Publications

second stage in the war against asbestos is about to be unleashed.		2011	3173953
second stage in the war against asbestos is about to be unleashed.		2011	3173953
esthetic partial coverage restorations.		2011	3248246
[andrological abnormalities in internal diseases and following drug therapy].	in the present paper the following non-endocrine internal diseases are discussed: liver cirrhosis, diabetes, chronic renal failure and morbus crohn. in alcoholic liver patients under fifty, hypospermia and oligozoospermia can be observed. the hormone assays showed moderately increased fsh- and lh-values in the serum; prolactin, testosterone and estradiol remained normal. an increased binding of testosterone to shbg is supposed, and the androgen deficiency symptoms are considered to be due to the ...	2011	3115148
long ischaemic stricture of the interposed colon.	a case is reported of a long segment stricture in a colonic oesophageal replacement with radiographic evidence to support the diagnosis of ischaemia. ischaemia of the substitute organ following oesophageal resection is a well recognised, serious complication that usually results in anastomotic leakage. if the anastomosis heals primarily, late manifestations of ischaemia are rare.	2011	3206389
[skeletal changes following long-term treatment with retinoids].	the synthetic retinoids, the vitamin-d-derivatives etretinate and isotretinoin, have substantially enlarged the therapeutic arsenal in dermatology. they are primarily used in severe cases of acne and cornification disorders. in the majority of cases, long-term treatment is necessary. certain side effects in the skeletal system can occur, e.g., osteoporosis, premature epiphyseal closure, and changes similar to dish (diffuse idiopathic skeletal hyperostosis). we discuss the reports in the literatu ...	2011	3045876
gas chromatographic method for simultaneous analysis of urinary testosterone, androsterone and etiocholanolone. determinations after a single dose of testosterone.		2011	3237733
ganglion cells in the turtle retina contain the neuropeptide lant-6.	this study investigated the presence of the neurotensin-related hexapeptide, lant-6, in retinal ganglion cells and their central projections in the turtle pseudemys scripta elegans. immunocytochemical techniques demonstrated that many of the cells in the ganglion cell layer of the turtle retina could be labeled with an antiserum specific for lant-6. radioimmunoassay and chromatographic analysis confirmed the presence of lant-6-related peptides in retina, as well as brain. several molecular forms ...	2011	3339403
hmg coa reductase inhibitors. current clinical experience.	lovastatin and simvastatin are the 2 best-known members of the class of hypolipidaemic agents known as hmg coa reductase inhibitors. clinical experience with lovastatin includes over 5000 patients, 700 of whom have been treated for 2 years or more, and experience with simvastatin includes over 3500 patients, of whom 350 have been treated for 18 months or more. lovastatin has been marketed in the united states for over 6 months. both agents show substantial clinical efficacy, with reductions in t ...	2011	3076126
[cytostatic treatment of pseudomyxoma peritonei].	clinic, pathomorphology and therapy of pseudomyxoma peritonei are explained and discussed by means of a women of 54 years. the prognosis in former times very bad could be improved by a combination of radical surgery and polychemotherapy.	2011	3004068
[kinetic studies on indium-111-oxine labelled lymphocytes from patients with chronic lymphocytic leukemia].		2011	3094311
fructosamine concentrations in the serum of diabetics and healthy individuals in singapore.		2011	3252462
fructosamine concentrations in the serum of diabetics and healthy individuals in singapore.		2011	3252462
the role of acidification in the inhibition of neisseria gonorrhoeae by vaginal lactobacilli during anaerobic growth.	vaginal lactobacilli protect the female genital tract by producing lactic acid, bacteriocins, hydrogen peroxide or a local immune response. in bacterial vaginosis, normal lactobacilli are replaced by an anaerobic flora and this may increase susceptibility to neisseria gonorrhoeae, a facultative anaerobe. bacterial interference between vaginal lactobacilli and n. gonorrhoeae has not been studied in liquid medium under anaerobic conditions. by co-cultivating n. gonorrhoeae in the presence of lacto ...	2011	21329492
fusobacterium nucleatum in periodontal health and disease.	the pathogenesis of periodontitis involves the interplay of microbiota present in the subgingival plaque and the host responses. inflammation and destruction of periodontal tissues are considered to result from the response of a susceptible host to a microbial biofilm containing gram-negative pathogens. antimicrobial peptides are important contributors to maintaining the balance between health and disease in this complex environment. these include several salivary antimicrobial peptides such as ...	2011	21220789
epigenetic regulation of human β-defensin 2 and cc chemokine ligand 20 expression in gingival epithelial cells in response to oral bacteria.	gingival epithelia utilize multiple signaling pathways to regulate innate immune responses to various oral bacteria, but little is understood about how these bacteria alter epithelial epigenetic status. in this study we report that dna methyltransferase (dnmt1) and histone deacetylase expression were decreased in gingival epithelial cells treated with oral pathogen porphyromonas gingivalis and nonpathogen fusobacterium nucleatum. pretreatment with trichostatin a and sodium butyrate, which increa ...	2011	21248725
effect of smoking on subgingival microflora of patients with periodontitis in japan.	smoking is a risk factor for periodontitis. to clarify the contribution of smoking to periodontitis, it is essential to assess the relationship between smoking and the subgingival microflora. the aim of this study was to gain an insight into the influence of smoking on the microflora of japanese patients with periodontitis.	2011	21208407
identification of periodontal pathogens and severity of periodontitis in patients with and without chronic kidney disease.	in this study of patients with chronic periodontitis (cp), the severity of the disease and the main periodontal pathogens identified in patients with chronic kidney disease (ckd) were compared with those detected in individuals without systemic disease.	2011	21211789
effect of nicotine, cotinine and cigarette smoke extract on the neutrophil respiratory burst.	to determine the effect of nicotine, cotinine and cigarette smoke extract (cse) on the neutrophil respiratory burst and their effect on activation of the nuclear factor-κb (nfκb) pathway in oral epithelium.	2011	21214612
antimicrobial activity of diterpenes from viguiera arenaria against endodontic bacteria.	six pimarane-type diterpenes isolated from viguiera arenaria baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. the microdilution method was used for the determination of the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) against porphyromonas gingivalis, prevotella nigrescens, prevotella intermedia, prevotella buccae, fusobacterium nucleatum, bacteroi ...	2011	21233793
invasive potential of gut mucosa-derived fusobacterium nucleatum positively correlates with ibd status of the host.	background:: fusobacterium nucleatum is a heterogeneous oral pathogen that is also a common resident of the human gut mucosa. given that some strains of f. nucleatum are known to be invasive and proinflammatory in the oral mucosa, we compared strains isolated from patients with inflammatory bowel disease (ibd) with strains isolated from healthy controls to determine 1) whether this species was more commonly associated with ibd patients; and 2) whether gut-derived f. nucleatum strains from ibd pa ...	2011	21234912
[fusobacterium nucleatum isolated from a patient presenting lachrymal canaliculitis].	a 68-year-old woman presented with a painless inflammation of the right superior eyelid that had started several weeks before. the clinical diagnosis concluded in canaliculitis and the solid concretions were surgically extracted from the superior canalicula. the anaerobic bacteria fusobacterium nucleatum sp. nucleatum was isolated. signs dramatically regressed two weeks after surgery followed by one course of oral amoxicillin and clavulanic acid associated with topical tobramycin. the clinical s ...	2011	21255867
[a case of liver abscess caused by fusobacterium nucleatum in a patient with recurrent periodontal diseases].	fusobacteria are anaerobic gram-negative, non-spore forming bacilli found in normal flora of the oral cavity, urogenital tract, and gastrointestinal tract. fusobacterium nucleatum has been seldom reported as a cause of liver abscess, particularly in immunocompetent hosts. a 55-year-old man with frequent periodontal disease visited our hospital with intermittent fever and headache for 2 months. abdominal ct scan revealed an 8.2 × 6 cm mass in the right hepatic lobe with central low density. absce ...	2011	21258201
salivary infectious agents and periodontal disease status.	the potential of salivary microorganisms to diagnose periodontal disease and to guide periodontal treatment is a research topic of current interest. this study aimed to determine whether the salivary counts of periodontopathic microbes correlated with the periodontal pocket counts of the same infectious agents, and whether the salivary counts of the test infectious agents could distinguish among individuals with periodontal health and various types of periodontal disease.	2011	21261620
structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota.	there are few in vitro models available in the scientific literature for study of the structure, formation and development of the subgingival biofilm. the purpose of this study was to develop and validate an in vitro biofilm model, using representative selected bacteria from the subgingival microbiota.	2011	21261622
acute appendicitis is characterised by local invasion with fusobacterium nucleatum/necrophorum.	acute appendicitis is a local intestinal inflammation with unclear origin. the aim was to test whether bacteria in appendicitis differ in composition to bacteria found in caecal biopsies from healthy and disease controls.	2011	19926616
a 6-month study of the effects of 0.3% triclosan/copolymer dentifrice on dental implants.	supportive therapy to maintain dental implants is increasingly important. this study examined the effect of a 0.3% triclosan/2% copolymer dentifrice on oral biofilms and gingival inflammation (gi) on dental implants and peri-implant tissues.	2011	20831669
fusobacterium nucleatum binding to complement regulatory protein cd46 modulates the expression and secretion of cytokines and matrix metalloproteinases by oral epithelial cells.	periodontitis is a chronic inflammatory disease that results in the destruction of the supporting tissues of the teeth. gingival epithelial cells are an important mechanical barrier and participate in the host inflammatory response to periodontopathogens. the aim of the present study is to investigate the capacity of fusobacterium nucleatum to bind to the complement regulatory protein cd46 expressed by oral epithelial cells and to determine the impact of the binding on the gene expression and pr ...	2011	20843232
fusobacterium nucleatum regulation of neutrophil transcription.	abnormal neutrophil responses have been observed in periodontitis patients, including hyper-reactivity in terms of production of reactive oxygen species (ros) following exposure to the key quorum-sensing plaque bacterium, fusobacterium nucleatum. this study was designed to characterize the transcriptional response of neutrophils to f. nucleatum.	2011	20663022
polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells.	to investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (dcs), at the mucosal surface.	2011	20798974
antiaggregation potential of berry fractions against pairs of streptococcus mutans with fusobacterium nucleatum or actinomyces naeslundii.	coaggregation is an interspecies adhesion process, which is essential to the development of dental plaque. this is an in vitro study of the composition of the soluble solids in the berry juice molecular size fractions (<10 kda, fi; 10-100 kda, fii; >100 kda, fiii) derived from apple, bilberry, blackcurrant, cloudberry, crowberry and lingonberry and their ability to inhibit and reverse coaggregation of the pairs of common species in dental plaque: streptococcus mutans with fusobacterium nucleatum ...	2011	20623601
spiramycin resistance in human periodontitis microbiota.	purpose: the occurrence of in vitro resistance to therapeutic concentrations of spiramycin, amoxicillin, and metronidazole was determined for putative periodontal pathogens isolated in the united states. materials and methods: subgingival plaque specimens from 37 consecutive adults with untreated severe periodontitis were anaerobically cultured, and isolated putative periodontal pathogens were identified to a species level. in vitro resistance to spiramycin at 4 μg/ml, amoxicillin at 8 μg/ml, an ...	2011	21524712
fusobacterium nucleatum infection of colonic cells stimulates muc2 mucin and tumor necrosis factor-{alpha}	even though the etiology of inflammatory bowel disease is not completely known, it is influenced by the presence of normal gut microflora as well as-yet, unrecognized pathogens. the anaerobic, gram-negative bacterial species, fusobacterium nucleatum, is a common resident of the human mouth and gut and varies in its pathogenic potential. in this study, we demonstrate that highly invasive f. nucleatum isolates derived from the inflamed gut of crohn's disease patients evoked significantly greater m ...	2011	21536792
inhibitory effect of lactobacillus reuteri on periodontopathic and cariogenic bacteria.	the interaction between lactobacillus reuteri, a probiotic bacterium, and oral pathogenic bacteria have not been studied adequately. this study examined the effects of l. reuteri on the proliferation of periodontopathic bacteria including aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, and tannerella forsythia, and on the formation of streptococcus mutans biofilms. human-derived l. reuteri strains (kctc 3594 and kctc 3678) and rat-derived l. reuteri kctc ...	2011	21538238
interaction of oral bacteria with gingival epithelial cell multilayers.	primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. multilayers maintained at an air-liquid interface in low-calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. multilayers were infected with fluorescently labeled porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, fusobacterium nucleatum or streptococcus gord ...	2011	21545698
antibacterial compounds from siraitia grosvenorii leaves.	luo han guo (lhg) fruits (siraitia grosvenorii swingle) have been used as traditional medicine in china for centuries to treat sore throats and coughs. however, lhg leaves are seldom used and minimal scientific information is available on them. in our recent study on the leaves of s. grosvenorii, the bioactive compounds β-amyrin (2), aloe emodin (5), aloe-emodin acetate (6), 5α,8α-epidioxy-24(r)-methylcholesta-6,22-dien-3β-ol (7) and p-hydroxyl benzyl acid (8), accompanied by n-hexadecaoic acid ...	2011	21547839
garlic allicin as a potential agent for controlling oral pathogens.	abstract garlic has been used medicinally throughout human history. allicin is considered the most therapeutic constituent of garlic. this study tested the antimicrobial activity of garlic allicin on oral pathogens associated with dental caries and periodontitis. allicin was found effective against all the tested bacteria. the broth dilution method revealed that planktonic growth of the cariogenic, gram-positive species streptococcus mutans, s. sobrinus, and actinomyces oris was inhibited by an ...	2011	21548800
oxidative stress response in the opportunistic oral pathogen fusobacterium nucleatum.	the anaerobic, gram-negative bacillus fusobacterium nucleatum plays a vital role in oral biofilm formation and the development of periodontal disease. the organism plays a central bridging role between early and late colonizers within dental plaque and plays a protective role against reactive oxygen species. using a two-dimensional gel electrophoresis and mass spectrometry approach, we have annotated 78 proteins within the proteome of f. nucleatum subsp. nucleatum and identified those proteins w ...	2011	21563313
antimicrobial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study.	becerik s, türkoğlu o, emingil g, vural c, özdemir g, atilla g. anti-microbial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study. apmis 2011; 119: 364-72. the aim of this study was to examine the effectiveness of chlorhexidine mouthrinse (chx) in addition to daily plaque control on subgingival microbiota in patients with untreated gingivitis. fifty gingivitis patients were randomized to chx or placebo groups. chx group rinsed wit ...	2011	21569094
characteristics and outcomes of fusobacterium nucleatum bacteremia-a 6-year experience at a tertiary care hospital in northern taiwan.	fusobacterium nucleatum bacteremia is critical and not well defined. to identify the clinical characteristics and outcomes, we conducted a retrospective review of hospitalized patients from january 2004 to december 2009 at a tertiary center in northern taiwan. fifty-seven patients were enrolled. the mean age was 58.1 years, and the mean pitt bacteremia score was 4.7. males predominated (59.6%), and the overall 30-day mortality rate was up to 47.4%. malignancy was the major comorbidity (26/57, 45 ...	2011	21596220
[weight loss and dyspnea in a 46-year-old woman.]		2011	21601965
evaluation of three swab transport systems for the maintenance of clinically important bacteria in simulated mono- and polymicrobial samples.	in this study, three swab transport systems were evaluated: m40 transystem, amies broth with a relatively new type of swab (both copan diagnostics, corona, ca, usa), and ssi transportmedium (statens serum institut, copenhagen denmark). the clsi m40-a standard procedures and 11 culture collection strains were used. the transport systems were tested at room temperature for holding times of 0, 24, and 48 h, and both mono- and polymicrobial samples were included. after 24 h of simulated transportati ...	2011	21284737
multispecies communities: interspecies interactions influence growth on saliva as sole nutritional source.	human oral bacteria live in multispecies communities in the biofilm called dental plaque. this review focuses on the interactions of seven species and the ability of each species individually and together with other species to grow on saliva as the sole source of nutrient. community formation in biofilms in flow cells is monitored using species-specific fluorophore-conjugated immunoglobulin g, and images are captured by confocal microscopy. early colonizing veillonellae emerge from this review o ...	2011	21485308
analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis profiling.	information on co-adherence of different oral bacterial species is important for understanding interspecies interactions within oral microbial community. current knowledge on this topic is heavily based on pariwise coaggregation of known, cultivable species. in this study, we employed a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) to systematically analyze the co-adherence profiles of oral bacterial species, and achieved a more ...	2011	21485313
microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease.	uzel ng, teles fr, teles rp, song xq, torresyap g, socransky ss, haffajee ad. microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. j clin peridontol 2011; doi: 10.1111/j.1600-051x.2011.01730.x. abstract: aim: to monitor microbial shifts during dental biofilm re-development. materials and methods: supra- and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects at baseline and i ...	2011	21488936
influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro.	objectives: the aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (ti) substrates on initial oral biofilm formation. materials and methods: nine bacterial species belonging to the normal oral microbiota, including: aggregatibacter actinomycetemcomitans, actinomyces israelii, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, parvimonas micra, porphyromonas gingivalis, prevotella intermedia, and streptococcus sanguinis were teste ...	2011	21492236
identification and enzymatic analysis of a novel protein associated with production of hydrogen sulfide and l-serine from l-cysteine in fusobacterium nucleatum subsp. nucleatum atcc 25586.	a third enzyme that produced hydrogen sulfide from l-cysteine was identified in fusobacterium nucleatum subsp. nucleatum. the fn1055 gene was cloned from a cosmid library constructed with the genomic dna of f. nucleatum atcc 25586. despite the database annotation that the product of fn1055 is a cysteine synthase, reverse phase hplc revealed that no l-cysteine was produced in vitro by the purified fn1055 protein; however, the enzyme did produce l-serine. in addition, a cysteine auxotroph, escheri ...	2011	21493682
effect of ozone on periodontopathogenic species-an in vitro study.	the in vitro study was aimed to determine the effect of ozone on periodontopathogenic microorganisms. ozone was generated for 6 s-2?×?24 s (corresponding to 0.56 mg-2?×?2.24 mg of ozone) against 23 mainly anaerobic periodontopathogenic species. agar diffusion test was used as a screening method. then, the killing activity was tested in a serum-free environment and with 25% v/v inactivated serum. further, the effect of ozone on bactericidal activity of native serum was analyzed against fusobacter ...	2011	21287208
streptococcus cristatus modulates the fusobacterium nucleatum-induced epithelial interleukin-8 response through the nuclear factor-kappa b pathway.	zhang g, chen r, rudney jd.streptococcus cristatusmodulates thefusobacterium nucleatum-induced epithelial interleukin-8 response through the nuclear factor-kappa b pathway. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01373.x.©2011 john wiley & sons a/s background and objective: we previously reported that the interleukin-8 (il-8) response to fusobacterum nucleatum was attenuated in the presence of streptococcus cristatus. here, we further examined the underlying mechanism(s) involved in ...	2011	21521225
experimental alveolitis in rats: microbiological, acute phase response and histometric characterization of delayed alveolar healing.	the pathogenesis of alveolitis is not well known and therefore experimental situations that mimic some features of this disease should be developed.	2011	21625744
identification of an l-methionine {gamma}-lyase involved in the production of hydrogen sulfide from l-cysteine in fusobacterium nucleatum subsp. nucleatum atcc 25586.	fusobacterium nucleatum produces an abundance of hydrogen sulfide (h(2)s) in the oral cavity, which is mediated by several enzymes. the identification and characterization of three distinct enzymes (fn0625, fn1055 and fn1220) in f. nucleatum that catalyze the production of h(2)s from l-cysteine has been reported. in the current study, a novel enzyme involved in the production of h(2)s in f. nucleatum atcc 25586, whose molecular mass had been estimated to be approximately 130-kda, was identified ...	2011	21798982
microbiological aspects of human mandibular subperiosteal dental implants.	abstract purpose: clinical, microbiological and biochemical features of human mandibular subperiosteal dental implants exhibiting peri-implantitis were compared to those experiencing long-term peri-implant health.materials and methods: after evaluation of clinical parameters, submucosal plaque biofilm samples were obtained from permucosal implant abutment posts exhibiting probing depths = 5 mm and bleeding on probing in subjects with peri-implantitis (n = 3), and from posts with peri-implant hea ...	2011	21767207
analysis of the antimicrobial susceptibility of anaerobic bacteria isolated from endodontic infections in brazil during a period of nine years.	the purpose of this study was to analyze the susceptibility of some anaerobic species isolated from a brazilian population at different periods of time by determining a pattern of development of resistance to frequently prescribed antibiotics in endodontics.	2011	21763894
molecular sensing by the aptamer domain of the fmn riboswitch: a general model for ligand binding by conformational selection.	understanding the nature of the free state of riboswitch aptamers is important for illuminating common themes in gene regulation by riboswitches. prior evidence indicated the flavin mononucleotide (fmn)-binding riboswitch aptamer adopted a 'bound-like' structure in absence of fmn, suggesting only local conformational changes upon ligand binding. in the scope of pinpointing the general nature of such changes at the nucleotide level, we performed shape mapping experiments using the aptamer domain ...	2011	21745821
a novel organotypic dento-epithelial culture model: effect of fusobacterium nucleatum biofilm on b-defensin-2, -3, and ll-37 expression.	background: in the present study, the expression and localization of three epithelial peptides, human +¦-defensin (hbd)-2, -3, and cathelicidin (ll-37), were studied in an organotypic dento-epithelial (od-e) model exposed to fusobacterium nucleatum biofilm. methods: biofilm of f. nucleatum atcc 25586 or ahn 9508 were produced by culturing each strain on semipermeable membranes. the od-e model was constructed by seeding keratinocytes on fibroblast-containing collagen gels and by placing dentin pi ...	2011	21692631
invasive potential of gut mucosa-derived fusobacterium nucleatum positively correlates with ibd status of the host.	fusobacterium nucleatum is a heterogeneous oral pathogen that is also a common resident of the human gut mucosa. given that some strains of f. nucleatum are known to be invasive and proinflammatory in the oral mucosa, we compared strains isolated from patients with inflammatory bowel disease (ibd) with strains isolated from healthy controls to determine 1) whether this species was more commonly associated with ibd patients; and 2) whether gut-derived f. nucleatum strains from ibd patients showed ...	2011	21830275
efficacy of chlorhexidine digluconate-containing formulations and other mouthrinses against periodontopathogenic microorganisms.	objective: to determine in vitro the action of chlorhexidine digluconate and different commercially available mouthrinses on oral microorganisms. method and materials: minimal inhibitory concentrations and possible induction of resistance by chlorhexidine digluconate, an essential oil-containing mouthwash and an amine fluoride/stannous fluoride solution, were determined against microorganisms normally found in the oral cavity (10 streptococci, 2 enterobacteria, 1 candida albicans, 8 porphyromona ...	2011	21842009
[prevalence of helicobacter pylori detected by real-time pcr in the subgingival plaque of patients with chronic periodontitis].	helicobacter pylori plays a significant role in gastric disease. the aim of the present pilot study was to evaluate the prevalence of h. pylori detected by real-time polymerase chain reaction (real-time pcr) in the sub-gingival plaque of chronic periodontitis patients and to determine periodontopathogens profile of positive sites at h. pylori.	2011	21688602
spontaneously draining acute otitis media in children: an observational study of clinical findings, microbiology and clinical course.	abstract objectives: to study the outcome of acute otitis media (aom) with otorrhoea in children managed initially without antibiotics, in relation to bacterial and clinical findings, and to identify those who may benefit from antibiotics. methods: otherwise healthy, not otitis prone children aged 2-16 y, presenting with aom with spontaneous otorrhoea, were recruited from primary care and followed at selected ear, nose and throat (ent) clinics. specimens for bacterial investigations were obtaine ...	2011	21736512
Simultaneous detection of periodontal pathogens in subgingival plaque and placenta of women with hypertension in pregnancy.	BACKGROUND: There are many studies documenting increased prevalence of periodontal infection in women with preeclampsia. But, very few studies have attempted to establish causal relationship between the two. OBJECTIVE: To find out causal circumstantial evidence by isolating specific periodontal pathogens in oral and placental samples. MATERIALS AND METHODS: Antenatal periodontal screening and subgingival plaque collection was carried out in ten women with hypertension in pregnancy and ten normot ...	2011	21830010
Tongue piercing: the effect of material on microbiological findings.	Biofilms on oral piercings may serve as a bacterial reservoir and lead to systemic bacteremia or local transmission of pathogenic microbiota. The use of piercing materials which are less susceptible to biofilm accumulation could contribute to prevention of problems. The present study investigated whether there are microbiological differences in bacterial samples collected from tongue piercings made of different materials.	2011	21700161
adherence to streptococci facilitates fusobacterium nucleatum integration into an oral microbial community.	the development of multispecies oral microbial communities involves complex intra- and interspecies interactions at various levels. the ability to adhere to the resident bacteria or the biofilm matrix and overcome community resistance are among the key factors that determine whether a bacterium can integrate into a community. fusobacterium nucleatum is a prevalent gram-negative oral bacterial species that is able to adhere to a variety of oral microbes and has been implicated in playing an impor ...	2011	22202886
automated rna structure prediction uncovers a kink-turn linker in double glycine riboswitches.	the tertiary structures of functional rna molecules remain difficult to decipher. a new generation of automated rna structure prediction methods may help address these challenges but have not yet been experimentally validated. here we apply four prediction tools to a class of double glycine riboswitches that can bind two ligands cooperatively. a novel method (bppalign), rmdetect, jar3d, and rosetta 3d modeling give consistent predictions for a new stem p0 and a kink-turn motif. these elements st ...	2011	22192063
Antibacterial activity of sphingoid bases and fatty acids against gram-positive bacteria and gram-negative bacteria.	There is growing evidence that the role of lipids in innate immunity is more important than previously realized. How lipids interact with bacteria to achieve a level of protection, however, is still poorly understood. To begin to address mechanisms of antibacterial activity, we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of lipids common to the skin and oral cavity: sphingoid bases D-sphingosine, phytosphingosine, and dihydrosphingosine, and f ...	2011	22155833
antimicrobial efficacy of denture adhesives on some oral malodor-related microbes.	the objective of the study was to determine the antimicrobial efficacy of three denture adhesives toward streptococcus oralis, mutans, prevotella oralis and fusobacterium nucleatum. adhesives used were corega ultra(®), fixodent pro original(®) and biotene(®) denture grip. for streptococcus oralis and streptococcus mutans, four tubes of trypticase soy broth 10 ml and 1 g denture of adhesive were used. in addition four tubes of trypticase soy broth 10 ml without any denture adhesive was employed a ...	2011	22075753
a five-species transcriptome array for oral mixed-biofilm studies.	oral polymicrobial interactions and biofilm formation are associated with initiation and progression of caries, gingivitis, and periodontitis. transcriptome studies of such interactions, allowing a first mechanistic insight, are hampered by current single-species array designs.	2011	22194794
assessing the efficacy of vesicle fusion with planar membrane arrays using a mitochondrial porin as reporter.	reconstitution of functionally active membrane protein into artificially made lipid bilayers is a challenge that must be overcome to create a membrane-based biomimetic sensor and separation device. in this study we address the efficacy of proteoliposome fusion with planar membrane arrays. we establish a protein incorporation efficacy assay using the major non-specific porin of fusobacterium nucleatum (foma) as reporter. we use electrical conductance measurements and fluorescence microscopy to ch ...	2011	21295545
the effect of xylitol on the composition of the oral flora: a pilot study.	our aim was to investigate the effect of short-term xylitol consumption on the microbial composition of plaque and saliva.	2011	21311610
the origin of fusobacterium nucleatum involved in intra-amniotic infection and preterm birth.	objective. to evaluate the potential oral origin of fusobacterium nucleatum found in amniotic fluid of women at high risk of preterm birth. methods. a transversal study nested into a cohort study of women with preterm labor and/or preterm premature rupture of membranes was undergone. women with the presence of f. nucleatum in the amniotic fluid and their respective partners were invited to be examined for their periodontal health after delivery, and samples of saliva and subgingival plaque were ...	2011	21314291
structural basis of cooperative ligand binding by the glycine riboswitch.	the glycine riboswitch regulates gene expression through the cooperative recognition of its amino acid ligand by a tandem pair of aptamers. a 3.6 +à crystal structure of the tandem riboswitch from the glycine permease operon of fusobacterium nucleatum reveals the glycine binding sites and an extensive network of interactions, largely mediated by asymmetric a-minor contacts, that serve to communicate ligand binding status between the aptamers. these interactions provide a structural basis for how ...	2011	21439473
detection of periodontal bacteria in atheromatous plaques by nested polymerase chain reaction.	background. in recent years, increasing evidence regarding the potential association between periodontal diseases and cardiovascular diseases has been elicited. the available evidence underlines the importance of detecting periodontal pathogens on atheromatous plaques as the first step in demonstrating the causal relationship between both entities. it is the main aim of this investigation to detect periodontitis-associated bacteria from carotid artery atheromatous plaques recovered from patients ...	2011	21453047
the oral commensal, streptococcus gordonii, synergizes with tat protein to induce hiv-1 promoter activation in monocytes/macrophages.	trans-activator of transcription (tat) is an hiv-1 protein essential for viral replication. oral periodontopathogens (e.g. fusobacterium nucleatum) enhance hiv-1ltr promoter activation in monocytes/macrophages in absence of tat; however, some oral commensals fail to trigger this response. we sought to determine the effect of tat on hiv-1ltr promoter activation induced by the representative oral commensal streptococcus gordonii in monocytes/macrophages. s. gordonii enhanced hiv-1ltr reactivation ...	2011	21459369
clinical periodontal and microbiologic parameters in patients with rheumatoid arthritis.	background: a limited number of studies suggest the prevalence of periodontal pathogens in patients with rheumatoid arthritis (ra); however, results are inconsistent. the aim of this study was to investigate clinical periodontal and microbiologic parameters in patients with ra. methods: 66 patients with ra aged 49.5±8.4 years participated in the study. the periodontal situation was assessed with the periodontal screening index (psr(®)/psi); allocated to: healthy, gingivitis (psr(®)/psi score 0-2 ...	2011	21405936
clustering of subgingival microbial species in adolescents with periodontitis.	it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ...	2011	21410554
microbiologic results after non-surgical erbium-doped:yttrium, aluminum, and garnet laser or air-abrasive treatment of peri-implantitis: a randomized clinical trial.	background: the purpose of this study is to assess clinical and microbiologic effects of the non-surgical treatment of peri-implantitis lesions using either an erbium-doped:yttrium, aluminum, and garnet (er:yag) laser or an air-abrasive subgingival polishing method. methods: in a 6-month clinical trial, 42 patients with peri-implantitis were treated at one time with an er:yag laser or an air-abrasive device. routine clinical methods were used to monitor clinical conditions. baseline and 6-month ...	2011	21417591
novel model for multispecies biofilms that uses rigid gas-permeable lenses.	oral biofilms comprise complex multispecies consortia aided by specific inter- and intraspecies interactions occurring among commensals and pathogenic bacterial species. oral biofilms are primary initiating factors of periodontal disease, although complex multifactorial biological influences, including host cell responses, contribute to the individual outcome of the disease. to provide a system to study initial stages of interaction between oral biofilms and the host cells that contribute to the ...	2011	21421785
in-vivo-induced antigenic determinants of fusobacterium nucleatum subsp. nucleatum.	fusobacterium nucleatum plays a pivotal role in dental plaque biofilm formation and is known to be involved in chronic inflammatory systemic disease. however, limited knowledge of f. nucleatum genes expressed in vivo interferes with our understanding of pathogenesis. in this study, we identified f. nucleatum genes induced in vivo using in-vivo-induced antigen technology (iviat). among 30,000 recombinant clones screened, 87 reacted reproducibly with pooled sera from 10 patients with periodontitis ...	2011	21375706
rna-oligonucleotide quantification technique (roqt) for the enumeration of uncultivated bacterial species in subgingival biofilms.	approximately 35% of the species present in subgingival biofilms are as yet uncultivated, so their role in periodontal pathogenesis is unknown. the aim of the present study was to develop a high throughput method to quantify a wide range of cultivated and uncultivated taxa in subgingival biofilm samples associated with periodontal disease or health. oligonucleotides targeting the 16s ribosomal dna gene were designed, synthesized and labeled with digoxigenin. these probes were hybridized with the ...	2011	21375703
streptococcus cristatus attenuates fusobacterium nucleatum-induced cytokine expression by influencing pathways converging on nuclear factor-?b.	we previously reported that streptococcus cristatus, an oral commensal, was able to downregulate the interleukin-8 (il-8) response to fusobacterium nucleatum, a putative oral pathogen in oral epithelial cells. the aim of this study was to extend the understanding of how s. cristatus regulates cytokine expression in oral epithelial cells on a broad basis, and investigate whether the modulation of a toll-like receptor (tlr) pathway was involved in this process. kb and tert-2 cells were co-cultured ...	2011	21375705
association between periodontal pathogens and risk of nonfatal myocardial infarction.	the direct effect of periodontal pathogens on atherosclerotic plaque development has been suggested as a potential mechanism for the observed association between periodontal disease and coronary heart disease, but few studies have tested this theory.	2011	21375559
short- and medium-chain fatty acids exhibit antimicrobial activity for oral microorganisms.	this study assessed the antibacterial activity of short-, medium-, and long-chain fatty acids against various oral microorganisms.	2011	21333271
four cases of bacteraemia caused by fusobacterium nucleatum in febrile, neutropenic patients.	although bacteraemia caused by obligate anaerobic bacteria is a rare event, this phenomenon will be an emerging problem among oncohaematological patients. we report four cases of bacteraemia caused by fusobacterium nucleatum in febrile, neutropenic patients over a 10 month period. all patients had haematological malignancy and severe neutropenia, and three of them suffered from oral mucositis or oedema of the oral mucosal surfaces, which was the probable portal of entry. all isolated strains wer ...	2011	21415204
Microbiological analysis of gingivitis in pediatric patients under orthodontic treatment.	The aim of this study was to determine the relationship between gingival inflammation and changes in bacteria of the gingival sulcus in children in orthodontic treatment with brackets.	2011	22185242
fusobacterium nucleatum: an emerging gut pathogen?	the gram-negative, non-sporulating, obligately anaerobic species, fusobacterium nucleatum, is rapidly gaining notoriety as a pathogen with a surprising number of associated diseases. recently, we have found that f. nucleatum is a more common resident of the gi tract than originally thought, and thus, through several studies, we have attempted to determine its gut-relevant potential for virulence. we have found that f. nucleatum possesses a number of pathogenic traits with relevance to gut diseas ...	2011	22067936
Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis.	OBJECTIVE: The aim of this study was to determine the profiles of periodontopathogenic bacteria in a Chinese population using quantitative real-time polymerase chain reaction (qRT-PCR). MATERIALS AND METHODS: Twenty-four periodontally healthy Chinese subjects and 60 patients with chronic periodontitis (CP) were enrolled in this cross-sectional study. qRT-PCR was used to quantify Aggregatibacter actinomycetemcomit ...	2011	22169888
Isoflavonoids and Coumarins from Glycyrrhiza uralensis: Antibacterial Activity against Oral Pathogens and Conversion of Isoflavans into Isoflavan-Quinones during Purification.	Phytochemical investigation of a supercritical fluid extract of Glycyrrhiza uralensis has led to the isolation of 20 known isoflavonoids and coumarins, and glycycarpan (7), a new pterocarpan. The presence of two isoflavan-quinones, licoriquinone A (8) and licoriquinone B (9), in a fraction subjected to gel filtration on Sephadex LH-20 is due to suspected metal-catalyzed oxidative degradation of licoricidin (1) and licorisoflavan A (2). The major compounds in the extract, as well as 8, were evalu ...	2011	22074222
fusobacterium nucleatum adhesin fada binds vascular endothelial cadherin and alters endothelial integrity.	fusobacterium nucleatum is a gram-negative oral anaerobe, capable of systemic dissemination causing infections and abscesses, often in mixed-species, at different body sites. we have shown previously that f. nucleatum adheres to and invades host epithelial and endothelial cells via a novel fada adhesin. in this study, vascular endothelial (ve)-cadherin, a member of the cadherin family and a cell-cell junction molecule, was identified as the endothelial receptor for fada, required for f. nucleatu ...	2011	22040113
antibacterial effects of chemomechanical instrumentation and calcium hydroxide in primary teeth with pulp necrosis.	this study's purposes were to: evaluate the antibacterial effect of chemomechanical instrumentation and a calcium hydroxide-based dressing in primary teeth with pulp necrosis secondary to trauma; and detect the presence of fusobacterium nucleatum and black-pigmented rods in the canals of these teeth.	2011	21902996
The role of aggregation in Fusobacterium nucleatum- induced immune cell death.	Fusobacterium nucleatum, an anaerobic oral bacterium, has been shown to be highly abundant in endodontic infections. Its role in these infections remains unclear. Previous studies have shown that F. nucleatum could aggregate immune cells. We have demonstrated that F. nucleatum can induce significant apoptosis in peripheral blood mononuclear cells (PBMCs). In this in vitro study, we sought to determine what role this aggregation phenomenon has on the induction of apoptosis in PBMCs.	2011	22000457
Levels of periodontal pathogens in neonatal gastric aspirates and possible maternal sites of origin.	Maternal periodontal infection has been recognized as a risk factor for preterm and low birthweight infants. It is suspected that pathogens causing periodontal disease may translocate to the amniotic cavity and so contribute to triggering an adverse pregnancy outcome. This study aimed to determine levels and proportions of periodontal bacteria in neonatal gastric aspirates obtained from complicated pregnancies and the respective maternal oral and vaginal samples using a quantitative polymerase c ...	2011	21896155
Nonvolatile S-alk(en)ylthio-L-cysteine derivatives in fresh onion (Allium cepa L. cultivar).	The L-cysteine derivatives (R)-2-amino-3-(methyldisulfanyl)propanoic acid (S-methylthio-L-cysteine), (R)-2-amino-3-(propyldisulfanyl)propanoic acid (S-propylthio-L-cysteine), (R)-2-amino-3-(1-propenyldisulfanyl)propanoic acid (S-(1-propenylthio)-L-cysteine), and (R)-2-amino-3-(2-propenyldisulfanyl)propanoic acid (S-allylthio-L-cysteine) were prepared from 3-[(methoxycarbonyl)dithio]-L-alanine, obtained from the reaction of L-cysteine with methoxycarbonylsulfenyl chloride. The occurrence of these ...	2011	21854077
Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma.	An estimated 15% or more of the cancer burden worldwide is attributable to known infectious agents. We screened colorectal carcinoma and matched normal tissue specimens using RNA-seq followed by host sequence subtraction and found marked over-representation of Fusobacterium nucleatum sequences in tumors relative to control specimens. F. nucleatum is an invasive anaerobe that has been linked previously to periodontitis and appendicitis, but not to cancer. Fusobacteria are rare constituents of the ...	2011	22009989
fusobacterium nucleatum prosthetic hip infection in an adult with sickle cell-beta thalassemia.	fusobacterium nucleatum is an anaerobic gram-negative bacillus commensal to the human oropharynx and gastrointestinal tract which causes an array of human infection, yet it has never been associated with infection of prosthetic joints. we report the first case of prosthetic hip infection caused by f. nucleatum in a man with sickle cell-beta thalassemia.	2011	22002730
isolation and molecular identification of fusobacterium nucleatum from nigerian patients with oro-facial infections.	fusobacterium nucleatum is one of the most common anaerobic bacteria present in the oral cavity and is often isolated from infections involving other body sites.	2011	21984462
synergy in biofilm formation between fusobacterium nucleatum and prevotella species.	the formation of biofilm by anaerobic, gram-negative bacteria in the subgingival crevice plays an important role in the development of chronic periodontitis. the aim of this study was to characterize the role of coaggregation between fusobacterium nucleatum and prevotella species in biofilm formation. coaggregation between f. nucleatum and prevotella species was determined by visual assay. effect of co-culture of the species on biofilm formation was assessed by crystal violet staining. effect of ...	2011	21945879
analysis of symptomatic and asymptomatic primary root canal infections in adult norwegian patients.	this molecular study analyzed the microbiota of primary root canal infections from adult norwegian patients.	2011	21846535
extracellular deoxyribonuclease production by periodontal bacteria.	palmer lj, chapple ilc, wright hj, roberts a, cooper pr. extracellular deoxyribonuclease production by periodontal bacteria. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01451.x © 2011 john wiley & sons a/s background and objective: whilst certain bacteria have long been known to secrete extracellular deoxyribonuclease (dnase), the purpose in microbial physiology was unclear. recently, however, this enzyme has been demonstrated to confer enhanced virulence, enabling bacteria to evade the ...	2011	22150619
antimicrobial effects of o-cymen-5-ol and zinc, alone & in combination in simple solutions and toothpaste formulations.	this study aimed to evaluate antimicrobial effects of an o-cymen-5-ol/zinc system.	2011	21762153
[primary transport of acute patients. optimal use of rescue resources exemplified by the canton of graubünden].		2011	3927607
retreatodontics and ultrasonics.		2011	3162934