Publications

isolation, enumeration, and characteristics of proteolytic ruminal bacteria.	fulghum, robert s. (virginia polytechnic institute, blacksburg) and w. e. c. moore. isolation, enumeration, and characteristics of proteolytic ruminal bacteria. j. bacteriol. 85:808-815. 1963.-colony counts of proteolytic ruminal bacteria in the order of 10(9) organisms per g of whole rumen contents, and total colony counts in the order of 2 to 3 x 10(9) organisms per g, were obtained from rumen contents of cattle fed a maintenance ration of hay and grain. the proteolytic counts averaged 38% of ...	1963	14044947
growth factors for selenomonas ruminantium.		1963	13954887
[cytochromes].		1965	4287863
enzymatic studies of pure cultures of rumen microorganisms.	joyner, a. e., jr. (university of california, davis), and r. l. baldwin. enzymatic studies of pure cultures of rumen microorganisms. j. bacteriol. 92:1321-1330. 1966.-the activities of enzymes representing the major pathways of carbohydrate metabolism and anaerobic electron transport in cell-free extracts of whole rumen contents have been reported. the effects of diet upon the activities of several enzymes suggested that enzymatic measurements might prove useful for the study of rumen metabolism ...	1966	4380801
the effect of acid mucopolysaccharides on the activation energy of collagen.		1967	4227781
function of growth factors for rumen microorganisms. i. nutritional characteristics of selenomonas ruminantium.	nutritional characteristics of selenomonas ruminantium var. lactilytica isolated from a sheep rumen were studied. the organism required for growth the addition of a clarified rumen fluid to a trypticase-yeast extract medium with either lactate or glucose as an energy source. the requirement for rumen fluid was found to be satisfied by volatile fatty acids in glucose media and by biotin in lactate media. straight-chain saturated fatty acids with c(3) to c(10) carbon skeleton had been found to be ...	1967	6020417
function of growth factors for rumen microorganisms. ii. metabolic fate of incorporated fatty acids in selenomonas ruminantium.		1968	5689520
metabolic interlock. regulatory interactions exerted between biochemical pathways.		1969	4306282
preparation of immune rabbit sera with neutralizing activity against human cytomegalovirus and varicella-zoster virus.		1970	4316778
mechanism of propionate formation by selenomonas ruminantium, a rumen micro-organism.		1970	5530770
carbon dioxide requirement of various species of rumen bacteria.	the carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. increasing concentrations of co(2) were added as nahco(3) to a specially prepared co(2)-free medium which was tubed and inoculated under nitrogen. prior depletion of co(2) in the inoculum was found to affect the level of requirement; however, the complexity and buffering capacity of the medium did not appear to be involved. an absolute requirement for co(2) was observed for e ...	1971	5541030
isolation, culture, and fermentation characteristics of selenomonas ruminantium var. bryantivar. n. from the rumen of sheep.	large forms of selenomonas sp. were isolated from the sheep rumen on a rumen fluid-glucose-agar medium by using a differential centrifugation technique to purify the inoculum. the cells from the six isolated strains were curved, gram-negative, strictly anaerobic crescents, and rapidly motile by flagella attached to the concave side of the cell. one or more of the volatile fatty acids were essential for growth. none of the strains produced indole or reduced nitrate. all strains grew on fructose, ...	1971	4323298
[clinical methods for the detection of estrus in sows].		1971	5006043
[the replicative complexes of sendai virus].		1971	4332921
atpase activity and palmitate oxidation of hepatic mitochondria in energy production in endotoxemia.		1971	4256350
value of electrical pacing and beta adrenergic blockers in the prevention of fatal ventricular arrhythmias.		1971	4399445
chemical structure of lipid a of selenomonas ruminantium.		1971	5562340
induced structural transitions in trna.		1971	248323
acid-base balance and sodium-to-chloride ratio in diets of laying hens.		1972	5007114
ftc-insulin conjugates. i. preparation of fluorescein thiocarbamyl-insulin conjugates.		1973	4579445
dynamic stiffness of papillary muscle during contraction and relaxation.		1973	4691288
dynamic stiffness of papillary muscle during contraction and relaxation.		1973	4691288
boar acrosin. ii. classification, inhibition, and specificity studies of a proteinase from sperm acrosomes.		1973	4752950
ultrastructural studies on selenomonas ruminantium from the sheep rumen.		1973	4773919
a simplified digital method for predicting anesthetic uptake and distribution.		1973	4777734
analysis of bypass activation of c3 by endotoxic lps and loss of this potency.		1973	4122408
propionate formation from cellulose and soluble sugars by combined cultures of bacteroides succinogenes and selenomonas ruminantium.	succinate is formed as an intermediate but not as a normal end product of the bovine rumen fermentation. however, numerous rumen bacteria are present, e.g., bacteroides succinogenes, which produce succinate as a major product of carbohydrate fermentation. selenomonas ruminantium, another rumen species, produces propionate via the succinate or randomizing pathway. these two organisms were co-cultured to determine if s. ruminantium could decarboxylate succinate produced by b. succinogenes. when en ...	1973	4796955
the construction of lambda transducing phages containing deletions defining regulatory elements of the lac and trp operons in e. coli.		1974	4601264
[inhibitory effect of caffeine on the absorption of ethanol in the rat].		1974	4463934
[left cervicotomy for esophageal foreign body].		1974	4283188
the presence and function of cytochromes in selenomonas ruminantium, anaerovibrio lipolytica and veillonella alcalescens.		1974	4362619
non-thrombocytopenic purpura associated with cat-scratch disease.		1974	4812014
isolation of ureolytic peptostreptococcus productus from feces using defined medium; failure of common urease tests.	colony counts of fecal samples from three persons, obtained by using a chemically defined anaerobic roll-tube medium (containing glucose, maltose, glycerol, minerals, hemin, b-vitamins, methionine, volatile fatty acids, sulfide, bicarbonate, agar, carbon dioxide (gas phase), and 1 mm nh(4) (+) as main nitrogen source), averaged 60% of the 8.8 x 10(10) bacteria per g obtained when 0.2% trypticase and 0.05% yeast extract were added to the otherwise identical medium. when 0.2% vitamin-free casitone ...	1974	4213672
conditional lethal mutants of vesicular stomatitis virus.		1975	169102
on the significance of the increase in homovanillic acid (hva) caused by antipsychotic drugs in corpus striatum and limbic forebrain.	the effect of various antipsychotic drugs on the blockade of dopaminergic receptors in striatum and limbic forebrain was examined by establishing dose-response curves for the increase in hva and for the antagonism of d-amphetamine-induced rotation in rats with unilateral lesions of the substantia nigra. a good quantitative correlation was found between dopaminergic blockade in the striatum as reflected by the ed100 for striatal hva increase and the ed50 for rotational antagonism and the occurren ...	1975	242028
h2 production by selenomonas ruminantium in the absence and presence of methanogenic bacteria.	selenomonas ruminantium is a nonsporeforming anaerobe that ferments carbohydrates primarily to lactate, propionate, acetate and co2. h2 production by this species has not been previously reported. we found, however, that some strains produce trace amounts of h2 which can be detected by sensitive gas chromatographic procedures. h2 production is increased markedly, in some cases almost 100-fold, when the selenomonads are co-cultured with methane-producing bacteria. growth of the methane-producing ...	1975	804850
relationship of lactate dehydrogenase specificity and growth rate to lactate metabolism by selenomonas ruminantium.	a lactate-fermenting strain of selenomonas ruminantium (hd4) and a lactatenonfermenting strain (ga192) were examined with respect to the stereoisomers of lactate formed during glucose fermentation, the stereoisomers of lactate fermented by hd4, and the characteristics of the lactate dehydrogenases of the strains. ga192 formed l-lactate and hd4 formed l-lactate and small amounts of d-lactate from glucose. hd4 fermended l- but not d-lactate. both strains contain nicotinamide adenine dinucleotide ( ...	1975	174490
reversible hypothyroidism in growth hormone-deficient children treated with human growth hormone.	six children with human growth hormone (hgh) deficiency became hypothyroid during the course of their therapy with hgh. this was accompanied by a decreasing growth rate, clinical symptoms of hypothyroidism and decreased serum t4 concentrations. three of the 6 patients returned to the euthyroid state, both clinically and biochemically, with cessation of hgh therapy, and reinstitution of hgh precipitated hypothyroidism again in 2 of the three. the patients who remained hypothyroid have evidence of ...	1975	165211
a structural study of pig liver glyceraldehyde-3-phosphate dehydrogenase.	a substantial portion of the primary structure of pig liver glyceraldehyde-3-phosphate dehydrogenase has been investigated and the results compared with those previously reported for the pig muscle enzyme. liver and muscle glyceraldehyde-3-phosphate dehydrogenases show the same amino acid content, and the first n-terminal residues occur in the same sequence. no differences in n-terminal residues and amino acid composition have been evidenced by analysis of several tryptic peptides, which account ...	1976	182238
human plasma lipoproteins as accelerators of prothrombin activation.	the activation rate of bovine prothrombin by factor xa and ca2+ has long been known to be greatly enhanced by addition of phospholipid. upon substitution of human plasma lipoproteins for phospholipid (cephalin) in this activation system, only very low density lipoprotein enhances prothrombin activation. low density lipoprotein and high density lipoprotein have no stimulatory effect on prothrombin activation. on the other hand, the sonicated lipid extracts from very low, low, and high density lip ...	1976	182693
the fine structure of a tri-lamellar body in various species of anabaena.		1976	184493
particulate carbonic anhydrase in homogenates of human kidney.	about 2% of human kidney carbonic anhydrase (carbonate hydro-lyase, ec 4.2.1.1) has been found in particulate fractions. its distribution in the particulate fractions obtained by differential centrifugation suggests that it may be concentrated in the brush border. the particulate enzyme is like red cell carbonic anhydrace c in its susceptibility to inhibition by anions. particulate carbonic anhydrase is firmly bound to the membrane and is not released by incubation at ph 10.6 and 37 degrees c or ...	1976	184830
sv40 and adenovirus early functions involved in dna replication and transformation.		1976	184845
cross-reactions between bordetella pertussis and twenty-eight other bacterial species.	cross-reactions between b. pertussis and 28 other bacterial species were studied by various quantitative immunoelectrophoretic methods. a sonicated b. pertussis antigen preparation and a corresponding pooled rabbit antiserum were used as reference system. two of the b. pertussis antigens were cross-reactive with antigens from 17, respectively 19, other bacterial species mainly gram-negative species. as judged by absorption of antibodies, the degree of cross-reactivity of these b. pertussis antig ...	1976	187010
lactic acid-utilizing bacteria in ruminal fluid of a steer adapted from hay feeding to a high-grain ration.	a mature, rumen-cannulated steer fed coastal bermuda grass hay for 8 weeks was adapted to a high-grain ration by stepwise increases in grain over a period of 4 weeks. the grain rations had concentrate-to-roughage ratios of 40:60, 70:30, and 85:15 and were fed for 10, 7, and 11 days, respectively. numerical estimates of lactate-utilizing bacteria in ruminal fluid of the steer the last 3 days each ration was fed were made by colony counts. lactate-utilizing bacteria were identified as megasphaera ...	1976	1275348
influence of ch4 production by methanobacterium ruminantium on the fermentation of glucose and lactate by selenomonas ruminantium.	a method is described for increasing the production of h2 from glucose or lactate by selenomonas ruminantium by sequential transfers in media containing pregrown methanobacterium ruminantium. the methanogen uses the h2 formed by the selenomonad to reduce co2 to ch4. analysis of fermentation products from glucose showed that lactate was the major product formed from glucose by s. ruminantium alone. several sequential transfers in the presence of the methanogen caused a marked decrease in lactate ...	1977	596874
effect of ruminal lactic acid-utilizing bacteria on adaptation of cattle to high-energy rations.	heifers, unadapted to a concentrate ration, were intraruminally inoculated (1 dose) with cultures of ruminal lactic acid-utilizing bacteria or with ruminal fluid from a steer adapted to a concentrate ration. inoculation with cultures (1 l) of selenomonas ruminantium or megasphaera elsdenii did not produce better average daily weight gains or feed efficiency of heifers fed a high-energy ration for 21 days, if these values were compared with the performance of noninoculated heifers. average daily ...	1977	883707
role of dna repair in natural resistance of rat ascites hepatomas to nitrogen mustard.	mechanism of natural resistance of rat ascites hepatomas to nitrogen mustard was examined with tumors, especially ah-13 and ah-44, sensitive and resistant to the chemical, respectively. there was found little difference in the uptake and binding of the chemical among the two lines. in contrast, dna repair activity of the resistant line was higher than that of the sensitive line. this result suggested that natural resistance of ah-44 to the drug may be, at least partially, due to its higher activ ...	1977	193752
[acute diarrhea in childhood--new aspects].		1977	202052
fibrous histiocytoma of the tarsus.	a 21-year-old white man had an asymptomatic eyelid mass that had slowly enlarged for one year. on clinical examination the lesion was well circumscribed; it appeared subconjunctivally but provoked no conjunctival inflammation, and had a consistency suggestive of cartilage. microscopic examination showed it was matted and composed of elongated fibro-histiocytes and an evenly distributed population of xanthoma cells and touton giant cells. the lesion was easily removed by local excision because of ...	1977	202162
neuromuscular transmission in arterioles of guinea-pig submucosa.	1. intracellular recordings were made from arterioles lying in the submucosa of guinea-pig small intestine. 2. low frequency perivascular nerve stimulation evoked subthreshold excitatory junction potentials which facilitated. 3. higher frequency stimulation caused summation of excitatory junction potentials and the initiation of muscle action potentials. 4. arteriolar constriction was only observed following the initiation of a muscle action potential.	1977	202698
intestinal calcium binding protein levels in the one month old turkey poult.	intestinal calcium binding protein cabp) was studied in one month old poults which had been fed a vitamin d3-deficient diet unsupplemented or with 399, 600, 900 or 1200 i.u. vitamin d3 added per kg. diet. cabp levels increased with vitamin d3 supplementation up to 600 i.u. but showed no further increase with higher dietary vitamin d3. poults supplemented with 600 to 1200 i.u. vitamin d3/kg. diet had an average of 1.03 mg. cabp/g. of duodenal mucosa cells collected. the calcium binding activity o ...	1977	203920
[diaphyseal bone tuberculosis].	the authors report a case of diaphyseal bony tuberculosis, in which the diagnosis was not made for a long time as bony tuberculosis is considered exceptional and owing to the presence of a staphylococcus which oriented the diagnosis towards osteomyelitis. bony tuberculosis certainly affects more easily immigrant patients, in whom one may see multifocal forms, but also affects those who, by their environment, might imagine they are protected, and it is not exceptional to see unifocal forms.	1977	204049
alkaline phosphatase activity of rumen bacteria.	of the 54 strains of rumen bacteria examined for alkaline phosphatase (apase) production, 9 of 33 gram-negative strains and none of 21 gram-positive strains produced the enzyme. the apase of the cells of the three strains of bacteroides ruminicola that produced significant amounts of the enzyme was located in the periplasmic area of the cell envelope, whereas the enzyme was located in the strains of selenomonas ruminantium and succinivibrio dextrinosolvens was associated with the outer membrane. ...	1977	563216
[human science. behavioral science. 4. social awareness and human behavior--a theme in sociological behavior theory concerning the patient].		1977	242705
thyrotrophin releasing hormone-induced growth hormone and prolactin release: physiological studies in intact rats and in hypophysectomized rats bearing an ectopic pituitary gland.	to determine how the sensitivity of the ectopic anterior pituitary gland to the gh-releasing effect of thyrotrophin releasing hormone (trh) might be affected by the time lapse from transplantation, trh (0-15 and 0-6 microng) was injected i.v. into hypophysectomized (hypox)-transplanted rats under urethane anaesthesia 1,3,8,15,30 and 60 days after transplantation, and plasma samples were taken 5 and 10 min later. baseline gh values gradually decreased with time from about 16-0 ng/ml (1 day) to ab ...	1977	404376
demonstration of 5'-methylthioadenosine phosphorylase activity in various rat tissues. some properties of the enzyme from rat lung.	an enzyme (5'-methylthioadenosine phosphorylase) that catalyzes the phosphorolytic cleavage of 5'-methylthioadenosine to 5-methylthioadenosine to 5-methylthioribose-1-phosphate and adenine was found in various rat tissues. liver and lung had the highest enzyme activities and heart the lowest, most of the activity (greater than 90%) was recovered in soluble tissue fractions. the enzyme from rat lung was purified about 30-fold by ph treatment (nh4)2so4 fractionation, and gel filtration. the enzyme ...	1978	415762
effects of heavy metals and other trace elements on the fermentative activity of the rumen microflora and growth of functionally important rumen bacteria.	the inhibitory effects of high concentrations of essential and non-essential trace elements were tested on the rumen microflora using the rate of fermentation in vitro as the assay. the elements (and the concentration causing 50% inhibition) in decreasing order of toxicity were hg2+ (20 microgram/ml), cu2+ (21 microgram/ml), cr6+ (70 microgram/ml), se4+ (73 microgram/ml), ni2+ (160 microgram/ml), cd2+ (175 microgram/ml), as3+ (304 microgram/ml) and as5+ (1610 microgram/ml). the elements tested t ...	1978	565671
production of branched-chain volatile fatty acids by certain anaerobic bacteria.	net production of isobutyric acid, isovaleric acid, and 2-methylbutyric acid by cultures of bacteroides ruminicola and megasphaera elsdenii on media that contained trypticase or casein hydrolysate continued (up to 5 days) after growth had ceased. only trace quantities of these acids were produced in a medium that contained a mixture of amino acids that did not include the branched-chain amino acids. m. elsdenii produced increased quantities of the branched-chain fatty acids in a medium that cont ...	1978	566082
polysomes and polysomal mrnas in sv40-infected cv-1 cells. in vivo observations.	sv40-specific polysomes are relatively larger than host polysomes. both 16 s and 19 s virus-specific late mrnas are found associated with these polysomes and they are present in a ratio of approximately 2 : 1. the rates at which these virus-specific mrnas are translated are the same, so that the relative amounts of virus-specific polypeptides synthesized in the virus-infected cells is determined by the relative amounts of 16 s and 19 s mrnas coding for them.	1978	208616
[mononucleosis syndrome and thrombocytopenic purpura in q fever (rickettsia burneti)].	case of a patient with a mononucleosis syndrome and thrombocytopenic purpura during q fever. this etiology could lead to a systematic search for a rickettsial cause for such a symptomatology with a negative paul-bunnell-davidson reaction.	1978	211604
mammary tumors, hepatocellular carcinomas, and pancreatic islet changes in c3h-avy mice.	studies of tumor incidence and assorted lesions found in 187 c3h-avy mice throughout their natural life-spans revealed the following: hepatocellular carcinomas occurred in 54.3% of males, mammary carcinomas in 95% of females, pancreatic islet cell adenomas in 9.4% of males and in no females, and pancreatic islet cell hyperplasia in 41% of males and 23% of fefemales. islet cell hyperplasia and adenomas appeared to consist predominantly of alpha and delta cells. multiple tumors, or hyperplasia, or ...	1978	203711
an unstable donor-recipient dna complex in transformation of bacillus subtilis.	in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ...	1978	106232
control of lactate production by selenomonas ruminantium: homotropic activation of lactate dehydrogenase by pyruvate.	selenomonas ruminantium produced one mole of d(-)-lactate per mole of glucose used at all dilution rates in ammonia-limited continuous culture. in contrast, lactate production varied according to the dilution rate when glucose was the limiting nutrient. at dilution rates of less than 0.2 h-1, acetate and propionate were the main fermentation products and lactate production was low. at dilution rates above 0.2 h-1, the pattern changed to one of high lactate production similar to that under ammoni ...	1978	103995
nutritional requirements of selenomonas ruminantium for growth on lactate, glycerol, or glucose.	the nutritional requirements of selenomonas ruminantium hd4 for growth on glucose, glycerol, or lactate were investigated to clarify the results of previous studies and to relate the nutrition of the organism to its physiology. the organism required l-aspartate, co(2), p-aminobenzoic acid, and biotin for growth on a lactate-salts medium that contained small amounts of dithiothreitol. aspartate could be replaced by l-malate or fumarate but not by succinate or l-asparagine. requirements for growth ...	1978	16345271
understanding the placenta. part 3: the fetus and the placental barrier.		1978	242832
[concentration of serum iron and transferrin saturation with iron as indices of changes resulting from chronic exposure to chemicals in industry].	an attempt was undertaken to use the following indices of systemic changes resulting from longlasting occupational exposure to some chemicals: -- ferrum concentration in blood serum, the level of latent capacity to bind ferrum, and the degree of transferrin saturation with ferrum. the examination involved 177 clinically healthy men, aged 18--60. the control group consisted of 40 healthy men, aged 22--60. in 30.5% of persons, deviations were found, which might be induced by disturbances in genera ...	1979	216880
the essence of operating room nursing. the problem of isolation.		1979	243437
nursing care study: joint replacement.		1979	243252
any complaints?		1979	243251
nursing care supplement series b. specialised care. 10. microvascular surgery.		1979	243250
comparison of substrate affinities among several rumen bacteria: a possible determinant of rumen bacterial competition.	five rumen bacteria, selenomonas ruminantium, bacteroides ruminicola, megasphaera elsdenii, streptococcus bovis, and butyrivibrio fibrisolvens were grown in continuous culture. estimates of substrate affinities were derived from lineweaver-burk plots of dilution rate versus substrate concentration. each bacterium was grown on at least four of the six substrates: glucose, maltose, sucrose, cellobiose, xylose, and lactate. wide variations in substrate affinities were seen among the substrates util ...	1979	16345358
comparison of maintenance energy expenditures and growth yields among several rumen bacteria grown on continuous culture.	maintenance energy expenditures were mesured for five rumen bacteria, selenomonas ruminantium, butyrivibrio fibrisolvens, bacteroides ruminicola, megasphaera elsdenii, and streptococcus bovis, by using a complex medium with glucose as the carbon source. large differences (as high as 8.5-fold) in maintenance energy expenditures were seen among these bacteria. the suggestion is made that maintenance requirements could be a significant determinant of bacterial competition in the rumen. theoretical ...	1979	16345359
effects of combinations of substrates on maximum growth rates of several rumen bacteria.	five rumen bacteria, selenomonas ruminantium, bacteroides ruminicola, megasphaera elsdenii, butyrivibrio fibrisolvens, and streptococcus bovis were grown in media containing nonlimiting concentrations of glucose, sucrose, maltose, cellobiose, xylose and/or lactate. each bacterium was grown with every substrate that it could ferment in every possible two-way combination. only once did a combination of substrates result in a higher maximum growth rate than that observed with either substrate alone ...	1979	16345360
effect of monensin and lasalocid-sodium on the growth of methanogenic and rumen saccharolytic bacteria.	it is thought that monensin increases the efficiency of feed utilization by cattle by altering the rumen fermentation. we studied the effect of monensin and the related ionophore antibiotic lasalocid-sodium (hoffman-laroche) on the growth of methanogenic and rumen saccharolytic bacteria in a complex medium containing rumen fluid. ruminococcus albus, ruminococcus flavefaciens, and butyrivibrio fibrisolvens were inhibited by 2.5 mug of monensin or lasalocid per ml. growth of bacteroides succinogen ...	1979	16345418
purification of antibodies to influenza a virus structural proteins by affinity chromatography, and their participation in haemagglutination-inhibition, neutralization and enzyme-linked immunosorbent assay.	affinity chromatography was used to purify rabbit antibodies to common and strain-specific antigeneic determinants of haemagglutinin, to neuraminidase, and to a combination of the internal proteins of influenza a viruses. the purity of the antibodies was assessed by haemagglutination-inhibition, enzyme-linked immunosorbent assay (elisa) and competition elisa. the antibodies were examined for their participation in neutralization and haemagglutination-inhibition assays, and in elisa. elisa was fo ...	1979	93960
partial amino acid sequences of rabbit and rat beta 2-microglobulins.		1979	93696
an investigation of the optimal conditions for the in vivo production of immunologically sensitised rat mast cells.	experiments were designed to develop an optimal method for inducing in vivo production of sensitised peritoneal mast cells. rats of different strains were sensitised with whole egg-white and killed at suitable intervals to harvest the peritoneal mast cells. release of histamine was induced in vitro by both whole egg-white and its major protein constituents, and assayed by a standard spectrofluorometric method. wistar rats showed higher levels of sensitisation than black-hooded lister rats; it wa ...	1979	92507
spontaneous interictal spiking in the awake kindled rat.	the spontaneous interictal spike (sis) noted in eeg recordings is a signal of the abnormally excitable behavior of neurons in an epileptic focus. this study provides a detailed, quantitative, temporal and spatial profile of sis in the amygdaloid kindled rat. the reproducible nature of the development and decline of sis in kindling provides a useful model for biochemical study of the mechanisms that initiate and regulate sis.	1979	91486
cytotoxicity of oxidation derivatives of cholesterol on cultured aortic smooth muscle cells and their effect on cholesterol biosynthesis.	aortic smooth muscle cell death is an important initial lesion of atherosclerosis. a number of autooxidation products of cholesterol which has been recognized recently has the capability of inducing rabbits' aortic smooth cell death in vitro. twelve oxidation derivatives of cholesterol, available commercially, were dissolved in small amounts of ethanol, then added to the culture medium at levels not exceeding 0.8%. the medium contained 10% fetal calf's serum which served as an in situ vehicle fo ...	1979	34998
the development of mesosomes of staphylococcus aureus during autolysis.	mesosomes of staphylococcus aureus were examined morphologically under the electron microscope. three different methods of specimen preparation (thin section, freeze-fracture, and negative staining) were used to eliminate artifacts due to sample processing. mesosomes were rarely seen in intact cells but were quite distinct in autolysed cells incubated in 1.2m sucrose -0.33m acetate buffer (ph 5.8) at 25 degrees c. the phospholipid content increased by 20% of the control as autolysis proceeded. s ...	1979	91308
[effect of human antigens on the biological properties of the causative agents of typhoid fever and paratyphoid b].		1979	91281
pathobiology of cortical neurons in metabolic and unclassified amentias.	visualization of the neuron in its entirety through the use of the rapid golgi method has permitted detection of several pathobiological features of neurons that are intimately associated with profound mental retardation in infants and children. in cases of unclassified mental retardation, dendrites and particularly dendritic spines exhibit severe developmental abnormalities. dendritic spines, the postsynaptic components of axospinodendritic synapses, may be absent or abnormally long and thin in ...	1979	217050
the potential histogenic relationship of the peripheral nerve to synovioma.	the belief that synoviomas differentiate from the synovial membrane has been widely accepted. absolute proof is lacking, mainly because precancerous synovial atypia has not been documented in human tissue. during the last 50 years, pain has emerged as one of the significant features of synovioma, occurring very early in the course of the disease in some patients. gross relationship to nerve, cumulative histological data, and theoretical relationships with three other cancers of neural origin sug ...	1979	225019
condensation--inhibition by 33258-hoechst of centromeric heterochromatin in prematurely condensed mouse chromosomes.		1979	91529
[infection as indicator of finding complex environmental noxious agents within a short time (author's transl)].	we do not know whether complexes of chemical agents produce an accumulation of their respective noxious effects or whether these effects weaken each other and we have no exact methods to quantify these effects. knowledge of such methods would be important for investigation of environmental health. in the course of researches how to influence infectious diseases by altering natural resistance we have found that it might be possible to use an indicator method: animals exposed to such a light envir ...	1979	92117
office microbiology.		1979	111268
use of micro-elisa for quantitating antibody to trypanosoma cruzi and trypanosoma rangeli.	of 229 residents of a panamanian village where both trypanosoma cruzi and trypanosoma rangeli are endemic, 52% had antibody to one or both species by the enzyme-linked immunosorbent assay (elisa), 26% were positive by complement fixation, 17% were positive by direct agglutination, and 32% were positive on the basis of clinical impression. although the sensitivity of elisa makes it the procedure of choice for sero-epidemiologic studies, there does appear to be some serologic cross-reactivity betw ...	1979	116559
surface properties and in vitro functional capacities of human t lymphocyte subsets separated by linear density gradients.	human lymphocytes derived from various central and peripheral sources were separated on linear density gradients (ldg). cells from individual density fractions were tested in parallel for: the capacity to form nonimmune rosettes with neuraminidase-treated srbc, the number of surface-associated htla, and in vitro proliferative responses to mitogenic lectins and alloantigens. heterogeneous density distribution profiles were obtained for all sources of human t cells and revealed an organ specificit ...	1979	156234
changes in lung ultrastructure following heterologous and homologous serum albumin infusion in the treatment of hemorrhagic shock.	the object of this study was to compare the ultrastructure pulmonary effects of the infusion of homologous and heterologous serum albumin solution in the treatment of hemorrhagic shock in baboons. adult baboons subjected to hemorrhagic shock were resuscitated with either baboon serum albumin, human serum albumin, or ringer's lactate solution. the lungs were fixed in vivo with potassium pyroantimony, a solution which produces electron dense interstitial precipitation of sodium. the lungs from ani ...	1979	106780
some effects of uncouplers and inhibitors on growth and electron transport in rumen bacteria.	uncouplers and inhibitors of electron transport affected growth and electron transport of rumen bacteria in various ways. selenomonas ruminantium was not affected by inhibitor and uncoupler concentrations which affected growth and electron transport of bacteroides ruminicola, b. succinogenes, and butyrivibrio fibrisolvens. inhibitors, when active, led to accumulation of reduced electron carriers before the site of action, but differences were found among organisms in the site of action of these ...	1979	457609
synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in veillonella, selenomonas, and bacteriodes species.	evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. cultures were grown in media containing unlabeled energy substrates plus [14c]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. our results indicate alpha-ketoglutarate synthase functions in selenomo ...	1979	533772
transformation of mercuric chloride and methylmercury by the rumen microflora.	the microflora in strained rumen fluid did not methylate or volatilize 203hg2+ at detectable rates. however, there was an exponential decay in the concentration of added ch3hg+, which was attributed to demethylation. the major product of demethylation was metallic mercury (hg0), and it was released as a volatile product from the reaction mixture. demethylation occurred under both anaerobic and aerobic conditions. the rate of demethylation was proportional to the concentration of added ch3hg+-hg ...	1979	539820
suppression of phytohemagglutinin response by fungi from a "leukemia" house.	fungal isolates from the house of a husband and wife who both developed acute myelomonocytic leukemia were assayed for effects on the in vivo response to phytohemagglutinin in guinea pigs. skin responses to intradermal phytohemagglutinin were measured following injections of sterile fungal extracts. isolates of penicillium canescens, curvularia, fusarium sambucinum, fusarium equiseti and trichoderma koningii from the leukemia-associated house depressed the responses to phytohemagglutinin, but no ...	1979	291385
inactive mrna-protein complexes from mouse sarcoma-180 ascites cells.	mouse sarcoma ascites cells do not utilize fully their capacity for protein synthesis. a considerable portion of their ribosomes occur as inactive monomers. also, a substantial amount of the cellular mrna is in the form of ribonucleoprotein particles that sediment in the 20-70s range. this is indicated both by measurements of poly(a) content and by translation of the rna in cell-free systems. the population of polypeptides synthesized under the direction of the rna from these particles is less h ...	1979	293664
a possible folding pathway of bovine pancreatic rnase.	a theoretical pathway for the folding of rnase into its native conformation is derived from the contact map computed from crystallographic coordinates. the pathway is based on the hypothesis of tanaka and scheraga, according to which localized conformations stabilized by short- and medium-range interactions form before those conformational features that are stabilized primarily by long-range interactions. the pathway deduced from the contact map agrees with experimental information on intermedia ...	1979	293701
biochemical and topographical studies on escherichia coli cell surface. iv. giant spheroplast formation from a filamentous cell.	long, nonseptate filamentous cells consisting of 5 to 40 single-cell unit lengths were formed from escherichia coli surface mutant ont-3 by treatment with a sublethal concentration of sodium dodecyl sylfate. as distinct from several other elongated cells (e.g., thymine-starved filaments), it was found here that stable giant spheroplasts, 5 to 10 micrometers in diameter, were produced by the action of lysozyme in the presence of bovine serum albumin via the gradual fusion of distinct spheroplasti ...	1979	374397
isolation and characterization of outer and inner membranes of selenomonas ruminantium: lipid compositions.	the isolation procedure and characterization of the outer and inner membranes from selenomonas ruminatium cells, a strictly anaerobic bacterium, are described. the metabolic fate of [14c]decanoate incorporated into the outer and inner membranes was examined. the percent distribution of radioactivities in the outer and inner membranes was about 40 and 50% of the total incorporated activity, respectively. approximately 47% of the radioactivity incorporated into the outer membrane was recovered in ...	1980	7364719
outer membrane proteins and cell surface structure of selenomonas ruminantium.	the protein compositions of the membrane preparations from selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfate- and two-dimensional (first, isoelectric focusing; second, sodium dodecyl sulfate) polyacrylamide slab gel electrophoresis. the outer membrane from both glucose- and lactate-grown cells contained two major proteins with apparent molecular weights of 42,000 and 40,000. these proteins existed as peptidoglycan-associated proteins in the oute ...	1980	7364720
effect of ph on the efficiency of growth by pure cultures of rumen bacteria in continuous culture.	a total of 10 strains of rumen bacteria, selenomonas ruminantium hd4, megasphaera elsdenii b159, butyrivibrio fibrisolvens a38, streptococcus bovis jb1, lactobacillus vitulinus ga1, bacteroides ruminicola b14, b. ruminicola ga33, ruminococcus albus 7, ruminococcus flavefaciens c94, and bacteroides succinogenes s85, were grown in energy-limiteh of the medium reservoir was lowered approximately 0.3 ph units, and the energy source concentration remaining in the culture vessel, optical density, cell ...	1980	7387158