| sonoporation is an efficient tool for intracellular fluorescent dextran delivery and one-step double-crossover mutant construction in fusobacterium nucleatum. | studies of microorganisms are often hindered by a lack of effective genetic tools. one such example is fusobacterium nucleatum, a gram-negative anaerobe associated with various human infections, including those causing periodontal disease and preterm birth. the first double-crossover allelic-exchange mutant in f. nucleatum was recently constructed using sonoporation, a novel ultrasound-mediated intracellular delivery method, demonstrating potential for bacterial gene transfection. to better unve ... | 2007 | 17449701 |
| comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction. | the outcome of microbiological diagnostics may depend on the sampling technique. it was the aim of the present study to compare two widely used sampling techniques for subgingival bacteria using quantitative real-time polymerase chain reaction. | 2007 | 17470026 |
| in vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. | propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. in the present study, the antimicrobial activity of five propolis samples, collected from four different regions in turkey and from brazil, against nine anaerobic strains was evaluated. ethanol extracts of propolis (eep) were prepared from propolis samples and we determined minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) of eep ... | 2007 | 17475517 |
| activation of toll-like receptors 2 and 4 by gram-negative periodontal bacteria. | periodontitis is a chronic infectious disease associated with a gram-negative subgingival microflora. bacterial components stimulate, among other receptors, toll-like receptor (tlr) 2 and/or tlr4. accumulating evidence indicates that both qualitatively and quantitatively distinct immune responses result from the triggering of tlr2 as compared to tlr4 triggering. the aim was to study the interaction of porphyromonas gingivalis, actinobacillus actinomycetemcomitans, tannerella forsythensis, prevot ... | 2007 | 17488439 |
| infection at titanium implants with or without a clinical diagnosis of inflammation. | to assess the microbiota at implants diagnosed with peri-implantitis, implant mucositis, or being clinically healthy. | 2007 | 17517058 |
| periodontal bacterial dna suppresses the immune response to mutans streptococcal glucosyltransferase. | certain cpg motifs found in bacterial dna enhance immune responses through toll-like receptor 9 (tlr-9) and may also demonstrate adjuvant properties. our objective was to determine if dna from bacteria associated with periodontal disease could affect the immune response to other bacterial antigens in the oral cavity. streptococcus sobrinus glucosyltransferase (gtf), an enzyme involved in dental caries pathogenesis, was used as a test antigen. rowett rats were injected with aluminum hydroxide (al ... | 2007 | 17517867 |
| odontogenic bacteria in periodontal disease and resistance patterns to common antibiotics used as treatment and prophylaxis in odontology in spain. | resistance in streptococci or gram-negative bacteria is associated with antibiotic consumption. scarce information exists on the antibiotic susceptibility of bacterial isolates from patients with periodontitis in countries with high antibiotic consumption, as this is an area in which microbiological testing is not performed in daily practice. the present study was undertaken to explore the susceptibility of bacterial isolates in periodontitis to antibiotics prescribed in odontology in spain as t ... | 2007 | 17530037 |
| effect of an essential oil-containing antimicrobial mouthrinse on specific plaque bacteria in vivo. | this study was conducted to investigate the effect of rinsing with an essential oil-containing mouthrinse on levels of specific supra and subgingival bacteria in subjects with gingivitis. | 2007 | 17535287 |
| bacterial species in subgingival plaque and oral bone loss in postmenopausal women. | oral bacteria are widely recognized in the etiology of periodontal disease. we investigated the prevalence of subgingival bacterial infection with eight species, tested associations between infection and oral bone loss, and assessed potential confounding factors and effect modifiers of those associations in a large community-based cohort of postmenopausal women. | 2007 | 17539719 |
| co-adhesion and biofilm formation by fusobacterium nucleatum in response to growth ph. | fusobacterium nucleatum is a gram-negative anaerobic organism considered to play an important role in the progression of periodontal disease and is commonly found in clinical infections of other body sites. apart from its metabolic versatility, its cell-surface properties enable it to attach to epithelial cells, collagen, gingival epithelial cells and other bacterial genera, but not with other fusobacteria. the development of periodontitis is associated with a rise in ph in the gingival sulcus t ... | 2007 | 17540586 |
| a novel tem contrasting technique for extracellular polysaccharides in in vitro biofilms. | in the past, the visualization of the extracellular matrix of biofilms on an ultrastructural level was hampered by shrinkage artifacts. in addition, the reproducible contrasting of extracellular polysaccharides (eps) has not satisfactorily been solved. here we describe a method overcoming these difficulties, which produces artifact-free transmission electron microscopic (tem) images using multispecies biofilms grown in vitro. sufficient contrast was achieved by replacing schiff's reagent with th ... | 2007 | 17557287 |
| activation of protective responses in oral epithelial cells by fusobacterium nucleatum and human beta-defensin-2. | oral epithelia are constantly exposed to non-pathogenic (commensal) bacteria, but generally remain healthy and uninflamed. fusobacterium nucleatum, an oral commensal bacterium, strongly induces human beta-defensin-2 (hbd2), an antimicrobial and immunomodulatory peptide, in gingival epithelial cells (gecs). hbd2 is also expressed in normal oral tissue leading to the hypothesis that oral epithelia are in an activated state with respect to innate immune responses under normal in vivo conditions. in ... | 2007 | 17577065 |
| physiological alterations of a fusobacterium nucleatum strain exposed to oxidative stress. | the purpose of this study was to investigate the effect of oxidative stress on physiological and genetic characteristics of fusobacterium nucleatum and its interference on this microbial identification methods. | 2007 | 17584449 |
| production of antagonistic substance by eikenella corrodens isolated from the oral cavity of human beings with and without periodontal disease. | antagonistic abilities may confer ecological advantages for micro-organisms in competitive ecosystems. however, reports regarding this phenomenon in eikenella corrodens are not available. | 2007 | 17584471 |
| fada from fusobacterium nucleatum utilizes both secreted and nonsecreted forms for functional oligomerization for attachment and invasion of host cells. | fusobacterium nucleatum is a gram-negative anaerobe associated with various human infections, including periodontal diseases and preterm birth. a novel fada adhesin was recently identified for host-cell binding. it consists of 129 amino acid residues, with an 18-amino acid signal peptide. expression of fada in escherichia coli enhanced bacterial binding to host epithelial and endothelial cells. in both e. coli and f. nucleatum, fada exists in two forms, the intact pre-fada and the secreted matur ... | 2007 | 17588948 |
| association of a high-molecular weight arginine-binding protein of fusobacterium nucleatum atcc 10953 with adhesion to secretory immunoglobulin a and coaggregation with streptococcus cristatus. | fusobacterium nucleatum coaggregates with a diverse range of bacterial species, and binds to host tissues and proteins such as immunoglobulin. these interactions may support the attachment of a variety of organisms to oral surfaces and can facilitate the invasion of soft tissues. we hypothesized that coaggregation with streptococci and immunoglobulin binding may occur by a common adhesin sensitive to l-arginine. | 2007 | 17600532 |
| cultivable bacteria in infected root canals as identified by 16s rrna gene sequencing. | introduction: traditionally, cultivable bacteria isolated from infected root canals have been identified by phenotype-based methods. because 16s ribosomal rna (rrna) gene sequencing has emerged as a more accurate and reliable tool for bacterial identification, the present study applied this approach to identify bacterial isolates recovered from the root canals of teeth with chronic apical periodontitis. methods: anaerobic techniques were used for culturing; identification of the isolates was car ... | 2007 | 17600539 |
| femoral vein thrombophlebitis and septic pulmonary embolism due to a mixed anaerobic infection including solobacterium moorei: a case report. | abstract: | 2007 | 17605768 |
| the effect of mineral trioxide aggregate on phagocytic activity and production of reactive oxygen, nitrogen species and arginase activity by m1 and m2 macrophages. | to assess the influence of co-culture with mineral trioxide aggregate (mta) on phagocytosis and the production of reactive oxygen intermediates (roi) and nitrogen (no) species and the arginase activity by m1 and m2 peritoneal macrophages. | 2007 | 17627697 |
| development of a genus-specific pcr assay for the molecular detection, confirmation and identification of fusobacterium spp. | a genus-specific polymerase chain reaction (pcr)-based assay is developed for the detection and identification of clinically relevant fusobacterium species, including f. nucleatum and f. necrophorum. two 16s ribosomal dna (rdna) primers, fuso1 (forward primer: 5'-gag aga gct ttg cgt cc-3' [17-mer]) and fuso 2 (reverse primer: 5'-tgg gcg ctg agg ttc gac -3' [18-mer]) are designed to target conserved regions of the 16s rdna gene for fusobacterium spp. subsequent proof-of-principle studies employin ... | 2007 | 17633142 |
| [lemierre syndrome]. | | 2007 | 17633598 |
| role of anaerobes in acute pelvic inflammatory disease. | pouch of douglas aspirates were collected from 50 women with history and examination suggestive of acute pelvic inflammatory disease (pid) and 20 healthy women admitted for tubal ligation served as control. a total of 57 microorganisms were isolated from 37 patients out of 50 in study group. of 37 positive cultures 21(56.7%) were monomicrobial and 16(43.2%) were polymicrobial. most common symptom in study group was lower abdominal pain (90%), vaginal discharge (70%) and irregular bleeding (40%) ... | 2003 | 17643017 |
| targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time pcr. | an in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. in this study, a quantitative taqman pcr assay (qpcr) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofil ... | 2007 | 17653931 |
| genome sequence of fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium. | fusobacterium nucleatum is a prominent member of the oral microbiota and is a common cause of human infection. f. nucleatum includes five subspecies: polymorphum, nucleatum, vincentii, fusiforme, and animalis. f. nucleatum subsp. polymorphum atcc 10953 has been well characterized phenotypically and, in contrast to previously sequenced strains, is amenable to gene transfer. we sequenced and annotated the 2,429,698 bp genome of f. nucleatum subsp. polymorphum atcc 10953. plasmid pfn3 from the stra ... | 2007 | 17668047 |
| effects of glass ionomer and microfilled composite subgingival restorations on periodontal tissue and subgingival biofilm: a 6-month evaluation. | this 6-month study evaluated the effects of resin-modified glass-ionomer cement (rmgi) and microfilled composite (mc) subgingival restorations on periodontal tissues and subgingival biofilm. | 2007 | 17668971 |
| fusobacterium nucleatum induces fetal death in mice via stimulation of tlr4-mediated placental inflammatory response. | intrauterine infection plays a pivotal role in preterm birth (ptb) and is characterized by inflammation. currently, there is no effective therapy available to treat or prevent bacterial-induced ptb. using fusobacterium nucleatum, a gram-negative anaerobe frequently associated with ptb, as a model organism, the mechanism of intrauterine infection was investigated. previously, it was shown that f. nucleatum induced preterm and term stillbirth in mice. fusobacterial-induced placental infection was ... | 2007 | 17675512 |
| hemoglobin and lps act in synergy to amplify the inflammatory response. | vascular disruption and bleeding during periodontitis likely increase the levels of hemoglobin in gingival crevicular fluid. the aim of this study was to investigate the effect of hemoglobin on the inflammatory responses of human macrophages stimulated with lipopolysaccharides (lps) isolated from periodontopathogens. the production of interleukin-1 beta (il-1beta), il-6, il-8, and tumor necrosis factor alpha (tnf-alpha) by macrophages following challenges with porphyromonas gingivalis and fusoba ... | 2007 | 17720859 |
| the impact of a low-frequency chlorhexidine rinsing schedule on the subgingival microbiota (the teeth clinical trial). | information on the efficacy of chlorhexidine (chx) rinsing on the subgingival microbiota is limited. this study tested if intermittent chx rinsing over 5 years had an impact on the subgingival microbiota. | 2007 | 17760545 |
| functionalization of pvdf membranes with carbohydrate derivates for the controlled delivery of chlorhexidin. | maltodextrin (mx) was fixed onto pvdf membranes in order to create a drug delivery guided tissue regeneration (gtr) device with controlled drug delivery properties. pvdf microporous membranes were treated by a mixture of mx and citric acid, resulting to an 18 wt% increase of the supports. mx grafted membrane could capture 103 mg/g chlorhexidin digluconate (digchx) instead of 1mg/g for a virgin membrane. a neutralization step was performed before the biological tests. viability tests confirmed th ... | 2007 | 17804290 |
| comparison of growth and susceptibility to sodium hypochlorite of mono- and dual-species biofilms of fusobacterium nucleatum and peptostreptococcus (micromonas) micros. | this study compared growth and susceptibility to different concentrations of sodium hypochlorite (naocl) of mono- and dual-species biofilms of fusobacterium nucleatum or peptostreptococcus micros in vitro at 24 hours or 96 hours. a mann-whitney u test revealed that although at 24 hours dual-species biofilms had similar viable counts to those of monospecies biofilms (p > 0.001), they were more resistant to naocl (p < 0.001). at 96 hours, both microorganisms had higher viable counts and were more ... | 2007 | 17804319 |
| [application of "microring an" to "level 1": presumptive identification of anaerobic gram-negative bacilli]. | commonly isolated anaerobic gram-negative rods (4 genus 64 strains), some other important gram-negative anaerobic species (9 genus 45 strains), and cigar-shaped clostridia (11 strains) were studied on their susceptibility patterns to 6 agents on "microring an". some modifications were made in the methods and interpretation of results. susceptibility patterns to erythromycin, rifampicin, colistin, benzylpenicillin, kanamycin, and vancomycin were following (sensitive [s], intermediate [i], resista ... | 2006 | 17822332 |
| antimicrobial effect of triclosan and triclosan with gantrez on five common endodontic pathogens. | microbial control of the root canal system is one of the key objectives of root canal therapy. triclosan is a widely accepted broad spectrum antimicrobial agent proven to be effective against many gram-positive and gram-negative bacteria. triclosan acts by blocking bacterial fatty acid biosynthesis. the addition of gantrez copolymer has been shown to enhance the antimicrobial activity of triclosan. the purpose of this study was to determine the minimum inhibitory and bactericidal concentrations ... | 2007 | 17889698 |
| implant surface analysis and microbiologic evaluation of failed implants retrieved from smokers. | the aim of this study was to evaluate the microbiota and surface of failed titanium dental implants from 4 manufacturers. twelve mobile dental implants were retrieved from 10 smokers after 3 to 10 years of functional loading. before implant removal, microbial samples were taken and evaluated using polymerase chain reaction. after implant removal, analyses of the failed implant surfaces were performed using scanning electron microscopy and energy-dispersive spectrometer x-ray. periodontal pathoge ... | 2007 | 17912965 |
| the trimeric periplasmic chaperone skp of escherichia coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactions. | the interactions of outer membrane proteins (omps) with the periplasmic chaperone skp from escherichia coli are not well understood. we have examined the binding of skp to various omps of different origin, size, and function. these were ompa, ompg, and yaet (omp85) from escherichia coli, the translocator domain of the autotransporter nalp from neisseria meningitides, foma from fusobacterium nucleatum, and the voltage-dependent anion-selective channel, human isoform 1 (hvdac1) from mitochondria. ... | 2007 | 17928002 |
| bacterial leakage along the implant-abutment interface of premachined or cast components. | in recent clinical studies, contamination of the inner parts of dental implants through bacterial penetration along the implant components has been observed. the aim of the present in-vitro study was to investigate leakage of fusobacterium nucleatum through the interface between implants and premachined or cast abutments. both premachined (n=10) and cast (n=10) implant-abutment assemblies were inoculated with 3.0 microl of microbial inoculum. the assemblies were completely immersed in 5.0 ml of ... | 2008 | 17931833 |
| compressed mints and chewing gum containing magnolia bark extract are effective against bacteria responsible for oral malodor. | flavors and natural botanic extracts are often used in chewing gum and compressed mints for breath freshening and relief of oral malodor. the oral malodor is a result of bacterial putrification of proteinaceous materials from food or saliva. in this study, magnolia bark extract (mbe) and its two main components, magnolol and honokiol, were evaluated by the minimum inhibition concentration (mic) test. the inhibitory effect of mbe mint was further evaluated by a kill-time assay study. in addition, ... | 2007 | 17949053 |
| evaluation of the microbiota of primary endodontic infections using checkerboard dna-dna hybridization. | the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections in 111 selected cases of single-rooted teeth with necrotic pulp. | 2007 | 17949342 |
| improved accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standard definition. | terminal restriction fragment length polymorphism (t-rflp) analysis is commonly used to analyze microbial communities, including oral microflora. however, accurate identification of terminal restriction fragment (t-rf) origins is prevented by unpredictable errors in sizing, thus necessitating the clone library analysis. to minimize sizing errors, we proposed optimizing the size definition of internal standards. | 2007 | 17949346 |
| antimicrobial profiles of prevotella spp. and fusobacterium nucleatum isolated from periodontal infections in a selected area of southern italy. | in view of the increasing antibiotic resistance of anaerobic gram-negative bacteria, we determined the antimicrobial profile of 55 periodontal anaerobic gram-negative bacteria correlated with human infections, comprising 16 strains of fusobacterium nucleatum and 39 strains of prevotella spp. isolated from periodontal pockets of 26 adults suffering from chronic periodontitis. all the strains of f. nucleatum were susceptible to amoxicillin/clavulanic acid, doxycycline, metronidazole, moxifloxacin ... | 2007 | 17954025 |
| innate immune responses of gingival epithelial cells to nonperiodontopathic and periodontopathic bacteria. | we have previously reported different susceptibilities of periodontopathic and nonperiodontopathic bacteria to antimicrobial peptides and phagocytosis by neutrophils. differences between the two groups of bacteria may exist also in their ability to induce immune responses from the host. therefore, we evaluated the effects of various oral bacteria on innate immune responses by gingival epithelial cells. | 2007 | 17956462 |
| inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of porphyromonas gingivalis. | the purpose of this study was to investigate the effects of cranberry polyphenol fraction on biofilm formation and activities of arg-gingipain and lys-gingipain in porphyromonas gingivalis. | 2007 | 17956474 |
| antimicrobial effect of propolis and other substances against selected endodontic pathogens. | the aim of this study was to determine the antimicrobial effect of ethanol extract of propolis (eep) and intracanal medicaments calcium hydroxide, camphorated paramonochlorophenol, and formocresol by means of the macrodilution method using the reinforced clostridial medium (rcm) and brucella and brain heart infusion media. | 2007 | 17964476 |
| involvement of phospholipase d in regulating expression of anti-microbial peptide human beta-defensin-2. | human beta-defensin expression correlates with differentiation in oral epithelium, and calcium ion, an important regulator of epithelial differentiation, plays a critical role in regulation of human beta-defensin-2 (hbd-2) mrna expression. phospholipase d (pld) also regulates epithelial differentiation. therefore, we examined the role of pld in hbd-2 up-regulation by cell wall extract of fusobacterium nucleatum and phorbol 12-myristate 13-acetate (pma), two known hbd-2 activators. we found that ... | 2008 | 17986621 |
| the inhibition of dentifrice containing the lotus leaf-derived inhibitor on periodontitis-related bacteria in vitro. | to search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease. | 2007 | 17992914 |
| [the development and in vitro experiment study of a bio-type root canal filling sealer using calcium phosphate cement]. | the purpose of this study is to develop a novel root canal filling sealers based on calcium phosphate cement (cpc), and to evaluate its physical-chemical properties and in vitro antibacterial activity on the predominant bacteria infecting root canal. | 2007 | 18004487 |
| soluble cd14 discriminates slight structural differences between lipid as that lead to distinct host cell activation. | soluble cd14 (scd14) in serum is known to sensitize host cells to lps. in the present study, the contributions of scd14 and lps-binding protein to a lipid a moiety from lps preparations of periodontopathogenic fusobacterium nucleatum sp. nucleatum were compared with that of escherichia coli-type synthetic lipid a (compound 506). f. nucleatum lipid a was identified to be a hexa-acylated fatty acid composed of tetradecanoate (c(14)) and hexadecanoate (c(16)), similar to dodecanoate (c(12)) and c(1 ... | 2007 | 18025213 |
| effect of chlorhexidine/thymol and fluoride varnishes on dental biofilm formation in vitro. | this study evaluated the effect of chlorhexidine/thymol (chx/t) and fluoride (f) varnishes on biofilm formation in vitro. hydroxyapatite discs coated with varnish were first immersed in saline for 0, 3, 7 or 14 d, then immersed in pasteurized saliva. the discs were incubated for 20 h with a bacterial suspension containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, and veillonella dispar. uncoated discs were used as controls. growth of bacteria on the discs was evaluat ... | 2007 | 18028054 |
| improved diagnosis specificity in bone and joint infections using molecular techniques. | the microbiological diagnosis of osteoarticular infections currently relies on microbiological cultures, to specifically target treatment. however, these conventional methods sometimes lack of sensitivity and of specificity to establish definitive diagnosis. this study was conducted to determine whether molecular method could improve bacterial bone and joint infection diagnosis. | 2007 | 18029022 |
| [case report: positive signals from automated blood-culture system with negative direct gram examination]. | detection of positive haemoculture is usually managed by an automated system. when a bottle is detected positive but that the gram coloration does not reveal germs by direct examination, transfer onto chocolate blood agar generally allows to confirm or infirm bacteraemia. in light of a case of fusobacterium nucleatum bacteraemia, we discuss the opportunity of pairing it with an enrichment broth. m. n, hospitalized in the hepatogastroenterology department, runs a fever of undetermined origin. thr ... | 2007 | 18031953 |
| effect of tissue fluids on hydrophobicity and adherence of enterococcus faecalis to dentin. | this in vitro study was carried out to determine (1) the hydrophobicity of selected oral bacteria, (2) the influence of growth media (saliva and serum) and mode of growth (planktonic or biofilm) on the hydrophobicity of enterococcus faecalis, and (3) the influence of growth media and conditioning fluids on the adherence of e. faecalis to dentin. the ability to bind to a hydrocarbon phase (xylene) was used as an index of relative hydrophobicity of cells. fluorescent microscopy-based technique was ... | 2007 | 18037050 |
| early soft tissue healing around one-stage dental implants: clinical and microbiologic parameters. | despite the potential significance of early soft tissue healing to long-term outcomes, this aspect of one-stage dental implants has not been investigated. the purpose of this prospective study was to characterize clinical and microbiologic parameters of early soft tissue healing around dental implants placed following a one-stage protocol. | 2007 | 18062110 |
| effect of rinsing with an essential oil-containing mouthrinse on subgingival periodontopathogens. | studies have shown that the nature and amount of supragingival plaque can influence the composition of the contiguous subgingival flora. the purpose of this study was to investigate the effect of rinsing with an essential oil antimicrobial mouthrinse on levels of representative subgingival bacteria in subjects with mild to moderate periodontitis. | 2007 | 18062115 |
| analysis of the antimicrobial activity of local anaesthetics used for dental analgesia. | seven local anaesthetics and their active anaesthetic components [ultracaine d-s (articaine hydrochloride), carbostesin (bupivacaine hydrochloride), scandicaine (mepivacaine hydrochloride), xylonest (prilocaine hydrochloride), xylocaine (lidocaine hydrochloride), hostacaine (butanilicaine phosphate) and novocaine (procaine hydrochloride)] were tested for their antimicrobial activity against 311 bacterial strains from 52 different species and 14 candida albicans strains. the tested pathogens were ... | 2008 | 18065672 |
| antimicrobial activity of mahonia aquifolium and two of its alkaloids against oral bacteria. | extracts or alkaloids isolated from mahonia aquifolium exhibit antimicrobial activity against gram-positive and gram-negative bacteria, fungi, and protozoa. in this study the bacteriostatic and bacteriocidal activities of a m. aquifolium extract and two of its major alkaloids, berberine chloride and oxyacanthine sulphate, were tested in vitro against nine different oral bacteria. minimum inhibitory concentrations were in the range from < or = 0.0031% to 0.1993% for the m. aquifolium extract, fro ... | 2007 | 18072463 |
| effect of photo-activated disinfection on endodontic pathogens ex vivo. | to test the hypothesis that photo-activated disinfection (pad) has a bactericidal effect on pathogens inoculated in root canals, with emphasis on biofilm formation/destruction. | 2008 | 18081808 |
| quantitative analysis of association between herpesviruses and bacterial pathogens in periodontitis. | the development of human periodontitis may depend upon cooperative interactions among herpesviruses, specific pathogenic bacteria and tissue-destructive inflammatory mediators. this study sought to identify associations among human cytomegalovirus, epstein-barr virus and six putative periodontopathic bacteria in periodontitis lesions. | 2008 | 18086168 |
| stimulation of fusobacterium nucleatum biofilm formation by porphyromonas gingivalis. | bacterial infection is a major cause of periapical periodontitis. eradication of these microorganisms from apical lesions is essential to the success of endodontic treatment. the aim of this study was to clarify the molecular interaction between fusobacterium nucleatum, porphyromonas gingivalis and other microorganisms associated with periapical periodontitis. | 2008 | 18173791 |
| proteomic analysis of ampicillin-resistant oral fusobacterium nucleatum. | fusobacterium nucleatum represents one of the predominant anaerobic species in the oral microbiota. penicillin-resistant f. nucleatum have been isolated from intra- and extraoral infections. this study aimed to assess ampicillin resistance in f. nucleatum by investigating the synthesis of resistance-associated proteins. | 2008 | 18173796 |
| relationship between campylobacter rectus and periodontal status during pregnancy. | in a previous study, we showed that the growth of campylobacter rectus is stimulated by the presence of female sex hormones in the culture medium. in the present study, we examined the relationship between c. rectus levels in the saliva and the periodontal status of pregnant women. | 2008 | 18173799 |
| microflora in oral ecosystems in subjects with radiation-induced hyposalivation. | to analyse the microbial flora in specific oral sites in 13 dentate subjects, 6-8 months after completed radiation therapy (rt group) and in 13 matched controls. | 2008 | 18208474 |
| genetic and biochemical characterization of oxa-63, a new class d beta-lactamase from brachyspira pilosicoli bm4442. | brachyspira pilosicoli bm4442, isolated from the feces of a patient with diarrhea at the hospital saint-michel in paris, was resistant to oxacillin (mic > 256 microg/ml) but remained susceptible to cephalosporins and to the combination of amoxicillin and clavulanic acid. cloning and sequencing of the corresponding resistance determinant revealed a coding sequence of 807 bp encoding a new class d beta-lactamase named oxa-63. the bla oxa-63 gene was chromosomally located and not part of a transpos ... | 2008 | 18212108 |
| in vitro antioxidant potential of medicinal plant extracts and their activities against oral bacteria based on brazilian folk medicine. | this study aims to determine antibacterial activities of cocos nucifera (husk fiber), ziziphus joazeiro (inner bark), caesalpinia pyramidalis (leaves), aqueous extracts and aristolochia cymbifera (rhizomes) alcoholic extract against prevotella intermedia, porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mutans and lactobacillus casei. the antioxidant activity and acute toxicity of these extracts were also evaluated. | 2008 | 18243157 |
| a case report of a pyogenic liver abscess caused by fusobacterium nucleatum in a patient with autosomal dominant polycystic kidney disease undergoing hemodialysis. | pyogenic liver abscess (pla) is a process with significant morbidity and mortality and is a rare complication in an aisled way in patients with autosomal dominant polycystic kidney disease (adpkd). in addition to hepatic cyst infection, intracystic hemorrhage is another complication seen in adpkd patients; however, the liver parenchyma itself remains normal. a pla located in normal liver tissue in these kinds of patients has not been previously reported. fusobacterium nucleatum is an anaerobic b ... | 2008 | 18257820 |
| smap29 congeners demonstrate activity against oral bacteria and reduced toxicity against oral keratinocytes. | cathelicidins are antimicrobial peptides found in epithelial and mucosal tissues as well as the secondary granules of neutrophils. smap29, a sheep cathelicidin, has differential antimicrobial properties against various pathogens, including periodontal organisms. the purpose of this study was to evaluate the antimicrobial properties and cytotoxicity of smap29, smap28, and three congeners (smap18a, smap18d, and smap14a). | 2008 | 18279175 |
| aggregatibacter actinomycetemcomitans serotype f o-polysaccharide mediates coaggregation with fusobacterium nucleatum. | intergeneric bacterial coaggregation may play an important role in plaque development. | 2008 | 18279180 |
| colonization pattern of periodontal bacteria in japanese children and their mothers. | the purpose of this study was to determine the time of infection by anaerobic gram-negative rods associated with periodontal disease, and to clarify their transmission from mother to child. | 2008 | 18302616 |
| interactions between salivary bifidobacterium adolescentis and other oral bacteria: in vitro coaggregation and coadhesion assays. | coaggregation assays were performed to investigate interactions between oral bifidobacterium adolescentis and other oral bacterial species. bifidobacterium adolescentis olb6410 isolated from the saliva of healthy humans did not coaggregate with actinomyces naeslundii jcm8350, streptococcus mitis ols3293, streptococcus sanguinis jcm5708, veillonella parvula atcc17745 or porphyromonas gingivalis ob7124, but it did coaggregate with fusobacterium nucleatum jcm8532. subsequent examination of biofilm ... | 2008 | 18312575 |
| genotypic diversity of anaerobic isolates from bloodstream infections. | accurate species determination for anaerobes from blood culture bottles has become increasingly important with the reemergence of anaerobic bacteremia and prevalence of multiple-drug-resistant microorganisms. our knowledge of the taxonomical diversity of anaerobes that cause bloodstream infections is extremely limited, because identification historically has relied on conventional methods. over a 5-year period, we profiled anaerobic bacteremia at a large tertiary care hospital with 16s rrna gene ... | 2008 | 18322067 |
| prevalence of periodontal pathogens in subgingival lesions, atherosclerotic plaques and healthy blood vessels: a preliminary study. | previous studies have reported different periodontal bacteria in atherosclerotic lesions, but their involvement in plaque formation remains unclear. the aim of the present study was to investigate the presence of 20 periodontal bacteria in atherosclerotic samples and healthy blood vessels (used as controls) and to clarify their relationship in regard to clinical and bacteriological periodontal status. | 2008 | 18326058 |
| thoracic vertebral actinomycosis: actinomyces israelii and fusobacterium nucleatum. | actinomyces spp. are considered rare pathogens in today's medicine, especially with thoracic vertebral involvement. classic actinomycosis (50%) presents as an oral-cervicofacial ("lumpy jaw") infection. this report describes a case of spinal cord compression caused by actinomyces israelii with the coisolation of fusobacterium nucleatum. there are limited numbers of similar cases. | 2008 | 18337385 |
| antimicrobial activity of sodium hypochlorite and chlorhexidine by two different tests. | the aim of this study was to evaluate the antimicrobial capacity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%) with or without the addition of organic material (bovine serum albumin, bsa) against some bacterial samples (staphylococcus aureus, enterococcus faecalis, escherichia coli, porphyromonas gingivalis and fusobacterium nucleatum) using two activity tests (contact and diffusion agar tests). in the contact test (first model), bacterial samples were kept in contact ... | 2008 | 18352899 |
| pyogenic liver abscess related to dental disease in an immunocompetent host. | a 59-year-old man with poor oral hygiene presented to our hospital because of fever and chills. abdominal ultrasonography and enhanced computed tomography (ct) revealed a liver abscess. the patient had no history of immunodeficiency and we confirmed the patient had no immunologic abnormalities. blood culture revealed fusobacterium nucleatum, a bacterium commonly found in the oral cavity. even if a patient is immunocompetent, poor oral hygiene might be an independent risk factor for a pyogenic li ... | 2008 | 18379160 |
| [detection of periodontal pathogens in coronary atherosclerotic plaques]. | to explore the possible correlation between coronary heart disease and periodontitis. | 2008 | 18380965 |
| bacterial profiles of root caries in elderly patients. | culture-based studies have shown that streptococcus mutans and lactobacilli are associated with root caries (rc). the purpose of the present study was to assess the bacterial diversity of rc in elderly patients by use of culture-independent molecular techniques and to determine the associations of specific bacterial species or bacterial communities with healthy and carious roots. plaque was collected from root surfaces of 10 control subjects with no rc and from 11 subjects with rc. the bacterial ... | 2008 | 18385433 |
| role of porphyromonas gingivalis serb in gingival epithelial cell cytoskeletal remodeling and cytokine production. | the serb protein of porphyromonas gingivalis is a had family serine phosphatase that plays a critical role in entry and survival of the organism in gingival epithelial cells. serb is secreted by p. gingivalis upon contact with epithelial cells. here it is shown by microarray analysis that serb impacts the transcriptional profile of gingival epithelial cells, with pathways involving the actin cytoskeleton and cytokine production among those significantly overpopulated with differentially regulate ... | 2008 | 18391005 |
| the lower genital tract microbiota in relation to cytokine-, slpi- and endotoxin levels: application of checkerboard dna-dna hybridization (cdh). | in the present study the lower genital tract microbiota in asymptomatic fertile women (n=34) was identified and quantified by culturing vaginal secretions. also, vaginal and cervical samples were analyzed by a semiquantitative checkerboard dna-dna hybridization technique (cdh) based on genomic probes prepared from 13 bacterial species (bacteroides ureolyticus, escherichia coli, fusobacterium nucleatum, gardnerella vaginalis, mobiluncus curtisii ss curtisii, prevotella bivia, prevotella disiens, ... | 2008 | 18397461 |
| bleeding on probing differentially relates to bacterial profiles: the oral infections and vascular disease epidemiology study. | various bacterial species are differentially prevalent in periodontal health, gingivitis or periodontitis. we tested the independent associations between three bacterial groupings and gingival inflammation in an epidemiological study. | 2008 | 18400025 |
| [microbiological and biochemical characteristics of inflammatory tissues in the periodontium]. | to investigate bacterial populations in subgingival and supragingival plaque samples of patients with inflammatory periodontal diseases and activities of the lysosomal enzymes--lysozyme, alkaline phosphatase, and beta-glucuronidase--in peripheral venous blood, in gingival crevicular fluid, and mixed nonstimulated saliva. | 2008 | 18413987 |
| microbiological evaluation of primary endodontic infections in teeth with and without sinus tract. | to examine the microbiological status of primary endodontic infections in teeth with and without a sinus tract. | 2008 | 18422583 |
| serum antibodies to porphyromonas gingivalis chaperone htpg predict health in periodontitis susceptible patients. | chaperones are ubiquitous conserved proteins critical in stabilization of new proteins, repair/removal of defective proteins and immunodominant antigens in innate and adaptive immunity. periodontal disease is a chronic inflammatory infection associated with infection by porphyromonas gingivalis that culminates in the destruction of the supporting structures of the teeth. we previously reported studies of serum antibodies reactive with the human chaperone hsp90 in gingivitis, a reversible form of ... | 2008 | 18431474 |
| a microbiological profile of symptomatic teeth with primary endodontic infections. | the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections associated with symptomatic teeth. samples were collected by means of a #15 h-type file and 2 sterile paper points from 60 symptomatic (n = 30) or asymptomatic (n = 30) single-rooted teeth with necrotic pulp. the presence of 40 bacterial species was determined by the checkerboard dna-dna hybridization method. the species found in higher counts (x10(5)) in symptomatic cases were fusobacterium ... | 2008 | 18436031 |
| impact of growth conditions on susceptibility of five microbial species to alkaline stress. | the effects of different growth conditions on the susceptibility of five taxa to alkaline stress were investigated. enterococcus faecalis atcc 29212, streptococcus sobrinus omz 176, candida albicans atcc 90028, actinomyces naeslundii atcc 12104, and fusobacterium nucleatum atcc 10953 were grown as planktonic cells, allowed to adhere to dentin for 24 hours, grown as monospecies or multispecies biofilms on dentin under anaerobic conditions with a serum-enriched nutrient supply at 37 degrees c for ... | 2008 | 18436038 |
| a rare presentation of ventriculitis and brain abscess caused by fusobacterium nucleatum. | anaerobic ventriculitis is rare, and usually seen in patients with predisposing factors such as otitis media, mastoiditis, sinusitis or recent neurosurgery. we report what we believe to be the first case of ventriculitis and brain abscess due to fusobacterium nucleatum infection in a man with no significant predisposing factors. he was successfully treated with antibiotic therapy. | 2008 | 18436604 |
| [prediction on the efficacy of fluoroquinolones for anaerobic infections using monte carlo simulation]. | various analysis based on pk-pd (pharmacokinetics-pharmacodynamics) theory were performed for prediction on the efficacy of antimicrobial agents for infectious diseases. however, there have been few reports when causative organisms would be anaerobic bacteria. although the antimicrobial activity of fluoroquinolones against anaerobes has been weak, gatifloxacin (gflx), moxifloxacin (mflx), and garenoxacin (grnx) are considered as anti-anaerobic quinolones in recent days. therefore, we investigate ... | 2007 | 18447205 |
| effects of age and oral disease on systemic inflammatory and immune parameters in nonhuman primates. | this report evaluated systemic inflammatory and immune biomarkers in a cohort of macaca mulatta (rhesus monkeys) maintained as a large family social unit, including an age range from <1 year to >24 years. we hypothesized that the systemic host responses would be affected by the age, gender, and clinical oral presentation of the population, each contributing to inflammatory and immune responses that would reflect chronic oral infections. the results demonstrated that the prevalence and severity o ... | 2008 | 18448617 |
| ability of oral bacteria to induce tissue-destructive molecules from human neutrophils. | the induction of tissue-destructive molecules from neutrophils by periodontopathic bacteria has been suggested as one of the mechanisms of periodontal destruction. the aim of this study was to determine whether the ability to stimulate neutrophils is an authentic characteristic of periodontopathic bacteria. | 2008 | 18449962 |
| photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial. | recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, currently, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of pdt in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. twenty-fou ... | 2009 | 18465191 |
| candida dubliniensis and candida albicans display surface variations consistent with observed intergeneric coaggregation. | adherence of yeasts to other microorganisms and epithelial cell surfaces is important in their colonization. comparative studies based on the coaggregation of candida dubliniensis versus candida albicans with fusobacterium nucleatum and other oral bacteria suggested differences in the surfaces of these yeasts. transmission electron microscopy was used to test the hypothesis that there are morphologic variations in the cell surface of these two species. c. dubliniensis type strain cd36 and c. alb ... | 1999 | 18473545 |
| [alterations of salivary flow rate and oral main pathogens in miniature pig with bilateral parotid gland atrophy]. | to observe the effect of bilateral parotid gland atrophy on the whole saliva flow rate and the growth of main oral pathogens in different sites of oral cavity. | 2007 | 18476559 |
| quercetin in a lotus leaves extract may be responsible for antibacterial activity. | in the course of a search for chemotherapeutic agents inhibiting suspected peridontitis bacteria, extracted and purified substances from lotus leaf were identified by antimicrobial activity tests with use of the broth micro-dilution methods on 96-microwell plate. the antimicrobial activity of extracts was tested against five microorganisms, namely: actinobacillus actinomycetemcomitans y4, actinomyces viscosus 19246, porphyromonas gingivalis 33277, fusobacterium nucleatum 25586, and actinomyces n ... | 2008 | 18481022 |
| photodynamic treatment of endodontic polymicrobial infection in vitro. | we investigated the photodynamic effects of methylene blue on multispecies root canal biofilms comprising actinomyces israelii, fusobacterium nucleatum subspecies nucleatum, porphyromonas gingivalis, and prevotella intermedia in experimentally infected root canals of extracted human teeth in vitro. the 4 test microorganisms were detected in root canals by using dna probes. scanning electron microscopy showed the presence of biofilms in root canals before therapy. root canal systems were incubate ... | 2008 | 18498901 |
| the anti-endotoxic effects of the ksl-w decapeptide on escherichia coli o55:b5 and various oral lipopolysaccharides. | host responses following the recognition of bacterial lipopolysaccharide can range from acute inflammation to septic shock. the aim of this study was to evaluate the ability of the ksl-w decapeptide to bind to and block the endotoxic effects of lipopolysaccharide. | 2008 | 18503516 |
| [periodontitis and cardiovascular disease]. | the prevalence of periodontitis and cardiovascular disease (cvd) is high. a mixed infectious biofilm etiology of periodontitis is known but not fully established in cvd. cofactors; smoking habits, stress, ethnicity, genetics, socioeconomics and age contribute to both diseases. the objectives of this report are to summarize factors in regards to cvd and periodontitis that are clinically relevant. the hypothesis behind a relationship between the two conditions can be founded in (i) shared infectio ... | 2008 | 18517068 |
| in vitro evaluation of the effect of nicotine, cotinine, and caffeine on oral microorganisms. | the aim of this in vitro study was to evaluate the effects of nicotine, cotinine, and caffeine on the viability of some oral bacterial species. it also evaluated the ability of these bacteria to metabolize those substances. single-species biofilms of streptococcus gordonii, porphyromonas gingivalis, or fusobacterium nucleatum and dual-species biofilms of s. gordonii -- f. nucleatum and f. nucleatum -- p. gingivalis were grown on hydroxyapatite discs. seven species were studied as planktonic cell ... | 2008 | 18535638 |
| pcr-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth. | the aim of the present study was to evaluate the presence of the selected pathogens in samples from deciduous and permanent tooth root canals by using pcr method and to determine the association of these organisms with clinical symptoms. | 2008 | 18547832 |
| coaggregation between and among human intestinal and oral bacteria. | coaggregation is believed to facilitate the integration of new bacterial species into polymicrobial communities. the aim of this study was to investigate coaggregation between and among human oral and enteric bacteria. stationary phase cultures of 10 oral and 10 enteric species, chosen on the basis of numerical and ecological significance in their respective environments together with their ease of cultivation, were tested using a quantitative spectrophotometric coaggregation assay in all possib ... | 2008 | 18554305 |
| experimental evidence supports the abscess theory of development of radicular cysts. | the objective of this study was to experimentally induce inflammatory cysts in an animal model so as to test the hypothesis that radicular cysts develop via the "abscess pathway." | 2008 | 18554947 |
| [relationship between herpesviruses and periodontal pathogenic bacteria in subgingival plaque]. | to evaluate the prevalence rates of human cytomegalovirus(hcmv) and epstein-barr virus-1(ebv-1) in subgingival plaque and analyze the relationship between herpesviruses, periodontal pathogenic bacteria and periodontal clinical parameters in chinese patients with aggressive periodontitis(agp). | 2008 | 18560463 |
| rapid and simple detection of eight major periodontal pathogens by the loop-mediated isothermal amplification method. | loop-mediated isothermal amplification (lamp) was applied to develop a rapid and simple detection system for eight periodontal pathogens: aggregatibacter (actinobacillus) actinomycetemcomitans, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, treponema denticola and tannerella forsythia. primers were designed from the 16s ribosomal rna gene for each pathogen, and the lamp amplified the targets specifically and efficiently under ... | 2008 | 18565108 |
| differential gender effects of a reduced-calorie diet on systemic inflammatory and immune parameters in nonhuman primates. | dietary manipulation, including caloric restriction, has been shown to impact host response capabilities significantly, particularly in association with aging. this investigation compared systemic inflammatory and immune-response molecules in rhesus monkeys (macaca mulatta). | 2008 | 18565132 |