cultural characteristics and virulence of strains of fusobacterium necrophorum isolated from the feet of cattle and sheep.sixty-one isolates of fusobacterium necrophorum were recovered for study. thirty-one were obtained from lesions of foot abscess in cattle (25) and sheep (6), 28 were from interdigital lesions in cattle and 2 were from the normal interdigital skin of cattle. the majority of isolates from lesions of foot abscess were virulent, belonged to biotype ab (fievez 1963), produced flat, irregular shaped, greyish colonies and haemolysis on blood agar, and grew as turbid filamentous suspensions in liquid me ...19853888165
a microaerophilic coccus in pyogenic infections of ruminants.pyogenic infections of cattle, sheep and goats were examined for the presence of a gram positive bacterium that has been designated "microaerophilic coccus" by other workers. the bacterium was found to be involved in a range of disease processes, including foot and soft tissue abscesses, mastitis, pericarditis and pyometra in cattle, joint and foot abscesses in sheep and foot abscesses in goats. the characteristic feature of the bacterium was its satellitic growth around colonies of other organi ...19853888166
the weak immunogenicity of fusobacterium necrophorum.three of four extreme methods of immunization completely failed to protect mice against challenge with the homologous strain of fusobacterium necrophorum. unsuccessful vaccines included (1) broth culture killed by mild heat and emulsified with freund's complete adjuvant, and (2) a homogenate of heavily infected mouse brains, inactivated by mild heat and given in two doses. also unsuccessful as a method of immunization was the production of a severe subcutaneous infection with f. necrophorum, fol ...19853894509
anaerobes in acute otitis media. 19853897385
repetitive extragenic palindromic (rep) sequences in the escherichia coli gdha gene.deletions of the 3' flanking dna region of the glutamate dehydrogenase (gdh) structural gene from escherichia coli k-12, have been produced on a plasmid that carries the complete gdha gene. those deletions include part of the repetitive extragenic palindromic (rep) sequences proposed by stern et al. [cell 37 (1984) 1015-1026], as a novel and major feature of the bacterial genome. the effect of these deletions on the final gdh level in the cell, has been determined. a broader compilation, analysi ...19853902576
adherence of fusobacterium necrophorum to vero cells.the adherence of the anaerobic species fusobacterium necrophorum to the surface of vero cells was studied. adherence between the bacterium and the tissue culture cells was paralleled by the hemagglutinability of f. necrophorum. treatment of the bacterial cells with lactoalbumin hydrolysate or anti-f. necrophorum-hemagglutinin serum reduced the intensity of the attachment. the purified hemagglutinin bound to the membranes of vero cells. it lost its adherent property when mixed with the homologous ...19853904278
laminitis and interdigital dermatitis and heel horn erosion. a european perspective.laminitis is one of the most important claw disorders in dairy herds. nutrition, calving, burdening of the lateral claw of the rear feet, and hereditary susceptibility are all contributing factors. interdigital dermatitis in cattle may be a result of infection by bacteroides nodosus and fusobacterium necrophorum. if this infection becomes chronic, heel horn erosion is its consequence.19853907773
the sfia11 mutation prevents filamentation in a response to cell wall damage only in a reca+ genetic background.n-alpha-palmitoyl-l-lysyl-l-lysine dihydrochloride ethyl ester (pll) at sublethal doses causes filamentous growth of e. coli strains except sfia mutants, which divide normally in its presence. pll does not elicit the sos responses as judged by lambda prophage induction, an increase of reca protein synthesis or induction of the sfia operon in a sfia::lacz fusion strain. thus, it appears that filamentation caused by pll is not an sos function and might be the result of membrane damage by pll, whic ...19853911030
identification of common antigens in ribosome-rich extracts from fusobacterium necrophorum.ribosome-rich extracts (rre) were prepared by differential and ultracentrifugation from 25 bovine and 6 ovine isolates of fusobacterium necrophorum (fn) including both biotypes a and b. a pooled rabbit antiserum was prepared against whole-cell and sonicated whole-cell bacterins of f necrophorum isolate fn 3080, and a 2nd pooled rabbit antiserum was prepared against a rre of fn 3080. the rre of the 25 bovine isolates were tested against the fn 3080 whole-cell antiserum, using ouchterlony double-i ...19853918487
synthesis and pharmacological properties of n,n'-bis-acylderivatives of phenobarbital. 19863956715
in vitro production of anti-influenza virus antibody after simultaneous administration of h3n2 and h1n1 cold-adapted vaccines in seronegative children.sixteen doubly seronegative (h3n2 and h1n1) young children were recently enrolled in a study of live, attenuated cold-adapted influenza a vaccines. twelve children received simultaneously h3n2 and h1n1 live, attenuated influenza vaccine intranasally and four received saline as placebo controls. peripheral blood lymphocytes (pbl) were obtained sequentially from all children and the in vitro production of anti-influenza h3n2 and h1n1 antibody from unstimulated and influenza stimulated cells was me ...19863962451
comparative changes in a rat liver abscess model induced with three fusobacterium necrophorum strains.viable cultures of 3 bovine fusobacterium necrophorum strains were inoculated into the hepatic portal veins of rats. the experimentally produced hepatic necrobacillosis lesions were examined beginning at 15 minutes after inoculation was done and continuing for 4 weeks. fusobacterium necrophorum 2101 (fn 2101), a biotype a, was the most virulent; f necrophorum 2030 (fn 2030), a biotype ab, was of intermediate virulence; and f necrophorum 2035 (fn 2035), a biotype b, was the least virulent. gross ...19863963598
development of enzyme-linked immunosorbent assays for the detection of fusobacterium necrophorum antibody in animal sera.enzyme-linked immunosorbent assays (elisa) for the detection of fusobacterium necrophorum antibody in the sera of rabbits, cattle, and sheep were developed, using a ribosome-rich extract (rre) from f necrophorum as the antigen. test character, including optimal antigen dilution and substrate incubation periods, was established, using rabbit, bovine, and ovine antisera produced against rre from isolates of f necrophorum. rabbit antisera produced against 7 other species of bacteria were used to te ...19853970414
dissecting retropharyngeal abscess due to fusobacterium necrophorum in an adult.i have described an adult patient who had dissecting retropharyngeal abscess complicated by pneumonia, mediastinitis, pericarditis, sepsis, and status epilepticus.19853983669
satranidazole: experimental evaluation of activity against anaerobic bacteria in vitro and in animal models of anaerobic infection.satranidazole, a 5-nitroimidazole derivative, was tested for its activity against reference strains and clinical isolates of anaerobic bacteria in vitro and in two murine models of anaerobic infection in comparison with metronidazole, tinidazole, ornidazole and clindamycin. the mic90 of satranidazole against 50 clinical isolates of anaerobes was 0.25 mg/l which was four-fold lower than the mic90 of metronidazole, tinidazole and ornidazole (mic90 = 1.0 mg/l). in a fatal murine infection with fuso ...19854008378
plasmid in fusobacterium necrophorum. 19854010130
cyclopentenyl cytidine analogue. an inhibitor of cytidine triphosphate synthesis in human colon carcinoma cells.the mechanism of action of the cyclopentenyl analogue of cytidine, ccyd, was investigated in human colon carcinoma cell line ht-29. upon exposure of cells to 10(-6)m ccyd, cell viability was reduced to 20% of control, whereas cytocidal activity was not present after 2 hr of drug exposure. cell lethality was partially reversible by urd, cyd or dcyd at 10(-6)m ccyd, and fully reversible by these nucleosides at 2.5 x 10(-7)m ccyd. the incorporation of [14c]dthd and [3h]urd into dna and rna was inhi ...19854015694
fecal mutagenicity arising from ingestion of fried ground beef in the human.fried ground beef has been shown to contain mutagens, and the major mutagenic component has been identified as 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx). mutagens in feces of 3 adult volunteers were fractionated by treatment of the feces with blue cotton followed by chromatography on a carboxymethyl cellulose column. the chromatographic fraction, corresponding to meiqx in terms of the position of elution, was examined for mutagenicity in s. typhimurium ta98 with metabolic activation. ...19854022023
technique for percutaneous pulmonary arterial catheterization in conscious dogs.a technique was developed to allow percutaneous placement and exchange of pulmonary arterial catheters in conscious dogs. seven dogs were catheterized weekly for 5 weeks, using a 10-gauge over-the-needle cannula. a no. 7 f flow-directed catheter was passed through the cannula to measure right heart and pulmonary arterial pressures and to calculate cardiac output. the flow-directed catheter was removed and replaced with an arteriographic catheter, and a pulmonary arteriogram was performed. placem ...19854026036
aggregation of platelets by fusobacterium necrophorum.broth cultures and washed cells of 13 of 24 bovine isolates of fusobacterium necrophorum aggregated human platelets in platelet-rich plasma. the cell-free culture fluid was inactive. bacteria stored at 4 degrees c in saline remained active for at least 3 months, but they did not release activity into the storage solution. aggregation typically began within 1 min after the addition of 10(3) bacteria to 10(3) platelets was complete within 5.5 min. assays for cytosolic lactic dehydrogenase revealed ...19854031037
elevation in blood sialic acid and mucoprotein levels during hepatic abscess in cattle. 19854032925
isolation of a bacteriophage in fusobacterium necrophorum. 19854032936
antitumour activity of some platinum compounds.a group of cis-ethylenediammine platinum (ii) complexes were synthesized and their activity against sarcoma-180 ascites tumour cells in mouse was tested. one of the compounds, pt(hoch2ch2nhch2ch2nh2)cl2, showed significant antitumour activity having little toxicity to the host. like the parent compound, cis-ddp, it binds to dna, but transcription is not the primary process inhibited by these compounds. the drug-dna complex, though less toxic, was not more effective than the free drug.19854038640
congenital cataracts in a free-ranging rhesus monkey.this report documents the occurrence of congenital cataracts in a visually-impaired yearling rhesus monkey from the same matriline on cayo santiago in which a congenitally blind infant was born 2 years previously.19854046005
effects of the components of fusobacterium necrophorum in experimental liver abscess formation in mice. 19854046264
chemical and biological properties of lipopolysaccharides from fusobacterium necrophorum biovar a and biovar b strains. 19854046268
fusobacterium necrophorum septicemia without a primary focus.we have presented a case of fusobacterium necrophorum septicemia, an uncommon infection whose early course may be typified by vague diffuse symptoms or a seemingly benign upper respiratory tract inflammation mimicking acute viral syndromes or viral pharyngitis. the septicemia is most commonly, but not always preceded by an obvious oropharyngeal focus.19854071187
determination of urinary lysozyme during aminoglycoside therapy--appraisal of lysozyme inhibition caused by gentamicin and tobramycin. 19854075521
sphaerophorus necrophorus. a study of 23 strains. 19714109880
a satisfactory medium and technique for the mass cultivation of sphaerophorus necrophorus. 19734121315
uses of banding techniques for the identification of human diseases of cytogenetic origin. 19734131094
striatal tyrosine hydroxylases with high and low affinity for tyrosine: implications for the multiple-pool concept of catecholamines. 19734148015
structural studies of human immunoglobulins. differences in the primary structure of heavy chains of normal and pathologic g, a, m immunoglobulins. 19654159529
[comparison of media with antibacterial preparations in the bacteriological diagnosis of dysentery]. 19684184658
serological investigations on endotoxins isolated from sphaerophorus funduliformis (syn. fusobacterium necrophorum) strains. 19744217645
comparative immunochemical studies on the endotoxins of two fusobacterium necrophorum strains. 19744217646
kinetic studies on a brain microsomal adenosine triphosphatase. ii. potassium-dependent phosphatase activity. 19694241304
isolation of sphaerophorus necrophorus from bovine hepatic abscesses in british columbia.the purpose of this study was to determine the predominant microflora in hepatic abscesses of cattle slaughtered in british columbia. samples of approximately 400 livers were examined by direct smear and culture. sphaerophorus necrophorus was present in 97% of the 431 abscesses and in 67% it was present in pure culture. in 30% it was present in combination with other organisms such as coryne-bacterium and streptococcus.19714253458
action of an n-acetylmuramidase from a limax amoeba on the murein of proteus mirabilis. 19714256271
[comparative study of the convulsion provoking activity of actinomycin d and methionine sulfoximine injected into the cerebrospinal fluid of dogs]. 19714262072
cultivation and maintenance of sphaerophorus necrophorus. i. an anaerobic medium for aerobic incubation.out of 185 media formulated and studied for the growth of sphaerophorus necrophorus, ten were selected for intensive investigation. their ability to promote growth was compared photometrically. medium 156 made up of brewer's thioglycollate broth, yeast extract, l-cystine, and ascorbic acid, was found to yield a very heavy growth. the viability of s. necrophorus was maintained indefinitely by weekly subcultures. when not subcultured, all isolates remained viable for fifteen weeks and some isolate ...19744272964
results of a preliminary trial with sphaerophorus necrophorus toxoids to control liver abscesses in feedlot cattle.a preliminary field experiment was undertaken to evaluate the efficacy of alum precipitated toxoids of sphaerophorus necrophorus prepared from sonicated whole cells and cell fractions to reduce the incidence of bovine abscesses. a total of 108 calves were divided into five groups and treated as follows: i. uninoculated control, ii. adjuvant inoculated control, iii. 15.5 mg protein of sonicated (fragmented cells) toxoid, iv. 10.5 mg protein of cytoplasmic toxoid. v. 15.5 mg protein of cytoplasmic ...19744277634
membrane-bound adenosinetriphosphatase of escherichia coli. ii. physicochemical properties of the enzyme. 19744281778
antibiotic susceptibility patterns as aids in classification and characterization of gram-negative anaerobic bacilli.patterns of susceptibility of gram-negative anaerobic bacilli to antibiotics have been found to be distinctive and of significant help in classification and identification. five major groups of gram-negative anaerobic bacilli have been defined on the basis of morphological and biochemical criteria. antibiotic sensitivity patterns conform to these groupings and provide additional taxonomic criteria. the bacteroides fragilis group is resistant to penicillin g, whereas the other groups are generall ...19674293666
moss-ill--a possible hypocalcaemia of hill ewes. 19694306077
depression of centrum medianum complex response by rostral thalamic lesion. 19704313185
[empyema of the pleura caused by the anerobe, non-spore-producing bacteria species sphaerophorus necrophorus]. 19714323168
18-hydroxy-deoxycorticosterone in human hypertension. 19714327960
tumor production in squirrel monkeys (saimiri sciureus) by rous sarcoma virus. 19714329233
[the replicative complexes of sendai virus]. 19714332921
host defense mechanisms against herpes simplex virus. i. control of infection in vitro by senstized spleen cells and antibody.sensitized mouse spleen cells decrease the spread of herpes simplex virus infection in cell culture lines derived from human and murine tissues. these washed, sensitized cells act alone and additively in combination with antibody to diminish the ability of single virus-infected cells to spread infection to contiguous cells. this control of infection is not species specific, unlike interferon, and appears to be distinct from the effect of antibody. lymphotoxin was not detected in this lymphocyte- ...19734351970
activity of foot-and-mouth disease virus rna-dependent rna polymerase in vitro: inhibition by polyamines and poly(amino acid)s. 19734354232
activity of foot-and-mouth disease virus rna-dependent rna polymerase in vitro: inhibition by polyamines and poly(amino acid)s. 19734354232
corneal collagenases: evidence for zinc metalloenzymes. 19734356811
metabolic regulation of steroidogenesis in adrenocortical carcinoma cells of rat. effect of adrenocorticotropin and adenosine cyclic 3',5'-monophosphate on the incorporation of (20s)-20-hydroxy(7 alpha-3h)cholesterol into deoxycorticosterone and corticosterone. 19744362943
immunopathogenicity and oncogenicity of murine leukemia viruses. i. induction of immunologic disease and lymphoma in (balb-c times nzb)f1 mice by scripps leukemia virus.this report clearly demonstrates that a systemic lupus erythematosus (sle)-like syndrome and lymphoma can be induced in immunologically normal (balb/c x nzb)f(1) mice by infection of neonates with a murine leukemia virus (mulv) (scripps leukemia virus [slv] 60a) isolated from nzb lymphoblasts. slv 60a was titered in vitro (xc test) and administered to newborn and adult (balb/c x nzb)f(1) mice over six log(10) dilutions. propagation of mulv in the newborn recipients was indicated by greatly eleva ...19744372290
[lung infiltrates, degeneration of muscles in the extremities and development of hypertension in a young man]. 19714400782
letter: more about vitamin c. 19744431377
isolation of sphaerophorus necrophorus from bovine liver abscesses in the sudan. 19744460324
[the induction of liver cirrhosis in rats by simultaneous administration of carbon tetrachloride and phenobarbitone (author's transl)]. 19734518152
polysaccharide accumulation in fusiformis necrophorus. 19744530608
a selective medium for the isolation of sphaerophorus necrophorus. 19724562968
fluorescent antibody technique for identifying isolates of sphaerophorus necrophorus of bovine hepatic abscess origin. 19724562969
enhancement of asparaginase-induced regression of the 6c3hed lymphosarcoma by protein-free diet. 19734574058
strain specific immunity to plasmodium berghei: a new genetic marker. 19734579579
bacteroidaceae in thromboembolic disease: effects of cell wall components on blood coagulation in vivo and in vitro.the effects of bacteroides sp., fusobacterium mortiferum, bacteroides fragilis, and sphaerophorus necrophorus on various parameters of blood coagulation in vivo and in vitro were determined and compared to the coagulation effects of escherichia coli and salmonella minnesota, wild type and r595. intravenous injection of washed cells, culture filtrate, lipopolysaccharide, or lipid a of the anaerobic gram-negative microorganisms into mice resulted in acceleration of coagulation. lipopolysaccharide ...19734594118
a serological study of strains belonging to sphaerophorus necrophorus, sph. varius, and sph. freundii. 19724628422
streptococcal pharyngeal infection carrier rates in schoolchildren in shiraz. 19724636101
preliminary characterization of the 5 -dihydrotestosterone binding protein in the epididymal cytosol fraction. in vivo studies. 19724678201
interaction of liver plasma membranes and gtp with gtp hydrolysis. 19734712199
replication of human endothelial cells in culture.investigative studies dealing with the properties and functions of endothelial cells have been hampered because there has been little or no success in the isolation, growth, and passage of individual cells in large numbers. we have developed a system whereby pure cultures of endothelial cells derived from umbilical veins can be subcultured for at least five serial passages. many facets of endothelial function and interaction can be evaluated with the use of this new adaptive system of isolation ...19734718112
amino acid and peptide requirement of fusiformis necrophorus.uptake of individual amino acids and peptides by fusiformis necrophorus was studied in growing cultures and resting cell suspensions. the cells were able to incorporate 16 of 17 (14)c-labeled amino acids into cell protein, the exception being proline. proline could neither be formed by the cells from any of the other tested amino acids nor be synthesized from glucose or serine when these were used as energy sources. the addition of di- and tripeptides, the octapeptides vasopressin and oxytocin, ...19734745417
assessment of various adjuvants in sphaerophorus necrophorus toxoids. 19734756812
the toxicity of the molluscicides metaldehyde and methiocarb to dogs. 19734772564
changes in serum gonadotrophin levels, and in protein levels and in-vivo incorporation of (35s)methionine into protein of discrete brain areas and the anterior pituitary of the rat during the oestrous cycle. 19734795507
[total synthesis of 19-nortestosterone analogues (author's transl)]. 19744859176
the pathogenic synergy of fusiformis necrophorus and corynebacterium pyogenes. i. influence of the leucocidal exotoxin of f. necrophorus. 19674865628
the pathogenic synergy of fusiformis necrophorus and corynebacterium pyogenes. ii. the response of f. necrophorus to a filterable product of c. pyogenes. 19674865629
infective bulbar necrosis (heel-abscess) of sheep, a mixed infection with fusiformis necrophorus and corynebacterium pyogenes. 19684870831
the lac operator is dna. 19674873589
the lac operator is dna. 19674873589
experimental suppression of allograft rejection: background and application. 19684873843
experimental suppression of allograft rejection: background and application. 19684873843
[sensitivity to radiomimetics and chemical mutagens of escherichia coli strains of group k 12, possessing different abilities to repair radiation damage]. 19674876361
growth of bacteroidaceae in stirred fermentors.the conditions for increasing bacterial yields in cultures of bacteroidaceae by the use of stirred fermentors and ph control were investigated by means of three representative species: sphaerophorus necrophorus, bacteroides fragilis, and b. melaninogenicus. a medium containing tryptone, yeast extract, and glucose or sucrose was used. horse serum had to be added to obtain substantial growth of b. melaninogenicus. the optimal ph for growth rate and yield was 7.0 to 7.2. lysis of the bacteria occur ...19694896882
magnesium deficiency and distribution of blood in the rat. 19704908844
effect of bile and desoxycholate on gram-negative anaerobic bacteria.the bile tests for characterizing gram-negative anaerobic bacilli were reevaluated in prereduced anaerobically sterilized peptone-yeast-glucose broth, in thioglycollate broth, and on blood agar plates. blood agar plates were unsatisfactory. the combination of 20% bile with 0.1% desoxycholate inhibited fusobacterium, bacteroides melaninogenicus, and b. oralis and sometimes sphaerophorus necrophorus, but not b. fragilis or other sphaerophorus species studied. ten per cent bile with 0.05% desoxycho ...19704922082
application of immunofluorescence to studies on the ecology of sphaerophorus necrophorus.sphaerophorus necrophorus (bovine liver abscess isolates) antiserum was fractionated and labeled with fluorescein isothiocyanate. the fluorescent-antibody (fa) conjugate proved to be species-specific and facilitated the detection of s. necrophorus cells in liver abscesses, viscera, and ruminal contents of cattle. brightly fluorescing s. necrophorus cells were observed in experimentally inoculated soil incubated anaerobically at 37 and 4 c for 8 and 10 months, respectively. when incubated under m ...19714930035
formation of proteolytic activity in continuous culture of sphaerophorus necrophorus. 19714933449
streptomyces sp. producing litmocidin. 19714933936
[observations concerning 7 strains of saphaerophorus necrophorus, sphaerophorus funduliformis, and sphaerophorus pseudonecrophorus species]. 19714939845
genetic complementation between escherichia coli rna polymerase mutants. 19714946184
obesity following disturbance of the ventromedial hypothalamus: a comparison of lesions, lateral cuts, and anterior cuts. 19714948242
the effect of high-energy proton irradiation on the cardiovascular system of the rhesus monkey. 19664957419
[fundamentals of congenital metabolic anomaly--with special reference to experimental phenylketonuria]. 19674966221
preparation and chemical characteristics of endotoxic lipopolysaccharide from three strains of sphaerophorus necrophorus. 19714997133
a circular dichroism study of microtubule protein. 19725028102
trichostrongylus colubriformis: age resistance in the rabbit, oryctolagus cuniculus. 19725057030
effect of amphetamine and chlorpromazine on maternal behaviour of rats. 19725066096
[blood pressure telemetry in man using the microcatheter method]. 19725081732
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