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microbiologic diagnostics at titanium implants.the microbiota found at periimplant lesions have been shown to contain putative periodontal pathogens as well as opportunistic species such as staphylococcus spp, enterics, and candida spp. therefore, a microbiologic diagnosis may be of value as guidance before treatment of such lesions.200315127993
the role of lipopolysaccharide in infectious bone resorption of periapical lesion.the role of lipopolysaccharide (lps) in periapical lesion-induced bone resorption was investigated. polymyxin b (pmb), a specific inhibitor of lps, was evaluated to treat the apical lesion.200415128058
surveillance of susceptibility patterns in 1297 european and us anaerobic and capnophilic isolates to co-amoxiclav and five other antimicrobial agents.in vitro susceptibility data were collected for co-amoxiclav and other antimicrobial agents against 1297 recent anaerobe isolates collected in europe and the usa. the co-amoxiclav (amoxicillin/clavulanic acid) mic(50/90)s (amoxicillin/clavulanic acid concentration in a ratio of 2:1, expressed in terms of amoxicillin concentration in mg/l) were 0.5/4 for bacteroides fragilis, </=0.125/1 for prevotella species, </=0.125/0.25 for fusobacterium nucleatum, 0.5/1 for eikenella corrodens, 0.25/8 for pe ...200415128729
another good reason to floss. 200415141231
clonal persistence of oral fusobacterium nucleatum in infancy.once established, early-colonizing bacterial species tend to persist in the mouth. to obtain detailed information on the population dynamics of early-colonizing oral anaerobes, we examined the clonal diversity and persistence of clones among oral fusobacterium nucleatum populations during the first 2 yrs of life. consecutive salivary samples from 12 infants, collected at 2, 6, 12, 18, and 24 mos of age, yielded a total of 546 f. nucleatum isolates for clonal typing with arbitrarily primed pcr (a ...200415153460
apical periodontitis development and bacterial response to endodontic treatment. experimental root canal infections in monkeys with selected bacterial strains.in six monkeys, 160 root canals were inoculated with a combination of four bacterial strains belonging to species streptococcus milleri, peptostreptococcus anaerobius, prevotella oralis, and fusobacterium nucleatum. in two other monkeys, 24 root canals were inoculated with a five-strain combination consisting of these strains and a strain of enterococcus faecalis. all strains were previously isolated from an infected monkey root canal. after 8-12 months, survival of the strains was recorded bact ...200415154917
perforation repair comparing two types of mineral trioxide aggregate.the purpose of this study was to evaluate the ability of two types of mineral trioxide aggregate (mta) to seal furcal perforations in extracted human molars using an anaerobic bacterial leakage model. forty, human, maxillary and mandibular molars were randomly divided into two experimental groups of 18, with two teeth used as positive controls and two teeth without perforations used as negative controls. experimental group 1 was repaired with gray-colored mta and group 2 with off-white-colored m ...200415167471
the effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored proroot mineral trioxide aggregate.the purpose of this in vitro study was to determine whether the substitution of 0.12% chlorhexidine gluconate for sterile water as a mixing agent would enhance the antimicrobial activity of tooth-colored proroot mineral trioxide aggregate (mta) against actinomyces odontolyticus (atcc17982), fusobacterium nucleatum (atcc2586), streptococcus sanguis (atcc10556), enterococcus faecalis (er3/2s), escherichia coli (sm10lambdapir), staphylococcus aureus (atcc6538), pseudomonas aeruginosa (ume), and can ...200415167473
modulation of the antibody response by porphyromonas gingivalis and fusobacterium nucleatum in a mouse model.successive immunization of mice with fusobacterium nucleatum and porphyromonas gingivalis has been shown to modulate the specific serum igg responses to these organisms. the aim of this study was to investigate these antibody responses further by examining the igg subclasses induced as well as the opsonizing properties of the specific antibodies. serum samples from balb/c mice immunized with f. nucleatum (gp1-f), p. gingivalis (gp2-p), p. gingivalis followed by f. nucleatum (gp3-pf) f. nucleatum ...200415209995
effect of supragingival plaque control on subgingival microflora and gingivo-periodontal tissues.the aim of this study was to carry out a short term evaluation of the effect of supragingival plaque control on the subgingival microflora and gingivoperiodontal tissues in patients with moderate and severe periodontitis. a total of 92 sites from 23 patients were studied; patients ranged between 35 and 60 years of age (x 46.4 +/- 1.71). plaque index (pi), gingival index (gi), bleeding on probing (bp), probing depth (pd), and attachment level (al) were measured. subgingival microflora of the same ...200015211927
subgingival microbiota of chilean patients with chronic periodontitis.an association between race/ethnicity and the composition of the subgingival microbiota has been found in chronic periodontitis. a study was undertaken to determine the characteristics of the subgingival microbiota of chronic periodontitis in chileans residing in santiago.200415212354
comparison of systemic flurithromycin therapy and clinical procedures in the treatment of periodontal diseases.the purpose of the present investigation was to evaluate, in 20 periodontal patients, the microbial and clinical effects of flurithromycin therapy plus scaling and root planning (srp) in comparison with srp alone. clinical assessments of plaque, bleeding on probing and pocket depth were made prior to srp alone and srp plus flurithromycin therapy (375 mg twice daily for 5 days) and after both treatments. subgingival plaque samples (n. 180) were taken prior to and after both treatments and analyze ...200415216949
evaluation of the whole genome dna-dna hybridization technique to identify bacteria in histological sections of periradicular lesions.whole genome dna-dna hybridization has been used to identify bacteria in periradicular lesions partly because there is no amplification of the bacteria, therefore, minor contaminants are not detected. there are, however, potential pitfalls with this technique, including inability to distinguish dead bacteria, cross-reactions of species within a genus, and inability to detect species present in low numbers because of loss of dna during extraction and purification. alternatively, inadequate extrac ...200415220650
portal vein thrombosis associated with fusobacterium nucleatum septicemia in a patient with ulcerative colitis. 200415232367
development of a multispecies oral bacterial community in a saliva-conditioned flow cell.microbial communities within the human oral cavity are dynamic associations of more than 500 bacterial species that form biofilms on the soft and hard tissues of the mouth. understanding the development and spatial organization of oral biofilms has been facilitated by the use of in vitro models. we used a saliva-conditioned flow cell, with saliva as the sole nutritional source, as a model to examine the development of multispecies biofilm communities from an inoculum containing the coaggregation ...200415240317
active domains of salivary statherin on apatitic surfaces for binding to fusobacterium nucleatum cells.fusobacterium nucleatum can bind to saliva-coated tooth surfaces. however, the nature of the domains of salivary protein that interact with f. nucleatum remains unclear. the ability of individual proteins in human submandibular-sublingual saliva (hsmsl) to bind f. nucleatum cells was examined by dot blot assay; statherin displayed the strongest binding activity. statherin binding sites were determined based on binding of (125)i-labelled f. nucleatum to statherin-coated hydroxyapatite (shap) bead ...200415256578
[purification and characterization of sanguicin--a bacteriocin produced by streptococcus sanguis].streptococcus sanguis plays an important role in maintaining the periodontal microecological balance. previous studies have demonstrated that sanguicin--a kind of bacteriocin produced by s. sanguis has a prominent function of inhibiting the growth of putative periodontopathic bacteria (ppb). the aim of this study was to purify sanguicin and investigate its characters.200415291127
implant therapy following liver transplantation: clinical and microbiological results after 10 years.the number of immunocompromised patients is increasing as a consequence of successful organ transplantation. placing dental implants in these patients has been questioned because of their increased risk for infections. the 10-year follow-up data of a 71-year-old liver transplant recipient with long-term immunosuppressive therapy is reported. six months after liver transplantation, two interforaminal implants were inserted in the edentulous mandible, and an overdenture using non-rigid telescopic ...200415295960
identification and sequencing of bacterial rdnas in culture-negative amniotic fluid from women in premature labor.our objective was to identify bacterial species present in culture-negative but 16s rdna-positive amniotic fluid samples from women in preterm labor. amniotic fluid from 69 women in preterm labor was cultured and examined for the proinflammatory cytokine interleukin-6 (il-6). polymerase chain reaction technology was used to detect highly conserved bacterial ribosomal dna sequences (16s rdnas). as previously reported, 16s rdnas were identified in 15 (94%) of 16 culture-positive amniotic fluid sam ...200415311367
the participation of nitric oxide in peritoneal exudate cell cytotoxicity of mice by fusobacterium nucleatum.previously we reported that mice infected recurrently with live fusobacterium nucleatum(fn) synthesize a significant amount of no between 12 hr and 24 hr after fn injection. fn is a gram-negative rod periodontal pathogen. no could not be induced by heat-killed fn or in untreated mice. this no, derived from the inos after infection of live fn, was not involved in the fn reduction because fn clearance occurs within 6 hr. we investigated in this study whether this no was involved in cytotoxicity in ...200415322341
beta-lactamase production and antimicrobial susceptibility of subgingival bacteria from refractory periodontitis.this study assessed the extent of beta-lactamase-producing bacteria in subgingival plaque samples obtained from 25 patients with refractory marginal periodontitis in the usa. beta-lactamase-positive isolates were characterized using commercial diagnostic kits and partial sequencing of the 16s rrna gene. the susceptibilities to different antimicrobial agents were tested and, in addition, the isolates were screened for the presence of extended spectrum beta-lactamases (esbls). beta-lactamase-produ ...200415327642
selected endodontic pathogens in the apical third of infected root canals: a molecular investigation.bacteria located at the apical portion of the root canals are conceivably in a strategic position to induce damage to the periradicular tissues and resulting inflammatory diseases. this study sought to investigate the prevalence of 11 selected putative endodontic pathogens in the apical third of infected root canals associated with periradicular lesions. the apical root portion of 23 extracted teeth with carious pulpal exposures and attached periradicular lesions was sectioned, and the root cana ...200415329567
clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis.the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy.200415367191
fusobacterium canifelinum sp. nov., from the oral cavity of cats and dogs.fourteen strains of gram-negative, anaerobic, fluoroquinolone-resistant, non-sporulating rods were isolated from various infections in cats and dogs, as well as from wounds in humans after cat- or dog-bites. these strains were characterized by sequencing of the 16s-23s rdna internal transcribed spacer (its) regions, 16s rdna, dna-dna hybridization, phylogenetic analysis, and phenotypic tests. the results indicate that the novel strains belong to a distinct species, closely related to fusobacteri ...200415368845
mesenteric adenitis and portal vein thrombosis due to fusobacterium nucleatum.we report the first description of portal and mesenteric vein thrombosis associated with suppurative mesenteric adenitis in a 71-year-old woman. the bacterium detected in mesenteric lymph nodes was fusobacterium nucleatum, an anaerobic gram-negative bacillus. our patient had a clinical syndrome of pharyngitis and fever preceding portal vein thrombosis. abdominal symptoms improved with antibiotics and anticoagulant therapy. this location of f. nucleatum in mesenteric lymph nodes provides an inter ...200415371934
dehydration of (r)-2-hydroxyacyl-coa to enoyl-coa in the fermentation of alpha-amino acids by anaerobic bacteria.several clostridia and fusobacteria ferment alpha-amino acids via (r)-2-hydroxyacyl-coa, which is dehydrated to enoyl-coa by syn-elimination. this reaction is of great mechanistic interest, since the beta-hydrogen, to be eliminated as proton, is not activated (pk 40-50). a mechanism has been proposed, in which one high-energy electron acts as cofactor and transiently reduces the electrophilic thiol ester carbonyl to a nucleophilic ketyl radical anion. the 2-hydroxyacyl-coa dehydratases are two-c ...200415374661
in situ biofilm formation by multi-species oral bacteria under flowing and anaerobic conditions.an understanding of biofilm behavior of periodontopathic bacteria is key to the development of effective oral therapies. we hypothesized that interspecies bacterial aggregates play an important role in anaerobic biofilm establishment and proliferation, and contribute to the survivability of the biofilm against therapeutic agents. the system developed in this study assessed a multi-species (streptococcus gordonii, actinobacillus actinomycetemcomitans, and fusobacterium nucleatum) biofilm formatio ...200415381723
binding of pro-matrix metalloproteinase 9 by fusobacterium nucleatum subsp. nucleatum as a mechanism to promote the invasion of a reconstituted basement membrane.in this study, we investigated the ability of fusobacterium nucleatum subsp. nucleatum to increase its tissue-invasive potential by acquiring cell-associated human matrix metalloproteinase 9 (mmp-9) activity. binding of pro-mmp-9 to fusobacteria was demonstrated by enzyme-linked immunosorbent assay. zymography and a colorimetric assay showed that bound pro-mmp-9 can be converted into a proteolytically active form. the potential contribution of this acquired host activity in tissue invasion was d ...200415385524
cavernous sinus thrombosis and cerebral infarction caused by fusobacterium nucleatum infection.we report an unusual case of fusobacterial infection with secondary intracranial invasion. the condition was complicated by a cavernous sinus thrombosis and ischemic stroke. the patient was a 63-year-old woman with no history of systemic disease who had undergone a tooth extraction before the onset of symptoms. she initially suffered from sphenomaxillary sinusitis and a cavernous sinus thrombosis, and subsequently developed meningitis. cerebrospinal fluid examination suggested a pyogenic infecti ...200415455548
periodontal pathogens in atheromatous plaques. a controlled clinical and laboratory trial.a possible relationship between periodontitis and cardiovascular disease has been suggested. the aims of this controlled clinical study were: (i) to ascertain the presence of periodontal bacteria dna [actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis (formerly bacteroides forsythus)] in carotid atheromatous plaques and (ii) to assess the concomitant presence of the same periodontal bacteria dna, if any, in peri ...200415491349
alkali-resistant bacteria in root canal systems.the aim of this study was to isolate and identify alkali-resistant bacteria from the dentin of infected root canals. bacteria from homogenized dentin powder made up from infected root canal walls from human teeth were cultured on buffer-enriched brain heart infusion agar supplemented with 4% sheep blood (bhi-blood agar), adjusted to ph 7.0, 9.0 or 10.0. incubation took place for 7 days at 37 degrees c in an anaerobic glove box. bacterial strains selected according to colony and morphology were s ...200415491465
microbiological evaluation of endodontic files after cleaning and steam sterilization procedures.infection control procedures are essential for modern dental practice and they are continually evolving to meet the dental profession's high standards. the present study evaluated the efficacy of two cleaning procedures to reduce bacterial numbers on endodontic files, and evaluated the effect of biological debris on the subsequent sterilization of files.200415497355
antiproliferative activity of actinobacillus (haemophilus) actinomycetemcomitans and fusobacterium nucleatum in peripheral blood mononuclear cells.several studies indicate actinobacillus (haemophilus) actinomycetemcomitans and fusobacterium nucleatum as etiologic agents of periodontal disease. immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. this study evaluated the antiproliferative activity of ammonium precipitate fractions of a. (h.) actinomycetemcomitans and f. nucleatum isolates from humans and marmosets both with and without periodontal disease. all a. (h.) acti ...200415501650
rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis.although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ...200415508748
phylogenetic analysis of fusobacterium necrophorum, fusobacterium varium and fusobacterium nucleatum based on gyrb gene sequences.the nucleotide sequences of the dna gyrase b subunit gene (gyrb) of fusobacterium necrophorum subsp. necrophorum, f. necrophorum subsp. funduliforme and f. varium were determined and analyzed together with those of f. nucleatum subsp. nucleatum and f. nucleatum subsp. vincentii. on the phylogenetic tree constructed, the strains of each fusobacterial species formed distinct clusters with deep sublines. the degree of sequence similarity within each cluster was 93.2% or more, whereas similarities b ...200415528856
investigation of the effect of the coronal restoration quality on the composition of the root canal microflora in teeth with apical periodontitis by means of t-rflp analysis.to investigate the effect of the radiographic and clinical quality of coronal restorations on the composition of the root canal flora of teeth with necrotic pulps and teeth with root fillings associated with apical periodontitis.200415548272
[an unusual case of hepatic abscess].right upper quadrant pain with chronic malaise and fever can be the clinical manifestation of a hepatic infection. an uncommon cause for the disease is hepatic actinomycosis. actinomycosis was common in the preantibiotic era but is less frequent nowadays; consequently its timely recognition has become more difficult. the clinical and radiological findings often resemble other inflammatory and neoplastic lesions. we report a case of a mixed anaerobic liver abscess including fusobacteria and actin ...200415553899
["in vitro" activity of ten antimicrobial agents against anaerobic bacteria. a collaborative study, 1999-2002].the antimicrobial activity of ampicillin, ampicillin-sulbactam, cefoxitin, ceftriaxone, imipenem, piperacillin, piperacillin-tazobactam, clindamycin, metronidazole, and azitromycin was assesed against 166 strains of anaerobic bacteria recovered from eight hospitals in buenos aires. the strains studied were bacteroides fragilis group (65), fusobacterium spp. (26), prevotella spp. (21), porphyromonas spp. (10), clostridium difficile (10), other clostridia (12), and gram-positive cocci (22). the mi ...200415559195
single species biofilm-forming ability of root canal isolates on gutta-percha points.the participation of bacterial biofilms in the over-filled gutta-percha points associated with refractory periapical periodontitis has recently been reported. this study investigated the initial biofilm-forming ability of root canal isolates (enterococcus faecalis, streptococcus sanguis, strep. intermedius, strep. pyogenes, staphylococcus aureus, fusobacterium nucleatum, propionibacterium acnes, porphyromonas gingivalis and prevotella intermedia) on gutta-percha points in vitro. each bacterial s ...200415560836
killing of periodontopathogenic bacteria by photodynamic therapy.the aim of this study was to evaluate a new approach for killing periodontopathogenic bacteria using photodynamic therapy (pdt).200415562911
evolutionary relationships of fusobacterium nucleatum based on phylogenetic analysis and comparative genomics.the phylogenetic position and evolutionary relationships of fusobacteria remain uncertain. especially intriguing is their relatedness to low g+c gram positive bacteria (firmicutes) by ribosomal molecular phylogenies, but their possession of a typical gram negative outer membrane. taking advantage of the recent completion of the fusobacterium nucleatum genome sequence we have examined the evolutionary relationships of fusobacterium genes by phylogenetic analysis and comparative genomics tools.200415566569
characterization of the novel fusobacterium nucleatum plasmid pkh9 and evidence of an addiction system.fusobacterium nucleatum is an important oral anaerobic pathogen involved in periodontal and systemic infections. studies of the molecular mechanisms involved in fusobacterial virulence and adhesion have been limited by lack of systems for efficient genetic manipulation. plasmids were isolated from eight strains of f. nucleatum. the smallest plasmid, pkh9 (4,975 bp), was characterized and used to create new vectors for fusobacterial genetic manipulation. dna sequence analysis of pkh9 revealed an ...200415574887
periimplantitis.orodental rehabilitation through the use of implants offers very high success rates. in this paper, we describe some of the complications involved with this technique, such as periimplant disease and, within this category, periimplantitis, an inflammatory reaction in which there is a loss of the bony support of the implant accompanied by inflammation. the aetiology of the disease is conditioned by the status of the tissue surrounding the implant, implant design, degree of roughness, the poor ali ...200415580138
the aetiology and treatment of oral halitosis: an update.halitosis refers to the condition of offensive mouth odour. more than 90% of cases of halitosis originate from the oral cavity. the implicated bacteria (fusobacterium nucleatum, prevotella intermedia, and tannerella forsythensis) are located in stagnant areas in the oral cavity, such as the dorsal surface of tongue, periodontal pockets, and interproximal areas. these bacteria proteolyse the amino acids releasing volatile sulphur compounds. the management of halitosis involves determining and eli ...200415591601
coaggregation profiles of the microflora from root surface caries lesions.bacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. as no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single candida spp. the quantitative coa ...200515598414
synergy between tannerella forsythia and fusobacterium nucleatum in biofilm formation.during dental plaque formation, the interaction of different organisms is important in the development of complex communities. fusobacterium nucleatum is considered a 'bridge-organism' that facilitates colonization of other bacteria by coaggregation-mediated mechanisms and possibly by making the environment conducive for oxygen intolerant anaerobes. these studies were carried out to determine whether coaggregation between f. nucleatum and tannerella forsythia is important in the formation of mix ...200515612944
inhibitory effects of various micro-organisms on the growth of helicobacter pylori.to examine the in vitro influence of various bacteria species on helicobacter pylori (hp) growth.200515613007
differential regulation of cytokine genes in gingival epithelial cells challenged by fusobacterium nucleatum and porphyromonas gingivalis.il-8 mrna in human gingival epithelial cells (hgecs) is up-regulated by fusobacterium nucleatum, and up-/down-regulated by porphyromonas gingivalis in a complex interaction in the early stages (< or = 4 h) after infection. the mechanisms involved in this regulation in response to f. nucleatum and/or p. gingivalis infection, and identification of co-regulated cytokine genes, are the focus of this investigation. heat, formalin or protease treatment of f. nucleatum cells attenuated the il-8 mrna up ...200415619426
attachment of oral gram-negative anaerobic rods to a smooth titanium surface: an electron microscopy study.attachment of bacteria to titanium may differ not only between bacterial species but also between strains within a species. the aim of the present in vitro study was to examine differences in bacterial attachment using 4 gram-negative anaerobic species of bacteria that are considered potential periodontal pathogens.200415623054
phototoxic effect of visible light on porphyromonas gingivalis and fusobacterium nucleatum: an in vitro study.the antibacterial effect of visible light irradiation combined with photosensitizers has been reported. the objective of this was to test the effect of visible light irradiation without photosensitizers on the viability of oral microorganisms. strains of porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mutans and streptococcus faecalis in suspension or grown on agar were exposed to visible light at wavelengths of 400-500 nm. these wavelengths are used to photopolymerize composite ...200415623322
the role of anaerobic bacteria in tonsillitis.this review summarizes the information that supports the potential importance of anaerobic bacteria in tonsillitis. some anaerobic bacteria possess interfering capability with group a beta-hemolytic streptococci (gabhs) and other pathogens. the possible role of anaerobes in the acute inflammatory process in the tonsils is supported by several observations: anaerobes have been isolated from the cores of tonsils of patients with recurrent gabhs and non-gabhs tonsillitis (nst); the recovery of anae ...200515627441
effectiveness of a triclosan/copolymer dentifrice on microbiological and inflammatory parameters.according to the us surgeon general's report, "oral health in america," published in 2000, most adults in the united states show some degree of periodontal pathology, with severe periodontal diseases affecting about 14% of middle-aged adults. periodontal diseases are polymicrobial-induced inflammatory diseases, and they vary from mild gingival inflammation to severe deterioration of the periodontium, ie, loss of periodontal supportive tissues and, ultimately, tooth loss. new evidence shows that ...200415645886
fusobacterium nucleatum increases collagenase 3 production and migration of epithelial cells.fusobacterium nucleatum is closely associated with human periodontal diseases and may also be a causative agent in other infections, such as pericarditis, septic arthritis, and abscesses of tonsils and liver. initiation and outcome of infective diseases depend critically on the host cell signaling system altered by the microbe. production of proteinases by infected cells is an important factor in pericellular tissue destruction and cell migration. we studied binding of f. nucleatum to human epit ...200515664960
periodontal infections and pre-term low birth weight: a case-control study.pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ...200515691348
dna-dna hybridization demonstrates multiple bacteria in osteoradionecrosis.bone necrosis secondary to radiation was previously attributed to trauma of devitalized bone and microbiological sepsis. however, conventional microbiological technique has failed to demonstrate microorganisms throughout osteoradionecrotic bone, claimed to be hypoxic, hypovascular and hypocellular. the aim of the present study was to examine such bone for bacteria using dna-dna hybridization. compared to standard culture methods this technique enables the investigation of a vast number of bacter ...200515695050
molecular analysis of microbial diversity in advanced caries.real-time pcr analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. this suggests that an unresolved complexity exists in bacteria associated with advanced caries. in this report, the profile of the microflora of carious dentine was explored by using dna extracted from 10 lesions selected on the basis of comparable total microbial load and on the relative abundance of prevotella spp. using universal primers for t ...200515695690
development and characterization of a simple perfused oral microcosm.to validate perfused, inline, filter-based fermentation systems (multiple sorbarod devices, msd) for their ability to maintain stable oral bacterial communities. msd enable replicate (n=5) microcosm biofilms (bf) to be established and sampled, together with their perfusates (pa, cells in eluted medium).200515715865
antimicrobial actions of benzimidazoles against oral streptococci.benzimidazoles, such as lansoprazole and omeprazole, are used extensively as proton-pump inhibitors to control stomach acid secretion and also have antimicrobial actions against helicobacter pylori. our objective was to determine whether they are potentially useful antimicrobials against oral bacteria.200515720569
impact of sample storage on detection of periodontal bacteria.information on the impact of sample storage prior to analysis by dna methods is limited. the aim of this study was to investigate the effect of subgingival sample storage on bacterial detection and enumeration.200515720575
thiazolidinedione (pioglitazone) blocks p. gingivalis- and f. nucleatum, but not e. coli, lipopolysaccharide (lps)-induced interleukin-6 (il-6) production in adipocytes.an elevated level of c-reactive protein (crp) predicts the future development of coronary heart disease. periodontitis appears to up-regulate crp. crp is produced by hepatocytes in response to interleukin-6 (il-6). a major source of il-6 in obese subjects is adipocytes. we hypothesized that lipopolysaccharide (lps) from periodontal pathogens stimulated adipocytes to produce il-6, and that the production was suppressed by the drugs targeted against insulin resistance, thiazolidinedione (pioglitaz ...200515723863
quantitative detection of volatile sulfur compound- producing microorganisms in oral specimens using real-time pcr.it is well-known that some periodontopathic bacteria, especially porphyromonas gingivalis, fusobacterium nucleatum, tannerella forsythia (formerly bacteroides forsythus or tan. forsythensis), and treponema denticola, actively produce volatile sulfur compounds (vscs), such as h2s and ch3sh. we previously reported a qualitative relationship between periodontopathic bacteria and vscs; however, a quantitative analysis of periodontopathic bacteria in oral specimens is required for further characteriz ...200515752104
[pharmacokinetic/pharmacodynamic analysis of antibiotic therapy in dentistry and stomatology].this study evaluates the efficacy of various antimicrobial treatments for orofacial infections on the basis of pharmacokinetic/pharmacodynamic (pk/pd) criteria.200515757581
bacteria recovered from dental pulp induce apoptosis of lymph node cells.apoptosis is critical in the pathogenesis of several infectious diseases. the induction of apoptosis was assessed in mouse lymph node cells by four bacteria recovered from infected human dental pulp: gemella morbillorum, clostridium butyricum, fusobacterium nucleatum and bifidobacterium adolescentis. smaller lymph nodes and smaller numbers of cells were observed after experimental dental pulp infection with c. butyricum, suggesting that this bacterium induces cell death. apoptosis was evaluated ...200515770029
portal vein thrombosis despite anticoagulation in a person with diabetes. 200515805557
towards a resolution on the inherent methodological weakness of the "effective number of codons used by a gene".recently anders fuglsang provided a modified way for calculating n(c) when biased discrepancy is present in a gene [biochem. biophys. res. commun. 317 (2004) 957]. instead of taking the average codon homozygosity for each synonymous family type (as proposed by wright) [gene 87 (1990) 23] fuglsang considered codon homozygosity of each amino acid individually. marsashi and najafabadi [biochem. biophys. res. commun. 324 (2004) 1] in their recent article demonstrated that the readjustment for overes ...200515823544
differences in prevalence of selected bacterial species in primary endodontic infections from two distinct geographic locations.this study intended to compare the prevalence of 7 putative endodontic pathogens in samples of primary endodontic infections taken from patients of 2 distant geographic locations.200515829891
dna probe identification of bacteria colonizing internal surfaces of the implant-abutment interface: a preliminary study.currently, there is limited knowledge concerning the specific genus and species of bacteria that may colonize internal surfaces of the implant-abutment interface (iai) of two-stage dental implants. the purpose of this study was to use dna probe analysis to identify those periodontopathic bacteria that may inhabit the internal surfaces and healing abutment screw-threads of the iai of dental implants in situ.200515830645
association of a novel high molecular weight, serine-rich protein (srpa) with fibril-mediated adhesion of the oral biofilm bacterium streptococcus cristatus.the surface of the oral plaque bacterium streptococcus cristatus is decorated with a lateral tuft of fibrils. the fibrillar tuft functions in the adhesion of s. cristatus to heterologous bacterial species in the plaque biofilm. the tuft typically consists of a densely packed fringe of shorter fibrils 238 +/- 19 nm long with longer, less abundant fibrils 403 +/- 66 nm long projecting through the fringe of short fibrils. the two types of fibrils in the tufts of s. cristatus have been refractory to ...200515836513
effect of khat chewing on 14 selected periodontal bacteria in sub- and supragingival plaque of a young male population.the habit of chewing khat (catha edulis) for its amphetamine-like effects is highly prevalent in yemen and east africa, and has expanded to western countries. the purpose of this study was to estimate and compare the prevalence and levels of 14 periodontal bacteria in gingival plaque of khat chewers and khat nonchewers, as well as of khat chewing sides and khat nonchewing sides.200515836514
detection of heat shock proteins but not superantigen by isolated oral bacteria from patients with behcet's disease.we isolated bacteria from periodontal sites and mixed saliva in eight patients with behcet's disease and surveyed them to determine whether they produced heat shock proteins (hsps) and superantigen. cultivable bacterial compositions from periodontal sites and saliva were examined by anaerobic culture using blood agar plates. gram-negative anaerobic rods such as prevotella intermedia, fusobacterium nucleatum, and capnocytophaga species were predominant in the isolates from the subgingival plaque ...200515836518
treponema medium glycoconjugate inhibits activation of human gingival fibroblasts stimulated with phenol-water extracts of periodontopathic bacteria.oral treponemes are well-known as causative agents of periodontal diseases; however, the details have not been fully clarified. here, we examined the effects of treponema medium glycoconjugate on the activation of human gingival fibroblasts using phenol-water extracts from porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum subsp. nucleatum, and actinobacillus actinomycetemcomitans. the phenol-water extracts activated human gingival fibroblasts to mediate il-8 production, as ...200515840783
oral microbiology and genomics. 200515853937
failed dental implants - clinical, radiological and bacteriological findings in 17 patients.the aim of this study was to evaluate the reasons for implant failure in two oral and maxillofacial units in helsinki. seventeen patients, who lost 30 implants were included in this study.200515878525
tobacco smoking and periodontal microflora in a saudi arabian population.to explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits.200515882210
susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, {beta}-defensins and ll37, produced by human epithelial cells.antimicrobial peptides are one of the factors involved in innate immunity. the susceptibility of periodontopathogenic and cariogenic bacteria to the major antimicrobial peptides produced by epithelia was investigated.200515886266
microbial changes in conjunctival flora with 30-day continuous-wear silicone hydrogel contact lenses.to determine the effect of 30-day continuous-wear silicone hydrogel contact lenses on the conjunctival flora in asymptomatic wearers.200515894879
identification of cultivable microorganisms from primary endodontic infections with exposed and unexposed pulp space.this study was aimed at comparing the cultivable microorganisms in canals with periapical radiolucencies with exposed and unexposed pulp space. microbiological samples were taken and analyzed from 45 canals with exposed pulp space, and 43 canals with unexposed pulp space. the canal contents were analyzed by aerobic/anaerobic culture, and conventional identification techniques. there were 211 isolates of bacteria belonging to 28 genera and 55 species recovered from exposed canals. in the unexpose ...200515917680
molecular identification of an invasive gingival bacterial community.a woman with neutropenia developed gingival hyperplasia. biopsy showed invasion of gingival tissue with mats of filamentous organisms, and molecular analysis by polymerase chain reaction and fluorescence in situ hybridization revealed capnocytophaga sputigena, leptotrichia species, and fusobacterium nucleatum. oral bacterial flora may cause invasive gingival disease with hyperplasia in immunocompromised patients.200515937752
antimicrobial susceptibility variation of 50 anaerobic periopathogens in aggressive periodontitis: an interindividual variability study.the frequent use of antibiotics in developed countries has led to the emergence of widespread bacterial resistance. in this study, the interindividual variability of the antibiotic susceptibility of 50 putative microorganisms in aggressive periodontitis patients has been evaluated by means of vc (variation coefficient).200515943770
microarrays complement culture methods for identification of bacteria in endodontic infections.the aim of this study was to investigate the microbial composition of necrotic root canals using culture methods and microarray technology. twenty uniradicular teeth with radiographic evidence of periapical bone loss and with no previous endodontic treatment were selected for this study. for molecular diagnosis a dna chip with 20 different species-specific, 16s-rdna-directed catcher probes was used. the microorganisms most frequently detected by the dna chip were: micromonas micros, fusobacteriu ...200515943771
fiber optic fluorescence microprobe for endodontic diagnosis.successful endodontic therapy requires total debridement as well as complete obturation of the root canal to the cemento-dentinal junction. the goal of this study was to investigate the feasibility of using quantitative fluorescence spectroscopy for the detection and localization of pathological dentin, pulpal remnants, and microorganisms within the root canal. specific aims were to identify: 1) characteristic excitation/emission spectra for healthy dentin, decayed dentin, enamel, and pulp; 2) t ...200515947209
multi-target antimicrobial actions of zinc against oral anaerobes.zinc is used in oral care products as an antiplaque/antigingivitis agent. our objective was to assess the antimicrobial actions of zinc against oral anaerobes associated with gingivitis, specifically fusobacterium nucleatum and prevotella intermedia, with focus on catabolism and oxidative metabolism.200515958206
mechanism of visible light phototoxicity on porphyromonas gingivalis and fusobacterium nucleatum.phototoxicity of visible light laser on the porphyrin-producing bacteria, porphyromonas gingivalis, in the absence of photosensitizers and under aerobic conditions was shown in previous studies. recently, we found that the noncoherent visible light sources at wavelengths of 400-500 nm, commonly used in restorative dentistry, induced a phototoxic effect on p. gingivalis, as well as on fusobacterium nucleatum, and to a lesser extent on the streptococci sp. to elucidate the mechanism of this photot ...200515960594
comparison of culture and real-time pcr for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples.bacterial cultivation is a well-established method for analyzing plaque samples. real-time polymerase chain reaction (pcr) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. in this study, we compared real-time pcr with conventional anaerobic cultivation.200515966886
prevalence of periodontopathic bacteria in aggressive periodontitis patients in a chilean population.actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis (agp). other periodontopathic bacteria such as porphyromonas gingivalis are also suspected of participating in aggressive periodontitis although the evidence to support this is controversial. the aim of the present study was to determine the prevalence of eight periodontopathic bacteria in chilean patients with agp.200515974855
fluorescence based measurements of fusobacterium nucleatum coaggregation and of fusobacterial attachment to mammalian cells.fusobacterium nucleatum is a common oral anaerobe associated with gingivitis, periodontal disease and preterm deliveries. coaggregation among oral bacteria is considered to be a significant factor in dental plaque development. adhesion to host cells was suggested to be important for the f. nucleatum virulence associated with oral inflammation and with preterm births. an uncharacterized fusobacterial galactose inhibitible adhesin mediates coaggregation of f. nucleatum 12230 and f. nucleatum pk159 ...200515993010
protein secretion systems in fusobacterium nucleatum: genomic identification of type 4 piliation and complete type v pathways brings new insight into mechanisms of pathogenesis.recent genomic analyses of the two sequenced strains f. nucleatum subsp. nucleatum atcc 25586 and f. nucleatum subsp. vincentii atcc 49256 suggested that the major protein secretion systems were absent. however, such a paucity of protein secretion systems is incongruous with f. nucleatum pathogenesis. moreover, the presence of one or more such systems has been described for every other gram-negative organism sequenced to date. in this investigation, the question of protein secretion in f. nuclea ...200515993836
an antibacterial surface on dental implants, based on the photocatalytic bactericidal effect.it is well known that the moderately roughened surfaces of dental implants enhance direct bone-implant contact. however, rough implant surfaces, as compared to smooth surfaces, are thought to pose a higher risk of bacterial infection when exposed to the oral cavity.200515996357
antimicrobial profiles of periodontal pathogens isolated from periodontitis patients in the netherlands and spain.antimicrobial resistance of periodontal pathogens towards currently used antibiotics in periodontics has been investigated in a previous study. microbial resistance in the periodontal microflora was more frequently observed in spanish patients in comparison with dutch patients. the aim of the present study was to compare antimicrobial susceptibility profiles of five periodontal bacteria isolated from periodontitis patients in spain and in the netherlands.200515998275
efficacy of photodynamic therapy on inflammatory signs and two selected periodontopathogenic species in a beagle dog model.current research aims to find alternatives to conventional methods for suppressing periodontopathogenic bacteria. photodynamic therapy (pdt) could be a suitable treatment procedure of periodontal infections.200516018752
identification and characterization of a novel adhesin unique to oral fusobacteria.fusobacterium nucleatum is a gram-negative anaerobe that is prevalent in periodontal disease and infections of different parts of the body. the organism has remarkable adherence properties, binding to partners ranging from eukaryotic and prokaryotic cells to extracellular macromolecules. understanding its adherence is important for understanding the pathogenesis of f. nucleatum. in this study, a novel adhesin, fada (fusobacterium adhesin a), was demonstrated to bind to the surface proteins of th ...200516030227
vertebral osteomyelitis due to fusobacterium species: report of three cases and review of the literature.we describe three cases of fusobacterium spp. diskitis and review with attention to risk factors, clinical features, diagnosis, treatment and outcome. in most of the reported cases, a ear-nose-throat infection was found. clinical manifestations were similar to those of classic bacterial vertebral osteomyelitis. clindamycin is the most appropriate antibiotic. the outcome seems to be very good without relapse with appropriate treatment compared to pyogenic vertebral osteomyelitis.200516038751
fusobacterium nucleatum apoptosis-inducing outer membrane protein.the periodontal pathogen fusobacterium nucleatum induces apoptosis in lymphocytes. we previously identified the autotransporter protein fap2 in f. nucleatum strain pk1594 that induced apoptosis in lymphocytes when expressed in escherichia coli. in this study, we identified protein homologs of fap2 in the transformable f. nucleatum strain atcc 23726, to determine their role in the induction of apoptosis in lymphocytes. we used a new gene-inactivation vector conferring thiamphenicol resistance (ph ...200516040725
[efficacy variation of erythromycin and spiramycin on periopathogens in aggressive periodontitis. an in vitro comparative study].erythromycin (ery) and spiramycin (spi) are the most frequently prescribed macrolides by dentists. however, the emergence of resistant anaerobic subgingival bacteria imposes an increased vigilance. this study aims to compare these macrolides efficacy on principal periopathogens.200516087299
the effects of intra-amniotic injection of periodontopathic lipopolysaccharides in sheep.periodontal disease may cause several complications of pregnancy, including fetal death. the purpose of this study was to investigate in sheep the effects of the intra-amniotic injection of lipopolysaccharide from 3 periodontopathic organisms and to compare these effects with those resulting from similar injection of escherichia coli lipopolysaccharide. the outcomes that were studied included the rates of fetal death and the features of inflammation and lung maturation in survivors.200516098849
differential induction of human beta-defensin expression by periodontal commensals and pathogens in periodontal pocket epithelial cells.to investigate the possible role of beta-defensins in gingival health and periodontal disease, we examined the effect of several stimuli on the expression of interleukin-8 (il-8), human beta-defensin-1, -2, -3, and -4 (hbd) in primary human diseased gingival epithelial (hge) cell cultures from periodontitis patients by quantitative taqman reverse transcription polymerase chain reaction (rt-pcr).200516101361
cross-reactivity of groel antibodies with human heat shock protein 60 and quantification of pathogens in atherosclerosis.chronic infections such as those caused by chlamydia pneumoniae and periodontopathic bacteria such as porphyromonas gingivalis have been associated with atherosclerosis, possibly due to cross-reactivity of the immune response to bacterial groel with human heat shock protein (hhsp) 60.200516101965
efficient gene transfer and targeted mutagenesis in fusobacterium nucleatum.fusobacterium nucleatum is a gram-negative anaerobe important in dental biofilm ecology and infectious diseases with significant societal impact. the lack of efficient genetic systems has hampered molecular analyses in this microorganism. we previously reported construction of a shuttle plasmid, phs17, using the native fusobacterial plasmid pfn1 and an erythromycin resistance cassette. however, the host range of phs17 was restricted to f. nucleatum, atcc 10953, and the transformation efficiency ...200616115683
enhanced pathogenicity of fusobacterium nucleatum adapted to oxidative stress.fusobacterium nucleatum is an obligate anaerobic bacterium found in the indigenous human microbiota but also recovered from several anaerobic infections. considering the biological and medical relevance of f. nucleatum, the characterization of its response to oxidative stress is needed in order to understand how this anaerobic bacterium survives during an invasive process of oxygenated tissues. influence of oxidative stress by atmospheric oxygen exposure on cellular morphology and pathogenicity ...200516125361
antibacterial activity of aqueous extracts of selected chewing sticks.this aim of this study was to determine the antibacterial activity in extracts obtained from various nigerian chewing sticks. aqueous extracts from seventeen chewing sticks and the fruit of c. ferruginea, one fruit used in oral hygiene in nigeria, were screened for antibacterial activity against type cultures of staphylococcus aureus, bacillus subtilis, escherichia coli, and pseudomonas aeruginosa. eleven of the test extracts showed activity against at least two of these referenced organisms. mi ...200516127476
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