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[bacterial examination in periodontal diseases]. 19846597612
rapid identification of capnocytophaga isolated from septicemic patients.four capnocytophaga strains from blood cultures of immunocompromised patients with malignant disease and the type strains of three capnocytophaga species were examined and compared to strains representing five other genera that are hard to differentiate from capnocytophaga. with three rapid identification methods, negative catalase and oxidase reactions and positive onpg assay, capnocytophaga was easily separated from eikenella corrodens, actinobacillus actinomycetemcomitans, cardiobacterium hom ...19846468367
capnocytophaga ochracea infection: two cases and a review of the published work.bacteria of the genus capnocytophaga are recently recognised pathogens which may cause oral disease and subsequent septicaemia in the immunocompromised host. we present two cases of infection caused by capnocytophaga ochracea; a soft tissue infection in an immunologically normal patient and an episode of septicaemia in a child with leukaemia. the microbiology, pathogenicity, and antimicrobial susceptibility of the genus capnocytophaga are reviewed.19846470184
bacteriology of experimental gingivitis in children.children are more resistant to gingivitis than are adults. to determine possible differences in their periodontal floras, an experimental gingivitis study, identical in design to one reported earlier with young adults, was conducted with four 4- to 6-year-old children. the incidence of sites that developed gingival index scores of 2 in children was less than one-third of the incidence observed in adults. the composition of the flora of each child was statistically significantly different from th ...19846480100
the bone-resorbing activities in tissue culture of lipopolysaccharides from the bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis and capnocytophaga ochracea isolated from human mouths.the activities of lipopolysaccharides (lps) were assessed by measuring the calcium release from mouse calvaria in vitro and compared to that of lps from salmonella typhimurium. stimulation of bone resorption was maximal at an lps concentration of 10 micrograms/ml and at this dose all oral lps preparations showed similar levels of activity and less than that of lps from s. typhimurium. only s. typhimurium lps and b. gingivalis lps retained bone-resorbing activity at 0.1 microgram/ml. no bone-reso ...19846362631
septic arthritis involving capnocytophaga ochracea.septic arthritis of the knee developed in a 21-month-old child. the causative organism, isolated from two separate arthrocenteses, was identified as capnocytophaga ochracea morphologically and by biochemical reactions. previous human infections (bacteremias) have occurred in granulocytopenic hosts with concomitant oral pathology including periodontitis and gingivitis. no abnormalities of oral hygiene were present in this patient, and granulocyte numbers were normal or elevated. eradication of th ...19846715520
disseminated actinomycosis associated with infection by capnocytophaga species. 19846725998
two cases of capnocytophaga bacteremia, one with endocarditis. 19846733613
immunoglobulin g response to subgingival gram-negative bacteria in human subjects.serum and gingival crevicular fluid from normal healthy adults and patients with periodontitis were screened for immunoglobulin g antibodies to antigens from bacteroides gingivalis 381, bacteroides intermedius 24, bacteroides loescheii atcc 15930, fusobacterium nucleatum atcc 25586, eikenella corrodens 1073, actinobacillus actinomycetemcomitans atcc 29522, and capnocytophaga sp. strain m-12. immunoglobulin g antibody titers to the antigens were measured by an enzyme-linked immunosorbent assay. t ...19846735473
capnocytophaga infection: a risk in the immunocompromised host. 19846741389
capnocytophaga species: infections in nonimmunocompromised and immunocompromised hosts.retrospective review of isolates of capnocytophaga, a genus of capnophilic gram-negative bacilli, referred to the massachusetts state laboratory institute in boston revealed 31 patients with infection due to capnocytophaga, 16 in nonimmunocompromised hosts. these infections included empyema (three patients), lung abscess (one), sinusitis (one), conjunctivitis (three), subphrenic abscess (one), wound (three), osteomyelitis (one), and bacteremia (three). two of the wound infections were closed-fis ...19853965585
lysozyme-mediated aggregation and lysis of the periodontal microorganism capnocytophaga gingivalis 2010.the ability of lysozyme to aggregate and lyse the gram-negative capnophilic periodontal microorganism capnocytophaga gingivalis 2010 was monitored optically at 540 nm. both hen egg white and chromatographically purified human lysozymes had significant but similar aggregation potentials for both logarithmic- and stationary-phase bacteria. in general, an increase in enzyme concentration resulted in a graded increase in both the initial and maximum changes in turbidity which occurred during the rea ...19853967924
degradation of human immunoglobulins g and m and complement factors c3 and c5 by black-pigmented bacteroides.strains of bacteroides, capnocytophaga and fusobacterium were examined by immunological methods for their ability to degrade the human serum proteins igg, igm, c3 and c5. the proteolytic activity of the strains was measured in terms of the breakdown of serum into trichloroacetic acid-soluble material. only black-pigmented bacteroides strains showed proteolytic activity. strains of b. gingivalis degraded igg, igm, c3 and c5, strains of b. intermedius igg and c3, strains of b. endodontalis c3 and ...19853968707
capnocytophaga isolated from the endometrium as a cause of neonatal sepsis. a case report.capnocytophaga, a genus of gram-negative, anaerobic organisms, was isolated from the endometrium of a postpartum woman and the blood of the neonate. this unusual organism, usually isolated from the oral cavity, is sensitive to most antibiotics except the aminoglycosides.19853973864
capnocytophaga keratitis.we report two cases of bacterial keratitis caused by capnocytophaga, a genus of capnophilic gram negative bacilli. both responded to topical and subconjunctival clindamycin.19853978064
purification and chemical characterization of an exopolysaccharide isolated from capnocytophaga ochracea.purification and chemical characterization of an immunosuppressive exopolysaccharide from capnocytophaga ochracea strain 25 are described. this polysaccharide was extracted from spent culture medium by cold ethanol precipitation. purification was accomplished by trichloroacetic acid and pronase treatments in combination with diethylaminoethyl-sepharose and concanavalin a-sepharose chromatography. purity of the exopolysaccharide was ascertained by polyacrylamide gel electrophoresis using periodic ...19853986710
comparative bacteriology of juvenile periodontitis.statistical comparisons of the floras associated with juvenile periodontitis, severe periodontitis, and moderate periodontitis indicated that differences in the bacterial compositions of affected sites in these populations were not statistically significant. the subgingival flora of affected juvenile periodontitis sites was statistically significantly different from the adjacent supragingival flora and from the subgingival floras of people with healthy gingiva and of children with developing (ex ...19853988344
isolation of neisseria elongata and of capnocytophaga ochracea from vaginal specimens. 19854008966
capnocytophaga: a pathogen in immunocompetent hosts. 19854008992
induction of spheroplasts in capnocytophaga ochracea.a gentle technique for preparing spheroplasts of capnocytophaga ochracea strain 25 is described. cells in the exponential phase were washed with 1.0 m-nacl, agitated in 1.0 m-nacl for 2 h at 30 degrees c and exposed to lysozyme in a tris/salts buffer, ph 7.0. this procedure resulted in 98% spheroplast formation with complete removal of the peptidoglycan layer as detected by both phase-contrast and electron microscopy in combination with chemical analysis.19854020345
congenital bacteremia due to capnocytophaga. 19854022809
peritonitis involving capnocytophaga ochracea.spontaneous bacterial peritonitis, due to capnocytophaga ochracea, developed in a 65-year-old alcoholic patient with extensive cirrhosis and ascites. previously reported human infections with this organism have included peridontal diseases, septicemia, and arthritis. this is the first report of spontaneous bacterial peritonitis associated with capnocytophaga species.19854028664
assessment of serum antibody patterns and analysis of subgingival microflora of members of a family with a high prevalence of early-onset periodontitis.in a study of members of a large family with a high prevalence of early-onset periodontitis, we sampled the subgingival microflora and characterized 40 isolates from each sample. we surveyed serum samples by enzyme-linked immunosorbent assay for antibodies reacting with any of a panel of 21 periodontal bacteria. the mother and 7 of her 13 children had early-onset periodontitis. bacteroides gingivalis was not detected in the subgingival flora of any affected or unaffected family member, and actin ...19854030102
sensitivity of capnocytophaga species to bactericidal properties of human serum.capnocytophaga is a newly described genus of gram-negative bacteria which can cause serious oral and extraoral infections in the susceptible host. in the present study, sensitivity of capnocytophaga spp. to the bactericidal properties of human serum was investigated. laboratory strains representative of capnocytophaga sputigena, c. ochracea, and c. gingivalis and seven oral isolates of capnocytophaga spp. obtained in primary culture were determined to be sensitive to killing by pooled normal ser ...19854044029
isolation of capnocytophaga species with a new selective medium.a selective medium (cap) composed of a gc agar base supplemented with 1% hemoglobin, 1% polyvitex, and an antibiotic mixture of polymyxin b (15 u/ml), vancomycin (5 micrograms/ml), trimethoprim (2.5 micrograms/ml), and amphotericin b (2.5 micrograms/ml) was compared with another selective medium (tbbp) and two nonselective media--a blood agar and a chocolate agar--to isolate capnocytophaga species from 725 clinical specimens. these included sputa (467 specimens), throat swabs (116 specimens), or ...19854044796
capnocytophaga ochracea: an unusual opportunistic pathogen. 19854044877
capnocytophaga species: infections in nonimmunocompromised and immunocompromised hosts. 19854045250
capnocytophaga ochracea pyonephrosis in an infant with obstructive nephropathy. 19854047964
capnocytophaga in the amniotic fluid of a woman in preterm labor with intact membranes.chorioamnionitis is thought to be uncommon with intact fetal membranes. a case is reported of a woman in preterm labor with intact membranes from whose amniotic fluid, placenta, and infant's gastric aspirate an unusual organism, capnocytophaga, was identified. the findings are discussed.19854061534
survival analysis of periodontal sites before and after periodontal therapy.periodontal diseases appear to progress with bursts of destructive activity at individual sites. one effect of treatment might be to diminish the frequency of such bursts. survival analysis was employed to seek such effects on the periodontal sites of 16 individuals with prior evidence of destructive periodontal disease. the subjects were monitored at bi-monthly intervals and actively breaking down sites were detected using attachment level measurements and the tolerance method of analysis. when ...19853860518
antibiotic susceptibility of anaerobic bacteria from the human oral cavity.anaerobic, agar-dilution, minimal inhibitory concentrations (mics) of 18 antibiotics are given for the numerically important bacterial groups from the human oral cavity. strains are divided into susceptibility categories using the guidelines for interpretation of mics suggested by the national committee for clinical laboratory standards. these guidelines are based on data on antibiotic concentrations attainable in serum following various dosage regimens. mics are also compared with attainable gi ...19853861655
lactic acid production by oral streptococcus mitis inhibits the growth of oral capnocytophaga.various relationships including inhibition or stimulation of growth have been demonstrated among the bacteria present in dental plaque, both in vitro and in vivo. a large number of these relationships involved oral streptococci. an earlier study found that strains of streptococcus mitis inhibited the growth of potential periodontopathic microorganisms, such as actinobacillus actinomycetemcomitans, capnocytophaga and species of bacteroides and fusobacterium. the present investigation showed that ...19853861843
gram negative species associated with active destructive periodontal lesions.apical subgingival plaque samples were taken from 19 subjects exhibiting active destructive periodontal disease. the predominant cultivable gram negative species from 50 active sites were compared to 69 inactive sites of comparable pocket depth and attachment level loss. active disease sites were chosen which showed a significant loss of attachment within a two-month interval. proportions of gram negative rods were higher in active periodontal disease sites than in inactive sites. species which ...19853863838
clinical and laboratory studies of a family with a high prevalence of juvenile periodontitis.the form of periodontitis with onset at puberty and affecting predominantly the first molars and incisors is called juvenile periodontitis (jp). the disease has been the object of intense study because from its analysis may come insights into understanding other, more common, forms of periodontitis. we recently had the opportunity to study an unusual family in which both parents developed jp in their teens. we did clinical examinations, measured leukocyte chemotaxis, analyzed the pocket microflo ...19853863910
[frequency of isolation of actinobacillus actinomycetemcomitans and capnocytophaga sp. in periodontitis in adults]. 19853869327
reaction of human sera from juvenile periodontitis, rapidly progressive periodontitis, and adult periodontitis patients with selected periodontopathogens.the levels of serum antibody reactive to selected periodontopathogens were determined in 182 clinically characterized patients: 35 healthy control, 50 juvenile periodontitis, 42 adult periodontitis and 55 rapidly progressive periodontitis. reactive antibody levels were determined using an enzyme-linked immunosorbent assay with whole cell preparations of bacteroides gingivalis, capnocytophaga (bacteroides) ochraceus, fusobacterium nucleatum and actinobacillus actinomycetemcomitans (y-4) serving a ...19853869650
coaggregation of oral bacteroides species with other bacteria: central role in coaggregation bridges and competitions.seventy-three freshly isolated oral strains representing 10 bacteroides spp. were tested for their ability to coaggregate with other oral gram-negative and gram-positive bacteria. none coaggregated with any of the gram-negative strains tested, which included capnocytophaga gingivalis, c. ochracea, c. sputigena, and actinobacillus actinomycetemcomitans. strains of bacteroides buccae, b. melaninogenicus, b. oralis, and b. gingivalis failed to coaggregate with any of the gram-positive strains teste ...19853888842
capnocytophaga bacteremia in a neutropenic patient with sinusitis. 19853890503
bacteriology in juvenile periodontitis. a review. 19853898064
endotoxicity of newly recognized gram negative organism: a collective review.a comprehensive picture of the in part personal investigations concerning an endotoxin-like activity associated with some recently recognized gram negative significant pathogens has been drawn. suspensions of heat-killed organisms (or endotoxic lipopolysaccharide when available) have been tested using as markers of endotoxicity the limulus assay and the skin shwartzman reaction in rabbit. some general considerations are finally set forth on the possible role of the surface structures in mediatin ...19853907664
[juvenile periodontitis. i: etiopathogenetic aspects]. 19853911054
[assessment of serum antibodies to suspected periodontal pathogens in periodontitis patients]. 19853915328
api zym and api an-ident reactions of fastidious oral gram-negative species.api zym and api an-ident enzymatic substrate tests were done on six oral species which are difficult to characterize with conventional biochemical tests. "bacteroides forsythus, the "fusiform" bacteroides species (a. c. r. tanner, m. a. listgarten, m. n. strzempko, and j. l. ebersole, manuscript in preparation), is difficult to cultivate in broth media, yet it gave 15 positive tests in these series. the tests were able to separate this new species from species of capnocytophaga and fusobacterium ...19853930558
in vitro attachment of bacteria to extracts of cementum.cementum is a specialized mineralized tissue providing for the attachment of periodontal fibers to the root surface of a tooth. in periodontal disease this connective tissue attachment to the cemental surface is lost. the ability of bacteria to adhere to the root surface, an initial event in the disease process, may be influenced by the organic matrix of cementum. therefore, an in vitro assay of cell attachment was modified to study bacterial adherence to protein extracts of cementum. petri dish ...19853932618
[assessment of serum antibodies to suspected periodontal pathogens in periodontitis patients]. 19853939719
in vitro immunosuppression mediated by an extracellular polysaccharide from capnocytophaga ochracea. influence of macrophages. 19853160840
distribution of capnocytophaga in periodontal microfloras. 19852934532
human complement activation by purified capnocytophaga exopolysaccharide. measurement by radioimmunoassay. 19862947996
the habitat of periodontopathic micro-organisms.the purpose of the present study was to investigate the significance of oral mucosal surfaces as a habitat of periodontopathic micro-organisms. for this investigation, 7 young adults were selected for an experimental gingivitis study. at the start of the experiment, bacterial samples were taken by means of a sterile swab from 4 oral mucosal surfaces: the buccal mucosa, the vestibular fold, the dorsum of the tongue and a tonsil. all individuals were asked to abstain from oral hygiene procedures i ...19863457811
[current trends in periodontology. juvenile periodontitis]. 19863462491
serum antibody levels to 4 periodontal pathogens remain unaltered after mechanical therapy of juvenile periodontitis.serum antibody titers to actinobacillus actinomycetemcomitans (a.a.) y4, bacteroides gingivalis, capnocytophaga ochracea and eubacterium saburreum were determined with an enzyme immunoassay in 12 patients with juvenile periodontitis (jp) at the beginning of treatment and 16 to 32 months later after clinically successful mechanical therapy without antibiotics. the values were compared to corresponding data obtained from 26 age- and sex-matched individuals with a healthy periodontium. 6 jp patient ...19863463569
laboratory studies of a family manifesting premature exfoliation of deciduous teeth.in a previous publication, we described the clinical and radiographic findings of a family in which the children manifested premature exfoliation of the deciduous teeth. we now report for the same family the results of extensive laboratory studies performed on blood and urine, analysis of periodontal microflora, and a family pedigree. we demonstrated the presence of putative periodontal pathogens in the subgingival microflora, elevated levels of serum antibodies reacting to bacteroides gingivali ...19863463573
antimicrobial properties of hydrogen peroxide and sodium bicarbonate individually and in combination against selected oral, gram-negative, facultative bacteria.the topical application of hydrogen peroxide (h2o2) and sodium bicarbonate (nahco3), individually and in combination, has been used empirically in the treatment of periodontal diseases. in this study, we examined both minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) of these disinfectants individually and in combination against selected facultative, gram-negative oral bacteria in a microtiter dilution assay. the bacteria studied included actinobacillus actino ...19863016051
the presence of bacteria in the oral epithelium in periodontal disease. ii. immunohistochemical identification of bacteria.serial histological sections of gingiva obtained from each of six advanced adult periodontitis, two localized juvenile periodontitis and two periodontally healthy patients were used for specific identification of bacteria within the oral epithelium and adjacent connective tissue. healthy gingival biopsies served as controls. sections from patients and control biopsies were gram-stained and also screened with antibacterial sera associated with the peroxidase immunocytochemical technique for speci ...19862427680
identification and preliminary characterization of a lectinlike protein from capnocytophaga gingivalis (emended).a polypeptide believed to be the monomeric form of the lectin responsible for the coaggregation of capnocytophaga gingivalis (emended) and actinomyces israelii has been identified. denaturing polyacrylamide gel electrophoresis and immunoblot analyses were used to distinguish the protein from other proteins in the outer membrane of c. gingivalis. the subunit of the putative lectin has a pi of 8.6 and a molecular weight of 155,000.19863943898
ability of oral bacteria to degrade fibronectin.the fibronectin-degrading ability of 116, mainly oral, strains was assayed by using plasma-derived fibronectin adsorbed to a polystyrene surface. ability to degrade fibronectin was revealed in strains of bacteroides gingivalis, bacteroides intermedius, bacteroides loeschii, staphylococcus aureus, staphylococcus epidermidis, peptococcus prevotii, clostridium sporogenes, and propionibacterium acnes. the fibronectinolytic activity of subgingival bacteriological samples was found to be related to th ...19863943910
capnocytophaga ochracea and group f beta-hemolytic streptococcus suppurative thyroiditis.the route of infection in acute suppurative thyroiditis is unknown in most cases; when demonstrated, pyriform sinus fistula appears to be the most frequent one. we report the clinical and laboratory findings of a child in whom culture of the thyroid pus yielded two bacteria which are part of the normal oropharyngeal flora: capnocytophaga ochracea and group f beta-hemolytic streptococcus. the preliminary results of the culture, which showed a mixed flora, prompted us to search and to find a pyrif ...19863959182
[isolation and identification of capnocytophaga in localized juvenile periodontitis. preliminary study]. 19863519809
gingivitis.gingivitis is caused by substances derived from microbial plaque accumulating at or near the gingival sulcus; all other suspected local and systemic etiologic factors either enhance plaque accumulation or retention, or enhance the susceptibility of the gingival tissue to microbial attack. microbial species specifically associated with gingival health include streptococcus sanguis 1, s. d-7, and fusobacterium naviforme. bacteria involved in the etiology of gingivitis include specific species of s ...19863522644
current understanding of the aetiology and progression of periodontal disease.there is overwhelming evidence that bacteria cause periodontitis and that they do so by extending apically along the surfaces of the tooth roots and creating pockets. a very complex mixture of microbial species, mostly although not exclusively gram-negative, anaerobic, and motile, is involved. infection probably occurs in a progressive and sequential manner. the bacteria involved include various species of bacteroides, actinobacillus, eikenella, fusobacterium, capnocytophaga, and eubacterium. lo ...19863533787
false results associated with darkground microscopy of subgingival plaque.this study considers false results which may arise due to problems in the preparation or examination of specimens for darkground microscopy of subgingival plaque. subgingival plaque samples obtained with a sterile curette were placed in 0.1-0.3 ml sterile full or 1/4 strength ringer's solution: 0.85% saline, 1% gelatin in 0.85% saline, formal saline or pyrogen-free water for injection. test slides were prepared from the original dispersion, and control slides from the corresponding sterile solut ...19863537016
[the prevalence and antibiotic susceptibility of capnocytophaga and actinobacillus actinomycetemcomitans in the periodontal pockets of periodontitis patients with insulin-independent diabetes mellitus]. 19863474309
arylaminopeptidase activities of oral bacteria.protease and peptidase enzymes are thought to play a role in the virulence of many oral organisms, especially those associated with periodontal diseases. in order to evaluate the peptidases of periodontopathogens, we compared the arylaminopeptidase activities of bacteroides gingivalis with those of other oral and non-oral bacteria. sixty-three bacterial strains representing the prominent cultivable organisms in human periodontal pockets were tested, including representatives of the black-pigment ...19863478393
penicillin resistance in the subgingival microbiota associated with adult periodontitis.in this investigation, the penicillin-resistant and beta-lactamase-producing subgingival microbiota associated with adult periodontitis was identified, and the impact of a recent exposure to penicillin on the recovery of resistant organisms from this microbiota was assessed. subjects with adult periodontitis were examined clinically and microbiologically. twenty-one subjects had a documented history of penicillin therapy within the previous 6 months whereas an additional 21 subjects had no histo ...19863486882
mediastinal abscess with capnocytophaga spp. in a competent host.a 51-year-old woman presented with a rapidly evolving pneumonitis complicated by the development of an anterior mediastinal abscess. upon surgical drainage, capnocytophaga spp. was the sole organism identified. this organism has been implicated in sepsis among immunocompromised hosts but has not previously been reported in this clinical setting.19863722364
capnocytophaga on the fetal surface of the placenta of a patient with ruptured membranes at 39 weeks' gestation. 19863728597
eight cases of capnocytophaga infections in denmark. 19863743561
clinical, pathogenetic, and laboratory features of capnocytophaga infections.granulocytopenia and oral mucosal defects have been reported to be important predisposing factors to recently recognized cases of capnocytophaga septicemia. the authors call attention to an apparent preponderance of these cases in the pediatric age group and emphasize laboratory features which they have found helpful in the diagnosis of capnocytophaga infections. thirteen patients with capnocytophaga infections were seen during a seven-year period. seven of these patients had capnocytophaga bact ...19863766465
multigeneric aggregations among oral bacteria: a network of independent cell-to-cell interactions.a radioactivity-based assay was developed to define the participation of radioactively labeled cell types within the milieu of unlabeled partners in multigeneric aggregates. the cell types in these multigeneric aggregations consisted of various combinations of 21 strains representing five genera of human oral bacteria. the coaggregation properties of each cell type, when paired individually with various strains, were delineated and were unchanged when the microbes took part in the more complex m ...19863782029
[capnocytophaga infections. apropos of 8 cases].eight capnocytophaga infections are described: bacteremia in immunodepressed patients (three cases), endocarditis (one case), pneumopathy (one case), buccal infection (two cases) and endometritis during use of an intrauterine contraceptive device (one case). the role of this bacterium in infections presented by immunodepressed patients is discussed in terms of literature data. identification of the genus posed no problems. species diagnosis is considered in terms of the use of conventional bioch ...19863789480
in vitro susceptibility of capnocytophaga species to 29 antimicrobial agents.a hemoglobin-supplemented medium composed of columbia agar base supplemented with 1% hemoglobin and 1% polyvitex was used to investigate the in vitro activity of 29 antimicrobial agents against capnocytophaga species. clindamycin was the most active agent, with all strains being inhibited by 0.06 microgram/ml or less. amoxicillin-clavulanic acid and imipenem were the most active among the beta-lactam antibiotics (mic for 90% of strains tested [mic90], 0.50 microgram/ml); other very active drugs ...19863800350
isolation of a strain of beta-lactamase-producing capnocytophaga ochracea. 19873494793
in vitro susceptibility of 96 capnocytophaga strains, including a beta-lactamase producer, to new beta-lactam antibiotics and six quinolones.the in vitro activities of new beta-lactam antibiotics and new quinolones were studied against 96 capnocytophaga strains, including a beta-lactamase-producing strain which was resistant to ampicillin, amoxicillin, carbenicillin, cephalothin, and cefamandole. all strains were susceptible to the combination of amoxicillin and clavulanic acid, ureidopenicillins, cefoxitin, broad-spectrum cephalosporins, and imipenem. cephalothin and cefamandole did not show good activity against most strains. all c ...19873498438
[serum antibodies of periodontally related microorganisms--changes of the igg antibodies following periodontal treatment]. 19873506006
enzymatic and antigenic characterization of immunoglobulin a1 proteases from bacteroides and capnocytophaga spp.bacteroides and capnocytophaga species have been implicated as periodontal pathogens. some of these species possess immunoglobulin a1 (iga1) proteases that are capable of cleaving the human iga1 molecule in the hinge region, leaving intact fc alpha and fab alpha fragments. the purpose of this study was to characterize this activity. in addition to iga1 protease activity in already known species, iga1 protease activity was a feature of bacteroides buccalis, bacteroides oralis, bacteroides veroral ...19873546133
in vitro susceptibility of capnocytophaga species to antimicrobial agents.a total of 33 clinical isolates of capnocytophaga spp. were susceptible to 4-quinolone antimicrobial agents. the antipseudomonal penicillins tested were equally active against all isolates, as were cefuroxime, ceftriaxone, ceftizoxime, cefotaxime, latamoxef, and ceftazidime. most isolates were resistant to trimethoprim, and some were resistant to aztreonam. most regimens for the empirical treatment of septic episodes in immunocompromised patients are suitable for the treatment of capnocytophaga ...19873566254
characterization of lectinlike surface components on capnocytophaga ochracea atcc 33596 that mediate coaggregation with gram-positive oral bacteria.the interactions between capnocytophaga ochracea atcc 33596 and streptococcus sanguis h1, actinomyces naeslundii pk984, or actinomyces israelii pk16 are dependent on specific recognitions between heat-sensitive adhesins on c. ochracea and heat-stable structures (probably carbohydrate-containing receptors) on the surfaces of these gram-positive coaggregation partners. the coaggregation of c. ochracea with each of these three organisms was inhibited by l-rhamnose and d-fucose and to a lesser exten ...19873570460
capnocytophaga species: increased resistance of clinical isolates to serum bactericidal action.capnocytophaga, a newly recognized genus of capnophilic gram-negative bacilli, is part of the normal oral flora. the capacity of capnocytophaga to cause sepsis and local infections in both immunocompromised and nonimmunocompromised hosts has been documented. given the recognition that serum resistance may contribute to the virulence of some gram-negative bacteria, we attempted to define the serum sensitivity of clinical isolates of capnocytophaga from blood and other sites of infection. whereas ...19873598230
serum and salivary antibodies in various periodontal conditions. 19873615403
improved selective culture media for actinobacillus actinomycetemcomitans and haemophilus aphrophilus.by modifying the previously described media tryptic soy-serum-bacitracin-vancomycin (tsbv) agar and tryptic soy-serum-bacitracin-vancomycin-fluoride (tsbvf) agar, two improved selective culture media were developed for isolation and enumeration of actinobacillus actinomycetemcomitans (a medium) and haemophilus aphrophilus (h medium) in oral specimens. both media were supplemented with fusidic acid and spiramycin, and carbenicillin was also added to a medium. the growth yields of pure cultures of ...19873667919
hemagglutinating activity of lipopolysaccharides from subgingival plaque bacteria.lipopolysaccharide (lps) preparations from the bacteroides species b. endodontalis, b. intermedius, b. denticola, and b. melaninogenicus and from fusobacterium nucleatum, haemophilus actinomycetemcomitans, capnocytophaga gingivalis, and eikenella corrodens directly agglutinated erythrocytes of some kinds of animals. lpss from the bacteroides species b. gingivalis, b. asaccharolyticus, b. corporis, and b. loescheii and from capnocytophaga ochracea did not possess any hemagglutinating activity. pr ...19873679548
capnocytophaga bacteremia complicating premature delivery by cesarean section.a case of endomyometritis associated with capnocytophaga ochracea bacteremia following premature delivery is described. the patient, a 30 year old woman without immunological incompetence or other predisposing disease, responded to peroral pivmecillinam. capnocytophaga spp. should be considered a possible cause of post-partum endometritis.19873436319
genomic relationships and species differentiation in the genus capnocytophaga.ten epidemiologically independent capnocytophaga strains from clinical materials constituted three distinct dna homology groups corresponding to the established species, capnocytophaga ochracea, sputigena, and gingivalis. the three groups exhibited only low, or even insignificant degrees of genomic relatedness mutually, and to a reference culture of cdc group df-2. all of five capnocytophaga ochracea strains considered were involved in topic infections whereas four strains, isolated from blood b ...19873439382
tn602: a naturally occurring relative of tn903 with direct repeats.we report the characterization of tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the r-plasmid pgd10. tn602 is highly homologous to the previously characterized tn903, present on the r-plasmid r6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-i (homologous to that of tn903) flanked by copies of an is-element homologous to is903. tn602 differs from tn903 in the following respects: the flanking is-elements (is602) are in direct ...19872819910
trypsin-like activity in subgingival plaque. a diagnostic marker for spirochetes and periodontal disease?taxonomic screening of subgingival plaque organisms with various enzyme assays have shown that treponema denticola, bacteroides gingivalis and an unspeciated capnocytophaga species possess a trypsin-like enzyme (tle) that can be detected by the hydrolysis of n-benzoyl-dl-arginine-2-naphthylamide (bana). as these organisms can be considered to be periodontopathic, it was of interest to determine whether this bana hydrolyzing enzyme could be detected directly in subgingival plaque samples. subging ...19873473222
[a case of septicemia due to capnocytophaga sputigena]. 19873119744
the effect of subgingival debridement with hand and ultrasonic instruments on the subgingival microflora.the effect of hand or ultrasonic instrumentation on the subgingival microflora of periodontal pockets was investigated. pockets with probing depths of 6-9 mm were selected in 12 patients and were randomly assigned per patient to the experimental and control groups. after oral hygiene instruction, instrumentation of the experimental pockets was carried out either by ultrasonic or by hand instruments in a split-mouth design. the treatment effect on the subgingival microbiota was evaluated by micro ...19873316297
the phospholipid components of bacteria related to periodontitis. 19873323424
rapidly progressive periodontitis. neutrophil chemotaxis inhibitory factors associated with the presence of bacteroides gingivalis in crevicular fluid.in the last few years several bacteriological and immunological studies have investigated the role of bacteria and immune defects in order to establish the etiopathogenesis of periodontal disease. with regard to the immune system, a defect in polymorphonuclear neutrophil (pmn) chemotaxis has been frequently reported in patients with rapidly progressive or juvenile periodontitis. the purpose of this study was to investigate in five patients with rapidly progressive periodontitis and normal chemot ...19873323463
[phospholipid components in some subgingival bacteria which are related to periodontitis]. 19873333375
suspected periodontopathic microorganisms and their oral habitats in young children.samples of subgingival plaque from 67 children, 5-7 years of age, were examined for the presence of certain suspected periodontal pathogenic species using the conventional technique of anaerobic sonification, dilution and spiral plating. when this technique was compared with a direct plating procedure which involved no preliminary dispersion and dilution of plaque specimens, it was found that the direct method resulted in double the frequency of children in whom black-pigmented bacteroides (bpb) ...198710870469
prevalence and microbiology of localized prepubertal periodontitis.loss of crestal alveolar bone at primary teeth was ascertained radiographically in a dental school clinical population of 2264 children. 19 patients (0.84%) demonstrated distinct periodontal bone destruction around one or more primary teeth; in only 2 of these patients had periodontal disease been identified in previous clinical examinations. a microbiological study of 35 subgingival samples from 9 available patients revealed a high prevalence of black-pigmented bacteroides spp., mainly bacteroi ...198710870470
microbiology of subgingival plaque from children with localized prepubertal periodontitis.localized prepubertal periodontitis has been described as a host-defect mediated form of bacterially induced periodontitis, with an early onset and rapid progression around a few teeth in children prior to puberty. to further our understanding of the etiology of this disease, we have examined the microbiological components of subgingival dental plaque in 9 children with localized prepubertal periodontitis to determine if patterns of putative pathogens existed, and have compared these results wit ...198710870471
characterization and cross-reactivity of rabbit antisera to plaque toxins.the effects of heat labile, high molecular weight water-soluble toxins from bacterial plaque on hl60 promyelocytic cells were examined. on gel filtration, four inhibitors of hl60 cell growth and two inhibitors of hela cell growth (pt1, pt2) were detected. the first and third hl60 cell inhibitors corresponded to the two hela cell inhibitors. the last eluted hl60 cell inhibitor (plaque leukotoxin, pl) did not inhibit hela cell growth. anti-pt2 antibodies reduced the activity of enriched pt2 by 20- ...198710870474
early microbial colonization of permucosal implants in edentulous patients.in edentulous patients, the microbial colonization of permucosal implants of sintered hydroxyapatite was studied. samples were taken from mucosa and dentures before insertion of implants and from supra- and subgingival sites two to 10 weeks after insertion. in total, five patients and 10 implants with clinically healthy peri-implant tissues were studied. the samples were investigated by dark-field microscopy and anaerobic culture. the supragingival plaque of the implants was dominated by gram-po ...198710872401
anaerobic capnocytophaga corneal ulcer. 19883358440
capnocytophaga.capnocytophaga has been found to cause disease in the normal and the immunocompromised patient. most often this organism is an opportunist that is difficult to isolate but easy to identify and a unique reminder that uncommon infection may respond to common antibiotics.19883361109
capnocytophaga species: a cause of amniotic fluid infection and preterm labour.subclinical amniotic fluid infection and subsequent preterm labour may occur with intact membranes. we report two cases of subclinical amniotic fluid infection with intact membranes presenting in preterm labour. capnocytophaga species, fastidious gram-negative bacilli normally found in oral flora, were isolated in pure culture from amniotic fluid obtained by transabdominal amniocentesis. the distinctive microbiological features and spectrum of infections associated with capnocytophaga species, a ...19883374977
necrotizing keratitis caused by capnocytophaga ochracea.we studied three cases of capnocytophaga keratitis that demonstrated stromal necrosis and a ring infiltrate. in all cases, the keratitis occurred in a previously diseased or traumatized cornea. one patient was treated with chronic antiamoebic therapy for presumed acanthamoeba keratitis. two cases resulted in corneal perforation. laboratory isolation was difficult because of slow, fastidious growth. capnocytophaga is not uniformly sensitive to commonly used topical antibiotics such as the cephalo ...19883377043
endocarditis caused by capnocytophaga ochracea.capnocytophaga ochracea is a gram-negative, fusiform bacillus which is part of the normal human oral flora. this organism is often isolated from periodontal lesions of patients with periodontitis and is associated with sepsis in granulocytopenic patients. we report here a case of endocarditis caused by c. ochracea.19883384900
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