| transfer of kingella indologenes (snell and lapage 1976) to the genus suttonella gen. nov. as suttonella indologenes comb. nov.; transfer of bacteroides nodosus (beveridge 1941) to the genus dichelobacter gen. nov. as dichelobacter nodosus comb. nov.; and assignment of the genera cardiobacterium, dichelobacter, and suttonella to cardiobacteriaceae fam. nov. in the gamma division of proteobacteria on the basis of 16s rrna sequence comparisons. | the 16s rrna sequences of kingella indologenes, cardiobacterium hominis, and bacteroides nodosus were determined by direct rna sequencing, using a modified sanger method. sequence comparisons indicated that these three species represent a novel family in the gamma division of proteobacteria. on the basis of these data, k. indologenes and b. nodosus cannot retain their current generic status as they are not closely related to other members of their assigned genera. therefore, we propose transfer ... | 1990 | 2275858 |
| additional dna long-range folding is an early process during stimulation of human lymphocytes. | there is disagreement about the existence of permanent single-strand breaks in the nuclear dna of quiescent lymphocytes, possibly due to different experimental approaches. using the nucleoid sedimentation technique, no evidence is found for spontaneous dna single-strand breakage in unstimulated human lymphocytes and for quick break-sealing after mitogenic stimulation. an increased sedimentation rate of nucleoids, which can be monitored as soon as 3 h after stimulation, is shown to be due rather ... | 1990 | 2347375 |
| serum antibody responses in sheep after natural infection with bacteroides nodosus. | soluble outer membrane protein of bacteroides nodosus extracted with potassium thiocyanate (kscn) was employed as antigen in an enzyme linked immunosorbent assay (elisa) to detect serum antibody in sheep naturally infected with a heterologous serogroup. serum antibody responses in 55 sheep were monitored for 2 years and maximum levels were directly related to the severity of clinical foot lesions. serum antibody levels rose 2 weeks after foot lesions developed and declined within several months ... | 1990 | 2375715 |
| an anamnestic serological test for ovine footrot. | following recovery from ovine footrot, a proportion of sheep in a flock may carry the causative organism and spread it to other sheep if environmental conditions are favourable. footrot affected sheep have elevated levels of serum antibody against bacteroides nodosus, but these levels decline rapidly after clinical recovery. when challenged by subcutaneous injection with 470 micrograms of protein extracted from the cell membrane of b. nodosus, without adjuvant, sheep that had recovered clinicall ... | 1990 | 2378598 |
| variation in the structural subunit and basal protein antigens of bacteroides nodosus fimbriae. | the fimbriae of bacteroides nodosus play a major role in protective immunity against ovine footrot and are an important determinant in the serological classification system that divides field isolates into at least eight serogroups and 16 serotypes. purified fimbriae contain two polypeptide antigens, the structural subunit of the fimbrial strand (molecular weight about 17,000) and a basal protein (molecular weight about 80,000), both of which exhibit structural variation. fimbriae were prepared ... | 1986 | 2422154 |
| effect of endogenous and exogenous interferons on the differentiation of human monocyte cell line u937. | the effect of interferons (ifns) on the differentiation of hematopoietic cells was examined with the human monocyte cell line u937. the differentiation of u937 was induced by hydroxyvitamin d3 and was evaluated through the study of specific markers. the induction of the u937 differentiation was associated with a production of ifn and with a marked increase in (2'5') oligoadenylate synthetase. addition of anti-ifn-alpha/beta antibodies inhibited the enhancement of (2'5') oligoadenylate synthetase ... | 1988 | 2446743 |
| monoclonal antibodies defining immunogenic regions of pili from bacteroides nodosus strains 198 (a1), 265 (h1) and 336 (f1). | a total of 17 monoclonal antibodies (moab) were used to analyse the antigenic structure of pilus protein from three serogroups of bacteroides nodosus. the four moab which agglutinated pili were serogroup (and subgroup) specific, and the agglutinating epitope was present on the pili monomer and dependent on the intra-chain disulfide bond. non-agglutinating moab identified two further non-linear and serogroup-restricted epitopes on strain 198 (a1) pili and two linear epitopes on 336 (f1) and 265 ( ... | 1989 | 2470668 |
| generation, maintenance and reactivity of ovine t-lymphocyte clones derived from sheep immunized with pili from bacteroides nodosus. | peripheral blood leucocytes (pbl) from sheep immunized with pilus protein purified from bacteroides nodosus serogroup a were cultivated in vitro and cloned in the presence of the specific antigen and autologous antigen-presenting cells (apc). the efficiency of cloning was enhanced by high proliferative responses to pili during the initial week of cultivation, and the provision of recombinant human interleukin-2 (rec-il-2). after three passages at weekly intervals, bulk cultures of pbl and cloned ... | 1989 | 2477941 |
| fimbriae of bacteroides nodosus: protein engineering of the structural subunit for the production of an exogenous peptide. | the pattern of sequence variation between bacteroides nodosus fimbrial subunits of different serotypes suggests a degree of flexibility, which might be exploited for protein engineering approaches for the expression of other peptides. we have tested this using the well-characterized peptide epitope from vp1 of foot-and-mouth disease virus (fmdv), residues 144-159: lrgdlqvlaqkvartl (strain 01-bfs). using bacterial codon usage, several oligonucleotides were designed for the substitution of this se ... | 1989 | 2564674 |
| amino acid sequences of pilins from serologically distinct strains of bacteroides nodosus. | amino acid sequences of pilin from a strain of bacteroides nodosus from serogroup b (234) and serogroup c (217) were determined. the amino-terminal n-methylphenlalanine residue of both proteins was followed by a hydrophobic sequence of 30 residues closely related to the n-terminal sequence of other pili having an amino-terminal residue of n-methylphenylalanine. these data lend support to the hypothesis that in pilins of this type, the amino-terminal sequence functions as a transport signal neces ... | 1988 | 2577730 |
| increased prostaglandin synthesis in the unclipped kidney of one-kidney, one clip hypertensive rats. | reversal of one-kidney, one clip (1-k, 1c) hypertension by removal of the renal artery clip is accompanied by increased renal and vascular prostaglandin (pg) production. it was postulated that pg biosynthesis is stimulated in the unclipped hypertensive kidney. in order to test this hypothesis, we compared urinary excretion of pge2 and 6-keto-pgf1 alpha (a breakdown product of pgi2) in perfused kidneys isolated from 1-k, 1c hypertensive rats, 1-k, sham-clipped rats and 1-k, 1c rats which had fail ... | 1989 | 2607143 |
| sequence of pilin from bacteroides nodosus 351 (serogroup h) and implications for serogroup classification. | the nucleotide sequence of the pilin gene from bacteroides nodosus strain 351, currently classified as serogroup h, subgroup 2 (h2) has been determined. the gene encodes a single polypeptide (prepilin) of 160 amino acids and mr 17,150. however, pilin isolated from b. nodosus 351 migrates as two distinct bands in sodium dodecyl sulphate-polyacrylamide gel electrophoresis, due to an internal peptide bond cleavage. amino acid sequence studies of pilin from b. nodosus 351 have established that the c ... | 1989 | 2621448 |
| molecular analysis of one of multiple protease-encoding genes from the prototype virulent strain of bacteroides nodosus. | the aim of these studies was to examine the organization of the bacteroides nodosus protease-encoding gene(s). the extracellular serine proteases (38 kda) from the prototype virulent strain of b. nodosus were purified and used to raise a specific antiserum in rabbits. this antiserum was used in a colony immunoassay to screen a genomic dna library constructed in escherichia coli using bamhi-digested b. nodosus dna and the plasmid pbr322. an e. coli clone expressing a 50-kda immunoreactive polypep ... | 1989 | 2666262 |
| [therapeutic implication of plasma c3 rich fraction against bacterial infection in surgical patients]. | the depleted plasma opsonic activity is a major provoking cause of severe postoperative infection in patients who underwent extended surgical procedures. in order to establish the therapeutic use of c3 rich fraction (c3rf) for these patients in place of large amount of fresh frozen plasma (ffp) replacement, a basic study was done dealt with this fraction which was extracted from ffp with polyethyleneglycol. the results were as follows. (1) addition of minimum amount of c3rf in vitro sufficiently ... | 1989 | 2677664 |
| tolerance of mefloquine alone and in combination with sulfadoxine-pyrimethamine in the prophylaxis of malaria. | a randomized double blind study was performed to evaluate the tolerance and the acceptance of mefloquine alone (lariam) compared to a combined drug regimen consisting of mefloquine, sulfadoxine and pyrimethamine (msp; fansimef) in the prophylaxis of malaria. 175 europeans travelling to different malaria endemic areas received either mefloquine alone (250 mg/week) or its combination with sulfadoxine (500 mg/week) plus pyrimethamine (25 mg/week). one person taking mefloquine and two taking msp dis ... | 1989 | 2694482 |
| current management of salivary gland tumors. part 2. | the authors base their treatment of salivary gland malignancies on the size of the primary and the histopathologic diagnosis. group 1 includes smaller tumors in the t1 and t2 classification with cell types that are associated with slow growth. a parotidectomy is usually sufficient therapy for tumors in this group. group 2 contains t1 and 2 tumors with more aggressive behavior. total parotidectomy is indicated here, with postoperative radiotherapy. t3 tumors and patients with nodal metastasis or ... | 1989 | 2701414 |
| triglyceride and cholesteryl ester hydrolysis in a cell culture model of smooth muscle foam cells. | cultured rabbit aortic smooth muscle cells were converted to foam cells by exposure to sonicated lipid droplets of defined composition using an inverted culture technique. uptake of the lipid droplets by the cells was shown to be dependent on the time of exposure to the droplets and on the mass of droplets presented to the cells. a comparison of the hydrolysis of triolein and cholesteryl oleate by cells that had been exposed to isotropic lipid droplets containing equimolar amounts of the two lip ... | 1989 | 2715724 |
| a role for dna characterisation in ovine footrot. | bacteroides nodosus involved in several outbreaks of ovine footrot over a number of years were subjected to dna restriction endonuclease analysis. individual isolates were found to have characteristic bam hi profiles which permitted their accurate identification and differentiation from other isolates. bam hi profiles of b. nodosus isolates were used in epidemiological investigations involving consecutive outbreaks of footrot on individual and neighbouring farms. the relationship of given isolat ... | 1989 | 2730474 |
| effectiveness of vaccination in controlling ovine footrot. | efficacy of reducing footrot by use of a commercial vaccine was evaluated over 2 yr with 223 ewes from five crossbred genotypes. ewes were assigned randomly within genotype, age and initial footrot status to a 2 x 3 factorial array of vaccination in the 1st yr and fall vaccination, winter vaccination or control in the 2nd yr. the flock contained a single bacteroides nodosus serotype (xv) known to be highly cross-reactive with one of the vaccine serotypes (british serotype h). vaccination reduced ... | 1989 | 2737976 |
| time-resolved extrinsic fluorescence of aromatic-l-amino-acid decarboxylase. | the coenzyme-linked fluorescence of aromatic-l-amino-acid decarboxylase decays non-exponentially. the decay of both native and nabh4 reduced samples can only be fitted by two exponentials each roughly accounting for about half of the total fluorescence. denaturation of the reduced protein with 8 m urea makes the fluorescence decay mono-exponential, like that observed for the reference compound pyridoxamine-5-phosphate. an extra pyridoxyl moiety can be bound to the enzyme after incubation with ex ... | 1989 | 2752045 |
| efficacy of a multivalent bacteroides nodosus vaccine against foot rot in sheep in britain. | a multivalent bacteroides nodosus foot rot vaccine was tested by comparing the prevalence of foot rot in 317 vaccinated ewes and lambs with the prevalence in 422 unvaccinated control sheep. the vaccine conferred statistically significant protection against foot rot. the vaccine also demonstrated a therapeutic effect in animals with foot rot, after one injection. | 1989 | 2773254 |
| a positive association between resistance to ovine footrot and particular lymphocyte antigen types. | the distribution of 12 class i ovine lymphocyte antigens (ola) was examined in 4 flocks of sheep vaccinated against and/or challenged with bacteroides nodosus, the transmitting agent of footrot. in a flock of 47 corriedales in new zealand, which had been specially bred for resistance to footrot, a higher frequency (70.2%) of ola type sy6 was found compared with 42.9% in 49 unselected corriedale sheep (p = 0.001). the serum antibody response of 12 selected corriedale ewes was compared with that o ... | 1989 | 2775053 |
| extensive cerebral calcification and retinal changes in pseudohypoparathyroidism. | the case of a 41-year-old woman with cerebral calcification of a rather unusual extent is reported. this condition was associated with mental deficiency, pseudohypoparathyroidism and albright's hereditary osteodystrophy. four years later hypothyroidism was diagnosed. visual impairment and electroretinogram abnormality suggested a retinopathy involving mostly rods. despite their rarity, pseudohypoparathyroidism and albright's hereditary osteodystrophy are of major interest, since they represent t ... | 1989 | 2809648 |
| effects of the antiglucocorticoid ru 486 on adrenal function in dogs. | we studied the antiglucocorticoid effects of ru 486 given orally in doses of 5 (low), 20 (intermediate), and 50 mg/kg (high) daily for 10 days to seven female mongrel dogs. no changes in plasma acth or cortisol levels were produced by the 5 mg/kg dose. plasma acth levels increased 3-fold with both intermediate and high dosages. plasma cortisol levels rose 4-fold (p less than 0.05) within 2 days of commencing the high dose schedule and within 3 days with the intermediate dosage. plasma aldosteron ... | 1988 | 2832437 |
| effects of the antiglucocorticoid ru 486 on adrenal function in dogs. | we studied the antiglucocorticoid effects of ru 486 given orally in doses of 5 (low), 20 (intermediate), and 50 mg/kg (high) daily for 10 days to seven female mongrel dogs. no changes in plasma acth or cortisol levels were produced by the 5 mg/kg dose. plasma acth levels increased 3-fold with both intermediate and high dosages. plasma cortisol levels rose 4-fold (p less than 0.05) within 2 days of commencing the high dose schedule and within 3 days with the intermediate dosage. plasma aldosteron ... | 1988 | 2832437 |
| [human chorionic prealbumin: its identification, physicochemical properties and detection in the blood serum in trophoblastic disease]. | human chorionic prealbumin (hcpa) was immunochemically identified and isolated in pure form from immature placenta, particularly, from chorion. comparative immunodiffusion analysis shows that hcpa differs from the known placenta-specific proteins. hcpa is a glycoprotein with mw about 100 kd determined by gel-filtration and 45 kd by sds polyacrylamide gel electrophoresis. by immunodiffusion hcpa was not found in fetal and adult tissues and in biological fluids. hcpa was discovered in patients wit ... | 1988 | 2848598 |
| intracellular crystal formation in bacteroides nodosus. | an intracellular crystalline structure was found in at least two strains of bacteroides nodosus. the inclusion appeared to be associated with good growth of the organism under optimum conditions following selective passage of rough colonies containing highly fimbriate cells. several morphological forms of the inclusion were noted. indiscriminate subculture of crystal-forming cultures led to the loss of this particular property indicating a genetically controlled system of either bacterial or bac ... | 1988 | 2854148 |
| motility in relation to virulence of bacteroides nodosus. | fourteen bacteroides nodosus isolates from footrot lesions of sheep were examined microscopically and all were found to have twitching motility. the mean percentage of cells showing motility was 40% and 9% for virulent and benign strains, respectively. this corresponded with mean agar colony diameters of 17 mm and 7 mm, respectively, for these strains. two strains of intermediate virulence had values of motility and colony diameter similar to the benign strains. however, the intermediate and the ... | 1985 | 2858936 |
| fimbriae from the oral anaerobe bacteroides gingivalis: physical, chemical, and immunological properties. | circular dichroism spectra indicated the predominance of beta-sheet structure in bacteroides gingivalis fimbriae regardless of the presence of sodium dodecyl sulfate. by using a computer program, the alpha-helix, beta-sheet, and beta-turn contents and the remainder were estimated to be 0, 55, 18, and 27%, respectively, judging from the circular dichroism spectra of the fimbriae. heating for 5 min at 100 degrees c in sodium dodecyl sulfate was necessary to denature the fimbriae into their constit ... | 1985 | 2862134 |
| legionella in power station cooling waters. | | 1985 | 2862486 |
| differences between breeds of sheep in their responses to bacteroides nodosus vaccines. | in a field experiment on irrigated pasture, sheep of several breeds were vaccinated twice, subcutaneously, in the upper neck, with bacteroides nodosus vaccine containing either depiliated cells (dc vaccine), or whole, piliated cells (wc vaccine) and the responses were measured over the following 14 weeks. dc vaccine was as effective as wc vaccine in protecting against the development of foot-rot under conditions of moderate challenge, although the wc vaccine induced significantly higher pilus ag ... | 1985 | 2862860 |
| the protection given by pilus and whole cell vaccines of bacteroides nodosus strain 198 against ovine foot-rot induced by strains of different serogroups. | a highly purified pilus vaccine prepared from cells of bacteroides nodosus strain 198 provided a high level of protection against homologous challenge and small, not statistically significant, levels of protection against challenge with 4 other strains each from different serogroups. in a second experiment, a partially purified pilus vaccine from strain 198 induced significant immunity to 1 of 4 heterologous strains which were different from those used in the first experiment. in a third experim ... | 1985 | 2864040 |
| expression of the pilin gene from bacteroides nodosus in escherichia coli. | bacterial plasmids that direct the expression in escherichia coli of the pilin of bacteroides nodosus were constructed. the quantity of pilin produced was greater than that of the pilin synthesized by b. nodosus, but no surface structural pili were present; pilin was found associated with the inner membrane of e. coli. vaccination of sheep with e. coli containing pilin elicited increases in agglutinating and enzyme-linked immunosorbent assay antibody titers, which in turn were lower than the tit ... | 1986 | 2867042 |
| electrophoretic mobility of bacteroides nodosus pilus antigens. | | 1986 | 2870704 |
| nucleotide sequence of the gene encoding the two-subunit pilin of bacteroides nodosus 265. | the nucleotide sequence of the gene encoding pilin from bacteroides nodosus 265 has been determined. the pilin is encoded by a single-copy gene, from which can be predicted a prepilin comprising a single protein chain of mr 16,637. the prepilin sequence differs in several respects from the mature protein sequence. seven additional n-terminal amino acid residues are present in prepilin, whereas residue 8, phenylalanine, undergoes posttranslational modification to become the n-methylated amino-ter ... | 1986 | 2873127 |
| cross-protection from bacteroides nodosus vaccines and the interaction of pili and adjuvants. | the effects of vaccination of merino sheep with the purified pili or the whole cells of bacteroides nodosus strain 198, either in oil or alum-oil adjuvant, on the severity of foot-rot induced with the homologous strain (198) and a heterologous strain (217) were determined in a field experiment, on flood irrigated pasture. the efficacy of the whole cell vaccines was comparable to that of purified pili vaccines, against homologous challenge, when both had a similar content of pilus antigen althoug ... | 1986 | 2874786 |
| expression of pili from bacteroides nodosus in pseudomonas aeruginosa. | the pili of bacteroides nodosus, the causative agent of ovine footrot, constitute the major host-protective immunogen against homologous serotypic challenge. the pilin gene from b. nodosus 198 has been cloned and morphologically expressed as extracellular pili in pseudomonas aeruginosa by using a plasmid-borne, thermoregulated expression system. b. nodosus pilin could not be detected in cultures of p. aeruginosa grown at 32 degrees c, but after induction at 37 degrees c, b. nodosus pili were exp ... | 1986 | 2877967 |
| morphogenetic expression of bacteroides nodosus fimbriae in pseudomonas aeruginosa. | type 4 fimbriae are found in a range of pathogenic bacteria, including bacteroides nodosus, moraxella bovis, neisseria gonorrhoeae, and pseudomonas aeruginosa. the structural subunits of these fimbriae all contain a highly conserved hydrophobic amino-terminal sequence preceding a variable hydrophilic carboxy-terminal region. we show here that recombinant p. aeruginosa cells containing the b. nodosus fimbrial subunit gene under the control of a strong promoter (pl, from bacteriophage lambda) prod ... | 1987 | 2878919 |
| the pathogenicity and cultural characteristics of virulent, intermediate and benign strains of bacteroides nodosus causing ovine foot-rot. | the relationship between the cultural and biochemical characteristics of 22 strains of bacteroides nodosus and their virulence for sheep was examined. virulent, intermediate and benign strains were recognised. although there was some relationship between virulence and colony morphology on hoof medium with 4% agar, colonies of one virulent and 4 intermediate strains resembled those of benign strains. however, on hoof medium with 2% agar and on blood euonagar, colonies of this virulent and one int ... | 1986 | 2879527 |
| a bacteroides nodosus pili vaccine produced by recombinant dna for the prevention and treatment of foot-rot in sheep. | | 1987 | 2883966 |
| western blot (immunoblot) analysis of the fimbrial antigens of bacteroides nodosus. | the roles of the fimbrial subunit and the putative basal protein antigens in the serological classification of bacteroides nodosus have been examined by western blot (immunoblot)-antibody binding studies of fimbriae isolated from a wide range of strains representative of different serogroups and serotypes. fimbrial subunits were recognized by antiserum against the homologous serogroup but not generally by heterologous antisera, whereas recognition of the basal antigen was independent of serologi ... | 1987 | 2887544 |
| nucleotide sequence of the pilin gene from bacteroides nodosus strain 238 (serogroup g). | | 1987 | 2889185 |
| protection of sheep against footrot with a recombinant dna-based fimbrial vaccine. | recombinant pseudomonas aeruginosa cells containing the bacteroides nodosus fimbrial subunit gene under the transcriptional control of a strong promoter produce large amounts of b. nodosus-type fimbriae. we have carried out vaccination trials which show that these fimbriae are just as effective as either natural fimbriae or whole cell preparations of b. nodosus in inducing protective immunity against homologous footrot challenge. the recombinant-produced fimbriae are also effective therapeutical ... | 1987 | 2891214 |
| efficacy against footrot of a bacteroides nodosus 265 (serogroup h) pilus vaccine expressed in pseudomonas aeruginosa. | pili of bacteroides nodosus 265 (serogroup h) were expressed in pseudomonas aeruginosa both alone and in combination with pili from b. nodosus 198 (serogroup a). pilin genes from b. nodosus were introduced on plasmid-borne, thermoregulated expression systems, either singly or as tandem genes from two separate strains in a single transcription unit. despite the absence of a posttranslational cleavage of pilin which occurs in b. nodosus, pili prepared from p. aeruginosa harboring the pilin gene of ... | 1988 | 2893772 |
| expression of multiple types of n-methyl phe pili in pseudomonas aeruginosa. | the nature of pili synthesized by pseudomonas aeruginosa when plasmid-borne genes of homologous pilins from bacteroides nodosus are introduced as thermoregulated expression systems has been ascertained. expression of b. nodosus pili inhibited the production of indigenous p. aeruginosa pili, and an organism harbouring pilin genes from two strains of b. nodosus produced two serologically distinct populations of pili on each cell. simultaneous production of both indigenous and foreign pili was achi ... | 1987 | 2896292 |
| [an immunological study of human t-cell leukemia virus type i (htlv-i) infection (ii)--radioimmunoprecipitation and sds-polyacrylamide gel electrophoretic analysis of antibody of carriers]. | | 1987 | 2897404 |
| pilins of bacteroides nodosus: molecular basis of serotypic variation and relationships to other bacterial pilins. | | 1988 | 2901028 |
| isolation of a specific mu-opiate receptor peptide, morphiceptin, from an enzymatic digest of milk proteins. | specific radioimmunoassays have been developed for the measurement of naturally occurring morphiceptin and beta-casomorphin. these peptides and related exorphins were isolated from an enzymatic digest of caseins by chromatographic techniques including gel filtration, hydrophobic column and multiple-step high pressure liquid chromatography. three exorphins were purified and characterized in their radioimmunological, biological, and chemical properties. they were identified as morphiceptin, beta-c ... | 1985 | 2991261 |
| felv-induced feline acquired immune deficiency syndrome. a model for human aids. | | 1986 | 3008168 |
| [ecology and epidemiology of transmissible viruses of sandflies in italy]. | | 1986 | 3019208 |
| primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen. | the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ... | 1986 | 3028680 |
| drug-resistant tuberculosis in a southern california hospital. trends from 1969 to 1984. | resistance to one or more antituberculosis drugs was found in 98 of 281 (35%) patients hospitalized at harbor-ucla with culture-positive tuberculosis between 1980 and 1984. resistance to antituberculosis drugs occurred in 23% of patients who had not been previously treated, whereas previously treated patients had a 59% rate of resistance. the overall rates of drug resistance had not significantly changed from prior studies at this hospital. drug resistance was also found in patients with extrapu ... | 1987 | 3109291 |
| an analysis of the organization and evolution of type 4 fimbrial (mephe) subunit proteins. | we have analyzed and compared the amino acid sequences of the type 4 fimbrial subunits from pseudomonas aeruginosa. moraxella bovis, m. nonliquefaciens, bacteroides nodosus, neisseria gonorrhoeae, and n. meningitidis. we propose a consensus sequence for the highly conserved amino-terminal regions of these proteins. in the variable regions, a domain corresponding to an epitope common to n. gonorrhoeae and n. meningitidis fimbriae is conserved, both in sequence and in environment, in fimbrial subu ... | 1987 | 3118043 |
| nucleotide sequence of the pilin gene of bacteroides nodosus 340 (serogroup d) and implications for the relatedness of serogroups. | the gene encoding pilin of bacteroides nodosus 340 has been isolated and the nucleotide sequence determined. the gene is present as a single copy within the b. nodosus genome and a protein of mr 16683 can be predicted from the proposed coding region. a comparison of the predicted amino acid sequence with pilin from other strains of b. nodosus indicated that the protein of strain 340 (serogroup d) has a high degree of similarity with pilin of strain 265 (serogroup h). the degree of similarity bet ... | 1988 | 3183618 |
| antibody responses of sheep vaccinated with foot rot vaccines. | enzyme-linked immunosorbent assay (elisa) and crossed immunoelectrophoresis (iep) were used to investigate antibody responses of sheep vaccinated with a double adjuvanted or single adjuvanted commercial foot rot vaccine. elisa detected an antibody response of greater magnitude to the double adjuvant vaccine compared with the single adjuvant vaccine. sera from sheep vaccinated with double adjuvant vaccine recognised at least six antigens of bacteroides nodosus in crossed iep while sera from the s ... | 1988 | 3222555 |
| [total residue analysis of amitraz in the rabbit tissue by gas chromatography]. | | 1988 | 3243539 |
| onchocerciasis in sierra leone 3: relationships between eye lesions and microfilarial prevalence and intensity. | the relationship between severe onchocercal eye lesions (iritis, sclerosing keratitis, optic atrophy and choroidoretinitis) and (i) the prevalence and intensity of microfilariae (mf) of onchocerca volvulus in skin snips from the iliac crest and outer canthus, and (ii) the prevalence of mf in the cornea and anterior chamber of the eye, was studied in 1414 persons from forest and savanna villages and 312 attenders at eye clinics. ecologically the savanna of sierra leone more closely resembles the ... | 1988 | 3256114 |
| production of mouse monoclonal antibodies for the analysis of idiotypes in serum of patients with chronic lymphatic leukaemia. | in this report a simple procedure for the production of murine monoclonal antibodies (moab) against the idiotype of malignant b cells is described. mice were immunized with lymphoid cells from patients with b-cell chronic lymphocytic leukaemia (b-cll). after fusion of the spleen cells, hybridoma supernatants were screened for anti-idiotypic moab in elisa with immunoglobulins obtained from tumour-cell lysates, xenohybridomas and patients' sera. the anti-idiotypic moab were used to study tumour ce ... | 1988 | 3257881 |
| acute effects of surgery on carbohydrate production and utilization in the fed rat. | 1. the work utilized a model of uncomplicated abdominal surgery (laparotomy under ether anaesthesia) to delineate the effects of abdominal trauma on glucose homoeostasis in the fed rat. 2. regulation of glucose production and utilization was investigated by observing the response to the administration of glucose, insulin plus glucose and 5-methylpyrazole 3-carboxylic acid. 3. glucose administration suppressed hepatic glucose output as assessed by portal-venous concentration differences in contro ... | 1988 | 3276438 |
| the man behind the eponym. paul langerhans. life and work. part iv: publications. | | 1987 | 3307477 |
| [toxicity of prometryne in the lactation period in rats]. | | 1988 | 3369200 |
| genetic-associated resistance to foot rot in selected targhee sheep. | three targhee rams obtained from the ohio agricultural and experimental station had been identified as foot rot resistant on the basis of results of challenge exposure. in the first breeding trial, when rams were bred to 20 foot rot-susceptible ewes, the percentages of foot rot-resistant offspring from the 3 foot rot-resistant rams were 68, 82, and 100, compared with 55 and 60 for foot rot-resistant offspring from 2 known foot rot-susceptible rams. in the second year, the foot rot-resistant rams ... | 1988 | 3372300 |
| [study of the effect of antitumor agents on cell cycle traverse by flow cytometry]. | the effect of antitumor agents on the cell cycle traverse of cultured fl cells, a monolayer culture line derived from human amnion, was investigated by flow cytometry (fcm) in combination with simultaneous determination of total cell number, viability, mitotic index and labeling index by [3h]tdr. the results obtained can be summarized as follows: antimetabolites prolonged the duration of the s phase at a low dose, while at a high dose they decreased the rate of progression from the g1 to s phase ... | 1988 | 3389824 |
| estimating the soluble enzyme activity in brain tissue. | | 1988 | 3416451 |
| diagnosis of congenital toxoplasmosis in ovine foetuses. | | 1987 | 3426476 |
| rapid and fine adjustment of corticosteroidogenesis operating in the adrenal gland of the mongolian gerbil (meriones unguiculatus) superfused in-vitro. | corticosteroid release from adrenals of male adult mongolian gerbils (meriones unguiculatus) has been studied during continuous and discontinuous in-vitro superfusion. corticosteroid output from glands of untreated animals with plasma corticosteroid levels below 300 ng/ml was 3.3 +/- 0.3 ng/adrenal pair/min and decreased only slightly with the length of superfusion (60 min: 3.1 +2- 0.3 ng/adrenal pair/min). in-vitro secretion was significantly higher from adrenals of animals which had corticoste ... | 1987 | 3428359 |
| comparative antigenic analysis of extracellular proteins of bacteroides nodosus isolated from virulent and benign ovine footrot. | antigens in the extracellular protein (ecp) complexes of bacteroides nodosus, isolated from sheep with either benign or virulent footrot, were studied by immunoelectrophoresis (iep). rabbit antisera against ecp from virulent and benign strains, were used in homologous and heterologous crossed iep. four precipitin peaks unique to the virulent strain, and five peaks unique to the benign strain were identified. in an attempt to characterize the different antigens in ecp, rabbit antisera were raised ... | 1986 | 3529606 |
| biochemical and functional characterization of the rat liver glucose-transport system. comparisons with the adipocyte glucose-transport system. | the properties of the glucose-transport systems in rat adipocytes and hepatocytes were compared in cells prepared from the same animals. hormones and other agents which cause a large stimulation of 3-o-methylglucose transport in adipocytes were without acute effect in hepatocytes. hepatocytes displayed a lower affinity for 3-o-methylglucose (20 mm) and alternative substrates than adipocytes (6 mm), whereas inhibitor affinities were similar in both cell types. the concentration and distribution o ... | 1986 | 3548706 |
| macromolecules can pass through occluding junctions of rat ileal epithelium during cholinergic stimulation. | crypt, but not villus, goblet cells in the ileum accelerate their secretion of mucus within 5 min following cholinergic stimulation. this study was done to determine whether the macromolecular permeability and structure of occluding junctions in the ileum are altered during accelerated secretion. rats were injected intravenously with horseradish peroxidase followed by carbachol (250 micrograms/kg, subcutaneous) and the intestinal mucosa was fixed 3-12 min later. in control mucosa (saline-injecte ... | 1987 | 3568101 |
| observations on the binding of lanthanides and calcium to vitamin d-dependent chick intestinal calcium-binding protein. implications regarding calcium-binding protein function. | the binding of calcium and terbium to purified chick vitamin d-dependent intestinal calcium-binding protein was studied by terbium fluorescence, circular dichroism, and intrinsic protein fluorescence techniques. calcium-binding protein bound, with high affinity, at least 3 mol of terbium/mol of protein; numerous low affinity terbium-binding sites were also noted. the three highest affinity sites were resolved into one very high affinity site (site a) and two other sites (sites b and c) with slig ... | 1987 | 3571269 |
| vaccination against lumpy jaw and measurement of antibody response in wallabies (macropus eugenii). | successful protection against lumpy jaw disease in a colony of captive wallabies (macropus eugenii) was induced by vaccination with a commercial ovine footrot vaccine. no mortalities attributable to lumpy jaw were observed in 69 vaccinated animals while six of 42 unvaccinated control wallabies died of the disease. vaccinated animals exhibited significant increases in antibody titres to bacteroides nodosus after the first and second doses of vaccine. titres were measured by an enzyme-linked immun ... | 1987 | 3629891 |
| mechanisms of absorption, transport and tissue uptake of rrr-alpha-tocopherol and d-gamma-tocopherol in the white rat. | the metabolism of alpha- and gamma-tocopherol was studied in three groups of rats that were fed a modified ain-76 diet containing normal (ne, 0.2 g alpha-tocopherol/kg), high (he, 1.0 g alpha-tocopherol/kg) or low (le, less than 0.02 alpha-tocopherol/kg) vitamin e for 3 mo. after 1, 2 and 3 d of an oral dose of 20 mg of alpha-tocopherol, gamma-tocopherol or both, the levels of the two vitamers were measured in plasma and tissues and in some cases in isolated microsomal and mitochondrial fraction ... | 1987 | 3655936 |
| [functional activity of natural killers in viral hepatitis a]. | the direct cytotoxic test with the use of c and neoplastic cells of human myeloleukemia k-562 was employed to examine the activity of natural killers (nk) in 234 patients with viral hepatitis and in 51 healthy subjects as control. it was ascertained that in viral hepatitis, the functional activity of nk was appreciably altered. at the disease onset it tended toward lowering, whereas before the recovery it consistently increased. in cyclic hepatitis, the cytotoxicity rose considerably 2 to 3 week ... | 1986 | 3704941 |
| compulsive attentive behavior after lesion of the ventral striatum in the cat: a behavioral and electrophysiological study. | bilateral lesions of the nucleus accumbens in cat elicited the following changes: preservation in tests requiring focused attention, with difficulty to shift to other targets; paucity of movements, animals displaying moderate hypokinesia and loss of reaction to changes in the environment. these symptoms were accompanied by a significant increase in the amount of beta rhythms, an activity that has been shown to be concomitant with the development of focused attentive behavior. the observed behavi ... | 1986 | 3709743 |
| comparison of treatment methods for the control of contagious ovine foot rot. | four methods of treatment for ovine foot rot were evaluated: foot trimming and topical treatment (brief, daily foot bathing in 10% zinc sulfate); vaccination with an oil-alum adjuvanted, multivalent, bacteroides nodosus bacterin, vaccination with the bacterin plus foot trimming and brief foot bathing, and 1-hour 10% zinc sulfate foot soaks. compared with results of sheep in a nontreated control group, vaccination reduced the number of infected feet by 8%. daily walk-through foot bathing reduced ... | 1986 | 3744977 |
| [a case of staphylococcal scalded skin syndrome in an adult]. | | 1986 | 3807012 |
| [properties of bacteroides nodosus isolated from cattle]. | a total of five strains of bacteroides nodosus were isolated from cattle with interdigital dermatitis on a number of farms. with regard to their cultural and morphologic properties and response to carbohydrates these strains did not differ from b. nodosus strains that caused foot rot in sheep. however, their k-antigen was different from the one of the b. nodosus strains isolated from sheep with foot rot as established via the hemagglutination test. no antigenic variations were found with the str ... | 1986 | 3811203 |
| reactions of murine monoclonal antibodies to blood group mn antigens. | balb/c mice were immunized by intraperitoneal injections with human blood group substances; 4 mice with m and 4 with n. immune spleen cells were fused with murine myeloma cells x63-ag-8.653. two clones secreting monoclonal anti-m and seven secreting monoclonal anti-n were identified. all antibodies were of igg1 subclass and had kappa light chains. three clones have been maintained that produced antibodies useful for typing purposes: anti-m, a09 originating from a mouse injected with m, anti-n, a ... | 1986 | 3811319 |
| reactivities of neoplastic t cells to human autoantibody and monoclonal antibodies. | | 1985 | 3873778 |
| lipid peroxidation-induced inhibition of gamma-aminobutyric acid uptake in rat brain synaptosomes: protection by glucocorticoids. | incubation of rat brain synaptosomes with xanthine and xanthine oxidase (x/xo) resulted in an inhibition of gamma-aminobutyric acid (gaba) uptake. the inhibitory effects of x/xo were temperature- and time-dependent, and were characterized by an increased km for gaba and a decreased vmax. inhibition of gaba uptake by x/xo was associated with both the formation of malonyldialdehyde (mda) and conjugated dienes, indicating that lipid peroxidation was involved. studies with catalase, superoxide dismu ... | 1985 | 3882888 |
| temporal relationships and characterisation of extracellular proteases from benign and virulent strains of bacteroides nodosus as detected in zymogram gels. | extracellular proteases produced by bacteroides nodosus in a peptone rich modified trypticase-arginine-serine broth medium were separated and characterised by relative mobility (rf) in electrophoretic zymogram gels. one benign and two virulent protease banding patterns were established with isolates from sheep, cattle and goats. they correlated with other laboratory tests for virulence but were independent of serogroup. the electrophoretic zymogram method was unable to differentiate intermediate ... | 1985 | 3906802 |
| laminitis and interdigital dermatitis and heel horn erosion. a european perspective. | laminitis is one of the most important claw disorders in dairy herds. nutrition, calving, burdening of the lateral claw of the rear feet, and hereditary susceptibility are all contributing factors. interdigital dermatitis in cattle may be a result of infection by bacteroides nodosus and fusobacterium necrophorum. if this infection becomes chronic, heel horn erosion is its consequence. | 1985 | 3907773 |
| characterization of certain proteinase isoenzymes produced by benign and virulent strains of bacteroides nodosus. | three proteinase isoenzymes from one benign strain of bacteroides nodosus and five proteinase isoenzymes from each of two virulent strains of b. nodosus were purified by horizontal slab polyacrylamide gel electrophoresis. the purified isoenzymes hydrolysed casein, collagen i, collagen iii, elastin, alpha-elastin, fibrinogen, gelatin, haemoglobin and alpha-keratin. the ph optima of all the isoenzymes lay between 7.25 and 9.5, the range of 8.75-9.25 being common to all. the isoenzymes were inhibit ... | 1985 | 3912460 |
| structure and expression of adenovirus type 12 e1b 58k protein in infected and transformed cells: studies using antibodies directed against a synthetic peptide. | we have studied the kinetics of synthesis of the early adenovirus type 12 (ad12) e1b 58k tumor antigen during lytic infection and analysed its half-life, intracellular localization and phosphorylation in infected kb and transformed hamster (ha12/7) cells. our analysis has been based on immunoprecipitations using antibodies directed against a synthetic peptide corresponding to the carboxy-terminal end of the e1b 58k protein. its synthesis was first detectable approximately 8 h after infection and ... | 1985 | 3927602 |
| 14c-labeled substrate catabolism by human diploid fibroblasts derived from infants and adults. | untransformed diploid skin fibroblasts from eight normal adults, aged 24 to 74 years, catabolized several 14c-labeled substrates less effectively than cells from ten normal male infants. 14c-labeled substrate metabolism was quantitated either by measuring the evolution of 14co2 from the 14c-labeled compounds or the incorporation of 14c into cellular protein via transamination of tricarboxylic acid cycle intermediates derived from the 14c-labeled substrates. with these methods, adult cells catabo ... | 1985 | 3936490 |
| immunisation of cattle against interdigital dermatitis (foot-rot) with an autogenous bacteroides nodosus vaccine. | | 1986 | 3964147 |
| genetic control of production of igg antibody against allogeneic erythrocytes in rats. | | 1985 | 3969714 |
| primary structure of pilin protein from bacteroides nodosus strain 216: comparison with the corresponding protein from strain 198. | the amino acid sequence of pilin protein from bacteroides nodosus strain 216 was determined. the protein had a calculated molecular weight of 15962 and contained the same number of amino acid residues (151) as the pilin from the previously sequenced strain 198. the sequence of the first 44 residues was common to both strains, including the unusual amino-terminal amino acid, n-methylphenylalanine. of the remaining 107 residues, 37% of them differed between the two strains. comparison of hydrophil ... | 1985 | 3989505 |
| serogroups of bacteroides nodosus isolated from ovine footrot in britain. | | 1985 | 3992861 |
| failure of oral zinc therapy to alleviate bacteroides nodosus infections in cattle and sheep. | cattle and sheep with bacteroides nodosus infection were treated orally with both high (65 mg zn/kg and 82 mg zn/kg) and low (1 mg zn/kg and 8.6 mg zn/kg) doses of zinc sulphate respectively. the lower dose rates administered weekly for one month, in the case of cattle, or daily for 2 weeks, in the case of sheep, had no effect either on serum zinc levels or the prevalence or severity of infection in treated animals. high dose rates of zinc (approximately 2.5 g zn per head per day) were required ... | 1985 | 4015559 |
| onchocerciasis: experimental models of ocular disease. | onchocerciasis is a leading cause of blindness in equatorial africa and in endemic regions in central america. understanding of the pathologic processes involved in onchocercal eye disease and of the role of immunopathologic mechanisms in its development has been substantially limited by the shortage of eyes for histologic study and by the lack of a naturally occurring animal model. the inoculation of microfilariae of onchocerca species into the eyes of laboratory animals may reproduce selected ... | 1985 | 4070920 |
| inhibition of fusiformis nodosus protease and bovine trypsin by serum alpha macroglobulin. | | 1971 | 4105165 |
| [histoautoradiographic investigation of the dna-synthesis during physiological growth (author's transl)]. | | 1971 | 4130776 |
| rate of thrombin-fibrinogen reaction in some mammalian species. | | 1974 | 4213348 |
| effect of polynucleotides on aminoacyl transfer ribonucleic acid synthetases. 3. inhibition of glutamyl transfer ribonucleic acid synthetase by natural polynucleotides. | | 1968 | 4299520 |
| [comparative histological, histochemical and electron microscopic studies of the lacrimal glands. v. cattle]. | | 1968 | 4301808 |
| dna polymerase activity associated with rna tumor viruses. | c-type rna viruses, originating in mouse, cat, hamster, and viper, catalyze the synthesis of dna from its constituent deoxyribonucleoside triphosphates. both the rate and extent of the reaction were significantly enhanced by brief treatment of the intact virus with ether. a proportion of the newly synthesized dna was associated with virions when intact virus was used as template; this was not the case with ether-treated virus. in both cases, dna extracted from the reaction mixture sedimented slo ... | 1970 | 4318773 |
| studies on radioimmunoassay for arginine vasopressin. | | 1971 | 4334600 |
| lipid composition of quaking mouse myelin: comparison with normal mouse myelin in the adult and during development. | | 1973 | 4350032 |
| lipid composition of quaking mouse myelin: comparison with normal mouse myelin in the adult and during development. | | 1973 | 4350032 |