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one-appointment technic to form a crown base. 20154619603
agglutination of african primate and rodent erythrocytes by adenoviruses, reoviruses, and enteroviruses.african nonhuman primate and rodent erythrocytes were tested for agglutination by adenoviruses, reoviruses, and enteroviruses. squirrel erythrocytes were agglutinated by reovirus serotypes and adenovirus types 3, 11, 16, and 21. adenoviruses also agglutinated brazza monkey erythrocytes to the same titers as those obtained with either rhesus or grey monkey cells. prototype reovirus types 1 and 2 agglutinated grey monkey erythrocytes to much lower titers than either squirrel or human group o red c ...19724631106
effect of weak acids on amino acid transport by penicillium chrysogenum: evidence for a proton or charge gradient as the driving force.a variety of weak acids at and below their pk(a) are potent inhibitors of transport in penicillium chrysogenum. the effective compounds include sorbate, benzoate, and propionate (common antifungal agents), indoleacetate (a plant hormone), acetylsalicylate (aspirin), hexachlorophene, and a yellow pigment produced by the mycelia under nutrient-deficient conditions, as well as the classical uncouplers 2,4-dinitrophenol, p-nitrophenol, and azide. the results suggest that a proton gradient or charge ...19734632394
bursicon: its effect on tyrosine permeation into insect haemocytes. 20154640774
human skeletal muscle phosphorylase activities with exercise and training. 19724640795
[experimental studies for the fluorochroming of human spermatozoa with special reference to so-called vital fluorochroming]. 19724651092
a computer for the calculation of skeletal muscle vascular resistance. 19724652900
[evaluation of early and long term results of the surgical treatment of dupuytren's contracture in controversy problem--partial or total fascietomy]. 20154664976
[methodology in nutrition surveys among preschool children]. 20154666043
[long-term follow-up of reconstruction of the pulmonary artery using homograft]. 20154676526
[drug effects on peripheral circulation in postthrombotic syndrome]. 20154678612
the concept of general experience: age and strategies in guessing future events. 20154683201
[criterion for optimization of sampling size]. 20154683655
[occupational medicine problems of an aviation physician]. 20154701759
[occupational medicine problems of an aviation physician]. 20154701759
response of body weight to a low carbohydrate, high fat diet in normal and obese subjects. 20154703054
[serum glycoproteins in peritonitis]. 20154703286
[radiographic cassette with goniometer and its use on orthopedic tables in operations of femoral neck fractures]. 20154705124
[radiographic cassette with goniometer and its use on orthopedic tables in operations of femoral neck fractures]. 20154705124
photochemistry of diazonium salts. i. synthesis of 4-fluoroimidazoles, 4-fluorohistamine, and 4-fluorohistidine. 20164730663
study of albumin synthesis in relation to urea synthesis. 19734746768
u wave alternans and increased ventricular irritability. 20154749392
editorial: investigative dermatology. 20164758728
[primary trigeminal neuralgia--odontologic points of view]. 20164766396
regional viewpoints on population growth: perspectives of the genocide issue.genocide implies an effort by the white races to reduce the number of or eliminate the colored races. in africa the belief that family planning is a way of advocating genocide combines with the problem of lack of real experts in the field of family planning and of disproportionate allocation of funds to this area without regard to the goals of overall development. additionally, some methods of fertility control are recommended by foreign "experts" which may be culturally unacceptable. in spite o ...20164788253
an analysis of meningitis cases admitted to strathclyde hospital, motherwell 1949-71. 20164794861
[the teratogenic effect of busulfan on testis cells of the fetal rat]. 19734800446
alterations in cerebral protein-synthesizing systems during maturation. 19734802996
evolution of the medical director concept. 20164808782
determination of lead using charged particle activation analysis. 20154814232
properties and cellular localization of chitin synthetase in phycomyces blakesleeanus. 20164817973
blepharochalasis. 20154824166
theory of competitive binding of iron by different transferrins. 20164824920
low false-alarm rates in signal detection by marine mammals. 20154833081
actin in the alga, chara corallina. 20164835552
[spontaneous recovery from eosinophilic granuloma of bones of the cranial vault]. 20164849757
[primary and secondary plastic surgery after mandibular resection. (measures for restoration of normal facial structure and jaw function)]. 20164869337
occlusion and prosthetic dentistry. 20164892616
[on the pathogenesis of experimental calciphylaxis of salivary glands]. 19694895236
human growth hormone and hypopituitargrowth retardation. 19694898810
the distribution of tamm horsfall mucoprotein (uromucoid) in the human nephron. 19694906366
[studies on the direct bonding of the orthodontic resin bracket to the tooth enamel. 2. the effect of pretreatment on the enamel surface]. 19694906741
a classification of surgical and congenital defects of the head and neck. 20164916391
[on a proposed law making obligatory the declaration of viral hepatitis and concerning disinfection]. 20164928868
aging under glass. 20164929456
policy decisions facing the united states in financing and organizing health care. 20164958246
airway management in patients with pierre robin syndrome. 20164959074
long-term total parenteral nutrition with growth, development, and positive nitrogen balance. 19684968812
indications of an interspecific transformation in allomyces. 20164974871
experimental variola in monkeys. i. studies on disease enhancing property of cortisone in smallpox. a preliminary report. 19684979039
analog computer simulation of log(k)-ph profiles. 20154982391
[salmonella infections, with special reference to carrier state]. 19704988175
[a tentative experimental analysis of upright posture]. 19725028037
radical prostatectomy in the management of carcinoma of the prostate: probable causes of some therapeutic failures. 20165033963
[results after 5 years of trial therapy in cutaneous tuberous angiomas]. 20165035075
studies on muscle rigidity: droperidol, diazepam, and promethazine. 20165062116
tablet granulations composed of spherical-shaped particles. 20165068953
inappropriate secretion of antidiuretic hormone in infectious pulmonary disease. 20165074478
reproduction of an inbred strain of culex pipiens prevented by loss of wolbachia pipientis. 19836404990
a rickettsia-like microorganism similar to wolbachia pipientis and its occurrence in culex mosquitoes. 19836863975
characterization of the unlinked 16s rdna and 23s-5s rrna operon of wolbachia pipientis, a prokaryotic parasite of insect gonads.the rrna-encoding genes (rdnas) have been cloned and characterized from wolbachia pipientis (wp), the gonadial bacteria-like parasite of the mosquito culex pipiens (cp) and the moth ephestia cautella (ec). in wp from both insect species the rdnas are organized in a way which appears to be very unusual. the rrnas are encoded by two unlinked transcription units, each present in a single copy per genome. one contains the 16s rdna only, while the other is an operon encoding both the 23s and 5s rdnas ...19957489921
wolbachia pipientis: bacterial density and unidirectional cytoplasmic incompatibility between infected populations of aedes albopictus.unidirectional cytoplasmic incompatibility is seen when certain wolbachia-infected insect populations are crossed. two hypotheses might explain this phenomenon: superinfections with mutually incompatible strains of wolbachia producing incompatibility when crossed to individuals infected with only a single bacterial strain or, alternatively, a bacterial dosage model, with differences in wolbachia densities responsible for the incompatibility. a quantitative pcr assay was set up as a general metho ...19957498425
organization of wolbachia pipientis in the drosophila fertilized egg and embryo revealed by an anti-wolbachia monoclonal antibody.cytoplasmic incompatibility (ci) in drosophila is related to the presence of wolbachia, an intracellular microorganism found in many species of insects. in order to study the intracellular localization of wolbachia in eggs and embryos, we have purified the bacteria from fly embryos and subsequently generated a monoclonal antibody (mab wol-1) specific for wolbachia. indirect immunofluorescence staining using wol-1 reveals that during mitosis, wolbachia are localized near spindle poles and centros ...19957547474
increased male fertility in tribolium confusum beetles after infection with the intracellular parasite wolbachia.the cytoplasmically inherited microorganism wolbachia pipientis behaves like a sexually selected trait in its host, the flour beetle tribolium confusum, enhancing male fertility at the expense of female fecundity. here we show that infected females have fewer offspring than uninfected females but infected males have a large fertility advantage over uninfected males within multiply-inseminated infected or uninfected females. the male fertility effect accelerates the spread of the wolbachia throug ...19957800041
a prokaryotic dnaa sequence in drosophila melanogaster: wolbachia infection and cytoplasmic incompatibility among laboratory strains.using oligonucleotide primers derived from the aligned polypeptide sequences of several prokaryotic dnaa genes, we amplified from drosophila melanogaster dna a 557 bp fragment containing a single open reading frame. the predicted peptide sequence shows a significant similarity to previously characterized protein sequences that are encoded by the dnaa genes of several prokaryotes. the dnaa sequences are also detectable by pcr in dna from drosophila simulans and nasonia vitripennis flies which are ...19947894745
taxonomic position of the rickettsiae: current knowledge.the term rickettsiae initially encompassed all intracellular bacteria. early rickettsial taxonomy was based on a comparison of a few phenotypic characteristics and recently, molecular studies brought new bases for rickettsial taxonomy. all rickettsial species studied so far belong to the alpha and gamma groups of the proteobacteria. ehrlichiae complex groups cowdria ruminantium, anaplasma marginale and wolbachia pipientis and the related parthenogenesis and cytoplasmic incompatibility bacteria, ...19948117465
phylogenetic analysis of the genus rickettsia by 16s rdna sequencing.rickettsiae are gram-negative bacteria which multiply only inside host cells and need arthropods either as reservoirs or as vectors. using the polymerase chain reaction and an automated laser fluorescent dna sequencer, we amplified and sequenced the 16s rrna (rdna) of all available bacteria of the genus rickettsia. r. tsutsugamushi remained close to the other bacteria of the genus rickettsia using this technique, contrary to previous conclusions based on the study of the sta-58 protein antigen. ...19958525055
an improved microinjection protocol for the transfer of wolbachia pipientis between infected and uninfected strains of the flour beetle tribolium confusum.the lethality of halocarbon and other oils to hatching larvae of the flour beetle tribolium confusum limits existing microinjection protocols, because postinjection survivorship is only 5.6% of the eggs injected. we report the development of an oil-free protocol that improves survivorship fivefold. we used this protocol to transfect the cytoplasmic endosymbiont wolbachia pipientis from infected donor eggs to uninfected host eggs and observed reproductive incompatibility in 40% of the surviving, ...19968764685
in vitro cultivation of wolbachia pipientis in an aedes albopictus cell line.a continuous cell line, aa23, was established from eggs of a strain of the asian tiger mosquito, aedes albopictus, naturally infected with the intracellular symbiont wolbachia pipientis. the resulting cell line was shown to be persistently infected with the bacterial endosymbiont. treatment with antibiotics cured the cells of the infection. in the course of establishing this cell line it was noticed that rflps in the pcr products of two wolbachia genes from the parental mosquitoes were fixed in ...19979013253
phylogeny and potential transmission routes of midgut-associated endosymbionts of tsetse (diptera:glossinidae).many tsetse species (diptera: glossinidae) harbour two morphologically different intracellular endosymbiotic microorganisms associated with gut tissue: primary (p) and secondary (s) endosymbionts. the p-endosymbionts of tsetse (wigglesworthia glossinidia) are sequestered in specialized epithelial cells, bacteriocytes, which form a structure (bacteriome) in the anterior portion of the gut. phylogenetic characterization of p-endsymbionts from the three subgenera of genus glossina has shown that th ...19979099582
ultrastructural differentiation of the genogroups in the genus ehrlichia.ultrastructural characteristics of 15 strains and isolates of ehrlichiae belonging to three genogroups, or clades of genetically related organisms united in the genera ehrlichia, cowdria, anaplasma, neorickettsia and a strain of wolbachia pipientis which represents a fourth genogroup in this cluster of species, were studied in continuous cell culture or in vivo: e. canis (oklahoma strain and vhe isolate), e. muris (as 145), e. chaffeensis (arkansas, 91he17 and sapulpa), human granulocytic ehrlic ...19989511829
overcoming cytoplasmic incompatibility in drosophila.the endocellular microbe wolbachia pipientis infects a wide variety of invertebrate species, in which its presence is closely linked to a form of reproductive failure termed cytoplasmic incompatibility (ci). ci renders infected males unable to father offspring when mated to uninfected females. because ci can dramatically affect fitness in natural populations, mechanisms that abate ci can have equally large impacts on fitness. we have discovered that repeated copulation by wolbachia-infected male ...19989523438
phylogeny and pcr-based classification of wolbachia strains using wsp gene sequences.wolbachia are a group of intracellular inherited bacteria that infect a wide range of arthropods. they are associated with a number of different reproductive phenotypes in their hosts, such as cytoplasmic incompatibility, parthenogenesis and feminization. while it is known that the bacterial strains responsible for these different host phenotypes form a single clade within the alpha-proteobacteria, until now it has not been possible to resolve the evolutionary relationships between different wol ...19989569669
cloning and characterization of a gene encoding the major surface protein of the bacterial endosymbiont wolbachia pipientis.the maternally inherited intracellular symbiont wolbachia pipientis is well known for inducing a variety of reproductive abnormalities in the diverse arthropod hosts it infects. it has been implicated in causing cytoplasmic incompatibility, parthenogenesis, and the feminization of genetic males in different hosts. the molecular mechanisms by which this fastidious intracellular bacterium causes these reproductive and developmental abnormalities have not yet been determined. in this paper, we repo ...19989573188
evolution of wolbachia pipientis transmission dynamics in insects.wolbachia pipientis is an intracellular bacterial parasite of arthropods that enhances its transmission by manipulating host reproduction, most commonly by inducing cytoplasmic incompatibility. the discovery of isolates with modified cytoplasmic incompatibility phenotypes and others with novel virulence properties is an indication of the potential breadth of evolutionary strategies employed by wolbachia.199910390640
wolbachia pipientis: microbial manipulator of arthropod reproduction.the alpha-proteobacterium wolbachia pipientis is a very common cytoplasmic symbiont of insects, crustaceans, mites, and filarial nematodes. to enhance its transmission, w. pipientis has evolved a large scale of host manipulations: parthenogenesis induction, feminization, and male killing. w. pipientis's most common effect is a crossing incompatibility between infected males and uninfected females. little is known about the genetics and biochemistry of these symbionts because of their fastidious ...199910547686
phylogenetic analysis of the 16s rdna of the cytoplasmic bacterium wolbachia from the novel host folsomia candida (hexapoda, collembola) and its implications for wolbachial taxonomy.wolbachia pipientis are intracellular, transovarially inherited alpha-proteobacteria in invertebrates. four major wolbachia groups exist: a, b (contained in divergent arthropods), c and d (harbored by nematoda). by means of transmission electron microscopy, we observed wolbachia-like bacteria in a primitive insect, folsomia candida (hexapoda, collembola, isotomidae). 16s rdna analysis proved them to constitute a novel lineage, henceforth named group e, in the wolbachial phylogenetic tree. it sha ...199910556723
tissue distribution and prevalence of wolbachia infections in tsetse flies, glossina spp.tsetse flies glossina spp. (diptera: glossinidae) harbor three different symbiotic microorganisms, one being an intracellular rickettsia of the genus wolbachia. this bacterium infects a wide range of arthropods, where it causes a variety of reproductive abnormalities, one of which is termed cytoplasmic incompatibility (ci) that, when expressed, results in embryonic death due to disruptions in fertilization events. we report here that in colonized flies, wolbachia infections can be detected in 10 ...200010759311
offsetting effects of wolbachia infection and heat shock on sperm production in drosophila simulans: analyses of fecundity, fertility and accessory gland proteins.infection in drosophila simulans with the endocellular symbiont wolbachia pipientis results in egg lethality caused by failure to properly initiate diploid development (cytoplasmic incompatibility, ci). the relationship between wolbachia infection and reproductive factors influencing male fitness has not been well examined. here we compare infected and uninfected strains of d. simulans for (1) sperm production, (2) male fertility, and (3) the transfer and processing of two accessory gland protei ...200010790392
comparative genomics of mitochondrial dna in drosophila simulans.the current study compares the nucleotide variation among 22 complete mitochondrial genomes of the three distinct drosophila simulans haplotypes with intron 1 of the alcohol dehydrogenase-related locus. this is the first study to investigate the sequence variation of multiple complete mitochondrial genomes within distinct mitochondrial haplotypes of a single species. patterns of variation suggest distinct forces are influencing the evolution of mitochondrial dna (mtdna) and autosomal dna in d. s ...200010903373
expression of cytoplasmic incompatibility in drosophila simulans and its impact on infection frequencies and distribution of wolbachia pipientis.the aim of this study is to examine the expression of cytoplasmic incompatibility and investigate the distribution and population frequencies of wolbachia pipientis strains in drosophila simulans. nucleotide sequence data from 16s rdna and a wolbachia surface protein coding sequence and cytoplasmic incompatibility assays identify four distinct wolbachia strains: wha, wri, wma, and wau. the levels of cytoplasmic incompatibility between six lines carrying these strains of bacteria and three contro ...200011108593
occurrence of novel verrucomicrobial species, endosymbiotic and associated with parthenogenesis in xiphinema americanum-group species (nematoda, longidoridae).numerous micro-organisms have been described as cytoplasmic symbionts of eukaryotes. many so-called obligate endosymbionts rely exclusively on maternal (vertical or transovarial) transmission to maintain themselves, rendering them dependent on the host sex ratio, which they would tend to manipulate to their own advantage. the latter phenomenon is often associated with the presence of wolbachia pipientis (alpha-proteobacteria) in arthropods and nematodes. a potentially similar situation was disco ...200011155997
in vitro activity of antimicrobial agents against the endosymbiont wolbachia pipientis.arthropod-transmitted (filarial) nematodes are important causes of disease in humans in tropical countries, yet no safe drug appropriate for mass delivery kills the adult worms. however, most filarial nematodes contain rickettsia-like bacteria of the genus wolbachia, and related bacteria also occur in insects. there is increasing evidence that these bacteria have significant functions in the biology of filarial nematodes. they are thus important targets in the search for antifilarial drugs and e ...200111328780
a newly discovered bacterium associated with parthenogenesis and a change in host selection behavior in parasitoid wasps.the symbiotic bacterium wolbachia pipientis has been considered unique in its ability to cause multiple reproductive anomalies in its arthropod hosts. here we report that an undescribed bacterium is vertically transmitted and associated with thelytokous parthenogenetic reproduction in encarsia, a genus of parasitoid wasps. although wolbachia was found in only one of seven parthenogenetic encarsia populations examined, the "encarsia bacterium" (eb) was found in the other six. among seven sexually ...200111592990
wolbachia density and virulence attenuation after transfer into a novel host.the factors that control replication rate of the intracellular bacterium wolbachia pipientis in its insect hosts are unknown and difficult to explore, given the complex interaction of symbiont and host genotypes. using a strain of wolbachia that is known to over-replicate and shorten the lifespan of its drosophila melanogaster host, we have tracked the evolution of replication control in both somatic and reproductive tissues in a novel host/wolbachia association. after transinfection (the transf ...200211880639
electron microscopic and molecular identification of wolbachia endosymbionts from onchocerca lupi: implications for therapy.it was recently demonstrated that wolbachia intracellular bacteria (alpha 2 proteobacteria, rickettsiales) living in filarial nematodes are obligatory symbionts of their hosts. herein, we report the electron microscopic and 16s ribosomal dna-based (16s rdna) identification of the endobacteria harboring in onchocerca lupi. the worm nodules containing the nematodes were removed from three hungarian dogs naturally infected with o. lupi. wolbachia-like endobacteria were detected by electron microsco ...200211992713
a host parasite interaction rescues drosophila oogenesis defects.the cytoplasmically inherited bacterium wolbachia pipientis is a widespread parasite of arthropods that manipulates the reproductive biology of its hosts, often to their detriment, in order to foster its own transmission through egg cytoplasm. here we report that infection by wolbachia restores fertility to drosophila melanogaster mutant females prevented from making eggs by protein-coding lesions in sex-lethal (sxl), the master regulator of sex determination. suppression of sterility by wolbach ...200212097909
host-symbiont conflicts: positive selection on an outer membrane protein of parasitic but not mutualistic rickettsiaceae.the rickettsiaceae is a family of intracellular bacterial symbionts that includes both vertically transmitted parasites that spread by manipulating the reproduction of their host (wolbachia in arthropods) and horizontally transmitted parasites (represented by cowdria ruminantium), and mutualists (wolbachia pipientis in nematode worms). we have investigated the nature of natural selection acting on an outer membrane protein, the wsp gene in wolbachia and its homologue map1 in cowdria, thought lik ...200212140246
within- and between-population variation for wolbachia-induced reproductive incompatibility in a haplodiploid mite.wolbachia pipientis is a bacterium that induces cytoplasmic incompatibility (ci), the phenomenon in which infected males are reproductively incompatible with uninfected females. ci spreads in a population of hosts because it reduces the fitness of uninfected females relative to infected females. ci encompasses two steps: modification (mod) of sperm of infected males and rescuing (resc) of these chromosomes by wolbachia in the egg. infections associated with ci have mod+ resa+ phenotypes. however ...200212206235
wolbachia distribution and cytoplasmic incompatibility during sperm development: the cyst as the basic cellular unit of ci expression.the growth and distribution of the intracellular microbe wolbachia pipientis during spermatogenesis in several different host/symbiont genetic combinations in drosophila melanogaster and drosophila simulans is described. considerable intra- and inter-strain variation in wolbachia density and tissue distribution was observed. wolbachia were found inside spermatocytes and spermatids or within the somatic cyst cells surrounding the germ cells. some strains displayed both tissue distributions. high ...200312559491
differences in extent of genetic introgression between sympatric culex pipiens and culex quinquefasciatus (diptera: culicidae) in california and south africa.comparisons of five morphological characters, 12 enzyme electrophoresis profiles, and wolbachia pipientis infection rates were used to characterize populations of members of the culex pipiens l. complex in california and south africa. in south africa, male phallosome dv/d ratio, male maxillary palp index, branching of siphonal seta 1a, the enzyme locus mdhp-1, and w. pipientis infection rates proved highly diagnostic for separating culex quinquefasciatus from cx. pipiens phenotypes. in johannesb ...200312597651
molecular detection of bartonella quintana, b. koehlerae, b. henselae, b. clarridgeiae, rickettsia felis, and wolbachia pipientis in cat fleas, france.the prevalences of bartonella, rickettsia, and wolbachia were investigated in 309 cat fleas from france by polymerase chain reaction (pcr) assay and sequencing with primers derived from the glta gene for rickettsia, the its and pap31 genes for bartonella, and the 16s rrna gene for anaplasmataceae. positive pcr results were confirmed by using the lightcycler and specific primers for the rompb of rickettsia and glta of bartonella. r. felis was detected in 25 fleas (8.1%), w. pipientis, an insect s ...200312643829
wolbachia pipientis growth kinetics and susceptibilities to 13 antibiotics determined by immunofluorescence staining and real-time pcr.wolbachia spp. are strict intracellular bacteria that infect a wide range of arthropods and filarial nematodes. filarial nematodes are important causes of human diseases. there is increasing evidence that wolbachia spp. influence important functions in the biology of the hosts, specifically, infertility. preliminary experiments with humans and animals have suggested that antibiotics with activity against wolbachia may help to treat filariasis. in this study, we determined using a real-time quant ...200312709338
rna polymerase beta-subunit-based phylogeny of ehrlichia spp., anaplasma spp., neorickettsia spp. and wolbachia pipientis.sequence analysis of rpob, the gene encoding the beta-subunit of rna polymerase, was used in a phylogenetic investigation of nine species from the genera ehrlichia, neorickettsia, wolbachia and anaplasma. the complete nucleotide sequences obtained for anaplasma phagocytophilum (hge agent), ehrlichia chaffeensis, neorickettsia sennetsu, neorickettsia risticii, anaplasma marginale and wolbachia pipientis were amongst the longest rpob sequences in genbank and ranged from 4074 bp for n. sennetsu to ...200312710612
endosymbiotic bacterium wolbachia pipientis in synanthropic populations of the mosquito culex pipiens pipiens l. (diptera, culicidae). 200312854423
sperm chromatin remodelling and wolbachia-induced cytoplasmic incompatibility in drosophila.wolbachia pipientis is an obligate bacterial endosymbiont, which has successfully invaded approximately 20% of all insect species by manipulating their normal developmental patterns. wolbachia-induced phenotypes include parthenogenesis, male killing, and, most notably, cytoplasmic incompatibility. in the future these phenotypes might be useful in controlling or modifying insect populations but this will depend on our understanding of the basic molecular processes underlying insect fertilization ...200312897857
the distribution and evolutionary history of wolbachia infection in native and introduced populations of the invasive argentine ant (linepithema humile).wolbachia pipientis is a maternally transmitted bacterium that often alters the life history of its insect host to maximize transmission to subsequent generations. here we report on the frequency and distribution of wolbachia infection in a widespread invasive species, the argentine ant (linepithema humile). we screened 1175 individual argentine ants from 89 nests on five continents and several islands, including numerous locations in both the native (south american) and introduced ranges. we de ...200314629385
mitochondrial and wolbachia markers for the sandfly phlebotomus papatasi: little population differentiation between peridomestic sites and gerbil burrows in isfahan province, iran.in iran, phlebotomus papatasi (scopoli) (diptera: psychodidae) is the main vector of leishmania major yakimoff & schokhor (kinetoplastida: trypanosomatidae), the causative agent of rural zoonotic cutaneous leishmaniasis. this sandfly is abundant both in villages and in the burrows of the main reservoir host, the gerbil rhombomys opimus (licht.) (rodentia: gerbillidae). populations of p. papatasi were sampled from the edges of villages in isfahan province, using cdc miniature light traps in perid ...200314651648
culture and phenotypic characterization of a wolbachia pipientis isolate.the recent isolation of wolbachia pipientis in the continuous cell line aa23, established from eggs of a strain of the asian tiger mosquito aedes albopictus, allowed us to perform extensive characterization of the isolate. bacterial growth could be obtained in c6/36, another a. albopictus cell line, at 28 degrees c and in a human embryonic lung fibroblast monolayer at 28 and 37 degrees c, confirming that its host cell range is broader than was initially thought. the bacteria were best visualized ...200314662922
phylogenomics of the reproductive parasite wolbachia pipientis wmel: a streamlined genome overrun by mobile genetic elements.the complete sequence of the 1,267,782 bp genome of wolbachia pipientis wmel, an obligate intracellular bacteria of drosophila melanogaster, has been determined. wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different hosts, which range from many forms of reproductive parasitism to mutualistic symbioses. analysis of the wmel genome, in particular phylogenomic comparisons with other intracellular bacteria, has reveale ...200415024419
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