| association between polycystic ovary syndrome, oral microbiota and systemic antibody responses. | polycystic ovary syndrome (pcos) is a hormonal disorder of women that not only is the leading cause of infertility but also shows a reciprocal link with oral health. this study aimed to investigate the hypothesis that the levels of putative periodontal pathogens in saliva and their antibody response in serum are elevated in pcos, compared to systemic health. a total of 125 women were included in four groups; 45 women with pcos and healthy periodontium, 35 women with pcos and gingivitis, 25 syste ... | 2014 | 25232962 |
| the presence of cariogenic and periodontal pathogens in the oral cavity of one-year-old infants delivered pre-term with very low birthweights: a case control study. | recently, the dental literature has focused mainly on the microbial colonization of healthy full-term infants and their mothers or caretakers. however, oral microbial acquisition by premature infants has not been adequately investigated, and the correlation between pre-term birth and the presence of cariogenic and periodontal pathogens has not been determined. the aim of this study was to identify the presence and quantities of representative cariogenic and periodontal pathogens in the oral cavi ... | 2014 | 25178742 |
| massive parallel sequencing provides new perspectives on bacterial brain abscesses. | rapid development within the field of massive parallel sequencing (mps) is about to bring this technology within reach for diagnostic microbiology laboratories. we wanted to explore its potential for improving diagnosis and understanding of polymicrobial infections, using bacterial brain abscesses as an example. we conducted a prospective nationwide study on bacterial brain abscesses. fifty-two surgical samples were included over a 2-year period. the samples were categorized as either spontaneou ... | 2014 | 24671797 |
| subgingival bacteria in ghanaian adolescents with or without progression of attachment loss. | this study describes subgingival bacterial profiles associated with clinical periodontal status in ghanaian adolescents with or without progression of attachment loss. | 2014 | 24834145 |
| a periodontitis-associated multispecies model of an oral biofilm. | while single-species biofilms have been studied extensively, we know notably little regarding multispecies biofilms and their interactions. the purpose of this study was to develop and evaluate an in vitro multispecies dental biofilm model that aimed to mimic the environment of chronic periodontitis. | 2014 | 24778902 |
| characterization of aid1, a novel gene involved in fusobacterium nucleatum interspecies interactions. | the oral opportunistic pathogen fusobacterium nucleatum is known to interact with a large number of different bacterial species residing in the oral cavity. it adheres to a variety of gram-positive bacteria, including oral streptococci via the arginine-inhibitable adhesin radd. in this study, we describe a novel protein encoded by the predicted open reading frame fn1253 that appears to play a role in interspecies interactions of f. nucleatum, particularly with oral streptococci and related gram- ... | 2014 | 24643713 |
| the influence of orthodontic fixed appliances on the oral microbiota: a systematic review. | to investigate whether there is scientific evidence to support the hypothesis that the presence of orthodontic fixed appliances influences the oral microbiota. | 2014 | 24945514 |
| bacteriological analysis of necrotic pulp and fistulae in primary teeth. | primary teeth work as guides for the eruption of permanent dentition, contribute for the development of the jaws, chewing process, preparing food for digestion, and nutrient assimilation. treatment of pulp necrosis in primary teeth is complex due to anatomical and physiological characteristics and high number of bacterial species present in endodontic infections. the bacterial presence alone or in association in necrotic pulp and fistula samples from primary teeth of boys and girls was evaluated ... | 2014 | 24676582 |
| transcriptional responses of treponema denticola to other oral bacterial species. | the classic organization by socransky and coworkers categorized the oral bacteria of the subgingival plaque into different complexes. treponema denticola, porphyromonas gingivalis and tannerella forsythia are grouped into the red complex that is highly correlated with periodontal disease. socransky's work closely associates red with orange complex species such as fusobacterium nucleatum and prevotella intermedia but not with members of the other complexes. while the relationship between species ... | 2014 | 24505483 |
| effects of tongue cleaning on bacterial flora in tongue coating and dental plaque: a crossover study. | the effects of tongue cleaning on reconstruction of bacterial flora in dental plaque and tongue coating itself are obscure. we assessed changes in the amounts of total bacteria as well as fusobacterium nucleatum in tongue coating and dental plaque specimens obtained with and without tongue cleaning. | 2014 | 24423407 |
| total antioxidant capacity and total oxidant status in saliva of periodontitis patients in relation to bacterial load. | the detection of salivary biomarkers has a potential application in early diagnosis and monitoring of periodontal inflammation. however, searching sensitive salivary biomarkers for periodontitis is still ongoing. oxidative stress is supposed to play an important role in periodontitis progression and tissue destruction. in this cross-sectional study, we investigated total antioxidant capacity (tac) and total oxidant status (tos) in saliva of periodontitis patients compared to healthy controls and ... | 2015 | 26779448 |
| polymicrobial oral infection with four periodontal bacteria orchestrates a distinct inflammatory response and atherosclerosis in apoe null mice. | periodontal disease (pd) develops from a synergy of complex subgingival oral microbiome, and is linked to systemic inflammatory atherosclerotic vascular disease (asvd). to investigate how a polybacterial microbiome infection influences atherosclerotic plaque progression, we infected the oral cavity of apoe null mice with a polybacterial consortium of 4 well-characterized periodontal pathogens, porphyromonas gingivalis, treponema denticola, tannerealla forsythia and fusobacterium nucleatum, that ... | 2015 | 26619277 |
| proteomic profiling of host-biofilm interactions in an oral infection model resembling the periodontal pocket. | periodontal infections cause inflammatory destruction of the tooth supporting tissues. we recently developed a dynamic, in vitro periodontal organotypic tissue model in a perfusion bioreactor system, in co-culture with an 11-species subgingival biofilm, which may recapitulate early events during the establishment of periodontal infections. this study aimed to characterize the global proteome regulations in this host-biofilm interaction model. semi-quantitative shotgun proteomics were applied for ... | 2015 | 26525412 |
| humoral immune responses to periodontal pathogens in the elderly. | elderly people are thought to be more susceptible to periodontal disease due to reduced immune function associated with aging. however, little information is available on the nature of immune responses against putative periodontal pathogens in geriatric patients. the purpose of this study was to evaluate the serum igg antibody responses to six periodontal pathogens in geriatric subjects. | 2015 | 26550526 |
| the effect of bamboo leaf extract solution and sodium copper chlorophyllin solution on growth and volatile sulfur compounds production of oral malodor associated some anaerobic periodontal bacteria. | bamboo leaf extract solution (bles) and sodium copper chlorophyllin solution (sccs) are known for their anti-oxidant activities. oral malodor is often related with periodontal pathogens. the present study was undertaken to investigate the anti-bacterial effect of both bles and sccs on anaerobic periodontal bacteria producing oral malodorous volatile sulfur compounds (vsc). | 2015 | 26538799 |
| incorporation of staphylococci into titanium-grown biofilms: an in vitro "submucosal" biofilm model for peri-implantitis. | staphylococcus spp. are postulated to play a role in peri-implantitis. this study aimed to develop a "submucosal" in vitro biofilm model, by integrating two staphylococci into its composition. | 2015 | 26461083 |
| periodontal pathogens invade gingiva and aortic adventitia and elicit inflammasome activation in αvβ6 integrin-deficient mice. | the american heart association supports an association between periodontal diseases and atherosclerosis but not a causal association. this study explores the use of the integrin β6(-/-) mouse model to study the causality. we investigated the ability of a polymicrobial consortium of porphyromonas gingivalis, treponema denticola, tannerella forsythia, and fusobacterium nucleatum to colonize the periodontium and induce local and systemic inflammatory responses. polymicrobially infected itgβ6(-/-) m ... | 2015 | 26371120 |
| periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased dominican adults. | the objective of this study was to compare the periodontopathogen prevalence and tetracycline resistance genes in dominican patients with different periodontal conditions. | 2015 | 26121972 |
| fusobacterium nucleatum alters atherosclerosis risk factors and enhances inflammatory markers with an atheroprotective immune response in apoe(null) mice. | the american heart association supports an association between periodontal disease (pd) and atherosclerotic vascular disease (asvd) but does not as of yet support a causal relationship. recently, we have shown that major periodontal pathogens porphyromonas gingivalis and treponema denticola are causally associated with acceleration of aortic atherosclerosis in apoenull hyperlipidemic mice. the aim of this study was to determine if oral infection with another significant periodontal pathogen fuso ... | 2015 | 26079509 |
| effects of hangeshashinto on growth of oral microorganisms. | oral mucositis (om) in cancer patients induced by chemotherapy or radiotherapy has a significant impact on quality of life, and causes considerable morbidity. oral microorganisms are likely to intensify the inflammatory process and aggravate the formation of ulcers. hangeshashinto (hst), a japanese kampo medicine, has been reported to be effective when used as a gargle for the treatment of om. to clarify the effects of hst on oral microorganisms, we assessed its antimicrobial activity against 27 ... | 2015 | 26170876 |
| coaggregation and biofilm growth of granulicatella spp. with fusobacterium nucleatum and aggregatibacter actinomycetemcomitans. | members of fastidious granulicatella and aggregatibacter genera belong to normal oral flora bacteria that can cause serious infections, such as infective endocarditis. aggregatibacter actinomycetemcomitans has long been implicated in aggressive periodontitis, whereas dna-based methods only recently showed an association between granulicatella spp. and dental diseases. as bacterial coaggregation is a key phenomenon in the development of oral and nonoral multispecies bacterial communities it would ... | 2015 | 26025449 |
| development of in vitro denture biofilm models for halitosis related bacteria and their application in testing the efficacy of antimicrobial agents. | since dentures can serve as a reservoir for halitosis-causing oral bacteria, halitosis development is a concern for denture wearers. in this study, we surveyed the prevalence of four selected halitosis-related species (fusobacterium nucleatum, tannerella forsythia, veillonella atypica and klebsiella pneumoniae) in clinical denture plaque samples, and developed denture biofilm models for these species in vitro to facilitate assessment of antimicrobial treatment efficacy. design : denture plaque f ... | 2015 | 25926895 |
| prelude to oral microbes and chronic diseases: past, present and future. | associations between oral and systemic health are ancient. oral opportunistic bacteria, particularly, porphyromonas gingivalis and fusobacterium nucleatum, have recently been deviated from their traditional roles as periodontal pathogens and arguably ascended to central players based on their participations in complex co-dependent mechanisms of diverse systemic chronic diseases risk and pathogenesis, including cancers, rheumatoid-arthritis, and diabetes. | 2015 | 25813714 |
| laser supported reduction of specific microorganisms in the periodontal pocket with the aid of an er,cr:ysgg laser: a pilot study. | the aim of this study was to evaluate the effectiveness of a radial firing tip of an er,cr:ysgg laser as an adjunct to a nonsurgical periodontal treatment. | 2015 | 25879057 |
| bacterial profile of aggressive periodontitis in morocco: a cross-sectional study. | aggressive periodontitis (agp) is one of the most severe forms of periodontal diseases. in morocco, aggregatibacter actinomycetemcomitans has been strongly associated with agp, however limited knowledge is available about the implication of other periodontal pathogens in this entity. therefore, the main aim of this study was to evaluate the composition of the subgingival microbiota in moroccan patients with agp. | 2015 | 25888404 |
| the biofilm community-rebels with a cause. | oral biofilms are one of the most complex and diverse ecosystem developed by successive colonization of more than 600 bacterial taxa. development starts with the attachment of early colonizers such as actinomyces species and oral streptococci on the acquired pellicle and tooth enamel. these bacteria not only adhere to tooth surface but also interact with each other and lay foundation for attachment of bridging colonizer such as fusobacterium nucleatum followed by late colonizers including the re ... | 2015 | 26120510 |
| photoinactivation using visible light plus water-filtered infrared-a (vis+wira) and chlorine e6 (ce6) eradicates planktonic periodontal pathogens and subgingival biofilms. | alternative treatment methods for pathogens and microbial biofilms are required due to the widespread rise in antibiotic resistance. antimicrobial photodynamic therapy (apdt) has recently gained attention as a novel method to eradicate pathogens. the aim of this study was to evaluate the antimicrobial effects of a novel apdt method using visible light (vis) and water infiltrated infrared a (wira) in combination with chlorine e6 (ce6) against different periodontal pathogens in planktonic form and ... | 2016 | 27965635 |
| the danger signal extracellular atp is an inducer of fusobacterium nucleatum biofilm dispersal. | plaque biofilm is the primary etiological agent of periodontal disease. biofilm formation progresses through multiple developmental stages beginning with bacterial attachment to a surface, followed by development of microcolonies and finally detachment and dispersal from a mature biofilm as free planktonic bacteria. tissue damage arising from inflammatory response to biofilm is one of the hallmark features of periodontal disease. a consequence of tissue damage is the release of atp from within t ... | 2016 | 27909688 |
| salivary periodontopathic bacteria in children and adolescents with down syndrome. | to assess and compare salivary periodontopathic bacteria between groups of down syndrome and non-down syndrome children and adolescents. | 2016 | 27727287 |
| emerging role of bacteria in oral carcinogenesis: a review with special reference to perio-pathogenic bacteria. | oral cancer, primarily oral squamous cell carcinoma (oscc), continues to be a major global health problem with high incidence and low survival rates. while the major risk factors for this malignancy, mostly lifestyle related, have been identified, around 15% of oral cancer cases remain unexplained. in light of evidence implicating bacteria in the aetiology of some cancer types, several epidemiological studies have been conducted in the last decade, employing methodologies ranging from traditiona ... | 2016 | 27677454 |
| distinguishing the signals of gingivitis and periodontitis in supragingival plaque: a cross-sectional cohort study in malawi. | periodontal disease ranges from gingival inflammation (gingivitis) to the inflammation and loss of tooth-supporting tissues (periodontitis). previous research has focused mainly on subgingival plaque, but supragingival plaque composition is also known to be associated with disease. quantitative modeling of bacterial abundances across the natural range of periodontal severities can distinguish which features of disease are associated with particular changes in composition. we assessed a cross-sec ... | 2016 | 27520811 |
| impact of aging on trem-1 responses in the periodontium: a cross-sectional study in an elderly population. | aging is associated with altered immune response, which increases susceptibility to infections. strem-1 is involved in the amplification of the inflammatory response to bacterial infection. the present cross-sectional study aims to investigate local strem-1 levels in gingival crevicular fluid (gcf) as well as key periodontal pathogen levels in the subgingival plaque in an elderly cohort with periodontal health, gingivitis, and chronic periodontitis (cp). | 2016 | 27542376 |
| putative periodontopathic bacteria and herpesviruses in pregnant women: a case-control study. | little is known about herpesvirus and putative periodontopathic bacteria in maternal chronic periodontitis. the present case-control study aimed to explore the potential relationship between putative periodontopathic bacteria and herpesviruses in maternal chronic periodontitis.saliva samples were collected from 36 pregnant women with chronic periodontitis (cases) and 36 pregnant women with healthy periodontal status (controls). six putative periodontopathic bacteria (porphyromonas gingivalis [pg ... | 2016 | 27301874 |
| adjunctive use of essential oils following scaling and root planing -a randomized clinical trial. | hitherto no study has been published on the effect of the adjunctive administration of essential oils following scaling and root planing (srp). this study describes the effect of a mouthrinse consisting of essential oils (cymbopogon flexuosus, thymus zygis and rosmarinus officinalis) following srp by clinical and microbiological variables in patients with generalized moderate chronic periodontitis. | 2016 | 27266517 |
| quantitative molecular detection of 19 major pathogens in the interdental biofilm of periodontally healthy young adults. | in oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. the interdental spaces are the source of many hypotheses regarding their potential associations with and/or causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative disease, and depression. this pcr study is the first to describe the interdental microbiota in hea ... | 2016 | 27313576 |
| accuracy of commercial kits and published primer pairs for the detection of periodontopathogens. | despite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. the aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens. | 2016 | 27020914 |
| microbiological efficacy of photodynamic therapy as an adjunct to non-surgical periodontal treatment: a clinical trial. | the efficiency of routine scaling and root planning is negatively influenced by the tooth anatomy and residual bacteria all possibly affecting the treatment outcomes in future. the present study compared the microbiologic effectiveness of the photodynamic therapy (pdt) as an adjunctive treatment modality for nonsurgical treatment in chronic periodontitis. | 2016 | 27330709 |
| oral microbiota species in acute apical endodontic abscesses. | acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. the objective of this study was to identify and compare the oral microbiota in patients (n=18) exhibiting acute apical abscesses, originating from the demographic region in portland, oregon. the study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. | 2016 | 26983837 |
| extensive identification of bacterial riboflavin transporters and their distribution across bacterial species. | riboflavin, the precursor for the cofactors flavin mononucleotide (fmn) and flavin adenine dinucleotide, is an essential metabolite in all organisms. while the functions for de novo riboflavin biosynthesis and riboflavin import may coexist in bacteria, the extent of this co-occurrence is undetermined. the ribm, ribn, rfuabcd and the energy-coupling factor-ribu bacterial riboflavin transporters have been experimentally characterized. in addition, impx, rfnt and ribxy are proposed as riboflavin tr ... | 2015 | 25938806 |
| porphyromonas gingivalis entry into gingival epithelial cells modulated by fusobacterium nucleatum is dependent on lipid rafts. | host cell invasion by a major periodontal pathogen, porphyromonas gingivalis, has been proposed as an important mechanism involved in host-pathogen interactions in periodontal and cardiovascular diseases. the present study sought to gain insight into the underlying mechanism(s) involved in previously demonstrated fusobacterial modulation of host cell invasion by p. gingivalis. an immortalized human gingival cell line ca9-22 was dually infected with p. gingivalis atcc 33277 and fusobacterium nucl ... | 2012 | 23034475 |
| co-occurrence of anaerobic bacteria in colorectal carcinomas. | numerous cancers have been linked to microorganisms. given that colorectal cancer is a leading cause of cancer deaths and the colon is continuously exposed to a high diversity of microbes, the relationship between gut mucosal microbiome and colorectal cancer needs to be explored. metagenomic studies have shown an association between fusobacterium species and colorectal carcinoma. here, we have extended these studies with deeper sequencing of a much larger number (n = 130) of colorectal carcinoma ... | 2013 | 24450771 |
| high-level antimicrobial efficacy of representative mediterranean natural plant extracts against oral microorganisms. | nature is an unexplored reservoir of novel phytopharmaceuticals. since biofilm-related oral diseases often correlate with antibiotic resistance, plant-derived antimicrobial agents could enhance existing treatment options. therefore, the rationale of the present report was to examine the antimicrobial impact of mediterranean natural extracts on oral microorganisms. five different extracts from olea europaea, mastic gum, and inula viscosa were tested against ten bacteria and one candida albicans s ... | 2014 | 25054150 |
| isph-rps1 and isph-ubia: "rosetta stone" proteins. | the protein isph, (e)-1-hydroxy-2-methyl-but-2-enyl 4-diphosphate (hmppp) reductase, is an essential 4fe-4s cluster-containing protein in the methylerythritol phosphate pathway for isoprenoid biosynthesis. using a sequence similarity network we found that there are >400 isph proteins that are about twice as large as most of the isphs studied to date since their isph domains are fused to either the ribosomal protein s1 (rps1), or to a ubia (4-hydroxybenzoate octaprenyltransferase)-like protein. m ... | 2015 | 26865948 |
| antibacterial activity of myristica fragrans against oral pathogens. | myristica fragrans houtt is mostly cultivated for spices in penang island, malaysia. the ethyl acetate and ethanol extracts of flesh, mace and seed of myristica fragrans was evaluated the bactericidal potential against three gram-positive cariogenic bacteria (streptococcus mutans atcc 25175, streptococcus mitis atcc 6249, and streptococcus salivarius atcc 13419) and three gram-negative periodontopathic bacteria (aggregatibacter actinomycetemcomitans atcc 29522, porphyromonas gingivalis atcc 3327 ... | 2012 | 23049613 |
| association of plasma 25-hydroxyvitamin d concentrations and pathogenic oral bacteria in postmenopausal females. | previous findings of an association between 25-hydroxyvitamin d [25(oh)d] concentrations and periodontal disease may be partially explained by the antimicrobial properties of vitamin d. to the best of the authors' knowledge, no study has investigated the association between 25(oh)d and pathogenic oral bacteria, a putative cause of periodontal disease. | 2013 | 24261910 |
| effect of an oxygenating agent on oral bacteria in vitro and on dental plaque composition in healthy young adults. | oral bacteria live in symbiosis with the host. therefore, when mouthwashes are indicated, selective inhibition of taxa contributing to disease is preferred instead of broad-spectrum antimicrobials. the potential selectivity of an oxygenating mouthwash, ardox-x® (ax), has not been assessed. the aim of this study was to determine the antimicrobial potential of ax and the effects of a twice-daily oral rinse on dental plaque composition. | 2014 | 25101249 |
| synergic phototoxic effect of visible light or gallium-arsenide laser in the presence of different photo-sensitizers on porphyromonas gingivalis and fusobacterium nucleatum. | according to the development of resistant strains of pathogenic bacteria following treatment with antimicrobial chemotherapeutic agents, alternative approaches such as lethal photosensitization are being used. the aim of this study was to evaluate the effect of visible light and laser beam radiation in conjugation with three different photosensitizers on the survival of two main periodontopathogenic bacteria including porphyromonas gingivalis and fusobacterium nucleatum in different exposure per ... | 2015 | 26288621 |
| abnormal pregnancy outcomes in mice using an induced periodontitis model and the haematogenous migration of fusobacterium nucleatum sub-species to the murine placenta. | to investigate if there is subspecies specific migration to the placenta by fusobacterium nucleatum (fn) and to determine whether experimentally induced periodontitis results in adverse pregnancy outcomes (apo) in mice. | 2015 | 25806806 |
| comparison of the detection of periodontal pathogens in bacteraemia after tooth brushing by culture and molecular techniques. | the prevalence and amounts of periodontal pathogens detected in bacteraemia samples after tooth brushing-induced by means of four diagnostic technique, three based on culture and one in a molecular-based technique, have been compared in this study. | 2016 | 26946197 |
| in vitro effect of porphyromonas gingivalis methionine gamma lyase on biofilm composition and oral inflammatory response. | methanethiol (methyl mercaptan) is an important contributor to oral malodour and periodontal tissue destruction. porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum are key oral microbial species that produce methanethiol via methionine gamma lyase (mgl) activity. the aim of this study was to compare an mgl knockout strain of p. gingivalis with its wild type using a 10-species biofilm co-culture model with oral keratinocytes and its effect on biofilm composition and infla ... | 2016 | 28033374 |
| porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of prommp9 and its activation. | recent epidemiological studies have revealed a significant association between periodontitis and oral squamous cell carcinoma (oscc). furthermore, matrix metalloproteinase 9 (mmp9) is implicated in the invasion and metastasis of tumour cells. we examined the involvement of porphyromonas gingivalis, a periodontal pathogen, in oscc invasion through induced expression of prommp and its activation. prommp9 was continuously secreted from carcinoma sas cells, while p. gingivalis infection increased pr ... | 2013 | 23991831 |
| fusobacterium nucleatum-induced impairment of autophagic flux enhances the expression of proinflammatory cytokines via ros in caco-2 cells. | fusobacterium nucleatum (f. nucleatum) plays a critical role in gastrointestinal inflammation. however, the exact mechanism by which f. nucleatum contributes to inflammation is unclear. in the present study, it was revealed that f. nucleatum could induce the production of proinflammatory cytokines (il-8, il-1β and tnf-α) and reactive oxygen species (ros) in caco-2 colorectal) adenocarcinoma cells. furthermore, ros scavengers (nac or tiron) could decrease the production of proinflammatory cytokin ... | 2016 | 27828984 |
| target identification in fusobacterium nucleatum by subtractive genomics approach and enrichment analysis of host-pathogen protein-protein interactions. | fusobacterium nucleatum, a well studied bacterium in periodontal diseases, appendicitis, gingivitis, osteomyelitis and pregnancy complications has recently gained attention due to its association with colorectal cancer (crc) progression. treatment with berberine was shown to reverse f. nucleatum-induced crc progression in mice by balancing the growth of opportunistic pathogens in tumor microenvironment. intestinal microbiota imbalance and the infections caused by f. nucleatum might be regulated ... | 2016 | 27176600 |
| diverse allosteric and catalytic functions of tetrameric d-lactate dehydrogenases from three gram-negative bacteria. | nad-dependent d-lactate dehydrogenases (d-ldhs) reduce pyruvate into d-lactate with oxidation of nadh into nad(+). although non-allosteric d-ldhs from lactobacilli have been extensively studied, the catalytic properties of allosteric d-ldhs from gram-negative bacteria except for escherichia coli remain unknown. we characterized the catalytic properties of d-ldhs from three gram-negative bacteria, fusobacterium nucleatum (fnldh), pseudomonas aeruginosa (paldh), and e. coli (ecldh) to gain an insi ... | 2014 | 25401076 |
| morphological and physiological changes induced by contact-dependent interaction between candida albicans and fusobacterium nucleatum. | candida albicans and fusobacterium nucleatum are well-studied oral commensal microbes with pathogenic potential that are involved in various oral polymicrobial infectious diseases. recently, we demonstrated that f. nucleatum atcc 23726 coaggregates with c. albicans sn152, a process mainly mediated by fusobacterial membrane protein radd and candida cell wall protein flo9. the aim of this study was to investigate the potential biological impact of this inter-kingdom interaction. we found that f. n ... | 2016 | 27295972 |
| a new type of na(+)-driven atp synthase membrane rotor with a two-carboxylate ion-coupling motif. | the anaerobic bacterium fusobacterium nucleatum uses glutamate decarboxylation to generate a transmembrane gradient of na⁺. here, we demonstrate that this ion-motive force is directly coupled to atp synthesis, via an f₁f₀-atp synthase with a novel na⁺ recognition motif, shared by other human pathogens. molecular modeling and free-energy simulations of the rotary element of the enzyme, the c-ring, indicate na⁺ specificity in physiological settings. consistently, activity measurements showed na⁺ s ... | 2013 | 23824040 |
| robust species taxonomy assignment algorithm for 16s rrna ngs reads: application to oral carcinoma samples. | usefulness of next-generation sequencing (ngs) in assessing bacteria associated with oral squamous cell carcinoma (oscc) has been undermined by inability to classify reads to the species level. | 2015 | 26426306 |
| toll-like receptor 2-dependent activity of native major outer membrane protein proteosomes of chlamydia trachomatis. | chlamydia trachomatis is the most common sexually transmitted bacterial pathogen and the etiologic agent of blinding trachoma. intracellular signaling pathways leading to host cell inflammation and innate immunity to chlamydia include those mediated by toll-like receptors (tlrs) and nucleotide binding oligomerization domain 1 (nod1) protein. in epithelial cells, tlr-dependent signaling contributes to local immune responses via induction of inflammatory mediators. there is evidence that tlr3, tlr ... | 2013 | 23132491 |
| arginine-ornithine antiporter arcd controls arginine metabolism and interspecies biofilm development of streptococcus gordonii. | arginine is utilized by the oral inhabitant streptococcus gordonii as a substrate of the arginine deiminase system (ads), eventually producing atp and nh3, the latter of which is responsible for microbial resistance to ph stress. s. gordonii expresses a putative arginine-ornithine antiporter (arcd) whose function has not been investigated despite relevance to the ads and potential influence on inter-bacterial communication with periodontal pathogens that utilize amino acids as a main energy sour ... | 2015 | 26085091 |
| fap2 of fusobacterium nucleatum is a galactose-inhibitable adhesin involved in coaggregation, cell adhesion, and preterm birth. | fusobacterium nucleatum is a common oral anaerobe involved in periodontitis that is known to translocate and cause intrauterine infections. in the oral environment, f. nucleatum adheres to a large diversity of species, facilitating their colonization and creating biological bridges that stabilize the multispecies dental biofilm. many of these interactions (called coadherences or coaggregations) are galactose sensitive. galactose-sensitive interactions are also involved in the binding of f. nucle ... | 2015 | 25561710 |
| fusobacterium nucleatum: a rare cause of pyogenic liver abscess. | while pyogenic liver abscesses are uncommon, they are associated with significant mortality and morbidity. most pyogenic liver abscesses are polymicrobial and are caused by enteric bacteria and anaerobes. rarely, mono-microbial infections may occur, typically in immunocompromised individuals. we report the unusual case of a 69 year-old immunocompetent female who developed a pyogenic liver abscess due to fusobacterium nucleatum infection, likely from a dental source. poor oropharyngeal hygiene se ... | 2015 | 26101735 |
| high individuality of respiratory bacterial communities in a large cohort of adult cystic fibrosis patients under continuous antibiotic treatment. | routine clinical diagnostics of cf patients focus only on a restricted set of well-known pathogenic species. recent molecular studies suggest that infections could be polymicrobial with many bacteria not detected by culture-based diagnostics. | 2015 | 25671713 |
| adjunctive azithromycin in the treatment of aggressive periodontitis: microbiological findings of a 12-month randomized clinical trial. | to compare the subgingival microbiological outcomes of azithromycin or placebo as adjuncts to scaling and root planing (srp) in the treatment of aggressive periodontitis (agp), and to secondarily evaluate the microbiological effect of supragingival scaling in agp patients. | 2012 | 22445846 |
| analysis of the expression of nlrp3 and aim2 in periapical lesions with apical periodontitis and microbial analysis outside the apical segment of teeth. | to detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (nlrp3) and the absent in melanoma 2 (aim2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth. | 2017 | 28193569 |
| oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients. | the objective of this study was to identify the dna of oral bacteria in placental samples from women with and without periodontitis who had or had not had preterm births and/or low birthweight (pb/lbw) neonates. | 2015 | 26259070 |
| coinfection of fusobacterium nucleatum and actinomyces israelii in mastoiditis diagnosed by next-generation dna sequencing. | some bacterial infections involve potentially complex mixtures of species that can now be distinguished using next-generation dna sequencing. we present a case of mastoiditis where gram stain, culture, and molecular diagnosis were nondiagnostic or discrepant. next-generation sequencing implicated coinfection of fusobacterium nucleatum and actinomyces israelii, resolving these diagnostic discrepancies. | 2014 | 24574281 |
| analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16s rdna gene sequence. | to analyze and characterize the predominant bacterial flora associated with peri-implantitis by using culture techniques under obligate anaerobic conditions and 16s rdna gene sequences. | 2013 | 24278920 |
| the predominant bacteria isolated from radicular cysts. | to detect predominant bacteria associated with radicular cysts and discuss in light of the literature. | 2013 | 24011184 |
| microbiological diversity of peri-implantitis biofilm by sanger sequencing. | to examine the microbial diversity associated with implants with or without peri-implantitis and to identify differences between the bacterial communities in these clinical conditions. | 2014 | 23845046 |
| osteomyelitis of a long bone due to fusobacterium nucleatum and actinomyces meyeri in an immunocompetent adult: a case report and literature review. | fusobacterium species are uncommon causes of osteomyelitis. these organisms are normal flora of the oral cavity. therefore, they mostly cause osteomyelitis of the head and neck. hematogenous osteomyelitis at distant sites other than the head and neck has rarely been reported in pediatric or immunocompromised patients. here, we report the first case of osteomyelitis of a long bone combined with a muscle abscess due to fusobacterium nucleatum in an otherwise healthy adult. | 2012 | 22817336 |
| novel bioactive root canal sealer to inhibit endodontic multispecies biofilms with remineralizing calcium phosphate ions. | the objectives of this study were to: (1) develop a bioactive endodontic sealer via dimethylaminohexadecyl methacrylate (dmahdm), 2-methacryloyloxyethyl phosphorylcholine (mpc) and nanoparticles of amorphous calcium phosphate (nacp) for the first time; and (2) evaluate inhibition of early-stage and mature multispecies endodontic biofilm, bond strength to root canal dentine, and calcium (ca) and phosphate (p) ion release. | 2017 | 28223198 |
| three-species biofilm model onto plasma-treated titanium implant surface. | in this study, titanium (ti) was modified with biofunctional and novel surface by micro-arc oxidation (mao) and glow discharge plasma (gdp) and we tested the development of a three-species periodontopatogenic biofilm onto the treated commercially-pure titanium (cpti) surfaces. machined and sandblasted surfaces were used as control group. several techniques for surface characterizations and monoculture on bone tissue cells were performed. a multispecies biofilm composed of streptococcus sanguinis ... | 2017 | 28131960 |
| the use of bacteriophages to biocontrol oral biofilms. | infections induced by oral biofilms include caries, as well as periodontal, and peri-implant disease, and may influence quality of life, systemic health, and expenditure. as bacterial biofilms are highly resistant and resilient to conventional antibacterial therapy, it has been difficult to combat these infections. an innovative alternative to the biocontrol of oral biofilms could be to use bacteriophages or phages, the viruses of bacteria, which are specific, non-toxic, self-proliferating, and ... | 2017 | 28108235 |
| development of a cavity disinfectant containing antibacterial monomer mdpb. | an experimental cavity disinfectant (acc) that is intended to be used for various direct and indirect restorations was prepared by adding an antibacterial monomer 12-methacryloyloxydodecylpyridinum bromide (mdpb) at 5% into 80% ethanol. the antibacterial effectiveness of acc and its influences on the bonding abilities of resin cements were investigated. to examine the antibacterial activity of unpolymerized mdpb, the minimum inhibitory and bactericidal concentrations (mic and mbc) were determine ... | 2016 | 27558263 |
| bifidobacteria inhibit the growth of porphyromonas gingivalis but not of streptococcus mutans in an in vitro biofilm model. | there is growing interest in the use of probiotic bifidobacteria for enhancement of the therapy, and in the prevention, of oral microbial diseases. however, the results of clinical studies assessing the effects of bifidobacteria on the oral microbiota are controversial, and the mechanisms of actions of probiotics in the oral cavity remain largely unknown. in addition, very little is known about the role of commensal bifidobacteria in oral health. our aim was to study the integration of the probi ... | 2016 | 27061393 |
| influence of different instrumentation modalities on the surface characteristics and biofilm formation on dental implant neck, in vitro. | to evaluate surface characteristics of implants after using different instruments and biofilm formation following instrumentation. | 2017 | 27000771 |
| effect of roselle calyx extract on in vitro viability and biofilm formation ability of oral pathogenic bacteria. | to investigate the effect of the roselle calyx extract (rce) (hibiscus sabdariffa l.) on the in vitro viability and biofilm formation ability of oral pathogenic bacteria. | 2016 | 26919939 |
| antimicrobial activity of protamine against oral microorganisms. | protamine is an arginine-rich polycationic protein extracted from sperm cells of vertebrates including fishes such as salmon. the purpose of this study was to investigate the suppressive effects of protamine on the growth of oral pathogens for possible usage in dental materials. minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) were determined by the microdilution method. twelve strains of oral viridans streptococci, actinomyces naeslundii, actinomyces odontolyt ... | 2015 | 26699859 |
| antibacterial activity of commercially available plant-derived essential oils against oral pathogenic bacteria. | this work investigated the antibacterial activity of 15 commercially available plant-derived essential oils (eos) against a panel of oral pathogens. the broth microdilution method afforded the minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs) of the assayed eos. the eo obtained from cinnamomum zeylanicum (lauraceae) (cz-eo) displayed moderate activity against fusobacterium nucleatum (mic and mbc = 125 μg/ml), actinomyces naeslundii (mic and mbc = 125 μg/ml), ... | 2016 | 26165725 |
| [antibacterial activity of synthetic antimicrobial decapeptide against oral bacteria]. | this study aims to evaluate the antimicrobial activity of decapeptide, a novel antimicrobial peptide, against several major cariogenic and periodontopathogenic bacteria in vitro. methods in this study, we investigated the antimicrobial activity of decapeptide against streptococcus mutans (s. mutans), streptococcus sobrinus, lactobacillus acidophilus, streptococcus sanguis, streptococcus gordonii, actinomyces viscosus, actinomyces naeslundii, porphyromonas gingivalis, prevotella intermedia, fusob ... | 2014 | 25665430 |
| individual growth detection of bacterial species in an in vitro oral polymicrobial biofilm model. | most in vitro studies on the antibacterial effects of antiseptics have used planktonic bacteria in monocultures. however, this study design does not reflect the in vivo situation in oral cavities harboring different bacterial species that live in symbiotic relationships in biofilms. the aim of this study was to establish a simple in vitro polymicrobial model consisting of only three bacterial strains of different phases of oral biofilm formation to simulate in vivo oral conditions. therefore, we ... | 2014 | 25119373 |
| an in vitro biofilm model associated to dental implants: structural and quantitative analysis of in vitro biofilm formation on different dental implant surfaces. | the impact of implant surfaces in dental biofilm development is presently unknown. the aim of this investigation was to assess in vitro the development of a complex biofilm model on titanium and zirconium implant surfaces, and to compare it with the same biofilm formed on hydroxyapatite surface. | 2014 | 25110288 |
| microbial evaluation of traumatized teeth treated with triple antibiotic paste or calcium hydroxide with 2% chlorhexidine gel in pulp revascularization. | revascularization outcome depends on microbial elimination because apical repair will not happen in the presence of infected tissues. this study evaluated the microbial composition of traumatized immature teeth and assessed their reduction during different stages of the revascularization procedures performed with 2 intracanal medicaments. | 2014 | 24862703 |
| in vitro evaluation of a multispecies oral biofilm on different implant surfaces. | biofilm accumulation on implant surfaces is one of the most important factors for early and late implant failure. because of the related clinical implications, the aim of this in vitro study was to compare the bacterial cell attachment of a four-species oral biofilm on titanium discs of purity grade 2 and 4, with machined surfaces and etched-thermochemically modified with avantblast®. the in vitro biofilm model was composed of early (actinomyces naeslundii, streptococcus gordonii), secondary (ve ... | 2014 | 24770899 |
| salivary pellicle composition and multispecies biofilm developed on titanium nitrided by cold plasma. | the aim of this study was to evaluate the composition of the salivary pellicle (sp) and multispecies biofilm developed on titanium nitrided by cold plasma. | 2014 | 24769315 |
| multiplex fluorescence in situ hybridization (m-fish) and confocal laser scanning microscopy (clsm) to analyze multispecies oral biofilms. | multiplex fluorescence in situ hybridization (m-fish) constitutes a favorable microbiological method for the analysis of spatial distribution of highly variable phenotypes found in multispecies oral biofilms. the combined use of confocal laser scanning microscopy (clsm) produces high-resolution three-dimensional (3d) images of individual bacteria in their natural environment. here, we describe the application of m-fish on early (streptococcus spp., actinomyces naeslundii) and late colonizers (fu ... | 2014 | 24664826 |
| dental caries and microbiota in children with black stain and non-discoloured dental plaque. | we aimed to assess caries experience and microbiota in systemically healthy children with black stain (bs) and non-discoloured plaque. | 2014 | 24335143 |
| characterization and in vitro properties of oral lactobacilli in breastfed infants. | lactobacillus species can contribute positively to general and oral health and are frequently acquired by breastfeeding in infancy. the present study aimed to identify oral lactobacilli in breast and formula-fed 4 month-old infants and to evaluate potential probiotic properties of the dominant lactobacillus species detected. saliva and oral swab samples were collected from 133 infants who were enrolled in a longitudinal study (n=240) examining the effect of a new infant formula on child growth a ... | 2013 | 23945215 |
| photodynamic biofilm inactivation by sapyr--an exclusive singlet oxygen photosensitizer. | prevention and control of biofilm-growing microorganisms are serious problems in public health due to increasing resistances of some pathogens against antimicrobial drugs and the potential of these microorganisms to cause severe infections in patients. therefore, alternative approaches that are capable of killing pathogens are needed to supplement standard treatment modalities. one alternative is the photodynamic inactivation of bacteria (pib). the lethal effect of pib is based on the principle ... | 2013 | 23891675 |
| characterization and application of a flow system for in vitro multispecies oral biofilm formation. | bacteria in the oral cavity grow in the form of biofilms; these structures are subject to constant saliva or gingival crevicular fluid flow conditions. the aims of this study were: (i) to develop and to characterize an in-vitro biofilm model with oral bacteria growing under flow and shear conditions; and (ii) to demonstrate the usefulness of the model for evaluating the activity of three antiplaque agents. | 2014 | 23815431 |
| effects of short-chain fatty acids on actinomyces naeslundii biofilm formation. | actinomyces naeslundii is an early colonizer and has important roles in the development of the oral biofilm. short-chain fatty acids (scfa) are secreted extracellularly as a product of metabolism by gram-negative anaerobes, e.g. porphyromonas gingivalis and fusobacterium nucleatum; and the scfa may affect biofilm development with interaction between a. naeslundii and gram-negative bacteria. our aim was to investigate the effects of scfa on biofilm formation by a. naeslundii and to determine the ... | 2013 | 23731652 |
| in vivo study of the initial bacterial adhesion on different implant materials. | biofilm formation on implant materials plays a major role in the aetiology of periimplantitis. the aim of this study was to examine in vivo the initial bacterial adhesion on six different implant materials. | 2013 | 23694907 |
| effect of metalloporphyrins on red autofluorescence from oral bacteria. | the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ... | 2013 | 23659237 |
| antimicrobial activity of ethanol extracts of laminaria japonica against oral microorganisms. | laminaria japonica is a brown alga, which is consumed widely in korea, japan, and china. this study investigated the antimicrobial activity of ethanol extracts of l. japonica against oral microbial species to assess the possible application of l. japonica extracts in dental care products. the minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) were determined in culture medium using a microdilution method. the mics of ethanol extracts of l. japonica with oral stre ... | 2013 | 23583539 |
| oral epithelial cell responses to multispecies microbial biofilms. | this report describes the use of a novel model of multispecies biofilms to stimulate profiles of cytokines/chemokines from oral epithelial cells that contribute to local inflammation in the periodontium. streptococcus gordonii (sg)/s. oralis (so)/s. sanguinis (ss) and sg/fusobacterium nucleatum (fn)/porphyromonas gingivalis (pg) biofilms elicited significantly elevated levels of il-1α and showed synergistic stimulatory activity compared with an additive effect of the 3 individual bacteria. only ... | 2013 | 23300185 |
| in vitro evaluation of mtad and nisin in combination against common pathogens associated with root canal infection. | many pathogenic microorganisms were found in an infected root canal. the object of this study was to evaluate the effect of mtad in combination with nisin on the pathogens associated with root canal infection. | 2012 | 22414835 |
| subgingival microbiota in adult down syndrome periodontitis. | the subgingival microbiota in down syndrome and non-down syndrome adults receiving periodic dental care was examined for 40 bacterial species using checkerboard dna-dna hybridization and the results were related to clinical periodontal attachment loss. | 2012 | 22221039 |
| development of a novel multi-triplex qpcr method for the assessment of bacterial community structure in oral populations. | gingivitis and dental caries are two of the most predominant diseases in humans. both conditions are easily treated with the removal of the plaque biofilm by brushing or the use of oral hygiene products. in both cases, pathogenic taxa found within the plaque biofilm are the causal agents of the disease. actinomyces naeslundii, fusobacterium nucleatum and prevotalla intermedia have all been implicated in the development of gingivitis, while streptococcus mutans is the main organism associated wit ... | 2010 | 23766283 |
| multi-centre evaluation of mass spectrometric identification of anaerobic bacteria using the vitek® ms system. | accurate and timely identification of anaerobic bacteria is critical to successful treatment. classic phenotypic methods for identification require long turnaround times and can exhibit poor species level identification. matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) is an identification method that can provide rapid identification of anaerobes. we present a multi-centre study assessing the clinical performance of the vitek(®) ms in the identification ... | 2014 | 23927597 |
| microbiological evaluation of bacterial and mycotic seal in implant systems with different implant-abutment interfaces and closing torque values. | the aim of this study was to evaluate the possible leakage of 3 species of bacteria (streptococcus sanguinis, fusobacterium nucleatum, and actinomyces odontolyticus) and of candida albicans and candida glabrata in osseointegrated implants with different implant-abutment interface (iai) geometry. | 2013 | 23782848 |