| bacterial invasion into radicular dentine-an in vitro study. | we wanted to investigate differences in invasiveness into radicular dentinal tubules by monocultured and co-cultured bacteria frequently found in infected root canals. | 2016 | 27722787 |
| microbial dynamics during conversion from supragingival to subgingival biofilms in an in vitro model. | the development of dental caries and periodontal diseases result from distinct shifts in the microbiota of the tooth-associated biofilm. this in vitro study aimed to investigate changes in biofilm composition and structure, during the shift from a 'supragingival' aerobic profile to a 'subgingival' anaerobic profile. biofilms consisting of actinomyces oris, candida albicans, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar were aerobically grown in saliva ... | 2016 | 26033167 |
| axenic culture of a candidate division tm7 bacterium from the human oral cavity and biofilm interactions with other oral bacteria. | the diversity of bacterial species in the human oral cavity is well recognized, but a high proportion of them are presently uncultivable. candidate division tm7 bacteria are almost always detected in metagenomic studies but have not yet been cultivated. in this paper, we identified candidate division tm7 bacterial phylotypes in mature plaque samples from around orthodontic bonds in subjects undergoing orthodontic treatment. successive rounds of enrichment in laboratory media led to the isolation ... | 2014 | 25107981 |
| colonisation of gingival epithelia by subgingival biofilms in vitro: role of "red complex" bacteria. | biofilm formation on tooth surface results in colonisation and invasion of the juxtaposed gingival tissue, eliciting strong inflammatory responses that lead to periodontal disease. this in vitro study investigated the colonisation of human gingival multi-layered epithelium by multi-species subgingival biofilms, and evaluated the relative effects of the "red complex" species (porphyromonas gingivalis, tannerella forsythia and treponema denticola). | 2014 | 24949828 |
| red wine and oenological extracts display antimicrobial effects in an oral bacteria biofilm model. | the antimicrobial effects of red wine and its inherent components on oral microbiota were studied by using a 5-species biofilm model of the supragingival plaque that includes actinomyces oris, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar. microbiological analysis (cfu counting and confocal laser scanning microscopy) of the biofilms after the application of red wine, dealcoholized red wine, and red wine extract solutions spiked or not with grape seed ... | 2014 | 24773294 |
| strain-specific colonization patterns and serum modulation of multi-species oral biofilm development. | periodontitis results from an ecological shift in the composition of subgingival biofilms. subgingival community maturation is modulated by inter-organismal interactions and the relationship of communities with the host. in an effort to better understand this process, we evaluated biofilm formation, with oral commensal species, by three strains of the subgingivally prevalent microorganism fusobacterium nucleatum and four strains of the periodontopathogen porphyromonas gingivalis. we also tested ... | 2012 | 22771792 |
| [the efficacy of cold-light tooth bleaching on the formation of bacteria biofilm on the enamel surfaces]. | to evaluate the efficacy of beyond cold-light tooth bleaching on the formation of main cariogenic bacteria biofilm on enamel surfaces. | 2015 | 26166513 |
| influence of complement on neutrophil extracellular trap release induced by bacteria. | neutrophil extracellular trap (net) release has generally been studied in the absence of serum, or at low concentrations of untreated or heat-inactivated serum. the influence of serum complement on net release therefore remains unclear. we examined the dna release induced by staphylococcus aureus and three oral bacteria: actinomyces viscosus, aggregatibacter actinomycetemcomitans and fusobacterium nucleatum subsp. vincettii. | 2016 | 25900429 |
| influence of highly-active antiretroviral therapy on the subgingival biofilm in hiv-infected patients. | highly-active antiretroviral therapy (haart) has been associated with alterations in subgingival biofilm and periodontal disease. the purpose of the present study was to investigate the association between different haart regimens and the prevalence of periodontal pathogens in hiv-infected patients with chronic periodontitis. | 2016 | 25283543 |
| new bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings. | the aim of the present study was to analyze the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings. | 2014 | 24767562 |
| antibacterial activity of probiotic candidates for oral health. | the aim of this study was to determine the probiotic potential of autochthonous oral lactobacilli. for this, 66 strains were screened for antibacterial activity against two cariogenic strains (streptococcus mutans and actinomyces viscosus) and two periodontopathogenic strains (fusobacterium nucleatum and porphyromonas gingivalis). the inhibitory activity was investigated with the agar overlay technique. positive results led us to explore some mechanisms of action. the ability to produce h(2)o(2) ... | 2013 | 23211763 |
| evaluation of the bactericidal activity on oral organisms of the nd-yap laser in vitro. | the bactericidal activity of a pulsed fibre-optic nd-yap laser on various strains of oral bacteria: prevotella nigrescens, streptococcus sanguis, actinomyces viscosus (i), fusobacterium nucleatum, actinobacillus actinomycetemcomitans and peptostreptococcus micros (i) was evaluated. a 200-μm optical fibre was placed in eppendorf tubes containing the different bacterial suspensions. at an energy of 170 mj/30 hz, 351 pulses of 150 μs were sufficient to kill the most resistant bacteria (i). the expo ... | 1998 | 24710990 |
| term and preterm labour are associated with distinct microbial community structures in placental membranes which are independent of mode of delivery. | infection is considered a possible trigger for preterm labour, supported by evidence showing the presence of bacteria in the placenta and placental membranes from preterm births. in this study, 16s rdna pyrosequencing was used to identify bacteria in placental membranes. caesarean sections and vaginal deliveries at term were found to harbour common genera. mycoplasma hominis, aerococcus christensenii, gardnerella vaginalis and fusobacterium nucleatum were either only present in preterm membranes ... | 2014 | 25458966 |
| subgingival microbiota in caucasian patients with desquamative gingivitis: a cross-sectional study. | the presence of epithelial desquamation, erythema, and erosions on the gingival tissue is usually described in literature as desquamative gingivitis (dg). a wide range of autoimmune/dermatological disorders can manifest as dg, although the two more common are oral lichen planus and mucous membrane pemphigoid. the aim of this study was to investigate the prevalence of 11 periodonto-pathogenic microorganisms in dg cases and to compare it with microbiologic status of individuals affected by plaque- ... | 2017 | 28304209 |
| response to antiseptic agents of periodontal pathogens in in vitro biofilms on titanium and zirconium surfaces. | the aim of this study was to develop in vitro biofilms on sla titanium (ti-sla) and zirconium oxide (zro2) surfaces and to evaluate the effect of antiseptic agents on the number of putative periodontal pathogenic species. | 2017 | 28237092 |
| effect of opposing implant prostheses on periodontal pathogens in dentures: a pilot study. | an understanding of the presence of periodontal pathogens in denture plaque is important for the treatment of patients with edentulism. however, current data are limited and inconclusive. | 2017 | 28126355 |
| rt-pcr quantification of periodontal pathogens in crack users and non-users. | to compare counts of aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis and fusobacterium nucleatum between crack users and non-users. | 2017 | 27982490 |
| high-risk periodontal pathogens contribute to the pathogenesis of atherosclerosis. | periodontal disease (pd) is generated by microorganisms. these microbes can enter the general circulation causing a bacteraemia. the result can be adverse systemic effects, which could promote conditions such as cardiovascular disease. level a evidence supports that pd is independently associated with arterial disease. pd is a common chronic condition affecting the majority of americans 30 years of age and older. atherosclerosis remains the largest cause of death and disability. studies indicate ... | 2017 | 27899684 |
| thermoreversible gel for intrapocket delivery of green tea catechin as a local drug delivery system: an original research. | the periodontal therapies along with systemic antibiotic therapy aim at eliminating the subgingival microbiota to arrest the progression of periodontal diseases. the complete elimination is often difficult, and thus the probability of repopulation after periodontal therapy is also high. the objectives of the study are to develop in situ thermoreversible gelling system of green tea catechins suitable for periodontal pocket administration, which would act as an adjunct to mechanical periodontal th ... | 2017 | 27833893 |
| changes in salivary periodontal pathogens after orthodontic treatment: an in vivo prospective study. | to analyze the initial changes in salivary levels of periodontal pathogens after orthodontic treatment with fixed appliances. | 2016 | 27792427 |
| comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample? | the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control). | 2017 | 27786617 |
| does obesity influence the subgingival microbiota composition in periodontal health and disease? | to evaluate whether obesity affects the subgingival microbial composition of patients with periodontal health or chronic periodontitis (cp). | 2016 | 27717180 |
| effect of bioactive dental adhesive on periodontal and endodontic pathogens. | the objectives of this study were to: (1) develop a new bioactive dental bonding agent with nanoparticles of amorphous calcium phosphate and dimethylaminohexadecyl methacrylate for tooth root caries restorations and endodontic applications, and (2) investigate biofilm inhibition by the bioactive bonding agent against eight species of periodontal and endodontic pathogens for the first time. bonding agent was formulated with 5 % of dimethylaminohexadecyl methacrylate. nanoparticles of amorphous ca ... | 2016 | 27699574 |
| novel bioactive nanocomposite for class-v restorations to inhibit periodontitis-related pathogens. | the occurrence of tooth root caries is increasing as the world population ages and tooth retention in seniors increases. class v restorations with subgingival margins are difficult to clean and often lead to periodontitis. the objectives of this study were to develop a class v composite containing dimethylaminohexadecyl methacrylate (dmahdm) and nanoparticles of amorphous calcium phosphate (nacp), and investigate mechanical properties and the inhibition of six species of periodontitis-related bi ... | 2016 | 27671471 |
| microbial characteristics of peri-implantitis: a case-control study. | the aim of this case-control study is to compare oral microbiologic characteristics of patients with healthy peri-implant conditions and patients with peri-implantitis and to explore the influence of various patient- and implant-related factors on microbiologic characteristics. | 2017 | 27666672 |
| effect of helicobacter pylori infection on chronic periodontitis by the change of microecology and inflammation. | helicobacter pylori (h. pylori), a pathogen inducing peptic disease, is recently found to be binding to the progress of periodontitis. most previous studies are case-controlled, and they investigate the risk of h. pylori infection in disease the development of while few studies evaluate the correlation between h. pylori and periodontal pathogens. therefore, we investigated the correlation between h. pylori infection with periodontal parameters, periodontal pathogens and inflammation. the results ... | 2016 | 27602578 |
| adhesion of periodontal pathogens to self-ligating orthodontic brackets: an in-vivo prospective study. | our aims were to analyze adhesion of periodontopathogens to self-ligating brackets (clarity-sl [csl], clippy-c [cc] and damon q [dq]) and to identify the relationships between bacterial adhesion and oral hygiene indexes. | 2016 | 27585775 |
| novel bioactive tetracycline-containing electrospun polymer fibers as a potential antibacterial dental implant coating. | the purpose of this investigation was to determine the ability of tetracycline-containing fibers to inhibit biofilm formation of peri-implantitis-associated pathogens [i.e., porphyromonas gingivalis (pg), fusobacterium nucleatum (fn), prevotella intermedia (pi), and aggregatibacter actinomycetemcomitans (aa)]. tetracycline hydrochloride (tch) was added to a poly(dl-lactide) [pla], poly(ε-caprolactone) [pcl], and gelatin [gel] polymer blend solution at distinct concentrations to obtain the follow ... | 2016 | 27585669 |
| d-galactose as an autoinducer 2 inhibitor to control the biofilm formation of periodontopathogens. | autoinducer 2 (ai-2) is a quorum sensing molecule to which bacteria respond to regulate various phenotypes, including virulence and biofilm formation. ai-2 plays an important role in the formation of a subgingival biofilm composed mostly of gram-negative anaerobes, by which periodontitis is initiated. the aim of this study was to evaluate d-galactose as an inhibitor of ai-2 activity and thus of the biofilm formation of periodontopathogens. in a search for an ai-2 receptor of fusobacterium nuclea ... | 2016 | 27572513 |
| longitudinal study on clinical and microbial analysis of periodontal status in pregnancy. | this study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (fish) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. probing depth (pd), bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque sampl ... | 2016 | 27556678 |
| detection of selected periodontal bacteria in preschool children affected by early childhood caries. | the aim of this study was to compare the detection frequency of periodontal bacteria in dental plaque in children with early childhood caries (ecc) with and without gingival inflammation. a convenience sample of 25 preschool children (mean age 3.61 years, sd 1.42) was recruited. dental plaque was taken from periodontal areas with and without visible signs of inflammation and processed using the stomagene® (protean s.r.o. czech republic) and parocheck® 20 (greiner bio-one gmbh, germany) detection ... | 2016 | 27485551 |
| nucleases from prevotella intermedia can degrade neutrophil extracellular traps. | periodontitis is an inflammatory disease caused by periodontal bacteria in subgingival plaque. these bacteria are able to colonize the periodontal region by evading the host immune response. neutrophils, the host's first line of defense against infection, use various strategies to kill invading pathogens, including neutrophil extracellular traps (nets). these are extracellular net-like fibers comprising dna and antimicrobial components such as histones, ll-37, defensins, myeloperoxidase, and neu ... | 2016 | 27476978 |
| an assessment of early colonisation of implant-abutment metal surfaces by single species and co-cultured bacterial periodontal pathogens. | numerous studies have proposed that smooth metal surfaces reduce initial bacterial attachment in the establishment of an early biofilm formation. however, these studies have largely examined single bacterial species, which are not always relevant as pathogens identified as initiators of inflammatory peri-implantitis. this study investigated the adherence of four periodontally-relevant bacterial species to implant and abutment surfaces in current clinical use. | 2016 | 27472955 |
| bacterial load of periodontal pathogens among italian patients with chronic periodontitis: a comparative study of three different areas. | the aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in italian patients affected by chronic periodontitis. the sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology sampled in the period 2013-2015; 1,323 patients were from northern italy, 317 from central italy and 122 from southern italy. samples for microbiological analysis were collected from ... | 2016 | 27469562 |
| evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using pcr analysis: a microbiological pilot study. | the use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. over the last few years, carvacrol and thymol (ct) have been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of ct on the red complex bacteria using polymerase chain reaction (pcr) for microbiological analysis. five patients with a diagnosis of chronic periodontitis in the age grou ... | 2016 | 27469559 |
| interactions between lactobacillus rhamnosus gg and oral micro-organisms in an in vitro biofilm model. | probiotics have shown favourable properties in maintaining oral health. by interacting with oral microbial communities, these species could contribute to healthier microbial equilibrium. this study aimed to investigate in vitro the ability of probiotic lactobacillus rhamnosus gg (l.gg) to integrate in oral biofilm and affect its species composition. five oral strains, streptococcus mutans, streptococcus sanguinis, aggregatibacter actinomycetemcomitans, fusobacterium nucleatum and candida albican ... | 2016 | 27405227 |
| a protein-repellent and antibacterial nanocomposite for class-v restorations to inhibit periodontitis-related pathogens. | the objectives of this study were to develop a bioactive dental composite and investigate the effects of 2-methacryloyloxyethyl phosphorylcholine (mpc) and dimethylaminohexadecyl methacrylate (dmahdm) in class v composite on mechanical properties, water sorption, protein adsorption, and inhibition of four species of periodontitis-related biofilms for the first time. the resin consisted of ethoxylated bisphenol a dimethacrylate (ebpadma) and pyromellitic glycerol dimethacrylate (pmgdm). dmahdm, m ... | 2016 | 27287170 |
| development of novel electrospun dual-drug fiber mats loaded with a combination of ampicillin and metronidazole. | our study was performed with the aim of preparing electrospun polylactide fibers with a combination of ampicillin (amp) and metronidazole (mnz) and investigating their drug release behavior and the antibacterial effect on aggregatibacter actinomycetemcomitans and other oral pathogens. | 2016 | 27262200 |
| withaferin a inhibits inflammatory responses induced by fusobacterium nucleatum and aggregatibacter actinomycetemcomitans in macrophages. | periodontitis is a progressive chronic inflammatory disease and a major cause of tooth loss in humans. as a withanolides, withaferin a (wa) is known to exhibit strong anti‑inflammatory activity. the present study examined whether wa inhibited inflammatory responses in macrophages in response to two representative periodontal pathogens, fusobacterium nucleatum and aggregatibacter actinomycetemcomitans. murine bone marrow‑derived macrophages (bmdms) were used in this study and cytokine production ... | 2016 | 27220676 |
| detection of five potentially periodontal pathogenic bacteria in peri-implant disease: a comparison of pcr and real-time pcr. | the aim of this study was to compare the microbial analysis methods of polymerase chain reaction (pcr) and real-time pcr (rt-pcr) in terms of detection of five selected potentially periodontal pathogenic bacteria in peri-implant disease. therefore 45 samples of healthy, mucositis and peri-implantitis (n = 15 each) were assessed according to presence of the following bacteria using pcr (dna-strip technology) and rt-pcr (fluorescent dye sybr green-system): aggregatibacter actinomycetemcomitans (aa ... | 2016 | 27142589 |
| antibacterial effect of silver (i) carbohydrate complexes on oral pathogenic key species in vitro. | it was the aim of this study to evaluate the antibacterial impact of two silver(i) carbohydrate complexes with tripodal thioglycosides, namely tris[2-(β-d-thio-glucopyranosyl)ethyl]-amine-silver(i)-nitrate (3) and tris[2-(α-d-thio-manno-pyranosyl)ethyl]-amine-silver(i)-nitrate (4), on five oral pathogenic bacterial strains. furthermore, cytocompatibility was tested using human gingival fibroblasts (hgf). | 2016 | 27009305 |
| influence of a triclosan toothpaste on periodontopathic bacteria and periodontitis progression in cardiovascular patients: a randomized controlled trial. | triclosan/copolymer toothpaste is effective in controlling plaque and gingivitis and in slowing the progression of periodontitis. this study describes its influence on microbiological and clinical outcomes, over a 5-year period, in patients with established cardiovascular disease (cvd). | 2017 | 26932733 |
| early colonization of the oral cavity in 6- and 12-month-old infants by cariogenic and periodontal pathogens: a case-control study. | the colonization of the oral cavity by cariogenic and periodontal pathogens occurs earlier than previously thought. this study aimed to identify the presence and quantity of representative cariogenic and periodontal pathogens in the oral cavities of 6- and 12-month olds and to evaluate the influence of c-section delivery on early streptococcus mutans (sm) colonization of the oral cavity. the research cohort was composed of 59 infants (35 infants were delivered vaginally and 24 via c-section) and ... | 2016 | 26914065 |
| microbiological distribution of six periodontal pathogens between untreated italian and dutch periodontal patients. | to compare prevalence and microbial load of six periodontal pathogens between italian and dutch patients affected by chronic periodontitis, using oligonucleotide probe technology. | 2016 | 26870849 |
| effect of low-level laser therapy as an adjuvant in the treatment of periodontitis induced in rats subjected to 5-fluorouracil chemotherapy. | this study aimed to assess the effect of multiple sessions of a low-level laser therapy (lllt) adjuvant to scaling and root planing (srp) on the treatment of experimental periodontitis (ep) in rats treated with 5-fluorouracil (5-fu). | 2016 | 26750739 |
| scaling and root planning, and locally delivered minocycline reduces the load of prevotella intermedia in an interdependent pattern, correlating with symptomatic improvements of chronic periodontitis: a short-term randomized clinical trial. | to evaluate the respective or combinatory efficacy of locally delivered 2% minocycline (mo), and scaling and root planning (srp) by assessing both clinical parameters and the loads of four main periodontal pathogens in treating chronic periodontitis (cp). | 2015 | 26676022 |
| changes in salivary periodontal pathogens after orthodontic treatment: an in vivo prospective study. | to analyze the initial changes in salivary levels of periodontal pathogens after orthodontic treatment with fixed appliances. | 2016 | 26606331 |
| the in vitro antimicrobial efficacy of pdt against periodontopathogenic bacteria. | periodontitis, an inflammatory disease, is caused by biofilms with a mixed microbial etiology and involves the progressive destruction of the tooth-supporting tissues. a rising number of studies investigate the clinical potential of photodynamic therapy (pdt) as an adjunct during active therapy. the aim of the present review was to evaluate the available literature for the in vitro antimicrobial efficacy of photodynamic therapy focusing on the periodontopathogenic bacteria aggregatibacter actino ... | 2015 | 26580607 |
| secreted adenosine triphosphate from aggregatibacter actinomycetemcomitans triggers chemokine response. | extracellular atp (eatp) is an important intercellular signaling molecule secreted by activated immune cells or released by damaged cells. in mammalian cells, a rapid increase of atp concentration in the extracellular space sends a danger signal, which alerts the immune system of an impending danger, resulting in recruitment and priming of phagocytes. recent studies show that bacteria also release atp into the extracellular milieu, suggesting a potential role for eatp in host-microbe interaction ... | 2016 | 26470857 |
| oral microbiota, periodontal status, and osteoporosis in postmenopausal females. | alterations in hormone levels during menopause decrease bone density and may worsen oral health, favoring the growth of periodontal pathogens, whose detection could improve the diagnosis of periodontitis. the aim of this study is to detect and quantify the main periodontal pathogens in the oral microbiota of postmenopausal females and to explore the relationship between clinical and periodontal parameters. | 2016 | 26469811 |
| antibacterial activity of curcumin against periodontopathic bacteria. | curcumin is a polyphenol extracted from root of turmeric and known to possess multifunctional properties, including antibacterial activity. although previous studies have investigated the effects of curcumin on microorganisms, available knowledge on the effects of curcumin on periodontopathic bacteria is still limited. in this study, the antibacterial effect of curcumin on periodontopathic bacteria is investigated, particularly porphyromonas gingivalis. | 2016 | 26447754 |
| evaluation of the antibacterial effect of silver nanoparticles on guided tissue regeneration membrane colonization--an in vitro study. | the aim of this in vitro study was to investigate the colonization and penetration of specific bacteria on nanosilver-impregnated gtr (guided tissue regeneration) membranes. | 2015 | 26373223 |
| significant increase of oral bacteria in the early pregnancy period in japanese women. | oral microflora during pregnancy is critical to oral health care in the mother and her child. we examined the changes in the oral microbiota between pregnancy and nonpregnancy periods. | 2017 | 26345599 |
| microbiologic and clinical findings of implants in healthy condition and with peri-implantitis. | to compare implants in healthy conditions and implants with peri-implantitis with regard to their clinical parameters and the microbiologic composition at the peri-implant sulcus, inside the implant connection, and the gingival sulcus of neighboring teeth. | 2016 | 26252036 |
| detection of subgingival periodontal pathogens--comparison of two sampling strategies. | the aim of the study is to compare detection frequency of periodontal pathogens in patients with aggressive/severe chronic periodontitis using pooled plaque samples from the deepest pockets per quadrant/per sextant. | 2016 | 26193958 |
| coinfection with fusobacterium nucleatum can enhance the attachment and invasion of porphyromonas gingivalis or aggregatibacter actinomycetemcomitans to human gingival epithelial cells. | this study was conducted to investigate effects of coinfection of porphyromonas gingivalis (p. gingivalis) or aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans) with fusobacterium nucleatum (f. nucleatum) on their adhering and invasive capacity to human gingival epithelial cells as well as the expression of interleukin-8 (il-8) and human beta-defensin-2 (hbd-2) in human gingival epithelial cells. | 2015 | 26143497 |
| determination of antibacterial activity of green coffee bean extract on periodontogenic bacteria like porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum and aggregatibacter actinomycetemcomitans: an in vitro study. | the aim of this study was to evaluate the antibacterial activity of pure green coffee bean extract on periodonto pathogenic bacteria porphyromonas gingivalis (pg), prevotella intermedia (pi), fusobacterium nucleatum (fn) and aggregatibacter actinomycetemcomitans (aa). | 2017 | 26097349 |
| alcohol consumption and periodontitis: quantification of periodontal pathogens and cytokines. | there are few studies on periodontal status related to microbiologic and immunologic profiles among individuals not or occasionally using alcohol and those with alcohol dependence. the aim of this study is to determine the effect of alcohol consumption on the levels of subgingival periodontal pathogens and proinflammatory cytokines (interleukin [il]-1β and tumor necrosis factor [tnf]-α) in the gingival fluid among individuals with and without periodontitis. | 2015 | 26062839 |
| quantitation of biofilm and planktonic life forms of coexisting periodontal species. | complexity of oral polymicrobial communities has prompted a need for developing in vitro models to study behavior of coexisting bacteria. little knowledge is available of in vitro co-growth of several periodontitis-associated species without early colonizers of dental plaque. | 2015 | 25926392 |
| antimicrobial activity of chemokine cxcl10 for dermal and oral microorganisms. | cxcl10 (ip-10) is a small 10 kda chemokine with antimicrobial activity. it is induced by ifn-γ, chemoattracts mononuclear cells, and promotes adhesion of t cells. recently, we detected cxcl10 on the surface of the skin and in the oral cavity. in the current study, we used broth microdilution and radial diffusion assays to show that cxcl10 inhibits the growth of escherichia coli, staphylococcus aureus, corynebacterium jeikeium, corynebacterium striatum, and candida albicans hmv4c, but not coryneb ... | 2014 | 25859394 |
| occurrence of periodontal pathogens in ethnic groups from a native brazilian reservation. | the present study was designed to evaluate the occurrence of periodontal pathogens in the subgingival biofilm of 100 native brazilians living at the umutina indian reservation, mato grosso state, brazil. | 2015 | 25847068 |
| antibacterial photodynamic treatment of periodontopathogenic bacteria with indocyanine green and near-infrared laser light enhanced by trolox(tm). | it has been shown that certain vitamins can significantly enhance the effect of photodynamic anti-tumor therapy. unfortunately, there is no sufficient information available about the impact of those antioxidants on antimicrobial photodynamic therapy (apdt). the present study is aimed at investigating the antimicrobial effect of the dye indocyanine green (icg) in the presence of trolox(tm) , a vitamin e analogue, upon irradiation with near-infrared (nir) laser light (808 nm) on the gramnegative p ... | 2015 | 25753989 |
| anti-inflammatory, antioxidant and antimicrobial effects of artemisinin extracts from artemisia annua l. | the anti-inflammatory, antioxidant, and antimicrobial properties of artemisinin derived from water, methanol, ethanol, or acetone extracts of artemisia annua l. were evaluated. all 4 artemisinin-containing extracts had anti-inflammatory effects. of these, the acetone extract had the greatest inhibitory effect on lipopolysaccharide-induced nitric oxide (no), prostaglandin e2 (pge2), and proinflammatory cytokine (il-1β , il-6, and il-10) production. antioxidant activity evaluations revealed that t ... | 2015 | 25605993 |
| selected dietary (poly)phenols inhibit periodontal pathogen growth and biofilm formation. | periodontitis (pd) is a chronic infectious disease mediated by bacteria in the oral cavity. (poly)phenols (pps), ubiquitous in plant foods, possess antimicrobial activities and may be useful in the prevention and management of periodontitis. the objective of this study was to test the antibacterial effects of selected pps on periodontal pathogens, on both planktonic and biofilm modes of growth. selected pps (n = 48) were screened against streptococcus mitis (s. mitis), aggregatibacter actinomyce ... | 2015 | 25585200 |
| microbial leakage at the implant-abutment connection due to implant insertion maneuvers: cross-sectional study 5 years postloading in healthy patients. | the aim of this study was to test if stress on the prosthetic connection during insertion maneuvers can induce micro-warping at the implant connection. from september 2011 to july 2013, patients with implants loaded for at least 5 years that were placed with 2 different insertion implant mounters-mp (conventional) and me (mountless)-were selected from all of those who had received dental implant therapy in the past and were attending routine check-up or spontaneous visits during the study period ... | 2015 | 25536224 |
| distribution of 8 periodontal microorganisms in family members of chinese patients with aggressive periodontitis. | to date, no information on the distribution of periodontal microorganisms among family members of chinese patients with aggressive peridontitis (agp) is available. the aim of the present study was to investigate the probability of transmission of eight periodontal microorganisms between patients with aggressive periodontitis and their family members. | 2015 | 25526624 |
| regulation of adrenomedullin and nitric oxide production by periodontal bacteria. | in periodontitis the host response to bacterial challenge includes activity of the multifunctional molecules adrenomedullin (am) and nitric oxide (no). the aim of this study was to investigate the role of periodontal bacteria in regulating the production of these molecules from cultured cells. | 2015 | 25440112 |
| detection of periodontal pathogens in the patients with aortic aneurysm. | the occurrence and development of aortic aneurysm (aa) are associated with infection. some researchers have detected the dna of periodontal pathogens in aa samples in certain populations. however, it has not been done in chinese population. the objective of this study was to evaluate the prevalence of periodontal pathogens in oral tissue samples and aneurysm samples of aa patients. | 2014 | 25430459 |
| periodontal and peri-implant microbiota in patients with healthy and inflamed periodontal and peri-implant tissues. | to compare the prevalence and levels of six bacterial pathogens within the subgingival/submucosal microbiota at teeth versus implants with various clinical conditions. | 2016 | 25399962 |
| prevalence of six periodontal pathogens in subgingival samples of italian patients with chronic periodontitis. | the aim of this study was to investigate the subgingival prevalence of six periodontal pathogens in 352 italian patients with chronic periodontitis. possible correlations with clinical parameters, age, gender and smoking status were also investigated. at first visit a pooled subgingival plaque sample was obtained for each subject by using the paper-point method. the samples were processed and analysed according to a commercially available quantitative real time polymerase chain reaction assay (m ... | 2014 | 25387289 |
| essential oils and isolated compounds from lippia alba leaves and flowers: antimicrobial activity and osteoclast apoptosis. | in the present study, essential oils extracted from the leaves and flowers of lippia alba (mill.) n.e.br. (l. alba) were analyzed for their antimicrobial activity and their effects on osteoclasts. the periodontal pathogens, aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans; atcc 43717), fusobacterium nucleatum (f. nucleatum; atcc 25586) and porphyromonas gingivalis (p. gingivalis); atcc 33277) were used in antimicrobial activity assays for determining the minimum inhibitory concent ... | 2015 | 25384405 |
| antimicrobial activity of platelet-rich plasma and other plasma preparations against periodontal pathogens. | in addition to releasing a pool of growth factors during activation, platelets have many features that indicate their role in the anti-infective host defense. the antimicrobial activities of platelet-rich plasma (prp) and related plasma preparations against periodontal disease-associated bacteria were evaluated. | 2015 | 25345340 |
| effect of non-surgical periodontal treatment on short chain fatty acid levels in gingival crevicular fluid of patients with generalized aggressive periodontitis. | short chain fatty acids (scfas) play important roles in periodontal diseases. however, the concentrations of scfas in gingival crevicular fluid of patients with aggressive periodontitis are not known. the aim of this intervention study was to investigate the influences of non-surgical periodontal therapy on levels of scfas in the gingival crevicular fluid of patients with generalized aggressive periodontitis (g-agp), and analyze the concentrations of scfas in sites with or without the detected p ... | 2014 | 25340203 |
| effect of bdellovibrio bacteriovorus hd100 on multispecies oral communities. | the predation of bdellovibrio bacteriovorus on different periodontal pathogens has already been described. however, it is necessary to consider the polymicrobial nature of periodontal disease. the current study explores the predation of bdellovibrio on oral pathogens organized in multispecies communities. the effect of the predator was evaluated on in vitro six species communities with microbial culturing. additionally, the effect on ex vivo subgingival plaque and saliva samples from periodontit ... | 2015 | 25252124 |
| real-time pcr quantification of six periodontal pathogens in saliva samples from healthy young adults. | the use of saliva as a diagnostic fluid for the evaluation of periodontal health has gained attention recently. most published real-time pcr assays focused on quantification of bacteria in subgingival plaque, not in saliva. the aims of this study were to develop a real-time pcr assay for quantification of six periodontal pathogens in saliva and to establish a relationship between the amount of dna (fg) and colony-forming unit (cfu). | 2015 | 25217278 |
| nanoparticle-encapsulated chlorhexidine against oral bacterial biofilms. | chlorhexidine (chx) is a widely used antimicrobial agent in dentistry. herein, we report the synthesis of a novel mesoporous silica nanoparticle-encapsulated pure chx (nano-chx), and its mechanical profile and antimicrobial properties against oral biofilms. | 2014 | 25170958 |
| the interaction of implant luting cements and oral bacteria linked to peri-implant disease: an in vitro analysis of planktonic and biofilm growth--a preliminary study. | there is little consensus on the most appropriate cement to use when restoring a cement-retained, implant-supported restoration. one consideration should be the interaction of pathogenic oral bacteria with restorative cements. | 2015 | 24909337 |
| internal bacterial colonization of implants: association with peri-implant bone loss. | the aim of the present longitudinal study was to investigate bacterial colonization of the internal implant cavity and to evaluate a possible association with peri-implant bone loss. | 2015 | 24861845 |
| clinical and microbiological characterization of localized aggressive periodontitis: a cohort study. | localized aggressive periodontitis (lagp) is an infectious periodontal disease which generally affects young people. recent data suggest the involvement of different bacterial species in different populations. the causative bacterial species in israel has never been identified despite a high prevalence of lagp in this population. the objectives of this study were to characterize the bacterial microbiota of periodontal pockets within an israeli lagp population who were also clinically assessed. | 2014 | 24861390 |
| evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time pcr. | the aim of this study was to investigate the mandibular third molar pericoronitis flora by using real-time polymerase chain reaction (pcr). | 2014 | 24835305 |
| relationship between expression of human gingival beta-defensins and levels of periodontopathogens in subgingival plaque. | human beta-defensins (hbds) are a group of antimicrobial peptides important in epithelial innate immunity, and their differential expression is associated with periodontal diseases. the aim of this study was to explore relationships among hbds, total subgingival bacteria and periodontopathogens in healthy subjects and in patients with chronic periodontitis. | 2015 | 24814979 |
| antibacterial action of chlorhexidine/thymol containing varnishes in vitro and in vivo. | the antibacterial activity of two different formulations of a chlorhexidine/thymol varnish should be elucidated in vitro and in vivo. | 2014 | 24738759 |
| combinatorial effects of amoxicillin and metronidazole on selected periodontal bacteria and whole plaque samples. | the aim of the present study was to analyze in vitro the combinatorial effects of the antibiotic combination of amoxicillin plus metronidazole on subgingival bacterial isolates. | 2014 | 24727003 |
| microbiological assessment of the implant-abutment interface in different connections: cross-sectional study after 5 years of functional loading. | to evaluate the bacterial microflora present inside the implant connection and in the peri-implant sulcus fluid of healthy implants, and to analyze the relationships between these harboring sites for four different implant systems after at least 5 years of functional loading. | 2015 | 24665919 |
| diverse toll-like receptors mediate cytokine production by fusobacterium nucleatum and aggregatibacter actinomycetemcomitans in macrophages. | toll-like receptors (tlrs) orchestrate a repertoire of immune responses in macrophages against various pathogens. fusobacterium nucleatum and aggregatibacter actinomycetemcomitans are two important periodontal pathogens. in the present study, we investigated tlr signaling regulating cytokine production of macrophages in response to f. nucleatum and a. actinomycetemcomitans. tlr2 and tlr4 are redundant in the production of cytokines (interleukin-6 [il-6] and tumor necrosis factor alpha [tnf-α]) i ... | 2014 | 24566622 |
| biodegradable nanofibrous drug delivery systems: effects of metronidazole and ciprofloxacin on periodontopathogens and commensal oral bacteria. | the purposes of this study were to fabricate biodegradable polydioxanone (pds ii®) electrospun periodontal drug delivery systems (hereafter referred to as matrices) containing either metronidazole (met) or ciprofloxacin (cip) and to investigate the effects of antibiotic incorporation on both periodontopathogens and commensal oral bacteria. | 2014 | 24535074 |
| photodynamic antimicrobial effect of safranine o on an ex vivo periodontal biofilm. | the increasing resistance of oral pathogens against antibiotic measures urgently requires new therapeutic strategies. in this context, antimicrobial photodynamic therapy (apdt) may play a crucial part in the future. the aim of the present study was to compare the antibacterial efficiency of apdt using the photosensitizer safranine o with that of chlorhexidine (0.2% chx) on an ex vivo biofilm. | 2014 | 24473989 |
| periodontal bacteria in the genital tract: are they related to adverse pregnancy outcome? | one of the most important factors implicated in preterm birth (ptb) is acute genitourinary tract infection. the bacteria causing chronic periodontal inflammation include gram-negative rods and anaerobes similar to those found in women with bacterial vaginosis. the aim of this prospective study is to investigate the relationship between oral and vaginal microflora and preterm low birth weight. real-time polymerase chain reaction was used to detect both the presence and level of six periodontitis- ... | 2017 | 24355228 |
| anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system. | human mesenchymal stem cells (hmscs) are multipotent by nature and are originally isolated from bone marrow. in light of a future application of hmscs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacterial species i.e. periodontitis pathogens, we performed this study to gain information about the behavior of hmsc in an anaerobic system and the response in interaction with oral bacterial pathogens. | 2013 | 24223777 |
| antimicrobial photodynamic efficiency of novel cationic porphyrins towards periodontal gram-positive and gram-negative pathogenic bacteria. | the gram-negative aggregatibacter actinomycetemcomitans and fusobacterium nucleatum are major causative agents of aggressive periodontal disease. due to increase in the number of antibiotic-resistant bacteria, antimicrobial photodynamic therapy (apdt) seems to be a plausible alternative. in this work, photosensitization was performed on gram-positive and gram-negative bacteria in pure culture using new-age cationic porphyrins, namely mesoimidazolium-substituted porphyrin derivative (imp) and pyr ... | 2014 | 24164211 |
| oral community interactions of filifactor alocis in vitro. | filifactor alocis is a gram positive anaerobe that is emerging as an important periodontal pathogen. in the oral cavity f. alocis colonizes polymicrobial biofilm communities; however, little is known regarding the nature of the interactions between f. alocis and other oral biofilm bacteria. here we investigate the community interactions of two strains of f. alocis with streptococcus gordonii, fusobacterium nucleatum, porphyromonas gingivalis and aggregatibacter actinomycetemcomitans, organisms w ... | 2013 | 24098460 |
| antimicrobial activity of isothiocyanates (itcs) extracted from horseradish (armoracia rusticana) root against oral microorganisms. | the antimicrobial activity of isothiocyanates (itcs) extracted from horseradish root was investigated against oral microorganisms: 6 strains of facultative anaerobic bacteria, streptococcus mutans, streptococcus sobrinus, lactobacillus casei, staphylococcus aureus, enterococcus faecalis and aggregatibacter actinomycetemcomitans; one strain of yeast, candida albicans, and 3 strains of anaerobic bacteria, fusobacterium nucleatum, prevotella nigrescens, and clostridium perfringens. the itcs extract ... | 2013 | 24077540 |
| salivary detection of periodontopathic bacteria in fanconi's anemia patients. | fanconi's anemia (fa) is characterized by bone marrow failure and can lead to infections such as periodontal disease. the aim of this study was to compare the prevalence of four periodontopathic bacteria including aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum and treponema denticola in saliva samples from children with and without fa. paraffin-stimulated saliva samples were collected from 71 children and adolescents, aged 6-18 years old. the samples wer ... | 2013 | 24055631 |
| the connection between ruptured cerebral aneurysms and odontogenic bacteria. | patients with ruptured saccular intracranial aneurysms have excess long-term mortality due to cerebrovascular and cardiovascular diseases compared with general population. chronic inflammation is detected in ruptured intracranial aneurysms, abdominal aortic aneurysms and coronary artery plaques. bacterial infections have been suggested to have a role in the aetiology of atherosclerosis. bacteria have been detected both in abdominal and coronary arteries but their presence in intracranial aneurys ... | 2013 | 23761916 |
| porphyromonas gingivalis, treponema denticola and toll-like receptor 2 are associated with hypertensive disorders in placental tissue: a case-control study. | to explore the associations between the presence of periodontal pathogens and the expression of toll-like receptors (tlr-2 and tlr-4) in the placental tissue of patients with hypertensive disorders compared to the placentas of healthy normotensive patients. | 2013 | 23711357 |
| phototoxic effect of blue light on the planktonic and biofilm state of anaerobic periodontal pathogens. | the purpose of this study was to compare the phototoxic effects of blue light exposure on periodontal pathogens in both planktonic and biofilm cultures. | 2013 | 23678390 |
| detection of eight periodontal microorganisms and distribution of porphyromonas gingivalis fima genotypes in chinese patients with aggressive periodontitis. | the microbiologic feature of aggressive periodontitis (agp) in chinese patients has not yet been determined. this study aims to investigate the prevalence of eight periodontal microorganisms and the distribution of the porphyromonas gingivalis fima genotype in a cohort of chinese patients with agp. | 2014 | 23646850 |
| comparative evaluation of subgingival plaque microflora in pregnant and non-pregnant women: a clinical and microbiologic study. | gingival changes in pregnancy have been attributed to changes in the subgingival biofilm related to hormonal variations. | 2013 | 23633772 |
| quantitative real-time pcr combined with propidium monoazide for the selective quantification of viable periodontal pathogens in an in vitro subgingival biofilm model. | differentiation of live and dead cells is an important challenge when using molecular diagnosis for microbial identification. this is particularly relevant when bacteria have been exposed to antimicrobial agents. the objective of this study was to test a method using quantitative real-time polymerase chain reaction (qpcr) combined with propidium monoazide (pma), developed for the selective quantification of viable p. gingivalis, a. actinomycetemcomitans, f. nucleatum and total bacteria in an in ... | 2014 | 23581569 |
| peptides: β-cyclodextrin inclusion compounds as highly effective antimicrobial and anti-epithelial proliferation agents. | the use of antimicrobial peptides (amps) as therapeutic agents for periodontal infections has great advantages, such as broad spectrum of action, low toxicity, and limited bacterial resistance. however, their practical use is limited because of the large amount of peptide required to exercise the microbicidal function. | 2013 | 23510146 |