060702
degradation of basement membrane collagen by proteases from some anaerobic oral micro-organisms. 19883077815
identification of periodontopathic bacteria based upon their peptidase activities. 19883078100
ocular capnocytophaga infection in an edentulous, immunocompetent host.severe microbial keratitis developed in a 74-year-old women receiving long-term corticosteroids. corneal smears revealed slender, fusiform gram-negative bacilli, but the lesion continued to worsen despite intensive antibiotic treatment. after several days, capnocytophaga sputigena was identified on culture. specific antimicrobial therapy was instituted, supplemented with corneal cryotherapy. there was gradual clinical improvement. however, a sterile corneal melt led to the late loss of the eye. ...19883168490
peripartum infections with capnocytophaga. a case report.capnocytophaga is an increasingly recognized genus of pathogens. originally associated with periodontal disease, this fastidious, gram-negative organism is now established as the cause of a wide variety of infections in both the immunocompetent and immunocompromised. we treated a woman for amnionitis and subsequent neonatal colonization. capnocytophaga should be considered one of the various organisms that can complicate the peripartum period.19883172065
the effects of periodontal therapy on serum antibody (igg) levels to plaque microorganisms.the influence of periodontal therapy on serum antibody titers to selected periodontal disease-associated microorganisms was assessed in 23 patients having chronic inflammatory periodontal disease (cipd). the immunoglobulin g (igg) titers were determined by the microelisa technique in serum samples obtained prior to treatment; following a hygienic phase which included scaling, root planing, and oral hygiene instruction; following surgical treatment; and one year and two years following hygienic p ...19883198782
the comparative cytotoxicity of periodontal bacteria.the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ...19883204481
the effect of human bile on bacteroides fragilis in health and disease.bacteroides fragilis is generally believed to be stimulated by bile. although b. fragilis is rarely found in the human duodenum, it is relatively frequent in gall bladder infections. to investigate this paradox, the growth of b. fragilis in the human bile in both health and disease was studied, and compared with the effect of bovine and porcine bile. b. fragilis was stimulated by the bovine bile and inhibited by the porcine bile. the normal human bile was either bacteriostatic or inhibitory wher ...19883205170
[isolation of capnocytophaga in human infections]. 19883244931
phospholipase a2: a possible virulence factor of capnocytophaga ochracea. 19883268744
serum immunoglobulin g antibody to periodontal bacteria. 19883271028
infection with a gram-negative organism stimulates gingival collagenase production in non-diabetic and diabetic germfree rats. 19882846815
coaggregation of black-pigmented bacteroides species with other oral bacteria.coaggregation of bacteroides gingivalis and other black-pigmented bacteroides with several oral bacteria was studied with "reagent" strains specially prepared by methods that have been described previously. b. gingivalis coaggregated with veillonella, capnocytophaga and actinomyces spp., but not with any streptococcus spp. coaggregation of b. gingivalis with other bacteria was inhibited and reversed by lactose. of the asaccharolytic black-pigmented bacteroides, only b. gingivalis demonstrated an ...19892913312
capnocytophaga keratitis. 19892913817
susceptibility testing of danish isolates of capnocytophaga and cdc group df-2 bacteria.twelve capnocytophaga and seven df-2 strains were tested for their susceptibility to 14 antimicrobial agents using an agar dilution and an agar diffusion method. twenty-three other antibiotics were evaluated using the diffusion test only. all strains were fully susceptible to penicillin, ampicillin, cefuroxime, cefotaxime, erythromycin, clindamycin, chloramphenicol, doxycycline, rifamycin and ofloxacin using both methods. clindamycin, rifamycin and cefotaxime were most active. using agar dilutio ...19892914105
capnocytophaga canimorsus sp. nov. (formerly cdc group df-2), a cause of septicemia following dog bite, and c. cynodegmi sp. nov., a cause of localized wound infection following dog bite.cdc group df-2 is the vernacular name given to a slow-growing gram-negative bacterium that causes septicemia and meningitis in humans. infections frequently (one-third of cases) occur following dog bites or close contact with dogs or occasionally with cats. splenectomy and alcoholism appear to be strong predisposing factors for df-2 infection. in addition to 150 df-2 strains received for identification, we received 9 df-2-like strains; 6 were isolated from wound or eye infections, 3 of which wer ...19892915017
peritonitis involving capnocytophaga ochracea. 19892916537
[osseointegrated implants in clinical dentistry. follow up maintenance phase].the aim of this study is to determine the method of maintenance care for support the functional condition during long period. the present paper reports on the result of osseointegrated titanium fixture in four complete denture patients. the marginal soft tissue reactions were investigated at the 1st, 3rd, 6th, 12th and 18th month after prothetic restrations by clinical examination and microbiological observations. all abutments were surrounded by clinically in healthy gingiva, however most indiv ...19892489293
[bacteriological study of gingivitis in pubertal children. 2. subgingival microflora cultivable on the nonselective blood agar medium].the present study involved cultural and microscopic characterization of the subgingival microorganisms of pubertal children with gingivitis. samples were obtained from eight children 12-14 years of age. gram-positive organisms made up 56% of the isolates and included mainly actinomyces israelii, actinomyces naeslundii. gram-negative anaerobic rods constituted 25% of the isolates and bacteroides intermedius (9.7%) was most predominant species. the selective media permitted significantly lower rec ...19892489550
[sepsis caused by capnocytophaga ochracea in a patient with acute leukemia]. 19892490471
comparison of gc-lect and modified thayer-martin media for isolation of neisseria gonorrhoeae.a new selective medium, gc-lect, was compared with modified thayer-martin medium (mtm) for isolation of neisseria gonorrhoeae. cultures from 620 sexually transmitted disease clinic patients were directly inoculated onto both media, placed in candle extinction jars, and incubated. n. gonorrhoeae was isolated from 175 (29%) of 607 genital cultures, 3 (3%) of 88 pharyngeal cultures, and 6 (29%) of 21 rectal cultures. ten cultures were positive only on gc-lect, and 3 were positive only on mtm. in 3 ...19892501345
new selective medium for the isolation of neisseria gonorrhoeae.gc-lect, a new selective medium for the isolation of neisseria gonorrhoeae which contains five antimicrobial agents, was evaluated with stock cultures and with 500 clinical specimens. with stock cultures, vancomycin-resistant staphylococcus epidermidis that grew on modified thayer-martin medium (mtm) was inhibited on the new medium. also, vancomycin-susceptible strains of n. gonorrhoeae were much less inhibited on the new medium than on martin-lewis agar or mtm. with oropharyngeal cultures of he ...19892509512
bactericidal concentrations of chlorhexidine-digluconate, amine fluoride gel and stannous fluoride gel for subgingival bacteria tested in serum at short contact times.in vitro inhibitory and bactericidal concentrations in serum of chlorhexidine-digluconate, amine fluoride gel, stannous fluoride gel, stannous fluoride, metronidazole and amoxicillin were determined against bacteroides gingivalis, bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and capnocytophaga sputigena. the minimal inhibitory concentration was assessed by the agar dilution technique. the killing curves and minimal bactericidal concentration of the antim ...19892524581
[gene cloning of an actinobacillus actinomycetemcomitans y4 antigen that reacts to peripheral blood sera in advanced destructive periodontitis patients].actinobacillus actinomycetemcomitans (a.a.) has been implicated in the etiology of juvenile periodontitis and also of advanced destructive periodontitis (adp). it has been reported that the levels of the igg antibody against the a.a. in the peripheral blood sera of the adp patients were often high as well as those against the bacteroides gingivalis. to clone the genes of antigen reactive to the sera of the adp patients, we constructed a phage library of the a.a. strain y4 dna in lambda l47, whic ...19892533606
characterization of cdc group df-3 by cellular fatty acid analysis.fourteen strains of centers for disease control group df-3 bacteria were examined for cellular fatty acid composition to evaluate their chemical relatedness to known bacterial species and groups. the fatty acids were liberated from whole cells by base hydrolysis, methylated, and analyzed by capillary gas-liquid chromatography. all group df-3 strains possessed a distinct fatty acid profile which was characterized by large amounts (24%) of 12-methyltetradecanoate (a-c15:0), moderate amounts of sat ...19892542365
microbiota and crevicular fluid collagenase activity in the osseointegrated dental implant sulcus: a comparison of sites in edentulous and partially edentulous patients.the soft tissues adjacent to osseointegrated dental implants (oii) were investigated using clinical, biochemical and microbiological methods. tooth and implant crevices were compared in 15 partially edentulous patients, examining 28 peri-implant and 19 periodontal sites, and in 6 edentulous patients, examining 13 implant sites. sites were classified by standard periodontal indices; the crevicular fluid flow determined; crevicular fluid was collected for collagenase assays; and the subgingival ba ...19892542514
dysgonic fermenter-2 meningitis simulating viral meningitis. 19892569590
use of adhesin-specific monoclonal antibodies to identify and localize an adhesin on the surface of capnocytophaga gingivalis dr2001.monoclonal antibodies capable of inhibiting coaggregation between capnocytophaga gingivalis dr2001 and actinomyces israelii pk16 were used to identify the adhesin on c. gingivalis that mediates the interaction. the monoclonal antibodies were used to demonstrate that a 140-kilodalton polypeptide found in the outer membrane of c. gingivalis was the adhesin responsible for coaggregation. a coaggregation-defective mutant that was unable to coaggregate with a. israelii lacked this large polypeptide. ...19892572552
an in vitro method to study the adherence of oral bacteria to hela cells.exfoliated buccal epithelial cells have been widely used in microbial adherence studies, but present a number of problems due to their variable nature and to contamination with indigenous bacteria. an adherence assay was developed using hela cell monolayers which were washed with buffer, or treated with saliva or serum to mimic buccal or crevicular epithelial cells, respectively. a total of eighteen strains of oral bacteria tested showed a low affinity for untreated hela cells, but most strains ...19892607993
t lymphocyte disorder after capnocytophaga ochracea endocarditis.capnocytophaga species are gram-negative rods which may cause disease in both non-immunocompromised and immunocompromised hosts. we describe a case of endocarditis due to capnocytophaga ochracea in a non-immunocompromised patient with a decrease of blood cd4/cd8 ratio and lymphocyte proliferative response to cona during infection. in vitro experiments showed that c. ochracea decreased lymphocyte proliferation to mitogens (cona, pha), cell surface cd4 antigen and il2 receptor expression on periph ...19892613326
the predominant cultivable microbiota of crevicular epithelial cells.the purpose of the present investigation was to compare the composition of the predominant cultivable microbiota associated with gingival crevicular epithelial cells with that of the unattached microbiota recovered from the same site. samples were taken from 2 diseased sites from 8 periodontal patients, by scraping the epithelial lining of the pocket with a curette. the epithelial cells were separated from the unattached subgingival bacteria by centrifugation in a reduced 50% percoll density gra ...19892628861
antimicrobial action of natural substances on oral bacteria.the antimicrobial action of natural substances was investigated in vitro against oral bacteria including streptococcus sp., actinomyces sp., actinobacillus sp., bacteroides sp., capnocytophaga sp., eikenella sp., fusobacterium sp. and propionibacterium sp. among the natural substances tested, hinokitiol was the most inhibitory to oral bacteria. cinnamon bark oil, papua-mace extracts, and clove bud oil in spice extracts were also inhibitory against many oral bacteria. egg white lysozyme exhibited ...19892637783
[effects of periodontopathic bacterial components on phagocytic activity of rat peritoneal macrophages. examination using elisa].this study examined the phagocytic activity of rat peritoneal resident macrophages to determine the movement of macrophages in local inflammation in periodontal disease. we studied phagocytic activity by enzymelinked immunosorbent assay (elisa) and used the peroxidase-anti-peroxidase soluble complex (pap; soluble immune complex) as a marker in. we also determined the basic conditions of this examination and studied the effects of bacterial components and the supernatants of sonicated periodontop ...19892637908
[antibiotic therapy of bacterial infections of the oral cavity].more than 300 commencial bacterial species may be found in the oral cavity. other microorganisms, such as mycoplasms, mycetes, protozoa and viruses are present as well. the virulency of the saprofites and additional contamination by outside microorganisms are factors determining the development of infectious process in the oral tissues. moreover, streptococci and anaerobes are the most frequent aetiology agents. the antibiotic therapy should comply with the general treatment criteria, on the one ...19892638255
structural analysis of lipopolysaccharides from eikenella corrodens by use of murine monoclonal antibodies.the structure of lipopolysaccharides (lpss) of eikenella corrodens was analyzed with prepared murine monoclonal antibodies. a common core epitope was found in three of seven lps preparations from e. corrodens strains and fusobacterium nucleatum atcc 25586. four e. corrodens lpss were found to possess an o-side-chain epitope which cross-reacted with lpss from fusobacterium necrophorum atcc 25286 and capnocytophaga ochracea m-12. lipid a of e. corrodens lps shared an epitope common among lpss from ...19892643582
relationship of subgingival plaque flora to lysosomal and cytoplasmic enzyme activity in gingival crevicular fluid.examining the relationships among indicators of the acute inflammatory response in gingival crevicular fluid (gcf) and specific bacterial species in subgingival plaque may provide indications of which bacterial species or groups of species may be associated with potentially destructive host-derived processes. here we report on the relationship of the subgingival plaque flora to the activity of mammalian forms of the enzymes beta-glucuronidase (beta g), lactate dehydrogenase (ldh), and arylsulfat ...19892656765
isolation of a coaggregation-inhibiting cell wall polysaccharide from streptococcus sanguis h1.coaggregation between streptococcus sanguis h1 and capnocytophaga ochracea atcc 33596 cells is mediated by a carbohydrate receptor on the former and an adhesin on the latter. two methods were used to release the carbohydrate receptor from the gram-positive streptococcus, autoclaving and mutanolysin treatment. the polysaccharide released from the streptococcal cell wall by either treatment was purified by ion-exchange chromatography; this polysaccharide inhibited coaggregation when preincubated w ...19892661543
actinobacillus actinomycetemcomitans serotypes and leukotoxicity in korean localized juvenile periodontitis.actinobacillus actinomycetemcomitans is though to play an important role in the pathogenesis of localized juvenile periodontitis (ljp). preliminary data suggested that the serotype distribution of a. actinomycetemcomitans in korea and the united states differ. this study evaluated a. actinomycetemcomitans prevalence, serotype distribution, and leukotoxicity in korean ljp patients by culture, enzyme-linked immunosorbent assay, indirect immunofluorescence, and lactate dehydrogenase release from po ...19892677302
effect of 0.12% chlorhexidine on bacterial recolonization following periodontal surgery.bacterial plaque accumulation following periodontal surgery has been directly associated with delayed and altered surgical wound healing. successful antimicrobial treatment following periodontal surgery depends upon the elimination and suppression of wound associated microorganisms. highly effective antimicrobials should also prevent recolonization of periodontopathogens. in this investigation, the antimicrobial effect of a 0.12% chlorhexidine gluconate mouthrinse (peridex) on bacterial recoloni ...19892681676
[correlation between subgingival microflora and peptidase activity in periodontal pockets measured with synthetic substrates].the activity of peptidases in periodontal pockets of patients were examined by using four different synthetic enzyme substrates, and surveyed their correlation with the microbial populations of subgingival plaque and clinical symptoms. the substrates were (1) alpha-n-benzoyl-dl-arginine-beta-naphthylamide (bana), (2) n-carbobenzoxy-glycylglycyl-arginine-4-methoxy-beta-naphthylamide, (3) alpha-n-benzoyl-l-arginylglycyl-l-phenylalanyl-l-proline-4-methoxy -beta-naphthylamide and (4) n-carbobenzoxy- ...19892700363
subgingival microflora, dental and periodontal conditions in patients with hereditary fructose intolerance.hereditary fructose intolerance (hfi) is a rare autosomally recessive disease which leads to severe hypoglycemia. the fructose-free diet of these patients apparently influences dental health. half of the patients are free of caries, but there are no reports on their periodontal condition nor on the composition of their subgingival microflora. therefore 18 patient with hfi were examined for the following parameters: radiographic bone loss, caries rate, gingival bleeding, occurrence of calculus, p ...19892704977
the in vitro effects of chlorhexidine on subgingival plaque bacteria.the purpose of this study was to determine the susceptibility to chlorhexidine of a range of bacteria which may be isolated from subgingival plaque. in addition, the effect of chlorhexidine on the survival of bacteria in subgingival plaque samples from patients with chronic inflammatory periodontal disease was investigated. the minimum inhibitory concentration (mic) of chlorhexidine for 52 strains of bacteria ranged from 8 to 500 micrograms/ml. the modal value of the mic was found to be 62 micro ...19892715364
periodontal condition and microbiology of healthy and diseased periodontal pockets in type 1 diabetes mellitus patients.on the basis of glycosylated hemoglobin (hba1c) values, 22 type 1 (insulin-dependent) diabetic adults were grouped into patients with near normal (hba1c less than or equal to 7.7%) and poor (hba1c greater than or equal to 9.9%) metabolic control. a total of 44 subgingival sites were examined for actinobacillus actinomycetemcomitans, black-pigmented bacteroides species and capnocytophaga species. no significant difference could be demonstrated between patients in the 2 test groups with regard to ...19892723104
humoral immune response to selected subgingival plaque microorganisms in insulin-dependent diabetic children.juvenile diabetics have been shown to have an increased susceptibility to gingivitis and periodontitis following puberty. however, little data are available on changes in the microbial flora that occur at the onset of puberty. this study was performed to determine if antibacterial antibody titers to selected periodontal disease-associated microorganisms might be helpful in revealing changes in plaque flora at the onset and conclusion of puberty. sera was obtained from 35 subjects (ages 7 to 18 y ...19892724033
the effect of subminimal inhibitory concentrations of antimicrobial agents on three bacterial mixtures.a test tube technique was developed to screen bacterial mixtures to detect interbacterial interactions that play a role in determining sensitivity to antimicrobial agents. we found 3 mixtures where these bacterial interactions change the sensitivity to antimicrobials or change the proportions of each bacterial species in the mixture. the mixtures were: fusobacterium nucleatum 102.3 and bacteroides endodontalis atcc 35406; f. nucleatum 102.3 and b. endodontalis bn11 a-f; and capnocytophaga ochrac ...19892762019
coaggregation of fusobacterium nucleatum, selenomonas flueggei, selenomonas infelix, selenomonas noxia, and selenomonas sputigena with strains from 11 genera of oral bacteria.twenty-eight strains of fusobacterium nucleatum and 41 selenomonas strains, including s. sputigena (24 strains), s. flueggei (10 strains), s. infelix (5 strains), and s. noxia (2 strains), were tested for their ability to coaggregate with each other and with 49 other strains of oral bacteria representing actinobacillus, actinomyces, bacteroides, capnocytophaga, gemella, peptostreptococcus, porphyromonas, propionibacterium, rothia, streptococcus, and veillonella species. selenomonads coaggregated ...19892777378
in vitro activity of a chlorhexidine-containing mouthwash against subgingival bacteria.three hundred and fifteen subgingival isolates were tested for their susceptibility to a mouthwash containing 0.2% chlorhexidine. the majority of strains tested were susceptible at high dilution although some strains of s. mitior, s. sanguis, and capnocytophaga were relatively resistant. it was concluded that, on the basis of these in vitro results, the mouthwash should have potential for subgingival use in the treatment of chronic periodontitis.19892795419
the relationship of serum igg antibody titers to periodontal pathogens to indicators of the host response in crevicular fluid.in this study; the relationship of indicators of the local host response in gingival crevicular fluid (gcf) to the serum antibody titer to periodontal pathogens was examined. 15 patients with chronic adult periodontitis were studied. gcf was collected and analyzed for the total amount of igg, igm, the lysosomal enzyme b-glucuronidase (bg) and alpha-2-macroglobulin (alpha 2m). at the same examination, serum from these patients was collected, and enzyme-linked immunosorbent assays used to determin ...19901696949
an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel sections.an in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ...19902087341
the incidence of periodontopathic microorganisms in young children.thirty-six children in 4 cohorts of 0-1 week, 1-6 months, 1-2 years and 2-2 1/2 years of age were examined for the presence of black-pigmented bacteroides species and some other bacteria suspected of being involved in the subsequent development of periodontal destruction. none of the sought-after bacteria were detected in the first week of life. bacteroides intermedius and bacteroides melaninogenicus were detected as early as 1 month after birth. both of these bacteria were detected in 16-37% of ...19902087345
identification of the rhamnose-sensitive adhesin of capnocytophaga ochracea atcc 33596.the coaggregation of the gram-negative microorganism capnocytophaga ochracea atcc 33596 with several gram-positive bacteria, including streptococci, actinomyces and rothia, is mediated by rhamnose-sensitive adhesin(s). in the present study, mabs against the c. ochracea adhesin(s) were prepared. these antibodies inhibited all the rhamnose-sensitive interactions, indicating that they recognize epitopes at or near the rhamnose binding site of the adhesin. the monoclonals served as probes in immunob ...19902088216
humoral immune responses in experimental gingivitis in rats.serum igg, igm, and iga antibody levels to extracts of rat dental plaque and five oral bacteria (haemophilus actinomycetemcomitans y-4, bacteroides gingivalis 381, bact. intermedius atcc 25261, capnocytophaga sp. m-12, eikenella corrodens odu) were determined by elisa. in addition, the presence of rat dental plaque and oral bacterial components in the inflamed gingival tissue was studied using immunofluorescence techniques. serum and gingival tissue samples were obtained from odu plaque-suscepti ...19902088226
the immediate efficacy of adjunctive doxycycline in treatment of localized juvenile periodontitis.a randomized, double-blind, placebo-controlled study on the immediate clinical and microbiological efficacy of doxycycline (100 mg for 14 days) was carried out to determine the benefit of adjunctive medication in 16 patients with localized juvenile periodontitis. measurements of gingival fluid flow, probing depths, bleeding on probing and suppuration were determined at 2 periodontal sites with and 2 without radiographic attachment loss, at weeks 0, 1, 3 and 8. subgingival bacterial samples were ...19902088233
killing of oral, gram-negative, facultative bacteria by the rabbit defensin, np-1.oral, gram-negative, facultative bacteria, including actinobacillus actinomycetemcomitans, eikenella corrodens, and capnocytophaga spp. have been associated with destructive periodontal infection. neutrophils play a critical role in defending the periodontium against destructive infection. defensins are antimicrobial peptides that have been isolated in human, rabbit, guinea pig, and rat leukocytes that may constitute an important nonoxidative mechanism of killing. the purpose of this study was t ...19902098709
sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria.a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ...19902108991
studies of the antibacterial activity of plant extracts and their constituents against periodontopathic bacteria.plant extracts and their constituents were tested for antibacterial activity against periodontopathic bacteria, including actinobacillus, capnocytophaga, fusobacterium, eikenella and bacteroides species. the essential oils of two labiatae plants, mosla chinensis maxim. and pogostemon cablin benth., and five terpenoids, hinokitiol, thymol, carvacrol, patchoulialcohol and pogostone, showed antibacterial activity. the terpenoids were especially effective against bacteroides species.19902133438
[a rapid diagnosis (sk-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. study on the characterization of the sk-013 and the enzyme specificity].we present studies for development of an enzymatic diagnostic method for periodontitis. a rapid and sensitive diagnostic method named sk-013 was provided by sunstar inc. to evaluate the peptidase activities specifically derived from periodontopathic bacteria such as bacteroides gingivalis, bacteroides forsythus and treponema denticola and some strains of capnocytophaga species. the results obtained indicated the specificity of the enzymatic reaction in this system.19902133684
[studies on plaque formed on implants].in vivo plaque formation on implant materials was studied. when different implant materials were set on the gingiva, the number of adhering viable bacteria depended on material surface properties 4 hours after setting, but not 48 hours after setting. the formation of pellicle-like thin layers and subsequent covering by lamellarly formed plaque were observed on the surfaces of all materials. streptococcus species were predominant at the 4-hour setting time but anaerobes increased at the 48-hour s ...19902134753
[chemical structure and immunobiological activities of peptidoglycan isolated from capnocytophaga species].the chemical structure and immunobiological activities of the cell wall peptidoglycan isolated from capnocytophaga species was investigated. peptidoglycan was isolated from capnocytophaga species strain se2-2 by boiling in 4% sodium dodecyl sulfate and by digestion with pronase, trypsin and alpha-amylase. analysis of amino acids and amino sugars of the peptidoglycan revealed that glucosamine, muramic acid, d-glutamic acid, alanine, and diaminopimelic acid (a2pm) were the principal components. se ...19902134888
prepubertal periodontitis: a review of diagnostic criteria, pathogenesis, and differential diagnosis.this paper reviews the literature on prepubertal periodontitis (pp) according to the definition and the classification of pp by page. the generalized form of pp (g-pp) defined by page was identified as the oral manifestation of a systemic disease called leukocyte adhesion deficiency (lad) and thus the pathogenesis is known at the molecular level. on the other hand, the localized form of pp (l-pp) is a disease defined by clinical criteria and its pathogenesis is not known at the molecular level. ...19902137170
[capnocytophaga meningitis]. 19902138745
evaluation of the activity of four antimicrobial agents using an in vitro rapid micromethod against oral streptococci and various bacterial strains implicated in periodontitis.the activity of various antibacterial agents (amoxicillin, josamycin, doxycycline and metronidazole) was established in vitro using a rapid micromethod. the activity of these agents, which are widely used in oral medicine, was evaluated against microorganisms responsible for periodontitis and bucco-dental infections. their action against alpha-hemolytic streptococci (including pneumococci) which make up the majority of the indigenous oral flora was also tested. amoxicillin was found to be effect ...19902142728
microbial changes associated with the development of puberty gingivitis.in this study, longitudinal changes in the composition of the subgingival microbiota of children between the ages of 11 and 14 and their association with changes of clinical parameters describing gingival health were investigated. during 4 years, subgingival microbial samples were taken in 22 boys and 20 girls 10 times. at the same time the gingival bleeding tendency was recorded by the papillary bleeding index (pbi). a total of 840 samples was evaluated using darkfield microscopy and anaerobic ...19902148945
use of the rapid-ana system and sodium polyanetholesulfonate disk susceptibility testing in identifying haemophilus ducreyi.haemophilus ducreyi has traditionally been difficult to identify. we have utilized simple test methods to identify 19 fresh isolates obtained during a recent outbreak of chancroid in houston and six strains of h. ducreyi from other outbreaks. tests were performed from growth on chocolate agar after 48 h of incubation at 35 degrees c with increased humidity and co2. all isolates exhibited typical colonial morphology and gram stain. isolates were catalase negative and oxidase and nitrate positive ...19902153697
preferential adsorption of human neutrophil myeloperoxidase isoform iii by oral bacteria.neutrophil myeloperoxidase (mpo) adsorbs to bacteria as a pre-requisite for killing by the mpo/hydrogen-peroxide/chloride system. three chromatographically distinct isoforms of mpo (mpo i, mpo ii, and mpo iii) have been isolated from human neutrophils. the purpose of this study was to determine whether oral bacteria--including actinobacillus actinomycetemcomitans, capnocytophaga sputigena, haemophilus aphrophilus, and eikenella corrodens--differentially adsorb the three major isoforms of mpo fro ...19902172341
detection of microbial trypsin-like enzymes by use of an agar gel.certain oral micro-organisms associated with periodontal diseases elaborate trypsin-like enzymes. this study describes a single assay for these enzymes, based on the incorporation of the synthetic substrate n-alpha-benzoyl-dl-arginine-p-nitroanilide (bapna) into ion-agar gel. the system can provide quantitative or qualitative data within 24 h and is suitable for use in the dental clinic.19902172751
polymorphonuclear leukocyte phagocytosis of capnocytophaga ochracea in three-dimensional plasma clots.we have studied the ability of human polymorphonuclear leukocytes (pmn) to phagocytose capnocytophaga ochracea in three-dimensional fibrin meshworks. phagocytosis was assessed in three systems: (1) the pmn and bacteria were mixed together with plasma and clotted; 60 +/- 13% phagocytosis occurred after 60 min; (2) pmn were overlaid on clots containing bacteria; the pmn migrated into the clot and after 60 min 52 +/- 7% phagocytosis was seen; (3) pmn had to migrate from within one clot into a secon ...19902200793
capnocytophaga canimorsus (formerly df-2) infections: review of the literature. 19902203978
generalized juvenile periodontitis: report of a familial case followed for 5 years.the case of a family, followed for 5 years and showing an exceptionally high prevalence of generalized juvenile periodontitis (gjp), is presented. two siblings were affected by a severe form of gjp meanwhile the dycorial twin of one was periodontally healthy. both the affected siblings showed infection by actinobacillus actinomycetemcomitans (aa), but only one presented a reduced chemotaxis of the peripheral pmns. the dycorial twin consistently displayed a freedom from aa and a reduction in the ...19902213470
novel beta-lactamase from capnocytophaga sp.a novel beta-lactamase activity which confers resistance to expanded-spectrum cephalosporins and penicillins has been found in strain ic 5/21 of capnocytophaga spp. enzyme activity migrated at a molecular size of 38,000 daltons and at an isoelectric point of 3.6, with a minor band at 4.1. kinetic studies suggested that it belonged to richmond and sykes beta-lactamase class 1c. isoelectric focusing could be achieved only if a nonionic detergent was added to the gel, suggesting the presence of a h ...19902221858
[ex-vivo study of the capacity of bacteria of the genus capnocytophaga to adhere to human buccal epithelial cells].the bacteria of the genre capnocytophaga are part of the subdominant flora of the oral cavity. for diverse microorganisms it has been proved that the adhesion constitutes the first step of the colonization of a place leading to the eventual pathology. the adhesion capacity to human epithelial cells (keratinised and non) of eight strains of genus capnocytophaga has been studied. all strains appear to have a very weak capacity of adhesion. this diministe can be originated from the fact that the ho ...19902237107
in vitro sensitivity of oral, gram-negative, facultative bacteria to the bactericidal activity of human neutrophil defensins.neutrophils play a major role in defending the periodontium against infection by oral, gram-negative, facultative bacteria, such as actinobacillus actinomycetemcomitans, eikenella corrodens, and capnocytophaga spp. we examined the sensitivity of these bacteria to a mixture of low-molecular-weight peptides and highly purified individual defensin peptides (hnp-1, hnp-2, and hnp-3) isolated from human neutrophils. whereas the capnocytophaga spp. strains were killed significantly by the mixed human ...19902254020
changes in subgingival microbiota during puberty. a 4-year longitudinal study.it was the purpose of the present investigation to monitor the composition of the subgingival microbiota at selected sites in individuals passing through puberty and to correlate observed changes with the development of pubertal maturation. between the ages of 11 and 14 years, pubertal and skeletal maturation was monitored annually in 22 boys and 20 girls. during this time, subgingival microbial samples were taken every 4th to 5th month (10 times in 4 years) mesially of the upper first molars. h ...19902262580
[capnocytophaga septicemia during bone marrow transplantation. apropos of 2 cases].two cases of septicemia due to capnocytophaga in pediatric bone marrow recipients are reported. these gram negative rods, which are part of the normal buccal flora, cause periodontitis and localized or systemic infections, usually in immunocompromised hosts. severe, prolonged neutropenia and mucitis due to chemotherapy and/or radiotherapy are risk factors for this opportunistic infection. recovery can be achieved with most of the drug combinations including a beta-lactam used in hematology.19902291593
microbial patterns in pooled subgingival plaque samples from young adults with advanced marginal periodontitis.different bacterial profiles of subgingival plaque have been described for different clinical categories of marginal periodontitis. in the present investigation the subgingival microflora was studied in young adults with advanced marginal periodontitis. from 12 patients pooled subgingival plaque samples from 5 advanced stages of diseased sites were examined by direct differential phase-contrast microscopy and by cultivation on enriched and selective media. the proportions of the following genera ...19902293348
suspected periodontopathogens in erupting third molar sites of periodontally healthy individuals.29 periodontally healthy subjects (11 female and 18 male) with a mean age of 24 years (range 19 to 38 years) and with partially erupted lower third molars participated in this study. 18 subjects demonstrated no signs or symptoms of acute inflammation and were without pain (group a). 5 subjects showed redness of the pericoronal tissues and experienced pain upon palpation (group b). 6 subjects suffered from acute pain and exhibited formation of pus (group c). microbiological samples were taken fro ...19902295708
capnocytophaga canimorsus as the cause of a chronic corneal infection. 19902301546
capnophilic and anaerobic bacteremia in neutropenic patients: an oral source.the currently accepted empiric antibiotic therapy for bacterial infections in neutropenic patients may not cover the possibility of capnophilic and anaerobic bacteremia. many of these infections develop in patients with severe mucositis or periodontitis, and the type of organisms recovered also suggests an oral source of infection. we present two cases of bacteremia in neutropenic patients who had been empirically treated with ceftazidime and piperacillin plus amikacin. in the first case a beta- ...19902305182
capnocytophaga keratitis associated with poor dentition and human immunodeficiency virus infection. 19902309871
dysgonic fermenter 3-associated abscess in a diabetic patient.we report a case in which a strain of the u.s.a. centers for disease control (cdc) dysgonic fermenter (df) 3, together with citrobacter freundii, was isolated from an abscess in a diabetic patient. df 3 may be easily overlooked due to its fastidious nature, a characteristic shared with two former df groups now placed in the genus capnocytophaga. to our knowledge, this is the first european case report of df 3-associated infection.19902341734
combination and single-agent empirical antibacterial therapy for febrile cancer patients with neutropenia and mucositis.the role of mucositis in infectious complications in the patient with cancer is poorly understood. consequently, neither the presence nor the severity of mucositis is routinely considered in the selection of specific antibacterial agents for the initial empirical therapy of the febrile cancer patient. in a study of children receiving remission induction chemotherapy for acute nonlymphocytic leukemia, the number of febrile days correlated more closely with the degree of mucositis than with the nu ...19902342588
protein profiles of capnocytophaga species.ninety-seven strains of capnocytophaga isolated from the oral cavity and the type strains of c. ochracea, c. sputigena and c. gingivalis were compared by one-dimensional sds-page of whole cell proteins. the protein patterns were highly reproducible and were used as the basis for numerical taxonomic analysis. the clusters containing the type strains of c. ochracea and c. sputigena segregated at the 78% similarity level. some of the eight clusters obtained at this level showed good correlation wit ...19902351620
serum antibodies and loss of periodontal bone in patients with rheumatoid arthritis.the number of teeth, % of alveolar bone loss, serum igg, and serum antibodies to bacteroides gingivalis, capnocytophaga ochracea and eubacterium saburreum were recorded in 37 patients diagnosed with rheumatoid arthritis (ra) and in an age- and sex-matched control group of 37 individuals free from ra. the ra group had a significantly increased loss of teeth and loss of alveolar bone compared to the control group. the ra patients also had a significantly increased level of serum igg. in the total ...19902355094
[a case of septicemia due to capnocytophaga ochracea beginning post radiation therapy for lung cancer].a 46-year-old man was admitted to our hospital on mar. 16, 1988 with the chief complaint of productive cough. the chest roentgenogram and tomogram showed a tumorous shadow in the right upper lobe, accompanied with stenosis of the trachea and the right main bronchus. bronchofiberscopic examination confirmed a nodular tumor protruding into the lower part of the trachea from the right lateral wall and nearly complete obstruction of the right main bronchus. pathological specimen obtained by transbro ...19902358715
precipitous hypotension in the emergency department caused by capnocytophaga canimorsus sp nov sepsis.a 20-year-old man presented to the emergency department (ed) with an injured right hand, fever, and a history of dog exposure. this splenectomized individual developed hypotension less than 90 minutes after arriving in the ed with normal vital signs. he later developed overwhelming sepsis, gangrene, disseminated intravascular coagulation (dic), respiratory insufficiency, retroperitoneal hematoma, and renal insufficiency. blood cultures grew capnocytophaga canimorsus sp nov (formerly dysgonic fer ...19902363753
capnocytophaga infection involving a portal-systemic vascular shunt.capnocytophaga sp., a microaerophilic gram-negative isolate of the human oral cavity, has previously been reported to cause sinusitis, empyema, wound infections, conjunctivitis, subphrenic abscess, osteomyelitis, bacteremia, cervical abscess, and endocarditis. we report the unusual case of infection with this organism at the anastamotic site of a splenorenal portosystemic vascular shunt. in this case, the pathogenesis is presumed to be bacteremia related to mucosal trauma from endoscopic injecti ...19902364847
development of a diagnostic test for anaerobic periodontal infections based on plaque hydrolysis of benzoyl-dl-arginine-naphthylamide.treponema denticola, porphyromonas (bacteroides) gingivalis, and bacteroides forsythus are among the anaerobic species frequently associated with adult forms of periodontal disease. these organisms hydrolyze the synthetic peptide benzoyl-dl-arginine-naphthylamide (bana), and such enzyme activity can be detected in the plaque and related to clinical disease and the presence of spirochetes. in this investigation, the liquid bana assay was compared with a commercially developed bana assay which emp ...19902380379
structure of a streptococcal adhesin carbohydrate receptor.interactions between complementary protein and carbohydrate structures on different genera of human oral bacteria have been implicated in the formation of dental plaque. the carbohydrate receptor on streptococcus sanguis h1 (one of the primary colonizing species) that is specific for the adhesin on capnocytophaga ochracea atcc 33596 (a secondary colonizer) has been isolated from the streptococcal cell wall, purified, and structurally characterized. the hexasaccharide repeating unit of the polysa ...19902387843
beware of the dog: meningitis in a splenectomised woman.purulent meningitis was diagnosed in a 75-yr-old splenectomised woman nine days after a dog bite. the original wound was apparently uninflamed. the causative microorganism proved to be a dysgonic fermenter 2 (df-2) bacterium (renamed capnocythophaga canimorsus). this is a recently recognised gram-negative bacterium, belonging to the normal canine mouth flora, to which asplenic individuals seem to be particularly susceptible.19902395498
capillary gas chromatography of cellular carbohydrates as a means for the differentiation of fastidious, slow-growing or anaerobic gram-negative bacteria--a review.the phenotypic differentiation of microaerophilic or anaerobic gram-negative bacteria of human origin is traditionally based on the analysis of metabolic characteristics. due to outstanding growth requirements of many kinds of parasitic bacteria, e.g. of the facultatively and strictly anaerobic members of the human periodontal flora, conventional biochemical tests are time-consuming and sometimes lead to doubtful results. commercial diagnostic test kits often produce misidentifications because o ...19911755919
capnocytophaga species infections in children. 19911766712
df2 septicaemia. 19911774757
[bacteriological examination of infections in the field of oral surgery].etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ...19911791332
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