Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
hyponatraemia in patients with cirrhosis.hyponatraemia occurs in nearly half of patients in hospital with cirrhosis and ascites, and is due to the excessive retention of free water which results from the kidney's inability to excrete it normally. the morbidity and mortality associated with hyponatraemia is largely attributable to central nervous system disturbances. the degree to which brain water content increases depends on the duration of hyponatraemia and on compensatory mechanisms. altered steroid and peptide hormones in cirrhotic ...20082217672
standardization of a colorimetric method to quantify growth and metabolic activity of wolbachia-infected mosquito cells.the aedes albopictus aa23 cell line, which is persistently infected with wolbachia pipientis strain walbb, tends to grow as aggregated clusters of cells that are difficult to disperse for conventional quantification based on cell number. we used a. albopictus c7-10 cells to validate conversion of methylthiazole tetrazolium (mtt) to a colored formazan product with respect to incubation time, cell number over a 40-fold range, and metabolic activity as cells enter stationary phase. using this assay ...200818622662
effects of constraint on the oscillatory pattern of endosonic files.when operated in air, the transverse oscillation of the endosonic file exhibits a series of nodes and antinodes along its length with the greatest displacement amplitude occurring at the unconstrained tip. endosonic files of varying design, length, and thickness exhibit differences in their oscillation. preliminary work has revealed that the endosonic file is susceptible to constraint when working within the root canal and a model system was designed to investigate this problem. the constraining ...20082607288
differential expression of the il-2 receptor subunits, p55 and p75 on various populations of primary peripheral blood mononuclear cells.unstimulated pbl were examined for expression of il-2r subunits, il-2rp55 and il-2rp75, by two-color flow cytometric analyses using mab. nkh-1+ non-t non-b cells expressed il-2rp75 but not il-2rp55, and the il-2rp75 sites on purified nkh-1+ cells were determined to be 1630 sites/cell by binding of 125i-labeled tu27 mab specific for il-2rp75. in the cd4+ t cell population, il-2rp55+ cells were significantly detected, but little or marginally of the il-2rp75+ cells. however, il-2rp75+ cells were s ...20082584707
[clinical study of osteoporosis]. 20082614196
molecular and functional characterization of granulin-like molecules of insects.granulins are a group of highly conserved growth factors that have been described from a variety of organisms spanning the metazoa. here, we report on the identification of two partial transcripts encoding granulin-like molecules from aa23 embryonic cells of aedes albopictus and primary haemocytes from manduca sexta. both these partial transcripts had the characteristic 12-cysteine motif that is a hallmark of the granulin family and they represent the first granulin mrna transcripts identified f ...200818405836
genetic and cytogenetic analysis of the fruit fly rhagoletis cerasi (diptera: tephritidae).the european cherry fruit fly, rhagoletis cerasi, is a major agricultural pest for which biological, genetic, and cytogenetic information is limited. we report here a cytogenetic analysis of 4 natural greek populations of r. cerasi, all of them infected with the endosymbiotic bacterium wolbachia pipientis. the mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of this pest species are presented here. the mitotic metaphase complement consists of 6 pairs of ...200818545272
genome evolution of wolbachia strain wpip from the culex pipiens group.the obligate intracellular bacterium wolbachia pipientis strain wpip induces cytoplasmic incompatibility (ci), patterns of crossing sterility, in the culex pipiens group of mosquitoes. the complete sequence is presented of the 1.48-mbp genome of wpip which encodes 1386 coding sequences (cdss), representing the first genome sequence of a b-supergroup wolbachia. comparisons were made with the smaller genomes of wolbachia strains wmel of drosophila melanogaster, an a-supergroup wolbachia that is al ...200818550617
cloning, expression, purification and characterization of a dsba-like protein from wolbachia pipientis.wolbachia pipientis are obligate endosymbionts that infect a wide range of insect and other arthropod species. they act as reproductive parasites by manipulating the host reproduction machinery to enhance their own transmission. this unusual phenotype is thought to be a consequence of the actions of secreted wolbachia proteins that are likely to contain disulfide bonds to stabilize the protein structure. in bacteria, the introduction or isomerization of disulfide bonds in proteins is catalyzed b ...200818387819
simple diagnosis of the zellweger syndrome by gas-liquid chromatography of dimethylacetals.the absence of peroxisomes in patients with the cerebrohepatorenal syndrome of zellweger leads to several biochemical abnormalities, including deficient synthesis of plasmalogens as well as accumulation of very long-chain fatty acids and intermediates in bile acid biosynthesis. accumulation of very long-chain fatty acids in serum and fibroblasts has hitherto been used most extensively for diagnosis. due to the relatively small amounts of the very long-chain fatty acids also in the zellweger pati ...20083760714
[ki-1 large cell lymphoma with secondary xanthomatous infiltration of the lesions by chemotherapy]. 20082575882
the untreated, shrinking simple bone cavity: case report. 20083064707
iswpi1 from wolbachia pipientis defines a novel group of insertion sequences within the is5 family.insertion sequences are transposable elements that can represent substantial proportions of prokaryotic genomes and play a substantial role in shaping host genome evolution. as such, evaluating and understanding insertion sequence diversity is an important task to fulfill, because it is expected to yield new insight into the evolution of bacterial transposable elements and contribute to improve genome annotations. here, i characterized an insertion sequence, termed iswpi1, for which the taxonomi ...200818155858
host adaptation of a wolbachia strain after long-term serial passage in mosquito cell lines.the horizontal transfer of the bacterium wolbachia pipientis between invertebrate hosts hinges on the ability of wolbachia to adapt to new intracellular environments. the experimental transfer of wolbachia between distantly related host species often results in the loss of infection, presumably due to an inability of wolbachia to adapt quickly to the new host. to examine the process of adaptation to a novel host, we transferred a life-shortening wolbachia strain, wmelpop, from the fruit fly dros ...200818836024
wolbachia and virus protection in insects.wolbachia pipientis bacteria are common endosymbionts of insects that are best known for their ability to increase their prevalence in populations by manipulating host reproductive systems. however, there are examples of wolbachia that exist in nature that seem to induce no reproductive parasitism trait and yet are able to invade populations. we demonstrate a fitness benefit for wolbachia-infected insects that may explain this paradox. drosophila melanogaster flies infected with wolbachia are le ...200818974344
diversity of ixodes ricinus tick-associated bacterial communities from different forests.nymphal ixodes ricinus ticks (n=180) were collected from three different areas in the netherlands to investigate the effect of forest composition on tick-associated microbial communities. sampled habitats differed in thickness of leaf litter and humus layers and vegetation associations and were located near amsterdam (beech-oak), ede (birch-oak) and veldhoven (birch-oak). analysis of nine 16s rrna gene clone libraries made from individual ticks showed nearest matches with presumed pathogens cand ...200818355299
crystallization and preliminary diffraction analysis of a dsba homologue from wolbachia pipientis.alpha-dsba1 is one of two dsba homologues encoded by the gram-negative alpha-proteobacterium wolbachia pipientis, an endosymbiont that can behave as a reproductive parasite in insects and as a mutualist in medically important filarial nematodes. the alpha-dsba1 protein is thought to be important for the folding and secretion of wolbachia proteins involved in the induction of reproductive distortions. crystals of native and semet alpha-dsba1 were grown by vapour diffusion and belong to the monocl ...200818259058
wolbachia infection alters olfactory-cued locomotion in drosophila spp.wolbachia pipientis is an endosymbiotic bacterium present in diverse insect species. although it is well studied for its dramatic effects on host reproductive biology, little is known about its effects on other aspects of host biology, despite its presence in a wide array of host tissues. this study examined the effects of three wolbachia strains on two different drosophila species, using a laboratory performance assay for insect locomotion in response to olfactory cues. the results demonstrate ...200818456851
the endosymbiont wolbachia pipientis induces the expression of host antioxidant proteins in an aedes albopictus cell line.wolbachia are obligate intracellular bacteria which commonly infect arthropods. they are maternally inherited and capable of altering host development, sex determination, and reproduction. reproductive manipulations include feminization, male-killing, parthenogenesis, and cytoplasmic incompatibility. the mechanism by which wolbachia avoid destruction by the host immune response is unknown. generation of antimicrobial peptides (amps) and reactive oxygen species (ros) by the host are among the fir ...200818461124
genetic and functional characterization of the type iv secretion system in wolbachia.a type iv secretion system (t4ss) is used by many symbiotic and pathogenic intracellular bacteria for the successful infection of and survival, proliferation, and persistence within hosts. in this study, the presence and function of the t4ss in wolbachia strains were investigated by a combination of genetic screening and immunofluorescence microscopy. two operons of virb-vird4 loci were found in the genome of wolbachia pipientis strain watab3, from the hymenoptera asobara tabida, and strain wri, ...200818502862
cytological properties of an aedes albopictus mosquito cell line infected with wolbachia strain walbb.in vitro production of the obligate intracellular bacterium, wolbachia pipientis, is essential to its manipulation as a genetic tool to spread transgenes within vector populations. we have adapted the wolbachia-infected aa23 aedes albopictus mosquito cell line to eagle's minimal medium, supplemented with nonessential amino acids, glutamine, and 20% fetal bovine serum. when plated at low densities, aa23e cells grew as patchy monolayers, comprised of non-contiguous clusters of cells that gave rise ...200818401667
the endosymbiont wolbachia increases insulin/igf-like signalling in drosophila.insulin/igf-like signalling (iis) is an evolutionarily conserved pathway that has diverse functions in multi-cellular organisms. mutations that reduce iis can have pleiotropic effects on growth, development, metabolic homeostasis, fecundity, stress resistance and lifespan. iis is also modified by extrinsic factors. for instance, in the fruitfly drosophila melanogaster, both nutrition and stress can alter the activity of the pathway. here, we test experimentally the hypothesis that a widespread e ...200919692410
wolbachia infection reduces blood-feeding success in the dengue fever mosquito, aedes aegypti.the mosquito aedes aegypti was recently transinfected with a life-shortening strain of the endosymbiont wolbachia pipientis (wmelpop) as the first step in developing a biocontrol strategy for dengue virus transmission. in addition to life-shortening, the wmelpop-infected mosquitoes also exhibit increased daytime activity and metabolic rates. here we sought to quantify the blood-feeding behaviour of wolbachia-infected females as an indicator of any virulence or energetic drain associated with wol ...200919753103
proteasome activity in a naïve mosquito cell line infected with wolbachia pipientis walbb.we used wolbachia pipientis strain walbb from aedes albopictus aa23 cells to infect clonal ae. albopictus tk-6 cells, which are resistant to 5-bromodeoxyuridine. infected tk-6 cells were cultured in medium containing 5-bromodeoxyuridine to select against aa23 cells that might have persisted in the inoculum. infected tk-6 lines retained the wolbachia infection for 5 mo, indicating that their metabolic processes support wolbachia growth and multiplication. to investigate early events after wolbach ...200919296184
extensive genomic diversity of closely related wolbachia strains.using microarray-based comparative genome hybridization (mcgh), the genomic content of wolbachia pipientis wmel from drosophila melanogaster was compared to the closely related wolbachia from d. innubila (winn), d. santomea (wsan), and three strains from d. simulans (wau, wri, wsim). a large number of auxiliary genes are identified in these five strains, with most absent/divergent genes being unique to a given strain. each strain caused an average of approximately 60 genes to be removed from the ...200919389774
increased locomotor activity and metabolism of aedes aegypti infected with a life-shortening strain of wolbachia pipientis.a virulent strain of the obligate intracellular bacterium wolbachia pipientis that shortens insect lifespan has recently been transinfected into the primary mosquito vector of dengue virus, aedes aegypti l. the microbe's ability to shorten lifespan and spread through host populations under the action of cytoplasmic incompatibility means it has the potential to be used as a biocontrol agent to reduce dengue virus transmission. wolbachia is present in many host tissues and may have local effects o ...200919411536
wolbachia infection and resource competition effects on immature aedes albopictus (diptera: culicidae).wolbachia pipientis hertig and wolbach (rickettsiales: rickettsiaceae) are intracellular alpha-proteobacteria that occur naturally in aedes albopictus (skuse) (diptera: culicidae) and numerous other invertebrates. these endosymbionts can invade host populations by manipulating host reproduction. wolbachia infections have been shown to impart both costs and benefits to hosts in terms of development, survival, and fecundity. here, we monitor intraspecific competition among independent cohorts of i ...200919496412
wolbachia induces strong cytoplasmic incompatibility in the predatory bug macrolophus pygmaeus.macrolophus pygmaeus is a heteropteran predator that is widely used in european glasshouses for the biological control of whiteflies, aphids, thrips and spider mites. we have demonstrated that the insect is infected with the endosymbiotic bacterium wolbachia pipientis. several gene fragments of the endosymbiont were sequenced and subsequently used for phylogenetic analysis, revealing that it belongs to the wolbachia supergroup b. the endosymbiont was visualized within the ovarioles using immunol ...200919523069
the virulent wolbachia strain wmelpop efficiently establishes somatic infections in the malaria vector anopheles gambiae.wolbachia pipientis bacteria are maternally inherited endosymbionts that are of interest to control the anopheles mosquito vectors of malaria. wolbachia does not infect anopheles mosquitoes in nature, although cultured anopheles cells can be infected. here, we show that the virulent wolbachia strain wmelpop can survive and replicate when injected into female anopheles gambiae adults, but the somatic infections established are avirulent. these in vivo data suggest that stable wolbachia infections ...200919329661
directly repeated sequences associated with pathogenic mitochondrial dna deletions.we determined the nucleotide sequences of junctional regions associated with large deletions of mitochondrial dna found in four unrelated individuals with a phenotype of chronic progressive external ophthalmoplegia. in each patient, the deletion breakpoint occurred within a directly repeated sequence of 13-18 base pairs, present in different regions of the normal mitochondrial genome-separated by 4.5-7.7 kilobases. in two patients, the deletions were identical. when all four repeated sequences a ...20092813377
directly repeated sequences associated with pathogenic mitochondrial dna deletions.we determined the nucleotide sequences of junctional regions associated with large deletions of mitochondrial dna found in four unrelated individuals with a phenotype of chronic progressive external ophthalmoplegia. in each patient, the deletion breakpoint occurred within a directly repeated sequence of 13-18 base pairs, present in different regions of the normal mitochondrial genome-separated by 4.5-7.7 kilobases. in two patients, the deletions were identical. when all four repeated sequences a ...20092813377
history of medicine in alaska. harriet crocker jackson schirmer, m.d. 20092662801
[early functional treatment following sports injuries]. 20092718517
[familial infantile nephrotic syndrome with ocular abnormalities].two siblings born from consanguineous parents experienced infantile nephrotic syndrome with ocular and neurological abnormalities and a micropenis in the boy; both patients died before age 1. the opportunity to perform successively a renal biopsy and a two-step binephrectomy permitted a good histological follow-up. the lesions were characterized by mesangial involvement, followed by an extensive extracapillary proliferation and tubular dilatations with a high mitotic activity of the epithelium w ...20092812971
[familial infantile nephrotic syndrome with ocular abnormalities].two siblings born from consanguineous parents experienced infantile nephrotic syndrome with ocular and neurological abnormalities and a micropenis in the boy; both patients died before age 1. the opportunity to perform successively a renal biopsy and a two-step binephrectomy permitted a good histological follow-up. the lesions were characterized by mesangial involvement, followed by an extensive extracapillary proliferation and tubular dilatations with a high mitotic activity of the epithelium w ...20092812971
septic shock: principles of management in the emergency department.bacteremia is a potentially serious event which must be recognized early and treated aggressively to prevent progression to septicemia and septic shock. the pathophysiology of septicemia and shock includes inadequate tissue perfusion and oxygenation. expansion of intravascular volume and pharmacologic cardiovascular support are designed to minimize resulting end-organ injury. initial antibiotic therapy must be individualized and should include an agent or agents active against the common pathoge ...20092691993
response to "an interpretive study describing the clinical judgment of nurse practitioners". 20092772453
screening for cancer of the cervix. 20092687425
effects on male fitness of removing wolbachia infections from the mosquito aedes albopictus.cytoplasmic incompatibility (ci) induced by maternally inherited wolbachia bacteria is a potential tool for the suppression of insect pest species with appropriate patterns of infection. the asian tiger mosquito aedes albopictus (skuse) (diptera: culicidae) is known to be infected by two strains of wolbachia pipientis hertig (rickettsiales: rickettsiaceae), walb a and walb b, throughout its geographical distribution. this infection pattern theoretically restricts the application of ci-based cont ...200919292821
the mosaic genome structure of the wolbachia wri strain infecting drosophila simulans.the obligate intracellular bacterium wolbachia pipientis infects around 20% of all insect species. it is maternally inherited and induces reproductive alterations of insect populations by male killing, feminization, parthenogenesis, or cytoplasmic incompatibility. here, we present the 1,445,873-bp genome of w. pipientis strain wri that induces very strong cytoplasmic incompatibility in its natural host drosophila simulans. a comparison with the previously sequenced genome of w. pipientis strain ...200919307581
conservation of the type iv secretion system throughout wolbachia evolution.the type iv secretion system (t4ss) is an efficient pathway with which bacteria can mediate the transfer of dna and/or proteins to eukaryotic cells. in wolbachia pipientis, a maternally inherited obligate endosymbiont of arthropods and nematodes, two operons of vir genes, virb3-b6 and virb8-d4, encoding a t4ss were previously identified and characterized at two separate genomic loci. using the largest data set of wolbachia strains studied so far, we show that vir gene sequence and organization a ...200919486895
sce induction by ccnu and pregnancy. 20092704378
pcr analysis for wolbachia in human and canine demodex mites.in many skin diseases such as demodex folliculitis, rosacea- or steroid-induced rosacea demodex mites are present in abundance and are at least partially held responsible for causing these disorders. although it is known that these diseases respond well to tetracyclines, it is unclear if this is due to the antiinflammatory effects of the antibiotics or to an antibacterial effect on so far unknown bacteria within the demodex mites. as in filariasis, where the response to doxycycline can be explai ...200919652990
structural and functional characterization of the oxidoreductase alpha-dsba1 from wolbachia pipientis.the alpha-proteobacterium wolbachia pipientis is a highly successful intracellular endosymbiont of invertebrates that manipulates its host's reproductive biology to facilitate its own maternal transmission. the fastidious nature of wolbachia and the lack of genetic transformation have hampered analysis of the molecular basis of these manipulations. structure determination of key wolbachia proteins will enable the development of inhibitors for chemical genetics studies. wolbachia encodes a homolo ...200919265485
evidence for metabolic provisioning by a common invertebrate endosymbiont, wolbachia pipientis, during periods of nutritional stress.wolbachia are ubiquitous inherited endosymbionts of invertebrates that invade host populations by modifying host reproductive systems. however, some strains lack the ability to impose reproductive modification and yet are still capable of successfully invading host populations. to explain this paradox, theory predicts that such strains should provide a fitness benefit, but to date none has been detected. recently completed genome sequences of different wolbachia strains show that these bacteria ...200919343208
unravelling the wolbachia evolutionary role: the reprogramming of the host genomic imprinting.environmental factors can induce significant epigenetic changes that may also be inherited by future generations. the maternally inherited symbiont of arthropods wolbachia pipientis is an excellent candidate as an 'environmental' factor promoting trans-generational epigenetic changes: by establishing intimate relationships with germ-line cells, epigenetic effects of wolbachia symbiosis would be manifested as a 'maternal effect', in which infection of the mother modulates the offspring phenotype. ...200919364731
an ancient horizontal gene transfer between mosquito and the endosymbiotic bacterium wolbachia pipientis.the extent and biological relevance of horizontal gene transfer (hgt) in eukaryotic evolution remain highly controversial. recent studies have demonstrated frequent and large-scale hgt from endosymbiotic bacteria to their hosts, but the great majority of these transferred genes rapidly become nonfunctional in the recipient genome. here, we investigate an ancient hgt between a host metazoan and an endosymbiotic bacterium, wolbachia pipientis. the transferred gene has so far been found only in mos ...200918988686
[a case of immediate fertility restoration following childbirth].we report an unwanted pregnancy following immediately after fulltime childbirth. we could not find previous reports of this nature in international literature. women who give bottle-feeding are even more liable to early return of ovulatory cycles than women who give breastfeeding. if a puerpera has vaginal bleeding (not lochia), there is an increased risk of ovulation as well as of endometritis. it is no longer acceptable to take impossibility to conceive immediately after delivery for granted: ...20092654662
craniofacial manifestations of ehlers-danlos syndromes, cutis laxa syndromes, and cutis laxa-like syndromes. 20092697382
variation in antiviral protection mediated by different wolbachia strains in drosophila simulans.drosophila c virus (dcv) is a natural pathogen of drosophila and a useful model for studying antiviral defences. the drosophila host is also commonly infected with the widespread endosymbiotic bacteria wolbachia pipientis. when dcv coinfects wolbachia-infected d. melanogaster, virus particles accumulate more slowly and virus induced mortality is substantially delayed. considering that wolbachia is estimated to infect up to two-thirds of all insect species, the observed protective effects of wolb ...200919911047
[ultrastructural study of left ventricular myocardium in mitral regurgitation--correlations with postoperative cardiac functions].light and electron microscopic examination was carried out in tissue samples which were obtained from the left ventricular free wall in 21 patients with mitral regurgitation during open-heart surgery. their correlations with postoperative cardiac functions were also studied. the area of the interstitial fibrosis (%fib) was calculated from the light microscopic examination of the tissue samples, and changes of intracellular structures were expressed in scores by means of electron microscopy (em-s ...20092768908
inhibitory effect of disulfiram (antabuse) on alcohol dehydrogenase activity.we investigated the effect of disulfiram (antabuse) on the activity of alcohol dehydrogenase (ec 1.1.1.1) in vitro. we observed a time-dependent inhibition of this dehydrogenase by disulfiram and diethyldithiocarbamate similar to that obtained for aldehyde dehydrogenase (ec 1.2.1.3). these results suggest a possible explanation for various side effects observed in the clinical use of antabuse.20092822291
inhibitory effect of disulfiram (antabuse) on alcohol dehydrogenase activity.we investigated the effect of disulfiram (antabuse) on the activity of alcohol dehydrogenase (ec 1.1.1.1) in vitro. we observed a time-dependent inhibition of this dehydrogenase by disulfiram and diethyldithiocarbamate similar to that obtained for aldehyde dehydrogenase (ec 1.2.1.3). these results suggest a possible explanation for various side effects observed in the clinical use of antabuse.20092822291
a more cost-effective method of preoperative computerized imaging.stimulated by the explosive expansion of the computerized desk top publishing industry during the past few years, microcomputer hardware and software are evolving at a staggering rate. memory is rapidly increasing, and prices are declining. i have found that with the hardware and software described in this paper, i was able to obtain, in a much more cost-effective manner, as useful preoperative information for my practice as i could obtain with more expensive "turnkey" (only one use) computerize ...20092734393
[development of active research and seminar activities--at the nursing research training center of chiba university school of nursing]. 20092739161
[heparin therapy of pregnant women with placental insufficiency].in the presence of low-dosage administrations of heparin the system of hemostasis was studied in 50 pregnant females with placental failure and the chronic dic syndrome. the efficacy of the treatment conducted was assessed with regard to the revealed levels of coagulation natural inhibitors, the products of fibrinogen-fibrin degradation, the degree of thrombocytic functional recovery and stabilization of the chronometric values of coagulation. the latter parameters considered together with the l ...20092817276
model to evaluate ventricular insufficiency utilizing first-pass radioventriculogram component analysis. 20092738673
should we stop using theophylline for the treatment of the hospitalized patient with status asthmaticus?recent reviews and editorials have indicated that theophylline may not be effective for the therapy of acute severe asthma. this judgment is primarily based on data comparing the efficacy of theophylline to inhaled beta 2-agonists in the emergency room treatment of asthma. the authors believe that it is inappropriate to extrapolate data from short-term studies in the emergency room setting to the hospitalized patients with status asthmaticus. studies of hospitalized patients in which the patient ...20092690474
the potential use of cyclodextrins in parenteral formulations.the general use of cyclodextrins in drug formulations is reviewed. the ability of cyclodextrins to form reversible inclusion complexes with many drugs can eliminate various undesirable physicochemical properties. while beta-cyclodextrin is extremely useful in many of these applications, it is toxic when given parenterally, precluding its use in i.v. and other formulations. chemically modified cyclodextrins such as 2-hydroxypropyl-beta-cyclodextrin are amorphous isomeric mixtures which are potent ...20092681643
[intensive research on antiviral agents--a review]. 20092664385
mechanism of mammalian ovulation.the sequence of ovarian events during the process of ovulation discussed in this review is schematically represented in figure 1. it is obvious that lh, perhaps with some contribution from fsh, is the normal physiological trigger for the ovulatory sequence of events and it appears from the available information that lh's effects are mainly mediated via adenylate cyclase and increased camp. the camp in turn, via camp-dependent protein kinase, influences at least three distinct steps in the ovulat ...20092657783
quantitative pathologic analysis of first and second primary cancers in double tumors of the lung.in spite of the frequent occurrence of double tumors of the lung, pathologic reports on these tumors are rare. in this study, 34 patients with double tumors (10 metachronous and 24 synchronous) were quantitatively analyzed; in all cases, both the first and second tumors had been completely resected and had adequate archival material. one aim of the study was to investigate whether there was a difference in the malignancy of the first and second tumors, as evaluated from their pathologic features ...20092719795
modulation of postprandial glycaemia and insulinaemia by cellulose in mixed nutrient combinations.the present study was designed to examine the effect of cellulose (cl) on postprandial glycaemia and insulinaemia when ingested with glucose (g), casein (cs) and maize oil (co) in various combinations. the study was conducted on five healthy male volunteers, on each of whom five meal tolerance tests were performed. the meals were isoenergetic and consisted of g; g and cl; g, cs and cl; g, co and cl; g, cs, co and cl. the meals were administered after an overnight fast. in addition to a fasting v ...20092675962
the use of psychotropic agents in pregnancy and lactation.the question of which psychotropic medications are safe during pregnancy is likely to remain unanswered for many years to come. there are ethical limitations to performing the type of prospective controlled studies required to answer a scientific question of this type definitively. at the present time, in all patients with worsening psychiatric illness during pregnancy, be it in the schizophrenic, affective, anxiety disorder, or personality disorder spectrum, outpatient psychotherapy, hospitaliz ...20092652114
inter-urban comparison of ambient volatile organic compound concentration in u.s. cities. 20092732765
changing the system. 20092635967
clinical and immune recovery from omenn syndrome after bone marrow transplantation. 20092647948
application of nirs to the quantity control of pharmaceuticals ketoprofen assay in different pharmaceutical formulae.the possibility of using reflectance nirs to assay 2-(benzoylphenyl)-propionic acid (ketoprofen) in solid-state and gel preparations is investigated. the number of samples and amplitude of the percentage content distribution of the samples is examined in order to define the best possible calibration system.20092755959
severe hypotension from epidural meperidine in a high-risk patient after thoracotomy.a sixty-eight-year-old female developed severe hypotension immediately after the administration of epidural meperidine for post-thoracotomy pain. two preceding injections of epidural opiates had been uneventful. the cardiovascular collapse was difficult to reverse and may have contributed to the patient's subsequent death. cardiovascular complications have not been reported frequently as a possible side effect of epidural opiate analgesia.20092758544
[treatment of persistent cardiac failure by ultrafiltration].ultrafiltration, a method of extra renal filtering, which accomplishes an iso-osmotic removal of water an sodium from the plasma, was used in twelve patients. it is reserved for terminal persistent heart failures. in one case, it preceded a heart transplant. simple to carry out, this technique is well tolerated when the pulmonary pressure is elevated, respecting an ultrafiltration output inferior or equal to 500 ml/h. immediate improvement of the patients is spectacular. a new treatment failure ...20092735736
autoimmunity after induction of neonatal tolerance to alloantigens: role of b cell chimerism and f1 donor b cell activation.balb/c mice rendered tolerant by the neonatal injection of semiallogeneic (c57bl/6 x balb/c)f1 spleen cells develop features of autoimmune disease. the possible mechanisms involved in autoantibody production, particularly anti-dna antibodies, were investigated. in the first 5 wk, there was polyclonal b cell activation, as indicated by marked hypergammaglobulinemia, with a predominance of igg1 and an increased production of antihapten antibodies. igg1 anti-ssdna and anti-dsdna antibodies were det ...20102940295
4-amino-3-(2,3,5-tri-o-benzyl-beta-d-ribofuranosyl)-5-pyrazole-carb oni trile. synthesis and conversion into a sugar-blocked 5,7-disubstituted formycin analog.the title compound, a potential intermediate to protected c-nucleoside analogs related to formycin a, was synthesized via a new route wherein 2,3,5-tri-o-benzyl-1-o-p-nitrophenyl)-d-ribofuranose was converted to 2,5-anhydro-3,4,6-tri-o-benzyl-d-allonic acid, and further transformed into 4-(tert-butyloxycarbonyl)-5-ethoxycarbonyl-3-(2,3,5-tri-o-benzyl-beta-d- ribofuranosyl)pyrazole. after amidation and dehydration to form the 4-(tert-butyloxycarbonyl)-5-pyrazolecarbonitrile, acidolysis followed b ...20103401884
facial nerve hemangioma. 20102916829
pancreatic abnormalities in type 2 diabetes mellitus. 20102893166
[a comparative study of clinics: terminal care personnel need communication, support and comfort to provide good care]. 20102918796
changes in serum enzymes in moderate drinkers after an alcohol challenge.when 14 "moderate" drinkers abstained from alcohol for four weeks, the activity of gamma-glutamyltransferase (ggt; ec 2.3.2.2) in their serum showed a large decrease. immediately after the period of abstention, an orally given ethanol challenge of 1 g/kg produced a marked increase in serum ggt at 24 h, followed by a slow decline thereafter. aspartate amino-transferase activity in serum was significantly increased at 24 h; however, alkaline phosphate, alanine aminotransferase, and lactate dehydro ...20102894905
methimazole antagonises hla-dr3 suppression of aids. 20102880104
association of wolbachia with heartworm disease in cats and dogs.although the presence of adult dirofilaria immitis in the pulmonary arteries and its associated arteritis and thromboembolic disease can explain some of the manifestations of canine and feline heartworm disease, the cause of other findings remains unclear. cats with d. immitis antibodies but lacking adult parasites in the pulmonary arteries frequently develop histological lesions of the airways, resulting in a condition termed heartworm-associated respiratory disease. all d. immitis parasites ha ...201020193981
cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram.sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ...20103116451
[establishment of a new continuous cell line of drosophila melanogaster strain infected by the intracellular endosymbiotic bacterium wolbachia pipientis under natural conditions].wolbachia pipientis is an obligately intracellular bacterium infecting a number of arthropod and nematode species. at the body level, wolbachia infection may cause parthenogenesis, feminization of genetic males, male killing, or cytoplasmic incompatibility; it may also be asymptomatic. of special interest is dna transfer from wolbachia to the host insect genome, which was discovered recently. at the cellular level, the effects caused by wolbachia have been studied more poorly. only one of the kn ...201020198874
lutzomyia sand fly diversity and rates of infection by wolbachia and an exotic leishmania species on barro colorado island, panama.sand flies (diptera, psychodidae, phlebotominae) in the genus lutzomyia are the predominant vectors of the protozoan disease leishmaniasis in the new world. within the watershed of the panama canal, the cutaneous form of leishmaniasis is a continuous health threat for residents, tourists and members of an international research community. here we report the results of screening a tropical forest assemblage of sand fly species for infection by both leishmania and a microbe that can potentially se ...201020231892
characterization of a new aedes albopictus (diptera: culicidae)-wolbachia pipientis (rickettsiales: rickettsiaceae) symbiotic association generated by artificial transfer of the wpip strain from culex pipiens (diptera: culicidae).wolbachia is a maternally inherited endosymbiont inducing various effects in insects and other invertebrate hosts that facilitate the invasion of naive host populations. one of the effects is a form of sterility known as cytoplasmic incompatibility (ci) through which females are effectively sterilized when they mate with males harboring a different wolbachia strain. the repeated mass release of cytoplasmically incompatible males can be a tool to suppress insect populations. here, we attempt to i ...201020380298
decoupling of host-symbiont-phage coadaptations following transfer between insect species.transferring endosymbiotic bacteria between different host species can perturb the coordinated regulation of the host and bacterial genomes. here we use the most common maternally transmitted bacteria, wolbachia pipientis, to test the consequences of host genetic background on infection densities and the processes underlying those changes in the parasitoid wasp genus nasonia. introgressing the genome of nasonia giraulti into the infected cytoplasm of n. vitripennis causes a two-order-of-magnitud ...201020944019
sex and stripping: the key to the intimate relationship between wolbachia and host?wolbachia pipientis is known to infect only arthropods and nematodes (mainly filarial worms). a unique feature shared by the two phyla is the ability to replace the exoskeleton, a process known as ecdysis. this shared characteristic is thought to reflect a common ancestry. arthropod moulting is induced by the steroid hormone 20-hydroxyecdysone (20e) and a role for ecdysteroids in nematode ecdysis has also been suggested. removing wolbachia from filarial worms impairs the host's development. from ...201020585501
mito-nuclear genetic comparison in a wolbachia infected weevil: insights on reproductive mode, infection age and evolutionary forces shaping genetic variation.maternally inherited endosymbionts like wolbachia pipientis are in linkage disequilibrium with the mtdna of their hosts. therefore, they can induce selective sweeps, decreasing genetic diversity over many generations. this sex ratio distorter, that is involved in the origin of parthenogenesis and other reproductive alterations, infects the parthenogenetic weevil naupactus cervinus, a serious pest of ornamental and fruit plants.201021050430
lateral phage transfer in obligate intracellular bacteria (wolbachia): verification from natural populations.lateral transfer of mobile dna is a hallmark of bacteria with a free-living replicative stage; however, its significance in obligate intracellular bacteria and other heritable endosymbionts remains controversial. comparative sequence analyses from laboratory stocks infected with wolbachia pipientis provide some of the most compelling evidence that bacteriophage wo-b transfers laterally between infections of the same insect host. lateral transfer between coinfections, however, has been evaluated ...201019906794
infection with the wmel and wmelpop strains of wolbachia leads to higher levels of melanization in the hemolymph of drosophila melanogaster, drosophila simulans and aedes aegypti.introduction of the life-shortening strain of wolbachia pipientis, wmelpop, into the key dengue vector, aedes aegypti, and the anti-pathogen effects in wolbachia-infected hosts highlights the need for more research into its interactions with its original host, drosophila melanogaster, and the novel mosquito host. the visual difference in darkness between the eggs of wmelpop wolbachia-infected and uninfected mosquito hosts after egg deposition led to further investigation into melanization levels ...201021075139
wolbachia infection and mitochondrial diversity in the canine heartworm (dirofilaria immitis).many species of filarial nematodes are infected with wolbachia pipientis, a maternally inherited endosymbiont. in addition to manipulating host reproduction, these bacteria also affect the evolution of the mitochondrial dna with which they are transmitted. selective sweeps can establish a single mitochondrial lineage within a wolbachia-infected population and purge genetic diversity. while this phenomenon has been studied in insect model systems, it has not been thoroughly examined in a filarial ...201021171866
bacterial diversity of field-caught mosquitoes, aedes albopictus and aedes aegypti, from different geographic regions of madagascar.symbiotic bacteria are known to play important roles in the biology of insects, but the current knowledge of bacterial communities associated with mosquitoes is very limited and consequently their contribution to host behaviors is mostly unknown. in this study, we explored the composition and diversity of mosquito-associated bacteria in relation with mosquitoes' habitats. wild aedes albopictus and aedes aegypti were collected in three different geographic regions of madagascar. culturing methods ...201021175696
bacterial endosymbiont localization in hyalesthes obsoletus, the insect vector of bois noir in vitis vinifera.one emerging disease of grapevine in europe is bois noir (bn), a phytoplasmosis caused by "candidatus phytoplasma solani" and spread in vineyards by the planthopper hyalesthes obsoletus (hemiptera: cixiidae). here we present the first full characterization of the bacterial community of this important disease vector collected from bn-contaminated areas in piedmont, italy. length heterogeneity pcr and denaturing gradient gel electrophoresis analysis targeting the 16s rrna gene revealed the presenc ...201021183640
assessing key safety concerns of a wolbachia-based strategy to control dengue transmission by aedes mosquitoes.mosquito-borne diseases such as dengue fever, chikungunya or malaria affect millions of people each year and control solutions are urgently needed. an international research program is currently being developed that relies on the introduction of the bacterial endosymbiont wolbachia pipientis into aedes aegypti to control dengue transmission. in order to prepare for open-field testing releases of wolbachia-infected mosquitoes, an intensive social research and community engagement program was unde ...201021225190
proton beam therapy of uveal melanomas. 20103006647
wolbachia infection lowers fertile sperm transfer in a moth.the endosymbiotic bacterium wolbachia pipientis manipulates host reproduction by rendering infected males reproductively incompatible with uninfected females (cytoplasmic incompatibility; ci). ci is believed to occur as a result of wolbachia-induced modifications to sperm during maturation, which prevent infected sperm from initiating successful zygote development when fertilizing uninfected females' eggs. however, the mechanism by which ci occurs has been little studied outside the genus drosop ...201020880864
metaphyseal dysostosis schmidt type. 20103251840
myths that threaten. 20103380485
proton beam therapy of uveal melanomas. 20103006647
transport of light in tissue in photodynamic therapy. 20102964653
therapeutic drug monitoring. 20102863512
acute humoral rejection of an abo-unmatched liver allograft in a pediatric recipient.acute humorally mediated rejection of organ allografts has long been recognized in kidney transplantation as the syndrome of hyperacute rejection. this reaction precludes renal transplantation across the major blood-group system and in many other instances in which preformed antibodies are present in the host. there is increasing evidence of the importance of humoral rejection in graft survival following liver transplantation. although the antibodies responsible for this process have seldom been ...20103067237
acute humoral rejection of an abo-unmatched liver allograft in a pediatric recipient.acute humorally mediated rejection of organ allografts has long been recognized in kidney transplantation as the syndrome of hyperacute rejection. this reaction precludes renal transplantation across the major blood-group system and in many other instances in which preformed antibodies are present in the host. there is increasing evidence of the importance of humoral rejection in graft survival following liver transplantation. although the antibodies responsible for this process have seldom been ...20103067237
Refresh
firstprev
123456789
nextlast
Displaying items 401 - 500 of 827