Publications

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detection of fusobacterium nucleatum in chorionic tissues of high-risk pregnant women.this study was undertaken to investigate the existence of a periodontopathic bacterium, fusobacterium nucleatum, in chorionic tissues of pregnant women, and the effects of f. nucleatum on human chorion-derived cells.201222304677
cytokine expression in response to root canal infection in gnotobiotic mice.to examine cytokine expression profiles during periapical lesion development in response to synergetic human pathogens in a gnotobiotic mouse model.201222233143
gene-diet interactions on colorectal cancer risk.there has been increasing interest lately in understanding how natural dietary antioxidants affect chemoprevention, and recently, there has been a merging of information about antioxidants, endogenous and exogenous reactive oxygen and nitrogen species (rons), and inflammation. rons normally serve the cells as second messengers to regulate many of the intracellular signaling cascades that govern multiple cellular activities. however, when the amount of rons exceeds the cell's ability to metaboliz ...201224392268
[the immune complex]. 20123548652
[the immune complex]. 20123548652
hemangioma of the head and neck. 20123484323
circulating concentrations of 1,25-dihydroxyvitamin d3 in patients with primary hyperparathyroidism.vitamin d metabolism was studied in 65 patients with surgically proven primary hyperparathyroidism. the mean concentration of 1,25-dihydroxyvitamin d3 was 51.7 +/- 34 pg/ml (mean +/- sd) and was not significantly different from normal. renal function was normal in 60 of these patients and in this group circulating 1,25-dihydroxyvitamin d3 was below the lower limit of normal in three and elevated in 17; it was related to the serum concentrations of amino-terminal parathyroid hormone, but was inde ...20123508721
[liver function tests: icg plasma clearance test]. 20123534352
a decade of lower extremity venous trauma: patency and outcome.eighty-two patients with infrailiac venous injuries were reviewed. veins were repaired in 75 patients and ligated in 7 patients. clinical outcome could be correlated with anatomic patency for 41 patients who had postoperative venograms. sixty-three percent of the repairs proved to be patent. simple repairs were successful more often than complex ones. site of injury and perioperative therapeutic adjuncts did not affect results. eleven limbs were amputated, none solely because of venous injury. e ...20123425803
implementing reconsider, a diagnostic prompting computer system, at the georgetown university medical center.reconsider, a computer program for diagnostic prompting developed at the university of california, san francisco, has been implemented at the georgetown university medical center as part of the integrated academic information management system model development grant project supported by the national library of medicine. the system is available for student use in the biomedical information resources center of the dahlgren memorial library. instruction on use of the computer system is provided by ...20123285937
van der waals forces between cylinders. i. nonretarded forces between thin isotropic rods and finite size corrections.the free energy of interaction of two long thin dielectric cylinders is evaluated in the nonretarded limit by the method of van kampen. the result includes all nonadditive many body forces and the effects of an intervening medium. in the limit of dilute systems the result reduces to that obtained by summation of r(-6) pair potentials.20124696762
[a case of focal fatty infiltration of the liver]. 20123534369
[possibilities and limitations of scintigraphy of the parathyroid glands]. 20123317076
amoxicillin. 20124831240
[problems of pharmacotherapy in old age]. 20123295795
understanding the adolescent patient. 20123421853
in vitro evaluation of mtad and nisin in combination against common pathogens associated with root canal infection.many pathogenic microorganisms were found in an infected root canal. the object of this study was to evaluate the effect of mtad in combination with nisin on the pathogens associated with root canal infection.201222414835
subgingival microbiota in adult down syndrome periodontitis.the subgingival microbiota in down syndrome and non-down syndrome adults receiving periodic dental care was examined for 40 bacterial species using checkerboard dna-dna hybridization and the results were related to clinical periodontal attachment loss.201222221039
strain-specific colonization patterns and serum modulation of multi-species oral biofilm development.periodontitis results from an ecological shift in the composition of subgingival biofilms. subgingival community maturation is modulated by inter-organismal interactions and the relationship of communities with the host. in an effort to better understand this process, we evaluated biofilm formation, with oral commensal species, by three strains of the subgingivally prevalent microorganism fusobacterium nucleatum and four strains of the periodontopathogen porphyromonas gingivalis. we also tested ...201222771792
adjunctive azithromycin in the treatment of aggressive periodontitis: microbiological findings of a 12-month randomized clinical trial.to compare the subgingival microbiological outcomes of azithromycin or placebo as adjuncts to scaling and root planing (srp) in the treatment of aggressive periodontitis (agp), and to secondarily evaluate the microbiological effect of supragingival scaling in agp patients.201222445846
osteomyelitis of a long bone due to fusobacterium nucleatum and actinomyces meyeri in an immunocompetent adult: a case report and literature review.fusobacterium species are uncommon causes of osteomyelitis. these organisms are normal flora of the oral cavity. therefore, they mostly cause osteomyelitis of the head and neck. hematogenous osteomyelitis at distant sites other than the head and neck has rarely been reported in pediatric or immunocompromised patients. here, we report the first case of osteomyelitis of a long bone combined with a muscle abscess due to fusobacterium nucleatum in an otherwise healthy adult.201222817336
on being a patient. 20123642262
on being a patient. 20123642262
[analysis of indirect maternal mortality cases].among 145 cases of maternal deaths from the period 1975 to 1983, whose data had been collected in a nationwide survey, 30 cases (21%) were classified as associated causes of maternal deaths. 10 deaths (33%) occurred at the birth of the child, 11 (37%) within 6 weeks after delivery, 4 (13%) within the first trimester and 5 (17%) within the second trimester. the disease that was in causal relation to the complication leading to death was known in 16 cases (53%), unknown in 13 cases (43%); in one c ...20123956963
rheumatoid arthritis of the temporomandibular joint. evaluation of three different radiographic techniques by assessment of observer performance. 20123271696
clonogenic growth and hormone sensitivity of benign and malignant breast tissues. 20123322354
students benefit when counselors, nurses join forces. 20123386193
porphyromonas gingivalis entry into gingival epithelial cells modulated by fusobacterium nucleatum is dependent on lipid rafts.host cell invasion by a major periodontal pathogen, porphyromonas gingivalis, has been proposed as an important mechanism involved in host-pathogen interactions in periodontal and cardiovascular diseases. the present study sought to gain insight into the underlying mechanism(s) involved in previously demonstrated fusobacterial modulation of host cell invasion by p. gingivalis. an immortalized human gingival cell line ca9-22 was dually infected with p. gingivalis atcc 33277 and fusobacterium nucl ...201223034475
antibacterial activity of myristica fragrans against oral pathogens.myristica fragrans houtt is mostly cultivated for spices in penang island, malaysia. the ethyl acetate and ethanol extracts of flesh, mace and seed of myristica fragrans was evaluated the bactericidal potential against three gram-positive cariogenic bacteria (streptococcus mutans atcc 25175, streptococcus mitis atcc 6249, and streptococcus salivarius atcc 13419) and three gram-negative periodontopathic bacteria (aggregatibacter actinomycetemcomitans atcc 29522, porphyromonas gingivalis atcc 3327 ...201223049613
optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens.the aim of this study was to optimize quantitative (real-time) polymerase chain reaction (qpcr) assays for 8 major periodontal pathogens, i.e. aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, parvimonas micros, porphyromonas gingivalis, prevotella intermedia, tanerella forsythia and treponema denticola, and of the caries pathogen streptococcus mutans.201223199017
a proteomic investigation of fusobacterium nucleatum alkaline-induced biofilms.the gram negative anaerobe fusobacterium nucleatum has been implicated in the aetiology of periodontal diseases. although frequently isolated from healthy dental plaque, its numbers and proportion increase in plaque associated with disease. one of the significant physico-chemical changes in the diseased gingival sulcus is increased environmental ph. when grown under controlled conditions in our laboratory, f. nucleatum subspecies polymorphum formed mono-culture biofilms when cultured at ph 8.2. ...201222943491
antibacterial effects of blackberry extract target periodontopathogens.antimicrobial agents provide valuable adjunctive therapy for the prevention and the control of oral diseases. limitations in their prolonged use have stimulated the search for new, naturally occurring agents with more specific activity and fewer adverse effects. here we sought to determine the antibacterial properties of blackberry extract (bbe) in vitro against oral bacterial commensals and periodontopathogens.201222812456
fusobacterium nucleatum and tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model.tannerella forsythia is strongly associated with chronic periodontitis, an inflammatory disease of the tooth-supporting tissues, leading to tooth loss. fusobacterium nucleatum, an opportunistic pathogen, is thought to promote dental plaque formation by serving as a bridge bacterium between early- and late-colonizing species of the oral cavity. previous studies have shown that f. nucleatum species synergize with t. forsythia during biofilm formation and pathogenesis. in the present study, we show ...201222547549
co-localized or randomly distributed? pair cross correlation of in vivo grown subgingival biofilm bacteria quantified by digital image analysis.the polymicrobial nature of periodontal diseases is reflected by the diversity of phylotypes detected in subgingival plaque and the finding that consortia of suspected pathogens rather than single species are associated with disease development. a number of these microorganisms have been demonstrated in vitro to interact and enhance biofilm integration, survival or even pathogenic features. to examine the in vivo relevance of these proposed interactions, we extended the spatial arrangement analy ...201222655057
relationship between oral anaerobic bacteria and otitis media with effusion.in this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (ome), we aimed to investigate the presence and similarity of fusobacterium nucleatum and treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with ome.201222606045
serum antibodies to periodontal pathogens are a risk factor for alzheimer's disease.chronic inflammation in periodontal disease has been suggested as a potential risk factor in alzheimer's disease (ad). the purpose of this study was to examine serum antibody levels to bacteria of periodontal disease in participants who eventually converted to ad compared with the antibody levels in control subjects.201222546352
oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients.oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (hsct). through hsct, the homeostasis in the oral cavity is disrupted. the contribution of the oral microflora to mucositis remains to be clarified. the aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in hsct recipients.201222531876
effect of periodontal pathogens on the metatranscriptome of a healthy multispecies biofilm model.oral bacterial biofilms are highly complex microbial communities with up to 700 different bacterial taxa. we report here the use of metatranscriptomic analysis to study patterns of community gene expression in a multispecies biofilm model composed of species found in healthy oral biofilms (actinomyces naeslundii, lactobacillus casei, streptococcus mitis, veillonella parvula, and fusobacterium nucleatum) and the same biofilm plus the periodontopathogens porphyromonas gingivalis and aggregatibacte ...201222328675
treponema denticola chymotrypsin-like proteinase (ctlp) integrates spirochaetes within oral microbial communities.treponema denticola is found ubiquitously in the human oral cavity and is mainly associated with bacterial communities implicated in the establishment and development of periodontal disease. the ability to become integrated within biofilm communities is crucial to the growth and survival of oral bacteria, and involves inter-bacterial coaggregation, metabolic cooperation, and synergy against host defences. in this article we show that the chymotrypsin-like proteinase (ctlp), found within a high-m ...201222313692
detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women.the aim of this study was to use the fluorescence in situ hybridization (fish) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. this cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque samples were collected and the fish ...201223018230
antibiotic susceptibility profiles of oral pathogens.periodontitis is a bacterial disease that can be treated with systemic antibiotics. the aim of this study was to establish the antibiotic susceptibility profiles of five periodontal pathogens to six commonly used antibiotics in periodontics. a total of 247 periodontal bacterial isolates were tested for susceptibility to the six antibiotics using the etest method. mic(50) and mic(90) values (minimum inhibitory concentrations for 50% and 90% of the organisms, respectively) were calculated. both eu ...201222890195
periodontal pathogen load and increased antibody response to heat shock protein 60 in patients with cardiovascular disease.to determine the relationship between periodontal pathogen load and anti-human heat shock protein 60 (hhsp60) antibodies in patients with established cardiovascular disease (cvd).201222882677
antimicrobial activity of nanoparticulate metal oxides against peri-implantitis pathogens.dental plaque accumulation may result in peri-implantitis, an inflammatory process causing loss of supporting bone that may lead to dental implant failure. the antimicrobial activities of six metal and metal oxide nanoparticles and two of their composites against bacterial pathogens associated with peri-implantitis were examined under anaerobic conditions. the activities of nanoparticles of silver (ag), cuprous oxide (cu(2)o), cupric oxide (cuo), zinc oxide (zno), titanium dioxide (tio(2)), tung ...201222727529
subgingival microbiota from cebus apella (capuchin monkey) with different periodontal conditions.this present study evaluated the subgingival microbiota of the cebus apella with different periodontal conditions kept by the tufted capuchin monkey procreation center (são paulo state university - unesp) or free-ranging monkeys. for this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. the primates were submitted to periodontal evaluation and biofilm samples were transferred to vmga iii transpor ...201222710412
effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis.emingil g, han b, özdemir g, tervahartiala t, vural c, atilla g, baylas h, sorsa t. the effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. j periodont res 2012; 47: 729-739. © 2012 john wiley & sons a/s background and objective:  to study the effectiveness of azithromycin in combination with nonsurgical periodontal therapy on clinical and microbiological parame ...201222571226
quantification of subgingival bacterial pathogens at different stages of periodontal diseases.anaerobic gram-negative oral bacteria such as treponema denticola, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsythia, campylobacter rectus, and fusobacterium nucleatum are closely associated with periodontal diseases. we measured the relative population (bacterial levels) of these oral pathogens in subgingival tissues of patients at different stages of korean chronic periodontal diseases. we divided the individuals into those with chronic gingivitis (g), moder ...201222526568
comparative evaluation of the effects of different photoablative laser irradiation protocols on the gingiva of periodontopathic patients.we aimed at quantifying the presence of periodontopathogens in gingival biopsies from periodontitis patients treated with different photoablative lasers (diode gaas, er:yag, nd:yag, and co(2) lasers) and histologically analyzing their effects on the gingiva.201222401128
[effect of periodontal surgery on the subgingival periodontal microbiota in artificial class iii furcation defects: an experimental study in monkeys].this study was to longitudinally evaluate the change of prevalence of five periodontal putative pathogens in the subgingival plaque of artificial class iii furcation defects at the three time-points, including before the establishment of furcation defects, before and 6 months after periodontal surgery.201222353894
azithromycin as an adjunctive treatment of generalized severe chronic periodontitis: clinical, microbiologic, and biochemical parameters.this study examines the efficacy of azithromycin in combination with non-surgical periodontal therapy on clinical and microbiologic parameters and gingival crevicular fluid (gcf) matrix metalloproteinases-8 (mmp-8) levels over 6 months in patients with severe generalized chronic periodontitis (cp).201222324488
release of metronidazole from electrospun poly(l-lactide-co-d/l-lactide) fibers for local periodontitis treatment.we aimed to achieve detailed biomaterials characterization of a drug delivery system for local periodontitis treatment based on electrospun metronidazole-loaded resorbable polylactide (pla) fibers.201222226009
investigation and quantification of key periodontal pathogens in patients with type 2 diabetes.diabetes is a recognized risk factor for periodontitis. there are conflicting data regarding whether healthy diabetic patients or diabetic patients with chronic periodontitis have an altered subgingival microbiota compared with nondiabetic individuals. the aim of the present study was to detect quantitative differences in selected periodontopathogens in the subgingival plaque of diabetic patients using taqman quantitative pcr.201222220967
reactivation of latent hiv-1 by a wide variety of butyric acid-producing bacteria.latently infected cells harbor human immunodeficiency virus type 1 (hiv-1) proviral dna copies integrated in heterochromatin, allowing persistence of transcriptionally silent proviruses. it is widely accepted that hypoacetylation of histone proteins by histone deacetylases (hdacs) is involved in maintaining the hiv-1 latency by repressing viral transcription. hiv-1 replication can be induced from latently infected cells by environmental factors, such as inflammation and co-infection with other m ...201222322557
a microbiological profile of unexposed and exposed pulp space of primary endodontic infections by checkerboard dna-dna hybridization.the aim of this study was to evaluate, by checkerboard dna-dna hybridization, the composition of the microbiota of primary endodontic infections in cases associated with exposed (n = 30) and unexposed (n = 30) pulp space.201222703649
comprehensive analysis of bacterial flora in postoperative maxillary cyst fluid by 16s rrna gene and culture methods.intracystic fluid was aseptically collected from 11 patients with postoperative maxillary cyst (pomc), and dna was extracted from the pomc fluid. bacterial species were identified by sequencing after cloning of approximately 580 bp of the 16s rrna gene. identification of pathogenic bacteria was also performed by culture methods. the phylogenetic identity was determined by sequencing 517-596 bp in each of the 1139 16s rrna gene clones. a total of 1114 clones were classified while the remaining 25 ...201222685668
microbial diversity in failed endodontic root-filled teeth.persistent/secondary infections of human root canals play an important role in the failure of endodontic treatment. this study used 16s rrna sequencing to assess microbial diversity in root-filled teeth associated with failed endodontic treatment.201222613548
molecular analysis of 16s rrna genes identifies potentially periodontal pathogenic bacteria and archaea in the plaque of partially erupted third molars.small subunit rrna sequencing and phylogenetic analysis were used to identify cultivable and uncultivable microorganisms present in the dental plaque of symptomatic and asymptomatic partially erupted third molars to determine the prevalence of putative periodontal pathogens in pericoronal sites.201222326171
anaerobic and microaerophilic pathogens in the biofilm formation on voice prostheses: a pilot study.voice rehabilitation with voice prostheses is a standard therapy in laryngectomized patients. biofilm formation on the surface of the voice prostheses causes device failure and requires frequent replacements. studies analyzing the biofilm of voice prostheses have mainly focused on aerobic bacteria. anaerobic bacteria as an integral part of the biofilms on voice prostheses have not been investigated yet.201222374743
efficacy of berberine, an antimicrobial plant alkaloid, as an endodontic irrigant against a mixed-culture biofilm in an in vitro tooth model.berberine, a plant alkaloid isolated from many medicinal plants, has shown antimicrobial activity against selected oral pathogens. the purpose of this investigation was to evaluate the antimicrobial efficacy of berberine solution against selected endodontic pathogens using a multispecies biofilm tooth model.201222794217
characterization of microbiota of root canal-treated teeth with posttreatment disease.this study evaluated the microbiota of root canals undergoing retreatment. the most prevalent taxa detected by checkerboard included propionibacterium species, fusobacterium nucleatum, streptococci, and pseudoramibacter alactolyticus. quantitative real-time pcr detected enterococcus faecalis and streptococci in 38% and 41% of the cases, comprising 9.76% and 65.78% of the total bacterial counts, respectively. the findings call into question the status of e. faecalis as the main pathogen and sugge ...201222403423
[lemierre's syndrome as differential diagnosis of lung cancer].lemierre's syndrome is a disseminated infection which is usually caused by fusobacterium necrophorum. an oropharyngeal infection progresses to a septic thrombophlebitis of the internal jugular vein and later metastatic infections throughout the body occur. we present a clinical case in which a patient, initially presenting with symptoms characteristic of pulmonary cancer, turned out to have a rare variant of lemierre's syndrome caused by fusobacterium nucleatum.201222668649
porphyromonas gingivalis mediates inflammasome repression in polymicrobial cultures through a novel mechanism involving reduced endocytosis.the interleukin (il)-1β-processing inflammasome has recently been identified as a target for pathogenic evasion of the inflammatory response by a number of bacteria and viruses. we postulated that the periodontal pathogen, porphyromonas gingivalis may suppress the inflammasome as a mechanism for its low immunogenicity and pathogenic synergy with other, more highly immunogenic periodontal bacteria. our results show that p. gingivalis lacks signaling capability for the activation of the inflammaso ...201222843689
neutrophil superoxide production in the presence of cigarette smoke extract, nicotine and cotinine.to determine the effect of cigarette smoke extract, nicotine and cotinine on lucigenin-detectable neutrophil superoxide production.201222607095
an investigation of the presence of specific anaerobic species in necrotic primary teeth.different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. the aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (pcs) and root canals (rcs) of infected primary teeth using pcr methods. paired pc and rc samples were collected from 15 subjects and analyzed by pcr for the presence of filifactor alocis, fusobacterium nucleatum, parvimonas micra, porphyromonas endod ...201323538426
identification of a 43-kda outer membrane protein of fusobacterium necrophorum that exhibits similarity with pore-forming proteins of other fusobacterium species.a pair of primers was designed in an attempt to amplify outer membrane protein (omp) gene of fusobacterium necrophorum based on nucleotide sequence of the omp of fusobacterium nucleatum. further analysis was performed to characterize its molecular properties and phylogeny in the genus fusobacterium. we identified a predicated 43kda outer membrane protein (43k omp) in f. necrophorum, which showed the same properties as other pore-forming proteins of gram-negative anaerobic bacteria according to a ...201323433684
[evaluation on antimicrobial efficacy of fufang bingpeng irrigant: an in vitro study].to evaluate the antimicrobial efficacy of fufang bingpeng irrigant and bingpeng irrigant on four endodontic pathogen bacteria.201323991570
antibacterial effect of different root canal sealers on three bacterial species.bacteria persisting in the root canal system may thwart endodontic therapy. it is therefore interesting to know whether clinically available root canal sealers have an antimicrobial effect. the objective of the present in vitro study was to investigate the antibacterial effect of various sealers on the endodontologically detectable species enterococcus faecalis (e. faecalis), fusobacterium nucleatum (f. nucleatum) and porphyromonas gingivalis (p. gingivalis).201323523285
oxygen deprivation affects the antimicrobial action of ll-37 as determined by microplate real-time kinetic measurements under anaerobic conditions.some agents, including escherichia coli and group a streptococcus pyogenes cause infections in oxygen depleted sites. ll-37 is a human host defence peptide shown previously to play an important role in controlling infections caused by these bacteria. however, the effect of oxygen levels on the antimicrobial activity of ll-37 remains obscure. in order to test the effect of oxygen (or lack thereof) on ll-37's activity against e. coli and s. pyogenes, a method for adapting commonly used microtiter ...201323664904
the lipid bilayer-inserted membrane protein bama of escherichia coli facilitates insertion and folding of outer membrane protein a from its complex with skp.folding of β-barrel membrane proteins, either from a urea-unfolded form or from chaperone-bound aqueous forms, has been characterized for pure lipid bilayers. the impact of preinserted integral proteins from biomembranes has not been examined in biophysical comparisons, but this knowledge is important for the characterization of protein assembly machinery in membranes to distinguish specific effects from unspecific effects. here, folding was studied for a β-barrel membrane protein, outer membran ...201323641708
comparative microbial analysis of paired amniotic fluid and cord blood from pregnancies complicated by preterm birth and early-onset neonatal sepsis.16s rrna-based genomic analyses have revolutionized our understanding of infectious diseases. many cases which were recognized as "idiopathic" are now known to have an infectious etiology. here, we present a proof-of-concept study to examine the microbial link between intra-amniotic infection (iai) and early-onset neonatal sepsis (eons).201323437088
folding and stability of outer membrane protein a (ompa) from escherichia coli in an amphipathic polymer, amphipol a8-35.amphipols are a class of amphipathic polymers designed to maintain membrane proteins in aqueous solutions in the absence of detergents. denatured β-barrel membrane proteins, like outer membrane proteins ompa from escherichia coli and foma from fusobacterium nucleatum, can be folded by dilution of the denaturant urea in the presence of amphipol a8-35. here, the folding kinetics and stability of ompa in a8-35 have been investigated. folding is well described by two parallel first-order processes, ...201323370791
the effects of stress hormones on growth of selected periodontitis related bacteria.the focus of this study was to examine in vitro the effects of stress hormones (catecholamines: epinephrine, norepinephrine, dopamine and hydrocortisone: cortisol) on the growth of four anaerobic species of periodontitis-related bacteria (fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia and tannerella forsythia) and one facultative anaerobic species (eikenella corrodens). bacterial growth was determined by two different methods: fluorescence in situ hybridization (fish), ...201324036419
subgingival biodiversity in subjects with uncontrolled type-2 diabetes and chronic periodontitis.there is a bidirectional relationship between periodontal disease and type-2 diabetes mellitus (dm). inflammatory mediators may negatively affect glycemic control, and increased glucose levels and resultant glycation end-products may alter the host response against bacterial infection. however, no agreement has been reached regarding the effect of dm on periodontal subgingival microbiota. therefore, the purpose of the present study was to compare the subgingival biodiversity in deep periodontal ...201322762355
advancement in the routine identification of anaerobic bacteria by maldi-tof mass spectrometry.we evaluated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) biotyper as a tool for the identification of anaerobic bacteria compared with 500 base-pair (bp) 16s ribosomal ribonucleic acid (rrna) gene sequencing analysis, which is considered to be the "gold standard" method. a total of 484 anaerobic bacteria were retrieved from the clinical specimens of 318 pediatric patients. molecular identification resulted in 18 genera and 51 species. the most prev ...201323584672
modulation of quaternary structure and enhancement of ligand binding by the k-turn of tandem glycine riboswitches.most known glycine riboswitches have two homologous aptamer domains arranged in tandem and separated by a short linker. the two aptamers associate through reciprocal "quaternary" interactions that have been proposed to result in cooperative glycine binding. recently, the interaptamer linker was found to form helix p0 with a previously unrecognized segment 5' to the first aptamer domain. p0 was shown to increase glycine affinity, abolish cooperativity, and conform to the k-turn motif consensus. w ...201323249744
development of quantitative real-time pcr primers for detecting 42 oral bacterial species.in this study, we introduced species-specific quantitative real-time pcr (qpcr) primers designed based on a dna-dependent rna polymerase beta-subunit gene (rpob) for detecting 42 oral bacterial species. the specificity of the qpcr primers was confirmed by conventional pcr with the genomic dnas of 73-79 strains regarding 73-75 bacterial species including the type strain for the target species. the standard curves revealed the lower detection limits of 42 bacterial species-specific qpcr primers ra ...201323689247
in vitro activity of biapenem plus rpx7009, a carbapenem combined with a serine β-lactamase inhibitor, against anaerobic bacteria.biapenem is a carbapenem being developed in combination with rpx7009, a new inhibitor of serine β-lactamases. biapenem was tested alone and in combination with fixed concentrations of rpx7009 by agar dilution against 377 recent isolates of anaerobes. a separate panel of 27 isolates of bacteroides spp. with decreased susceptibility or resistance to imipenem was also tested. comparator drugs included meropenem, piperacillin-tazobactam, ampicillin-sulbactam, cefoxitin, ceftazidime, metronidazole, c ...201323529731
antibacterial activity of probiotic candidates for oral health.the aim of this study was to determine the probiotic potential of autochthonous oral lactobacilli. for this, 66 strains were screened for antibacterial activity against two cariogenic strains (streptococcus mutans and actinomyces viscosus) and two periodontopathogenic strains (fusobacterium nucleatum and porphyromonas gingivalis). the inhibitory activity was investigated with the agar overlay technique. positive results led us to explore some mechanisms of action. the ability to produce h(2)o(2) ...201323211763
adhesion of aggregatibacter actinomycetemcomitans and fusobacterium nucleatum on bioactive tio(2) surfaces.bioactive tio(2) coatings have been found to enhance fibroblast adhesion and gingival attachment on the titanium surfaces, but no information is available whether the coatings also promote the adhesion of periodontal pathogens.201322220677
the antibacterial activity of various saturated and unsaturated fatty acids against several oral pathogens.the antibacterial activity of various saturated fatty acids (sfa) and unsaturated fatty acids (usfa) against different oral pathogens which are implicated in the cause of dental caries, stomatitis, gingivitis, and periodontitis was examined. the saturated fatty acids pa, sta and ara, and the unsaturated omega-7 fatty acids pla and omega-9 fatty acids oa showed either none to low antimicrobial activity against all of the 12 oral pathogenic strains used in this study. in contrast, the omega-3 pufa ...201324640241
specificity of antimicrobial peptide ll-37 to neutralize periodontopathogenic lipopolysaccharide activity in human oral fibroblasts.the antimicrobial peptide ll-37 is known to have a potent lipopolysaccharide (lps)-neutralizing activity in various cell types. because of observed heterogeneity within periodontopathogenic lps, the authors hypothesized that ll-37 had specificity to neutralize such lps activity. the present study, therefore, aims to investigate the lps-neutralizing activity of ll-37 to various periodontopathogenic lps in interleukin-8 (il-8) production after challenging them in human oral fibroblasts.201322443521
bacterial antagonism against periodontopathogens.the aim of the current study is to compare the prevalence of commensal bacteria, with beneficial properties, for healthy and diseased individuals and additionally to examine the inhibitory effect of some commercial dietary probiotics on periodontopathogens, comparing this inhibitory effect to that of orally derived beneficial bacteria.201322897652
peptides: β-cyclodextrin inclusion compounds as highly effective antimicrobial and anti-epithelial proliferation agents.the use of antimicrobial peptides (amps) as therapeutic agents for periodontal infections has great advantages, such as broad spectrum of action, low toxicity, and limited bacterial resistance. however, their practical use is limited because of the large amount of peptide required to exercise the microbicidal function.201323510146
in vitro antimicrobial effects of two antihalitosis mouth rinses on oral pathogens and human tongue microbiota.the aim of the study was to compare the antimicrobial activity of a mouth rinse containing chlorhexidine and cetylpyridinium chloride (mr1) with a stannous fluoride-based mouth rinse (mr2) in vitro.201323368885
anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.human mesenchymal stem cells (hmscs) are multipotent by nature and are originally isolated from bone marrow. in light of a future application of hmscs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacterial species i.e. periodontitis pathogens, we performed this study to gain information about the behavior of hmsc in an anaerobic system and the response in interaction with oral bacterial pathogens.201324223777
comparative evaluation of subgingival plaque microflora in pregnant and non-pregnant women: a clinical and microbiologic study.gingival changes in pregnancy have been attributed to changes in the subgingival biofilm related to hormonal variations.201323633772
oral community interactions of filifactor alocis in vitro.filifactor alocis is a gram positive anaerobe that is emerging as an important periodontal pathogen. in the oral cavity f. alocis colonizes polymicrobial biofilm communities; however, little is known regarding the nature of the interactions between f. alocis and other oral biofilm bacteria. here we investigate the community interactions of two strains of f. alocis with streptococcus gordonii, fusobacterium nucleatum, porphyromonas gingivalis and aggregatibacter actinomycetemcomitans, organisms w ...201324098460
antimicrobial activity of isothiocyanates (itcs) extracted from horseradish (armoracia rusticana) root against oral microorganisms.the antimicrobial activity of isothiocyanates (itcs) extracted from horseradish root was investigated against oral microorganisms: 6 strains of facultative anaerobic bacteria, streptococcus mutans, streptococcus sobrinus, lactobacillus casei, staphylococcus aureus, enterococcus faecalis and aggregatibacter actinomycetemcomitans; one strain of yeast, candida albicans, and 3 strains of anaerobic bacteria, fusobacterium nucleatum, prevotella nigrescens, and clostridium perfringens. the itcs extract ...201324077540
salivary detection of periodontopathic bacteria in fanconi's anemia patients.fanconi's anemia (fa) is characterized by bone marrow failure and can lead to infections such as periodontal disease. the aim of this study was to compare the prevalence of four periodontopathic bacteria including aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum and treponema denticola in saliva samples from children with and without fa. paraffin-stimulated saliva samples were collected from 71 children and adolescents, aged 6-18 years old. the samples wer ...201324055631
the connection between ruptured cerebral aneurysms and odontogenic bacteria.patients with ruptured saccular intracranial aneurysms have excess long-term mortality due to cerebrovascular and cardiovascular diseases compared with general population. chronic inflammation is detected in ruptured intracranial aneurysms, abdominal aortic aneurysms and coronary artery plaques. bacterial infections have been suggested to have a role in the aetiology of atherosclerosis. bacteria have been detected both in abdominal and coronary arteries but their presence in intracranial aneurys ...201323761916
porphyromonas gingivalis, treponema denticola and toll-like receptor 2 are associated with hypertensive disorders in placental tissue: a case-control study.to explore the associations between the presence of periodontal pathogens and the expression of toll-like receptors (tlr-2 and tlr-4) in the placental tissue of patients with hypertensive disorders compared to the placentas of healthy normotensive patients.201323711357
phototoxic effect of blue light on the planktonic and biofilm state of anaerobic periodontal pathogens.the purpose of this study was to compare the phototoxic effects of blue light exposure on periodontal pathogens in both planktonic and biofilm cultures.201323678390
impact of early colonizers on in vitro subgingival biofilm formation.the aim of this study was to investigate the impact of early colonizing species on the structure and the composition of the bacterial community developing in a subgingival 10-species biofilm model system. the model included streptococcus oralis, streptococcus anginosus, actinomycesoris, fusobacterium nucleatum subsp. nucleatum, veillonella dispar, campylobacter rectus, prevotella intermedia, porphyromonas gingivalis, tannerella forsythia, and treponema denticola. based on literature, we consider ...201324340084
effect of controlled-release periochip™ on clinical and microbiological parameters in patients of chronic periodontitis.the aim of the present study was to evaluate and compare the clinical and microbiological effectiveness of periochip™ as an adjunct to scaling and root planing (srp) with srp alone in patients with chronic periodontitis.201324174754
quantitative analysis of periodontal pathogens present in the saliva of geriatric subjects.at present, information regarding periodontal disease in geriatric patients is scarce. the purpose of this study was to quantify the periodontal pathogens present in the saliva of korean geriatric patients and assess the relationship between the bacterial levels and the periodontal condition.201324040571
bacterial community development in experimental gingivitis.current knowledge of the microbial composition of dental plaque in early gingivitis is based largely on microscopy and cultural methods, which do not provide a comprehensive description of oral microbial communities. this study used 454-pyrosequencing of the v1-v3 region of 16s rrna genes (approximately 500 bp), and bacterial culture, to characterize the composition of plaque during the transition from periodontal health to gingivitis. a total of 20 healthy volunteers abstained from oral hygiene ...201323967169
mobile microbiome: oral bacteria in extra-oral infections and inflammation.the link between oral infections and adverse systemic conditions has attracted much attention in the research community. several mechanisms have been proposed, including spread of the oral infection due to transient bacteremia resulting in bacterial colonization in extra-oral sites, systemic injury by free toxins of oral pathogens, and systemic inflammation caused by soluble antigens of oral pathogens. mounting evidence supports a major role of the systemic spread of oral commensals and pathogen ...201323625375
short-term microbiological effects of scaling and root planing and essential-oils mouthwash in chinese adults.to assess the short-term effect of scaling and root planing (srp) and essential-oils mouthwash on the levels of specific bacteria in chinese adults.201323645178
the surface layer of tannerella forsythia contributes to serum resistance and oral bacterial coaggregation.tannerella forsythia is an anaerobic, gram-negative bacterium involved in the so-called "red complex," which is associated with severe and chronic periodontitis. the surface layer (s-layer) of t. forsythia is composed of cell surface glycoproteins, such as tfsa and tfsb, and is known to play a role in adhesion/invasion and suppression of proinflammatory cytokine expression. here we investigated the association of this s-layer with serum resistance and coaggregation with other oral bacteria. the ...201323357386
antibody and t cell responses to fusobacterium nucleatum and treponema denticola in health and chronic periodontitis.the characteristics of the t cell response to the members of oral flora are poorly understood. we characterized the antibody and t cell responses to fada and td92, adhesins from fusobacterium nucleatum, an oral commensal, and treponema denticola, a periodontal pathogen, respectively. peripheral blood and saliva were obtained from healthy individuals and patients with untreated chronic periodontitis (cp, n = 11 paris) and after successful treatment of the disease (n = 9). the levels of antigen-sp ...201323335969
association of plasma 25-hydroxyvitamin d concentrations and pathogenic oral bacteria in postmenopausal females.previous findings of an association between 25-hydroxyvitamin d [25(oh)d] concentrations and periodontal disease may be partially explained by the antimicrobial properties of vitamin d. to the best of the authors' knowledge, no study has investigated the association between 25(oh)d and pathogenic oral bacteria, a putative cause of periodontal disease.201324261910
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