| radical prostatectomy in the management of carcinoma of the prostate: probable causes of some therapeutic failures. | | 2016 | 5033963 |
| aging under glass. | | 2016 | 4929456 |
| [on a proposed law making obligatory the declaration of viral hepatitis and concerning disinfection]. | | 2016 | 4928868 |
| occlusion and prosthetic dentistry. | | 2016 | 4892616 |
| [primary and secondary plastic surgery after mandibular resection. (measures for restoration of normal facial structure and jaw function)]. | | 2016 | 4869337 |
| [spontaneous recovery from eosinophilic granuloma of bones of the cranial vault]. | | 2016 | 4849757 |
| actin in the alga, chara corallina. | | 2016 | 4835552 |
| [primary trigeminal neuralgia--odontologic points of view]. | | 2016 | 4766396 |
| editorial: investigative dermatology. | | 2016 | 4758728 |
| photochemistry of diazonium salts. i. synthesis of 4-fluoroimidazoles, 4-fluorohistamine, and 4-fluorohistidine. | | 2016 | 4730663 |
| detection of wolbachia in the tick ixodes ricinus is due to the presence of the hymenoptera endoparasitoid ixodiphagus hookeri. | the identification of micro-organisms carried by ticks is an important issue for human and animal health. in addition to their role as pathogen vectors, ticks are also the hosts for symbiotic bacteria whose impact on tick biology is poorly known. among these, the bacterium wolbachia pipientis has already been reported associated with ixodes ricinus and other tick species. however, the origins of wolbachia in ticks and their consequences on tick biology (known to be very diverse in invertebrates, ... | 2012 | 22292021 |
| selective whole genome amplification for resequencing target microbial species from complex natural samples. | population genomic analyses have demonstrated power to address major questions in evolutionary and molecular microbiology. collecting populations of genomes is hindered in many microbial species by the absence of a cost effective and practical method to collect ample quantities of sufficiently pure genomic dna for next-generation sequencing. here we present a simple method to amplify genomes of a target microbial species present in a complex, natural sample. the selective whole genome amplificat ... | 2014 | 25096321 |
| mosaic composition of riba and wspb genes flanking the virb8-d4 operon in the wolbachia supergroup b-strain, wstr. | the obligate intracellular bacterium, wolbachia pipientis (rickettsiales), is a widespread, vertically transmitted endosymbiont of filarial nematodes and arthropods. in insects, wolbachia modifies reproduction, and in mosquitoes, infection interferes with replication of arboviruses, bacteria and plasmodia. development of wolbachia as a tool to control pest insects will be facilitated by an understanding of molecular events that underlie genetic exchange between wolbachia strains. here, we used n ... | 2015 | 26400107 |
| the wolbachia wo bacteriophage proteome in the aedes albopictus c/wstr1 cell line: evidence for lytic activity? | wolbachia pipientis (rickettsiales), an obligate intracellular alphaproteobacterium in insects, manipulates host reproduction to maximize invasion of uninfected insect populations. modification of host population structure has potential applications for control of pest species, particularly if wolbachia can be maintained, manipulated, and genetically engineered in vitro. although wolbachia maintains an obligate mutualism with genome stability in nematodes, arthropods can be co-infected with dist ... | 2015 | 26427709 |
| supergroup c wolbachia, mutualist symbionts of filarial nematodes, have a distinct genome structure. | wolbachia pipientis is possibly the most widespread endosymbiont of arthropods and nematodes. while all wolbachia strains have historically been defined as a single species, 16 monophyletic clusters of diversity (called supergroups) have been described. different supergroups have distinct host ranges and symbiotic relationships, ranging from mutualism to reproductive manipulation. in filarial nematodes, which include parasites responsible for major diseases of humans (such as onchocerca volvulus ... | 2015 | 26631376 |
| comparative analysis of wolbachia genomes reveals streamlining and divergence of minimalist two-component systems. | two-component regulatory systems are commonly used by bacteria to coordinate intracellular responses with environmental cues. these systems are composed of functional protein pairs consisting of a sensor histidine kinase and cognate response regulator. in contrast to the well-studied caulobacter crescentus system, which carries dozens of these pairs, the streamlined bacterial endosymbiont wolbachia pipientis encodes only two pairs: ccka/ctra and plec/pled. here, we used bioinformatic tools to co ... | 2015 | 25809075 |
| proteomic profiling of a robust wolbachia infection in an aedes albopictus mosquito cell line. | wolbachia pipientis, a widespread vertically transmitted intracellular bacterium, provides a tool for insect control through manipulation of host-microbe interactions. we report proteomic characterization of wstr, a wolbachia strain associated with a strong cytoplasmic incompatibility phenotype in its native host, laodelphax striatellus. in the aedes albopictus c/wstr1 mosquito cell line, wstr maintains a robust, persistent infection. ms/ms analyses of gel bands revealed a protein 'footprint' do ... | 2014 | 25155417 |
| experimental evolution reveals habitat-specific fitness dynamics among wolbachia clades in drosophila melanogaster. | the diversity and infection dynamics of the endosymbiont wolbachia can be influenced by many factors, such as transmission rate, cytoplasmic incompatibility, environment, selection and genetic drift. the interplay of these factors in natural populations can result in heterogeneous infection patterns with substantial differences between populations and strains. the causes of these heterogeneities are not yet understood, partly due to the complexity of natural environments. we present experimental ... | 2014 | 24387805 |
| comparative genomics suggests an independent origin of cytoplasmic incompatibility in cardinium hertigii. | terrestrial arthropods are commonly infected with maternally inherited bacterial symbionts that cause cytoplasmic incompatibility (ci). in ci, the outcome of crosses between symbiont-infected males and uninfected females is reproductive failure, increasing the relative fitness of infected females and leading to spread of the symbiont in the host population. ci symbionts have profound impacts on host genetic structure and ecology and may lead to speciation and the rapid evolution of sex determina ... | 2012 | 23133394 |
| presence of extensive wolbachia symbiont insertions discovered in the genome of its host glossina morsitans morsitans. | tsetse flies (glossina spp.) are the cyclical vectors of trypanosoma spp., which are unicellular parasites responsible for multiple diseases, including nagana in livestock and sleeping sickness in humans in africa. glossina species, including glossina morsitans morsitans (gmm), for which the whole genome sequence (wgs) is now available, have established symbiotic associations with three endosymbionts: wigglesworthia glossinidia, sodalis glossinidius and wolbachia pipientis (wolbachia). the prese ... | 2014 | 24763283 |
| genomes of candidatus wolbachia bourtzisii wdaca and candidatus wolbachia pipientis wdacb from the cochineal insect dactylopius coccus (hemiptera: dactylopiidae). | dactylopius species, known as cochineal insects, are the source of the carminic acid dye used worldwide. the presence of two wolbachia strains in dactylopius coccus from mexico was revealed by pcr amplification of wsp and sequencing of 16s rrna genes. a metagenome analysis recovered the genome sequences of candidatus wolbachia bourtzisii wdaca (supergroup a) and candidatus wolbachia pipientis wdacb (supergroup b). genome read coverage, as well as 16s rrna clone sequencing, revealed that wdacb wa ... | 2016 | 27543297 |
| draft genome sequence of the male-killing wolbachia strain wbol1 reveals recent horizontal gene transfers from diverse sources. | the endosymbiont wolbachia pipientis causes diverse and sometimes dramatic phenotypes in its invertebrate hosts. four wolbachia strains sequenced to date indicate that the constitution of the genome is dynamic, but these strains are quite divergent and do not allow resolution of genome diversification over shorter time periods. we have sequenced the genome of the strain wbol1-b, found in the butterfly hypolimnas bolina, which kills the male offspring of infected hosts during embyronic developmen ... | 2013 | 23324387 |
| the small interfering rna pathway is not essential for wolbachia-mediated antiviral protection in drosophila melanogaster. | wolbachia pipientis delays rna virus-induced mortality in drosophila spp. we investigated whether wolbachia-mediated protection was dependent on the small interfering rna (sirna) pathway, a key antiviral defense. compared to wolbachia-free flies, virus-induced mortality was delayed in wolbachia-infected flies with loss-of-function of sirna pathway components, indicating that wolbachia-mediated protection functions in the absence of the canonical sirna pathway. | 2012 | 22798369 |
| no effect of wolbachia on resistance to intracellular infection by pathogenic bacteria in drosophila melanogaster. | multiple studies have shown that infection with the endosymbiotic bacterium wolbachia pipientis confers drosophila melanogaster and other insects with resistance to infection by rna viruses. studies investigating whether wolbachia infection induces the immune system or confers protection against secondary bacterial infection have not shown any effect. these studies, however, have emphasized resistance against extracellular pathogens. since wolbachia lives inside the host cell, we hypothesized th ... | 2012 | 22808174 |
| the impact of wolbachia on virus infection in mosquitoes. | mosquito-borne viruses such as dengue, west nile and chikungunya viruses cause significant morbidity and mortality in human populations. since current methods are not sufficient to control disease occurrence, novel methods to control transmission of arboviruses would be beneficial. recent studies have shown that virus infection and transmission in insects can be impeded by co-infection with the bacterium wolbachia pipientis. wolbachia is a maternally inherited endosymbiont that is commonly found ... | 2015 | 26556361 |
| drosophila melanogaster as a model organism for bluetongue virus replication and tropism. | bluetongue virus (btv) is the etiological agent of bluetongue (bt), a hemorrhagic disease of ruminants that can cause high levels of morbidity and mortality. btv is an arbovirus transmitted between its ruminant hosts by culicoides biting midges (diptera: ceratopogonidae). recently, europe has experienced some of the largest bt outbreaks ever recorded, including areas with no known history of the disease, leading to unprecedented economic and animal welfare issues. the current lack of genomic res ... | 2012 | 22674991 |
| wolbachia reduces the transmission potential of dengue-infected aedes aegypti. | dengue viruses (denv) are the causative agents of dengue, the world's most prevalent arthropod-borne disease with around 40% of the world's population at risk of infection annually. wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits replication of the virus in the mosquito. the wolbachia strain wmel, which has been introduced into the mosquito vector, aedes aegypti, has been shown to invade and spread to near fix ... | 2015 | 26115104 |
| downregulation of aedes aegypti chromodomain helicase dna binding protein 7/kismet by wolbachia and its effect on dengue virus replication. | dengue virus (denv) is a mosquito-transmitted virus imposing a significant burden on human health around the world. since current control strategies are not sufficient, there is an urgent need to find alternative methods to control denv transmission. it has been demonstrated that introduction of wolbachia pipientis in aedes aegypti mosquitoes can impede denv transmission with the mechanism(s) not fully understood. recently, a number of studies have found the involvement of chromodomain dna bindi ... | 2016 | 27827425 |
| wolbachia uses a host microrna to regulate transcripts of a methyltransferase, contributing to dengue virus inhibition in aedes aegypti. | the endosymbiont wolbachia is common among insects and known for the reproductive manipulations it exerts on hosts as well as inhibition of virus replication in their hosts. recently, we showed that wolbachia uses host micrornas to manipulate host gene expression for its efficient maintenance in the dengue mosquito vector, aedes aegypti. cytosine methylation is mediated by a group of proteins called dna (cytosine-5) methyltransferases, which are structurally and functionally conserved from proka ... | 2013 | 23733960 |
| diversity of wolbachia pipientis strain wpip in a genetically admixtured, above-ground culex pipiens (diptera: culicidae) population: association with form molestus ancestry and host selection patterns. | analysis of molecular genetic diversity in nine marker regions of five genes within the bacteriophage wo genomic region revealed high diversity of the wolbachia pipentis strain wpip in a population of culex pipiens l. sampled in metropolitan chicago, il. from 166 blood fed females, 50 distinct genetic profiles of wpip were identified. rarefaction analysis suggested a maximum of 110 profiles out of a possible 512 predicted by combinations of the nine markers. a rank-abundance curve showed that fe ... | 0 | 22679853 |
| genetic diversity of culex pipiens mosquitoes in distinct populations from europe: contribution of cx. quinquefasciatus in mediterranean populations. | mosquitoes of the culex pipiens complex are cosmopolitan, and important vectors of neglected tropical diseases, such as arbovirosis and lymphatic filariasis. among the complex taxa, cx. pipiens (with two forms pipiens and molestus) and cx. quinquefasciatus are the most ubiquitous mosquitoes in temperate and tropical regions respectively. mosquitoes of this taxa lack of morphological differences between females, but have frank behavioral and physiological differences and have different trophic pr ... | 2016 | 26818097 |
| the α-proteobacteria wolbachia pipientis protein disulfide machinery has a regulatory mechanism absent in γ-proteobacteria. | the α-proteobacterium wolbachia pipientis infects more than 65% of insect species worldwide and manipulates the host reproductive machinery to enable its own survival. it can live in mutualistic relationships with hosts that cause human disease, including mosquitoes that carry the dengue virus. like many other bacteria, wolbachia contains disulfide bond forming (dsb) proteins that introduce disulfide bonds into secreted effector proteins. the genome of the wolbachia strain wmel encodes two dsba- ... | 2013 | 24282596 |
| french invasive asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to vietnamese autochthonous relatives. | the asian tiger mosquito aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. originating from asia, it has invaded a wide range of eco-climatic regions worldwide. the insect-associated microbiota is now recognized to play a significant role in host biology. while genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. to address this su ... | 2015 | 26441903 |
| wolbachia: can we save lives with a great pandemic? | wolbachia pipientis is the most common bacterial infection in the animal world and wields a vast influence on invertebrate reproduction, sex determination, speciation, and behavior worldwide. these avenues of research have made seminal gains, including the latest use of wolbachia to alter mosquito populations and a strengthened focus on using anti-wolbachia therapies against filarial nematode infections. this work is further bolstered by a more refined knowledge of wolbachia biology spanning mec ... | 2013 | 23845310 |
| dietary cholesterol modulates pathogen blocking by wolbachia. | the bacterial endosymbiont wolbachia pipientis protects its hosts from a range of pathogens by limiting their ability to form infections inside the insect. this "pathogen blocking" could be explained by innate immune priming by the symbiont, competition for host-derived resources between pathogens and wolbachia, or the direct modification of the cell or cellular environment by wolbachia. recent comparative work in drosophila and the mosquito aedes aegypti has shown that an immune response is not ... | 2013 | 23825950 |
| near-infrared spectroscopy, a rapid method for predicting the age of male and female wild-type and wolbachia infected aedes aegypti. | estimating the age distribution of mosquito populations is crucial for assessing their capacity to transmit disease and for evaluating the efficacy of available vector control programs. this study reports on the capacity of the near-infrared spectroscopy (nirs) technique to rapidly predict the ages of the principal dengue and zika vector, aedes aegypti. the age of wild-type males and females, and males and females infected with wmel and wmelpop strains of wolbachia pipientis were characterized u ... | 2016 | 27768689 |
| decapitation improves detection of wolbachia pipientis (rickettsiales: anaplasmataceae) in culex pipiens (diptera: culicidae) mosquitoes by the polymerase chain reaction. | polymerase chain reaction (pcr) is often used to detect microorganisms, pathogens, or both, including the reproductive parasite wolbachia pipientis (rickettsiales: anaplasmataceae), in mosquitoes. natural populations of culex pipiens l. (diptera: culicidae) mosquitoes are infected with one or more strains of w. pipientis, and crosses between mosquitoes harboring different wolbachia strains provide one of the best-known examples of cytoplasmic incompatibililty (ci). when we used pcr to monitor wo ... | 0 | 23025192 |
| detection of wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis. | individual, naturally occurring phlebotomus mongolensis and phlebotomus caucasicus from iran were screened for infections with the maternally inherited intracellular rickettsia-like bacterium wolbachia pipientis via targeting a major surface protein gene (wsp). the main objective of this study was to determine if w. pipientis could be detected in these species. the sandflies were screened using polymerase chain reaction to amplify a fragment of the wolbachia surface protein gene. the obtained se ... | 0 | 23828002 |
| detection of a new strain of wolbachia pipientis in phlebotomus perfiliewi transcaucasicus, a potential vector of visceral leishmaniasis in north west of iran, by targeting the major surface protein gene. | wolbachia pipientis is maternally inherited endoparasitic bacterium belonging to the α-proteobacteria, infecting 20-75% of all insect species including sand flies. the wolbachia surface protein (wsp) was employed as an appropriate marker for strain typing. the objective of our research was to find the possibility of detection of w. pipientis in phlebotomus perfiliewi transcaucasicus. | 2013 | 23785694 |
| transcriptional regulation of culex pipiens mosquitoes by wolbachia influences cytoplasmic incompatibility. | cytoplasmic incompatibility (ci) induced by the endosymbiont wolbachia pipientis causes complex patterns of crossing sterility between populations of the culex pipiens group of mosquitoes. the molecular basis of the phenotype is yet to be defined. in order to investigate what host changes may underlie ci at the molecular level, we examined the transcription of a homolog of the drosophila melanogaster gene grauzone that encodes a zinc finger protein and acts as a regulator of female meiosis, in w ... | 2013 | 24204251 |
| tsetse-wolbachia symbiosis: comes of age and has great potential for pest and disease control. | tsetse flies (diptera: glossinidae) are the sole vectors of african trypanosomes, the causative agent of sleeping sickness in human and nagana in animals. like most eukaryotic organisms, glossina species have established symbiotic associations with bacteria. three main symbiotic bacteria have been found in tsetse flies: wigglesworthia glossinidia, an obligate symbiotic bacterium, the secondary endosymbiont sodalis glossinidius and the reproductive symbiont wolbachia pipientis. in the present rev ... | 2012 | 22835476 |
| the modulation of the symbiont/host interaction between wolbachia pipientis and aedes fluviatilis embryos by glycogen metabolism. | wolbachia pipientis, a maternally transmitted bacterium that colonizes arthropods, may affect the general aspects of insect physiology, particularly reproduction. wolbachia is a natural endosymbiont of aedes fluviatilis, whose effects in embryogenesis and reproduction have not been addressed so far. in this context, we investigated the correlation between glucose metabolism and morphological alterations during a. fluviatilis embryo development in wolbachia-positive (w+) and wolbachia-negative (w ... | 2014 | 24926801 |
| the influence of larval competition on brazilian wolbachia-infected aedes aegypti mosquitoes. | with field releases starting in brazil, particular interest must be given to understanding how the endosymbiotic bacterium wolbachia pipientis affects aedes aegypti mosquitoes with a brazilian genetic background. currently, there is limited information on how the bacterium affects phenotypic traits such as larval development rate, metabolic reserves and morphometric parameters in ae. aegypti. here, we analyze for the first time, the effect of wolbachia on these key phenotypes and consider how th ... | 2016 | 27183820 |
| wolbachia blocks currently circulating zika virus isolates in brazilian aedes aegypti mosquitoes. | the recent association of zika virus with cases of microcephaly has sparked a global health crisis and highlighted the need for mechanisms to combat the zika vector, aedes aegypti mosquitoes. wolbachia pipientis, a bacterial endosymbiont of insect, has recently garnered attention as a mechanism for arbovirus control. here we report that aedes aegypti harboring wolbachia are highly resistant to infection with two currently circulating zika virus isolates from the recent brazilian epidemic. wolbac ... | 2016 | 27156023 |
| dynamics of wolbachia pipientis gene expression across the drosophila melanogaster life cycle. | symbiotic interactions between microbes and their multicellular hosts have manifold biological consequences. to better understand how bacteria maintain symbiotic associations with animal hosts, we analyzed genome-wide gene expression for the endosymbiotic α-proteobacteria wolbachia pipientis across the entire life cycle of drosophila melanogaster. we found that the majority of wolbachia genes are expressed stably across the d. melanogaster life cycle, but that 7.8% of wolbachia genes exhibit rob ... | 2015 | 26497146 |
| the expression of one ankyrin pk2 allele of the wo prophage is correlated with the wolbachia feminizing effect in isopods. | the maternally inherited α-proteobacteria wolbachia pipientis is an obligate endosymbiont of nematodes and arthropods, in which they induce a variety of reproductive alterations, including cytoplasmic incompatibility (ci) and feminization. the genome of the feminizing wvulc wolbachia strain harboured by the isopod armadillidium vulgare has been sequenced and is now at the final assembly step. it contains an unusually high number of ankyrin motif-containing genes, two of which are homologous to t ... | 2012 | 22497736 |
| detection of the wolbachia protein wpip0282 in mosquito spermathecae: implications for cytoplasmic incompatibility. | cytoplasmic incompatibility (ci) is a conditional sterility induced by the bacterium wolbachia pipientis that infects reproductive tissues in many arthropods. although ci provides a potential tool to control insect vectors of arthropod-borne diseases, the molecular basis for ci induction is unknown. we hypothesized that a wolbachia-encoded, ci-inducing factor would be enriched in sperm recovered from spermathecae of female mosquitoes. using sds-page and mass spectrometry, we detected peptides fr ... | 2013 | 23856508 |
| the complexity of virus systems: the case of endosymbionts. | host-microbe symbioses involving bacterial endosymbionts comprise some of the most intimate and long-lasting interactions on the planet. while restricted gene flow might be expected due to their intracellular lifestyle, many endosymbionts, especially those that switch hosts, are rampant with mobile dna and bacteriophages. one endosymbiont, wolbachia pipientis, infects a vast number of arthropod and nematode species and often has a significant portion of its genome dedicated to prophage sequences ... | 2012 | 22609369 |
| risk associated with the release of wolbachia-infected aedes aegypti mosquitoes into the environment in an effort to control dengue. | in an effort to eliminate dengue, a successful technology was developed with the stable introduction of the obligate intracellular bacteria wolbachia pipientis into the mosquito aedes aegypti to reduce its ability to transmit dengue fever due to life shortening and inhibition of viral replication effects. an analysis of risk was required before considering release of the modified mosquito into the environment. | 2016 | 27047911 |
| application of wmelpop wolbachia strain to crash local populations of aedes aegypti. | the endosymbiotic bacteria wolbachia pipientis (wmel strain) has been successfully established in several populations of aedes aegypti, the primary dengue vector. the virulent wolbachia strain wmelpop is known to cause several pathological impacts (increased egg mortality, life shortening, etc.) reducing overall fitness in the mosquito ae. aegypti. increased egg mortality could substantially reduce egg banks in areas with a lengthy monsoonal dry season, and be employed to eliminate local populat ... | 2015 | 26204449 |
| wolbachia-based dengue virus inhibition is not tissue-specific in aedes aegypti. | dengue fever, caused by the dengue virus (denv), is now the most common arbovirus transmitted disease globally. one novel approach to control denv is to use the endosymbiotic bacterium, wolbachia pipientis, to limit denv replication inside the primary mosquito vector, aedes aegypti. wolbachia that is naturally present in a range of insects reduces the capacity for viruses, bacteria, parasites and fungi to replicate inside insects. wolbachia's mode of action is not well understood but may involve ... | 2016 | 27855218 |
| genomic evolution of the pathogenic wolbachia strain, wmelpop. | most strains of the widespread endosymbiotic bacterium wolbachia pipientis are benign or behave as reproductive parasites. the pathogenic strain wmelpop is a striking exception, however: it overreplicates in its insect hosts and causes severe life shortening. the mechanism of this pathogenesis is currently unknown. we have sequenced the genomes of three variants of wmelpop and of the closely related nonpathogenic strain wmelcs. we show that the genomes of wmelcs and wmelpop appear to be identica ... | 0 | 24190075 |
| rapid and non-destructive detection and identification of two strains of wolbachia in aedes aegypti by near-infrared spectroscopy. | the release of wolbachia infected mosquitoes is likely to form a key component of disease control strategies in the near future. we investigated the potential of using near-infrared spectroscopy (nirs) to simultaneously detect and identify two strains of wolbachia pipientis (wmelpop and wmel) in male and female laboratory-reared aedes aegypti mosquitoes. our aim is to find faster, cheaper alternatives for monitoring those releases than the molecular diagnostic techniques that are currently in us ... | 2016 | 27362709 |
| establishment of a wolbachia superinfection in aedes aegypti mosquitoes as a potential approach for future resistance management. | wolbachia pipientis is an endosymbiotic bacterium estimated to chronically infect between 40-75% of all arthropod species. aedes aegypti, the principle mosquito vector of dengue virus (denv), is not a natural host of wolbachia. the transinfection of wolbachia strains such as walbb, wmel and wmelpop-cla into ae. aegypti has been shown to significantly reduce the vector competence of this mosquito for a range of human pathogens in the laboratory. this has led to wmel-transinfected ae. aegypti curr ... | 2016 | 26891349 |
| adult survivorship of the dengue mosquito aedes aegypti varies seasonally in central vietnam. | the survival characteristics of the mosquito aedes aegypti affect transmission rates of dengue because transmission requires infected mosquitoes to survive long enough for the virus to infect the salivary glands. mosquito survival is assumed to be high in tropical, dengue endemic, countries like vietnam. however, the survival rates of wild populations of mosquitoes are seldom measured due the difficulty of predicting mosquito age. hon mieu island in central vietnam is the site of a pilot release ... | 2014 | 24551251 |
| discovery of putative small non-coding rnas from the obligate intracellular bacterium wolbachia pipientis. | wolbachia pipientis is an endosymbiotic bacterium that induces a wide range of effects in its insect hosts, including manipulation of reproduction and protection against pathogens. little is known of the molecular mechanisms underlying the insect-wolbachia interaction, though it is likely to be mediated via the secretion of proteins or other factors. there is an increasing amount of evidence that bacteria regulate many cellular processes, including secretion of virulence factors, using small non ... | 2015 | 25739023 |
| the relative importance of dna methylation and dnmt2-mediated epigenetic regulation on wolbachia densities and cytoplasmic incompatibility. | wolbachia pipientis is a worldwide bacterial parasite of arthropods that infects germline cells and manipulates host reproduction to increase the ratio of infected females, the transmitting sex of the bacteria. the most common reproductive manipulation, cytoplasmic incompatibility (ci), is expressed as embryonic death in crosses between infected males and uninfected females. specifically, wolbachia modify developing sperm in the testes by unknown means to cause a post-fertilization disruption of ... | 2014 | 25538866 |
| wolbachia-induced aae-mir-12 mirna negatively regulates the expression of mct1 and mcm6 genes in wolbachia-infected mosquito cell line. | best recognized for its role in manipulating host reproduction, the parasitic gram-negative wolbachia pipientis is known to colonize a wide range of invertebrates. the endosymbiotic bacterium has recently been shown to cause a life-shortening effect as well as inhibiting replication of arboviruses in aedes aegypti; although the molecular mechanisms behind these effects are largely unknown. micrornas (mirnas) have been determined to have a wide range of roles in regulating gene expression in euka ... | 2012 | 23166816 |
| intercommunity effects on microbiome and gpsghv density regulation in tsetse flies. | tsetse flies have a highly regulated and defined microbial fauna made of 3 bacterial symbionts (obligate wigglesworthia glossinidia, commensal sodalis glossinidius and parasitic wolbachia pipientis) in addition to a dna virus (glossina pallidipes salivary gland hypertrophy virus, gpsghv). it has been possible to rear flies in the absence of either wigglesworthia or in totally aposymbiotic state by dietary supplementation of tsetse's bloodmeal. in the absence of wigglesworthia, tsetse females are ... | 2012 | 22874746 |
| pyrosequencing 16s rrna genes of bacteria associated with wild tiger mosquito aedes albopictus: a pilot study. | the asian tiger mosquito aedes (stegomya) albopictus is an invasive species that has spread across the world in the last two decades, showing a great capacity to adapt to contrasting climates and environments. while demonstrated in many insects, the contribution of bacterial symbionts in aedes ecology is a challenging aspect that needs to be investigated. also some bacterial species have already been identified in ae. albopictus using classical methods, but a more accurate survey of mosquito-ass ... | 2014 | 24860790 |
| identification and characterization of a candidate wolbachia pipientis type iv effector that interacts with the actin cytoskeleton. | many bacteria live as intracellular symbionts, causing persistent infections within insects. one extraordinarily common infection is that of wolbachia pipientis, which infects 40% of insect species and induces reproductive effects. the bacteria are passed from generation to generation both vertically (through the oocyte) and horizontally (by environmental transmission). maintenance of the infection within drosophila melanogaster is sensitive to the regulation of actin, as wolbachia inefficiently ... | 2016 | 27381293 |
| wolbachia utilize host actin for efficient maternal transmission in drosophila melanogaster. | wolbachia pipientis is a ubiquitous, maternally transmitted bacterium that infects the germline of insect hosts. estimates are that wolbachia infect nearly 40% of insect species on the planet, making it the most prevalent infection on earth. the bacterium, infamous for the reproductive phenotypes it induces in arthropod hosts, has risen to recent prominence due to its use in vector control. wolbachia infection prevents the colonization of vectors by rna viruses, including drosophila c virus and ... | 2015 | 25906062 |
| wolbachia, sodalis and trypanosome co-infections in natural populations of glossina austeni and glossina pallidipes. | tsetse flies harbor at least three bacterial symbionts: wigglesworthia glossinidia, wolbachia pipientis and sodalis glossinidius. wigglesworthia and sodalis reside in the gut in close association with trypanosomes and may influence establishment and development of midgut parasite infections. wolbachia has been shown to induce reproductive effects in infected tsetse. this study was conducted to determine the prevalence of these endosymbionts in natural populations of g. austeni and g. pallidipes ... | 2013 | 23924682 |
| the oxidizing agent, paraquat, is more toxic to wolbachia than to mosquito host cells. | cultured cells provide an important in vitro system for examining metabolic interactions between the intracellular bacterium, wolbachia pipientis, and its insect hosts. to test whether wolbachia-associated changes in antioxidant activities could provide a tool to select for infected cells, we tested the effects of paraquat (pq) on aedes albopictus mosquito cells. like mammalian cells, mosquito cells tolerate pq over a wide range of concentrations, and for considerable lengths of time, depending ... | 2013 | 23719839 |
| evaluation of microbial communities and symbionts in ixodes ricinus and ungulate hosts (cervus elaphus and ovis aries) from shared habitats on the west coast of norway. | recent reports suggest a potential for transmission of a newly discovered rickettsial endosymbiont, midichloria mitochondrii, to animals and humans from feeding ticks (ixodes ricinus). using molecular methods; i. ricinus, sheep and red deer in anaplasma phagocytophilum-endemic areas of norway, were examined to see if they were infected by m. mitochondrii or related organisms like wolbachia pipientis and rickettsia spp. a total of 532 ticks collected from pastures, 76 blood samples from grazing l ... | 2014 | 25132534 |
| effect of wolbachia on insecticide susceptibility in lines of aedes aegypti. | two stable infections of wolbachia pipientis, wmelpop and wmel, now established in aedes aegypti, are being used in a biocontrol program to suppress the transmission of dengue. any effects of wolbachia infection on insecticide resistance of mosquitoes may undermine the success of this program. bioassays of ae. aegypti were conducted to test for differences in response to insecticides between wolbachia infected (wmelpop, wmel) and uninfected lines. insecticides screened were bifenthrin, the pyret ... | 2013 | 23149015 |
| the rnai pathway plays a small part in wolbachia-mediated blocking of dengue virus in mosquito cells. | wolbachia pipientis is an insect endosymbiont known to limit the replication of viruses including dengue and zika in their primary mosquito vector, aedes aegypti. wolbachia is being released into mosquito populations globally in a bid to control the diseases caused by these viruses. it is theorized that wolbachia's priming of the insect immune system may confer protection against subsequent viral infection. other hypotheses posit a role for competition between wolbachia and viruses for host cell ... | 2017 | 28262718 |
| the effect of temperature on wolbachia-mediated dengue virus blocking in aedes aegypti. | dengue fever, caused by dengue virus (denv), is endemic in more than 100 countries. the lack of effective treatment of patients and the suboptimal efficacies of the tetravalent vaccine in trials highlight the urgent need to develop alternative strategies to lessen the burden of dengue fever.wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits the replication of the denv in the mosquito vector,aedes aegypti however, ... | 2016 | 26856916 |
| a native wolbachia endosymbiont does not limit dengue virus infection in the mosquito aedes notoscriptus (diptera: culicidae). | the endosymbiotic bacterium wolbachia pipientis infects many species of insects and has been transinfected into the mosquito aedes aegypti (l.), the primary vector of dengue virus (denv). recently, it has been shown that wolbachia blocks the replication and transmission of rna viruses, such as denv, in a number of mosquito species including ae. aegypti and aedes albopictus (skuse), which is naturally infected with wolbachia and considered a secondary vector for denv. the mosquito species aedes n ... | 2016 | 26721865 |
| wolbachia pipientis: a potential candidate for combating and eradicating dengue epidemics in pakistan. | dengue virus syndrome is an emerging global health challenge which is endemic in tropical countries like pakistan. in recent years dengue incidences have increased considerably in different areas of pakistan with more sever impacts on urban and peri-urban populations. this review is an effort to highlight the changing epidemiology of dengue fever, role of government of pakistan in disease management and control using preventive and community based approaches in the region. moreover, there is an ... | 2015 | 26706669 |
| effects of mimosine on wolbachia in mosquito cells: cell cycle suppression reduces bacterial abundance. | the plant allelochemical l-mimosine (β-[n-(3-hydroxy-4-pyridone)]-α-aminopropionic acid; leucenol) resembles the nonessential amino acid, tyrosine. because the obligate intracellular alphaproteobacterium, wolbachia pipientis, metabolizes amino acids derived from host cells, the effects of mimosine on infected and uninfected mosquito cells were investigated. the ec50 for mimosine was 6-7 μm with aedes albopictus c7-10 and c/wstr cell lines, and was not influenced by infection status. mosquito cel ... | 2015 | 26019119 |
| molecular detection of wolbachia pipientis in natural populations of mosquito vectors of dirofilaria immitis from continental portugal: first detection in culex theileri. | wolbachia pipientis (rickettsiales: rickettsiaceae) protects mosquitoes from infections with arboviruses and parasites. however, the effect of its co-infection on vector competence for dirofilaria immitis (spirurida: onchocercidae) in the wild has not been investigated. this study aimed to screen vectors of d. immitis for wpip, to characterize these, and to investigate a possible association between the occurrence of w. pipientis and that of the nematode. the presence of w. pipientis was assesse ... | 2016 | 27279553 |
| detection of wolbachia dna in blood for diagnosing filaria-associated syndromes in cats. | a fundamental role for the endosymbiotic bacteria wolbachia pipientis in the pathogenesis of dirofilaria immitis infections has emerged in recent years. diagnostic opportunities arising from this breakthrough have not yet been fully exploited. this study was aimed at developing conventional and real-time pcr assays to carry out a molecular survey in a convenience sample of cats living in an area where d. immitis is endemic and to evaluate the detection of bacterial dna in blood as a surrogate as ... | 2012 | 22649020 |
| a rare human case of dirofilaria repens infection in the subcutaneous posterior thorax with molecular identification. | the emergence of dirofilarial infections in asia including vietnam is a clinically significant threat to the community. we here report a rare case of subcutaneous dirofilaria repens infection on the posterior thoracic wall in a young woman presenting a painful, itchy, and palpable nodule. the adult worm was identified by mitochondrial cox1 and nuclear its-2 sequence determination. the diagnosis was additionally confirmed by 16s rrna sequencing of the endosymbiont wolbachia pipientis commonly co- ... | 2015 | 26174827 |
| somatic wolbachia (rickettsiales: rickettsiaceae) levels in culex quinquefasciatus and culex pipiens (diptera: culicidae) and resistance to west nile virus infection. | the endosymbiotic bacteria wolbachia pipientis hertig infects a wide variety of insect species and can increase viral resistance in its host. wolbachia naturally infects culex quinquefasciatus say and culex pipiens l. mosquitoes, both vectors of west nile virus (wnv). we recently demonstrated that wolbachia infection of cx. quinquefasciatus laboratory strain ben95 increases host resistance to wnv infection, reducing vector competence. this observation raised the possibility that wolbachia could ... | 2014 | 24605469 |
| incomplete removal of wolbachia with tetracycline has two-edged reproductive effects in the thelytokous wasp encarsia formosa (hymenoptera: aphelinidae). | wolbachia pipientis are intracellular endosymbionts that induce parthenogenesis in the parasitoid encarsia formosa. previous studies that focused on effects of wolbachia on the wasp usually used tetracycline to remove wolbachia without concern for the joint influences of tetracycline and wolbachia. here we treated the wasps (f0 lines) with tetracycline to produce offspring (f1 lines) which were not fed tetracycline to avoid antibiotic influence. the quantitative data and fluorescence in situ hyb ... | 2017 | 28266601 |
| the re-emerging arboviral threat: hidden enemies: the emergence of obscure arboviral diseases, and the potential use of wolbachia in their control. | mayaro, oropouche, and o'nyong-nyong share many traits with more prominent arboviruses, like dengue and yellow fever, chikungunya, and zika. these include severe clinical symptoms, multiple animal hosts, and widespread vector species living in close proximity to human habitats, all of which constitute significant risk factors for more frequent outbreaks in the future, greatly increasing the potential of these hidden enemies to follow zika and become the next wave of global arboviral threats. cri ... | 2017 | 28026036 |
| zika control through the bacterium wolbachia pipientis. | | 2016 | 27831765 |
| wolbachia endosymbionts modify drosophila ovary protein levels in a context-dependent manner. | endosymbiosis is a unique form of interaction between organisms, with one organism dwelling inside the other. one of the most widespread endosymbionts is wolbachia pipientis, a maternally transmitted bacterium carried by insects, crustaceans, mites, and filarial nematodes. although candidate proteins that contribute to maternal transmission have been identified, the molecular basis for maternal wolbachia transmission remains largely unknown. to investigate transmission-related processes in respo ... | 2016 | 27342560 |
| comparative genomics of two closely related wolbachia with different reproductive effects on hosts. | wolbachia pipientis are obligate intracellular bacteria commonly found in many arthropods. they can induce various reproductive alterations in hosts, including cytoplasmic incompatibility, male-killing, feminization, and parthenogenetic development, and can provide host protection against some viruses and other pathogens. wolbachia differ from many other primary endosymbionts in arthropods because they undergo frequent horizontal transmission between hosts and are well known for an abundance of ... | 2016 | 27189996 |
| [drosophila melanogaster cell culture as an experimental model to study recombination in wolbachia pipientis]. | wolbachiapipientis is an obligate intracellular endosymbiont that commonly infects arthropods. comparative genomic studies of wolbachia reveal traces of numerous events of intergenic and intragenic recombination. the molecular mechanisms of recombination in wolbachia are not currently known. we conducted experimental verification of the possibility of recombination of two strains of wolbachia: wmel and wri, after using these strains for double infection of the dm2008wb1 (d. melanogaster) cell cu ... | 2015 | 27055293 |
| a response to lindsey et al. "wolbachia pipientis should not be split into multiple species: a response to ramírez-puebla et al.". | | 2016 | 27033416 |
| wolbachia pipientis should not be split into multiple species: a response to ramírez-puebla et al., "species in wolbachia? proposal for the designation of 'candidatus wolbachia bourtzisii', 'candidatus wolbachia onchocercicola', 'candidatus wolbachia blaxteri', 'candidatus wolbachia brugii', 'candidatus wolbachia taylori', 'candidatus wolbachia collembolicola' and 'candidatus wolbachia multihospitum' for the different species within wolbachia supergroups". | | 2016 | 27021523 |
| [genotypic diversity of wolbachia pipientis in native and invasive harmonia axyridis pall., 1773 (coleoptera, coccinellidae) populations]. | the distribution and variability of reproductive symbiotic wolbachia pipientis bacteria were studied in seven native and six invasive h. axyridis populations. wolbachia-infected individuals were found in two invasive and two native populations. we demonstrated for the first time an infection of invasive h. axyridis populations with wolbachia. two new molecular forms of wolbachia were detected by a system of multilocus typing. the supergroup a wolbachia was found for the first time in h. axyridis ... | 2015 | 26601484 |
| the drosophila bag of marbles gene interacts genetically with wolbachia and shows female-specific effects of divergence. | many reproductive proteins from diverse taxa evolve rapidly and adaptively. these proteins are typically involved in late stages of reproduction such as sperm development and fertilization, and are more often functional in males than females. surprisingly, many germline stem cell (gsc) regulatory genes, which are essential for the earliest stages of reproduction, also evolve adaptively in drosophila. one example is the bag of marbles (bam) gene, which is required for gsc differentiation and germ ... | 2015 | 26291077 |
| population genomics of infectious and integrated wolbachia pipientis genomes in drosophila ananassae. | coevolution between drosophila and its endosymbiont wolbachia pipientis has many intriguing aspects. for example, drosophila ananassae hosts two forms of w. pipientis genomes: one being the infectious bacterial genome and the other integrated into the host nuclear genome. here, we characterize the infectious and integrated genomes of w. pipientis infecting d. ananassae (wana), by genome sequencing 15 strains of d. ananassae that have either the infectious or integrated wana genomes. results indi ... | 2015 | 26254486 |
| wolbachia infection in a natural parasitoid wasp population. | the maternally transmitted bacterium wolbachia pipientis is well known for spreading and persisting in insect populations through manipulation of the fitness of its host. here, we identify three new wolbachia pipientis strains, whho, whho2 and whho3, infecting hyposoter horticola, a specialist wasp parasitoid of the glanville fritillary butterfly. the whho strain (st435) infects about 50% of the individuals in the åland islands in finland, with a different infection rate in the two mitochondrial ... | 2015 | 26244782 |
| species in wolbachia? proposal for the designation of 'candidatus wolbachia bourtzisii', 'candidatus wolbachia onchocercicola', 'candidatus wolbachia blaxteri', 'candidatus wolbachia brugii', 'candidatus wolbachia taylori', 'candidatus wolbachia collembolicola' and 'candidatus wolbachia multihospitum' for the different species within wolbachia supergroups. | wolbachia are highly extended bacterial endosymbionts that infect arthropods and filarial nematodes and produce contrasting phenotypes on their hosts. wolbachia taxonomy has been understudied. currently, wolbachia strains are classified into phylogenetic supergroups. here we applied phylogenomic analyses to study wolbachia evolutionary relationships and examined metrics derived from their genome sequences such as average nucleotide identity (ani), in silico dna-dna hybridization (ddh), g+c conte ... | 2015 | 26189661 |
| [coevolution of symbiotic bacteria wolbachia and host mtdna in russian populations of the culex pipiens mosquito complex]. | the mitochondrial dna phylogenies of closely related forms of mosquitoes from the culex pipiens complex and of strains of the endosymbiotic bacteria wolbachia pipientis were compared. based on the cytochrome oxidase subunit i gene polymorphism, six mitochondrial haplotypes and four w. pipientis groups were discovered in mosquitoes from geographically remote populations. a strict correlation between the coi type and the type of w. pipientis proves the stable coinheritance and distribution of both ... | 2014 | 25739293 |
| strain-specific and pooled genome sequences for populations of drosophila melanogaster from three continents. | to contribute to our general understanding of the evolutionary forces that shape variation in genome sequences in nature, we have sequenced genomes from 50 isofemale lines and six pooled samples from populations of drosophila melanogaster on three continents. analysis of raw and reference-mapped reads indicates the quality of these genomic sequence data is very high. comparison of the predicted and experimentally-determined wolbachia infection status of these samples suggests that strain or samp ... | 2015 | 25717372 |
| passage of wolbachia pipientis through mutant drosophila melanogaster induces phenotypic and genomic changes. | wolbachia pipientis is a nearly ubiquitous, maternally transmitted bacterium that infects the germ line of insect hosts. estimates are that wolbachia infects 40 to 60% of insect species on the planet, making it one of the most prevalent infections on earth. however, we know surprisingly little about the molecular mechanisms used by wolbachia to infect its hosts. we passaged wolbachia through normally restrictive drosophila melanogaster hosts, bottlenecking wolbachia through stochastic segregatio ... | 2015 | 25452279 |
| [how to fight parasitic infectious diseases with bacteria. the case of wolbachia pipientis]. | in nature, no individual can live in isolation; hence, living organisms are forced to interact with each other. this necessity has led many organisms to establish heterogeneous relations to enhance their ability to adapt to the environment, thus acquiring evolutionary advantages. these relationships are sometimes so intense, that on the long term the organisms may lose their individual identity. an example of these associations is the endosymbiotic ones, where eukaryote organisms generally harbo ... | 2016 | 25354059 |
| using wolbachia-based release for suppression of aedes mosquitoes: insights from genetic data and population simulations. | a novel strategy for suppressing disease transmission by aedes aegypti, the main vector of dengue, uses releases of mosquitoes infected with the bacterium wolbachia pipientis. wolbachia are currently released to interfere with viral transmission, but there is also potential to use strains in mosquito suppression and elimination programs via the deleterious effects of the bacterium on the host. mosquito suppression depends on target areas being relatively isolated to prevent reinvasion and on loc ... | 2014 | 25154109 |
| the coevolutionary period of wolbachia pipientis infecting drosophila ananassae and its impact on the evolution of the host germline stem cell regulating genes. | the endosymbiotic bacteria wolbachia pipientis is known to infect a wide range of arthropod species yet less is known about the coevolutionary history it has with its hosts. evidence of highly identical w. pipientis strains in evolutionary divergent hosts suggests horizontal transfer between hosts. for example, drosophila ananassae is infected with a w. pipientis strain that is nearly identical in sequence to a strain that infects both d. simulans and d. suzukii, suggesting recent horizontal tra ... | 2014 | 24974378 |
| microsatellite and wolbachia analysis in rhagoletis cerasi natural populations: population structuring and multiple infections. | rhagoletis cerasi (diptera: tephritidae) is a major pest of sweet and sour cherries in europe and parts of asia. despite its economic significance, there is a lack of studies on the genetic structure of r. cerasi populations. elucidating the genetic structure of insects of economic importance is crucial for developing phenological-predictive models and environmental friendly control methods. all natural populations of r. cerasi have been found to harbor the endosymbiont wolbachia pipientis, whic ... | 2014 | 24963388 |
| what can symbiont titres tell us about co-evolution of wolbachia and their host? | there is a long-standing prediction that associations with vertically transmitted symbionts evolve towards maximisation of host reproductive success, eventually leading to mutualist symbiosis and coadaptation. under this scenario, the regulation of symbiont titres in host tissues would be expected to be greater when partners have coevolved for a long time than when they have recently met. wolbachia pipientis, a common vertically transmitted symbiont of invertebrates, often has the capacity to sp ... | 2014 | 24594301 |
| three strains of wolbachia pipientis and high rates of infection in iranian sandfly species. | individual wild-caught sandflies from iran were examined for infections of wolbachia pipientis by targeting the major surface protein gene wsp of this intracellular α-proteobacterium. in total, 638 male and female sandflies were screened, of which 241 were found to be positive for one of three wsp haplotypes. regardless of geographical origins and habitats, phlebotomus (phlebotomus) papatasi and other sandfly species were found to be infected with one common, widespread strain of a-group w. pipi ... | 2014 | 24484966 |
| strength of the pathogenicity caused by feminizing wolbachia after transfer in a new host: strain or dose effect? | the alphaproteobacteria wolbachia pipientis are among the most common and widespread symbionts in the animal world. their vertical transmission mode is predicted to favour genotypes with low virulence. on the contrary, horizontal transfers of wolbachia from one host to another have been shown to possibly increase the symbiont virulence. this situation has been previously described when two feminizing wolbachia strains, wvulc and wvulm, from the ovaries of the woodlouse armadillidium vulgare were ... | 2014 | 24345405 |
| the impact of wolbachia, male age and mating history on cytoplasmic incompatibility and sperm transfer in drosophila simulans. | most insects harbour a variety of maternally inherited endosymbionts, the most widespread being wolbachia pipientis that commonly induce cytoplasmic incompatibility (ci) and reduced hatching success in crosses between infected males and uninfected females. high temperature and increasing male age are known to reduce the level of ci in a variety of insects. in drosophila simulans, infected males have been shown to mate at a higher rate than uninfected males. by examining the impact of mating rate ... | 2014 | 24164708 |
| monophyly of wolbachia pipientis genomes within drosophila melanogaster: geographic structuring, titre variation and host effects across five populations. | wolbachia pipientis is one of the most widely studied endosymbionts today, yet we know little about its short-term adaptation and evolution. here, using a set of 91 inbred drosophila melanogaster lines from five populations, we explore patterns of diversity and recent evolution in the wolbachia strain wmel. within the d. melanogaster lines, we identify six major mitochondrial clades and four wmel clades. concordant with past studies, the wolbachia haplotypes contain an overall low level of nucle ... | 2013 | 24118111 |