| porphyromonas gingivalis initiates a mesenchymal-like transition through zeb1 in gingival epithelial cells. | the oral anaerobe porphyromonas gingivalis is associated with the development of cancers including oral squamous cell carcinoma (oscc). here, we show that infection of gingival epithelial cells with p. gingivalis induces expression and nuclear localization of the zeb1 transcription factor, which controls epithelial-mesenchymal transition. p. gingivalis also caused an increase in zeb1 expression as a dual species community with fusobacterium nucleatum or streptococcus gordonii. increased zeb1 exp ... | 2016 | 26639759 |
| antimicrobial photodynamic therapy (apdt) induction of biofilm matrix architectural and bioadhesive modifications. | dental implants are commonly used today for the treatment of partially and fully edentulous patients. despite the high success rate they are not resistant to complications and failure due to a variety of problems including peri-implantitis or peri-mucositis due to bacterial biofilm formation on the implant surface. the use of non-surgical and surgical treatment procedure to promote healing in cases with peri-implantitis have limited efficacy. here we studied the ability of photodynamic therapy t ... | 2016 | 26617192 |
| hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants. | a versatile strategy to endow dental implants with long-term antibacterial ability without compromising the cytocompatibility is highly desirable to combat implant-related infection. silver nanoparticles (ag nps) have been utilized as a highly effective and broad-spectrum antibacterial agent for surface modification of biomedical devices. however, the high mobility and subsequent hazardous effects of the particles on mammalian cells may limit its practical applications. thus, ag nps were immobil ... | 2015 | 26604743 |
| structural and quantitative analysis of a mature anaerobic biofilm on different implant abutment surfaces. | the longevity of dental implants depends on the absence of inflammation in the periimplant tissue. similar to teeth, pathogenic bacteria can adhere on implant abutment surfaces and cause periimplant disease and consequently implant loss. | 2016 | 26597465 |
| antibacterial efficacy of a cetylpyridinium chloride-based mouthrinse against fusobacterium nucleatum and in vitro plaques. | to assess the antimicrobial effects of a fluoride-free and alcohol-free mouthrinse containing 0.075% cpc (test rinse, tr) compared with an otherwise-identical cpc-free control rinse (cr). | 2015 | 26591620 |
| genotype-specific regulation of oral innate immunity by t2r38 taste receptor. | the bitter taste receptor t2r38 has been shown to regulate mucosal innate immune responses in the upper airway epithelium. furthermore, snps in t2r38 influence the sensitivity to 6-n-propylthiouracil (prop) and are associated with caries risk/protection. however, no study has been reported on the role of t2r38 in the innate immune responses to oral bacteria. we hypothesize that t2r38 regulates oral innate immunity and that this regulation is genotype-specific. primary gingival epithelial cells c ... | 2015 | 26552761 |
| complete genome sequences and analysis of the fusobacterium nucleatum subspecies animalis 7-1 bacteriophage ɸfunu1 and ɸfunu2. | fusobacterium nucleatum is a strictly anaerobic, gram negative bacterial species that has been associated with dental infections, pre-term labor, appendicitis, inflammatory bowel disease, and, more recently, colorectal cancer. the species is unusual in its phenotypic and genotypic heterogeneity, with some strains demonstrating a more virulent phenotype than others; however, as yet the genetic basis for these differences is not understood. bacteriophage are known to contribute to the virulence ph ... | 2016 | 26545740 |
| subgingival plaque in periodontal health antagonizes at toll-like receptor 4 and inhibits e-selectin expression on endothelial cells. | the ability of the subgingival microbial community to induce an inappropriate inflammatory response ultimately results in the destruction of bone and gingival tissue. in this study, subgingival plaque samples from both healthy and diseased sites in the same individual were obtained from adults with chronic periodontitis and screened for their ability to either activate toll-like receptor 2 (tlr2) or tlr4 and to antagonize tlr4-specific activation by agonist, fusobacterium nucleatum lps. subgingi ... | 2015 | 26483407 |
| antimicrobial effectiveness of cetylpyridinium chloride and zinc chloride-containing mouthrinses on bacteria of halitosis and peri-implant disease. | to clarify the antimicrobial efficacy of zinc chloride (zncl2) and cetylpyridinium chloride (cpc) by testing their impact on the growth of seven bacterial strains known to be involved in the pathophysiology of both peri-implant disease and halitosis-staphylococcus aureus, streptococcus mutans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, treponema denticola, and tannerella forsythia. | 2015 | 26478974 |
| microbiological effects and recolonization patterns after adjunctive subgingival debridement with er:yag laser. | the objective of this study was to assess the microbiological effects and recolonization patterns after non-surgical periodontal treatment protocol based on the adjunctive use of erbium-doped yttrium aluminium garnet (er:yag) laser. | 2016 | 26419675 |
| slowly progressive lemierre's syndrome with orbital pain and exophthalmos. | lemierre's syndrome is an oropharyngeal bacterial infection characterized by rapidly progressive septic thrombophlebitis of the internal jugular vein. a lack of appropriate antibiotic therapy can be life-threatening. we describe the case of a 39-year-old man with lemierre's syndrome who presented with long-lasting orbital pain and acute exophthalmos 6 weeks after initial infection. this report is to help clinicians consider the diagnosis of lemierre's syndrome when encountering a patient with lo ... | 2016 | 26419364 |
| long-term evaluation of oral gavage with periodontopathogens or ligature induction of experimental periodontal disease in mice. | to evaluate in long-term periods the destruction of periodontal tissues and bacterial colonization induced by oral gavage with periodontopathogens or ligature experimental periodontal disease models. | 2016 | 26411857 |
| metagenomic analysis of faecal microbiome as a tool towards targeted non-invasive biomarkers for colorectal cancer. | to evaluate the potential for diagnosing colorectal cancer (crc) from faecal metagenomes. | 2017 | 26408641 |
| fusobacterium nucleatum osteomyelitis in 3 previously healthy children: a case series and review of the literature. | fusobacterium nucleatum is a rare cause of monomicrobial osteomyelitis in children. we describe the cases of 3 children with f. nucleatum osteomyelitis of the distal femoral epiphysis with concurrent septic arthritis and review 6 other cases reported in the literature. our report emphasizes the importance of maintaining a high index of suspicion for anaerobic osteomyelitis, given its atypical presentation and the unique growth requirements of anaerobic bacteria. | 2015 | 26407282 |
| "messieurs, c'est les microbes qui auront le dernier mot": gentlemen, it is the microbes who have the last word (louis pasteur)-fusobacterium nucleatum protect tumors from killing by immune cells. | fusobacterium nucleatum is present in colon cancers where it was shown to generate a proinflammatory microenvironment that supports colorectal neoplasia progression. remarkably, alongside with proinflammatory stimulation, fusobacteria also inhibit cytotoxicity of immune cells. thus, it appears as if tumors exploit fusobacteria to generate a favorable proinflammatory and anti-cytotoxic microenvironment. | 2015 | 26405611 |
| berberine may rescue fusobacterium nucleatum-induced colorectal tumorigenesis by modulating the tumor microenvironment. | accumulating evidence links colorectal cancer (crc) with the intestinal microbiota. however, the disturbance of intestinal microbiota and the role of fusobacterium nucleatum during the colorectal adenoma-carcinoma sequence have not yet been evaluated. | 2015 | 26397137 |
| endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation. | this study aims to assess the potential of a mixture of three antibiotics (trevitamix, tvm) as an intracanal dressing to disinfect the outer root surface by applying a new in vitro model. | 2016 | 26323501 |
| sialic acid transporter nant participates in tannerella forsythia biofilm formation and survival on epithelial cells. | tannerella forsythia is a periodontal pathogen implicated in periodontitis. this gram-negative pathogen depends on exogenous peptidoglycan amino sugar n-acetylmuramic acid (nam) for growth. in the biofilm state the bacterium can utilize sialic acid (neu5ac) instead of nam to sustain its growth. thus, the sialic acid utilization system of the bacterium plays a critical role in the growth and survival of the organism in the absence of nam. we sought the function of a t. forsythia gene annotated as ... | 2016 | 26318875 |
| fusobacterium nucleatum in colorectal carcinoma tissue and patient prognosis. | accumulating evidence links the intestinal microbiota and colorectal carcinogenesis. fusobacterium nucleatum may promote colorectal tumour growth and inhibit t cell-mediated immune responses against colorectal tumours. thus, we hypothesised that the amount of f. nucleatum in colorectal carcinoma might be associated with worse clinical outcome. | 2016 | 26311717 |
| in vitro activity of taurolidine gel on bacteria associated with periodontitis. | the purpose of this in vitro study was to determine the antimicrobial activity of two different taurolidine gel formulations in comparison with minocycline microspheres. | 2016 | 26254142 |
| lethal effect of blue light-activated hydrogen peroxide, curcumin and erythrosine as potential oral photosensitizers on the viability of porphyromonas gingivalis and fusobacterium nucleatum. | recently, photodynamic therapy (pdt) has been introduced as a new modality in oral bacterial decontamination. current research aims to evaluate the effect of photodynamic killing of visible blue light in the presence of hydrogen peroxide, curcumin and erythrosine as potential oral photosensitizers on porphyromonas gingivalis associated with periodontal bone loss and fusobacterium nucleatum associated with soft tissue inflammation. | 2015 | 26246690 |
| inactivating effects of the lactoperoxidase system on bacterial lyases involved in oral malodour production. | the main components of oral malodour have been identified as volatile sulfur compounds (vscs), including hydrogen sulfide (h(2)s) and methyl mercaptan (ch(3)sh). the lactoperoxidase (lpo) system (consisting of lpo, glucose oxidase, glucose and thiocyanate) was previously shown to exhibit antimicrobial activities against some oral bacteria in vitro and suppressive effects on vscs in mouth air in a clinical trial. here, we examined the in vitro effects of the lpo system on the activities of the ba ... | 2015 | 26242770 |
| efficacy of a novel antimicrobial peptide against periodontal pathogens in both planktonic and polymicrobial biofilm states. | streptococcus gordonii, fusobacterium nucleatum and porphyromonas gingivalis represent the early, middle and late colonizers of the bacterial accretion in dental plaque biofilms. these sessile communities constitute a protected mode of growth that promotes survival in a hostile environment. this study describes a novel and unrecognized role for a synthetic cationic antimicrobial peptide, nal-p-113, which inhibits and kills periodontal bacteria in planktonic state, inhibits the formation of biofi ... | 2015 | 26210284 |
| wild blueberry (vaccinium angustifolium ait.) polyphenols target fusobacterium nucleatum and the host inflammatory response: potential innovative molecules for treating periodontal diseases. | blueberries contain significant amounts of flavonoids to which a number of beneficial health effects in humans have been associated. the present study investigated the effect of a polyphenol-rich lowbush blueberry (vaccinium angustifolium ait.) extract on the two main etiologic components of periodontitis, a multifactorial disorder affecting the supporting structures of the teeth. phenolic acids, flavonoids (flavonols, anthocyanins, flavan-3-ols), and procyanidins made up 16.6, 12.9, and 2.7% of ... | 2015 | 26207764 |
| fusobacterium nucleatum and t cells in colorectal carcinoma. | evidence indicates a complex link between gut microbiome, immunity, and intestinal tumorigenesis. to target the microbiota and immunity for colorectal cancer prevention and therapy, a better understanding of the relationship between microorganisms and immune cells in the tumor microenvironment is needed. experimental evidence suggests that fusobacterium nucleatum may promote colonic neoplasia development by downregulating antitumor t cell-mediated adaptive immunity. | 2015 | 26181352 |
| periodontal pathogens porphyromonas gingivalis and fusobacterium nucleatum promote tumor progression in an oral-specific chemical carcinogenesis model. | oral squamous cell carcinoma (oscc) is a lethal disease whose incidence is increasing. epidemiologic studies demonstrate an association between periodontitis and oral cancer, and periodontal pathogens are implicated in the pathogenesis of numerous disorders, including rheumatoid arthritis, cardiovascular diseases, diabetes and gastrointestinal malignancies. nevertheless, a causal role for periodontal pathogens in oscc has not been shown, partly due to the lack of an appropriate animal model. her ... | 2015 | 26158901 |
| influence of the prosthetic index into morse taper implants on bacterial microleakage. | to evaluate the influence of morse taper implant index on microleakage. | 2015 | 26068320 |
| mechanistic analysis of plga/hpmc-based in-situ forming implants for periodontitis treatment. | in-situ forming implant formulations based on poly(lactic-co-glycolic acid) (plga), acetyltributyl citrate (atbc), minocycline hcl, n-methyl pyrrolidone (nmp) and optionally hydroxypropyl methylcellulose (hpmc) were prepared and thoroughly characterized in vitro. this includes electron paramagnetic resonance (epr), nuclear magnetic resonance ((1)h nmr), mass change and drug release measurements under different conditions, optical microscopy, size exclusion chromatography (sec) as well as antibac ... | 2015 | 26047797 |
| [a case of corticosteroid-responsive lemierre syndrome with clivus osteomyelitis and a mass in the cavernous sinus-suprasellar region]. | lemierre syndrome is a clinical syndrome that presents with internal jugular thrombophlebitis, septicemia and systemic abscess formations. in general, the condition is preceded by oropharyngeal infections. we report a case of a 73-year-old man with lemierre syndrome, clivus osteomyelitis and a steroid-responsive mass in the cavernous sinus-suprasellar region. he complained of fever, occipital pain, diplopia and right ptosis. administration of oral steroids ameliorated the ophthalmic symptoms for ... | 2015 | 26028195 |
| [spondylodiscitis due to fusobacterium nucleatum: new diagnostic method]. | fusobacterium spp. are gram negative anaerobe bacteria. vertebral osteomyelitis caused by these bacteria is very unusual; in fact, we could only find 11 cases in the literature. we report the case of a male, 46 year-old patient who had had lumbar pain for several weeks that irradiated to the right leg, and did not respond to nsaid treatment. the work-up included mri, biopsy with draining of the collection and a universal pcr followed by 16s rdna sequencing. the latter was used to make the microb ... | 2015 | 26021107 |
| a clinical study evaluating changes in the microbial flora around dental implants during various stages of implant restoration. | this study evaluated the microbial colonization of dental implants in healthy patients before placing the implants and during the various stages of implant treatment. | 2015 | 25996787 |
| real-time monitoring of the metabolic activity of periodontopathic bacteria. | bacterial metabolic activity is associated with the onset and progression mechanisms of oral biofilm-mediated disease; however, at present no method to monitor bacterial metabolism exists, especially for periodontopathic bacteria. therefore, we aimed to establish a novel method for monitoring the metabolic activity of periodontopathic bacteria, porphyromonas gingivalis (pg), prevotella intermedia (pi) and fusobacterium nucleatum (fn), as well as streptococcus mutans (sm) for comparison. the meth ... | 2015 | 25986950 |
| liquid based microbiological transport systems: conformity assessment of two commercial devices. | we compared two types of liquid-based microbiology devices for microorganism viability according to standardized quantitative elution method clsi m40-a2. the eswab® met clsi acceptance criteria of viability maintenance for all microorganisms tested. the σ-transwab® failed to meet clsi acceptance criteria for peptostreptococcus anaerobius, prevotella melaninogenica, fusobacterium nucleatum and haemophilus influenzae. | 2015 | 25957512 |
| internal jugular vein septic thrombophlebitis (lemierre syndrome) as a complication of pharyngitis. | sore throat is a common presenting complaint in the outpatient setting. most cases are nonbacterial in origin, but those that are bacterial are usually the result of group a β-hemolytic streptococcus. guidelines exist to help physicians decide whether to treat with an antibiotic. lemierre syndrome is a dangerous potential sequela of pharyngitis that results in septic thrombophlebitis of the internal jugular (ij) vein. a high index of suspicion is needed to consider this diagnosis in the workup o ... | 2015 | 25957375 |
| pyrosequencing analysis of subgingival microbiota in distinct periodontal conditions. | subgingival microorganisms are potentially associated with periodontal diseases. however, changes in the subgingival microbiota during the progress of periodontal diseases are poorly understood. in this study, we analyzed bacterial communities in the subgingival paper point samples from 32 korean individuals with no sign of disease, gingivitis, or periodontitis using 454 flx titanium pyrosequencing. a total of 256,113 reads representing 26 phyla, 433 genera, and 1,016 species were detected. bact ... | 2015 | 25904141 |
| pyogenic liver abscess caused by fusobacterium in a 21-year-old immunocompetent male. | a 21-year-old male with no significant past medical history, presented with right upper quadrant (ruq) abdominal pain along with fevers and chills. lab work revealed leukocytosis, anemia, and slightly elevated alkaline phosphatase. viral serology for hepatitis b virus, hepatitis c virus, and human immunodeficiency virus were negative and he was immunocompetent. computed tomography imaging revealed hepatic abscesses, the largest measuring 9.5 cm. empiric antibiotics were started and percutaneous ... | 2015 | 25834342 |
| short-chain fatty acids in infected root canals of teeth with apical periodontitis before and after treatment. | short-chain fatty acids (scfas) are bacterial metabolic end products that may function as virulence factors. this study evaluated the occurrence of scfas in infected root canals before and after treatment. | 2015 | 25799538 |
| the effect of blue light on periodontal biofilm growth in vitro. | we have previously shown that blue light eliminates the black-pigmented oral bacteria porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens, and prevotella melaninogenica. in the present study, the in vitro photosensitivity of the above black-pigmented microorganisms and four fusobacteria species (fusobacterium nucleatum ss. nucleatum, f. nucleatum ss. vincentii, f. nucleatum ss. polymorphum, fusobacterium periodonticum) was investigated in pure cultures and human dental plaque ... | 2015 | 25759232 |
| subacute osteomyelitis of the femur due to fusobacterium nucleatum in a 7-year-old boy. | subacute hematogenous osteomyelitis is an insidious infection, which commonly has a delayed diagnosis. we describe the case of a 7-year-old boy with subacute osteomyelitis, which was initially considered to be a bone tumor. infection should be considered in all cases of bone pain, especially in children, even in the absence of typical systemic features of inflammation. | 2015 | 25742081 |
| dna methylation differentially regulates cytokine secretion in gingival epithelia in response to bacterial challenges. | epigenetic modifications are changes in gene expression without altering dna sequence. we previously reported that bacteria-specific innate immune responses are regulated by epigenetic modifications. our hypothesis is that dna methylation affects gingival cytokine secretion in response to bacterial stimulation. gingival epithelial cells (gecs) were treated with dnmt-1 inhibitors prior to porphyromonas gingivalis (pg) or fusobacterium nucleatum (fn) exposure. protein secretion was assessed using ... | 2015 | 25722484 |
| oral bacteria adherence to suture threads: an in vitro study. | this study investigated the adherence of oral microorganisms to different types of suture threads. | 2015 | 25711725 |
| high in vitro antibacterial activity of pac-525 against porphyromonas gingivalis biofilms cultured on titanium. | in order to investigate the potential of short antimicrobial peptides (amps) as alternative antibacterial agents during the treatment of peri-implantitis, the cytotoxic activity of three short amps, that is, pac-525, ksl-w, and ksl, was determined using the mtt assay. the antimicrobial activity of these amps, ranging in concentration from 0.0039 mg/ml to 0.5 mg/ml, against the predominant planktonic pathogens, including streptococcus sanguis, fusobacterium nucleatum, and porphyromonas gingivalis ... | 2015 | 25710035 |
| killing dental pathogens using antibacterial graphene oxide. | dental caries and periodontal diseases have a close relationship with microbes such as streptococcus mutans, porphyromonas gingivalis and fusobacterium nucleatum. graphene oxide (go), as the derivative of graphene, plays an important role in many areas including biology and medicine. in particular, it has been known as a promising antimicrobial nanomaterial. in this study, we focused on the antimicrobial property of go against dental pathogens. with the utilization of 3-(4,5-dimethylthiazol-2-yl ... | 2015 | 25705785 |
| bactericidal effect of visible light in the presence of erythrosine on porphyromonas gingivalis and fusobacterium nucleatum compared with diode laser, an in vitro study. | recently, photodynamic therapy (pdt) has been introduced as a new modality in oral bacterial decontamination. besides, the ability of laser irradiation in the presence of photosensitizing agent to lethal effect on oral bacteria is well documented. current research aims to evaluate the effect of photodynamic killing of visible blue light in the presence of plaque disclosing agent erythrosine as photosensitizer on porphyromonas gingivalis associated with periodontal bone loss and fusobacterium nuc ... | 2014 | 25705082 |
| association of fusobacterium nucleatum with clinical and molecular features in colorectal serrated pathway. | human gut microbiota is being increasingly recognized as a player in colorectal cancers (crcs). evidence suggests that fusobacterium nucleatum (f. nucleatum) may contribute to disease progression and is associated with cpg island methylator phenotype (cimp) and microsatellite instability (msi) in crcs; however, to date, there are no reports about the relationship between f. nucleatum and molecular features in the early stage of colorectal tumorigenesis. therefore, we investigated the presence of ... | 2015 | 25703934 |
| an oral commensal associates with disease: chicken, egg, or red herring? | fusobacterium nucleatum might be the cause or consequence of disease in many tissues in and outside the mouth. in this issue of immunity, gur et al. (2015) suggest a new mechanism by which this oral commensal might help cancer cells escape host immunity. | 2015 | 25692696 |
| binding of the fap2 protein of fusobacterium nucleatum to human inhibitory receptor tigit protects tumors from immune cell attack. | bacteria, such as fusobacterium nucleatum, are present in the tumor microenvironment. however, the immunological consequences of intra-tumoral bacteria remain unclear. here, we have shown that natural killer (nk) cell killing of various tumors is inhibited in the presence of various f. nucleatum strains. our data support that this f. nucleatum-mediated inhibition is mediated by human, but not by mouse tigit, an inhibitory receptor present on all human nk cells and on various t cells. using a lib ... | 2015 | 25680274 |
| evaluation of holy basil mouthwash as an adjunctive plaque control agent in a four day plaque regrowth model. | various antibacterial and antiplaque agents are used in chemical plaque control but none are without their shortcomings. chlorhexidine considered a gold standard, also has an array of side effects. to overcome these, numerous herbal extracts have been tried and tested and one among them is holy basil. the present study evaluated the antibacterial efficacy of holy basil in vitro against some periodontopathogens and its antiplaque effect in vivo. | 2014 | 25674314 |
| [cerebellar abscess due to infection with the anaerobic bacteria fusobacterium nucleatum: a case report]. | we report a rare case of cerebellar abscess produced by anaerobic bacteria. a 76-year-old man was admitted to our hospital with a history of fever, vomiting, and dizziness lasting 14 days. computed tomography(ct)scan and magnetic resonance images showed the presence of a multiloculated cerebellar abscess with a right subdural abscess. the patient underwent aspiration of the abscess through a suboccipital craniotomy. fusobacterium nucleatum, which is an anaerobic bacteria naturally present in the ... | 2015 | 25672555 |
| changes in microflora in dental plaque from cancer patients undergoing chemotherapy and the relationship of these changes with mucositis: a pilot study. | to assess changes in oral microflora in dental plaque from cancer patients within 7 days of the first course of chemotherapy, and the relationship of the changes with mucositis. | 2015 | 25662538 |
| novel therapeutic approach for the treatment of periodontitis by curcumin. | the aim of the present study was to evaluate the clinical and microbiological efficacy of locally delivered 1% curcumin gel as an adjunct to scaling and root planing in the treatment of chronic periodontitis. | 2014 | 25654035 |
| fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | we explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) for identification of fusobacterium nucleatum subspecies. maldi-tof ms spectra of five f. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. maldi-tof ms with the modified saramis database further correctly identified 28 of ... | 2015 | 25653408 |
| 16s rrna based microarray analysis of ten periodontal bacteria in patients with different forms of periodontitis. | dna microarray analysis is a computer based technology, that a reverse capture, which targets 10 periodontal bacteria (parocheck) is available for rapid semi-quantitative determination. the aim of this three-year retrospective study was to display the microarray analysis results for the subgingival biofilm samples taken from patient cases diagnosed with different forms of periodontitis. a total of 84 patients with generalized aggressive periodontitis (gap,n:29), generalized chronic periodontitis ... | 2015 | 25638399 |
| [research progress in fada from fusobacterium nucleatum]. | | 2014 | 25619301 |
| fusobacterium nucleatum endocarditis presenting as liver and brain abscesses in an immunocompetent patient. | | 2015 | 25588024 |
| microbial diversity of the supra- and subgingival biofilm of healthy individuals after brushing with chlorhexidine- or silver-coated toothbrush bristles. | nanoparticulate silver has recently been reported as an effective antimicrobial agent. the aim of this clinical study was to investigate the potential changes on the oral microbiota of healthy individuals after controlled brushing with chlorhexidine- or silver-coated toothbrush bristles. twenty-four healthy participants were enrolled in this investigation and randomly submitted to 3 interventions. all the participants received, in a crossover format, the following toothbrushing interventions: (i ... | 2015 | 25581046 |
| fusobacterium nucleatum: a commensal-turned pathogen. | fusobacterium nucleatum is an anaerobic oral commensal and a periodontal pathogen associated with a wide spectrum of human diseases. this article reviews its implication in adverse pregnancy outcomes (chorioamnionitis, preterm birth, stillbirth, neonatal sepsis, preeclampsia), gi disorders (colorectal cancer, inflammatory bowel disease, appendicitis), cardiovascular disease, rheumatoid arthritis, respiratory tract infections, lemierre's syndrome and alzheimer's disease. the virulence mechanisms ... | 2015 | 25576662 |
| one-step purification and porin transport activity of the major outer membrane proteins p2 from haemophilus influenzae, foma from fusobacterium nucleatum and porb from neisseria meningitidis. | bacterial porins are major outer membrane proteins that function as essential solute transporters between the bacteria and the extracellular environment. structural features of porins are also recognized by eukaryotic cell receptors involved in innate and adaptive immunity. to better investigate the function of porins, proper refolding is necessary following purification from inclusion bodies [1, 2]. using a single-step size exclusion chromatographic method, we have purified three major porins f ... | 2015 | 25575589 |
| fusobacterium nucleatum: an emerging bug in colorectal tumorigenesis. | the human intestinal microbiota is a plethora of diverse microbial species, wherein certain bacteria considered as driver bacteria with procarcinogenic features contribute directly toward colonic epithelium cell damage to initiate colorectal carcinogenesis. however, some bacteria, in particular fusobacterium nucleatum, which is otherwise a normal resident of the oral microflora and a relatively poor colonizer of the healthy gut, have also been considered to play a role in the development of colo ... | 2015 | 25569450 |
| intracanal antibiotic medication for sustained root surface disinfection-a laboratory evaluation. | to measure the release of an antibiotic mixture of ciprofloxacin, cerfuroxim and metronidazole (trevitamix, tvm) through human dentine and to assess the growth inhibition of fusobacterium nucleatum. | 2015 | 26966464 |
| myopericarditis associated with fusobacterium nucleatum-caused liver abscess. | a wide clinical spectrum of bacteremic disease caused by fusobacterium has been presented in this journal. we wish to extend this spectrum by presenting a case of myopericarditis resulting from a liver abscess caused by f. nucleatum. while f. nucleatum plays an important role in periodontal disease, and has been isolated from skin ulcers, liver abscesses, urinary tract infections, and endocarditis, a single case of f. nucleatum-induced pericarditis is documented in the literature. | 2015 | 25530149 |
| oral gram-negative anaerobic bacilli as a reservoir of β-lactam resistance genes facilitating infections with multiresistant bacteria. | many β-lactamases have been described in various gram-negative bacilli (capnocytophaga, prevotella, fusobacterium, etc.) of the oral cavity, belonging to class a of the ambler classification (cepa, cbla, cfxa, csp-1 and tem), class b (cfia) or class d in fusobacterium nucleatum (fus-1). the minimum inhibitory concentrations of β-lactams are variable and this variation is often related to the presence of plasmids or other mobile genetic elements (mges) that modulate the expression of resistance g ... | 2015 | 25465519 |
| interactions of anaerobic bacteria with dental stem cells: an in vitro study. | in patients with periodontitis, it is highly likely that local (progenitor) cells encounter pathogenic bacteria. the purpose of this in vitro study was to elucidate how human dental follicle stem cells (hdfsc) react towards a direct challenge with anaerobic periodontal pathogens under their natural oxygen-free atmosphere. hdfsc were compared to human bone marrow mesenchymal stem cells (hbmsc) and differentiated primary human gingival fibroblasts (hgif), as well as permanent gingival carcinoma ce ... | 2014 | 25369260 |
| identification and characterization of fusolisin, the fusobacterium nucleatum autotransporter serine protease. | fusobacterium nucleatum is an oral anaerobe associated with periodontal disease, adverse pregnancy outcomes and colorectal carcinoma. a serine endopeptidase of 61-65 kda capable of damaging host tissue and of inactivating immune effectors was detected previously in f. nucleatum. here we describe the identification of this serine protease, named fusolisin, in three oral f. nucleatum sub-species. gel zymogram revealed fusobacterial proteolytic activity with molecular masses ranging from 55-101 kda ... | 2014 | 25357190 |
| [effects of cetylpyridinium chloride buccal tablets on halitosis induced by oral conditions]. | to investigate the effect of cetylpyridinium chloride buccal tablets on halitosis induced by oral conditions. | 2014 | 25263382 |
| global analysis of saliva as a source of bacterial genes for insights into human population structure and migration studies. | the genetic diversity of the human microbiome holds great potential for shedding light on the history of our ancestors. helicobacter pylori is the most prominent example as its analysis allowed a fine-scale resolution of past migration patterns including some that could not be distinguished using human genetic markers. however studies of h. pylori require stomach biopsies, which severely limits the number of samples that can be analysed. by focussing on the house-keeping gene gdh (coding for the ... | 2014 | 25183372 |
| proteomics of fusobacterium nucleatum within a model developing oral microbial community. | fusobacterium nucleatum is a common oral organism that can provide adhesive and metabolic support to developing periodontal bacterial communities. it is within the context of these communities that disease occurs. we have previously reported whole cell proteomics analyses of porphyromonas gingivalis and streptococcus gordonii in early-stage communities with each other and with f. nucleatum, modeled using 18 h pellets. here, we report the adaptation of f. nucleatum to the same experimental condit ... | 2014 | 25155235 |
| increased proliferation and decreased membrane permeability as defense mechanisms of fusobacterium nucleatum against human neutrophilic peptide-1. | human neutrophilic peptides (hnps) constitute a class of host defense molecules, which contribute to the non-oxidative killing of bacteria and other microorganisms. since the adaptability is crucial to bacterial survival in changing environments, it is of interest to know how fusobacterium nucleatum, the major bridge organism connecting early and late colonizers in dental biofilms, defends itself against hnps. this study aimed to examine the planktonic growth, membrane permeability, and biofilm ... | 2014 | 25132418 |
| fusobacterium detected in colonic biopsy and clinicopathological features of ulcerative colitis in japan. | fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. here, we performed prevalence screening of fusobacterium in ulcerative colitis (uc) in japanese patients. | 2015 | 25102986 |
| reducing the bioactivity of tannerella forsythia lipopolysaccharide by porphyromonas gingivalis. | tannerella forsythia is considered a pathogen of periodontitis and forms a biofilm with multi-species bacteria in oral cavity. lipopolysaccharide is a powerful immunostimulator and induces inflammation and shock. the purpose of this study was to investigate the characteristics of t. forsythia lps in its co-cultivation with fusobacterium nucleatum or porphyromonas gingivalis. t. forsythia was co-cultured in the presence and absence of f. nucleatum and p. gingivalis and then t. forsythia lps was e ... | 2014 | 25098564 |
| active invasion of porphyromonas gingivalis and infection-induced complement activation in apoe-/- mice brains. | periodontal disease is a polymicrobial inflammatory disease that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute to the development of alzheimer's disease. apoe-/- mice were orally infected (n = 12) with porphyromonas gingivalis, treponema denticola, tannerella forsythia, and fusobacterium nucleatum as mono- and polymicrobial infections. apoe-/- mice were sacrificed following 12 and 24 weeks of chronic infection. bacterial gen ... | 2015 | 25061055 |
| pathogenic bacterial species associated with endodontic infection evade innate immune control by disabling neutrophils. | endodontic infections, in which oral bacteria access the tooth pulp chamber, are common and do not resolve once established. to investigate the effects of these infections on the innate immune response, we established a mouse subcutaneous chamber model, where a mixture of four oral pathogens commonly associated with these infections (endodontic pathogens [ep]), i.e., fusobacterium nucleatum, streptococcus intermedius, parvimonas micra, and prevotella intermedia, was inoculated into subcutaneousl ... | 2014 | 25024367 |
| bacterial periplasmic sialic acid-binding proteins exhibit a conserved binding site. | sialic acids are a family of related nine-carbon sugar acids that play important roles in both eukaryotes and prokaryotes. these sialic acids are incorporated/decorated onto lipooligosaccharides as terminal sugars in multiple bacteria to evade the host immune system. many pathogenic bacteria scavenge sialic acids from their host and use them for molecular mimicry. the first step of this process is the transport of sialic acid to the cytoplasm, which often takes place using a tripartite atp-indep ... | 2014 | 25004958 |
| biomechanical loading modulates proinflammatory and bone resorptive mediators in bacterial-stimulated pdl cells. | the present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (pdl) cells in the presence of bacterial challenge. cells were seeded on bioflex culture plates and exposed to fusobacterium nucleatum atcc 25586 and/or cyclic tensile strain (cts) of low (ctsl) and high (ctsh) magnitudes for 1 and 3 days. synthesis of cyclooxygenase-2 (cox2) and prostaglandin e2 (pge2) was evaluated by elisa. gene ... | 2014 | 24976684 |
| identification of occult fusobacterium nucleatum central nervous system infection by use of pcr-electrospray ionization mass spectrometry. | anaerobic bacteria are often difficult to detect, especially after the initiation of antibiotics. we describe the application of pcr-electrospray ionization mass spectrometry (pcr/esi-ms) using a sample of cerebrospinal fluid to identify an anaerobic gram-negative bacillus, fusobacterium nucleatum, in a patient with "culture-negative" meningitis and cerebral abscesses. | 2014 | 24966358 |
| gastrointestinal variant of lemierre syndrome: fusobacterium nucleatum bacteremia-associated hepatic vein thrombosis: a case report and literature review. | fusobacterium nucleatum is a gram-negative bacillius commonly found in oropharynx and is traditionally associated with lemierre syndrome, which is characterized by history of recent oropharyngeal infection, internal jugular vein thrombosis, and isolation of anaerobic pathogens, mainly fuosobacterium necrophorum. however, recent evidence indicated that f. nucleatum is also a normal resident of human gut. less than a dozen of case reports had linked f. nucleatum to gastrointestinal variant of lemi ... | 2017 | 24942004 |
| in vitro efficacy of cefovecin against anaerobic bacteria isolated from subgingival plaque of dogs and cats with periodontal disease. | periodontal disease is a common disease of dogs and cats often requiring antimicrobial treatment as an adjunct to mechanical debridement. however, correct compliance with oral antimicrobial therapy in companion animals is often difficult. cefovecin is a recently introduced veterinary cephalosporin that has demonstrated prolonged concentrations in extracellular fluid, allowing for dosing intervals of up to 14 days. subgingival samples were collected from the oral cavity of 29 dogs and eight cats ... | 2014 | 24930431 |
| effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria. | little is known about the effects of stress hormones on the etiologic agents of halitosis. thus, the aim of this study was to evaluate in vitro the effects of adrenaline (adr), noradrenaline (na) and cortisol (cort) on bacteria that produce volatile sulfur compounds (vsc), the major gases responsible for bad breath. cultures of fusobacterium nucleatum (fn), porphyromonas endodontalis (pe), prevotella intermedia (pi) and porphyromonas gingivalis (pg) were exposed to 50 µm adr, na and cort or equi ... | 2014 | 24918364 |
| rapid brain death caused by a cerebellar abscess with fusobacterium nucleatum in a young man with drug abuse: a case report. | fusobacterium nucleatum is a strict anaerobic microorganism that causes disease entities such as periodontal and soft tissue abscesses, pulmonary and intraabdominal infections and very rarely intracerebral infections. | 2014 | 24915846 |
| ubiquitous sialometabolism present among oral fusobacteria. | fusobacterium nucleatum is a ubiquitous member of the human oral flora and is associated with the development of periodontitis and a variety of other types of polymicrobial infections of the mucosa. in the oral cavity, this species is one of the few that is prevalent in both healthy and diseased subgingival plaque. using microarray analysis, we examined the transcriptional response of f. nucleatum subspecies nucleatum to whole blood in order to identify some of the genetic responses that might o ... | 2014 | 24896093 |
| the antibiofilm activity of lingonberry flavonoids against oral pathogens is a case connected to residual complexity. | the antimicrobial activity of lingonberry (vaccinium vitis-idaea l.) was evaluated against two oral pathogens, streptococcus mutans and fusobacterium nucleatum. long-bed gel permeation chromatography (gpc; sephadex lh-20) yielded purified flavonoids, with the most efficient minimum inhibitory concentrations (mics) against planktonic cells in the anthocyanin and procyanidin primary fractions against f. nucleatum (63-125 μg/ml) and in the procyanidin rich fraction against s. mutans (16-31 μg/ml). ... | 2014 | 24879903 |
| the kampo medicine rokumigan possesses antibiofilm, anti-inflammatory, and wound healing properties. | periodontal diseases, which are inflammatory diseases of bacterial origin affecting the tooth-supporting tissues, are characterized by inflammation and destruction of gingival connective tissue and alveolar bone, and may lead to tooth loss. the aim of the study was to investigate rokumigan, a kampo japanese traditional medicine made of six different plants, for its capacity to prevent biofilm formation by fusobacterium nucleatum, to inhibit interleukin-6 (il-6) and interleukin-8 (il-8) secretion ... | 2014 | 24877093 |
| microbial mucosal colonic shifts associated with the development of colorectal cancer reveal the presence of different bacterial and archaeal biomarkers. | epidemiological studies demonstrate a link between gastrointestinal cancers and environmental factors such as diet. it has been suggested that environmental cancer risk is determined by the interaction between diet and microbes. thus, the purpose of this study was to examine the hypothesis that microbiota composition during colorectal cancer (crc) progression might differ depending on the stage of the disease. | 2015 | 24811328 |
| periodontal disease, atherosclerosis, adverse pregnancy outcomes, and head-and-neck cancer. | interrelationships between periodontal infection and systemic conditions such as cardiovascular disease, adverse pregnancy outcomes, and head-and-neck cancer have become increasingly appreciated in recent years. periodontitis is associated with cardiovascular disease (cvd) and, experimentally, with measures of atherosclerosis and endothelial dysfunction. periodontal therapy may reduce atherosclerotic changes and improve endothelial function. preliminary findings suggest a role for the genetic lo ... | 2014 | 24736704 |
| microbial profile of root canals of primary teeth with pulp necrosis and periradicular lesion. | the purpose of this study was to assess the microbial content of root canals of human primary teeth with pulp necrosis and periradicular lesion. | 2014 | 24709428 |
| [effect of ultraviolet rays treatment of titanium and titanium alloy on antibacterial property]. | to evaluate the antibacterial property of ultraviolet rays (uv)-treated titanium and its alloy by observing the attachment and colony-forming units (cfu) of staphylococcus aureus (sa) and fusobacterium nucleatum (fn). | 2014 | 24697888 |
| oral cavity anaerobic pathogens in biofilm formation on voice prostheses. | a polymerase chain reaction (pcr)-based method has been used to identify oral anaerobic pathogens in biofilms on voice prostheses. the purpose of the present study was to determine the location of those pathogens inside the biofilms. | 2015 | 24677258 |
| signaling pathways activation by primary endodontic infectious contents and production of inflammatory mediators. | this study investigated the bacterial community involved in primary endodontic diseases, evaluated its ability to activate the macrophage toll-like receptor 4 receptor through p38 mitogen-activated protein kinase (mapk) and nuclear factor kappa b (nf-κb) signaling pathways, and determined the levels of endotoxins and interleukins (interleukin [il]-6 and -10) produced by endodontic content-stimulated macrophages. | 2014 | 24666896 |
| antimicrobial activities against periodontopathic bacteria of pittosporum tobira and its active compound. | the study of medicinal plants for treatment of periodontitis is of great value to establish their efficacy as sources of new antimicrobial drugs. five hundred and fifty eight korean local plant extracts were screened for antibacterial activity against representative periodontopathic bacteria such as porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum. among the various medicinal plants, the alcohol extract of pittosporum tobira, which significantly exhibited antibacteria ... | 2014 | 24662076 |
| evaluation of the sealing capability of implants to titanium and zirconia abutments against porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum under different screw torque values. | when evaluating long-term implant success, clinicians have always been concerned with the gap at the implant-abutment junction, where bacteria can accumulate and cause marginal bone loss. however, little information regarding bacterial leakage at the implant-abutment junction, or microgap, is available. | 2014 | 24656409 |
| acute appendicitis in children is associated with an abundance of bacteria from the phylum fusobacteria. | although luminal obstruction has traditionally been viewed as the underlying cause of appendicitis, recent evidence has suggested that the disease may result directly from invasion by specific pathogens, e.g. fusobacterium nucleatum. the purpose of this study was to survey microbial communities within pediatric appendectomy specimens using a culture-independent approach. | 2014 | 24650474 |
| evaluation of bacterial recovery and viability from three different swab transport systems. | this study evaluated three types of swab transport systems for organism recovery. swabs with transport media were further assessed for organism viability over 24 hours over a range of different storage temperatures. test organisms consisted of aerobes, fastidious aerobes and anaerobes. swabs were tested according to the standardised quantitative elution method published by the clinical laboratory standards institute (clsi; m-40a). there were substantial differences in primary organism recovery, ... | 2014 | 24614721 |
| preservation of fusobacterium nucleatum and peptostreptococcus anaerobius dna after loss of cell viability. | to investigate whether dna from two obligate anaerobes, fusobacterium nucleatum and peptostreptococcus anaerobius, is recoverable after loss of cell viability induced by air exposure. | 2015 | 24611977 |
| fusobacterium nucleatum associates with stages of colorectal neoplasia development, colorectal cancer and disease outcome. | commensal bacteria in the colon may play a role in colorectal cancer (crc) development. recent studies from north america showed that fusobacterium nucleatum (fn) infection is over-represented in disease tissue versus matched normal tissue in crc patients. using quantitative real-time polymerase chain reaction (qpcr) of dna extracted from colorectal tissue biopsies and surgical resections of three european cohorts totalling 122 crc patients, we found an over-abundance of fn in cancerous compared ... | 2014 | 24599709 |
| effect of estradiol on planktonic growth, coaggregation, and biofilm formation of the prevotella intermedia group bacteria. | alterations in the quantity and quality of biofilms at gingival margin are considered to play a role in the initiation and development of pregnancy-related gingivitis. prevotella intermedia sensu lato is able to consume estradiol, the major sex hormone secreted during pregnancy, in the absence of vitamin k. the aim of the study was to examine the effect of estradiol on the planktonic growth, coaggregation, polysaccharide production, and biofilm formation of the p. intermedia group bacteria, name ... | 2014 | 24594108 |
| lactobacillus salivarius wb21--containing tablets for the treatment of oral malodor: a double-blind, randomized, placebo-controlled crossover trial. | this study evaluated the effect of probiotic intervention using lactobacilli on oral malodor. | 2014 | 24556493 |
| genome sequence of fusobacterium nucleatum strain w1481, a possible new subspecies isolated from a periodontal pocket. | fusobacterium nucleatum is a bacterial species commonly detected in dental plaque within the human oral cavity, with some strains associated with periodontal disease, one of the most common clinical bacterial infections in the human body. the exact mechanisms of its pathogenesis are still not completely understood. in this study, we present the genome sequence and annotation of f. nucleatum strain w1481, isolated from a periodontal pocket of a dental patient at the university of bristol, united ... | 2014 | 24526626 |
| inhibition of glutamate racemase by substrate-product analogues. | d-glutamate is an essential biosynthetic building block of the peptidoglycans that encapsulate the bacterial cell wall. glutamate racemase catalyzes the reversible formation of d-glutamate from l-glutamate and, hence, the enzyme is a potential therapeutic target. we show that the novel cyclic substrate-product analogue (r,s)-1-hydroxy-1-oxo-4-amino-4-carboxyphosphorinane is a modest, partial noncompetitive inhibitor of glutamate racemase from fusobacterium nucleatum (fngr), a pathogen responsibl ... | 2014 | 24507924 |
| short-chain fatty acids from periodontal pathogens suppress histone deacetylases, ezh2, and suv39h1 to promote kaposi's sarcoma-associated herpesvirus replication. | periodontal pathogens such as porphyromonas gingivalis and fusobacterium nucleatum produce five different short-chain fatty acids (scfas) as metabolic by-products. we detect significantly higher levels of scfas in the saliva of patients with severe periodontal disease. the different scfas stimulate lytic gene expression of kaposi's sarcoma-associated herpesvirus (kshv) dose dependently and synergistically. scfas inhibit class-1/2 histone deacetylases (hdacs) and downregulate expression of silent ... | 2014 | 24501407 |
| inhibitory effects of sword bean extract on alveolar bone resorption induced in rats by porphyromonas gingivalis infection. | the domesticated legume, canavalia gladiata (commonly called the sword bean), is known to contain canavanine. the fruit is used in chinese and japanese herbal medicine for treating the discharge of pus, but its pharmacological mechanisms are still unclear. | 2014 | 24494651 |