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quantitative analysis of periodontal pathogens present in the saliva of geriatric subjects.at present, information regarding periodontal disease in geriatric patients is scarce. the purpose of this study was to quantify the periodontal pathogens present in the saliva of korean geriatric patients and assess the relationship between the bacterial levels and the periodontal condition.201324040571
bacterial community development in experimental gingivitis.current knowledge of the microbial composition of dental plaque in early gingivitis is based largely on microscopy and cultural methods, which do not provide a comprehensive description of oral microbial communities. this study used 454-pyrosequencing of the v1-v3 region of 16s rrna genes (approximately 500 bp), and bacterial culture, to characterize the composition of plaque during the transition from periodontal health to gingivitis. a total of 20 healthy volunteers abstained from oral hygiene ...201323967169
mobile microbiome: oral bacteria in extra-oral infections and inflammation.the link between oral infections and adverse systemic conditions has attracted much attention in the research community. several mechanisms have been proposed, including spread of the oral infection due to transient bacteremia resulting in bacterial colonization in extra-oral sites, systemic injury by free toxins of oral pathogens, and systemic inflammation caused by soluble antigens of oral pathogens. mounting evidence supports a major role of the systemic spread of oral commensals and pathogen ...201323625375
short-term microbiological effects of scaling and root planing and essential-oils mouthwash in chinese adults.to assess the short-term effect of scaling and root planing (srp) and essential-oils mouthwash on the levels of specific bacteria in chinese adults.201323645178
the surface layer of tannerella forsythia contributes to serum resistance and oral bacterial coaggregation.tannerella forsythia is an anaerobic, gram-negative bacterium involved in the so-called "red complex," which is associated with severe and chronic periodontitis. the surface layer (s-layer) of t. forsythia is composed of cell surface glycoproteins, such as tfsa and tfsb, and is known to play a role in adhesion/invasion and suppression of proinflammatory cytokine expression. here we investigated the association of this s-layer with serum resistance and coaggregation with other oral bacteria. the ...201323357386
antibody and t cell responses to fusobacterium nucleatum and treponema denticola in health and chronic periodontitis.the characteristics of the t cell response to the members of oral flora are poorly understood. we characterized the antibody and t cell responses to fada and td92, adhesins from fusobacterium nucleatum, an oral commensal, and treponema denticola, a periodontal pathogen, respectively. peripheral blood and saliva were obtained from healthy individuals and patients with untreated chronic periodontitis (cp, n = 11 paris) and after successful treatment of the disease (n = 9). the levels of antigen-sp ...201323335969
impact of early colonizers on in vitro subgingival biofilm formation.the aim of this study was to investigate the impact of early colonizing species on the structure and the composition of the bacterial community developing in a subgingival 10-species biofilm model system. the model included streptococcus oralis, streptococcus anginosus, actinomycesoris, fusobacterium nucleatum subsp. nucleatum, veillonella dispar, campylobacter rectus, prevotella intermedia, porphyromonas gingivalis, tannerella forsythia, and treponema denticola. based on literature, we consider ...201324340084
analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16s rdna gene sequence.to analyze and characterize the predominant bacterial flora associated with peri-implantitis by using culture techniques under obligate anaerobic conditions and 16s rdna gene sequences.201324278920
a new type of na(+)-driven atp synthase membrane rotor with a two-carboxylate ion-coupling motif.the anaerobic bacterium fusobacterium nucleatum uses glutamate decarboxylation to generate a transmembrane gradient of na⁺. here, we demonstrate that this ion-motive force is directly coupled to atp synthesis, via an f₁f₀-atp synthase with a novel na⁺ recognition motif, shared by other human pathogens. molecular modeling and free-energy simulations of the rotary element of the enzyme, the c-ring, indicate na⁺ specificity in physiological settings. consistently, activity measurements showed na⁺ s ...201323824040
porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of prommp9 and its activation.recent epidemiological studies have revealed a significant association between periodontitis and oral squamous cell carcinoma (oscc). furthermore, matrix metalloproteinase 9 (mmp9) is implicated in the invasion and metastasis of tumour cells. we examined the involvement of porphyromonas gingivalis, a periodontal pathogen, in oscc invasion through induced expression of prommp and its activation. prommp9 was continuously secreted from carcinoma sas cells, while p. gingivalis infection increased pr ...201323991831
association of plasma 25-hydroxyvitamin d concentrations and pathogenic oral bacteria in postmenopausal females.previous findings of an association between 25-hydroxyvitamin d [25(oh)d] concentrations and periodontal disease may be partially explained by the antimicrobial properties of vitamin d. to the best of the authors' knowledge, no study has investigated the association between 25(oh)d and pathogenic oral bacteria, a putative cause of periodontal disease.201324261910
the predominant bacteria isolated from radicular cysts.to detect predominant bacteria associated with radicular cysts and discuss in light of the literature.201324011184
toll-like receptor 2-dependent activity of native major outer membrane protein proteosomes of chlamydia trachomatis.chlamydia trachomatis is the most common sexually transmitted bacterial pathogen and the etiologic agent of blinding trachoma. intracellular signaling pathways leading to host cell inflammation and innate immunity to chlamydia include those mediated by toll-like receptors (tlrs) and nucleotide binding oligomerization domain 1 (nod1) protein. in epithelial cells, tlr-dependent signaling contributes to local immune responses via induction of inflammatory mediators. there is evidence that tlr3, tlr ...201323132491
effects of short-chain fatty acids on actinomyces naeslundii biofilm formation.actinomyces naeslundii is an early colonizer and has important roles in the development of the oral biofilm. short-chain fatty acids (scfa) are secreted extracellularly as a product of metabolism by gram-negative anaerobes, e.g. porphyromonas gingivalis and fusobacterium nucleatum; and the scfa may affect biofilm development with interaction between a. naeslundii and gram-negative bacteria. our aim was to investigate the effects of scfa on biofilm formation by a. naeslundii and to determine the ...201323731652
in vivo study of the initial bacterial adhesion on different implant materials.biofilm formation on implant materials plays a major role in the aetiology of periimplantitis. the aim of this study was to examine in vivo the initial bacterial adhesion on six different implant materials.201323694907
effect of metalloporphyrins on red autofluorescence from oral bacteria.the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ...201323659237
antibacterial activity of probiotic candidates for oral health.the aim of this study was to determine the probiotic potential of autochthonous oral lactobacilli. for this, 66 strains were screened for antibacterial activity against two cariogenic strains (streptococcus mutans and actinomyces viscosus) and two periodontopathogenic strains (fusobacterium nucleatum and porphyromonas gingivalis). the inhibitory activity was investigated with the agar overlay technique. positive results led us to explore some mechanisms of action. the ability to produce h(2)o(2) ...201323211763
characterization and in vitro properties of oral lactobacilli in breastfed infants.lactobacillus species can contribute positively to general and oral health and are frequently acquired by breastfeeding in infancy. the present study aimed to identify oral lactobacilli in breast and formula-fed 4 month-old infants and to evaluate potential probiotic properties of the dominant lactobacillus species detected. saliva and oral swab samples were collected from 133 infants who were enrolled in a longitudinal study (n=240) examining the effect of a new infant formula on child growth a ...201323945215
photodynamic biofilm inactivation by sapyr--an exclusive singlet oxygen photosensitizer.prevention and control of biofilm-growing microorganisms are serious problems in public health due to increasing resistances of some pathogens against antimicrobial drugs and the potential of these microorganisms to cause severe infections in patients. therefore, alternative approaches that are capable of killing pathogens are needed to supplement standard treatment modalities. one alternative is the photodynamic inactivation of bacteria (pib). the lethal effect of pib is based on the principle ...201323891675
microbiological evaluation of bacterial and mycotic seal in implant systems with different implant-abutment interfaces and closing torque values.the aim of this study was to evaluate the possible leakage of 3 species of bacteria (streptococcus sanguinis, fusobacterium nucleatum, and actinomyces odontolyticus) and of candida albicans and candida glabrata in osseointegrated implants with different implant-abutment interface (iai) geometry.201323782848
oral epithelial cell responses to multispecies microbial biofilms.this report describes the use of a novel model of multispecies biofilms to stimulate profiles of cytokines/chemokines from oral epithelial cells that contribute to local inflammation in the periodontium. streptococcus gordonii (sg)/s. oralis (so)/s. sanguinis (ss) and sg/fusobacterium nucleatum (fn)/porphyromonas gingivalis (pg) biofilms elicited significantly elevated levels of il-1α and showed synergistic stimulatory activity compared with an additive effect of the 3 individual bacteria. only ...201323300185
[soldiers in white smocks]. 20133898571
[soldiers in white smocks]. 20133898571
child health status and risk factors. 20133882631
loss of substance of dental composite restorations. 20133861433
dental syndromes in medical genetics. 20133861432
the relationship of movement and time to older adults' functional health.the relationship of movement (measured by walking cadence) and time (measured by perceived duration) to functional health was investigated in a sample of community-based, white, older women (n = 114). significant relationships were found between health and movement, and between movement and time. speed, inherent in both movement and time, may be an important qualitative dimension of functional health. walking cadence shows the most promise for use as an inexpensive, noninvasive, easy-to-use clin ...20133636945
[ventricular fibrillation in the initial phase of acute myocardial infarct].in order to determinate the incidence, predictivity and prognosis of ventricular fibrillation in the early phase of acute myocardial infarction a series of 301 patients with acute myocardial infarction consecutively assisted by the mobile coronary care unit of florence was analyzed. 151 patients (50.2%) received intensive care within 2 hours from the onset of the symptoms, 75 patients (24.9%) received intensive care between the second and sixth hour. 38 patients (12.6%) had at least one episode ...20134007366
[ventricular fibrillation in the initial phase of acute myocardial infarct].in order to determinate the incidence, predictivity and prognosis of ventricular fibrillation in the early phase of acute myocardial infarction a series of 301 patients with acute myocardial infarction consecutively assisted by the mobile coronary care unit of florence was analyzed. 151 patients (50.2%) received intensive care within 2 hours from the onset of the symptoms, 75 patients (24.9%) received intensive care between the second and sixth hour. 38 patients (12.6%) had at least one episode ...20134007366
[biological profile of the 1st 4 hours of a thrombolytic treatment combining urokinase with lysyl-plasminogen].the authors study the biological variations which accompany an oral, thrombolytic treatment in which urokinase and lysyl-plasminogen are combined. fibrinogen, the products of degradation of fibrinogen or fibrin, plasminogen and rapid alpha 2 antiplasmin are studied as a function of time. two dimensional electrophoreses were carried out at precisely determined times. the combination of the two therapeutic agents causes the appearance of plasmin in the circulation, but it is neutralised by its rap ...20133832977
likelihood ratio vs a multivariate model. 20133813825
[patients without vocalizing function. on assistance of alaryngeal patients from the postoperative period to the time of social rehabilitation]. 20133845289
nonhuman primate model for regional wave travel and reflections along aortas.arterial pulse transmission and wave reflections were studied in five mature anesthetized baboons (papio anubis) using multisensor micromanometry. simultaneous pressures were recorded from the left ventricle and every 10 cm along the aorta and its terminal branches, and flow velocity was measured in the aortic root. aortic input impedance and regional foot-to-foot and apparent phase velocities were calculated. aortography provided dimensional data for local reflection coefficients. regional foot ...20133618804
trends in teenage childbearing 1970-83. 20133576441
a specular reflection arising from the ventricular wall: a potential pitfall in the diagnosis of germinal matrix hemorrhage.reflection artifacts may complicate the diagnosis of intracranial hemorrhage in preterm neonates. a linear focus of bright echoes in the region of the germinal matrix at or anterior to the caudothalamic grooves, often seen on parasagittal scans, may be mistaken for a germinal matrix hemorrhage but is actually a reflection of the ultrasound beam at the wall of the lateral ventricle. cranial sonograms of 100 preterm infants were retrospectively analyzed and categorized with regard to presence and ...20133884829
optimization of ultrasonic transducers.the so-called klm-model for ultrasonic transducers is employed to optimize transducer design. some new performance characteristics are defined which change monotonically with design parameters. these characteristics are based on the area of the envelope of the echo waveform produced by the transducer and of the corresponding amplitude spectrum. the efficiency of the transducer is defined by the round trip energy factor. the performance characteristics are used in a composite performance measure, ...20133881869
the midwifery examination system--a matter for concern. 20133634171
[ambulatory mass screening for preventing and improving the outcome of acute pneumonia in military personnel]. 20133576995
a fluid-phase routine method for the detection of insulin-anti-insulin complexes.a fast routine method has been devised to measure circulating insulin-anti-insulin complexes. the principle lies in the calculation of the difference between the insulin binding capacity of the free antibody and that of the total amount of insulin antibody. the ph of 1 aliquot of serum was lowered to 3 by adding glycine-hcl buffer. free insulin was removed by charcoal precipitation and the ph was again neutralized by the simple addition of naoh; the final dilution of serum was 1/5. radiolabelled ...20133816046
[the use of occupational law in solving problems at obstetric-gynecologic facilities]. 20133811411
malignant melanoma arising intradermally in a small congenital nevus of an adult.the occurrence of a malignant melanoma in a small congenital nevus is a rare event. a malignant melanoma arising intradermally in a small congenital nevus in an adult has not been previously reported. the clinical implications and known information about the prognosis of intradermal malignant melanomas are discussed.20133771902
[the lung in cardiopathies]. 20133836616
[outline of the university education on the air: a new type of education for the era of life-long education]. 20133846685
controversies in the actions of digitalis substances: are all digitalis derivatives alike? 20133905878
comparison of the effectiveness of two topical anesthetics and a placebo in reducing injection pain. 20133867659
[limitations of conventional mechanical ventilation in the treatment of acute respiratory failure]. 20133747112
low indoor temperatures and morbidity in the elderly.low ambient temperatures are particularly harmful to the elderly and in the winter in the uk temperatures in some dwellings may fall to 6 degrees c. the world health organization recommends a minimal indoor temperature of 18 degrees c and a 2-3 degrees c warmer minimal temperature for rooms occupied by sedentary elderly, young children and the handicapped. below 16 degrees c, resistance to respiratory infections may be diminished. both low and high relative humidities promote respiratory illness ...20133751747
[possibilities and limits of the study of tumor markers in the after-care of patients with ovarian cancer]. 20133770529
[clinical effects of hyperthermia in patients with carcinoma of the esophagus--a multi-institutional cooperative study]. 20133782986
[on an unusual case of adenocystic carcinoma of the breast]. 20134312699
[on an unusual case of adenocystic carcinoma of the breast]. 20134312699
vitamin and mineral usage in adult family practice patients. 20134056668
patient recall systems. 20133824240
on display. technical innovations and innovators, imaging-related services and educational literature at rsna '87. radiological society of north america. 20133659347
prognosis of alcoholic cirrhosis in the presence and absence of alcoholic hepatitis.liver biopsy specimens (178 percutaneous and 39 transjugular) were assessed from 217 consecutive patients with alcoholic liver disease, 77 noncirrhotics and 140 cirrhotics, whose cases were followed for 5 yr. cirrhotic patients were categorized into two groups, with and without "hepatitis" using a criteria to define "hepatitis" that included only degrees of inflammation, necrosis, and mallory bodies that had a prognostic weight in terms of mortality in 1 yr. this classification resulted in a sha ...20133781189
[sterilization of couples]. 20133639609
[hormone dependence and steroid receptors in adenocarcinomas of the endometrium].the degree of hormone-dependence of endometrial cancers may be evaluated by the presence and the rate of estradiol and mostly progesterone receptors in an endometrial fragment. the most differentiated tumors have a high receptor content, while anaplastic cancers are most of the time without them. they must be systematically determined regardless of other prognostic factors enabling to evaluate the prognosis and guide the treatment. it is presently facilitated by the method of immunochemical titr ...20133602807
the causes and consequences of chronic hepatic venous outflow obstruction.the causes of chronic hepatic venous outflow obstruction are membranous obstruction of the inferior vena cava (group 1), hepatic venous ostial obstruction (group 2) and partial hepatic venous ostial obstruction (group 3). at the h. f. verwoerd and kalafong hospitals, pretoria, 155 cases have been diagnosed, almost all in the first group. over 50% of the patients in this series had hepatocellular carcinoma; this association appears to be a constant finding world-wide. the most likely cause is a c ...20133603285
metastatic disease of the spine. 20133745256
physiologic regulation and pathologic disorders of fibrinolysis.physiologic fibrinolysis is a reparative process that occurs in response to hemostatic plug or thrombus formation. the final enzymatic step, fibrin proteolysis, results from a coordinated interaction of enzymes and inhibitors, which produces effective action at the site of the disease and spares the proteins of the blood or uninvolved parts of the vascular system. the agent of fibrinolysis, the enzyme plasmin, is derived from its zymogen (plasminogen) through limited proteolysis effected by plas ...20133546075
the presidents. gerald desmond timmons 1962-1963. 20133549832
late corneal grafting in congenitally opaque corneas. 20133894096
the structure, function and evolution of cytochromes. 20133881803
combined active and passive immunotherapy in honeybee-sting allergy.five honeybee sting-allergic patients in whom bee venom immunotherapy (vit) had previously been stopped because of repeated allergic side effects (se) were resubmitted to bee vit after pretreatment with beekeeper gammaglobulin. the tolerated bee venom dose was increased five to 800 times after this passive immunotherapy, and the maintenance dose of 100 micrograms of honeybee venom (bv) was reached in all patients after 6 to 15 days of a rush hyposensitization. the igg response to bv was not supp ...20133722631
the tongue. 2. developmental anomalies and coated tongue. 20133861431
sonography of the normal and abnormal intact lumbar spinal canal. 20133880985
sonography of the normal and abnormal intact lumbar spinal canal. 20133880985
diagnosis and treatment of fusobacterium nucleatum discitis and vertebral osteomyelitis: case report and review of the literature.case report.201323132536
prevalence of β-lactamase-producing bacteria in human periodontitis.beta-lactam antibiotics prescribed in periodontal therapy are vulnerable to degradation by bacterial β-lactamases. this study evaluated the occurrence of β-lactamase-positive subgingival bacteria in chronic periodontitis subjects of usa origin, and assessed their in vitro resistance to metronidazole at a breakpoint concentration of 4 μg/ml.201323173872
ascorbate and α-tocopherol differentially modulate reactive oxygen species generation by neutrophils in response to fcγr and tlr agonists.periodontitis, a ubiquitous chronic inflammatory disease, is associated with reduced antioxidant defences and neutrophil hyperactivity in terms of reactive oxygen species (ros) generation. its phenotype is thus characterized by oxidative stress. we have determined the effect of antioxidant micronutrients ascorbate and α-tocopherol on neutrophil ros generation. peripheral neutrophils from periodontally-healthy individuals (n = 20) were challenged with phorbol myristate acetate, igg-opsonised stap ...201322914919
human neutrophil defensins and their effect on epithelial cells.the aims of the present study include: 1) to localize human neutrophil defensin-1 (hnp-1) through hnp-3 in gingiva and in neutrophil extract-treated epithelial cell monolayers; 2) to determine the effects of hnp-1 on the epithelial cell viability, attachment, and spreading; and 3) to analyze the effect of hnp-1 on the bacterial adherence to epithelial cells.201322443519
mycoplasma pneumoniae preceding lemierre's syndrome due to fusobacterium nucleatum complicated by acute epstein-barr virus (ebv) infectious mononucleosis in an immunocompetent host.we report an unusual case of lemierre's syndrome due to a rare species of fusobacterium, that is, fusobacterium nucleatum preceded by mycoplasma pneumoniae pharyngitis and followed later by epstein-barr virus infectious mononucleosis.201322464641
autoinducer 2 of fusobacterium nucleatum as a target molecule to inhibit biofilm formation of periodontopathogens.periodontitis is initiated by bacteria in subgingival biofilms, which are composed mostly of gram-negative anaerobes. autoinducer 2 (ai-2) is a universal quorum sensing (qs) molecule that mediates intergeneric signalling in multispecies bacterial communities and may induce biofilm formation. as fusobacterium nucleatum is the major coaggregation bridge organism that links early colonising commensals and late pathogenic colonisers in dental biofilms via the accretion of periodontopathogens, we hyp ...201322633049
association between oral malodour and periodontal disease-related parameters in the general population.to determine the association between halitosis detection and periodontal status in systemically healthy non-smokers and to assess whether halitosis was related to quantities of fusobacterium nucleatum and porphyromonas gingivalis on the tongue dorsum.201322339235
fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity.sgp1(t), a strain belonging to a lineage of the phylum synergistetes with no previously cultivated representatives was subjected to a comprehensive range of phenotypic and genotypic tests. for good growth the strain was dependent on co-culture with, or extracts from, selected other oral bacteria. cells of strain sgp1(t) were asaccharolytic and major amounts of acetic acid and moderate amounts of propionic acid were produced as end products of metabolism in peptone-yeast extract-glucose broth sup ...201322493171
micronutrient modulation of nf-κb in oral keratinocytes exposed to periodontal bacteria.chronic periodontal diseases are characterised by a dysregulated and exaggerated inflammatory/immune response to plaque bacteria. we have demonstrated previously that oral keratinocytes up-regulate key molecular markers of inflammation, including nf-κb and cytokine signalling, when exposed to the periodontal bacteria porphyromonas gingivalis and fusobacterium nucleatum in vitro. the purpose of the current study was to investigate whether α-lipoic acid was able to abrogate bacterially-induced pro ...201322890546
maternal oral origin of fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16s-23s rrna gene intergenic transcribed spacer region.fusobacterium nucleatum, a common gram-negative anaerobe prevalent in the oral cavity, possesses the ability to colonize the amniotic cavity and the fetus. however, f. nucleatum may also be part of the vaginal microbiota from where it could reach the amniotic tissues. due to the heterogeneity of f. nucleatum, consisting of five subspecies, analysis at the subspecies/strain level is desirable to determine its precise origin. the aims of this study were: (i) to evaluate the use of the 16s-23s rrna ...201323002071
protective potential of non-dialyzable material fraction of cranberry juice on the virulence of p. gingivalis and f. nucleatum mixed infection.periodontitis is a polymicrobial infectious disease. a novel potential chemical treatment modality may lie in bacterial anti-adhesive materials, such as cranberry juice fractions. the aim of this study is to explore the effect of high molecular weight cranberry constituent (non-dialyzable material [ndm]) on the virulence of a mixed infection with porphyromonas gingivalis and fusobacterium nucleatum in mice.201323030239
macrolides inhibit fusobacterium nucleatum-induced muc5ac production in human airway epithelial cells.fusobacterium nucleatum is one of the most common anaerobic bacteria in periodontitis and is responsible for several extraoral infections, including respiratory tract diseases. in this study, we examined whether f. nucleatum induces mucin secretion in airway epithelial cells. we also examined the effects of macrolides on f. nucleatum-induced mucus production compared with the effects of other antibiotics that exert anti-anaerobic activities. the production of muc5ac, the major core protein of mu ...201323380724
characterization of extracellular polymeric matrix, and treatment of fusobacterium nucleatum and porphyromonas gingivalis biofilms with dnase i and proteinase k.biofilms are organized communities of microorganisms embedded in a self-produced extracellular polymeric matrix (epm), often with great phylogenetic variety. bacteria in the subgingival biofilm are key factors that cause periodontal diseases; among these are the gram-negative bacteria fusobacterium nucleatum and porphyromonas gingivalis. the objectives of this study were to characterize the major components of the epm and to test the effect of deoxyribonuclease i (dnase i) and proteinase k.201323372876
characteristics of porphyromonas gingivalis lipopolysaccharide in co-culture with fusobacterium nucleatum.porphyromonas gingivalis is associated with chronic periodontitis and forms multi-species biofilms. they can communicate within species as well as with other species found in the subgingiva, which may induce changes in the growth ratio and virulence of periodontopathogens. the lipopolysaccharide (lps) of p. gingivalis shows different virulence by growth condition. the purpose of this study was to investigate the characteristics of p. gingivalis lps when co-cultured with fusobacterium nucleatum. ...201323347381
clinical and microbiological results following nonsurgical periodontal therapy with or without local administration of piperacillin/tazobactam.we assessed if adjunct administration of piperacillin/tazobactam added clinical and microbiological treatment benefits.201323334268
exploiting preq(1) riboswitches to regulate ribosomal frameshifting.knowing the molecular details of the interaction between riboswitch aptamers and their corresponding metabolites is important to understand gene expression. here we report on a novel in vitro assay to study preq(1) riboswitch aptamers upon binding of 7-aminomethyl-7-deazaguanine (preq(1)). the assay is based on the ability of the preq(1) aptamer to fold, upon ligand binding, into a pseudoknotted structure that is capable of stimulating -1 ribosomal frameshifting (-1 fs). aptamers from three diff ...201323327288
dna binding to hydroxyapatite: a potential mechanism for preservation of microbial dna.molecular methods are increasingly being deployed for analysis of the microbial flora in the root canal. such methods are based on the assumption that recovered dna is associated with the active endodontic infection, yet paleomicrobiology research is based on the recovery of ancient dna from centuries-old tooth and bone samples, which points to considerable longevity of the dna molecule in these tissues. the main component of dentin and bone is the mineral hydroxyapatite. this study assessed dna ...201323321233
isolation and identification of porphyromonas spp. and other putative pathogens from cats with periodontal disease.the purpose of this study was to evaluate the subgingival microbiota and determine the most prevalent periodontal pathogens implicated in feline periodontal disease and to correlate these findings with the clinical periodontal status. subgingival microbiological samples were taken under sedation from 50 cats with clinical signs of periodontal disease. pooled paper point samples from 4 selected subgingival sites were cultured on blood agar and on dentaid-1 medium. suspected pathogens were identif ...201324660305
fusobacterium nucleatum subsp. fusiforme gharbia and shah 1992 is a later synonym of fusobacterium nucleatum subsp. vincentii dzink et al. 1990.on the basis of the dna-dna hybridization patterns and phenotypic characteristics, fusobacterium nucleatum was classified into five subspecies. previous studies have suggested that f. nucleatum subsp. vincentii is genetically similar to f. nucleatum subsp. fusiforme. the aim of this study was to investigate the possibility of classifying these two subspecies into a single subspecies by phylogenetic analysis using a single sequence (24,715 bp) concatenated 22 housekeeping genes of eight f. nuclea ...201323263257
virulence mechanism of bacteria in mixed infection: attenuation of cytokine levels and evasion of polymorphonuclear leukocyte phagocytosis.the objective of the present study is to evaluate the effect of bacterial viability on the virulence of mixed infection.201323259412
suppression of t-cell chemokines by porphyromonas gingivalis.porphyromonas gingivalis is a major pathogen in periodontal disease and is associated with immune dysbiosis. in this study, we found that p. gingivalis did not induce the expression of the t-cell chemokine ip-10 (cxcl10) from neutrophils, peripheral blood mononuclear cells (pbmcs), or gingival epithelial cells. furthermore, p. gingivalis suppressed gamma interferon (ifn-γ)-stimulated release of ip-10, itac (cxcl11), and mig (cxcl9) from epithelial cells and inhibited ip-10 secretion in a mixed i ...201323589576
host susceptibility to periodontitis: mapping murine genomic regions.host susceptibility to periodontal infection is controlled by genetic factors. as a step toward identifying and cloning these factors, we generated an a/j x balb/cj f2 mouse resource population. a genome-wide search for quantitative trait loci (qtl) associated with periodontitis was performed. we aimed to quantify the phenotypic response of the progenies to periodontitis by microct analysis, to perform a genome-wide search for qtl associated with periodontitis, and, finally, to suggest candidate ...201323539559
method to quantify live and dead cells in multi-species oral biofilm by real-time pcr with propidium monoazide.real-time pcr (qpcr) is a widely used technique in analysing environmental and clinical microbiological samples. however, its main limitation was its inability to discriminate between live and dead cells.recently, propidium monoazide (pma) together with qpcr has been used to overcome this problem, with good results for different bacterial species in different types of samples.our objective was to implement this technique for analysing mortality in multi-species oral biofilms formed in vitro with ...201323289803
presence of oral bacterial species in primary endodontic infections of primary teeth.knowledge of the microbial composition of deciduous endodontic infections is limited. this study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions.201324683780
cetylpyridinium chloride suppresses gene expression associated with halitosis.halitosis is a common complaint affecting the majority of the population. mouthrinses containing cetylpyridinium chloride (cpc) have been used as oral hygiene aids to suppress oral malodor. although the clinical efficacy of these mouthrinses has been well-documented, the mechanism whereby cpc reduces malodor is less-well-understood. we hypothesized that cpc suppresses expression of the genes (mgl and cdl) and enzymes responsible for methyl mercaptan (ch3sh) and hydrogen sulfide (h2s) production ...201324112735
differential effect of autoinducer 2 of fusobacterium nucleatum on oral streptococci.autoinducer 2 (ai-2) is a quorum sensing molecule and plays an important role in dental biofilm formation, mediating interspecies communication and virulence expression of oral bacteria. fusobacterium nucleatum connects early colonizing commensals and late colonizing periodontopathogens. f. nucleatum ai-2 and quorum sensing inhibitors (qsis) can manipulate dental biofilm formation. in this study, we evaluated the effect of f. nucleatum ai-2 and qsis on biofilm formation of streptococcus gordonii ...201324112724
myocardial infection due to fusobacterium nucleatum.fusobacterium nucleatum is an anaerobic gram-negative bacillus, which inhabits the oropharynx, gastrointestinal tract, and female genital tract. infections classically affect the head and neck. we report a patient with a myocardial mass due to f. nucleatum, initially thought to be a neoplasm, and discuss anaerobic cardiac infections.201324094834
halitosis vaccines targeting foma, a biofilm-bridging protein of fusobacteria nucleatum.halitosis (bad breath) is estimated to influence more than half of the world's population with varying degree of intensity. more than 85% of halitosis originates from oral bacterial infections. foul-smelling breath mainly results from bacterial production of volatile sulfur compounds (vscs) such as hydrogen sulfide and methyl mercaptan. to date, major treatments for elimination of oral malodor include periodontal therapy combined with antibiotics or antimicrobial agents, and mechanical approache ...201323865430
a periodontal pathogen treponema denticola hijacks the fusobacterium nucleatum-driven host response.periodontitis is a polymicrobial disease that arises from the dysbiosis of the plaque biofilm. to study polymicrobial interactions with gingival epithelial cells, the oral commensal fusobacterium nucleatum and the periodontal pathogen treponema denticola were chosen due to their opposing effects on the expression of human beta-defensins (hbds) and interleukin (il)-8 in gingival epithelial cells. immortalized gingival epithelial hok-16b cells were infected with either f. nucleatum or t. denticola ...201323897119
interactions between lactobacillus crispatus and bacterial vaginosis (bv)-associated bacterial species in initial attachment and biofilm formation.certain anaerobic bacterial species tend to predominate the vaginal flora during bacterial vaginosis (bv), with gardnerella vaginalis being the most common. however, the exact role of g. vaginalis in bv has not yet been determined. the main goal of this study was to test the hypothesis that g. vaginalis is an early colonizer, paving the way for intermediate (e.g., fusobacterium nucleatum) and late colonizers (e.g., prevotella bivia). theoretically, in order to function as an early colonizer, spe ...201323739678
a case of adolescent pelvic inflammatory disease caused by a rare bacterium: fusobacterium nucleatum.pelvic inflammatory disease (pid) is a polymicrobial infection that typically occurs in sexually active females. pid is rare in premenarchal and/or noncoital young women; however, there are case reports of pid occurring in virginal females. we present the first reported case of pid associated with f. nucleatum. in this unusual case of pid in an adolescent, the responsible organism may have originated from the patient's oral cavity.201323619431
meningitis due to fusobacterium nucleatum after a kart crash. 201324471173
necrotizing pneumonia in children: report of 41 cases between 2006 and 2011 in a french tertiary care center.forty-one children hospitalized for necrotizing pneumonia were retrospectively analyzed. necrotizing pneumonia represented 0.8% of community-acquired pneumonia and 6% of hospitalized community-acquired pneumonia. the chest radiograph revealed necrosis on admission in onethird of cases. twenty-one cases (51%) were documented, including 13 staphylococcus aureus, all panton-valentine leukocidin positive, 7 streptococcus pneumoniae and 1 fusobacterium nucleatum.201323722529
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