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inhibitory effects of funoran on the adherence and colonization of oral bacteria.funoran, a sulfated polysaccharide extracted from the seaweed gloiopeltis furcata, strongly inhibited the adsorption of mutans streptococci to saliva-coated hydroxyapatite (s-ha) used as an experimental pellicle and strongly desorbed cariogenic mutans streptococci pre-adsorbed to s-ha. colonization inhibition and anticariogenic effects of funoran were also investigated in experimental rats. the colonization of streptococcus cricetus e49 inoculated on the molar teeth of experimental rats administ ...19969151580
the influence of abutment surface roughness on plaque accumulation and peri-implant mucositis.bacterial adhesion to intra-oral, hard surfaces is firmly influenced by the surface roughness to these structures. previous studies showed a remarkable higher subgingival bacterial load on rough surfaces when compared to smooth sites. more recently, the additional effect of a further smoothening of intra-oral hard surfaces on clinical and microbiological parameters was examined in a short-term experiment. the results indicated that a reduction in surface roughness below r(a) = 0.2 microns, the s ...19969151584
the influence of periodontitis on the subgingival flora around implants in partially edentulous patients.the hypothesis that teeth act as reservoirs of micro-organisms for the colonization of oral implants has recently been stated several times. the present study aimed at examining, in partially edentulous patients with severe periodontitis, whether pockets around teeth and implants harbored a comparable micro-flora. in 6 patients (3 with refractory periodontitis and 3 with advanced chronic adult periodontitis), plaque samples were taken from a deep and shallow pocket around both teeth and implants ...19969151609
microbiology of chronic maxillary sinusitis: comparison between specimens obtained by sinus endoscopy and by surgical drainage.the aerobic and anaerobic microbiology of sinus aspirates obtained during surgery was compared with culture of samples obtained by endoscopy. six patients with chronic maxillary sinusitis were evaluated. polymicrobial flora was found in all specimens (two-to-five isolates/sample). a total of 24 isolates (18 anaerobic, five aerobic and one micro-aerophilic) was obtained from sinus aspirates, and 25 isolates (16 anaerobic and nine aerobic) were found in endoscopic specimens. the predominant organi ...19979152041
possible lethal enhancement of toxins from putative periodontopathogens by nicotine: implications for periodontal disease.to test the hypothesis that lethal synergy in the chick embryo model may occur between nicotine and bacterial products (cell-free extracellular toxins and cell lysates) of five putative periodontopathogens.19979155677
characterization of bacteroides forsythus isolates.fifteen bacteroides forsythus strains freshly isolated from patients with periodontitis were used together with three collection strains and one type strain for characterization of growth on various media; determination of enzymatic profiles, antibiotic susceptibility profiles, 16s rrna ribotypes, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) outer membrane protein profiles, and pathogenicity; and gas chromatography analysis by using a wound chamber model in rabbits. all s ...19979163447
rheumatoid factor from periodontitis patients cross-reacts with epitopes on oral bacteria.the objective of this study was to determine the antigenic specificity of rheumatoid factor (rf) that had previously been reported in the serum of patients with periodontitis.19969171508
protein degradation by prevotella intermedia and actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum.the proteolytic activities of oral bacteria are thought to play an important role in the aetiology of dental abscesses. bacteria-derived proteases may contribute to tissue destruction, and are likely to impair host defence by degrading immunoglobulins and complement. degraded periodontal tissue and tissue fluid are likely to constitute essential sources of nutrients in the abscess. tissue fluid, which is derived from serum, is rich in protein and poor in carbohydrate, suggesting that breakdown o ...19979178115
orogenital contact: a cause of chorioamnionitis?fusobacterium nucleatum and capnocytophaga species are common oral pathogens and infrequent causes of systemic infection in patients with compromised immunity or disrupted mucosal integrity. the isolation of both organisms from a clinical specimen suggests an oral source of infection.19949205437
associations of endodontic symptoms and signs with particular combinations of specific bacteria.significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ...19969206427
an in vitro study of the coronal leakage of two root canal sealers using an obligate anaerobe microbial marker.the aim of this in vitro study was to investigate the coronal leakage of obligate anaerobes into root canals obturated with lateral condensation of cold gutta-percha with two root canal sealers. sixty extracted human teeth with straight, single root canals were prepared using the modified double-flared technique with balanced force under copious irrigation until the master apical file was size 40. the teeth were divided randomly into experimental groups (40 teeth) and control groups (20 teeth). ...19969206441
in vitro activity of bay 12-8039, a new 8-methoxyquinolone, compared to the activities of 11 other oral antimicrobial agents against 390 aerobic and anaerobic bacteria isolated from human and animal bite wound skin and soft tissue infections in humans.the in vitro activity of bay 12-8039, a new oral 8-methoxyquinolone, was compared to the activities of 11 other oral antimicrobial agents (ciprofloxacin, levofloxacin, ofloxacin, sparfloxacin, azithromycin, clarithromycin, amoxicillin clavulanate, penicillin, cefuroxime, cefpodoxime, and doxycycline) against 250 aerobic and 140 anaerobic bacteria recently isolated from animal and human bite wound infections. bay 12-8039 was active against all aerobic isolates, both gram-positive and gram-negativ ...19979210683
uvulitis caused by anaerobic bacteria.i present two children with bacteremic uvulitis due to anaerobic bacteria.19979220512
disinfection by calcium hydroxide pastes of dentinal tubules infected with two obligate and one facultative anaerobic bacteria.bovine dentine cylinders were experimentally infected with actinomyces israelii, fusobacterium nucleatum, or enterococcus faecalis. the latter is a facultative anaerobic bacteria and the others are obligate anaerobes commonly found in endodontic infections. the infected specimens were exposed to pastes of calcium hydroxide mixed with saline solution or camphorated paramonochlorophenol for periods of 1 h, 1 day, and 1 week. the viability of bacteria after these exposure times was evaluated by spe ...19969220753
immune response to fusobacterium nucleatum, prevotella intermedia and other anaerobes in children with acute tonsillitis.the number of aerobic and anaerobic bacteria was determined in the saliva of 20 children with acute group a beta-haemolytic streptococcal (gabhs) pharyngo-tonsillitis, and 20 with acute non-gabhs tonsillitis. antibody titres to four gram-negative anaerobic bacilli that reside in the oropharynx (fusobacterium nucleatum, prevotella intermedia, porphyromonas gingivalis, and actinobacillus actinomycetemcomitans) were determined in these and 20 control patients. an average of 8.8 aerobic and anaerobi ...19979222046
occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: microbiological findings.bacterial invasion in roots of periodontally diseased teeth, which has been recently documented using cultural and microscopic techniques, may be important in the pathogenesis of periodontal disease. the purpose of this investigation was to determine the occurrence and the species of invading bacteria in radicular dentin of periodontally diseased teeth. samples were taken from the middle layer of radicular dentin of 26 periodontally diseased teeth. 14 healthy teeth were used as controls. dentin ...19979226388
multiple c-terminal serine phosphorylation accompanies both protein kinase c-dependent and -independent activation of cytosolic 85 kda phospholipase a2 in macrophages.exposure of mouse macrophages to either phorbol ester or certain bacteria was previously shown to cause increased phosphorylation of the cytosolic 85 kda phospholipase a2 as well as a stable increase in its catalytic activity. we have now attempted to map the major phosphorylation sites on the enzyme in such cells. phosphorylation occurred on serine residues without a detectable increase in either phosphothreonine or phosphotyrosine. after cnbr cleavage five fragments showed increased 32p labell ...19979230120
the relationship between gingivitis and the serum antibodies to the microbiota associated with periodontal disease in children with down's syndrome.gingival inflammation in down's syndrome children (ds) develops earlier and is more rapid and extensive than in non-ds children. abnormalities in host response to the oral flora have been proposed as etiological factors of this gingival inflammation. however, the relationship between gingivitis and the host response to oral microorganisms in ds by age has not been determined. the objective of this study was to clarify this relationship. sera were obtained from 75 ds subjects (aged 2 to 18 years) ...19979249633
host modulation of tissue destruction caused by periodontopathogens: effects on a mixed microbial infection composed of porphyromonas gingivalis and fusobacterium nucleatum.these studies determined the ability of selected periodontopathogens to synergistically initiate soft tissue destruction in a murine abscess model. the development of immunity following recovery from infection or by active immunization was also examined. mice were infected with p. gingivalis w50, f. nucleatum t18, or a combination of the two microorganisms. f. nucleatum caused only a localized lesion in contrast to p. gingivalis which caused a spreading suppurative inflammatory lesion of the ski ...19979250777
beta-lactamase producing bacteria in adult periodontitis.in 23 untreated adult periodontitis patients, the occurrence of beta-lactamase producing periodontal bacteria was determined. in addition to non-selective isolation media, selective isolation and growth of beta-lactamase positive subgingival bacterial species was carried out on blood agar plates supplemented with amoxicillin and plates with amoxicillin+clavulanic acid. porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, peptostreptococcus micros, fusobacterium ...19979266340
increased susceptibility of rag-2 scid mice to dissemination of endodontic infections.specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. to investigate this question we compared periapical-lesion pathogenesis in rag-2 severe combined immunodeficient (scid) mice with immunocompetent control mice following surgical pulp exposure. in order to equalize the bacterial challenge, an infection protocol ...19979284152
enzymatic removal and disinfection of bacterial biofilms.model biofilms of staphylococcus aureus, staphylococcus epidermidis, pseudomonas fluorescens, and pseudomonas aeruginosa were made on steel and polypropylene substrata. plaque-resembling biofilms of streptococcus mutans, actinomyces viscosus, and fusobacterium nucleatum were made on saliva-coated hydroxyapatite. the activity of enzymes against bacterial cells in biofilm was measured by fluorescence microscopy and an indirect conductance test in which evolution of carbon dioxide was measured. glu ...19979293025
recently described clinically important anaerobic bacteria: taxonomic aspects and update.a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ...19979310640
dental implant infections.dental implants provide a restorative tool to support crowns, bridge abutments, and removable dentures. osseointegrated implants are titanium posts that are surgically implanted in alveolar bone. a tight immobile bond (osseointegration) forms between bone and titanium, and prosthetic and restorative fixtures are attached to the implants. titanium implants differ from natural teeth, which may make them more susceptible to mechanical stress. a small proportion of implants are not successful and ma ...19979310682
pore-forming molecules in gram-negative anaerobic bacteria.little information is available about porin molecules in anaerobes. porins from bacteroides fragilis and porphyromonas, fusobacterium, and campylobacter species have been described. a pore-forming outer membrane (om) porin protein was isolated from b. fragilis (omp-200); it is exposed at the cell surface and dissociated by boiling and application of reducing agents. fusobacterium nucleatum foma, an om porin protein of 40 kd, had a deduced topology of foma similar to that of established porins, d ...19979310708
cross-infection from periodontitis sites to failing implant sites in the same mouth.twenty-five tooth and implant sites in nine patients were investigated for the presence of putative periodontopathic organisms, using specific dna probes for actinobacillus actinomycetemcomitans, porphromonas gingivalis, prevotella intermedia, eikenella corrodens, fusobacterium nucleatum, treponema denticola, and campylobacter recta. five of nine patients showed a likelihood of transmission from tooth to implant sites. these patients also showed a high number of putative periodontopathic organis ...19979337029
putative periodontal pathogens in subgingival plaque of young adults with and without early-onset periodontitis.this study assessed the associations between putative periodontal pathogens and early-onset periodontitis (eop) in a population of 248 subjects, 13 to 19 years of age at baseline, derived from a representative sample of u.s. young adults. the subjects were selected based on the presence or absence of attachment loss at baseline. the attachment level was assessed clinically at baseline and at a 6-year follow-up examination, and the presence of 7 bacterial species was assessed at follow-up using d ...19979358364
coronal leakage of mixed anaerobic bacteria after obturation and post space preparation.the purpose of this study was to assess bacterial leakage of a mixed anaerobic community of organisms in obturated canals after post space preparation.19979377197
experimental gingivitis in periodontitis-susceptible subjects.the purpose of this study was to evaluate clinical, microbiological, and gingival crevicular fluid (gcf) profiles in periodontitis-resistant and periodontitis-susceptible subjects during 4 weeks of experimental gingivitis. experimental groups of similar ages were defined as gingivitis controls (gc; n = 10) and history of rapidly progressive periodontitis (rpp; n = 10), respectively. prior to baseline, all subjects achieved good plaque control (plaque index (p1i) approximately 0) and gingival hea ...19979378832
identification of a fusobacterium nucleatum pk1594 galactose-binding adhesin which mediates coaggregation with periopathogenic bacteria and hemagglutination.attachment of fusobacterium nucleatum to various oral surfaces is mediated by several adhesins anchored on its outer surface. monoclonal antibodies (mabs) were prepared and used to identify the putative galactose-binding adhesin of f. nucleatum pk1594. four unique mabs, 8g7, 26b9, 28g11, and 29d4, were isolated on the basis of their ability to inhibit coaggregation of f. nucleatum pk1594 with porphyromonas gingivalis pk1924. all four mabs were also capable of inhibiting galactose-inhibitable int ...19979393820
"checkerboard" versus culture: a comparison between two methods for identification of subgingival microbiota.the present study compared the "checkerboard" dna-dna hybridization methodology with culture techniques for the analysis of the composition of the subgingival microbiota. 70 subjects, presenting with a variety of periodontal conditions, contributed with a total of 283 subgingival plaque samples analyzed with respect to the following species: porphyromonas gingivalis, prevotella intermedia/prevotella nigrescens, fusobacterium nucleatum, campylobacter rectus, eikenella corrodens, bacteroides forsy ...19979395099
reduced serum igg reactivities with bacteria from dental plaque in hiv-infected persons with periodontitis.serum samples were obtained from 44 hiv-seropositive (hiv+) and 37 hiv-seronegative (hiv-) persons that were grouped according to periodontal status. serum igg and iga reactivities towards streptococcus mutans, actinobacillus actinomycetemcomitans, porphyromonas gingivalis. prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum were measured by means of elisa. hiv+ persons with chronic marginal periodontitis showed significantly lower igg reactivities to the periodontal pathoge ...19979402504
comparison of the subgingival microbiota of periodontally healthy and diseased adults in northern cameroon.our study is the 1st report on subgingival microbiota in adult cameronians. the aim was to investigate, using the checkerboard dna-dna hybridization technique, the prevalence of 18 oral species in subgingival plaque samples obtained from sex- and age-matched cameronian adults with and without periodontal destruction. we also compared cultivation and the affirm dp test with the checkerboard technique in their capability to detect some selected species among the 18. 21 adult periodontitis patients ...19979402505
[microflora of periodontal pockets in advanced periodontitis].the aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. from each subject 16-20 samples were taken using paper points. pooled sample after 60 s. mixing was serially diluted in reduced bhi. for total cell counts and for the isolation of black pigmented anaerobes brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without kanamycin-vancomycin mixture and bm agar plates were used. for isolation of a. actinomycetemcomitans ...19979411079
some properties of gamma-glutamyl transpeptidase from fusobacterium nucleatum.a series of chromatographic techniques was used to purify gamma-glutamyl transpeptidase from fusobacterium nucleatum atcc 23726. the purified enzyme was homogenous with a molecular weight (mw) of approximately 101 kd consisting of two different subunits with mw of 67 kd and 31 kd. its distribution by treatment of lysozyme-edta suggested that the enzyme was a periplasmic protein. the pl of the enzyme was 5.9 to 6.2, and the optimum ph of the transpeptidation and the hydrolytic reaction was 8.0. g ...19979418037
subgingival temperature: relation to gingival crevicular fluid enzymes, cytokines, and subgingival plaque micro-organisms.there have been no reports on the relationship of subgingival temperature to specific gingival crevicular fluid (gcf) components. therefore, the purpose of this cross-sectional study was to determine whether there was any relationship between subgingival temperature and gcf levels of neutrophil elastase (ne), myeloperoxidase (mpo), beta-glucuronidase (bg), interleukin-1 alpha (il-1), and interferon alpha (ifn). furthermore, another objective was to confirm an association of subgingival temperatu ...19979442427
evaluation of root canal bacteria and their antimicrobial susceptibility in teeth with necrotic pulp.this study aimed to evaluate the microbiota of necrotic pulp in teeth without carious lesions where the crown and root were intact and to test the sensitivity of this microbiota to antibiotics in order to improve treatment. the necrotic pulp was sampled from 26 single-rooted teeth in intact pulp chambers. a total of 84 strains were isolated. the number of species isolated per tooth varied from 2 to 8, with a strong component (81%) of anaerobic bacteria. the most commonly represented species were ...19979467386
evidence for serum immunoglobulin g (igg) antibody responses in macaca fascicularis identified by monoclonal antibodies to human igg subclasses.this investigation determined the capacity of murine monoclonal antibodies directed to human immunoglobulin g (igg) subclasses to identify molecules with conserved epitopes in the serum of the nonhuman primate, macaca fascicularis. we subsequently utilized this cross-reactivity to document the characteristics of igg subclass antibody responses in m. fascicularis to parenteral immunization with intact oral microorganisms, antigens from oral microorganisms, and finally a defined protein toxin, tet ...19979467387
characteristics of systemic antibody responses of nonhuman primates to cell envelope and cell wall antigens from periodontal pathogens.the immune response of the primate, macaca fascicularis, to cell envelope (cea) or cell wall (cwa) antigens of several periodontal pathogens was examined to develop a strategy to interfere with ligature-induced periodontitis. animals were parenterally immunized with cea of either porphyromonas gingivalis, prevotella intermedia or a combination of cea/cwa of campylobacter rectus, fusobacterium nucleatum and actinomyces viscosus. serum samples were taken every 2-4 weeks over a 4-month period, whic ...19979467388
arbitrarily primed polymerase chain reaction fingerprinting and clonal analysis of oral fusobacterium nucleatum isolates.f. nucleatum is the most commonly isolated microorganism from subgingival plaque, but the role of this microorganism in periodontal diseases remains undefined. arbitrarily primed polymerase chain reaction (ap-pcr) was evaluated as a method for fingerprinting f. nucleatum isolates and for use in clonal analysis. pulsed field gel electrophoresis was used to further differentiate f. nucleatum isolates, with identical ap-pcr patterns. extremely heterogeneous ap-pcr fingerprints were observed among t ...19979467390
coaggregation of candida albicans with oral fusobacterium species.nine strains of oral fusobacterium were examined for their ability to coaggregate in vitro with four strains of the oral yeast. candida albicans. all of the fusobacterium nucleatum strains and fusobacterium periodontium and fusobacterium sulci coaggregated to various degrees with all of the candida strains. fusobacterium alocis, fusobacterium mortiferum and fusobactrium simiae strains did not coaggregate with any of the candida strains. exposure of the coaggregating fusobacterium strains but not ...19979467403
salivary iga responses to bacteria in dental plaque as related to periodontal and hiv infection status.levels of total iga and specific iga reactive with streptococcus mutans, actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum were measured by elisa in parotid saliva from hiv+ and hiv- persons with healthy gingiva (hg), chronic gingivitis, chronic marginal periodontitis (cmp), or necrotizing ulcerative periodontitis (nup). when the hiv+ group was compared with the hiv- group regardless of periodontal status, tot ...19979469606
an evaluation of microbial coronal leakage in the restored pulp chamber of root-canal treated multirooted teeth.this in vitro study investigated the effect of a resin-reinforced glass ionomer lining material on the coronal leakage of a mixed obligate microbial marker in maxillary molars obturated with lateral condensation of cold gutta-percha and tubliseal sealer, after 2 years' storage. forty maxillary first molars were prepared chemomechanically to a size 30-40 master apical file. the teeth were divided into an experimental group (30 teeth) and control group (10 teeth). in the experimental group, the fl ...19979477821
[pulmonary infection caused by fusobacterium nucleatum].thoracic infection by anaerobes are uncommon diseases and often presents difficulty in diagnosis. we report a case of thoracic infection due to fusobacterium nucleatum. a 60-year old man presented a lesion infiltrating from the lung to the thoracic wall. fusobacterium nucleatum was isolated on pus collected. treatment by penicillin, metronidazole combined with surgical drainage was highly effective.19979480485
in vitro determination of direct antimicrobial effect of calcium hydroxide.the objective of this study was to determine in vitro the time required for calcium hydroxide in direct contact with microorganisms to express its antimicrobial effect. the microorganisms used were: micrococcus luteus (atcc-9341), staphylococcus aureus (atcc-6538), fusobacterium nucleatum (atcc-25586), pseudomonas aeruginosa (atcc-27853), escherichia coli, and streptococcus sp. the strains were cultivated in brain heart infusion (bhi), with the exception of f. nucleatum (bhi-pras). pure and mixe ...19989487859
microbial complexes in subgingival plaque.it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ...19989495612
subgingival distribution of periodontopathic bacteria in adult periodontitis and their susceptibility to minocycline-hcl.the purpose of this study was to investigate the distribution of several periodontopathic bacteria in adult periodontitis, their in vitro susceptibility to minocycline-hcl, and whether the efficacy of the drug changes with a decrease in bacterial susceptibility. twenty-one patients (43 to 75 years old) with 62 periodontal lesions from pockets > or =4 mm participated in the study. after subgingival sampling, an ointment containing 2% minocycline-hcl was applied locally to the selected pockets onc ...19989527568
killing of fusobacterium nucleatum, porphyromonas gingivalis and prevotella intermedia by protegrins.protegrins are broad spectrum antibiotic peptides isolated from porcine leukocytes. in this study, we (i) examine the sensitivity of gram-negative, anaerobic periodontal pathogens to synthetic protegrins; (ii) determine the relative potencies of protegrin congeners against these bacteria; and (iii) compare the potency of protegrins with other antibiotic peptides, including magainin msi-78, tachyplesin i, cecropin p1, human defensins hnp-1-3, and clavanin a. synthetic l- and d-enantiomers of prot ...19989553868
perforation repair comparing mineral trioxide aggregate and amalgam using an anaerobic bacterial leakage model.the purpose of this study was to evaluate the ability of mineral trioxide aggregate (mta) and amalgam to seal furcal perforations in extracted human molars using an anaerobic bacterial leakage model. furcal perforations were made in 39 maxillary and mandibular human molars with a high-speed bur. these were randomly divided into two experimental groups of 18, with the remaining three teeth used as positive controls. experimental group 1 was repaired with mta and group 2 with amalgam. three additi ...19989558584
beta-sheet antibiotic peptides as potential dental therapeutics.small, cysteine-rich, beta-sheet peptide antibiotics are found throughout the animalia. though broad spectrum in potential, they may exert selective antimicrobial effects under certain conditions. we have explored the antimicrobial properties of two families of beta-sheet peptide antibiotics, defensins and protegrins, against periodontopathic bacteria. the rabbit defensin np-1 was active against facultative gram-negative bacteria associated with early onset periodontitis, including actinobacillu ...19989573496
effect of red blood cells on the growth of porphyromonas endodontalis and microbial community development.establishment of a microbial community in the root canal system depends on numerous factors, of which nutrient availability may be one of the most important. we hypothesized that the presence of red blood cells or hemoglobin in this environment could cause shifts in microbial composition of communities, resulting in organisms such as porphyromonas endodontalis becoming more dominant. an in vitro model system using mixed, batch cultures was performed with the bacteria p. endodontalis, fusobacteri ...19989573801
acid tolerance and acid-neutralizing activity of porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum.the tolerance to acid and the acid-neutralizing activity of three important periodontopathic bacteria, porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum were studied. p. gingivalis strains grew only at neutral ph and did not utilize glucose, whereas strains of p. intermedia and f. nucleatum could grow under acidic conditions and increased their growth by utilizing glucose. p. gingivalis tended to raise the culture ph during growth. p. intermedia and f. nucleatum raised ...19979573805
characterization of a novel n-acetylneuraminic acid-specific fusobacterium nucleatum pk1594 adhesin.fusobacterium nucleatum has been identified as significantly associated with sites with active periodontal disease and, as a group, the oral fusobacteria coaggregate with members of all oral bacteria genera tested. monoclonal antibodies were prepared and used in conjunction with other potential inhibitors, such as simple sugars and amino acids, to characterize coaggregation interactions, of f. nucleatum pk1594. four unique monoclonal antibodies, 5h11, 14c7, 19f2 and 29c12, were obtained by their ...19989573822
helicobacter pylori adheres selectively to fusobacterium spp.helicobacter pylori strains atcc 43504 and atcc 43629 were tested for their ability to coaggregate with 79 strains of bacteria representing 16 genera. all except two of the strains were of human origin, and most of the strains were isolated from the oral cavity. the helicobacters failed to coaggregate with all strains except the fusobacteria. several coaggregations were partially or completely inhibited by lactose. strong coaggregation was seen with each of four subspecies of fusobacterium nucle ...19989573823
trovafloxacin compared with levofloxacin, ofloxacin, ciprofloxacin, azithromycin and clarithromycin against unusual aerobic and anaerobic human and animal bite-wound pathogens.the activity of trovafloxacin and five other oral agents against 250 aerobic and 137 anaerobic strains isolated from human and animal bite wounds was determined by an agar dilution method. trovafloxacin was active against all aerobic and fastidious facultative isolates at < or = 0.5 mg/l and all anaerobes at < or = 2 mg/l (bacteroides tectum, porphyromonas salivosa and prevotella heparinolytica, < or = 0.25 mg/l; porphyromonas spp., < or = 0.5 mg/l; prevotella spp. and peptostreptococci, < or = ...19989578167
the use of a 16s rdna directed pcr for the detection of endodontopathogenic bacteria.the study evaluates a 16s rdna directed polymerase chain reaction (pcr) to detect and differentiate bacteria in necrotic root canal samples. the examination focused on species that are fastidious concerning culture or are difficult to differentiate after culturing by biochemical methods. in the described pcr assay, a universal 16s rdna directed forward primer in combination with a highly specific reversed one was used to amplify taxon specific gene fragments of 230 to 950 bp length. a similar pc ...19979587296
in-vitro antibacterial effects of extracts of nigerian tooth-cleaning sticks on periodontopathic bacteria.aqueous extracts from 8 plants used for tooth-cleaning in nigeria were tested for their ability to inhibit the growth of five periodontopathic bacteria, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, eikenella corrodens and campylobacter rectus. extracts of all the plants except that of massularia acuminata exhibited varying growth inhibitory potentials on the microorganisms. extract of terminalia glaucescens showed the widest spectrum of activity, inhibiting the growt ...19959590895
activities of hmr 3004 (ru 64004) and hmr 3647 (ru 66647) compared to those of erythromycin, azithromycin, clarithromycin, roxithromycin, and eight other antimicrobial agents against unusual aerobic and anaerobic human and animal bite pathogens isolated from skin and soft tissue infections in humans.the activities of hmr 3004 and hmr 3647 and comparator agents, especially macrolides, were determined by the agar dilution method against 262 aerobic and 120 anaerobic strains isolated from skin and soft tissue infections associated with human and animal bite wounds. hmr 3004 and hmr 3647 were active against almost all aerobic and fastidious facultative isolates (mic at which 90% of the isolates are inhibited [mic90], < or = 0.5 and 1 microg/ml, respectively) and against all anaerobes [bacteroid ...19989593139
volatile fatty acid, metabolic by-product of periodontopathic bacteria, induces apoptosis in wehi 231 and raji b lymphoma cells and splenic b cells.the ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine wehi 231 cells, splenic b cells, and human raji cells was examined. the culture filtrate of porphyromonas gingivalis, prevotella loescheii, and fusobacterium nucleatum, which contains high a percentage of butyric acid, induced dna fragmentation in wehi 231 cells. volatile fatty acid, especially butyric acid, significantly suppressed b-cell viability in a concentration-dependent ...19989596720
granulocyte-macrophage colony-stimulating factor amplification of interleukin-1beta and tumor necrosis factor alpha production in thp-1 human monocytic cells stimulated with lipopolysaccharide of oral microorganisms.cytokines, including granulocyte-macrophage colony-stimulating factor (gm-csf), are used to assist in bone marrow recovery during cancer chemotherapy. interleukin-1beta (il-1beta) and tumor necrosis factor alpha (tnf-alpha) play important roles in inflammatory processes, including exacerbation of periodontal diseases, one of the most common complications in patients who undergo this therapy. a human monocyte cell line (thp-1) was utilized to investigate il-1beta and tnf-alpha production followin ...19989605989
studies on fusobacteria associated with periodontal diseases.the physiological and metabolic characteristics of representative isolates of the various subspecies of fusobacterium nucleatum were investigated by growing them in continuous culture in chemically-defined, media. behaving almost identically, these organisms were found to obtain energy from the fermentation of simple carbohydrates such as glucose or fructose or from the fermentation of certain amino acids, free or in the form of small peptides. the latter can be attacked by aminopeptidase activi ...19989612984
development of an anaerobic bacterial leakage model.the majority of bacteria associated with infections of endodontic origin are strict anaerobes. the purpose of this study was to develop an endodontic microleakage model using strict anaerobic bacteria in a two-chamber system. nine species of anaerobic bacteria were tested for viability and detection by either turbidity or color change of the broth. a survey of ph chromogenic substrates revealed that bromcresol purple (ph 5.2 = yellow, ph 6.8 = purple) could be used as a chromogenic indicator to ...19989641124
the effect of herbal extracts in an experimental mouthrinse on established plaque and gingivitis.the purpose of the present study was to establish in vitro the inhibiting effect of a herbal extract mixture on a selected number of micro-organisms and to test in vivo the effect of a mouthwash containing 6.3 mg/ml herbal extract mixture on plaque and gingivitis as compared to a minus active control mouthrinse. the herbal extract was a mixture of: juniperus communis (juniper), urtica dioca (nettle), achillaea millefolium (yarrow); 1:1:1. in the study, in-vitro, the effect of pure herbal extract ...19989650877
subgingival microflora associated with nifedipine-induced gingival overgrowth.the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ...19989660335
severity of localized juvenile periodontitis as related to polymorphonuclear chemotaxis and specific microbial isolates.the purpose of this study was twofold: (a) to determine if an association exists between severity of localized juvenile periodontitis (ljp) and impairment of polymorphonuclear leukocyte (pmn) chemotaxis and/or colonization by specific microbial isolates; (b) to determine if the number of specific microbial isolates, i.e., actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, eikenella corrodens, and campylobacter rectus correlates to clin ...19989663117
the bacteria of periodontal diseases. 19949673163
[spinal epidural abscess due to fusobacterium nucleatum].we report a case of spinal epidural abscess. in our knowledge this is the first ever reported case due to fusobacterium nucleatum. an 83 year old female patient with a history of lumbar trauma presented severe lumbalgia. magnetic resonance showed the unusual anterior location, complicated by psoas abscess. laminectomy and lumbar drainage were performed. she was treated with ceftriaxone plus metronidazole during 10 days. the evolution was favourable without any residual neurological morbidity.19979674195
dentilisin activity affects the organization of the outer sheath of treponema denticola.prolyl-phenylalanine-specific serine protease (dentilisin) is a major extracellular protease produced by treponema denticola. the gene, prtp, coding for the protease was recently cloned and sequenced (k. ishihara, t. miura, h. k. kuramitsu, and k. okuda, infect. immun. 64:5178-5186, 1996). in order to determine the role of this protease in the physiology and virulence of t. denticola, a dentilisin-deficient mutant, k1, was constructed following electroporation with a prtp-inactivated dna fragmen ...19989683480
clonal analysis by ribotyping of fusobacterium nucleatum isolates obtained from healthy young adults with optimal plaque control.fusobacterium nucleatum is a gram-negative anaerobic rod implicated in the pathogenesis of periodontal disease. however, this organism has also been frequently identified in high numbers in healthy adults. these observations suggest that the species may comprise different clonal types, some of which may participate in disease. the purpose of the present investigation was to use restriction endonuclease analysis (rea) and ribotyping to characterize f. nucleatum clonal types isolated from healthy ...19989689613
experimental root canal infections in conventional and germ-free mice.a small animal model was evaluated to study the interrelationships between microorganisms after their implantation in root canals (inferior central incisors) using germ-free (gf) and conventional (cv) mice. the selected microorganisms were: porphyromonas endodontalis (atcc 35406), eubacterium lentum (atcc 25559), peptostreptococcus anaerobius (atcc 27337), fusobacterium nucleatum (atcc 10953), escherichia coli (atcc 25922), and enterococcus faecalis (atcc 4083). only p. anaerobius, e. coli, and ...19989693583
an aminopeptidase nutritionally important to fusobacterium nucleatum.the properties of an aminopeptidase (ap) from fusobacterium nucleatum were studied in view of the fact that this organism, along with other gram-negative anaerobes involved in periodontal diseases, survives in the subgingival environment by obtaining energy via the fermentation of a small number of peptide-derived amino acids. the ap was found to be cell-associated and was isolated from disrupted chemostat-grown cells. it was purified by (nh4)2so4 fractionation, two column chromatographic steps ...19989695913
bone resorption caused by three periodontal pathogens in vivo in mice is mediated in part by prostaglandin.gingival inflammation, bacterial infection, alveolar bone destruction, and subsequent tooth loss are characteristic features of periodontal disease, but the precise mechanisms of bone loss are poorly understood. most animal models of the disease require injury to gingival tissues or teeth, and the effects of microorganisms are thus complicated by host responses to tissue destruction. to determine whether three putative periodontal pathogens, porphyromonas gingivalis, campylobacter rectus, and fu ...19989712762
expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue.human beta-defensin-1 (hbd-1) is a member of the family of small cationic antimicrobial peptides that have been identified in several mucosal epithelia. because human gingival epithelium is a site that is constantly challenged by oral microorganisms, we examined the expression of hbd-1 in human gingival epithelial and fibroblast cell cultures and tissue samples. cell cultures were challenged with cell wall extracts of porphyromonas gingivalis or fusobacterium nucleatum, escherichia coli lipopoly ...19989712771
in vitro activity of hmr 3647 against anaerobic bacteria.the aim of the present investigation was to determine the in vitro activity of hmr 3647 compared with other antimicrobial agents against anaerobic bacteria. the activity of hmr 3647 was determined against 342 clinical isolates of anaerobic bacteria by the agar dilution method and was compared with azithromycin, clarithromycin, roxithromycin, erythromycin, cefoxitin, imipenem, clindamycin and metronidazole. among the macrolides hmr 3647 and among the beta-lactams imipenem were the most active age ...19989720465
superficial suppurative thrombophlebitis in children, caused by anaerobic bacteria.the purpose of this study was to present the microbiology and clinical features of six children with superficial suppurative thrombophlebitis (sst) caused by anaerobic bacteria.19989722004
subgingival microbial profile of papillon-lefèvre patients assessed by dna-probes.the prevalence of 18 selected bacterial species was assessed by means of "checkerboard" dna-dna hybridisation in a group of 12 saudi-arabian adolescents with papillon-lefèvre syndrome. a total of 36 tooth sites were investigated. the patients exhibited severe periodontal disease with deep pockets. all 12 patients harboured the putative bacterial pathogens p. intermedia, f. nucleatum, p. micros and s. intermedius while t. denticola, b. forsythus, p. nigrescens, e. corrodens, s. noxia and c. rectu ...19989722266
oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis.the present study primarily aimed at investigating the oral microbiota in smokers and non-smokers with established gingivitis and monitoring its composition during experimental gingivitis. secondly, it aimed at examining whether the composition of the microbiota is associated with different levels of gingival inflammation during this experimental gingivitis trial. for this purpose, 25 non-dental university students with gingivitis were recruited. 11 subjects were smokers and 14 were non-smokers. ...19989722273
preterm birth: associations with genital and possibly oral microflora.opportunistic pathogenic microbes are indigenous to the female lower genital tract and etiologic in many types of pelvic infections and, apparently, a portion of preterm birth (ptb) cases. bacterial vaginosis (bv) is a clinical syndrome based on an altered genital microflora in which gardnerella vaginalis; anaerobic species primarily among prevotella, porphyromonas, bacteroides, peptostreptococcus, and mobiluncus; mycoplasma hominis; and ureaplasma urealyticum become predominant in vaginal secre ...19989722706
anaerobic bacteremia and fungemia in patients undergoing endodontic therapy: an overview.oral focal infection, a concept neglected for several decades, is a subject of controversy. recent progress in classification and identification of oral microorganisms has renewed interest in focal infection. the aim of this study was to use phenotypic and genetic methods to trace microorganisms released into the bloodstream during and after endodontic treatment back to their presumed source--the root canal. microbiological samples were taken from the root canals of 26 patients with asymptomatic ...19989722712
early-onset periodontitis associated with down's syndrome--clinical interventional study.individuals with down's syndrome (ds) have an increased prevalence of periodontal disease compared with otherwise normal, age-matched control groups and other mentally handicapped patients of similar age distribution. the exaggerated immune-inflammatory response of the tissues cannot be explained by poor oral hygiene alone and might be the result of an impaired cell-mediated and humoral immunity and a deficient phagocytic system. as far as the progression and severity of destruction, the oral ma ...19989722720
analysis of ph-driven disruption of oral microbial communities in vitro.previously, a mixed culture chemostat system was used to demonstrate that the ph generated from carbohydrate metabolism, rather than carbohydrate availability per se, was responsible for the shifts observed in the oral microflora which are associated with high carbohydrate diets and the development of dental caries. the aim of this study was to determine more accurately the microbially generated ph at which such shifts occurred. nine oral bacteria were grown in three independent chemostats, and ...19989745120
role of fusobacterium nucleatum and coaggregation in anaerobe survival in planktonic and biofilm oral microbial communities during aeration.coaggregation is a well-characterized phenomenon by which specific pairs of oral bacteria interact physically. the aim of this study was to examine the patterns of coaggregation between obligately anaerobic and oxygen-tolerant species that coexist in a model oral microbial community. obligate anaerobes other than fusobacterium nucleatum coaggregated only poorly with oxygen-tolerant species. in contrast, f. nucleatum was able to coaggregate not only with both oxygen-tolerant and other obligately ...19989746571
identification and analyses of periodontal pathogens in taiwan by microbiological tests.the purpose of this study was to use microbiological tests for diagnosis of periodontal diseases in taiwan. anaerobic culture, direct microscopy, indirect immunofluorescence (if), and biochemical tests were used to examine 336 samples for the specific microorganisms in subgingival plaque. the results indicated that gram-negative species and motile bacteria were less frequently detected, and in lower proportion, in samples from healthy sites. the bacteria found frequently in healthy group were th ...19949747334
cytotoxic effect of endodontic bacteria on periapical fibroblasts.this study was conducted to investigate the effects of sonicated bacterial extracts (sbes) from anaerobic gram-negative bacteria on periapical fibroblast obtained from the apical portion of human periodontal ligaments. porphyromonas endodontalis, porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum were chosen from among the endodontic bacteria isolated from root canals having a periapical lesion and compared in terms of their cytotoxicity. the purpose of this study was t ...19989759015
sinusitis in neurologically impaired children.the microbiologic features of infected sinus aspirates in nine children with neurologic impairment were studied. anaerobic bacteria, always mixed with aerobic and facultative bacteria, were isolated in 6 (67%) aspirates and aerobic bacteria only in 3 (33%). there were 24 bacterial isolates, 12 aerobic or facultative and 12 anaerobic. the predominant aerobic isolates were klebsiella pneumoniae, escherichia coli, and staphylococcus aureus (2 each) and proteus mirabilis, pseudomonas aeruginosa, hae ...19989781990
microbiological findings in prepubertal periodontitis. a case report.generalized pre-pubertal periodontitis (gpp) is a rare entity that usually affects children with severe systemic diseases. we report the case of a 7-year-old male patient diagnosed with gpp, with no apparent systemic condition, who lost all his primary teeth to periodontal disease. before extractions, while he was still in mixed dentition the subgingival plaque was collected and analyzed using dna probes to 40 different microorganisms. putative periodontopathogens such as prevotella intermedia, ...19989802719
functional changes in thp-1 human monocytic cells after stimulation with lipopolysaccharide of oral microorganisms and granulocyte macrophage colony stimulating factor.a human thp-1 monocyte cell line culture system has been utilized to observe the effect of granulocyte macrophage colony stimulating factor (gm-csf) supplementation with lipopolysaccharide (lps) of oral microorganisms to stimulate monocyte/macrophage functional activity. lps of oral microorganisms, fusobacterium nucleatum and porphyromonas gingivalis was produced by phenol-water extraction and characterized. the phagocytosis assay was performed using f1tc labeled saccharomyces yeast particles. p ...19989805230
bacterial eradication from root dentine by ultrasonic irrigation with sodium hypochlorite.the study aimed to evaluate intracanal irrigation procedures in eradicating bacteria from surface, shallow and deep layers of root dentine using extracted human teeth. artificial bacterial smear layer was successfully produced by rubbing a mixture of dental plaque and artificially decalcified dentine or carious dentine on root canal walls. the reservoir holes were 3.5 mm in depth, 1 mm in diameter prepared 1.5 mm apart and parallel to the root canals on the decrowned planes, in which five separa ...19989823113
polymicrobial coronal leakage of super eba root-end fillings following two methods of root-end preparation.the aim of this study was to compose in vitro coronal leakage of a super eba root-end filling material after two root-end cavity preparation techniques. a mixed anaerobic microbial marker was used. forty-five extracted human teeth with straight, single root canals were prepared chemo-mechanically to a size 40 master apical file. the teeth were divided into experimental groups (35 teeth) and control groups (10 teeth). forty teeth (35 experimental teeth and five negative control teeth) were obtura ...19989823138
[a comparative in-vitro study of the bactericidal efficacy of sodium hypochlorite and chlorhexidine gluconate plus cetrimide on root canal anaerobic bacterial flora].the permanence of necrotic tissues and/or bacteria may be responsible for persistent root canal infection. root canal irrigation plays an important role in the success of endodontic treatment given that, on the one hand, it encourages the progressive elimination of the smear layer and, on the other, it neutralises microbic flora within the canal. the aim of this study was to test root canal irrigants from a microbiological point of view: sodium hypochlorite 5% (niclor ogna, milan) and a new gene ...19989835745
additional clinical and microbiological effects of amoxicillin and metronidazole after initial periodontal therapy.the aims of this study were to evaluate the clinical and microbiological effects of initial periodontal therapy (it) and to determine the additional effects of systemic amoxicillin (flemoxin solutab) 375 mg tid plus metronidazole 250 mg tid therapy, in patients with adult actinobacillus actinomycetemcomitans (aa)-associated periodontitis in conjunction with either porphyromonas gingivalis (pg), bacteroides forsythus (bf) and/or prevotella intermedia (pi). in addition the adverse effects of the a ...19989846793
septic arthritis due to fusobacterium nucleatum. 19989851282
preterm labor and bacterial intraamniotic infection: arachidonic acid liberation by phospholipase a2 of fusobacterium nucleatum.the studies presented in this report were undertaken to evaluate whether fusobacterium nucleatum, a common anaerobic isolate in intrauterine infection, stimulates arachidonic acid metabolism, a rate-limiting step for prostaglandin synthesis, in the human uterine endometrium.19989855600
analysis of serum antibody responses to periodontopathogens in early-onset periodontitis patients from different geographical locations.serum antibody specificity to oral micro-organisms was used to delineate the pathogens associated with early-onset periodontal diseases in a turkish population. additionally, comparison of the findings to those derived from a clinically similar us patient population described differences in bacterial specific antibody between these 2 geographic regions. serum from 89 (ljp), 86 (rpp) and 94 (normal) subjects was analyzed (elisa) to determine igg antibody to 14 oral micro-organisms. all ljp patien ...19989869349
identification of highly fucosylated n-linked oligosaccharides from the human parotid gland.the glycosylation of a number of constituents of human saliva is known to modify its biological roles, such as its lubricating properties and binding of microbial flora. gillece-castro et al. [gillece-castro, b. l., prakobphol, a., burlingame, a. l., leffler, h. & fisher, s. j. (1991) j. biol. chem. 266, 17358-17368] have proposed that the major glycan on the salivary proline-rich glycoproteins is a trifucosylated biantennary sugar with one difucosylated and one unfucosylated antenna. furthermor ...19989874230
preparation and characterization of an actinomyces naeslundii aggregation factor that mediates coaggregation with porphyromonas gingivalis.intergeneric coaggregation is responsible for the complexity of the microbiota in human dental plaque and is believed to be important in the initial bacterial colonization of the human oral cavity. actinomyces naeslundii, an early colonizer of the tooth surface, may enhance subsequent colonization by porphyromonas gingivalis which is associated with adult periodontitis. the purpose of this study was to isolate and characterize the a. naeslundii aggregation factor (anaf) that mediates coaggregati ...19989879519
oral microbial flora in sjögren's syndrome.to study presence of microorganisms associated with caries, gingivitis, oral opportunistic infections, and extent of hyposalivation in dentate patients with primary and secondary sjögren's syndrome (ss).19999918250
coronal leakage: endotoxin penetration from mixed bacterial communities through obturated, post-prepared root canals.coronal leakage has been considered as one of the major causes of failure in root canal treatment. bacteria have been shown to penetrate the root canal obturating materials and influence the periapical tissues. endotoxin, a component of gram-negative bacteria, is a potent inflammatory agent and may be able to penetrate obturating materials faster than bacteria. this in vitro study examined the possible penetration of post-prepared canals by endotoxin and bacterial cells derived from mixed bacter ...19989922745
unexpected cross-reaction with fusobacterium necrophorum in a pcr for detection of mycoplasmas.cross-reactions with fusobacterium necrophorum were found in a pcr designed for detection of a wide range of mycoplasma species. twenty-five strains of fusobacterium were examined; all 14 f. necrophorum strains reacted positively, whereas all 7 fusobacterium nucleatum strains reacted negatively. two strains that were not f. necrophorum yielded variable results.19999986867
elastase activity of anaerobes isolated from amniotic fluid with preterm premature rupture of membranes.a total of 131 anaerobes isolated from amniotic fluid with preterm premature rupture of membranes and stored were examined for elastolytic activity by the method described by williams et al (lett appl microbiol 1988;7:173-6).19999988804
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