| in vitro growth characteristics and volatile sulfur compound production of solobacterium moorei. | solobacterium moorei has recently been implicated as a causative agent of halitosis. in vitro experiments to evaluate the role of s. moorei in halitosis have, however, been complicated by a paucity of information on the ideal conditions for culturing this organism. this work aimed to optimize a liquid culture medium for s. moorei, and to determine the growth-curve of the organism. further, the ability of s. moorei to generate volatile sulfur compounds was investigated and compared quantitatively ... | 2014 | 24487184 |
| prevotella intermedia induces severe bacteremic pneumococcal pneumonia in mice with upregulated platelet-activating factor receptor expression. | streptococcus pneumoniae is the leading cause of respiratory infection worldwide. although oral hygiene has been considered a risk factor for developing pneumonia, the relationship between oral bacteria and pneumococcal infection is unknown. in this study, we examined the synergic effects of prevotella intermedia, a major periodontopathic bacterium, on pneumococcal pneumonia. the synergic effects of the supernatant of p. intermedia (pisup) on pneumococcal pneumonia were investigated in mice, and ... | 2014 | 24478074 |
| meningitis due to fusobacterium nucleatum after a kart crash. | | 2013 | 24471173 |
| fusobacterium spondylodiscitis: case report and literature review. | fusobacteria are obligate anaerobic bacilli residing in the oral cavity, female genital tract, and intestine. these pathogens are typical components of head, neck, and abdominal abscesses due to contiguous spread from adjacent mucosal surfaces. they are unusual etiologies, however, of bone and joint infections, particularly outside the cranial region. we report an unusual case of hematogenous lumbar spondylodiscitis caused by fusobacterium nucleatum of suspected odontogenic origin. | 2014 | 24462349 |
| preservation of bacterial dna by human dentin. | the capacity of dentin and collagen to bind dna and protect against spontaneous and nuclease-induced degradation was evaluated individually and by the incubation of dna with nuclease-producing bacteria in a mixed culture. | 2014 | 24461411 |
| draft genome sequences of fusobacterium nucleatum chdc f145, chdc f174, chdc f206, and chdc f300, isolated from human subgingival plaques in the republic of korea. | recently, five strains were isolated from human subgingival plaques and were proposed as a novel subspecies of fusobacterium nucleatum. here, we report the draft genome sequences of the strains, except one for which the draft sequence was already introduced. | 2014 | 24459284 |
| in vitro activity of carvacrol against titanium-adherent oral biofilms and planktonic cultures. | the aim of this study was to test the effect of carvacrol against oral pathogens and their preformed biofilms on titanium disc surface. | 2014 | 24458367 |
| radiography-based score indicative for the pathogenicity of bacteria in odontogenic infections. | to develop a new radiography-based score to assess the potential of bacteria to cause odontogenic infections derived from the occurrence of bacteria at small or large radiographical lesions. | 2014 | 24444257 |
| detection of fusobacterium nucleatum and fada adhesin gene in patients with orthodontic gingivitis and non-orthodontic periodontal inflammation. | fusobacterium nucleatum is one of the most abundant gram-negative bacilli colonizing the subgingival plaque and closely associated with periodontal disease. however it is unclear whether f. nucleatum is involved in gingival inflammation under orthodontic appliance. a novel adhesin, fada, which is unique to oral fusobacteria, is required for f. nucleatum binding and invasion to epithelial cells and thus may play an important role in colonization of fusobacterium in the host. in this study, we eva ... | 2014 | 24416378 |
| antibacterial and antigelatinolytic effects of satureja hortensis l. essential oil on epithelial cells exposed to fusobacterium nucleatum. | the present report examined the effects of essential oils (eos) from satureja hortensis l. and salvia fruticosa m. on the viability and outer membrane permeability of the periodontopathogen fusobacterium nucleatum, a key bacteria in oral biofilms, as well as the inhibition of matrix metalloproteinase (mmp-2 and mmp-9) activities in epithelial cells exposed to such bacteria. membrane permeability was tested by measuring the n-phenyl-1-naphthylamine uptake and bacterial viability by using the comm ... | 2015 | 24404975 |
| the effects of a mineral trioxide aggregate-based sealer on the production of reactive oxygen species, nitrogen species and cytokines by two macrophage subtypes. | to test the effects of a mineral trioxide aggregate-based sealer (mta fillapex(®)) and mta (mta-ângelus(®)) on viability and on the production of cytokines, reactive oxygen species (ros) and nitrogen species (no) by m1 and m2 inflammatory macrophages. | 2014 | 24354338 |
| draft genome sequence of fusobacterium nucleatum subsp. animalis chdc f324, isolated from a human subgingival plaque in the republic of korea. | five subspecies of fusobacterium nucleatum have been classified: animalis, nucleatum, polymorphum, vincentii, and fusiforme. f. nucleatum subsp. animalis chdc f324 (kcom 1325) was isolated from a human subgingival plaque in the republic of korea. here, we report the draft genome sequence of the strain. | 2013 | 24336380 |
| draft genome sequence of fusobacterium nucleatum subsp. nucleatum chdc f316, isolated from a human peri-implantitis lesion in the republic of korea. | fusobacterium nucleatum is a gram-negative anaerobe and is one of the causative agents of periodontal diseases, including peri-implantitis. fusobacterium nucleatum subsp. nucleatum chdc f316 (kcom 1322) was isolated from a human peri-implantitis lesion. here, we report the draft genome sequence of this strain. | 2013 | 24336379 |
| draft genome sequence of fusobacterium nucleatum subsp. vincentii chdc f8, isolated from a human subgingival plaque in the republic of korea. | fusobacterium nucleatum is a gram-negative, nonmotile, obligately anaerobic rod bacterium which might play an important role in the initiation and progression of periodontal diseases. f. nucleatum subsp. vincentii chdc f8 (kcom 1231) was isolated from a human gingivitis lesion. here, we report the draft genome sequence of the strain. | 2013 | 24336378 |
| fusobacterium nucleatum activates the immune response through retinoic acid-inducible gene i. | retinoic acid-inducible gene i (rig-i) is a cytosolic pattern recognition receptor involved in the sensing of rna viruses and the initiation of antiviral responses. fusobacterium nucleatum, a gram-negative anaerobic bacterium associated with periodontal disease, is capable of invading cells. we hypothesized that f. nucleatum's ability to invade cells allows the microorganism to activate the immune response through rig-i. bacterial invasion was found to be necessary for f. nucleatum-induced nucle ... | 2014 | 24334410 |
| cetylpyridinium chloride suppresses gene expression associated with halitosis. | halitosis is a common complaint affecting the majority of the population. mouthrinses containing cetylpyridinium chloride (cpc) have been used as oral hygiene aids to suppress oral malodor. although the clinical efficacy of these mouthrinses has been well-documented, the mechanism whereby cpc reduces malodor is less-well-understood. we hypothesized that cpc suppresses expression of the genes (mgl and cdl) and enzymes responsible for methyl mercaptan (ch3sh) and hydrogen sulfide (h2s) production ... | 2013 | 24112735 |
| differential effect of autoinducer 2 of fusobacterium nucleatum on oral streptococci. | autoinducer 2 (ai-2) is a quorum sensing molecule and plays an important role in dental biofilm formation, mediating interspecies communication and virulence expression of oral bacteria. fusobacterium nucleatum connects early colonizing commensals and late colonizing periodontopathogens. f. nucleatum ai-2 and quorum sensing inhibitors (qsis) can manipulate dental biofilm formation. in this study, we evaluated the effect of f. nucleatum ai-2 and qsis on biofilm formation of streptococcus gordonii ... | 2013 | 24112724 |
| fusobacterium nucleatum induces cytokine production through toll-like-receptor-independent mechanism. | to determine whether fusobacterium nucleatum's ability to invade cells allows the bacteria to activate pro-inflammatory response through cytosolic pattern recognition receptors, independent of surface toll-like receptors (tlrs). | 2014 | 24102075 |
| myocardial infection due to fusobacterium nucleatum. | fusobacterium nucleatum is an anaerobic gram-negative bacillus, which inhabits the oropharynx, gastrointestinal tract, and female genital tract. infections classically affect the head and neck. we report a patient with a myocardial mass due to f. nucleatum, initially thought to be a neoplasm, and discuss anaerobic cardiac infections. | 2013 | 24094834 |
| a chlorhexidine-loaded biodegradable cellulosic device for periodontal pockets treatment. | absorbent points widely used in endodontic therapy were transformed into bioresorbable chlorhexidine delivery systems for the treatment of the periodontal pocket by preventing its recolonization by the subgingival microflora. these paper points (pps) were first oxidized to promote their resorption, then grafted with β-cyclodextrin (cd) or maltodextrin (md) in order to achieve sustained delivery of chlorhexidine. we investigated the oxidation step parameters through the time of reaction and the n ... | 2014 | 24090988 |
| progression of periodontal inflammation in adolescents is associated with increased number of porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis, and fusobacterium nucleatum. | the study aims to evaluate the change of related subgingival periodontopathogens among different stage of gingivitis in adolescent and assess the relationship between periodontopathogens and the progression of periodontal inflammation. | 2014 | 24025042 |
| fusobacterium nucleatum potentiates intestinal tumorigenesis and modulates the tumor-immune microenvironment. | increasing evidence links the gut microbiota with colorectal cancer. metagenomic analyses indicate that symbiotic fusobacterium spp. are associated with human colorectal carcinoma, but whether this is an indirect or causal link remains unclear. we find that fusobacterium spp. are enriched in human colonic adenomas relative to surrounding tissues and in stool samples from colorectal adenoma and carcinoma patients compared to healthy subjects. additionally, in the apc(min/+) mouse model of intesti ... | 2013 | 23954159 |
| fusobacterium nucleatum promotes colorectal carcinogenesis by modulating e-cadherin/β-catenin signaling via its fada adhesin. | fusobacterium nucleatum (fn) has been associated with colorectal cancer (crc), but causality and underlying mechanisms remain to be established. we demonstrate that fn adheres to, invades, and induces oncogenic and inflammatory responses to stimulate growth of crc cells through its unique fada adhesin. fada binds to e-cadherin, activates β-catenin signaling, and differentially regulates the inflammatory and oncogenic responses. the fada-binding site on e-cadherin is mapped to an 11-amino-acid re ... | 2013 | 23954158 |
| genotype is an important determinant factor of host susceptibility to periodontitis in the collaborative cross and inbred mouse populations. | periodontal infection (periodontitis) is a chronic inflammatory disease, which results in the breakdown of the supporting tissues of the teeth. previous epidemiological studies have suggested that resistance to chronic periodontitis is controlled to some extent by genetic factors of the host. the aim of this study was to determine the phenotypic response of inbred and collaborative cross (cc) mouse populations to periodontal bacterial challenge, using an experimental periodontitis model. in this ... | 2013 | 23937452 |
| oral fusobacterium nucleatum subsp. polymorphum binds to human salivary α-amylase. | fusobacterium nucleatum acts as an intermediate between early and late colonizers in the oral cavity. in this study, we showed that f. nucleatum subsp. polymorphum can bind to a salivary component with a molecular weight of approximately 110 kda and identified the protein and another major factor of 55 kda, as salivary α-amylase by time-of-flight mass spectrometry and immuno-reactions. salivary α-amylase is present in both monomeric and dimeric forms and we found that formation of the dimer depe ... | 2013 | 23906425 |
| a periodontal pathogen treponema denticola hijacks the fusobacterium nucleatum-driven host response. | periodontitis is a polymicrobial disease that arises from the dysbiosis of the plaque biofilm. to study polymicrobial interactions with gingival epithelial cells, the oral commensal fusobacterium nucleatum and the periodontal pathogen treponema denticola were chosen due to their opposing effects on the expression of human beta-defensins (hbds) and interleukin (il)-8 in gingival epithelial cells. immortalized gingival epithelial hok-16b cells were infected with either f. nucleatum or t. denticola ... | 2013 | 23897119 |
| endobronchial lesion due to pulmonary fusobacterium nucleatum infection in a child. | clinically significant infections due to the members of the genus fusobacterium are rare. the clinical manifestations of pulmonary fusobacterium nucleatum infections range from simple aspiration pneumonia to severe diseases as necrotizing pneumonia, lung abscess, and empyema. endobronchial lesions and obstructions are rarely seen in children and are often a misdiagnosed result in delay of definitive treatment. here, we report a case of endobronchial lesion due to pulmonary f. nucleatum infection ... | 2014 | 23868895 |
| halitosis vaccines targeting foma, a biofilm-bridging protein of fusobacteria nucleatum. | halitosis (bad breath) is estimated to influence more than half of the world's population with varying degree of intensity. more than 85% of halitosis originates from oral bacterial infections. foul-smelling breath mainly results from bacterial production of volatile sulfur compounds (vscs) such as hydrogen sulfide and methyl mercaptan. to date, major treatments for elimination of oral malodor include periodontal therapy combined with antibiotics or antimicrobial agents, and mechanical approache ... | 2013 | 23865430 |
| effects of c-reactive protein on the neutrophil respiratory burst in vitro. | this study determined the influence of physiologically relevant concentrations of c-reactive protein (crp) on reactive oxygen species (ros) production by neutrophils. neutrophils from healthy individuals were incubated with soluble pentameric crp prior to tlr stimulation with fusobacterium nucleatum, or fcγr stimulation with igg-opsonised staphylococcus aureus or heat-aggregated igg. ros generation by unstimulated cells and those after stimulation were determined using luminol, isoluminol and lu ... | 2014 | 23839528 |
| evaluation of the host response in various models of induced periodontal disease in mice. | the aim of this study is to characterize and evaluate the host response caused by three different models of experimental periodontitis in mice. | 2014 | 23805811 |
| interactions between lactobacillus crispatus and bacterial vaginosis (bv)-associated bacterial species in initial attachment and biofilm formation. | certain anaerobic bacterial species tend to predominate the vaginal flora during bacterial vaginosis (bv), with gardnerella vaginalis being the most common. however, the exact role of g. vaginalis in bv has not yet been determined. the main goal of this study was to test the hypothesis that g. vaginalis is an early colonizer, paving the way for intermediate (e.g., fusobacterium nucleatum) and late colonizers (e.g., prevotella bivia). theoretically, in order to function as an early colonizer, spe ... | 2013 | 23739678 |
| necrotizing pneumonia in children: report of 41 cases between 2006 and 2011 in a french tertiary care center. | forty-one children hospitalized for necrotizing pneumonia were retrospectively analyzed. necrotizing pneumonia represented 0.8% of community-acquired pneumonia and 6% of hospitalized community-acquired pneumonia. the chest radiograph revealed necrosis on admission in onethird of cases. twenty-one cases (51%) were documented, including 13 staphylococcus aureus, all panton-valentine leukocidin positive, 7 streptococcus pneumoniae and 1 fusobacterium nucleatum. | 2013 | 23722529 |
| the protective effect of recombinant foma-expressing lactobacillus acidophilus against periodontal infection. | a number of studies have shown that the outer membrane protein foma found in fusobacterium nucleatum demonstrates great potential as an immune target for combating periodontitis. lactobacillus acidophilus is a useful antigen delivery vehicle for mucosal immunisation, and previous studies by our group have shown that l. acidophilus acts as a protective factor in periodontal health. in this study, making use of the immunogenicity of foma and the probiotic properties of l. acidophilus, we construct ... | 2013 | 23644821 |
| a case of adolescent pelvic inflammatory disease caused by a rare bacterium: fusobacterium nucleatum. | pelvic inflammatory disease (pid) is a polymicrobial infection that typically occurs in sexually active females. pid is rare in premenarchal and/or noncoital young women; however, there are case reports of pid occurring in virginal females. we present the first reported case of pid associated with f. nucleatum. in this unusual case of pid in an adolescent, the responsible organism may have originated from the patient's oral cavity. | 2013 | 23619431 |
| microcalorimetric determination of the effects of amoxicillin, metronidazole, and their combination on in vitro biofilm. | the mechanism of action of adjuvant antibiotic therapy in the treatment of peri-implantitis is not well understood. the aim of this study is to investigate antibiotic susceptibility of an in vitro biofilm by isothermal microcalorimetry (imc). | 2014 | 23594193 |
| suppression of t-cell chemokines by porphyromonas gingivalis. | porphyromonas gingivalis is a major pathogen in periodontal disease and is associated with immune dysbiosis. in this study, we found that p. gingivalis did not induce the expression of the t-cell chemokine ip-10 (cxcl10) from neutrophils, peripheral blood mononuclear cells (pbmcs), or gingival epithelial cells. furthermore, p. gingivalis suppressed gamma interferon (ifn-γ)-stimulated release of ip-10, itac (cxcl11), and mig (cxcl9) from epithelial cells and inhibited ip-10 secretion in a mixed i ... | 2013 | 23589576 |
| host susceptibility to periodontitis: mapping murine genomic regions. | host susceptibility to periodontal infection is controlled by genetic factors. as a step toward identifying and cloning these factors, we generated an a/j x balb/cj f2 mouse resource population. a genome-wide search for quantitative trait loci (qtl) associated with periodontitis was performed. we aimed to quantify the phenotypic response of the progenies to periodontitis by microct analysis, to perform a genome-wide search for qtl associated with periodontitis, and, finally, to suggest candidate ... | 2013 | 23539559 |
| regulation of visfatin by microbial and biomechanical signals in pdl cells. | this in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (pdl) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals. | 2014 | 23404558 |
| macrolides inhibit fusobacterium nucleatum-induced muc5ac production in human airway epithelial cells. | fusobacterium nucleatum is one of the most common anaerobic bacteria in periodontitis and is responsible for several extraoral infections, including respiratory tract diseases. in this study, we examined whether f. nucleatum induces mucin secretion in airway epithelial cells. we also examined the effects of macrolides on f. nucleatum-induced mucus production compared with the effects of other antibiotics that exert anti-anaerobic activities. the production of muc5ac, the major core protein of mu ... | 2013 | 23380724 |
| characterization of extracellular polymeric matrix, and treatment of fusobacterium nucleatum and porphyromonas gingivalis biofilms with dnase i and proteinase k. | biofilms are organized communities of microorganisms embedded in a self-produced extracellular polymeric matrix (epm), often with great phylogenetic variety. bacteria in the subgingival biofilm are key factors that cause periodontal diseases; among these are the gram-negative bacteria fusobacterium nucleatum and porphyromonas gingivalis. the objectives of this study were to characterize the major components of the epm and to test the effect of deoxyribonuclease i (dnase i) and proteinase k. | 2013 | 23372876 |
| characteristics of porphyromonas gingivalis lipopolysaccharide in co-culture with fusobacterium nucleatum. | porphyromonas gingivalis is associated with chronic periodontitis and forms multi-species biofilms. they can communicate within species as well as with other species found in the subgingiva, which may induce changes in the growth ratio and virulence of periodontopathogens. the lipopolysaccharide (lps) of p. gingivalis shows different virulence by growth condition. the purpose of this study was to investigate the characteristics of p. gingivalis lps when co-cultured with fusobacterium nucleatum. ... | 2013 | 23347381 |
| clinical and microbiological results following nonsurgical periodontal therapy with or without local administration of piperacillin/tazobactam. | we assessed if adjunct administration of piperacillin/tazobactam added clinical and microbiological treatment benefits. | 2013 | 23334268 |
| exploiting preq(1) riboswitches to regulate ribosomal frameshifting. | knowing the molecular details of the interaction between riboswitch aptamers and their corresponding metabolites is important to understand gene expression. here we report on a novel in vitro assay to study preq(1) riboswitch aptamers upon binding of 7-aminomethyl-7-deazaguanine (preq(1)). the assay is based on the ability of the preq(1) aptamer to fold, upon ligand binding, into a pseudoknotted structure that is capable of stimulating -1 ribosomal frameshifting (-1 fs). aptamers from three diff ... | 2013 | 23327288 |
| dna binding to hydroxyapatite: a potential mechanism for preservation of microbial dna. | molecular methods are increasingly being deployed for analysis of the microbial flora in the root canal. such methods are based on the assumption that recovered dna is associated with the active endodontic infection, yet paleomicrobiology research is based on the recovery of ancient dna from centuries-old tooth and bone samples, which points to considerable longevity of the dna molecule in these tissues. the main component of dentin and bone is the mineral hydroxyapatite. this study assessed dna ... | 2013 | 23321233 |
| bacterial adhesion on the titanium and zirconia abutment surfaces. | microorganisms harboring the oral cavity, mainly those related to periodontal diseases, are the most potential etiologic factor of failure in long-term implant treatment. the material used for abutment components may influence the adhesion and colonization of microbial species. the aim of this in vivo investigation was to evaluate the biofilm formation on machined (mpt) or cast titanium (cpt) and zirconia abutments (zc). | 2014 | 23316996 |
| method to quantify live and dead cells in multi-species oral biofilm by real-time pcr with propidium monoazide. | real-time pcr (qpcr) is a widely used technique in analysing environmental and clinical microbiological samples. however, its main limitation was its inability to discriminate between live and dead cells.recently, propidium monoazide (pma) together with qpcr has been used to overcome this problem, with good results for different bacterial species in different types of samples.our objective was to implement this technique for analysing mortality in multi-species oral biofilms formed in vitro with ... | 2013 | 23289803 |
| presence of oral bacterial species in primary endodontic infections of primary teeth. | knowledge of the microbial composition of deciduous endodontic infections is limited. this study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions. | 2013 | 24683780 |
| isolation and identification of porphyromonas spp. and other putative pathogens from cats with periodontal disease. | the purpose of this study was to evaluate the subgingival microbiota and determine the most prevalent periodontal pathogens implicated in feline periodontal disease and to correlate these findings with the clinical periodontal status. subgingival microbiological samples were taken under sedation from 50 cats with clinical signs of periodontal disease. pooled paper point samples from 4 selected subgingival sites were cultured on blood agar and on dentaid-1 medium. suspected pathogens were identif ... | 2013 | 24660305 |
| fusobacterium nucleatum subsp. fusiforme gharbia and shah 1992 is a later synonym of fusobacterium nucleatum subsp. vincentii dzink et al. 1990. | on the basis of the dna-dna hybridization patterns and phenotypic characteristics, fusobacterium nucleatum was classified into five subspecies. previous studies have suggested that f. nucleatum subsp. vincentii is genetically similar to f. nucleatum subsp. fusiforme. the aim of this study was to investigate the possibility of classifying these two subspecies into a single subspecies by phylogenetic analysis using a single sequence (24,715 bp) concatenated 22 housekeeping genes of eight f. nuclea ... | 2013 | 23263257 |
| virulence mechanism of bacteria in mixed infection: attenuation of cytokine levels and evasion of polymorphonuclear leukocyte phagocytosis. | the objective of the present study is to evaluate the effect of bacterial viability on the virulence of mixed infection. | 2013 | 23259412 |
| purification, crystallization and preliminary x-ray analysis of two hydrogen sulfide-producing enzymes from fusobacterium nucleatum. | hydrogen sulfide produced by oral bacteria is responsible for oral malodour. two homologous hydrogen sulfide-producing enzymes, fn1220 and cdl, from fusobacterium nucleatum (which actively produces hydrogen sulfide) were overproduced, purified and crystallized. x-ray diffraction data were collected from the crystals using a synchrotron-radiation source. the fn1220 crystal belonged to tetragonal space group p4(1)2(1)2 or p4(3)2(1)2 (unit-cell parameters a=b=116.8, c=99.2 å) and the cdl crystal be ... | 2012 | 23192034 |
| prevalence of β-lactamase-producing bacteria in human periodontitis. | beta-lactam antibiotics prescribed in periodontal therapy are vulnerable to degradation by bacterial β-lactamases. this study evaluated the occurrence of β-lactamase-positive subgingival bacteria in chronic periodontitis subjects of usa origin, and assessed their in vitro resistance to metronidazole at a breakpoint concentration of 4 μg/ml. | 2013 | 23173872 |
| high-fat diet induces periodontitis in mice through lipopolysaccharides (lps) receptor signaling: protective action of estrogens. | a fat-enriched diet favors the development of gram negative bacteria in the intestine which is linked to the occurrence of type 2 diabetes (t2d). interestingly, some pathogenic gram negative bacteria are commonly associated with the development of periodontitis which, like t2d, is characterized by a chronic low-grade inflammation. moreover, estrogens have been shown to regulate glucose homeostasis via an lps receptor dependent immune-modulation. in this study, we evaluated whether diet-induced m ... | 2012 | 23133617 |
| diagnosis and treatment of fusobacterium nucleatum discitis and vertebral osteomyelitis: case report and review of the literature. | case report. | 2013 | 23132536 |
| acute chorioamnionitis at term caused by the oral pathogen fusobacterium nucleatum. | fusobacterium nucleatum is an oral pathogen associated with preterm birth. presented is a case of acute chorioamnionitis that progressed to maternal sepsis in a term patient with intact membranes. in addition to its role in periodontal disease and preterm birth, our case demonstrates that intrauterine infection with fusobacterium nucleatum can result in severe disease at term. | 2012 | 23115747 |
| effects of cranberry components on human aggressive periodontitis gingival fibroblasts. | aggressive periodontitis (agp) causes rapid periodontal breakdown involving agp gingival fibroblast production of cytokines [i.e. interleukin (il)-6, a bone metabolism regulator], and matrix metalloproteinase (mmp)-3. lipopolysaccharide upregulates fibroblast il-6 and mmp-3, via transcription factors (i.e. nf-κb). cranberry (vaccinium macrocarpon) inhibits lipopolysaccharide-stimulated macrophage and normal gingival fibroblast activities, but little is known of its effects on agp fibroblasts. ob ... | 2013 | 23106206 |
| draft genome sequence of fusobacterium nucleatum chdc f128, isolated from a periodontitis lesion. | fusobacterium nucleatum is classified into five subspecies. f. nucleatum chdc f128 was isolated from a periodontitis lesion and proposed as a new subspecies based on the comparison of the nucleotide sequences of the rna polymerase beta subunit and zinc protease genes. here, we report the draft genome sequence of the strain. | 2012 | 23105064 |
| inflammatory index and treatment of brain abscess. | this study retrospectively analyzed 12 patients with brain abscesses. half of the patients were diagnosed inaccurately in the initial stage, and 7.2 days were required to achieve the final diagnosis of brain abscess. the patients presented only with a moderately elevated leukocyte count, serum crp levels, or body temperatures during the initial stage. these markers changed, first with an increase in the leukocyte count, followed by the crp and body temperature. the degree of elevation tended to ... | 2012 | 23092104 |
| antibacterial activity of calcium hydroxide and chlorhexidine containing points against fusobacterium nucleatum and parvimonas micra. | up to 90% of the flora of an infected root canal consists of obligate anaerobic bacteria. however, most studies have centred on microaerophiles. this quantitative assay investigated the antibacterial effects against fusobacterium nucleatum and parvimonas micra of gutta-percha (gp) points containing either calcium hydroxide (ca(oh)(2)) or chlorhexidine (chx) compared to those of conventional gp points. | 2012 | 23077425 |
| microscope-based imaging platform for large-scale analysis of oral biofilms. | a microscopic method for noninvasively monitoring oral biofilms at the macroscale was developed to describe the spatial distribution of biofilms of different bacterial composition on bovine enamel surfaces (bes). for this purpose, oral biofilm was grown in situ on bes that were fixed at approximal sites of individual upper jaw acrylic devices worn by a volunteer for 3 or 5 days. eubacteria, streptococcus spp., and fusobacterium nucleatum were stained using specific fluorescence in situ hybridiza ... | 2012 | 23042171 |
| protective potential of non-dialyzable material fraction of cranberry juice on the virulence of p. gingivalis and f. nucleatum mixed infection. | periodontitis is a polymicrobial infectious disease. a novel potential chemical treatment modality may lie in bacterial anti-adhesive materials, such as cranberry juice fractions. the aim of this study is to explore the effect of high molecular weight cranberry constituent (non-dialyzable material [ndm]) on the virulence of a mixed infection with porphyromonas gingivalis and fusobacterium nucleatum in mice. | 2013 | 23030239 |
| morphological, biochemical, physiological and molecular aspects of the response of fusobacterium nucleatum exposed to subinhibitory concentrations of antimicrobials. | subinhibitory concentrations (sics) of antimicrobials may result in alterations in bacterial biology with implications for its potential aggression. this has considerable importance for the resident microbiota. our aim was to analyze the effects of sics of antimicrobials on the morphological, biochemical, physiological and molecular characteristics of the resident anaerobic fusobacterium nucleatum. fourteen strains were obtained from f. nucleatum atcc 25586, selected by culturing on sics of ampi ... | 2012 | 23023263 |
| maternal oral origin of fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16s-23s rrna gene intergenic transcribed spacer region. | fusobacterium nucleatum, a common gram-negative anaerobe prevalent in the oral cavity, possesses the ability to colonize the amniotic cavity and the fetus. however, f. nucleatum may also be part of the vaginal microbiota from where it could reach the amniotic tissues. due to the heterogeneity of f. nucleatum, consisting of five subspecies, analysis at the subspecies/strain level is desirable to determine its precise origin. the aims of this study were: (i) to evaluate the use of the 16s-23s rrna ... | 2013 | 23002071 |
| proteomics of streptococcus gordonii within a model developing oral microbial community. | streptococcus gordonii is one of several species that can initiate the formation of oral biofilms that develop into the complex multispecies microbial communities referred to as dental plaque. it is in the context of dental plaque that periodontal pathogens such as porphyromonas gingivalis cause disease. we have previously reported a whole cell quantitative proteomics investigation of p. gingivalis in a model dental plaque community of s. gordonii, p. gingivalis, and fusobacterium nucleatum. her ... | 2012 | 22989070 |
| isothermal microcalorimetry provides new insights into biofilm variability and dynamics. | the purpose of this study was to investigate a three-species in vitro biofilm with peri-implantitis-related bacteria for its variability and metabolic activity. streptococcus sanguinis, fusobacterium nucleatum, and porphyromonas gingivalis were suspended in simulated body fluid containing 0.2% glucose to form biofilms on polished, protein-coated implant-grade titanium disks over 72 h using a flow chamber system. thereafter, biofilm-coated disks were characterized by scanning electron microscopy ... | 2012 | 22967269 |
| draft genome sequence of fusobacterium nucleatum subsp. fusiforme atcc 51190t. | fusobacterium nucleatum, one of the major causative bacteria of periodontitis, is classified into five subspecies (nucleatum, polymorphum, vincentii, animalis, and fusiforme) on the basis of the several phenotypic characteristics and dna homology. this is the first report of the draft genome sequence of f. nucleatum subsp. fusiforme atcc 51190(t). | 2012 | 22965077 |
| ascorbate and α-tocopherol differentially modulate reactive oxygen species generation by neutrophils in response to fcγr and tlr agonists. | periodontitis, a ubiquitous chronic inflammatory disease, is associated with reduced antioxidant defences and neutrophil hyperactivity in terms of reactive oxygen species (ros) generation. its phenotype is thus characterized by oxidative stress. we have determined the effect of antioxidant micronutrients ascorbate and α-tocopherol on neutrophil ros generation. peripheral neutrophils from periodontally-healthy individuals (n = 20) were challenged with phorbol myristate acetate, igg-opsonised stap ... | 2013 | 22914919 |
| micronutrient modulation of nf-κb in oral keratinocytes exposed to periodontal bacteria. | chronic periodontal diseases are characterised by a dysregulated and exaggerated inflammatory/immune response to plaque bacteria. we have demonstrated previously that oral keratinocytes up-regulate key molecular markers of inflammation, including nf-κb and cytokine signalling, when exposed to the periodontal bacteria porphyromonas gingivalis and fusobacterium nucleatum in vitro. the purpose of the current study was to investigate whether α-lipoic acid was able to abrogate bacterially-induced pro ... | 2013 | 22890546 |
| fusobacterium nucleatum infection mimicking metastatic cancer. | a 48-year-old man presented with fevers, chills, weight loss, multiple liver masses, and several superficial and deep venous thromboses in lower extremities. cancer work up was negative. a liver biopsy grew fusobacterium nucleatum. to our knowledge, f. nucleatum infection presenting with multiple liver masses and trousseau-like syndrome has not been reported earlier. | 2012 | 22875742 |
| comparison of endotoxin levels found in primary and secondary endodontic infections. | this clinical study was conducted to compare the levels of endotoxins (lipopolysaccharides [lpss]) found in primary and secondary endodontic infections with apical periodontitis by correlating lps contents with clinical/radiographic findings. in addition, the presence of target gram-negative anaerobic bacteria was also investigated. | 2012 | 22794210 |
| effectiveness of egg yolk immunoglobulin (igy) against periodontal disease-causing fusobacterium nucleatum. | to evaluate the in vitro and in vivo effectiveness of egg yolk immunoglobulin (igy) against periodontal disease-causing fusobacterium nucleatum. | 2012 | 22789022 |
| peritonsillar abscess: treatment with immediate tonsillectomy - 10 years of experience. | immediate tonsillectomy in patients with peritonsillar abscess is a safe and effective treatment that should be considered as an alternative to conventional incision and drainage. | 2012 | 22708633 |
| effect of s-prg eluate on biofilm formation and enzyme activity of oral bacteria. | recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (s-prg) filler was revealed. we examined the effect of an s-prg eluate on various biologic activities of streptococcus mutans and porphyromonas gingivalis. adherence ability of s. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of p. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. coaggregation of p. gingivalis with ... | 2012 | 22693508 |
| autoinducer 2 of fusobacterium nucleatum as a target molecule to inhibit biofilm formation of periodontopathogens. | periodontitis is initiated by bacteria in subgingival biofilms, which are composed mostly of gram-negative anaerobes. autoinducer 2 (ai-2) is a universal quorum sensing (qs) molecule that mediates intergeneric signalling in multispecies bacterial communities and may induce biofilm formation. as fusobacterium nucleatum is the major coaggregation bridge organism that links early colonising commensals and late pathogenic colonisers in dental biofilms via the accretion of periodontopathogens, we hyp ... | 2013 | 22633049 |
| pulmonary botryomycosis on a lung cavity: a rare pulmonary infection mimicking cancer. | lung botryomycosis is a rare disease. we report what is to our knowledge the first case occurring on a lung cavity. in a 42-year-old man suffering asthenia and cough, a chest radiograph revealed a right upper lobe opacity. computed tomography scan showed a necrotic mass which was also spiculated. repeated research for mycobacterium tuberculosis was negative. the patient underwent a lobectomy. histological and bacteriological examinations made the diagnosis of botryomycosis, because the cavity pr ... | 2012 | 22627958 |
| the foma porin from fusobacterium nucleatum is a toll-like receptor 2 agonist with immune adjuvant activity. | many bacterial components selectively activate immune and nonhematopoietic target cells via toll-like receptor (tlr) signaling; modulation of such host responses defines the immune adjuvant properties of these bacterial products. for example, the outer membrane protein porins from neisseria, salmonella, and shigella are known tlr2 agonists with established systemic and mucosal immune adjuvanticity. early work indicated that the foma porin from fusobacterium nucleatum has immune adjuvant activity ... | 2012 | 22623652 |
| [impact of periodontal disease on arterial pressure in diabetic mice]. | diabetes-driven cardiovascular diseases represent a high challenge for developed countries. periodontal disease is strictly linked to the aforementioned diseases, due to its gram negative-driven inflammation. thus, we investigated the effects of periodontal disease on arterial pressure during the development of diabetes in mice. to this aim, c57bl/6 female mice were colonized with pathogens of periodontal tissue (porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum) for 1m ... | 2012 | 22621847 |
| occurrence and antimicrobial susceptibility of porphyromonas spp. and fusobacterium spp. in dogs with and without periodontitis. | the occurrence of porphyromonas gulae, porphyromonas macacae, fusobacterium nucleatum and fusobacterium canifelinum in subgingival plaque from dogs with and without periodontitis as well as their antimicrobial susceptibility were evaluated. from 50 dogs with periodontitis were identified 38 p. gulae, 8 p. macacae, 26 f. nucleatum and 15 f. canifelinum, and from 50 dogs without periodontitis were identified 15 p. gulae, 12 f. nucleatum and 11 f. canifelinum. all strains were susceptible to most o ... | 2012 | 22609780 |
| the role of coaggregation between porphyromonas gingivalis and fusobacterium nucleatum on the host response to mixed infection. | to evaluate the role of coaggregation between porphyromonas gingivalis and fusobacterium nucleatum on the virulence of the mixed infection in mice. | 2012 | 22607053 |
| septic arthritis and osteomyelitis in a 10-year-old boy, caused by fusobacterium nucleatum, diagnosed with pcr/16s ribosomal bacterial dna amplification. | a 10-year-old boy presented with an atypical non-febrile septic arthritis/osteomyelitis. he was unresponsive to routine antibiotic treatment with flucloxacillin/gentamicin as the pain and fluid collection increased. synovial fluid cultures are negative and gram stain remained negative. only after pcr/16s ribosomal bacterial dna amplification a fusobacterium nucleatum could be detected, and antibiotic therapy switched to clindamycin with rapid response. septic osteomyelitis and arthritis are rela ... | 2012 | 22605875 |
| fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity. | sgp1(t), a strain belonging to a lineage of the phylum synergistetes with no previously cultivated representatives was subjected to a comprehensive range of phenotypic and genotypic tests. for good growth the strain was dependent on co-culture with, or extracts from, selected other oral bacteria. cells of strain sgp1(t) were asaccharolytic and major amounts of acetic acid and moderate amounts of propionic acid were produced as end products of metabolism in peptone-yeast extract-glucose broth sup ... | 2013 | 22493171 |
| butyric acid induces apoptosis in both human monocytes and lymphocytes equivalently. | short-chain fatty acids (scfas) are metabolites from anaerobic periodontopathic bacteria that induce apoptosis in immune cells such as lymphocytes, monocytes and macrophages. however, it remains unclear if scfas from pathogens induce apoptosis in monocytes/macrophages similarly with lymphocytes. this study investigated whether scfas-induced apoptosis is equal among the immunoregulatory cells. cell apoptosis of the employed human cells was evaluated after treatment with culture supernatants from ... | 2012 | 22466881 |
| mycoplasma pneumoniae preceding lemierre's syndrome due to fusobacterium nucleatum complicated by acute epstein-barr virus (ebv) infectious mononucleosis in an immunocompetent host. | we report an unusual case of lemierre's syndrome due to a rare species of fusobacterium, that is, fusobacterium nucleatum preceded by mycoplasma pneumoniae pharyngitis and followed later by epstein-barr virus infectious mononucleosis. | 2013 | 22464641 |
| direct recognition of fusobacterium nucleatum by the nk cell natural cytotoxicity receptor nkp46 aggravates periodontal disease. | periodontitis is a common human chronic inflammatory disease that results in the destruction of the tooth attachment apparatus and tooth loss. although infections with periopathogenic bacteria such as porphyromonas gingivalis (p. gingivalis) and fusobacterium nucleatum (f. nucleatum) are essential for inducing periodontitis, the nature and magnitude of the disease is determined by the host's immune response. here, we investigate the role played by the nk killer receptor nkp46 (ncr1 in mice), in ... | 2012 | 22457623 |
| human neutrophil defensins and their effect on epithelial cells. | the aims of the present study include: 1) to localize human neutrophil defensin-1 (hnp-1) through hnp-3 in gingiva and in neutrophil extract-treated epithelial cell monolayers; 2) to determine the effects of hnp-1 on the epithelial cell viability, attachment, and spreading; and 3) to analyze the effect of hnp-1 on the bacterial adherence to epithelial cells. | 2013 | 22443519 |
| giant frontal mucocele complicated by subdural empyema: treatment of a rare association. | giant frontal mucocele (gfm) is an extremely rare cause of frontal lobe syndrome. subdural empyema (sde) is an uncommon complication of paranasal sinisutis, for which craniotomy and decompressive craniotomy are the most effective surgical procedures. a 54-year-old man was brought unconscious to the emergency room where recurrent generalized seizures occurred. heroine abuse, hcv-related hepatitis, prolonged antibiotic therapy for treatment of purulent rhinorrhea, along with recent personality cha ... | 2012 | 22427297 |
| identification of oral bacterial dna in synovial fluid of patients with arthritis with native and failed prosthetic joints. | we examined the presence of bacterial dna in synovial fluids of native or clinically aseptically failed prosthetic joints from patients having periodontal disease and arthritis to determine whether there is bacterial spread from the oral cavity to the joints. | 2012 | 22426587 |
| effects of oil drops containing lactobacillus salivarius wb21 on periodontal health and oral microbiota producing volatile sulfur compounds. | the objective of this paper is to evaluate the effects of oil drops containing lactobacillus salivarius wb21 on periodontal health and oral microbiota producing volatile sulfur compounds (vscs). for this study, 42 subjects were randomly assigned to receive oil samples containing l. salivarius wb21 or a placebo for two weeks. oral assessment and saliva collection were performed on days 1 and 15. bacterial analysis was performed using the real-time polymerase chain reaction and terminal restrictio ... | 2012 | 22368259 |
| association between oral malodour and periodontal disease-related parameters in the general population. | to determine the association between halitosis detection and periodontal status in systemically healthy non-smokers and to assess whether halitosis was related to quantities of fusobacterium nucleatum and porphyromonas gingivalis on the tongue dorsum. | 2013 | 22339235 |
| detection of fusobacterium nucleatum in chorionic tissues of high-risk pregnant women. | this study was undertaken to investigate the existence of a periodontopathic bacterium, fusobacterium nucleatum, in chorionic tissues of pregnant women, and the effects of f. nucleatum on human chorion-derived cells. | 2012 | 22304677 |
| mucosal invasion by fusobacteria is a common feature of acute appendicitis in germany, russia, and china. | to investigate the geographic occurrence of mucosa-invading fusobacteria in acute appendicitis. | 2015 | 22249094 |
| cytokine expression in response to root canal infection in gnotobiotic mice. | to examine cytokine expression profiles during periapical lesion development in response to synergetic human pathogens in a gnotobiotic mouse model. | 2012 | 22233143 |
| gene-diet interactions on colorectal cancer risk. | there has been increasing interest lately in understanding how natural dietary antioxidants affect chemoprevention, and recently, there has been a merging of information about antioxidants, endogenous and exogenous reactive oxygen and nitrogen species (rons), and inflammation. rons normally serve the cells as second messengers to regulate many of the intracellular signaling cascades that govern multiple cellular activities. however, when the amount of rons exceeds the cell's ability to metaboliz ... | 2012 | 24392268 |
| synergistic growth effect among bacteria recovered from root canal infections. | the objective of this study was to determine the ecological relationships between bacterial species that colonize infected root canals. root canal bacteria recovered from one patient with pulp canal necrosis were evaluated in vitro for synergistic and antagonistic activities determined by mono and co-culture growth kinetics and the production of bacteriocin-like substances using the double layer diffusion method. peptostreptococcus prevotii triggered a significant increase of fusobacterium nucle ... | 2011 | 24031714 |
| canine periodontal disease control using a clindamycin hydrochloride gel. | stabilizing or reducing periodontal pocket depth can have a positive influence on the retention of teeth in dogs. a topical 2% clindamycin hydrochloride gel (chgel) was evaluated for the treatment of periodontal disease in dogs. the chgel formulation provides for the sustained erosion of the matrix, but also flows into the periodontal pocket as a viscous liquid, and then rapidly forms a gel that has mucoadhesive properties and also may function as a physical barrier to the introduction of bacter ... | 2011 | 22416621 |
| vertebral osteomyelitis caused by fusobacterium nucleatum: a case report. | a 58-year-old man with a history of chronic alcohol abuse presented with vertebral osteomyelitis caused by fusobacterium nucleatum. this case demonstrated the foul smelling abscess characteristic of anaerobic infection. deep anaerobic infections are well known to be resistant to closed treatment, and the computerized tomographic scan confirmed the diagnosis of a paravertebral abscess in this case. an increased awareness among clinicians that anaerobic bacilli can cause oteomyelitis, together wit ... | 1979 | 24823152 |
| prevalence of periodontal pathogens among italian patients with chronic periodontitis: a retrospective study on 2992 patients. | the aim of the present study was to evaluate the prevalence of some periodontal pathogens in italian adults with chronic periodontitis. | 2017 | 28757933 |
| do quaternary ammonium monomers induce drug resistance in cariogenic, endodontic and periodontal bacterial species? | antibacterial monomers were developed to combat oral biofilm acids and caries; however, little is known on whether quaternary ammonium monomers (qams) would induce drug resistance in oral bacteria. the objective of this study was to investigate the effects of new antimicrobial monomers dimethylaminohexadecyl methacrylate (dmahdm) and dimethylaminododecyl methacrylate (dmaddm) on the induction of drug resistance in eight species of cariogenic, endodontic and periodontal bacteria for the first tim ... | 2017 | 28755761 |
| herpes simplex 1 and periopathogen role in peri-implantitis. | the objectives of this study were to compare the qualitative and quantitative profiles of herpes simplex virus type i (hsv-1) in implant surfaces between participants with peri-implantitis (pi) and healthy peri-implant tissues and to quantitatively assess the relation between hsv-1 and periopathogens inside the microbiological profile associated with pi. | 2017 | 28512280 |
| porphyromonas gingivalis antibody levels and diagnosis of coronary artery disease in hiv-positive individuals. | periodontal disease has been associated with cardiovascular disease in the general population. it is unknown whether igg antibody levels for periodontal pathogens are associated with the diagnosis of coronary artery disease (cad) in hiv-positive individuals. | 2017 | 28397248 |