Publications

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serendipitous discovery of wolbachia genomes in multiple drosophila species.the trace archive is a repository for the raw, unanalyzed data generated by large-scale genome sequencing projects. the existence of this data offers scientists the possibility of discovering additional genomic sequences beyond those originally sequenced. in particular, if the source dna for a sequencing project came from a species that was colonized by another organism, then the project may yield substantial amounts of genomic dna, including near-complete genomes, from the symbiotic or parasiti ...200515774024
[polymorphism of mitochondrial dna and infection with symbiotic cytoplasmic bacterium wolbachia pipientis in mosquitoes of the culex pipiens complex from russia].a total of 208 mosquitoes of the culex pipiens complex from 15 basement and terrestrial populations collected in different regions of the european part of russia and siberia were examined by genetic methods. among these, two major mitotypes, m and p, were identified. these mitotypes differed by six substitutions in the 246-bp mitochondrial dna cytochrome oxidase i gene fragment examined. urban basement mosquito ecotype c. pipiens (form molestus) were characterized by the presence of mitotype m a ...200515865286
distribution, expression, and motif variability of ankyrin domain genes in wolbachia pipientis.the endosymbiotic bacterium wolbachia pipientis infects a wide range of arthropods, in which it induces a variety of reproductive phenotypes, including cytoplasmic incompatibility (ci), parthenogenesis, male killing, and reversal of genetic sex determination. the recent sequencing and annotation of the first wolbachia genome revealed an unusually high number of genes encoding ankyrin domain (ank) repeats. these ank genes are likely to be important in mediating the wolbachia-host interaction. in ...200516030207
can cytoplasmic incompatibility inducing wolbachia promote the evolution of mate preferences?the maternally inherited bacterium, wolbachia pipientis, manipulates host reproduction by rendering uninfected females reproductively incompatible with infected males (cytoplasmic incompatibility, ci). hosts may evolve mechanisms, such as mate preferences, to avoid fitness costs of wolbachia infection. despite the potential importance of mate choice for wolbachia population dynamics, this possibility remains largely unexplored. here we model the spread of an allele encoding female mate preferenc ...200516033569
evidence for a global wolbachia replacement in drosophila melanogaster.wolbachia are maternally inherited intracellular alpha-proteobacteria found in numerous arthropod and filarial nematode species. they influence the biology of their hosts in many ways. in some cases, they act as obligate mutualists and are required for the normal development and reproduction of the host. they are best known, however, for the various reproductive parasitism traits that they can generate in infected hosts. these include cytoplasmic incompatibility (ci) between individuals of diffe ...200516085497
[differentiation between the urban mosquito culex pipiens pipiens f. molestus and culex torrentium (diptera: culicidae) by the molecular genetic methods].the molecular characters suitable for the differentiation between the autogenous form of culex pipiens pipiens (the urban mosquito) and the species culex torrentium, which was previously identified erroneously as an anautogenous form of c. pipiens pipiens (shaikevich, vinogradova, 2004), are given. these mosquitoes differ in the infection by the endosymbiotic bacteria wolbachia pipientis, 6 transitions among 247 nucleotide sequences of 3' part of the cytochrome oxidase subunit i (coi) gene of mi ...200516396396
[sexual reproduction of insects is regulated by cytoplasmic bacteria].the effects have been considered that the intracellular symbiotic alpha-proteobacteria wolbachia pipientis induces in its hosts, such as insects and other arthropods: cytoplasmic incompatibility upon mating, feminization, parthenogenesis, and androcide. specific features of the bacterium genome and possible mechanisms of its action on hosts are discussed.200516208939
efficient procedure for purification of obligate intracellular wolbachia pipientis and representative amplification of its genome by multiple-displacement amplification.bacteria belonging to the genus wolbachia are obligatory microendocytobionts that infect a variety of arthropods and a majority of filarial nematode species, where they induce reproductive alterations or establish a mutualistic symbiosis. although two whole genome sequences of wolbachia pipientis, for strain wmel from drosophila melanogaster and strain wbm from brugia malayi, have been fully completed and six other genome sequencing projects are ongoing (http://www.genomesonline.org/index.cgi?wa ...200516269725
phylogeny of wolbachia pipientis based on glta, groel and ftsz gene sequences: clustering of arthropod and nematode symbionts in the f supergroup, and evidence for further diversity in the wolbachia tree.current phylogenies of the intracellular bacteria belonging to the genus wolbachia identify six major clades (a-f), termed 'supergroups', but the branching order of these supergroups remains unresolved. supergroups a, b and e include most of the wolbachiae found thus far in arthropods, while supergroups c and d include most of those found in filarial nematodes. members of supergroup f have been found in arthropods (i.e. termites), and have previously been detected in the nematode mansonella ozza ...200516339946
primary dental care: an educational methodology for dental hygiene students. 20051069646
[gastric secretion in gastroduodenal ulcer]. 2005545511
autosomal recessive inheritance of nager acrofacial dysostosis.nager acrofacial dysostosis is a variant of mandibulofacial dysostosis with severe micrognathia, malar hypoplasia, and radial limb defects. most cases are sporadic, but autosomal recessive inheritance has been suggested. a family is reported in which two sibs are affected by this syndrome, presenting further evidence for autosomal recessive inheritance. the recognition of this syndrome as a distinct entity has important implications. after the birth of a child with orofacial malformations sugges ...20053367347
[vascular surgery in the aged]. 2005538948
[gastroenterological disorders in diabetes mellitus. iii. other disorders]. 2005545514
phobic response factors from the fear survey schedule.a phobic response scale was factored from responses to the fear survey schedule. subjects were 137 male and 168 female college students. a six factor solution extracted 41.78% of the trace. the factors were as follows: i (hostile-dependence), ii(body assult), iii (developmental fears), iv (performance and evaluation), v (death evasion), and vi (nuisance animals). an analysis of sex differences by item and scale generally supported and extended previous research. some conceptual ties were made by ...20051151900
[resection of chondrosarcoma of the sternum with restoration of the thoracic wall. report of a case]. 20051081246
[hepatotoxicity of cyproterone acetate]. 20051410910
[repeated cesarean section. report of 100 cases]. 20051175985
cancer communiqué. encore--a new approach to rehabilitation. 20051175846
academic freedom. 20051069648
secretogranin i/chromogranin b is a heparin-binding adhesive protein.the major heparin-binding protein secreted by pc12 cells was purified from conditioned medium. amino-terminal sequencing of the purified protein identified it as secretogranin i/chromogranin b (sgi/chmb). the protein showed the same electrophoretic mobility and biochemical characteristics as previously reported for sgi/chmb and could be purified in high yield using a simple procedure. in vitro experiments demonstrated that sgi/chmb effectively promoted cell-substratum adhesion of nih 3t3 and pc1 ...20051560226
the relationship of pretreatment family functioning to drinking behavior during follow-up by alcoholic patients.the relationship of alcoholics' perceptions of the pretreatment functioning of their families to drinking outcomes during an 18-month follow-up was examined. family functioning was hypothesized to be predictive of drinking behavior, particularly in subjects with low assertion of autonomy scores. these individuals report greater dependency and attachment, and therefore might be more affected by the state of important relationships. the results indicated that with low autonomy male alcoholics, gre ...20051449124
considerations in modifying a semiquantitative food frequency questionnaire.quantifying dietary intake remains a challenge. although advances have been made, we are still attempting to find a quick yet reliable intake method. utilizing brief, frequency-type questionnaires that have already been tested is an attractive option. however, many of us would also like to alter those questionnaires to make them more specific to our study population. the items covered in this article should help to illuminate some of the tissues that must be considered.20061894908
considerations in modifying a semiquantitative food frequency questionnaire.quantifying dietary intake remains a challenge. although advances have been made, we are still attempting to find a quick yet reliable intake method. utilizing brief, frequency-type questionnaires that have already been tested is an attractive option. however, many of us would also like to alter those questionnaires to make them more specific to our study population. the items covered in this article should help to illuminate some of the tissues that must be considered.20061894908
effects of picture referencing on pvc chair, love seat, and settee assemblies by students with mental retardation.this study examined the effects of two indirect corrective feedback procedures on the assembly skills of five secondary students with moderate mental retardation. picture and video referencing conditions, during which the experimenters pointed to a picture or video screen following a performance error and requested the student do the step again, were more effective than assembly photographs, sequenced pictures, sequenced pictures and modeling, and video modeling conditions. picture referencing e ...20061626083
[critical intra-abdominal infections: emerging concepts]. 20061592313
package simplifies compliance with federal regulations. 20061636208
glomerular immune deposits in murine lupus models may contain histones.two types of lupus mice, nzb/nzw f1 female hybrids and mice with graft-versus-host disease (gvhd), were studied. histones h3 and h2a were detected by immunofluorescence in glomeruli of 22/22 proteinuric gvhd and 8/12 proteinuric nzb/w f1 female mice; in non-proteinuric animals, 3/5 gvhd and 2/27 nzb/w f1 female were positive. using antibodies to histone peptides it was shown that mainly the n-terminal regions of histones h3 and h2a were exposed in glomerular deposits. western blot analysis revea ...20061458682
[digestive hemorrhage caused by aorto-duodenal fistula. report of 5 cases].the aortoduodeonal fistula is a rare condition and has high mortality. we report five retrospective cases of digestive hemorrhage due to an aortoduodenal fistula (4 secondary and 1 primary) between 1986 and 1990. three of them died. attention is called to the importance of clinical suspicion and the main diagnostic methods in early diagnosis such as oral endoscopy. we also enhance the importance of emergency surgery for survival.20061599763
[facing adversity with optimism. interview by kjell arne bakke]. 20061489601
role of technetium 99m-labeled monoclonal antibody in the management of melanoma patients.a study was performed to assess the usefulness of a radiolabeled monoclonal antibody (moab; technetium 99m nr-ml-05 fab) as a detecting agent, as well as to evaluate its role in the overall decision-making process in the management of cutaneous malignant melanoma.20061634923
analysis of viral pathogenesis by in situ hybridization. 20061560322
shoulder dystocia. 20061560295
increased expression of beta-amyloid protein precursor and microtubule-associated protein tau during the differentiation of murine embryonal carcinoma cells.expression of the genes encoding the beta/a4 amyloid protein precursor (app) and microtubule-associated protein tau was studied in an embryonal carcinoma cell line (p19) that differentiates in vitro into cholinergic neurons after treatment with retinoic acid. expression of app increased 34- (mrna) and 50-fold (protein) during neuronal differentiation; app-695 accounted for most of this increase. these remarkable increases in app expression coincided with a proliferation of neuronal processes and ...20061560239
influence of alcohol intake on the course and consequences of spinal cord injury. 20061428390
[immenkamp inactivator plate and schulte interceptor]. 20061426454
[application of the isler and ganz classification of pelvic ring fractures in clinical practice].the classification of pelvic fractures proposed by isler and ganz was applied to 152 patients. on the basis of this experience, the following conclusion were drawn. 1. it is possible to classify all pelvic ring injuries by dividing them in lesions of the anterior and of the posterior ring segment. 2. the classification requires a thorough examination; often a ct scan is needed. the original classification of 9 out of 18 patients based on x-ray morphology had to be revised after ct examination. 3 ...20061361709
nutrition and head start. 20061458961
supporting collaboration through a nursing informatics curriculum stage ii.collaboration is at the center of the process used to design, implement and evaluate an integrated informatics curriculum in a baccalaureate nursing program. this paper describes the second stage of a process to design the informatics nursing courses. the challenges to foster faculty collaborative relationships as well as to enhance the course content of all nursing informatics curriculum. a number of strategies were used to develop the collaborative efforts between the faculty and nursing staff ...20061482910
an enhanced-response system for performing chemiluminescent and bioluminescent tests. 20061366493
automated tumor registry for oncology. a va-dhcp mumps application.the va automated tumor registry for oncology, version 2, is a multifaceted, completely automated user-friendly cancer database. easy to use modules include: automatic casefinding; suspense files; abstracting and printing; follow-up; annual reports; statistical reports; utility functions.20061482866
community action research teams in population focused nursing a post diploma learning experience. 20061636365
chondromyxoid fibroma of the mandible. diagnostic image cytometry findings and review of the literature.a case of chondromyxoid fibroma in the mandible of a 12-year-old boy is reported. a review of the literature is presented with emphasis on flow and image cytometric analysis and evaluation of the radiologic and histologic differences between chondromyxoid fibroma and chondrosarcoma.20061574309
child abuse: does prosecution serve any useful purpose? 20061635513
[the basic groups of modern preparations. ii. loop diuretics. osmotic diuretics. aquaretics. uricosuric diuretics]. 20061440324
[long-term outcome of a hospital series of patients with atrio-ventricular accessory pathway].ninety five patients with a mean age of 39 +/- 19 years, 82 of whom were symptomatic, having an accessory atrioventricular bidirectional conduction pathway (wpw syndrome: 77; "concealed": 18) were followed up for an average of 7.3 +/- 2.6 years. the objectives were to analyse: the incidence and causes of death and the possible predictive factors of death due to the wpw syndrome--the influence of medical treatment and type of medication on survival and symptoms. of the 8 cardiac deaths, 6 seemed ...20061363771
delusional reduplication following closed-head injury.somatic delusions following brain injury are not uncommon, and have been well documented in the literature. this study documents a case of somatic delusion which was seen in a patient following a head injury secondary to a motorcycle accident. although perhaps not typical it serves to illustrate an interesting example of a somatic delusion following head trauma. on recovery from coma this patient reported the existence of a 'third arm' adjacent to the limb that had received the greatest impact i ...20061393179
wolbachia infection reduces sperm competitive ability in an insect.the maternally inherited bacterium wolbachia pipientis imposes significant fitness costs on its hosts. one such cost is decreased sperm production resulting in reduced fertility of male drosophila simulans infected with cytoplasmic incompatibility (ci) inducing wolbachia. we tested the hypothesis that wolbachia infection affects sperm competitive ability and found that wolbachia infection is indeed associated with reduced success in sperm competition in non-virgin males. in the second male role, ...200616777737
feminizing wolbachia in zyginidia pullula (insecta, hemiptera), a leafhopper with an xx/x0 sex-determination system.zyginidia pullula is a grass-dwelling leafhopper characterized by a bisexual reproduction mode. in this species, some females collected in northern italy, when mated with males, gave origin to an exclusively female brood. here, we demonstrated that in these lineages an infection by a new strain of wolbachia pipientis-designated as wzygpul-was detected by amplifying and sequencing the wsp and 16s rrna genes. about half of the female progeny were characterized by intersexual phenotypes, i.e. showi ...200616928646
rate of elimination of wolbachia pipientis by doxycycline in vitro increases following drug withdrawal.wolbachia pipientis is an obligate intracellular bacterium within the family anaplasmataceae that infects many terrestrial arthropods and arthropod-transmitted nematodes (filariae). several filarial species are major human pathogens, and antibiotics with activity against wolbachia offer a promising new therapeutic approach, since the adult worms are relatively refractory to conventional anthelmintics but depend on wolbachia for reproduction and viability. in a natural filarial parasite of cattle ...200616495252
wolbachia endosymbiotic bacteria of brugia malayi mediate macrophage tolerance to tlr- and cd40-specific stimuli in a myd88/tlr2-dependent manner.lymphatic filarial nematodes are able to down-regulate parasite-specific and nonspecific responses of lymphocytes and apc. lymphatic filariae are reliant on wolbachia endosymbiotic bacteria for development and survival. we tested the hypothesis that repeated exposure to wolbachia endosymbionts would drive macrophage tolerance in vitro and in vivo. we pre-exposed murine peritoneal-elicited macrophages to soluble extracts of brugia malayi female worms (bmfe) before restimulating with bmfe or tlr a ...200616818783
multilocus sequence typing system for the endosymbiont wolbachia pipientis.the eubacterial genus wolbachia comprises one of the most abundant groups of obligate intracellular bacteria, and it has a host range that spans the phyla arthropoda and nematoda. here we developed a multilocus sequence typing (mlst) scheme as a universal genotyping tool for wolbachia. internal fragments of five ubiquitous genes (gatb, coxa, hcpa, fbpa, and ftsz) were chosen, and primers that amplified across the major wolbachia supergroups found in arthropods, as well as other divergent lineage ...200616936055
asymmetrical interactions between wolbachia and spiroplasma endosymbionts coexisting in the same insect host.we investigated the interactions between the endosymbionts wolbachia pipientis strain wmel and spiroplasma sp. strain nsro coinfecting the host insect drosophila melanogaster. by making use of antibiotic therapy, temperature stress, and hemolymph microinjection, we established the following strains in the same host genetic background: the sw strain, infected with both spiroplasma and wolbachia; the s strain, infected with spiroplasma only; and the w strain, infected with wolbachia only. the infe ...200616820474
survival of wolbachia pipientis in cell-free medium.wolbachia pipientis is an obligate intracellular bacterium found in a wide range of invertebrate taxa. while over ecological timescales wolbachia infections are maintained by strict maternal inheritance, horizontal transfer events are common over evolutionary time. to be horizontally transferred between organisms, wolbachia bacteria must pass through and survive an extracellular phase. we used baclight live-dead staining, pcr, and fluorescence in situ hybridization to assess the ability for puri ...200616950898
can anopheles gambiae be infected with wolbachia pipientis? insights from an in vitro system.wolbachia pipientis are maternally inherited endosymbionts associated with cytoplasmic incompatibility, a potential mechanism to drive transgenic traits into anopheles populations for malaria control. w. pipientis infections are common in many mosquito genera but have never been observed in any anopheles species, leading to the hypothesis that anopheles mosquitoes are incapable of harboring infection. we used an in vitro system to evaluate the ability of anopheles gambiae cells to harbor diverse ...200617028229
toward a wolbachia multilocus sequence typing system: discrimination of wolbachia strains present in drosophila species.among the diverse maternally inherited symbionts in arthropods, wolbachia are the most common and infect over 20% of all species. in a departure from traditional genotyping or phylogenetic methods relying on single wolbachia genes, the present study represents an initial multilocus sequence typing (mlst) analysis to discriminate closely related wolbachia pipientis strains, and additional data on sequence diversity in wolbachia. we report a new phylogenetic characterization of four genes (aspc, a ...200617036209
increased male mating rate in drosophila is associated with wolbachia infection.the maternally inherited bacterium wolbachia pipientis infects 25-75% of arthropods and manipulates host reproduction to improve its transmission. one way wolbachia achieves this is by inducing cytoplasmic incompatibility (ci), where crosses between infected males and uninfected females are inviable. infected males suffer reduced fertility through ci and reduced sperm production. however, wolbachia induce lower levels of ci in nonvirgin males. we examined the impact of wolbachia on mating behavi ...200617040394
cough variant asthma: usefulness of a diagnostic-therapeutic trial with prednisone.cough variant asthma is characterized as a persistent, nonproductive cough with minimal or no wheezing and dyspnea. the diagnosis can be overlooked or misdisagnosed. we describe the severity of cough, the misery of some patients who have this syndrome and the usefulness of a diagnostic-therapeutic trial in ten patients with cough variant asthma. we evaluated ten patients whose chief complaint was persistent nonproductive cough. during the course of evaluation, all patients received a diagnostic- ...20061471782
disruption of the wolbachia surface protein gene wspb by a transposable element in mosquitoes of the culex pipiens complex (diptera, culicidae).culex pipiens quinquefasciatus say and culex pipiens pipiens linnaeus are sibling species incriminated as important vectors of emerging and re-emerging infectious diseases worldwide. the two forms differ little morphologically and are differentiated mainly based upon ecological, behavioural, physiological and genetic traits. within the north american zone of sympatry, populations of cx. p. quinquefasciatus and cx. p. pipiens undergo extensive introgression and hybrid forms have been reported in ...200717298560
taxonomic status of the intracellular bacterium wolbachia pipientis.wolbachia pipientis is a maternally inherited, intracellular bacterium found in more than 20 % of all insects, as well as numerous other arthropods and filarial nematodes. it has been the subject of a growing number of studies in recent decades, because of the remarkable effects it has on its arthropod hosts, its potential as a tool for biological control of arthropods of agricultural and medical importance and its use as a target for treatment of filariasis. w. pipientis was originally discover ...200717329802
anthraquinones as defensive compounds in eggs of galerucini leaf beetles: biosynthesis by the beetles?eggs of leaf beetles of the tribe galerucini, subfamily galerucinae, contain polyketides that are unusual in insects: 1,8-dihydroxylated anthraquinones (chrysazin, chrysophanol) and anthrones (dithranol, chrysarobin) deterring predators. the host plants do not contain these compounds. in the present study, we tested the hypothesis that the beetles, but not bacterial or fungal microorganisms living as endosymbionts within the beetles, produce the anthraquinones. the tansy leaf beetle galeruca tan ...200717879233
symbiont genes in host genomes: fragments with a future?while lateral transfer is the rule in the evolutionary history of bacterial and archaeal genes, events of transfer from prokaryotes to eukaryotes are rare. germline-transmitted animal symbionts, such as wolbachia pipientis, are well placed to participate in such transfers. in a recent issue of science, dunning hotopp et al. identified instances of transfer of wolbachia dna to host genomes. it is unknown whether these transfers represent innovation in animal evolution.200718005738
f supergroup wolbachia in bush crickets: what do patterns of sequence variation reveal about this supergroup and horizontal transfer between nematodes and arthropods?wolbachia pipientis, an intracellular, alpha-proteobacterium, is commonly found in arthropods and filarial nematodes. most infected insects are known to harbor strains of wolbachia from supergroups a or b, whereas supergroups c and d occur only in filarial nematodes. here, we present molecular evidence from two genes (ftsz and 16s rdna) that 2 orthopterans (the bush cricket species orocharis saltator and hapithus agitator; gryllidae: eneopterinae) are infected with wolbachia from the f supergrou ...200716924406
population replacement strategies for controlling vector populations and the use of wolbachia pipientis for genetic drive.in this video, jason rasgon discusses population replacement strategies to control vector-borne diseases such as malaria and dengue. "population replacement" is the replacement of wild vector populations (that are competent to transmit pathogens) with those that are not competent to transmit pathogens. there are several theoretical strategies to accomplish this. one is to exploit the maternally-inherited symbiotic bacteria wolbachia pipientis. wolbachia is a widespread reproductive parasite that ...200718979023
diversifying selection and host adaptation in two endosymbiont genomes.the endosymbiont wolbachia pipientis infects a broad range of arthropod and filarial nematode hosts. these diverse associations form an attractive model for understanding host:symbiont coevolution. wolbachia's ubiquity and ability to dramatically alter host reproductive biology also form the foundation of research strategies aimed at controlling insect pests and vector-borne disease. the wolbachia strains that infect nematodes are phylogenetically distinct, strictly vertically transmitted, and r ...200717470297
new criteria for selecting the origin of dna replication in wolbachia and closely related bacteria.the annotated genomes of two closely related strains of the intracellular bacterium wolbachia pipientis have been reported without the identifications of the putative origin of replication (ori). identifying the ori of these bacteria and related alpha-proteobacteria as well as their patterns of sequence evolution will aid studies of cell replication and cell density, as well as the potential genetic manipulation of these widespread intracellular bacteria.200717584494
on the taxonomic status of the intracellular bacterium wolbachia pipientis: should this species name include the intracellular bacteria of filarial nematodes? 200717684235
widespread lateral gene transfer from intracellular bacteria to multicellular eukaryotes.although common among bacteria, lateral gene transfer-the movement of genes between distantly related organisms-is thought to occur only rarely between bacteria and multicellular eukaryotes. however, the presence of endosymbionts, such as wolbachia pipientis, within some eukaryotic germlines may facilitate bacterial gene transfers to eukaryotic host genomes. we therefore examined host genomes for evidence of gene transfer events from wolbachia bacteria to their hosts. we found and confirmed tran ...200717761848
common risk factors in the development of cataract among arabs residing in kuwait. 20071868952
bone marrow biopsy in myelodysplastic syndromes: morphological characteristics and contribution to the study of prognostic factors.ten characteristics of bone marrow (bm) biopsies in paraffin sections, obtained at diagnosis from patients with myelodysplastic syndromes (mds) classified according to the fab criteria, were analysed to identify both the most relevant morphologic data and any possible influence on survival. agreement between two observers was obtained for 94% of the data. bm cellularity was increased in 63% of the cases and was higher in refractory anaemia with excess of blasts (raeb). raeb in transformation (ra ...20072375920
[evolution from retrotransposons to retroviruses: origin of the env gene].in genome of drosophila melanogaster, various families of retrotransposons with different combination of functional domens and mechanisms of transposition are present. however only retrotransposons of gypsy family are retroviruses related to errantiviruses. other families seemingly appeared as intermediate forms of retroviruses evolution. despite the fact that the question on origin of retroviruses remains unclear, now the hypothesis of their origin from retrotransoposons can be considered the m ...200718257289
transfection of wolbachia pipientis into drosophila embryos.wolbachia is a genus of obligate intracellular alpha-proteobacteria represented by the type species wolbachia pipientis (dumler et al., 2001). wolbachia commonly reside within cytoplasmic vacuoles of arthropods and helminths (werren and windsor, 2000; casiraghi et al., 2004); vertebrate infections have not been identified. wolbachia are maternally transmitted from mothers to offspring though the embryonic cytoplasm. wolbachia are able to induce a diverse range of phenotypes in their invertebrate ...200718770621
rolling chair sign. 20072241609
the mhc-binding and gp120-binding functions of cd4 are separable. 20072387122
magnetic resonance imaging in infants exposed to cocaine prenatally: a preliminary report. 20071914354
objectives to direct the training of emergency medicine residents on off-service rotation: otolaryngology.this is the sixth article in a continuing series on objectives for emergency medicine training; otolaryngological objectives will be presented. otolaryngological skills and knowledge areas are frequently encountered in clinical practice. the core content in emergency medicine devotes an entire section to their listing. off-service rotations provide some one-half of all rotational-based experience in emergency medicine training. specific behaviorally based objectives for mastery of knowledge and ...20072045654
therapy-related leukaemia in wegener's granulomatosis. 20071934930
double-blind study with fenticonazole or bifonazole lotions in pityriasis versicolor.the efficacy of fenticonazole 2% lotion was studied in patients suffering from pityriasis versicolor in a double-blind comparison with bifonazole 1% lotion, both applied once daily for three weeks. forty-six patients were recruited and randomly allocated to one or other treatment group. clinical and mycological examinations, performed at baseline and at weekly intervals, have shown an excellent response to both treatments. two cases of mild transient desquamation were reported in the fenticonazo ...20073182117
skeletal applications of videofluoroscopy. 20071919376
school policies and programs on smoking and health--united states, 1988. 20072493573
phosphoglucomutase-1 polymorphism among chinese in taiwan.phosphoglucomutase-1 (pgm1) phenotyping of 1,128 chinese blood donors was performed by thin-layer isoelectric focusing on agarose. the pgm1 gene frequencies were: 1a, 0.6005; 1b, 0.1500; 2a, 0.1510; 2b, 0.0973, and rare variants, 0.0058. the rare variants found in this series were pgm1 w21, w2, w3, w6 and w9 (or w10) with pgm1 w21 being the most common variant among chinese with a phenotype frequency of 0.8%.20071828778
contracts for rmos in obstetrics and gynaecology. 20071852332
chemical contamination in office buildings. 20071951046
allergic reaction of the oral mucosa in a patient with a synthetic denture. 20071868728
epidural morphine, hypertension, and aortic surgery. 20082570289
the emergence of a health insurance system in a developing country: the case of south korea.in an attempt to understand the social forces and the economic and political conditions under which new social policies emerge in developing countries, this study outlines factors affecting the introduction of the health insurance system in south korea. the emergence of the south korean health insurance system was influenced by changing labor needs of the industrial sector, increasing social expectations, external and international pressures, increasing medical costs, and class conflict. these p ...20082600387
expression of lymphocyte activation markers in benign cutaneous t cell infiltrates. discoid lupus erythematosus versus lichen ruber planus.the expression of lymphocyte activation markers (il2 receptors, transferrin receptors and hla-dr) was examined in cutaneous lymphoid infiltrates of 12 patients with lichen ruber planus (lp) and 10 individuals with discoid lupus erythematosus (dle). the cell infiltrates in both conditions were generally of considerable size. the vast majority of the infiltrating cells were t cells. the reactivity of the anti-il2 receptor antibody used was confined to lymphocytes. in patients with lp 26 +/- 17% of ...20082568045
[physiology of the continence organ before and following pouch-anal anastomosis]. 20082474969
altered sex ratios. 20082570947
fine-structural aspects of bromodeoxyuridine incorporation in sister chromatid differentiation and replication banding.the structure of harlequin-stained chromosomes following substitution with low levels of 5-bromodeoxyuridine (brdurd) over two cell cycles and high levels over the last part of one cycle (replication banding) was studied in chinese hamster ovary (cho) cells. by using correlative light (lm) and scanning electron microscopy (sem), it was shown that the effects of both the ultraviolet light (u.v.) and hot ssc treatment steps of the harlequin staining procedure were necessary to obtain sister-chroma ...20082621225
cystoid macular edema: a postoperative complication.cystoid macular edema (cme) is a condition of the macula characterized by an accumulation of fluid in the outer plexiform layer of henle, causing edema and cyst-like formations. the major presenting symptom of visually significant cme is a decrease in visual acuity; a fundus examination may or may not reveal the presence of cme. fluorescein angiography is used to confirm the presence of suspected cme. most cases of cme improve after a course of indomethacin; but the patient must continue to be f ...20082374166
ovarian and adrenal function in the parthenogenetic whiptail lizard cnemidophorus uniparens in the field and laboratory.the purpose of this study was to document circulating concentrations of corticosterone in the plasma in relation to the ovarian follicular cycle in animals in the field and to examine the effects of captivity and laboratory manipulations on plasma corticosterone in the parthenogenetic whiptail lizard cnemidophorus uniparens. in nature, circulating concentrations of corticosterone are highest in previtellogenic individuals when concentrations of progesterone are basal and estradiol-17 beta is und ...20082583474
maturation of hematolymphoid cells that express thy-1. 20082577321
[statistical evaluation of 4,141 osteosyntheses with autocompression plates].statistical display of the cases of our hospital who underwent osteosynthesis with autocompression plate between 1968 and 1986. based on the unselected evaluation of our surgical reports and computer monitoring an analysis of the total of 4.141 acp-osteosynthesis is given according to the various origin types of surgical procedures, age and sex distribution and chiefly complications of the operative revisions. the total of revised complications was 4.18%, among that the rate of infections was 1. ...20082565651
plasma cholesterol, coronary heart disease, and cancer. 20082500179
porcelain laminate veneers--for improved aesthetics of anterior teeth. 20082605818
origin and organization of the zebrafish fate map.we have analyzed lineages of cells labeled by intracellular injection of tracer dye during early zebrafish development to learn when cells become allocated to particular fates during development, and how the fate map is organized. the earliest lineage restriction was described previously, and segregates the yolk cell from the blastoderm in the midblastula. after one or two more cell divisions, the lineages of epithelial enveloping layer (evl) cells become restricted to generate exclusively perid ...20082387237
morbidity and mortality of stapled anastomoses on a gynecologic oncology service: a retrospective review.the gynecologic oncology division performed 167 operations requiring intestinal resection during the period july 1, 1980 to may 30, 1989. stapling instruments were utilized in 155 procedures and resulted in 204 anastomoses. major morbidity occurred in 36 (23%) patients, including 17 (11%) deaths and (4.5%) fistulas. minor morbidity, such as ileus and atelectasis, occurred in 46 (30%) patients. intestinal surgery is required in approximately 10% of gynecologic oncology procedures, and is associat ...20082323618
[impressions for removable partial dentures]. 20082203625
hyponatraemia in patients with cirrhosis.hyponatraemia occurs in nearly half of patients in hospital with cirrhosis and ascites, and is due to the excessive retention of free water which results from the kidney's inability to excrete it normally. the morbidity and mortality associated with hyponatraemia is largely attributable to central nervous system disturbances. the degree to which brain water content increases depends on the duration of hyponatraemia and on compensatory mechanisms. altered steroid and peptide hormones in cirrhotic ...20082217672
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