| induced structural transitions in trna. | | 1971 | 248323 |
| [concentration of serum iron and transferrin saturation with iron as indices of changes resulting from chronic exposure to chemicals in industry]. | an attempt was undertaken to use the following indices of systemic changes resulting from longlasting occupational exposure to some chemicals: -- ferrum concentration in blood serum, the level of latent capacity to bind ferrum, and the degree of transferrin saturation with ferrum. the examination involved 177 clinically healthy men, aged 18--60. the control group consisted of 40 healthy men, aged 22--60. in 30.5% of persons, deviations were found, which might be induced by disturbances in genera ... | 1979 | 216880 |
| the essence of operating room nursing. the problem of isolation. | | 1979 | 243437 |
| nursing care study: joint replacement. | | 1979 | 243252 |
| any complaints? | | 1979 | 243251 |
| nursing care supplement series b. specialised care. 10. microvascular surgery. | | 1979 | 243250 |
| temporal arteritis and polymyalgia rheumatica. | | 1981 | 243839 |
| myasthenia in patients with dermatomyositis: clinical, electrophysiological and ultrastructural studies. | in 4 patients with clinical signs of dermatomyositis, confirmed by electromyography and muscle biopsy, a form of muscle fatigue was detected which was expressed clinically by predominantly proximal motor deficit, with phonation and deglutition disturbances, slightly influenced by prostigmine. in all patients, stimulation of the ulnar nerve at 3--10 hz induced a decrement of muscle-evoked potentials in abductor digiti minimi and at 15--50 hz an increment at the end of the trains (1.2 sec in durat ... | 1986 | 213537 |
| [breast eczema and breast neoplasms. pseudo paget's disease of the breast]. | | 1987 | 204113 |
| [sebastian kneipp, his life and his work]. | | 1988 | 202779 |
| poly (adp-ribose) polymerase activity in nucleotide permeable cells. | | 1988 | 202502 |
| the chronic patient: perspectives from the 29th institute on hospital & community psychiatry. making rehospitalization part of the plan [proceedings]. | | 1994 | 201559 |
| protein-binding assay for cyclic amp: possible interference by traces of trichloroacetate. | | 1995 | 167609 |
| [the operative surgery of cancer of the tongue]. | | 1998 | 106345 |
| how "fine" is incisional biopsy of melanoma. | | 2012 | 3273029 |
| [ectopic muscles of the back of the hand. short extensor medius muscle]. | | 2013 | 4093324 |
| alkaline reflux gastritis: late results on a controlled trial of diagnosis and treatment. | | 2013 | 3592819 |
| [cardiac autonomic neuropathy in type i diabetics]. | | 2013 | 3660679 |
| localization of sodium pump sites in frog urinary bladder. | [3h]ouabain binding in frog and toad urinary bladder was investigated by short-circuit current (scc), scintillation counting and autoradiographic techniques. scc data and analysis of tissue digests following serosal exposure to ouabain showed that ouabain binding and inhibition of na+ transport was completely reversible in toad bladder whereas, in frog bladder, [3h]ouabain was tightly bound and na+ transport remained suppressed even after a 60-min washout. mucosal exposure of frog bladder to [3h ... | 2002 | 123762 |
| serum tryptophan and cataract. | total serum tryptophan concentrations were measured in 74 cataractous patients and 124 controls. no significant differences were observed in the mean values for the cataractous and normal subjects, 11.4 +/- 2.6 and 11.3 +/- 2.2 micrograms/ml respectively, or in the range of values, 7.4-17.1 and 7.3-17.4 micrograms/ml respectively. these results do not support previous reports that serum tryptophan is elevated in cataractous subjects. | 2013 | 3675914 |
| the family doctor and his 'nudnik (bothersome) patients': an exploratory study. | | 2014 | 4093292 |
| a policy perspective on the united states rehabilitation system. | | 2015 | 5042035 |
| a policy perspective on the united states rehabilitation system. | | 2015 | 5042035 |
| immunity to intracellular bacteria. | | 2016 | 4870271 |
| treatment of the young child with postoperative central diabetes insipidus. | a continuous intravenous infusion of aqueous vasopressin (dosage range, 1.0 to 3.0 mu/kg/h) was administered to two patients (respective ages, 2 weeks and 3 years 1 month) who had postoperative central diabetes insipidus to determine if this mode of therapy is helpful in the very young patient. in both patients the polyuria and serum hyperosmolality were corrected. these findings suggest that an intravenous infusion of aqueous vasopressin can provide satisfactory control of the polyuria and elec ... | 2010 | 2916492 |
| understanding the placenta. part 3: the fetus and the placental barrier. | | 1978 | 242832 |
| structure of carboxymyoglobin in crystals and in solution. | the configuration of the heme-carbonyl group upon binding of carbon monoxide to sperm whale myoglobin (mb) in crystals is evaluated on the basis of infrared spectroscopic methods. multiplets of the totally symmetric c-o stretching mode are observed for the heme-bound ligand near 1933, 1944, and 1967 cm-1, corresponding to three different heme-carbonyl conformers. variations in the relative proportions of these conformers can be induced by incorporation of small fractions of metmb or deoxymb into ... | 1980 | 293700 |
| postoperative irradiation of glioblastomas. results in a randomized series. | a material of 108 patients with glioblastoma is presented. the series was randomized in two groups: cases only operated upon and cases with postoperative irradiation in addition. patients dead within 2 months after operation were excluded in estimating the real value of the postoperative irradiation. the irradiated cases had a 6-month survival rate of 64 per cent and a one-year survival rate of 19 per cent; the non-irradiated cases a 6-month survival rate of 28 per cent and a one-year survival r ... | 1981 | 216238 |
| the nurse who came to play. | | 1981 | 243968 |
| the medicinal mistletoe. | | 1981 | 243851 |
| poliomyelitis in the united states. | | 1981 | 217252 |
| interstitial cystitis: early diagnosis, pathology, and treatment. | in a retrospective review, 52 patients with interstitial cystitis have been studied. patients with persistent lower tract irritative symptoms, repeatedly sterile urine, and negative urine cytology must be suspected of having interstitial cystitis, and a diagnosis of urethral syndrome in such patients is highly questionable until cystoscopy under anesthesia has been performed. we believe that the finding of multiple petechia-like hemorrhages (glomerulations) on the second distention of the bladde ... | 1987 | 213864 |
| [prescribing tiapridal for headache and other painful conditions]. | tiapridal a new molecule of the benzamide family, raises the pain-threshold level in the mesolimbic system. it has been shown to be effective in 66% of cases of headaches and pain resistant to therapy, and 75% of patients with nausea and vomiting associated with headaches. the average dosage is between 150 and 300 mg or day (1/2 to 1 tablet, 3 times a day). the product is extremely well-tolerated. somnolence, the most frequent side-effect was noted in 17% of cases. because of its excellent toler ... | 1988 | 211605 |
| some specific radiological findings and consideration of linitis plastica of the gastrointestinal tract. | linitis plastica denotes a diffuse, intramurally infiltrating, anaplastic carcinoma in a hollow structure resulting in a shrunken organ with thickened walls. microscopically, linitis plastica is characterized by tumor cells in the presence of inflammatory changes with much fibrosis. linitis plastica is found most frequently in the stomach where it may produce the classical "leather-bottle stomach". metastases to the colon are frequent via contiguity along mesenteric fascial planes. therefore, wh ... | 1990 | 185007 |
| giant cell tumor of soft parts. an ultrastructural study. | the light-microscopic and ultrastructural findings in a case of so-called giant cell tumor of soft parts, localized at the dorsal side of the left foot of a 23-years-old male are described. an amputation of the lower extremity was performed and subsequently chemotherapy with adriamycin was given for 3 months. despite the histology and cytologic malignant appearance and the evident vascular invasion, already present at the time of the first excision, the last known status 2 years later seems favo ... | 1990 | 184582 |
| dengue fever. | | 1998 | 19690 |
| [community social service in the field of health education]. | | 2003 | 262313 |
| adaptive changes in the developing brain during intrauterine stress. | maturation of neurological performance in moderately to severely growth-retarded newborn infants (sga) can be accelerated by 3 to 4 weeks or more when compared to the development of appropriately grown infants (aga) of the same gestation. this is particularly the case in multiple pregnancies or pregnancies characterized by maternal hypertension. this clinical finding has been confirmed by neurophysiological studies on the maturation of brainstem auditory evoked responses (baers). the possible me ... | 2006 | 1892222 |
| esorubicin in advanced endometrial cancer: an ineffective and potentially toxic therapy. a southwest oncology group study. | the southwest oncology group conducted a phase ii study of esorubicin treatment in patients with advanced endometrial cancer who had received no prior chemotherapy. twenty of 31 patients were fully evaluable for response and toxicity. there were no clinical responses to treatment and 60% (12/20) of the patients developed severe or life threatening leukopenia on therapy. one evaluable patient was removed from study after a cumulative dose of 150 mg/m2 due to a reduction in left ventricular ejecti ... | 2007 | 1874602 |
| [simplification test in the choice of impression techniques for removable partial dentures]. | | 2008 | 2258231 |
| [sections in harnosand conducted a survey: we stick ourselves, get bitten and get blood on the skin]. | | 2011 | 3176627 |
| black teenage fathers: what happens when the child is born? | this study explored the changes in the lives of teenage fathers, from prenatally to 9 and 18 months postpartum, and compared them to a group of nonfather peers. at 18 months postpartum, more fathers were heads of households and fewer were still in school, although there were no differences in employment status. although only 7.5% of the teenage parents were married, 37% lived together or had daily contact; only three had contact less often than weekly. in the 18 months since the first interview, ... | 2013 | 3725487 |
| [heart diseases in collagenoses]. | | 2014 | 4108772 |
| darwinian fitness and adaptedness in experimental populations of drosophilia willistoni. | | 2015 | 4657883 |
| high-voltage radiography. theory and clinical application. | | 2015 | 4451142 |
| [immunoglobulins normal levels in infancy and childhood (author's transl)]. | | 2015 | 4425181 |
| [detection of quingamine metabolites in biological material]. | | 2015 | 4774704 |
| the use of a residential medical rehabilitation centre. | | 2016 | 5069950 |
| absorption and excretion of parathion by spraymen. | | 2016 | 5031564 |
| x-ray beam uniformity in 250 kv deep therapy units. | | 2016 | 4855430 |
| congenital and surgical anophthalmia. | | 2016 | 4927647 |
| study of rumen metagenome community using qpcr under different diets. | the aim of this study was to detect the major bacteria present in rumen microbiota. here, we performed qpcr based absolute quantitation of selected rumen microbes in rumen fluid of river buffalo adapted to varying proportion of concentrate to roughage diets. animals were adapted to roughage-to-concentrate ratio in the proportion of 100:00 (t1), 75:25 (t2), 50:50 (t3) and 25:75 (t4) respectively for 30 days. at the end of each treatment, rumen fluid was collected at 0 h and 2 h after feeding. it ... | 2014 | 25606402 |
| effects of nitrate addition on rumen fermentation, bacterial biodiversity and abundance. | this study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with ion torrent sequencing and real-time polymerase chain reaction. three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p<0.01), butyrate and isovalerate concentration numerically (p = 0 ... | 2015 | 26194220 |
| nitrate and inhibition of ruminal methanogenesis: microbial ecology, obstacles, and opportunities for lowering methane emissions from ruminant livestock. | ruminal methane production is among the main targets for greenhouse gas (ghg) mitigation for the animal agriculture industry. many compounds have been evaluated for their efficacy to suppress enteric methane production by ruminal microorganisms. of these, nitrate as an alternative hydrogen sink has been among the most promising, but it suffers from variability in efficacy for reasons that are not understood. the accumulation of nitrite, which is poisonous when absorbed into the animal's circulat ... | 2016 | 26904008 |
| synergism of cattle and bison inoculum on ruminal fermentation and select bacterial communities in an artificial rumen (rusitec) fed a barley straw based diet. | this study evaluated the effect of increasing the proportion of bison relative to cattle inoculum on fermentation and microbial populations within an artificial rumen (rusitec). the experiment was a completely randomized design with a factorial treatment structure (proportion cattle:bison inoculum; 0:100, 33:67, 67:33, and 100:0) replicated in two rusitec apparatuses (n = 8 fermenters). the experiment was 15 d with 8 d of adaptation and 7 d of sampling. fermenters were fed a diet of 70:30 barley ... | 2016 | 28018336 |
| two rep genes in small cryptic plasmid pkst21 of escherichia coli. | the complete nucleotide sequence of a small cryptic plasmid pkst21 from escherichia coli was determined. this plasmid is 1,460 bp long with an overall gc content of 51 %. based on sequence analysis, the presence of two segments with different average gc density was observed. the segment with higher gc content revealed 98-90 % similarity to several small plasmids of e. coli and to pcr1 from gram-positive corynebacterium renale. plasmid pkst21 possesses two conversely oriented open reading frames ... | 2013 | 23680975 |
| rapid changes in key ruminal microbial populations during the induction of and recovery from diet-induced milk fat depression in dairy cows. | the ruminant provides a powerful model for understanding the temporal dynamics of gastrointestinal microbial communities. diet-induced milk fat depression (mfd) in the dairy cow is caused by rumen-derived bioactive fatty acids, and is commonly attributed to the changes in the microbial population. the aim of the present study was to determine the changes occurring in nine ruminal bacterial taxa with well-characterised functions, and abundance of total fungi, ciliate protozoa and bacteria during ... | 2015 | 26123320 |
| effect of ethanol and methanol on growth of ruminal bacteria selenomonas ruminantium and butyrivibrio fibrisolvens. | the effect of ethanol and methanol on growth of several ruminal bacterial strains was examined. ethanol concentrations as low as 0.2% had a significant, but moderate, inhibitory effect on lag time or growth over time and 3.3% ethanol significantly inhibited maximum optical density obtained by both selenomonas ruminantium and butyrivibrio fibrisolvens. little growth of either strain occurred at 10% ethanol concentrations. methanol concentrations below 0.5% had little effect on either growth or ma ... | 2015 | 25421629 |
| effects of vanillin, quillaja saponin, and essential oils on in vitro fermentation and protein-degrading microorganisms of the rumen. | this study investigated the effects of vanillin on methanogenesis and rumen fermentation, and the responses of ruminal protein-degrading bacteria to vanillin (at concentrations of 0, 0.76 and 1.52 g/l), essential oils (clove oil, 1 g/l; origanum oil, 0.50 g/l, and peppermint oil, 1 g/l), and quillaja saponin (at concentration of 0 and 6 g/l) in vitro. methane production, degradabilities of feed substrate, and ammonia concentration decreased linearly with increasing doses of vanillin. concentrati ... | 2014 | 23624710 |
| mitigation of methane production from cattle by feeding cashew nut shell liquid. | the effects of cashew nut shell liquid (cnsl) feeding on methane production and rumen fermentation were investigated by repeatedly using 3 holstein nonlactating cows with rumen fistulas. the cows were fed a concentrate and hay diet (6:4 ratio) for 4 wk (control period) followed by the same diet with a cnsl-containing pellet for the next 3 wk (cnsl period). two trials were conducted using cnsl pellets blended with only silica (trial 1) or with several other ingredients (trial 2). each pellet type ... | 2012 | 22916936 |
| characterization of rumen bacterial strains isolated from enrichments of rumen content in the presence of propolis. | propolis presents many biological properties, including antibacterial activities, and has been proposed as an additive in ruminant nutrition. twenty bacterial strains, previously isolated from enrichments of brazilian cow rumen contents in the presence of different propolis extracts (llos), were characterized using phenotyping and 16s rrna identification. seven strains were assigned to streptococcus sp., most likely s. bovis, and were all degrading starch. one amylolytic lactate-utilizing strain ... | 2014 | 25172217 |
| efficient production of mutant phytase (phya-7) derived from selenomonas ruminantium using recombinant escherichia coli in pilot scale. | a mutant gene of rumen phytase (phya-7) was cloned into pet23b(+) vector and expressed in the escherichia coli bl21 under the control of the t7 promoter. the study of fermentation conditions includes the temperature impacts of mutant phytase expression, the effect of carbon supplements over induction stage, the inferences of acetic acid accumulation upon enzyme expression and the comparison of one-stage and two-stage operations in batch mode. the maximum value of phytase activity was reached 107 ... | 2014 | 24686155 |
| effects of the dose and viability of saccharomyces cerevisiae. 1. diversity of ruminal microbes as analyzed by illumina miseq sequencing and quantitative pcr. | this study was conducted to examine effects of the dose and viability of supplemental saccharomyces cerevisiae on the ruminal fermentation and bacteria population and the performance of lactating dairy cows. four ruminally cannulated lactating cows averaging 284±18d in milk were assigned to 4 treatments arranged in a 4×4 latin square design with four 21-d periods. cows were fed a total mixed ration containing 41.7% corn silage, 12.1% brewer's grains, and 46.2% concentrate on a dry matter basis. ... | 2017 | 27837973 |
| effects of corn silage and grass silage in ruminant rations on diurnal changes of microbial populations in the rumen of dairy cows. | here, we examined diurnal changes in the ruminal microbial community and fermentation characteristics of dairy cows fed total mixed rations containing either corn silage (cs) or grass silage (gs) as forage. the rations, which consisted of 52% concentrate and 48% gs or cs, were offered for ad libitum intake over 20 days to three ruminal-fistulated lactating jersey cows during three consecutive feeding periods. feed intake, ruminal ph, concentrations of short chain fatty acids and ammonia in rumen ... | 2016 | 27451293 |
| shifts in rumen fermentation and microbiota are associated with dissolved ruminal hydrogen concentrations in lactating dairy cows fed different types of carbohydrates. | different carbohydrates ingested greatly influence rumen fermentation and microbiota and gaseous methane emissions. dissolved hydrogen concentration is related to rumen fermentation and methane production. | 2016 | 27511925 |
| parameters of fermentation and rumen microbiota of nellore steers fed with different proportions of concentrate in fresh sugarcane containing diets. | the objective of this study was to evaluate the effect of a fresh sugarcane-based diet and different roughage-to-concentrate ratios (70:30, 60:40, 40:60 and 20:80) on the rumen microbiota associated with rumen fermentation parameters and the intake and apparent digestibility of nutrients in nellore steers. eight rumen-cannulated nellore steers (331 ± 8 kg bw) were distributed in a double 4 × 4 latin square design balanced for the control of the residual effect. the ruminal ph decreased (p < 0.01 ... | 2016 | 27415825 |
| changes in rumen microbial community composition during adaption to an in vitro system and the impact of different forages. | this study examined ruminal microbial community composition alterations during initial adaption to and following incubation in a rumen simulation system (rusitec) using grass or corn silage as substrates. samples were collected from fermenter liquids at 0, 2, 4, 12, 24, and 48 h and from feed residues at 0, 24, and 48 h after initiation of incubation (period 1) and on day 13 (period 2). microbial dna was extracted and real-time qpcr was used to quantify differences in the abundance of protozoa, ... | 2016 | 26928330 |
| monitoring of gene expression in fibrobacter succinogenes s85 under the co-culture with non-fibrolytic ruminal bacteria. | fibrobacter succinogenes is one of the most pivotal fibrolytic bacterial species in the rumen. in a previous study, we confirmed enhancement of fiber digestion in a co-culture of f. succinogenes s85 with non-fibrolytic ruminal strains r-25 and/or selenomonas ruminantium s137. in the present study, mrna expression level of selected functional genes in the genome of f. succinogenes s85 was monitored by real-time rt-pcr. growth profile of f. succinogenes s85 was similar in both the monoculture and ... | 2015 | 25354721 |
| interaction of rumen bacteria as assumed by colonization patterns on untreated and alkali-treated rice straw. | colonization patterns of representative rumen bacteria were compared between untreated rice straw (uts) and sodium hydroxide-treated rice straw (shts). uts and shts were incubated in the rumen of sheep for 10 min, 1, 2, 6, 12, 24, 48 and 96 h using the nylon bag method. the population sizes of 13 representative bacterial species or groups were quantified by real-time pcr. the total bacterial population size (abundance) was similar in both uts and shts. fibrobacter succinogenes showed a higher po ... | 2014 | 24612258 |
| use of real-time pcr technique in determination of major fibrolytic and non fibrolytic bacteria present in indian surti buffaloes (bubalus bubalis). | in the milk industry in india, buffalo breeds are most commonly used for milk production. efficiency of fiber digestion in ruminants is critical for animal productivity. bacteria play an important role in fiber digestion and utilization. absolute quantification real-time pcr was used to quantify ten bacterial species in rumen fluid of surti buffalo fed green fodder, dry roughage and compound concentrate mixture. abundance of each target taxon was calculated as a fraction of the total 16s rrna ge ... | 2013 | 24053023 |
| effect of non-starch-polysaccharide-degrading enzymes as feed additive on the rumen bacterial population in non-lactating cows quantified by real-time pcr. | the effects of non-starch-polysaccharide-degrading enzymes, added to a maize silage- and grass silage-based total mixed ration (tmr) at least 14 h before feeding, on the rumen bacterial population were investigated. six non-lactating holstein friesian cows were allocated to three treatment groups using a duplicate 3 × 3 latin square design with three 31-day periods (29 days of adaptation and 2 days of sampling). treatments were control tmr [69% forage and 31% concentrates on a dry matter (dm) ba ... | 2013 | 23216628 |
| involvement of recently cultured group u2 bacterium in ruminal fiber digestion revealed by coculture with fibrobacter succinogenes s85. | in a previous study, we reported the ecological significance of uncultured bacterial group u2 in the rumen. in this study, the involvement of a recently cultured group u2 bacterium, strain r-25, in fiber digestion was tested in coculture with the fibrolytic bacterium fibrobacter succinogenes s85. dry matter (dm) digestion, growth and metabolites were examined in culture using rice straw as the carbon source. although strain r-25 did not digest rice straw in monoculture, coculture of strain r-25 ... | 2012 | 22849722 |
| effect of disodium fumarate on microbial abundance, ruminal fermentation and methane emission in goats under different forage: concentrate ratios. | this study investigated the effects of disodium fumarate (df) on methane emission, ruminal fermentation and microbial abundance in goats under different forage (f) : concentrate (c) ratios and fed according to maintenance requirements. four ruminally fistulated, castrated male goats were used in a 4 × 4 latin square design with a 2 × 2 factorial arrangement of treatments and the main factors being the f : c ratios (41 : 59 or 58 : 42) and df supplementation (0 or 10 g/day). df reduced methane pr ... | 2012 | 22717128 |
| characterization of rumen bacterial diversity and fermentation parameters in concentrate fed cattle with and without forage. | to determine the effects of the removal of forage in high-concentrate diets on rumen fermentation conditions and rumen bacterial populations using culture-independent methods. | 2012 | 22533638 |
| a fibrolytic enzyme additive for lactating holstein cow diets: ruminal fermentation, rumen microbial populations, and enteric methane emissions. | the objective was to determine if supplementing a dairy cow diet with an exogenous fibrolytic enzyme additive (econase rde; ab vista, marlborough, wiltshire, uk) altered fermentation, ph, and microbial populations in the rumen or enteric methane (ch(4)) emissions. in a companion study, this enzyme additive improved efficiency of fat-corrected milk production in a dose-dependent manner by up to 11% for early lactation dairy cows. nine ruminally cannulated, lactating holstein cows were used in a r ... | 2012 | 22365224 |
| effects of an extract of plant flavonoids (bioflavex) on rumen fermentation and performance in heifers fed high-concentrate diets. | to study the effects of an extract of plant flavonoids [bioflavex (fl)] in cattle fed high-concentrate diets, 2 experiments were designed. in the first experiment, the effects of bioflavex on the development of rumen acidosis was evaluated in 8 holstein-friesian crossbreed heifers (451 kg; sem 14.3 kg of bw) using a crossover design. each experimental period lasted 22 d; from d 1 to 20, the animals were fed rye grass, on d 21 the animals were fasted, and on d 22, rumen acidosis was induced by ap ... | 2012 | 22829622 |
| bacterial community diversity associated with different levels of dietary nutrition in the rumen of sheep. | the sheep rumen microbial community plays an important role in animal performance and the environment. few studies have paid close attention to the impact of different levels of dietary nutrition on rumen microbial populations. a total of 112 healthy tan sheep were selected and randomly allotted to one of four dietary treatments (groups i, ii, iii, and iv). each treatment included four replicated pens with seven sheep each for a total of 28 sheep per treatment. the sheep were fed four diets with ... | 2017 | 28175950 |
| comparative evaluation of rumen metagenome community using qpcr and mg-rast. | microbial profiling of metagenome communities have been studied extensively using mg-rast and other related metagenome annotation databases. although, database based taxonomic profiling provides snapshots of the metagenome architecture, their reliability needs to be validated through more accurate methods. here, we performed qpcr based absolute quantitation of selected rumen microbes in the liquid and solid fraction of the rumen fluid of river buffalo adapted to varying proportion of concentrate ... | 2013 | 24025701 |
| effects of extracts of humulus lupulus (hops) and yucca schidigera applied alone or in combination with monensin on rumen fermentation and microbial populations in vitro. | β-acids in hops (humulus lupulus) and saponins in yucca (yucca schidigera) have been found to possess antimicrobial properties similar to that of monensin and could be an alternative to in-feed antibiotics. the effects of monensin (mon) and ethanol extracts of hops (he) and y. schidigera (ye) alone and in combination with mon were assessed on ruminal microbial composition and fermentation in vitro of a barley-based diet. | 2013 | 23483574 |
| development of a species-specific pcr assay for identification of the strictly anaerobic bacterium selenomonas lacticifex found in biofilm-covered surfaces in brewery bottling halls. | in recent years, beer-spoilage cases from strictly anaerobic bacteria have risen in frequency, in connection with the production of non-pasteurized, non-alcohol and low-alcoholic beers and with the lowering of dissolved oxygen in the packaged beer. selenomonas lacticifex, found in brewer's yeast and in biofilms covering some surfaces in brewery bottling area, is considered to be a beer-spoilage organism. this study aims to develop s. lacticifex-specific pcr assay. the objective of this study was ... | 2014 | 25066481 |
| effect of saccharomyces cerevisiae on alfalfa nutrient degradation characteristics and rumen microbial populations of steers fed diets with different concentrate-to-forage ratios. | live yeast (saccharomyces cerevisiae) constitutes an effective additive for animal production; its probiotic effect may be related to the concentrate-to-forage ratio (ctfr). the objective of this study was to assess the effects of s. cerevisiae (sc) on fiber degradation and rumen microbial populations in steers fed diets with different levels of dietary concentrate. ten simmental × local crossbred steers (450 ± 50 kg bw) were assigned to a control group or an sc group. both groups were fed the s ... | 2014 | 24883184 |
| lysine decarboxylase with an enhanced affinity for pyridoxal 5-phosphate by disulfide bond-mediated spatial reconstitution. | lysine decarboxylase (ldc) catalyzes the decarboxylation of l-lysine to produce cadaverine, an important industrial platform chemical for bio-based polyamides. however, due to high flexibility at the pyridoxal 5-phosphate (plp) binding site, use of the enzyme for cadaverine production requires continuous supplement of large amounts of plp. in order to develop an ldc enzyme from selenomonas ruminantium (srldc) with an enhanced affinity for plp, we introduced an internal disulfide bond between ala ... | 2017 | 28095457 |
| crystal structure and pyridoxal 5-phosphate binding property of lysine decarboxylase from selenomonas ruminantium. | lysine decarboxylase (ldc) is a crucial enzyme for acid stress resistance and is also utilized for the biosynthesis of cadaverine, a promising building block for bio-based polyamides. we determined the crystal structure of ldc from selenomonas ruminantium (srldc). srldc functions as a dimer and each monomer consists of two distinct domains; a plp-binding barrel domain and a sheet domain. we also determined the structure of srldc in complex with plp and cadaverine and elucidated the binding mode ... | 2016 | 27861532 |
| engineering disulfide bonds in selenomonas ruminantium β-xylosidase by experimental and computational methods. | homotetrameric β-xylosidase from selenomonas ruminantium (sxa) is one of the most efficient enzymes known for the hydrolysis of cell wall hemicellulose. sxa shows a rapid rate of activity loss at temperatures above 50°c. in this study, we have introduced two inter-subunit disulfide bridges with one point mutation. lys237 was chosen to be replaced with cysteine since it interacts with the same residue in the opposite subunit. while ph optimum, temperature profile and catalytic efficiency of the m ... | 2017 | 27818293 |
| peptidoglycan-associated outer membrane protein mep45 of rumen anaerobe selenomonas ruminantium forms a non-specific diffusion pore via its c-terminal transmembrane domain. | the major outer membrane protein mep45 of selenomonas ruminantium, an anaerobic gram-negative bacterium, comprises two distinct domains: the n-terminal s-layer homologous (slh) domain that protrudes into the periplasm and binds to peptidoglycan, and the remaining c-terminal transmembrane domain, whose function has been unknown. here, we solubilized and purified mep45 and characterized its function using proteoliposomes reconstituted with mep45. we found that mep45 forms a nonspecific diffusion c ... | 2016 | 27310312 |
| cloning and high-level expression of β-xylosidase from selenomonas ruminantium in pichia pastoris by optimizing of ph, methanol concentration and temperature conditions. | β-xylosidase and several other glycoside hydrolase family members, including xylanase, cooperate together to degrade hemicelluloses, a commonly found xylan polymer of plant-cell wall. β-d-xylosidase/α-l-arabinofuranosidase from the ruminal anaerobic bacterium selenomonas ruminantium (sxa) has potential utility in industrial processes such as production of fuel ethanol and other bioproducts. the optimized synthetic sxa gene was overexpressed in methylotrophic pichia pastoris under the control of ... | 2016 | 27154901 |
| divergent utilization patterns of grass fructan, inulin, and other nonfiber carbohydrates by ruminal microbes. | fructans are an important nonfiber carbohydrate in cool season grasses. their fermentation by ruminal microbes is not well described, though such information is needed to understand their nutritional value to ruminants. our objective was to compare kinetics and product formation of orchardgrass fructan (phlein; phl) to other nonfiber carbohydrates when fermented in vitro with mixed or pure culture ruminal microbes. studies were carried out as randomized complete block designs. all rates given ar ... | 2016 | 26601577 |
| complete genome sequence of selenomonas ruminantium subsp. lactilytica will accelerate further understanding of the nature of the class negativicutes. | selenomonas ruminantium subsp. lactilytica, a strictly anaerobic ruminal bacterium, possesses typical gram-negative cell surface structure comprising cytoplasmic membrane, peptidoglycan layer and outer membrane, whereas its 16s rrna-based taxonomy shows that the bacteria belongs to gram-positive firmicutes. complete genome analysis showed that genes or gene clusters involved in gram-negative cell structure were scattered in the s. ruminantium genome, and might provide the new insight of phylogen ... | 2015 | 25837814 |
| effect of soybean meal and soluble starch on biogenic amine production and microbial diversity using in vitro rumen fermentation. | this study was conducted to investigate the effect of soybean meal (sm) and soluble starch (ss) on biogenic amine production and microbial diversity using in vitro ruminal fermentation. treatments comprised of incubation of 2 g of mixture (expressed as 10 parts) containing different ratios of sm to ss as: 0:0, 10:0, 7:3, 5:5, 3:7, or 0:10. in vitro ruminal fermentation parameters were determined at 0, 12, 24, and 48 h of incubation while the biogenic amine and microbial diversity were determined ... | 2015 | 25557675 |
| effects of nitrate addition to a diet on fermentation and microbial populations in the rumen of goats, with special reference to selenomonas ruminantium having the ability to reduce nitrate and nitrite. | this study investigated the effects of dietary nitrate addition on ruminal fermentation characteristics and microbial populations in goats. the involvement of selenomonas ruminantium in nitrate and nitrite reduction in the rumen was also examined. as the result of nitrate feeding, the total concentration of ruminal volatile fatty acids decreased, whereas the acetate : propionate ratio and the concentrations of ammonia and lactate increased. populations of methanogens, protozoa and fungi, as esti ... | 2015 | 25439583 |
| influence of fermented tannery solid waste on morphological, biochemical, yield and nutritional responses of tomato plants. | the non-tanned proteinaceous tannery solid waste animal fleshing (anfl), containing high nutritive value, was hydrolyzed using bacteria selenomonas ruminantium hm000123 through submerged (smf) and solid-state (ssf) fermentation processes. in addition, the effects of anfl fermentative hydrolysate on growth, yield and biochemical properties of tomato plants were investigated. the treatments included t1 (smf-anfl), t2 (ssf-anfl), t3 (recommended dose of npk fertilizers) and a control without any am ... | 2015 | 25296938 |
| an efficient biosurfactant-producing bacterium selenomonas ruminantium ct2, isolated from mangrove sediment in south of thailand. | biosurfactant-producing bacteria, isolate ct2, was isolated from mangrove sediment in the south of thailand. the sequence of the 16s rrna gene from isolate ct2 showed 100 % similarity with selenomonas ruminantium. the highest biosurfactant production (5.02 g/l) was obtained when the cells were grown on minimal salt medium containing 15 g/l molasses and 1 g/l commercial monosodium glutamate supplemented with 1 g/l nacl, 0.1 g/l leucine, 5 % (v/v) inoculum size at 30 °c and 150 rpm after 54 h of c ... | 2013 | 22932808 |
| variability of putative rep gene cassettes in selenomonas ruminantium plasmids. | characteristic feature of the most of selenomonas ruminantium cryptic plasmids is the presence of short, conserved sequences encompassing the gene for replication protein creating a potential rep gene cassette. pcr-based experiment was designed to analyse the genetic organization of putative plasmid rep modules and to assess s. ruminantium plasmid biodiversity. analysed pcr amplicons contained single open reading frames encoding for putative replication proteins. while most of the derived protei ... | 2012 | 22900647 |
| molecular basis for the maintenance of envelope integrity in selenomonas ruminantium: cadaverine biosynthesis and covalent modification into the peptidoglycan play a major role. | polyamine is a small organic polycation composed of a hydrocarbon backbone with multiple amino groups which ubiquitously exists in all living organisms from bacteria to higher animals. the critical step of polyamine biosynthesis generally includes the amino acid-decarboxylating reaction to produce the primary diamines, such as a synthesis of putrescine (nh(3)(+)·(ch(2))(4)·nh(3)(+)) from ornithine, and cadaverine (nh(3)(+)·(ch(2))(5)·nh(3)(+)) from lysine, which are catalyzed by pyridoxal-5'-pho ... | 2012 | 22878384 |
| effect of sunflower and marine oils on ruminal microbiota, in vitro fermentation and digesta fatty acid profile. | this study using the rumen simulation technique (rusitec) investigated the changes in the ruminal microbiota and anaerobic fermentation in response to the addition of different lipid supplements to a ruminant diet. a basal diet with no oil added was the control, and the treatment diets were supplemented with sunflower oil (2%) only, or sunflower oil (2%) in combination with fish oil (1%) or algae oil (1%). four fermentation units were used per treatment. rusitec fermenters were inoculated with r ... | 2017 | 28676798 |
| effects of saccharomyces cerevisiae fermentation products on performance and rumen fermentation and microbiota in dairy cows fed a diet containing low quality forage. | a possible option to meet the increased demand of forage for dairy industry is to use the agricultural by-products, such as corn stover. however, nutritional value of crop residues is low and we have been seeking technologies to improve the value. a feeding trial was performed to evaluate the effects of four levels of saccharomyces cerevisiae fermentation product (scfp; original xp; diamond v) on lactation performance and rumen fermentation in mid-lactation holstein dairy cows fed a diet contain ... | 2017 | 28465826 |
| effect of ginkgo extract supplementation on in vitro rumen fermentation and bacterial profiles under different dietary conditions. | ginkgo extract was applied to a batch culture study and evaluated for its potential as a feed additive for ruminant animals under different forage-to-concentrate (f:c) ratios (1:9, 3:7, 5:5, 7:3 and 9:1). rumen fluid was mixed with respective diet and incubated at 39°c for 24 h with and without ginkgo extract (1.6% fruit equivalent in culture). methane production was significantly decreased by ginkgo extract, with the greatest reductions found in the 5:5 (41.9%) followed by the 7:3 ratios (36.7% ... | 2017 | 28707415 |