Publications

the role of bacteroides melaninogenicus and other anaerobes in periodontal infections.	recent taxonomic and anatomical studies of dental plaque associated with periodontal health and disease have demonstrated that differences in the microbial populations in plaque may be responsible for the initiation and progression of disease. the consistent isolation of large numbers of anaerobic and capnophilic bacteria from the depths of periodontal lesions has suggested an important role for these organisms. bacteria that have been isolated include capnocytophaga (bacteroides ochraceus), oth ...	1979	44923
group and type antigens of capnocytophaga.	group-specific and type-specific antigens have been identified and purified from sonic extracts of capnocytophaga. the group-specific antigen, which was purified by affinity chromatography, was found to be identical to an antigen present in all 26 strains tested. the antigen is sensitive to trypsin, sodium dodecyl sulfate and heat labile, and composed predominantly (55%) of protein. the type-specific antigen, which was obtained by preparative immunoelectrophoresis, was found to be present in onl ...	1979	106014
capnocytophaga: new genus of gram-negative gliding bacteria. iv. dna base composition and sequence homology.		1979	117782
[aquatic myxobacteria (sporocytophaga cauliformis) and the order "myxobacterales" (author's transl)].	the indicator function of aquatic myxobacteria for the purpose of evaluating drinking water quality as well as their occurrence in sewage effluent make it desirable to describe these hitherto little known organisms in more detail. to this end, a comparative investigation of anaerobic myxobacteria of the genus sphaerocytophaga, two typical representatives of the order myxobacterales (myxococcus fulvus, sporocytophage cauliformis), and a strain ov vitrepscilla (vitroescilla proteolytica) was under ...	1979	120650
cytochromes and dehydrogenases in membranes of a new human periodontal bacterial pathogen, capnocytophaga ochracea.		1979	231958
periodontal therapy in humans. i. microbiological and clinical effects of a single course of periodontal scaling and root planing, and of adjunctive tetracycline therapy.	the present results showed that maarked and long-lasting changes in the subgingival microflora associated with periodontal disease could be achieved by a single course of periodontal treatment. immediately following therapy, the total number of subgingival organisms decreased 10- to 100-fold and the proportions of cultivable gram negative organisms and anaerobic organisms generally decreased 3- to 4-fold or more. after treatment, most periodontal pockets were populated by a scant microflora pre ...	1979	385821
septicemia due to capnocytophaga (bacteroides ocharaceus) in hodgkin's disease.		1979	434659
fatty acid composition of gliding bacteria: oral isolates of capnocytophaga compared with sporocytophaga.	the extractable and bound lipids and cellular fatty acids of the gram-negative gliding bacteria, capnocytophaga sputigena, c. gingivalis, and c. ochracea were compared to the non-host-related gliding bacterium sporocytophaga myxococcoides. the extractable lipids represented between 17 and 28% of the cell dry weight, whereas only 2 to 4% of the lipids were in the bound fraction. the methyl esters of the cellular fatty acids were mainly ac15:0, which accounted for 69 to 73% of the total extractabl ...	1979	500207
phospholipid composition of gliding bacteria: oral isolates of capnocytophaga compared with sporocytophaga.	the distribution of acetone-soluble (neutral glycolipid) and acetone-insoluble (phospholipid isoprenoids) lipids in oral isolates of gram-negative gliding bacteria of the genus capnocytophaga was compared with those in a non-host-related gliding bacterium, sporocytophaga myxococcoides. the acetone-soluble material accounted for 34 to 55% of the extracted lipids; the remainder was acetone-insoluble material. the major phospholipid was phosphatidylethanolamine (67%), with lesser amounts of lysopho ...	1979	500209
capnocytophaga: new genus of gram-negative gliding bacteria. ii. morphology and ultrastructure.	gram-negative, anaerobic gliding bacteria were isolated from normal supragingival plaque and from periodontal lesions. isolates could be divided into two size classes: small 2.4-4.2 micrograms x 0.38-0.5 microgram and large 4.8-5.8 micrograms x 0.42-0.6 microgram cells. the outer membrane was either loose-fitting and wavy, or taut, and of variable thickness. an electron-dense fuzz was discernible on several of the isolates. the periplasmic region was of variable electron-density. the genus capno ...	1979	518235
capnocytophaga: new genus of gram-negative gliding bacteria. iii. physiological characterization.	sixty-eight strains of capnophilic fusiform gram-negative rods from the human oral cavity were subjected to extensive physiologic characterization, tested for susceptibility to various antibiotics, and the mol-percent guanine plus cytosine of each isolate determined. the characteristics of the isolates were compared with 10 fresh and 2 stock isolates of fusobacterium nucleatum. the isolates clearly differed from the fusobacterium species on the basis of mol-percent guanine plus cytosine, end pro ...	1979	518236
capnocytophaga: new genus of gram-negative gliding bacteria. i. general characteristics, taxonomic considerations and significance.	the characteristics of gliding bacteria isolated from both healthy and diseased sites in the oral cavity are, summarized and the taxonomic position of the bacteria discussed. uniform attirubtes of the fusiform isolates include gliding motility, strictly fermentative metabolism dependent on the presence of co2 (or hco3-), under either anaerobic or aerobic conditions, presence of benzidine-reactive components, and the production of acetic and succinic acids as the major or sole, acidic, metabolic ...	1979	518239
ultrastructure of gliding bacteria: scanning electron microscopy of capnocytophaga sputigena, capnocytophaga gingivalis, and capnocytophaga ochracea.	when examined by both light and scanning electron microscopy, capnocytophaga gingivalis, c. sputigena, and c. ochracea displayed three distinct growth zones: the original streak, an intermediate zone, and the advancing edge, or halo zone. on trypticase (bbl microbiology systems)-soy-blood agar, the cells translocated by gliding. c. gingivalis and c. sputigena formed large, irregular isolated colonies, while c. ochracea formed a more confluent cell mass. the cells within the streak zone and in mo ...	1979	528052
synonymy of strains of center for disease control group df-1 with species of capnocytophaga.	of eight strains of center for disease control group df-1 examined, seven had 62 to 87% deoxyribonucleic acid homology with the neotype strain of capnocytophaga ochracea and one had 72% deoxyribonucleic acid homology with the type strain of c. gingivalis. deoxyribonucleic acid homology of four strains of bacteroides ochraceus with the neotype strain of c. ochrecea was 76 to 86%.	1979	528685
detection, identification, and comparison of capnocytophaga, bacteroides ochraceus, and df-1.	working independently, three laboratories had recognized considerable similarity among certain strains of dysgonic, fermentative, capnophilic, surface translocating, gram-negative bacilli referred to as capnocytophaga, bacteroides ochraceus, and center for disease control biogroup df-1. to determine the relationship among these groups, 21 strains were exchanged and independently characterized by the three laboratories. additionally, a fourth laboratory examined the deoxyribonucleic acid homologi ...	1979	528686
a neutrophil disorder induced by capnocytophaga, a dental micro-organism.	we recovered capnocytophaga, a gram-negative anaerobe implicated in the pathogenesis of periodontal disease, from two patients with a history of dental infections. neutrophils from both patients failed to acquire the asymmetric shape characteristic of normal neutrophils. fluorescein staining of the patients' living neutrophils remained diffuse and patchy instead of showing the normal pattern in which the fluorescence is swept into the rear of the cell. the locomotion of one patient's neutrophils ...	1979	481526
capnocytophaga sepsis: a newly recognised clinical entity in granulocytopenic patients.	10 episodes of capnocytophaga sepsis occurred in 6 patients with granulocytopenia, malignancy, and oral mucosal ulcerations. capnyocytophaga is a newly described genus of gliding gram-negative bacilli which is physiologically and genetically identical to bacteroides ochraceus and biogroup df-1 of the united states center for disease control and is commonly responsible for periodontal infections.	1980	6102287
capnocytophaga spp. contain sulfonolipids that are novel in procaryotes.	a group of unusual sulfonolipids was found in bacteria of the genus capnocytophaga. one of these lipids, to which we have assigned the trivial name capnine, was isolated in 98% pure form and was identified, by infrared absorption spectrometry, high-resolution mass spectrometry, and other methods, as 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid. another lipid appears to be an n-acylated version of capnine; after acid hydrolysis, its sulfur was recovered in a form chromatographically indi ...	1980	6253439
carbon dioxide metabolism by capnocytophaga ochracea: identification, characterization, and regulation of a phosphoenolpyruvate carboxykinase.	cell suspensions of capnocytophaga ochracea incorporated [14c]nahco3 into a major four-carbon fermentation product, succinate, and cell-free extracts from this organism contained high levels of phosphoenolpyruvate carboxykinase (pepck). pepck is the major, if not the only, co2(hco-3)-fixing enzyme in c. ochracea since cell-free extracts were devoid of pyruvate-dependent and other phosphoenolpyruvate (pep)-dependent co2(hco-3)-fixing enzymes. the reaction products of the enzyme, which was partial ...	1980	6769807
biological and chemical characterization of endotoxin from capnocytophaga sputigena.	an endotoxin was isolated from capnocytophaga sputigena strain 4 by a modification of the hot phenol-water method. the extraction procedure yielded a lipopolysaccharide which accounted for approximately 1.5% of the dry weight of the cells. the material was composed of 18.6% lipid (as c(15) fatty acid), 46.5% neutral sugar including 9.6% hexose, 18.3% 6-deoxy sugar, 1.0% 2-keto-3-deoxy sugar, and 4.8% heptose. hexosamine, protein, and phosphorus were found in quantities amounting to 9.0, 2.9, and ...	1980	7358428
detection of a fibroblast proliferation inhibitory factor from capnocytophaga sputigena.	the addition of a sonic extract of capnocytophaga sputigena to the culture fluid to human fibroblasts resulted in an inhibition of cell proliferation. the inhibition was dose-related (200 micrograms/ml caused a 90% inhibition, and 1,000 micrograms/ml caused a complete cessation of growth). the growth inhibition was not due to alterations in culture medium, ph or ionic strength, or to the effects of the c. sputigena lipopolysaccharide.	1980	7358430
capnocytophaga septicemia.		1980	7360185
septicemia caused by capnocytophaga in a granulocytopenic patient with glossitis.	a patient receiving immunosuppressive treatment for multiple myeloma became granulocytopenic, and acute glossitis developed. blood cultures were positive for capnocytophaga, a fastidious gram-negative bacillus that is known to be part of the normal oral flora and a pathogen for periodontitis. the infection responded to treatment with antibiotics, including penicillin g, to which the organism was sensitive. this is one of the first reports of capnocytophaga septicemia, and suggests that this orga ...	1980	7387286
modulation of immunoreactivity to periodontal disease-associated microorganisms during pregnancy.	the lymphocyte blastogenic response to a panel of antigens and mitogens was assessed in a group of 20 women throughout their pregnancy. in addition, a group of five nonpregnant women was monitored simultaneously to identify variations in response to the same stimulants. the stimulants included orally associated bacterial antigens (streptococcus sanguis, actinomyces viscosus, bacteroides asaccharolyticus, bacteroides melaninogenicus subsp. intermedius, bacteroides [capnocytophaga] ochraceus, and ...	1980	7399691
energy metabolism in capnocytophaga ochracea.	among the microflora of the gingival sulcus are members of the genus capnocytophaga which have been implicated as possible etiological agents of juvenile periodontitis and systemic infectious diseases. in this study, the pathway used by c. ochracea strain 25 for generating energy from glucose was investigated. when grown in a complex medium supplemented with glucose and nahco(3), the major end products formed were acetate (4.6 mmol), succinate (11.0 mmol), pyruvate (4.3 mmol), and oxalacetate (3 ...	1980	7216425
antimicrobial susceptibility of capnocytophaga.	capnocytophaga (bacteroides ochraceus, center for disease control biogroup df-1) is associated with sepsis in granulocytopenic patients and is isolated in large numbers from the affected periodontal pockets in patients with juvenile periodontosis. the minimal inhibitory concentrations (mics) and minimal bactericidal concentrations (mbcs) of 17 antimicrobial agents for 13 strains of capnocytophaga organisms were determined. in addition, the ratio of the mbc to the mic for each antimicrobial agent ...	1981	7247353
in vitro susceptibility of capnocytophaga strains to 18 antimicrobial agents.	twenty-seven strains of capnocytophaga were tested for their susceptibility to 18 antimicrobial agents by an agar dilution technique. all strains were susceptible to achievable blood levels of penicillin g, cefaclor, cefoxitin, cefoperazone, moxalactam, clindamycin, chloramphenicol, and tetracycline. most were susceptible to achievable levels of cefamandole, erythromycin, and metronidazole, and more than 10% were resistant to achievable levels of cephalexin and cephradine. with antimicrobial age ...	1981	7283424
[septicemia caused by capnocytophaga sputigena].		1981	7290958
polyclonal b-cell activation induced by extracts of gram-negative bacteria isolated from periodontally diseased sites.	the objective of this research was to determine whether gram-negative bacteria frequently isolated from periodontally diseased sites contained polyclonal b-cell activators. polyclonal b-cell activation, which results in nonspecific activation of multiple b-cell clones was analyzed by a hemolysis-in-gel assay designed to detect a broad range of antibody specificities. extracts from numerous bacterial strains, including bacteroides gingivalis, bacteroides melaninogenicus subsp. melaninogenicus, b. ...	1981	6975240
enzymatic characterization of some oral and nonoral gram-negative bacteria with the api zym system.	the api zym system (analytab products, plainview, n.y.), containing 19 chromogenic substrates, was utilized semiquantitatively to detect extracellular acid and alkaline phosphatases, aminopeptidases, proteases, esterase-lipase, phosphoamidase, and glycosidases in 128 oral and nonoral isolates of black-pigmented bacteroides, actinobacillus, haemophilus aphrophilus, capnocytophaga, fusobacterium nucleatum, wolinella recta, and veillonella parvula. in the black-pigmented bacteroides group of organi ...	1981	7026598
[endotoxin-like activity in capnocytophaga gingivalis].		1981	7035860
degradation of immunoglobulins a2, a2, and g by suspected principal periodontal pathogens.	attention has recently been focused on immunoglobulin a1 (iga1) protease production as a possible virulence factor of bacteria implicated in meningitis and gonorrhea. this report demonstrates that suspected principal etiological agents in destructive periodontal disease include bacteria capable of degrading iga1, iga2, and igg. representative strains of bacteroides melaninogenicus subsp. melaninogenicus and capnocytophaga cleaved iga1 but not iga2 in the hinge region to yield intact fab and fc f ...	1981	7037640
capnocytophaga ochracea septicemia.	a case report describing capnocytophaga ochracea (bacteroides ochraceus) septicemia in a 21-year-old male patient receiving chemotherapy for acute lymphocytic leukemia is presented. the unusual features of this organism are discussed together with a review of the literature.	1981	6939685
[oral gliding bacteria--capnocytophaga].		1981	6942071
ultrasonic dispersion of pure cultures of plaque bacteria and plaque.	this study compared the sonic sensitivity of 12 gram-negative and two gram-positive bacteria commonly encountered in plaque associated with periodontal diseases. pure bacterial cultures were grown to standard turbidity, diluted in 1/4 strength prereduced anaerobically sterilized ringer's solution, and aliquots dispersed for 0-180 s, using an mse sonic oscillator at 6 micron under 80% n2, 10% h2 and 10% co2. viable recoveries were determined on anaerobically cultured trypticase soy 5% blood agar ...	1981	6947390
immunological labeling of oral bacteria after demineralization. an ultrastructural study.		1981	6118403
effects of endogenous and exogenous inhibitors on the incorporation of labeled precursors into dna by human mononuclear cells.	the degree of responsiveness of lymphoid cells to activation by mitogens and antigens is commonly assessed in vitro by measuring radioactive dna precursor incorporation. several lines of evidence indicate that artifacts affect the results and that these measurements may not be an accurate reflection of cell activation. cultures of blastogenically activated lymphocytes contain soluble, noncytotoxic factors that inhibit the incorporation of radioactive nucleosides into dna by dividing cells withou ...	1982	6128306
isolation and partial characterization of a genus common antigen and species specific antigen of capnocytophaga.	sixteen strains of capnocytophaga were isolated from the pocket of a localized juvenile periodontitis patient. these strains were divided into four groups on the basis of morphological and physiological traits. strains from group i and group iii were identified as c. ochracea and group ii as c. sputigena. an antigen common to genus capnocytophaga was purified utilizing immunoabsorbent chromatography from lysates obtained by sodium dodecyl sulfate treatment of c. ochracea strain s1. an antigen sp ...	1982	6185710
influence of sublethal antibiotic concentrations on bacterial adherence to saliva-treated hydroxyapatite.	the influence of growth in the presence of sublethal concentrations of nine antibiotics on the ability of certain potentially odontopathic bacteria to attach to saliva-treated hydroxyapatite surfaces which mimic teeth was studied. cells of actinomyces viscosus ly7 and s2, bacteroides gingivalis 381, capnocytophaga ochraceus 6, and actinobacillus actinomycetemcomitans n27 attached in lower numbers to saliva-treated hydroxyapatite when grown in the presence of 50% of the minimum inhibitory concent ...	1982	6274799
incompatibility and host range of pgd10 from capnocytophaga ochraceus, formerly bacteroides ochraceus.		1982	6281834
aminopeptidase activity of capnocytophaga.		1982	6219207
plasmid content of some oral microorganisms isolated from subgingival plaque.	eighty-five strains of bacterial species selected from the predominant cultivable dental plaque flora of patients with different periodontal pathologies were examined for their plasmid content. microorganisms studied included: actinomyces viscosus, a. odontolyticus, bacteroides asaccharolyticus (b. gingivalis), b. melaninogenicus subspecies intermedius, and subspecies melaninogenicus, capnocytophaga ochracea (b. ochraceus), and fusobacterium nucleatum. three b. melaninogenicus isolates showed pl ...	1982	6949951
capnocytophaga found in root canal system of a nonvital tooth.		1982	6954444
inhibition of neutrophil chemotaxis by soluble bacterial products.	bacterial-neutrophil interactions may be critical determinants of virulence in periodontal diseases. this study was undertaken to examine the ability of major bacterial species from the human oral cavity to inhibit (1) peripheral blood neutrophil chemotaxis, (2) chemotactic formylmethionyl peptide binding, and (3) phagocytosis. included were cultured supernatants and sonic extracts obtained from strains of capnocytophaga, bacteroides gingivalis, fusobacterium nucleatum, bacteroides asaccharolyti ...	1982	6956714
scanning electron-microscopic observations on the in vitro colonization of human dentine by capnocytophaga gingivalis.		1982	6958369
selective medium for isolation of actinobacillus actinomycetemcomitans.	a selective medium, tsbv (tryptic soy-serum-bacitracin-vancomycin) agar, was developed for the isolation of actinobacillus actinomycetemcomitans, tsbv agar contained (per liter) 40 g of tryptic soy agar, 1 g of yeast extract, 100 ml of horse serum. 75 mg of bacitracin, and 5 mg of vancomycin. the tsbv medium suppressed most oral species and permitted significantly higher recovery of a. actinomycetemcomitans than nonselective blood agar medium. the distinct colonial morphology and positive catala ...	1982	7068837
capnocytophaga bacteremia in the compromised host.	capnocytophaga, a co2-requiring gram-negative bacillus, is a recently recognized pathogen in the immunocompromised host. the authors present two cases with granulocytopenia, oral disease and bacteremia with capnocytophage ochracea. the microbiology of this genus, its clinical association with periodontal disease, granulocytopenia and oral ulceration, and its implications for the compromised host are reviewed.	1982	7072641
[endocarditis caused by capnocytophaga ochracea].		1982	7079150
immunological activities of capnocytophaga cellular components.	whole cells of a clinical isolate (strain s-3) of the genus capnocytophaga were divided into cell envelope (ce) and cytoplasm (cp) fractions by mechanical disintegration followed by differential centrifugation, and a part of the ce fraction was further fractionated by sodium dodecyl sulfate (sds) treatment into the peptidoglycan and sds-supernatant fractions. the other part of the ce was extracted with butanol-water or hot phenol-water to isolate butanol-lipopolysaccharide and phenol-lipopolysac ...	1982	7095853
virulence factors of capnocytophaga: newer contributions.		1982	7113984
chemotaxonomy of the genus capnocytophaga (leadbetter, holt & socransky).		1982	7118751
[capnocytophaga infections].		1982	7122100
cervical abscess due to capnocytophaga ochracea.	a case of a cervical lymph node abscess in a child is reported from which a pure culture of c. ochracea was isolated. this is the first reported case of such an infection due to this organism. the patient, who was not leukopenic and did not receive antibiotics, was cured following excision.	1982	7145731
bacteriology of severe periodontitis in young adult humans.	a total of 78 bacteriological samples were taken from the supragingival tooth surface after superficial cleaning with toothpicks or from the periodontal sulci of 42 affected sites in 21 adolescents or young adults with severe generalized periodontitis. of 190 bacterial species, subspecies, or serotypes detected among 2,723 isolates, 11 species exceeded 1% of the subgingival flora and were most closely associated with the diseased sulci. eleven others were also sufficiently frequent to be suspect ...	1982	7152665
cellular fatty acids of capnocytophaga species.	the cellular fatty acid composition of 18 clinical isolates and 4 reference strains of capnocytophaga species (capnocytophaga ochracea, capnocytophaga gingivalis, and capnocytophaga sputigena) was determined by gas-liquid chromatography. the fatty acid profiles of the 22 cultures were essentially identical and were characterized by major amounts (60% or greater) of a saturated, iso-branched-chain, 15-carbon acid (13-methyl-tetradecanoate) and the presence of two relatively uncommon saturated, is ...	1982	7153332
[bacteriological examination of subgingival flora in patients with marginal periodontitis].	before and 4 weeks after a single course of local treatment, the composition of subgingival microflora of 15 patients with marginal periodontitis was determined by cultural and microscopical examination. a significant reduction in the number of gram-negative microorganisms could be achieved by this treatment. anaerobic sporeless rods like bacteroides and fusobacteria but also spirochetes, eikenella and capnocytophaga were involved in the inflammation. the number of actinomyces in the subgingival ...	1982	7180237
api zym system for identification of bacteroides spp., capnocytophaga spp., and spirochetes of oral origin.	a total of 80 oral strains of bacteroides gingivalis, b. asaccharolyticus, b. melaninogenicus subsp. intermedius, b. melaninogenicus subsp. melaninogenicus, capnocytophaga, treponema denticola, and t. vincentii were characterized with the api zym system for 19 enzyme activities. comparison of anaerobic and aerobic incubation with nine reference strains of these organisms showed no important differences. the key differential tests for black-pigmented bacteroides strains and treponemes of oral ori ...	1982	6764781
suppression of murine lymphocyte mitogen responses by exopolysaccharide from capnocytophaga ochracea.	an extracellular polysaccharide was purified from culture supernatants of capnocytophaga ochracea 25, a gram-negative bacillus associated with human periodontal disease. the extracellular polysaccharide suppressed in vitro mitogenic responses of murine splenic lymphocytes to concanavalin a and lipopolysaccharide. this suppression wad dose dependent, persisted up to 120 h, and was not caused by direct toxicity of the extracellular polysaccharide.	1983	6822434
selective recovery of oral capnocytophaga spp. with sheep blood agar containing bacitracin and polymyxin b.	on the basis of in vitro susceptibility testing of antibiotics, dyes, and other antimicrobial agents, we developed and evaluated a medium, tbbp, for the selective isolation of oral capnocytophaga spp. tbbp medium consists of 4% trypticase soy agar (bbl microbiology systems, cockeysville, md.), 5% sheep blood, 0.1% yeast extract, 50 micrograms of bacitracin per ml, and 100 micrograms of polymyxin b per ml. a total of 34 capnocytophaga stock cultures grew well on tbbp medium. except for some strep ...	1983	6833474
pyruvate dehydrogenase activity and metronidazole susceptibility in bacteroides fragilis.	pyruvate dehydrogenase activity was present and comparable (giving a colour change of 202-673 od units/min/g protein in our assay system) in two clinical strains of bacteroides fragilis exhibiting reduced sensitivity to metronidazole (mic 2-8 mg/l at 48 h) in a resistant strain (ingham's) of bact. fragilis (mic 64/mg/l) and in two sensitive strains of bact. fragilis (mic 0.5 mg/l). pyruvate dehydrogenase activity was not detected in a capnocytophaga strain (sensitive to 50 micrograms disc of met ...	1983	6874627
[studies of mixed anaerobic infections involving bacteroides gingivalis].	the infectiousness of several combinations of bacteria containing bacteroides gingivalis and other bacteria associated with periodontal diseases was evaluated by subcutaneous injection to guinea pigs. among the seven mixtures studied, only one permitted the development of an infection easily transmissible to a second guinea pig; this bacterial mixture was composed of b. gingivalis, fusobacterium nucleatum, eubacterium saburreum, and capnocytophaga ochracea (formerly bacteroides ochraceus). owing ...	1983	6883221
separation of bacterial ubiquinones by reverse-phase high-pressure liquid chromatography.	a procedure was developed for the separation of ubiquinones by high-pressure liquid chromatography on a reverse-phase c18 column. ubiquinones q6 through q14 were resolved in 20 min and were distinguished from menaquinones by comparing uv spectra at 248 and 275 nm.	1983	6885985
antimicrobial susceptibilities of bacteria associated with periodontal disease.	a total of 193 bacterial strains were tested for their susceptibilities to 14 antimicrobial agents. penicillin g was active at 2 u/ml against 98% of the oral isolates. other antibiotics with good activity were cefoperazone, moxalactam, sch 29,482, and clindamycin. metronidazole was active against more than 90% of the anaerobic bacteria and capnocytophaga but was inactive against most other microaerophilic and facultative strains.	1983	6601929
puberty gingivitis in insulin-dependent diabetic children. i. cross-sectional observations.	this cross-sectional study examined the gingivitis occurring at puberty in a population of insulin-dependent juvenile diabetics. seventy-seven children between the ages of 6 and 15 years were examined for gingivitis levels, stages of pubertal maturation and blood levels of glucose and glycosylated hemoglobin. bacterial plaque was sampled from one or more approximal tooth surfaces of every subject and cultured under anaerobic and aerobic conditions on nonselective and selective media. the total c ...	1983	6606030
current taxonomic status of gardnerella vaginalis.		1983	6607517
relationship between oxygen tension and subgingival bacterial flora in untreated human periodontal pockets.	the predominance of anaerobic bacteria in subgingival plaque samples suggests that the pocket environment is anaerobic. in the present investigation, a small oxygen tension (po2) electrode was inserted into the base of the pocket and the po2 was recorded. in addition, the plaque in these pockets was examined culturally and microscopically. the oxygen tension at the bottom of 36 pockets (5 to 10 mm in depth) ranged from 5 to 27 mmhg (1 mmhg congruent to 133.3 pa) with a mean value of 13.3 mmhg. m ...	1983	6642647
osteomyelitis caused by capnocytophaga ochracea.	a 13-year-old boy is reported with osteomyelitis of the greater trochanter caused by a facultative anaerobic organism (capnocytophaga ochracea) not known to cause infections in normal immunocompetent individuals, nor implicated in bone or joint infections even in compromised hosts. there remains a need to consider unusual organisms and to obtain accurate bacteriologic data in all cases of osteomyelitis.	1983	6655060
acid and alkaline phosphatases of capnocytophaga species. i. production and cytological localization of the enzymes.	the two hydrolytic enzymes, acid (acp; ec 3.1.3.2) and alkaline (alp; ec 3.1.3.1) phosphatase, of the three types species of capnocytophaga were examined. both enzymes were produced constitutively, with their activity highest in c. ochracea strain 25. these two degradative enzymes (approximately 10% of the total activity) were released into the growth medium during the latter stages of growth, both as soluble and membrane-bound enzymes. when grown in the presence of high concentrations of organi ...	1983	6661698
acid and alkaline phosphatases of capnocytophaga species. ii. isolation, purification, and characterization of the enzymes from capnocytophaga ochracea.	capnocytophaga ochracea acid (acp; ec 3.1.3.2) and alkaline (alp; ec 3.1.3.1) phosphatase was isolated by ribi cell disruption and purified by sodium dodecyl sulphate - polyacrylamide gel electrophoresis (sds-page.) both phosphatases eluted from sephadex g-150 consistent with molecular weights (migration) of 140 000 and 110 000. sds-page demonstrated a 72 000 and 55 000 subunit molecular migration for acp and alp, respectively. the kinetics of activity of purified acp and alp on p-nitrophenol ph ...	1983	6661699
acid and alkaline phosphatases of capnocytophaga species. iii. the relationship of the enzymes to the cell wall.	acid (acp) and alkaline phosphatase (alp) were localized by physicobiochemical techniques. greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted capnocytophaga species released only 9-28% and 11-43% of the cellular phosphatases, respectively. french pressure cell disruption was more effective in releasing the phosphatases. cell fragments were separated into cell wall, cytoplasmic membrane, and so ...	1983	6661700
[capnocytophaga sputigena in sinusitis].	the genus capnocytophaga was reported in the literature for the first time in 1979. capnocytophaga are fusiform gram-negative, slowly growing bacteria that require a high pco2. they belong to the normal buccal flora and they are reported as pathogens in infections of the periodontal region with progressive periodontitis and in septicaemia in patients with immunodeficiencies and buccal ulcerations. the present paper is the first to report capnocytophaga sputigena as a pathogen in sinusitis. the p ...	1983	6670527
capnocytophaga: a review of the literature.	capnocytophaga species are normal mouth flora but can be opportunistic pathogens causing juvenile peridontitis and bacteremia in the compromised host. indole-negative fusiforms isolated anaerobically or in the presence of increased co2 can presumptively be identified as capnocytophaga species.	1983	6367464
biological and chemical comparison of butanol- and phenol-water extracted lipopolysaccharide from capnocytophaga sputigena.		1983	6227738
unusual sulfonolipids are characteristic of the cytophaga-flexibacter group.	capnocytophaga spp. contain a group of unusual sulfonolipids, called capnoids (w. godchaux iii and e. r. leadbetter, j. bacteriol. 144:592-602, 1980). one of these lipids, capnine, is 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid; the others are, apparently, n-acylated versions of capnine. the lipids were found, in amounts ranging from 2.5 to 16 mumol of capnoid sulfur per g of cells (wet weight), in two cytophaga spp. and also in several closely related organisms: several capnocytophaga ...	1983	6298180
reaction of clinical isolates and typed strains of the family bacteroidaceae with rabbit anti-bacteroides sera examined by radial immunodiffusion.	antisera were produced which were reactive with bacteroides fragilis, b. gingivalis, or b. melaninogenicus subsp. melaninogenicus. anti-b. gingivalis serum exhibited strong reactions with strains of homologous species and reacted with only one other species, b. thetaiotaomicron. antigens shared by organisms of the b. fragilis group and b. melaninogenicus subspecies of the bacteroidaceae must be considered when serological methods are used for their identification.	1983	6136526
numerical taxonomy of some non-saccharolytic and saccharolytic bacteroides species.	various bacteroides spp. were examined by physiological tests, presence of specific enzymes, antibiotic sensitivity, menaquinone composition and a few miscellaneous tests. the data matrix containing 58 strains and 55 unit characters was examined using gower's similarity coefficients (sg) and included matching negative character states and multistate characters. the highly saccharolytic strains were separated from the less saccharolytic and non-fermentative strains at the 55% similarity level; wh ...	1983	6137470
neuraminidase activity of some oral anaerobic bacteria.		1983	6144743
effect of human leukocyte extracts and gingival exudate on periodontopathic bacteria.	the effect of leukocyte hydrolytic enzymes on periodontopathic bacteria was examined in vitro. a frozen and thawed extract of human peripheral blood leukocytes (le) and human gingival crevicular exudate (ge) were shown to be able to cause the release of 50% of the radioactivity from a leukotoxic strain of actinobacillus actinomycetemcomitans (aa y4), labeled by 14c. a nonleukotoxic strain (aa 653) was shown to be more susceptible to both le and ge, up to 68% of the total radioactivity was solubi ...	1983	6345380
ultrastructural studies of the effect of human leukocyte extracts on periodontopathic bacteria.	human gingival crevicular exudate (ge) and leukocyte extracts (le) were previously shown to be able to release the bulk of radioactivity from a variety of 14c-labeled oral bacteria. the present study demonstrates that the release of radiolabel by these enzymatic agents from two periodontopathic bacteria (actinobacillus actinomycetemcomitans strain y4 and capnocytophaga sputigena strain 4) is accompanied by both cell wall and cytoplasmatic damage. the possible role of bacterial products released ...	1983	6345381
the periodontal microflora of juvenile diabetics. culture, immunofluorescence, and serum antibody studies.	these studies demonstrate a unique constellation of organisms populating the subgingival area in periodontitis lesions of patients with juvenile or insulin-dependent diabetes mellitus (iddm). the cultivable microflora was predominated by capnocytophaga and anaerobic vibrios in the patients studied. in some patients, actinobacillus actinomycetemcomitans were also found. this distinguishes the subgingival flora of iddm patients suffering from periodontitis from that of patients with localized juve ...	1983	6350557
[association between specific serum antibodies to subgingival gram-negative bacteria and the degree of periodontal destruction].		1983	6355331
identification and characterization of the major cell envelope proteins of oral strains of actinobacillus actinomycetemcomitans.	the major cell envelope protein compositions of seven actinobacillus actinomycetemcomitans strains of human origin were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the major envelope polypeptides were homogeneous, in relation to molecular weight, in all of the strains that were examined. the characterization of the five major proteins, designated env1 through env5, in the leukotoxic strain y4 revealed that proteins env2 to -5 may reside in the outer membrane as suggest ...	1983	6401694
sodium fluoride susceptibilities of suspected periodontopathic bacteria.	utilizing a blood-based medium, we determined the minimum inhibitory concentrations (mic's) to sodium fluoride (naf) of 45 bacterial strains representing 22 oral species. bacterial susceptibility ranged from 128 micrograms/ml to 2048 micrograms/ml. of those organisms tested, actinobacillus actinomycetemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga ochracea, and actinomyces viscosus were susceptible at lower levels of naf.	1983	6574154
ammonia utilization by a proposed bacterial pathogen in human periodontal disease, capnocytophaga ochracea.	capnocytophaga ochracea strain 25 was originally isolated from a patient with severe juvenile periodontitis. an nad-specific glutamate dehydrogenase (gdh) (ec 1.4.1.2.) was found in cell-free extracts from this organism. the nadh-dependent reductive, or ammonia-assimilating activity (nadh-gdh), of the enzyme was 8-10-fold higher than its nad-dependent oxidative, or ammonia-releasing activity (nad-gdh), suggesting that the primary physiological role of the gdh is ammonia-fixing. capnocytophaga oc ...	1983	6576737
suppression of the periodontopathic microflora in localized juvenile periodontitis by systemic tetracycline.	since recent studies have implicated actinobacillus actinomycetemcomitans in the etiology of localized juvenile periodontitis, this investigation determined the effectiveness of subgingival debridement, topical betadine solution, and systemic tetracycline in suppressing subgingival a. actinomycetemcomitans and other microorganisms. a total of 20 deep periodontal pockets and 10 normal periodontal sites of 6 localized juvenile periodontitis patients was included in the study. each patient was trea ...	1983	6579058
microbiological study of localized juvenile periodontitis in panama.	the occurrence of subgingival actinobacillus actinomycetemcomitans and capnocytophaga in 12 localized juvenile periodontitis and 10 gingivitis patients from panama was determined using selective culture techniques. a actinomycetemcomitans was present in all localized juvenile periodontitis lesions studied and was, on average, recovered in hundred-fold-higher numbers from localized juvenile periodontitis lesions than from gingivitis lesions. capnocytophaga was only recovered in approximately thre ...	1983	6580425
modulation of in vitro human lymphocyte responses by an exopolysaccharide from capnocytophaga ochracea.	an exopolysaccharide (ep), purified from spent culture fluid previously inoculated with capnocytophaga ochracea strain 25, suppressed in vitro human peripheral blood lymphocyte responses to the mitogen concanavalin a. ep was suppressive when added to cultures before the mitogen, but enhanced responses when added 24 or 48 hr after the mitogen.	1983	6581194
black-pigmented bacteroides species, capnocytophaga species, and actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction.		1984	6583243
[susceptibility of potential periodontopathic bacteria to metronidazole, spiramycin and their combination].	a total of 65 bacterial strains originating mostly from subgingival plaque were tested for their susceptibilities to metronidazole, spiramycin, and their combination, ornidazole, erythromycin and tetracycline by means of an agar dilution technique. all agents were active against all anaerobic gram-negative rods. bacteroides gingivalis and fusobacterium nucleatum showed marked susceptibility to metronidazole (mic less than or equal to 0.06 microgram/ml) whereas 4-64 micrograms/ml were required to ...	1984	6584423
immunologic profile of juvenile periodontitis. i. lymphocyte blastogenesis and the autologous mixed lymphocyte response.	studies of blastogenesis of lymphocytes in culture from juvenile periodontitis (jp) patients have been inconclusive. experiments demonstrating differences in lymphocyte blastogenesis to preparations of putative periodontopathogens in jp and phytohaemagglutinin (pha) were performed. variables in the blastogenesis assay system recently reported were controlled which included using a range of cell and activator concentrations, incubation times of 3, 5 and 7 days, conical-bottomed microtest wells an ...	1984	6592318
[microbiological aspects of periodontal diseases].		1984	6595910
[introduction to immunology: prevention of periodontal diseases].		1984	6597216
[activity of serum antibody to periodontopathic bacteria following periodontal treatment].		1984	6597230
[bacterial examination in periodontal diseases].		1984	6597612
rapid identification of capnocytophaga isolated from septicemic patients.	four capnocytophaga strains from blood cultures of immunocompromised patients with malignant disease and the type strains of three capnocytophaga species were examined and compared to strains representing five other genera that are hard to differentiate from capnocytophaga. with three rapid identification methods, negative catalase and oxidase reactions and positive onpg assay, capnocytophaga was easily separated from eikenella corrodens, actinobacillus actinomycetemcomitans, cardiobacterium hom ...	1984	6468367
capnocytophaga ochracea infection: two cases and a review of the published work.	bacteria of the genus capnocytophaga are recently recognised pathogens which may cause oral disease and subsequent septicaemia in the immunocompromised host. we present two cases of infection caused by capnocytophaga ochracea; a soft tissue infection in an immunologically normal patient and an episode of septicaemia in a child with leukaemia. the microbiology, pathogenicity, and antimicrobial susceptibility of the genus capnocytophaga are reviewed.	1984	6470184
bacteriology of experimental gingivitis in children.	children are more resistant to gingivitis than are adults. to determine possible differences in their periodontal floras, an experimental gingivitis study, identical in design to one reported earlier with young adults, was conducted with four 4- to 6-year-old children. the incidence of sites that developed gingival index scores of 2 in children was less than one-third of the incidence observed in adults. the composition of the flora of each child was statistically significantly different from th ...	1984	6480100
the bone-resorbing activities in tissue culture of lipopolysaccharides from the bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis and capnocytophaga ochracea isolated from human mouths.	the activities of lipopolysaccharides (lps) were assessed by measuring the calcium release from mouse calvaria in vitro and compared to that of lps from salmonella typhimurium. stimulation of bone resorption was maximal at an lps concentration of 10 micrograms/ml and at this dose all oral lps preparations showed similar levels of activity and less than that of lps from s. typhimurium. only s. typhimurium lps and b. gingivalis lps retained bone-resorbing activity at 0.1 microgram/ml. no bone-reso ...	1984	6362631
taxonomy of anaerobes: present state of the art.	results of nucleic acid studies, which indicate relationships among strains and species more clearly than do usual phenotypic tests, have led to new bacteriologic nomenclature. some major changes in bacteroides include the recognition of three species (bacteroides melaninogenicus, bacteroides denticola, and bacteroides loescheii) formerly grouped in b. melaninogenicus subspecies melaninogenicus; two species (bacteroides intermedius with two closely related homology groups and bacteroides corpori ...	1984	6201990
sulfonolipids of gliding bacteria. structure of the n-acylaminosulfonates.	earlier (godchaux, w., and leadbetter, e. r. (1980) j. bacteriol. 144, 592-602; (1983) j. bacteriol. 153, 1238-1246) we demonstrated that an unusual class of sulfonolipids are major components of the cell envelope of gliding bacteria of the genus cytophaga and of closely related genera. one of these lipids, to which we have assigned the trivial name capnine, was purified and was shown to be 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid (which might also be named as 1-deoxy-15-methylhexad ...	1984	6321489