Publications

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infantile cirrhosis with hereditary alpha 1 -antitrypsin deficiency. clinical improvement in two siblings. 20144196606
[electrophoretic study of blood proteins in patients with sequelae of cerebrovascular disorders]. 20144187852
[cephalometric analysis of the effect of the chin sling]. 20154534519
[radiographic cassette with goniometer and its use on orthopedic tables in operations of femoral neck fractures]. 20154705124
[radiographic cassette with goniometer and its use on orthopedic tables in operations of femoral neck fractures]. 20154705124
four keys to 100 per cent success in tetanus prophylaxis. 20154613194
an accurate system for evaluating gamma ray collimators. 20154438442
response of body weight to a low carbohydrate, high fat diet in normal and obese subjects. 20154703054
[occupational medicine problems of an aviation physician]. 20154701759
[occupational medicine problems of an aviation physician]. 20154701759
an enzymatic determination of ammonia in biological fluids. 20154284306
low false-alarm rates in signal detection by marine mammals. 20154833081
[structure-activity relationship in a series of homologous cis and trans 2-alkyl-1,3-dioxolanes isomers]. 20154426382
membrane lipid pattern and non-electrolytes permeability in salmo trutta l. red blood cells. 20154353913
[criterion for optimization of sampling size]. 20154683655
[clinical course and nursing care of patients with decubitus ulcer--use of pillows stuffed with buckwheat chaff]. 20154482661
sexual maturation in juvenile hypothyroidism. 20154357385
rosette-forming cells and mif-producing cells in penicillin allergies. 20154542234
structure and properties of hemocyanins. xiv. reassociation of helix pomatia alpha-hemocyanin. 20154453008
wolbachia pipientis: a potential candidate for combating and eradicating dengue epidemics in pakistan.dengue virus syndrome is an emerging global health challenge which is endemic in tropical countries like pakistan. in recent years dengue incidences have increased considerably in different areas of pakistan with more sever impacts on urban and peri-urban populations. this review is an effort to highlight the changing epidemiology of dengue fever, role of government of pakistan in disease management and control using preventive and community based approaches in the region. moreover, there is an ...201526706669
mosaic composition of riba and wspb genes flanking the virb8-d4 operon in the wolbachia supergroup b-strain, wstr.the obligate intracellular bacterium, wolbachia pipientis (rickettsiales), is a widespread, vertically transmitted endosymbiont of filarial nematodes and arthropods. in insects, wolbachia modifies reproduction, and in mosquitoes, infection interferes with replication of arboviruses, bacteria and plasmodia. development of wolbachia as a tool to control pest insects will be facilitated by an understanding of molecular events that underlie genetic exchange between wolbachia strains. here, we used n ...201526400107
supergroup c wolbachia, mutualist symbionts of filarial nematodes, have a distinct genome structure.wolbachia pipientis is possibly the most widespread endosymbiont of arthropods and nematodes. while all wolbachia strains have historically been defined as a single species, 16 monophyletic clusters of diversity (called supergroups) have been described. different supergroups have distinct host ranges and symbiotic relationships, ranging from mutualism to reproductive manipulation. in filarial nematodes, which include parasites responsible for major diseases of humans (such as onchocerca volvulus ...201526631376
a rare human case of dirofilaria repens infection in the subcutaneous posterior thorax with molecular identification.the emergence of dirofilarial infections in asia including vietnam is a clinically significant threat to the community. we here report a rare case of subcutaneous dirofilaria repens infection on the posterior thoracic wall in a young woman presenting a painful, itchy, and palpable nodule. the adult worm was identified by mitochondrial cox1 and nuclear its-2 sequence determination. the diagnosis was additionally confirmed by 16s rrna sequencing of the endosymbiont wolbachia pipientis commonly co- ...201526174827
[drosophila melanogaster cell culture as an experimental model to study recombination in wolbachia pipientis].wolbachiapipientis is an obligate intracellular endosymbiont that commonly infects arthropods. comparative genomic studies of wolbachia reveal traces of numerous events of intergenic and intragenic recombination. the molecular mechanisms of recombination in wolbachia are not currently known. we conducted experimental verification of the possibility of recombination of two strains of wolbachia: wmel and wri, after using these strains for double infection of the dm2008wb1 (d. melanogaster) cell cu ...201527055293
wolbachia utilize host actin for efficient maternal transmission in drosophila melanogaster.wolbachia pipientis is a ubiquitous, maternally transmitted bacterium that infects the germline of insect hosts. estimates are that wolbachia infect nearly 40% of insect species on the planet, making it the most prevalent infection on earth. the bacterium, infamous for the reproductive phenotypes it induces in arthropod hosts, has risen to recent prominence due to its use in vector control. wolbachia infection prevents the colonization of vectors by rna viruses, including drosophila c virus and ...201525906062
effects of mimosine on wolbachia in mosquito cells: cell cycle suppression reduces bacterial abundance.the plant allelochemical l-mimosine (β-[n-(3-hydroxy-4-pyridone)]-α-aminopropionic acid; leucenol) resembles the nonessential amino acid, tyrosine. because the obligate intracellular alphaproteobacterium, wolbachia pipientis, metabolizes amino acids derived from host cells, the effects of mimosine on infected and uninfected mosquito cells were investigated. the ec50 for mimosine was 6-7 μm with aedes albopictus c7-10 and c/wstr cell lines, and was not influenced by infection status. mosquito cel ...201526019119
dynamics of wolbachia pipientis gene expression across the drosophila melanogaster life cycle.symbiotic interactions between microbes and their multicellular hosts have manifold biological consequences. to better understand how bacteria maintain symbiotic associations with animal hosts, we analyzed genome-wide gene expression for the endosymbiotic α-proteobacteria wolbachia pipientis across the entire life cycle of drosophila melanogaster. we found that the majority of wolbachia genes are expressed stably across the d. melanogaster life cycle, but that 7.8% of wolbachia genes exhibit rob ...201526497146
strain-specific and pooled genome sequences for populations of drosophila melanogaster from three continents.to contribute to our general understanding of the evolutionary forces that shape variation in genome sequences in nature, we have sequenced genomes from 50 isofemale lines and six pooled samples from populations of drosophila melanogaster on three continents. analysis of raw and reference-mapped reads indicates the quality of these genomic sequence data is very high. comparison of the predicted and experimentally-determined wolbachia infection status of these samples suggests that strain or samp ...201525717372
passage of wolbachia pipientis through mutant drosophila melanogaster induces phenotypic and genomic changes.wolbachia pipientis is a nearly ubiquitous, maternally transmitted bacterium that infects the germ line of insect hosts. estimates are that wolbachia infects 40 to 60% of insect species on the planet, making it one of the most prevalent infections on earth. however, we know surprisingly little about the molecular mechanisms used by wolbachia to infect its hosts. we passaged wolbachia through normally restrictive drosophila melanogaster hosts, bottlenecking wolbachia through stochastic segregatio ...201525452279
species in wolbachia? proposal for the designation of 'candidatus wolbachia bourtzisii', 'candidatus wolbachia onchocercicola', 'candidatus wolbachia blaxteri', 'candidatus wolbachia brugii', 'candidatus wolbachia taylori', 'candidatus wolbachia collembolicola' and 'candidatus wolbachia multihospitum' for the different species within wolbachia supergroups.wolbachia are highly extended bacterial endosymbionts that infect arthropods and filarial nematodes and produce contrasting phenotypes on their hosts. wolbachia taxonomy has been understudied. currently, wolbachia strains are classified into phylogenetic supergroups. here we applied phylogenomic analyses to study wolbachia evolutionary relationships and examined metrics derived from their genome sequences such as average nucleotide identity (ani), in silico dna-dna hybridization (ddh), g+c conte ...201526189661
[genotypic diversity of wolbachia pipientis in native and invasive harmonia axyridis pall., 1773 (coleoptera, coccinellidae) populations].the distribution and variability of reproductive symbiotic wolbachia pipientis bacteria were studied in seven native and six invasive h. axyridis populations. wolbachia-infected individuals were found in two invasive and two native populations. we demonstrated for the first time an infection of invasive h. axyridis populations with wolbachia. two new molecular forms of wolbachia were detected by a system of multilocus typing. the supergroup a wolbachia was found for the first time in h. axyridis ...201526601484
the drosophila bag of marbles gene interacts genetically with wolbachia and shows female-specific effects of divergence.many reproductive proteins from diverse taxa evolve rapidly and adaptively. these proteins are typically involved in late stages of reproduction such as sperm development and fertilization, and are more often functional in males than females. surprisingly, many germline stem cell (gsc) regulatory genes, which are essential for the earliest stages of reproduction, also evolve adaptively in drosophila. one example is the bag of marbles (bam) gene, which is required for gsc differentiation and germ ...201526291077
population genomics of infectious and integrated wolbachia pipientis genomes in drosophila ananassae.coevolution between drosophila and its endosymbiont wolbachia pipientis has many intriguing aspects. for example, drosophila ananassae hosts two forms of w. pipientis genomes: one being the infectious bacterial genome and the other integrated into the host nuclear genome. here, we characterize the infectious and integrated genomes of w. pipientis infecting d. ananassae (wana), by genome sequencing 15 strains of d. ananassae that have either the infectious or integrated wana genomes. results indi ...201526254486
wolbachia infection in a natural parasitoid wasp population.the maternally transmitted bacterium wolbachia pipientis is well known for spreading and persisting in insect populations through manipulation of the fitness of its host. here, we identify three new wolbachia pipientis strains, whho, whho2 and whho3, infecting hyposoter horticola, a specialist wasp parasitoid of the glanville fritillary butterfly. the whho strain (st435) infects about 50% of the individuals in the åland islands in finland, with a different infection rate in the two mitochondrial ...201526244782
[drug effects on peripheral circulation in postthrombotic syndrome]. 20154678612
[methodology in nutrition surveys among preschool children]. 20154666043
the wolbachia wo bacteriophage proteome in the aedes albopictus c/wstr1 cell line: evidence for lytic activity?wolbachia pipientis (rickettsiales), an obligate intracellular alphaproteobacterium in insects, manipulates host reproduction to maximize invasion of uninfected insect populations. modification of host population structure has potential applications for control of pest species, particularly if wolbachia can be maintained, manipulated, and genetically engineered in vitro. although wolbachia maintains an obligate mutualism with genome stability in nematodes, arthropods can be co-infected with dist ...201526427709
the concept of general experience: age and strategies in guessing future events. 20154683201
comparative analysis of wolbachia genomes reveals streamlining and divergence of minimalist two-component systems.two-component regulatory systems are commonly used by bacteria to coordinate intracellular responses with environmental cues. these systems are composed of functional protein pairs consisting of a sensor histidine kinase and cognate response regulator. in contrast to the well-studied caulobacter crescentus system, which carries dozens of these pairs, the streamlined bacterial endosymbiont wolbachia pipientis encodes only two pairs: ccka/ctra and plec/pled. here, we used bioinformatic tools to co ...201525809075
french invasive asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to vietnamese autochthonous relatives.the asian tiger mosquito aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. originating from asia, it has invaded a wide range of eco-climatic regions worldwide. the insect-associated microbiota is now recognized to play a significant role in host biology. while genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. to address this su ...201526441903
[serum glycoproteins in peritonitis]. 20154703286
u wave alternans and increased ventricular irritability. 20154749392
the impact of wolbachia on virus infection in mosquitoes.mosquito-borne viruses such as dengue, west nile and chikungunya viruses cause significant morbidity and mortality in human populations. since current methods are not sufficient to control disease occurrence, novel methods to control transmission of arboviruses would be beneficial. recent studies have shown that virus infection and transmission in insects can be impeded by co-infection with the bacterium wolbachia pipientis. wolbachia is a maternally inherited endosymbiont that is commonly found ...201526556361
[long-term follow-up of reconstruction of the pulmonary artery using homograft]. 20154676526
wolbachia reduces the transmission potential of dengue-infected aedes aegypti.dengue viruses (denv) are the causative agents of dengue, the world's most prevalent arthropod-borne disease with around 40% of the world's population at risk of infection annually. wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits replication of the virus in the mosquito. the wolbachia strain wmel, which has been introduced into the mosquito vector, aedes aegypti, has been shown to invade and spread to near fix ...201526115104
discovery of putative small non-coding rnas from the obligate intracellular bacterium wolbachia pipientis.wolbachia pipientis is an endosymbiotic bacterium that induces a wide range of effects in its insect hosts, including manipulation of reproduction and protection against pathogens. little is known of the molecular mechanisms underlying the insect-wolbachia interaction, though it is likely to be mediated via the secretion of proteins or other factors. there is an increasing amount of evidence that bacteria regulate many cellular processes, including secretion of virulence factors, using small non ...201525739023
[use and abuse of psychopharmacological agents. generalities on clinical effects and indications]. 20154393424
application of wmelpop wolbachia strain to crash local populations of aedes aegypti.the endosymbiotic bacteria wolbachia pipientis (wmel strain) has been successfully established in several populations of aedes aegypti, the primary dengue vector. the virulent wolbachia strain wmelpop is known to cause several pathological impacts (increased egg mortality, life shortening, etc.) reducing overall fitness in the mosquito ae. aegypti. increased egg mortality could substantially reduce egg banks in areas with a lengthy monsoonal dry season, and be employed to eliminate local populat ...201526204449
one-appointment technic to form a crown base. 20154619603
bursicon: its effect on tyrosine permeation into insect haemocytes. 20154640774
[evaluation of early and long term results of the surgical treatment of dupuytren's contracture in controversy problem--partial or total fascietomy]. 20154664976
proceedings: adolescent psychiatry and the concepts of "open system" in the service of ego growth and evolvement. 20154522942
a new translucent cement for dentistry. the glass ionomer cement. 20154501690
[ergonomics is not only for the practice and laboratory]. 20154510683
[pathology of cholera--especially on the autopsy cases in the phillipines (author's transl)]. 20154472807
blepharochalasis. 20154824166
analog computer simulation of log(k)-ph profiles. 20154982391
[rapid identification of amanitins in mushroom tissues (author's transl)]. 20154408098
[quantum doloris: evaluation from 0 to 10]. 20154408005
determination of lead using charged particle activation analysis. 20154814232
[50th anniversary of the sanitary service of the ussr]. 20154585046
susceptibility of mammalian (hamster) cell culture to infection with type ii leukosis virus (jm). 20154575488
inhibition of end-plate desensitization by sodium. 20154546362
bleeding gastritis induced by long-term release aspirin. 20154546274
a new single-drop technique for the observation of dynamic blood coagulation. 20154558871
[learning from history]. 20154526672
[differential diagnosis of eosinophilic granuloma and deep marginal periodontitis]. 20154512704
studies on muscle rigidity: droperidol, diazepam, and promethazine. 20165062116
theory of competitive binding of iron by different transferrins. 20164824920
an analysis of meningitis cases admitted to strathclyde hospital, motherwell 1949-71. 20164794861
risk associated with the release of wolbachia-infected aedes aegypti mosquitoes into the environment in an effort to control dengue.in an effort to eliminate dengue, a successful technology was developed with the stable introduction of the obligate intracellular bacteria wolbachia pipientis into the mosquito aedes aegypti to reduce its ability to transmit dengue fever due to life shortening and inhibition of viral replication effects. an analysis of risk was required before considering release of the modified mosquito into the environment.201627047911
the influence of larval competition on brazilian wolbachia-infected aedes aegypti mosquitoes.with field releases starting in brazil, particular interest must be given to understanding how the endosymbiotic bacterium wolbachia pipientis affects aedes aegypti mosquitoes with a brazilian genetic background. currently, there is limited information on how the bacterium affects phenotypic traits such as larval development rate, metabolic reserves and morphometric parameters in ae. aegypti. here, we analyze for the first time, the effect of wolbachia on these key phenotypes and consider how th ...201627183820
wolbachia-based dengue virus inhibition is not tissue-specific in aedes aegypti.dengue fever, caused by the dengue virus (denv), is now the most common arbovirus transmitted disease globally. one novel approach to control denv is to use the endosymbiotic bacterium, wolbachia pipientis, to limit denv replication inside the primary mosquito vector, aedes aegypti. wolbachia that is naturally present in a range of insects reduces the capacity for viruses, bacteria, parasites and fungi to replicate inside insects. wolbachia's mode of action is not well understood but may involve ...201627855218
establishment of a wolbachia superinfection in aedes aegypti mosquitoes as a potential approach for future resistance management.wolbachia pipientis is an endosymbiotic bacterium estimated to chronically infect between 40-75% of all arthropod species. aedes aegypti, the principle mosquito vector of dengue virus (denv), is not a natural host of wolbachia. the transinfection of wolbachia strains such as walbb, wmel and wmelpop-cla into ae. aegypti has been shown to significantly reduce the vector competence of this mosquito for a range of human pathogens in the laboratory. this has led to wmel-transinfected ae. aegypti curr ...201626891349
rapid and non-destructive detection and identification of two strains of wolbachia in aedes aegypti by near-infrared spectroscopy.the release of wolbachia infected mosquitoes is likely to form a key component of disease control strategies in the near future. we investigated the potential of using near-infrared spectroscopy (nirs) to simultaneously detect and identify two strains of wolbachia pipientis (wmelpop and wmel) in male and female laboratory-reared aedes aegypti mosquitoes. our aim is to find faster, cheaper alternatives for monitoring those releases than the molecular diagnostic techniques that are currently in us ...201627362709
inappropriate secretion of antidiuretic hormone in infectious pulmonary disease. 20165074478
[results after 5 years of trial therapy in cutaneous tuberous angiomas]. 20165035075
radical prostatectomy in the management of carcinoma of the prostate: probable causes of some therapeutic failures. 20165033963
aging under glass. 20164929456
[on a proposed law making obligatory the declaration of viral hepatitis and concerning disinfection]. 20164928868
occlusion and prosthetic dentistry. 20164892616
[primary and secondary plastic surgery after mandibular resection. (measures for restoration of normal facial structure and jaw function)]. 20164869337
molecular detection of wolbachia pipientis in natural populations of mosquito vectors of dirofilaria immitis from continental portugal: first detection in culex theileri.wolbachia pipientis (rickettsiales: rickettsiaceae) protects mosquitoes from infections with arboviruses and parasites. however, the effect of its co-infection on vector competence for dirofilaria immitis (spirurida: onchocercidae) in the wild has not been investigated. this study aimed to screen vectors of d. immitis for wpip, to characterize these, and to investigate a possible association between the occurrence of w. pipientis and that of the nematode. the presence of w. pipientis was assesse ...201627279553
actin in the alga, chara corallina. 20164835552
[primary trigeminal neuralgia--odontologic points of view]. 20164766396
editorial: investigative dermatology. 20164758728
photochemistry of diazonium salts. i. synthesis of 4-fluoroimidazoles, 4-fluorohistamine, and 4-fluorohistidine. 20164730663
downregulation of aedes aegypti chromodomain helicase dna binding protein 7/kismet by wolbachia and its effect on dengue virus replication.dengue virus (denv) is a mosquito-transmitted virus imposing a significant burden on human health around the world. since current control strategies are not sufficient, there is an urgent need to find alternative methods to control denv transmission. it has been demonstrated that introduction of wolbachia pipientis in aedes aegypti mosquitoes can impede denv transmission with the mechanism(s) not fully understood. recently, a number of studies have found the involvement of chromodomain dna bindi ...201627827425
wolbachia blocks currently circulating zika virus isolates in brazilian aedes aegypti mosquitoes.the recent association of zika virus with cases of microcephaly has sparked a global health crisis and highlighted the need for mechanisms to combat the zika vector, aedes aegypti mosquitoes. wolbachia pipientis, a bacterial endosymbiont of insect, has recently garnered attention as a mechanism for arbovirus control. here we report that aedes aegypti harboring wolbachia are highly resistant to infection with two currently circulating zika virus isolates from the recent brazilian epidemic. wolbac ...201627156023
near-infrared spectroscopy, a rapid method for predicting the age of male and female wild-type and wolbachia infected aedes aegypti.estimating the age distribution of mosquito populations is crucial for assessing their capacity to transmit disease and for evaluating the efficacy of available vector control programs. this study reports on the capacity of the near-infrared spectroscopy (nirs) technique to rapidly predict the ages of the principal dengue and zika vector, aedes aegypti. the age of wild-type males and females, and males and females infected with wmel and wmelpop strains of wolbachia pipientis were characterized u ...201627768689
genomes of candidatus wolbachia bourtzisii wdaca and candidatus wolbachia pipientis wdacb from the cochineal insect dactylopius coccus (hemiptera: dactylopiidae).dactylopius species, known as cochineal insects, are the source of the carminic acid dye used worldwide. the presence of two wolbachia strains in dactylopius coccus from mexico was revealed by pcr amplification of wsp and sequencing of 16s rrna genes. a metagenome analysis recovered the genome sequences of candidatus wolbachia bourtzisii wdaca (supergroup a) and candidatus wolbachia pipientis wdacb (supergroup b). genome read coverage, as well as 16s rrna clone sequencing, revealed that wdacb wa ...201627543297
comparative genomics of two closely related wolbachia with different reproductive effects on hosts.wolbachia pipientis are obligate intracellular bacteria commonly found in many arthropods. they can induce various reproductive alterations in hosts, including cytoplasmic incompatibility, male-killing, feminization, and parthenogenetic development, and can provide host protection against some viruses and other pathogens. wolbachia differ from many other primary endosymbionts in arthropods because they undergo frequent horizontal transmission between hosts and are well known for an abundance of ...201627189996
wolbachia pipientis should not be split into multiple species: a response to ramírez-puebla et al., "species in wolbachia? proposal for the designation of 'candidatus wolbachia bourtzisii', 'candidatus wolbachia onchocercicola', 'candidatus wolbachia blaxteri', 'candidatus wolbachia brugii', 'candidatus wolbachia taylori', 'candidatus wolbachia collembolicola' and 'candidatus wolbachia multihospitum' for the different species within wolbachia supergroups". 201627021523
[how to fight parasitic infectious diseases with bacteria. the case of wolbachia pipientis].in nature, no individual can live in isolation; hence, living organisms are forced to interact with each other. this necessity has led many organisms to establish heterogeneous relations to enhance their ability to adapt to the environment, thus acquiring evolutionary advantages. these relationships are sometimes so intense, that on the long term the organisms may lose their individual identity. an example of these associations is the endosymbiotic ones, where eukaryote organisms generally harbo ...201625354059
chimeric coupling proteins mediate transfer of heterologous type iv effectors through the escherichia coli pkm101-encoded conjugation machine.bacterial type iv secretion systems (t4sss) are composed of two major subfamilies, conjugation machines dedicated to dna transfer and effector translocators for protein transfer. we show here that the escherichia coli pkm101-encoded conjugation system, coupled with chimeric substrate receptors, can be repurposed for transfer of heterologous effector proteins. the chimeric receptors were composed of the n-terminal transmembrane domain of pkm101-encoded traj fused to soluble domains of vird4 homol ...201627432829
a response to lindsey et al. "wolbachia pipientis should not be split into multiple species: a response to ramírez-puebla et al.". 201627033416
the effect of temperature on wolbachia-mediated dengue virus blocking in aedes aegypti.dengue fever, caused by dengue virus (denv), is endemic in more than 100 countries. the lack of effective treatment of patients and the suboptimal efficacies of the tetravalent vaccine in trials highlight the urgent need to develop alternative strategies to lessen the burden of dengue fever.wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits the replication of the denv in the mosquito vector,aedes aegypti however, ...201626856916
a native wolbachia endosymbiont does not limit dengue virus infection in the mosquito aedes notoscriptus (diptera: culicidae).the endosymbiotic bacterium wolbachia pipientis infects many species of insects and has been transinfected into the mosquito aedes aegypti (l.), the primary vector of dengue virus (denv). recently, it has been shown that wolbachia blocks the replication and transmission of rna viruses, such as denv, in a number of mosquito species including ae. aegypti and aedes albopictus (skuse), which is naturally infected with wolbachia and considered a secondary vector for denv. the mosquito species aedes n ...201626721865
[spontaneous recovery from eosinophilic granuloma of bones of the cranial vault]. 20164849757
zika control through the bacterium wolbachia pipientis. 201627831765
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