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[local immune responses to periodontopathic bacteria. i. relationship of the igg titers in serum and gingival crevicular fluid]. 19883060555
periodontopathic micro-organisms in the rice rat (oryzomys palustris).the rice rat spontaneously develops periodontal disease, and this process can be accelerated if the animal is placed on a high sucrose diet. as the aetiological agent(s) is unknown, this study was undertaken to determine the micro-organisms associated with bone loss. animals at weaning were placed on either a high sucrose diet or on regular rat chow and were sampled periodically for a variety of micro-organisms. bacteroides spp., spirochetes, and actinobacillus actinomycetemcomitans were not iso ...19883060702
degradation of basement membrane collagen by proteases from some anaerobic oral micro-organisms. 19883077815
identification of periodontopathic bacteria based upon their peptidase activities. 19883078100
[a case of septicemia due to capnocytophaga sputigena]. 19873119744
induction of activated lymphocyte killing by bacteria associated with periodontal disease.complex interactions occur among host defense cells during bacterial infection. bacteria and bacterial products may enhance or inhibit the effector and regulatory activity of human lymphocytes. accordingly, we tested the ability of human periodontal pathogens to activate peripheral blood lymphocytes using standard chromium-release assays to measure lymphocyte-mediated cytolysis. human adherent-cell depleted peripheral blood lymphocytes (pbl) with the addition of glutaraldehyde-fixed bacteria at ...19883130415
in vitro immunosuppression mediated by an extracellular polysaccharide from capnocytophaga ochracea. influence of macrophages. 19853160840
ocular capnocytophaga infection in an edentulous, immunocompetent host.severe microbial keratitis developed in a 74-year-old women receiving long-term corticosteroids. corneal smears revealed slender, fusiform gram-negative bacilli, but the lesion continued to worsen despite intensive antibiotic treatment. after several days, capnocytophaga sputigena was identified on culture. specific antimicrobial therapy was instituted, supplemented with corneal cryotherapy. there was gradual clinical improvement. however, a sterile corneal melt led to the late loss of the eye. ...19883168490
peripartum infections with capnocytophaga. a case report.capnocytophaga is an increasingly recognized genus of pathogens. originally associated with periodontal disease, this fastidious, gram-negative organism is now established as the cause of a wide variety of infections in both the immunocompetent and immunocompromised. we treated a woman for amnionitis and subsequent neonatal colonization. capnocytophaga should be considered one of the various organisms that can complicate the peripartum period.19883172065
the effects of periodontal therapy on serum antibody (igg) levels to plaque microorganisms.the influence of periodontal therapy on serum antibody titers to selected periodontal disease-associated microorganisms was assessed in 23 patients having chronic inflammatory periodontal disease (cipd). the immunoglobulin g (igg) titers were determined by the microelisa technique in serum samples obtained prior to treatment; following a hygienic phase which included scaling, root planing, and oral hygiene instruction; following surgical treatment; and one year and two years following hygienic p ...19883198782
the comparative cytotoxicity of periodontal bacteria.the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ...19883204481
the effect of human bile on bacteroides fragilis in health and disease.bacteroides fragilis is generally believed to be stimulated by bile. although b. fragilis is rarely found in the human duodenum, it is relatively frequent in gall bladder infections. to investigate this paradox, the growth of b. fragilis in the human bile in both health and disease was studied, and compared with the effect of bovine and porcine bile. b. fragilis was stimulated by the bovine bile and inhibited by the porcine bile. the normal human bile was either bacteriostatic or inhibitory wher ...19883205170
[isolation of capnocytophaga in human infections]. 19883244931
phospholipase a2: a possible virulence factor of capnocytophaga ochracea. 19883268744
serum immunoglobulin g antibody to periodontal bacteria. 19883271028
the effect of subgingival debridement with hand and ultrasonic instruments on the subgingival microflora.the effect of hand or ultrasonic instrumentation on the subgingival microflora of periodontal pockets was investigated. pockets with probing depths of 6-9 mm were selected in 12 patients and were randomly assigned per patient to the experimental and control groups. after oral hygiene instruction, instrumentation of the experimental pockets was carried out either by ultrasonic or by hand instruments in a split-mouth design. the treatment effect on the subgingival microbiota was evaluated by micro ...19873316297
the phospholipid components of bacteria related to periodontitis. 19873323424
rapidly progressive periodontitis. neutrophil chemotaxis inhibitory factors associated with the presence of bacteroides gingivalis in crevicular fluid.in the last few years several bacteriological and immunological studies have investigated the role of bacteria and immune defects in order to establish the etiopathogenesis of periodontal disease. with regard to the immune system, a defect in polymorphonuclear neutrophil (pmn) chemotaxis has been frequently reported in patients with rapidly progressive or juvenile periodontitis. the purpose of this study was to investigate in five patients with rapidly progressive periodontitis and normal chemot ...19873323463
[phospholipid components in some subgingival bacteria which are related to periodontitis]. 19873333375
anaerobic capnocytophaga corneal ulcer. 19883358440
capnocytophaga.capnocytophaga has been found to cause disease in the normal and the immunocompromised patient. most often this organism is an opportunist that is difficult to isolate but easy to identify and a unique reminder that uncommon infection may respond to common antibiotics.19883361109
capnocytophaga species: a cause of amniotic fluid infection and preterm labour.subclinical amniotic fluid infection and subsequent preterm labour may occur with intact membranes. we report two cases of subclinical amniotic fluid infection with intact membranes presenting in preterm labour. capnocytophaga species, fastidious gram-negative bacilli normally found in oral flora, were isolated in pure culture from amniotic fluid obtained by transabdominal amniocentesis. the distinctive microbiological features and spectrum of infections associated with capnocytophaga species, a ...19883374977
necrotizing keratitis caused by capnocytophaga ochracea.we studied three cases of capnocytophaga keratitis that demonstrated stromal necrosis and a ring infiltrate. in all cases, the keratitis occurred in a previously diseased or traumatized cornea. one patient was treated with chronic antiamoebic therapy for presumed acanthamoeba keratitis. two cases resulted in corneal perforation. laboratory isolation was difficult because of slow, fastidious growth. capnocytophaga is not uniformly sensitive to commonly used topical antibiotics such as the cephalo ...19883377043
endocarditis caused by capnocytophaga ochracea.capnocytophaga ochracea is a gram-negative, fusiform bacillus which is part of the normal human oral flora. this organism is often isolated from periodontal lesions of patients with periodontitis and is associated with sepsis in granulocytopenic patients. we report here a case of endocarditis caused by c. ochracea.19883384900
microbiological and clinical effects of chlorhexidine digluconate and hydrogen peroxide mouthrinses on developing plaque and gingivitis.while the ability of chlorhexidine (chx) to prevent plaque formation and inhibit the development of gingivitis has been well documented in the literature, the therapeutic value of hydrogen peroxide (h2o2) in preventing gingivitis is in dispute. the purpose of this study was to compare the clinical and microbiological effects of an established therapeutic agent, such as chlorhexidine with that of h2o2 in the experimental gingivitis model. following a period of stringent oral hygiene, 32 subjects ...19883422246
capnocytophaga bacteremia complicating premature delivery by cesarean section.a case of endomyometritis associated with capnocytophaga ochracea bacteremia following premature delivery is described. the patient, a 30 year old woman without immunological incompetence or other predisposing disease, responded to peroral pivmecillinam. capnocytophaga spp. should be considered a possible cause of post-partum endometritis.19873436319
genomic relationships and species differentiation in the genus capnocytophaga.ten epidemiologically independent capnocytophaga strains from clinical materials constituted three distinct dna homology groups corresponding to the established species, capnocytophaga ochracea, sputigena, and gingivalis. the three groups exhibited only low, or even insignificant degrees of genomic relatedness mutually, and to a reference culture of cdc group df-2. all of five capnocytophaga ochracea strains considered were involved in topic infections whereas four strains, isolated from blood b ...19873439382
the habitat of periodontopathic micro-organisms.the purpose of the present study was to investigate the significance of oral mucosal surfaces as a habitat of periodontopathic micro-organisms. for this investigation, 7 young adults were selected for an experimental gingivitis study. at the start of the experiment, bacterial samples were taken by means of a sterile swab from 4 oral mucosal surfaces: the buccal mucosa, the vestibular fold, the dorsum of the tongue and a tonsil. all individuals were asked to abstain from oral hygiene procedures i ...19863457811
[current trends in periodontology. juvenile periodontitis]. 19863462491
serum antibody levels to 4 periodontal pathogens remain unaltered after mechanical therapy of juvenile periodontitis.serum antibody titers to actinobacillus actinomycetemcomitans (a.a.) y4, bacteroides gingivalis, capnocytophaga ochracea and eubacterium saburreum were determined with an enzyme immunoassay in 12 patients with juvenile periodontitis (jp) at the beginning of treatment and 16 to 32 months later after clinically successful mechanical therapy without antibiotics. the values were compared to corresponding data obtained from 26 age- and sex-matched individuals with a healthy periodontium. 6 jp patient ...19863463569
laboratory studies of a family manifesting premature exfoliation of deciduous teeth.in a previous publication, we described the clinical and radiographic findings of a family in which the children manifested premature exfoliation of the deciduous teeth. we now report for the same family the results of extensive laboratory studies performed on blood and urine, analysis of periodontal microflora, and a family pedigree. we demonstrated the presence of putative periodontal pathogens in the subgingival microflora, elevated levels of serum antibodies reacting to bacteroides gingivali ...19863463573
trypsin-like activity in subgingival plaque. a diagnostic marker for spirochetes and periodontal disease?taxonomic screening of subgingival plaque organisms with various enzyme assays have shown that treponema denticola, bacteroides gingivalis and an unspeciated capnocytophaga species possess a trypsin-like enzyme (tle) that can be detected by the hydrolysis of n-benzoyl-dl-arginine-2-naphthylamide (bana). as these organisms can be considered to be periodontopathic, it was of interest to determine whether this bana hydrolyzing enzyme could be detected directly in subgingival plaque samples. subging ...19873473222
[the prevalence and antibiotic susceptibility of capnocytophaga and actinobacillus actinomycetemcomitans in the periodontal pockets of periodontitis patients with insulin-independent diabetes mellitus]. 19863474309
arylaminopeptidase activities of oral bacteria.protease and peptidase enzymes are thought to play a role in the virulence of many oral organisms, especially those associated with periodontal diseases. in order to evaluate the peptidases of periodontopathogens, we compared the arylaminopeptidase activities of bacteroides gingivalis with those of other oral and non-oral bacteria. sixty-three bacterial strains representing the prominent cultivable organisms in human periodontal pockets were tested, including representatives of the black-pigment ...19863478393
penicillin resistance in the subgingival microbiota associated with adult periodontitis.in this investigation, the penicillin-resistant and beta-lactamase-producing subgingival microbiota associated with adult periodontitis was identified, and the impact of a recent exposure to penicillin on the recovery of resistant organisms from this microbiota was assessed. subjects with adult periodontitis were examined clinically and microbiologically. twenty-one subjects had a documented history of penicillin therapy within the previous 6 months whereas an additional 21 subjects had no histo ...19863486882
isolation of a strain of beta-lactamase-producing capnocytophaga ochracea. 19873494793
in vitro susceptibility of 96 capnocytophaga strains, including a beta-lactamase producer, to new beta-lactam antibiotics and six quinolones.the in vitro activities of new beta-lactam antibiotics and new quinolones were studied against 96 capnocytophaga strains, including a beta-lactamase-producing strain which was resistant to ampicillin, amoxicillin, carbenicillin, cephalothin, and cefamandole. all strains were susceptible to the combination of amoxicillin and clavulanic acid, ureidopenicillins, cefoxitin, broad-spectrum cephalosporins, and imipenem. cephalothin and cefamandole did not show good activity against most strains. all c ...19873498438
[serum antibodies of periodontally related microorganisms--changes of the igg antibodies following periodontal treatment]. 19873506006
[isolation and identification of capnocytophaga in localized juvenile periodontitis. preliminary study]. 19863519809
gingivitis.gingivitis is caused by substances derived from microbial plaque accumulating at or near the gingival sulcus; all other suspected local and systemic etiologic factors either enhance plaque accumulation or retention, or enhance the susceptibility of the gingival tissue to microbial attack. microbial species specifically associated with gingival health include streptococcus sanguis 1, s. d-7, and fusobacterium naviforme. bacteria involved in the etiology of gingivitis include specific species of s ...19863522644
current understanding of the aetiology and progression of periodontal disease.there is overwhelming evidence that bacteria cause periodontitis and that they do so by extending apically along the surfaces of the tooth roots and creating pockets. a very complex mixture of microbial species, mostly although not exclusively gram-negative, anaerobic, and motile, is involved. infection probably occurs in a progressive and sequential manner. the bacteria involved include various species of bacteroides, actinobacillus, eikenella, fusobacterium, capnocytophaga, and eubacterium. lo ...19863533787
false results associated with darkground microscopy of subgingival plaque.this study considers false results which may arise due to problems in the preparation or examination of specimens for darkground microscopy of subgingival plaque. subgingival plaque samples obtained with a sterile curette were placed in 0.1-0.3 ml sterile full or 1/4 strength ringer's solution: 0.85% saline, 1% gelatin in 0.85% saline, formal saline or pyrogen-free water for injection. test slides were prepared from the original dispersion, and control slides from the corresponding sterile solut ...19863537016
enzymatic and antigenic characterization of immunoglobulin a1 proteases from bacteroides and capnocytophaga spp.bacteroides and capnocytophaga species have been implicated as periodontal pathogens. some of these species possess immunoglobulin a1 (iga1) proteases that are capable of cleaving the human iga1 molecule in the hinge region, leaving intact fc alpha and fab alpha fragments. the purpose of this study was to characterize this activity. in addition to iga1 protease activity in already known species, iga1 protease activity was a feature of bacteroides buccalis, bacteroides oralis, bacteroides veroral ...19873546133
in vitro susceptibility of capnocytophaga species to antimicrobial agents.a total of 33 clinical isolates of capnocytophaga spp. were susceptible to 4-quinolone antimicrobial agents. the antipseudomonal penicillins tested were equally active against all isolates, as were cefuroxime, ceftriaxone, ceftizoxime, cefotaxime, latamoxef, and ceftazidime. most isolates were resistant to trimethoprim, and some were resistant to aztreonam. most regimens for the empirical treatment of septic episodes in immunocompromised patients are suitable for the treatment of capnocytophaga ...19873566254
characterization of lectinlike surface components on capnocytophaga ochracea atcc 33596 that mediate coaggregation with gram-positive oral bacteria.the interactions between capnocytophaga ochracea atcc 33596 and streptococcus sanguis h1, actinomyces naeslundii pk984, or actinomyces israelii pk16 are dependent on specific recognitions between heat-sensitive adhesins on c. ochracea and heat-stable structures (probably carbohydrate-containing receptors) on the surfaces of these gram-positive coaggregation partners. the coaggregation of c. ochracea with each of these three organisms was inhibited by l-rhamnose and d-fucose and to a lesser exten ...19873570460
capnocytophaga species: increased resistance of clinical isolates to serum bactericidal action.capnocytophaga, a newly recognized genus of capnophilic gram-negative bacilli, is part of the normal oral flora. the capacity of capnocytophaga to cause sepsis and local infections in both immunocompromised and nonimmunocompromised hosts has been documented. given the recognition that serum resistance may contribute to the virulence of some gram-negative bacteria, we attempted to define the serum sensitivity of clinical isolates of capnocytophaga from blood and other sites of infection. whereas ...19873598230
serum and salivary antibodies in various periodontal conditions. 19873615403
improved selective culture media for actinobacillus actinomycetemcomitans and haemophilus aphrophilus.by modifying the previously described media tryptic soy-serum-bacitracin-vancomycin (tsbv) agar and tryptic soy-serum-bacitracin-vancomycin-fluoride (tsbvf) agar, two improved selective culture media were developed for isolation and enumeration of actinobacillus actinomycetemcomitans (a medium) and haemophilus aphrophilus (h medium) in oral specimens. both media were supplemented with fusidic acid and spiramycin, and carbenicillin was also added to a medium. the growth yields of pure cultures of ...19873667919
hemagglutinating activity of lipopolysaccharides from subgingival plaque bacteria.lipopolysaccharide (lps) preparations from the bacteroides species b. endodontalis, b. intermedius, b. denticola, and b. melaninogenicus and from fusobacterium nucleatum, haemophilus actinomycetemcomitans, capnocytophaga gingivalis, and eikenella corrodens directly agglutinated erythrocytes of some kinds of animals. lpss from the bacteroides species b. gingivalis, b. asaccharolyticus, b. corporis, and b. loescheii and from capnocytophaga ochracea did not possess any hemagglutinating activity. pr ...19873679548
mediastinal abscess with capnocytophaga spp. in a competent host.a 51-year-old woman presented with a rapidly evolving pneumonitis complicated by the development of an anterior mediastinal abscess. upon surgical drainage, capnocytophaga spp. was the sole organism identified. this organism has been implicated in sepsis among immunocompromised hosts but has not previously been reported in this clinical setting.19863722364
capnocytophaga on the fetal surface of the placenta of a patient with ruptured membranes at 39 weeks' gestation. 19863728597
eight cases of capnocytophaga infections in denmark. 19863743561
clinical, pathogenetic, and laboratory features of capnocytophaga infections.granulocytopenia and oral mucosal defects have been reported to be important predisposing factors to recently recognized cases of capnocytophaga septicemia. the authors call attention to an apparent preponderance of these cases in the pediatric age group and emphasize laboratory features which they have found helpful in the diagnosis of capnocytophaga infections. thirteen patients with capnocytophaga infections were seen during a seven-year period. seven of these patients had capnocytophaga bact ...19863766465
multigeneric aggregations among oral bacteria: a network of independent cell-to-cell interactions.a radioactivity-based assay was developed to define the participation of radioactively labeled cell types within the milieu of unlabeled partners in multigeneric aggregates. the cell types in these multigeneric aggregations consisted of various combinations of 21 strains representing five genera of human oral bacteria. the coaggregation properties of each cell type, when paired individually with various strains, were delineated and were unchanged when the microbes took part in the more complex m ...19863782029
[capnocytophaga infections. apropos of 8 cases].eight capnocytophaga infections are described: bacteremia in immunodepressed patients (three cases), endocarditis (one case), pneumopathy (one case), buccal infection (two cases) and endometritis during use of an intrauterine contraceptive device (one case). the role of this bacterium in infections presented by immunodepressed patients is discussed in terms of literature data. identification of the genus posed no problems. species diagnosis is considered in terms of the use of conventional bioch ...19863789480
in vitro susceptibility of capnocytophaga species to 29 antimicrobial agents.a hemoglobin-supplemented medium composed of columbia agar base supplemented with 1% hemoglobin and 1% polyvitex was used to investigate the in vitro activity of 29 antimicrobial agents against capnocytophaga species. clindamycin was the most active agent, with all strains being inhibited by 0.06 microgram/ml or less. amoxicillin-clavulanic acid and imipenem were the most active among the beta-lactam antibiotics (mic for 90% of strains tested [mic90], 0.50 microgram/ml); other very active drugs ...19863800350
survival analysis of periodontal sites before and after periodontal therapy.periodontal diseases appear to progress with bursts of destructive activity at individual sites. one effect of treatment might be to diminish the frequency of such bursts. survival analysis was employed to seek such effects on the periodontal sites of 16 individuals with prior evidence of destructive periodontal disease. the subjects were monitored at bi-monthly intervals and actively breaking down sites were detected using attachment level measurements and the tolerance method of analysis. when ...19853860518
antibiotic susceptibility of anaerobic bacteria from the human oral cavity.anaerobic, agar-dilution, minimal inhibitory concentrations (mics) of 18 antibiotics are given for the numerically important bacterial groups from the human oral cavity. strains are divided into susceptibility categories using the guidelines for interpretation of mics suggested by the national committee for clinical laboratory standards. these guidelines are based on data on antibiotic concentrations attainable in serum following various dosage regimens. mics are also compared with attainable gi ...19853861655
lactic acid production by oral streptococcus mitis inhibits the growth of oral capnocytophaga.various relationships including inhibition or stimulation of growth have been demonstrated among the bacteria present in dental plaque, both in vitro and in vivo. a large number of these relationships involved oral streptococci. an earlier study found that strains of streptococcus mitis inhibited the growth of potential periodontopathic microorganisms, such as actinobacillus actinomycetemcomitans, capnocytophaga and species of bacteroides and fusobacterium. the present investigation showed that ...19853861843
gram negative species associated with active destructive periodontal lesions.apical subgingival plaque samples were taken from 19 subjects exhibiting active destructive periodontal disease. the predominant cultivable gram negative species from 50 active sites were compared to 69 inactive sites of comparable pocket depth and attachment level loss. active disease sites were chosen which showed a significant loss of attachment within a two-month interval. proportions of gram negative rods were higher in active periodontal disease sites than in inactive sites. species which ...19853863838
clinical and laboratory studies of a family with a high prevalence of juvenile periodontitis.the form of periodontitis with onset at puberty and affecting predominantly the first molars and incisors is called juvenile periodontitis (jp). the disease has been the object of intense study because from its analysis may come insights into understanding other, more common, forms of periodontitis. we recently had the opportunity to study an unusual family in which both parents developed jp in their teens. we did clinical examinations, measured leukocyte chemotaxis, analyzed the pocket microflo ...19853863910
[frequency of isolation of actinobacillus actinomycetemcomitans and capnocytophaga sp. in periodontitis in adults]. 19853869327
reaction of human sera from juvenile periodontitis, rapidly progressive periodontitis, and adult periodontitis patients with selected periodontopathogens.the levels of serum antibody reactive to selected periodontopathogens were determined in 182 clinically characterized patients: 35 healthy control, 50 juvenile periodontitis, 42 adult periodontitis and 55 rapidly progressive periodontitis. reactive antibody levels were determined using an enzyme-linked immunosorbent assay with whole cell preparations of bacteroides gingivalis, capnocytophaga (bacteroides) ochraceus, fusobacterium nucleatum and actinobacillus actinomycetemcomitans (y-4) serving a ...19853869650
coaggregation of oral bacteroides species with other bacteria: central role in coaggregation bridges and competitions.seventy-three freshly isolated oral strains representing 10 bacteroides spp. were tested for their ability to coaggregate with other oral gram-negative and gram-positive bacteria. none coaggregated with any of the gram-negative strains tested, which included capnocytophaga gingivalis, c. ochracea, c. sputigena, and actinobacillus actinomycetemcomitans. strains of bacteroides buccae, b. melaninogenicus, b. oralis, and b. gingivalis failed to coaggregate with any of the gram-positive strains teste ...19853888842
capnocytophaga bacteremia in a neutropenic patient with sinusitis. 19853890503
bacteriology in juvenile periodontitis. a review. 19853898064
endotoxicity of newly recognized gram negative organism: a collective review.a comprehensive picture of the in part personal investigations concerning an endotoxin-like activity associated with some recently recognized gram negative significant pathogens has been drawn. suspensions of heat-killed organisms (or endotoxic lipopolysaccharide when available) have been tested using as markers of endotoxicity the limulus assay and the skin shwartzman reaction in rabbit. some general considerations are finally set forth on the possible role of the surface structures in mediatin ...19853907664
[juvenile periodontitis. i: etiopathogenetic aspects]. 19853911054
[assessment of serum antibodies to suspected periodontal pathogens in periodontitis patients]. 19853915328
api zym and api an-ident reactions of fastidious oral gram-negative species.api zym and api an-ident enzymatic substrate tests were done on six oral species which are difficult to characterize with conventional biochemical tests. "bacteroides forsythus, the "fusiform" bacteroides species (a. c. r. tanner, m. a. listgarten, m. n. strzempko, and j. l. ebersole, manuscript in preparation), is difficult to cultivate in broth media, yet it gave 15 positive tests in these series. the tests were able to separate this new species from species of capnocytophaga and fusobacterium ...19853930558
in vitro attachment of bacteria to extracts of cementum.cementum is a specialized mineralized tissue providing for the attachment of periodontal fibers to the root surface of a tooth. in periodontal disease this connective tissue attachment to the cemental surface is lost. the ability of bacteria to adhere to the root surface, an initial event in the disease process, may be influenced by the organic matrix of cementum. therefore, an in vitro assay of cell attachment was modified to study bacterial adherence to protein extracts of cementum. petri dish ...19853932618
[assessment of serum antibodies to suspected periodontal pathogens in periodontitis patients]. 19853939719
identification and preliminary characterization of a lectinlike protein from capnocytophaga gingivalis (emended).a polypeptide believed to be the monomeric form of the lectin responsible for the coaggregation of capnocytophaga gingivalis (emended) and actinomyces israelii has been identified. denaturing polyacrylamide gel electrophoresis and immunoblot analyses were used to distinguish the protein from other proteins in the outer membrane of c. gingivalis. the subunit of the putative lectin has a pi of 8.6 and a molecular weight of 155,000.19863943898
ability of oral bacteria to degrade fibronectin.the fibronectin-degrading ability of 116, mainly oral, strains was assayed by using plasma-derived fibronectin adsorbed to a polystyrene surface. ability to degrade fibronectin was revealed in strains of bacteroides gingivalis, bacteroides intermedius, bacteroides loeschii, staphylococcus aureus, staphylococcus epidermidis, peptococcus prevotii, clostridium sporogenes, and propionibacterium acnes. the fibronectinolytic activity of subgingival bacteriological samples was found to be related to th ...19863943910
capnocytophaga ochracea and group f beta-hemolytic streptococcus suppurative thyroiditis.the route of infection in acute suppurative thyroiditis is unknown in most cases; when demonstrated, pyriform sinus fistula appears to be the most frequent one. we report the clinical and laboratory findings of a child in whom culture of the thyroid pus yielded two bacteria which are part of the normal oropharyngeal flora: capnocytophaga ochracea and group f beta-hemolytic streptococcus. the preliminary results of the culture, which showed a mixed flora, prompted us to search and to find a pyrif ...19863959182
capnocytophaga species: infections in nonimmunocompromised and immunocompromised hosts.retrospective review of isolates of capnocytophaga, a genus of capnophilic gram-negative bacilli, referred to the massachusetts state laboratory institute in boston revealed 31 patients with infection due to capnocytophaga, 16 in nonimmunocompromised hosts. these infections included empyema (three patients), lung abscess (one), sinusitis (one), conjunctivitis (three), subphrenic abscess (one), wound (three), osteomyelitis (one), and bacteremia (three). two of the wound infections were closed-fis ...19853965585
lysozyme-mediated aggregation and lysis of the periodontal microorganism capnocytophaga gingivalis 2010.the ability of lysozyme to aggregate and lyse the gram-negative capnophilic periodontal microorganism capnocytophaga gingivalis 2010 was monitored optically at 540 nm. both hen egg white and chromatographically purified human lysozymes had significant but similar aggregation potentials for both logarithmic- and stationary-phase bacteria. in general, an increase in enzyme concentration resulted in a graded increase in both the initial and maximum changes in turbidity which occurred during the rea ...19853967924
degradation of human immunoglobulins g and m and complement factors c3 and c5 by black-pigmented bacteroides.strains of bacteroides, capnocytophaga and fusobacterium were examined by immunological methods for their ability to degrade the human serum proteins igg, igm, c3 and c5. the proteolytic activity of the strains was measured in terms of the breakdown of serum into trichloroacetic acid-soluble material. only black-pigmented bacteroides strains showed proteolytic activity. strains of b. gingivalis degraded igg, igm, c3 and c5, strains of b. intermedius igg and c3, strains of b. endodontalis c3 and ...19853968707
capnocytophaga isolated from the endometrium as a cause of neonatal sepsis. a case report.capnocytophaga, a genus of gram-negative, anaerobic organisms, was isolated from the endometrium of a postpartum woman and the blood of the neonate. this unusual organism, usually isolated from the oral cavity, is sensitive to most antibiotics except the aminoglycosides.19853973864
capnocytophaga keratitis.we report two cases of bacterial keratitis caused by capnocytophaga, a genus of capnophilic gram negative bacilli. both responded to topical and subconjunctival clindamycin.19853978064
purification and chemical characterization of an exopolysaccharide isolated from capnocytophaga ochracea.purification and chemical characterization of an immunosuppressive exopolysaccharide from capnocytophaga ochracea strain 25 are described. this polysaccharide was extracted from spent culture medium by cold ethanol precipitation. purification was accomplished by trichloroacetic acid and pronase treatments in combination with diethylaminoethyl-sepharose and concanavalin a-sepharose chromatography. purity of the exopolysaccharide was ascertained by polyacrylamide gel electrophoresis using periodic ...19853986710
comparative bacteriology of juvenile periodontitis.statistical comparisons of the floras associated with juvenile periodontitis, severe periodontitis, and moderate periodontitis indicated that differences in the bacterial compositions of affected sites in these populations were not statistically significant. the subgingival flora of affected juvenile periodontitis sites was statistically significantly different from the adjacent supragingival flora and from the subgingival floras of people with healthy gingiva and of children with developing (ex ...19853988344
isolation of neisseria elongata and of capnocytophaga ochracea from vaginal specimens. 19854008966
capnocytophaga: a pathogen in immunocompetent hosts. 19854008992
induction of spheroplasts in capnocytophaga ochracea.a gentle technique for preparing spheroplasts of capnocytophaga ochracea strain 25 is described. cells in the exponential phase were washed with 1.0 m-nacl, agitated in 1.0 m-nacl for 2 h at 30 degrees c and exposed to lysozyme in a tris/salts buffer, ph 7.0. this procedure resulted in 98% spheroplast formation with complete removal of the peptidoglycan layer as detected by both phase-contrast and electron microscopy in combination with chemical analysis.19854020345
congenital bacteremia due to capnocytophaga. 19854022809
peritonitis involving capnocytophaga ochracea.spontaneous bacterial peritonitis, due to capnocytophaga ochracea, developed in a 65-year-old alcoholic patient with extensive cirrhosis and ascites. previously reported human infections with this organism have included peridontal diseases, septicemia, and arthritis. this is the first report of spontaneous bacterial peritonitis associated with capnocytophaga species.19854028664
assessment of serum antibody patterns and analysis of subgingival microflora of members of a family with a high prevalence of early-onset periodontitis.in a study of members of a large family with a high prevalence of early-onset periodontitis, we sampled the subgingival microflora and characterized 40 isolates from each sample. we surveyed serum samples by enzyme-linked immunosorbent assay for antibodies reacting with any of a panel of 21 periodontal bacteria. the mother and 7 of her 13 children had early-onset periodontitis. bacteroides gingivalis was not detected in the subgingival flora of any affected or unaffected family member, and actin ...19854030102
sensitivity of capnocytophaga species to bactericidal properties of human serum.capnocytophaga is a newly described genus of gram-negative bacteria which can cause serious oral and extraoral infections in the susceptible host. in the present study, sensitivity of capnocytophaga spp. to the bactericidal properties of human serum was investigated. laboratory strains representative of capnocytophaga sputigena, c. ochracea, and c. gingivalis and seven oral isolates of capnocytophaga spp. obtained in primary culture were determined to be sensitive to killing by pooled normal ser ...19854044029
isolation of capnocytophaga species with a new selective medium.a selective medium (cap) composed of a gc agar base supplemented with 1% hemoglobin, 1% polyvitex, and an antibiotic mixture of polymyxin b (15 u/ml), vancomycin (5 micrograms/ml), trimethoprim (2.5 micrograms/ml), and amphotericin b (2.5 micrograms/ml) was compared with another selective medium (tbbp) and two nonselective media--a blood agar and a chocolate agar--to isolate capnocytophaga species from 725 clinical specimens. these included sputa (467 specimens), throat swabs (116 specimens), or ...19854044796
capnocytophaga ochracea: an unusual opportunistic pathogen. 19854044877
capnocytophaga species: infections in nonimmunocompromised and immunocompromised hosts. 19854045250
capnocytophaga ochracea pyonephrosis in an infant with obstructive nephropathy. 19854047964
capnocytophaga in the amniotic fluid of a woman in preterm labor with intact membranes.chorioamnionitis is thought to be uncommon with intact fetal membranes. a case is reported of a woman in preterm labor with intact membranes from whose amniotic fluid, placenta, and infant's gastric aspirate an unusual organism, capnocytophaga, was identified. the findings are discussed.19854061534
capnocytophaga sepsis: a newly recognised clinical entity in granulocytopenic patients.10 episodes of capnocytophaga sepsis occurred in 6 patients with granulocytopenia, malignancy, and oral mucosal ulcerations. capnyocytophaga is a newly described genus of gliding gram-negative bacilli which is physiologically and genetically identical to bacteroides ochraceus and biogroup df-1 of the united states center for disease control and is commonly responsible for periodontal infections.19806102287
immunological labeling of oral bacteria after demineralization. an ultrastructural study. 19816118403
effects of endogenous and exogenous inhibitors on the incorporation of labeled precursors into dna by human mononuclear cells.the degree of responsiveness of lymphoid cells to activation by mitogens and antigens is commonly assessed in vitro by measuring radioactive dna precursor incorporation. several lines of evidence indicate that artifacts affect the results and that these measurements may not be an accurate reflection of cell activation. cultures of blastogenically activated lymphocytes contain soluble, noncytotoxic factors that inhibit the incorporation of radioactive nucleosides into dna by dividing cells withou ...19826128306
reaction of clinical isolates and typed strains of the family bacteroidaceae with rabbit anti-bacteroides sera examined by radial immunodiffusion.antisera were produced which were reactive with bacteroides fragilis, b. gingivalis, or b. melaninogenicus subsp. melaninogenicus. anti-b. gingivalis serum exhibited strong reactions with strains of homologous species and reacted with only one other species, b. thetaiotaomicron. antigens shared by organisms of the b. fragilis group and b. melaninogenicus subspecies of the bacteroidaceae must be considered when serological methods are used for their identification.19836136526
numerical taxonomy of some non-saccharolytic and saccharolytic bacteroides species.various bacteroides spp. were examined by physiological tests, presence of specific enzymes, antibiotic sensitivity, menaquinone composition and a few miscellaneous tests. the data matrix containing 58 strains and 55 unit characters was examined using gower's similarity coefficients (sg) and included matching negative character states and multistate characters. the highly saccharolytic strains were separated from the less saccharolytic and non-fermentative strains at the 55% similarity level; wh ...19836137470
neuraminidase activity of some oral anaerobic bacteria. 19836144743
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