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anaerobic bacteria in biliary disease in elderly patients.gallbladder bile from 52 elderly subjects who had undergone biliary tract surgery was examined for the presence of bacteria. twelve patients had sterile bile, 18 specimens of bile yielded anaerobes as well as aerobes, and 22 yielded aerobic bacteria only. escherichia coli was the most commonly isolated organism (30 strains). bacteroides fragilis was the most frequently encountered anaerobic bacterium and was found in 15 patients. the klebsiella-enterobacter group was the second most commonly iso ...1977192815
glucose utilization by resting cells of fusobacterium polymorphum. 1977194556
anaerobes in human biliary tracts.during a 2-year period, 1,892 patients underwent biliary tract surgery at the mayo clinic. both aerobic and anaerobic cultures of bile were performed in 371 of these patients. sixty-nine percent of the cultures were positive, and 41% (117) of these grew anaerobes, although they were present in pure culture only twice. mixed cultures most commonly contained four different organisms (three aerobes and one anaerobe). bacteroides fragilis was the single most commonly isolated anaerobe and ranked fou ...1977200634
juvenile periodontitis (periodontosis).juvenile periodontitis, characterized by early age of onset, rapid rate of periodontal tissue destruction and selective tooth involvement in the absence of commensurate aetiological factors, is recognized as a distinct clinical entity. the present paper describes the clinical findings and some immunological characteristics in a group of thirty-four patients under the age of thirty. clinical aspects studied included the influence of sex, race, family history and general health. immunological find ...1977267621
serological studies of bacteroides fragilis.using direct agglutination methods, a simple serological scheme for the classification of bacteroides fragilis is described. twenty strains of b. fragilis were selected by a process of sucessive screening from 151 strains obtained from various sources. o-antigens were prepared from the 20 strains, and used to raise antisera in rabbits. each of the 20 antisera reacted with its homologous antigen and eight antisera cross-reacted with other subspecies. these cross-reactions were successfully remove ...1977269199
[association of campylobacter sputorum s.s. sputorum with s. fusiformis: transfer of h2 between species]. 1977282238
[immune response in germ-free mice. establishment of human indigenous fusobacterium in germ-free mice and the role of fusobacterium as monocontaminant on the host (author's transl)]. 1977287545
hemagglutinating activity of fusobacterium nucleatum.gingival isolates of oral fusobacterium nucleatum strains (gram-negative anaerobic fusiform bacilli) have shown the characteristic ability to hemagglutinate a variety of erythrocytes (rbc) of human and animal origin. other members of the genus tested (f. necrophorus, f. varium, and f. mortiferum) displayed little if any ability to hemagglutinate rbc. the hemagglutination (ha) activity could be observed in the f. nucleatum strains with the whole cells and in most instances with sonicated preparat ...1977401771
evaluation of experimentally induced fusobacterium necrophorum infections in mice.two strains of mice, swiss webster and dba/2cr, were injected intraperitoneally or intravenously with varying dosages of fusobacterium necrophorum. the ability to eliminate the infection was assessed by quantitative enumeration of the organisms present in the blood, liver, and spleen, three- to 4-week-old dba/2cr mice were highly resistant to both routes of injection. the intraperitoneal injection of older mice failed to demonstrate a dose-effect relationship whereas an intravenous injection of ...1977403138
[etiology of perioral dermatitis].the studies of röckl und schubert supported the theory that fusobacteria are often found in perioral dermatitis. when we characterize perioral dermatitis as fusobacteriosis, we do not have any doubt that fluorinated corticosteroids represent a very important pathogenetic factor. corticoids enable opportune fusobacteria to become pathogenic. in our opinion without these bacteria the beginning of the perioral dermatitis seems impossible. other supporting causes, which are relevant to sex distribut ...1977403156
endotoxic activities of lipopolysaccharides of microorganisms isolated from an infected root canal in macaca cynomolgus.lipopolysaccharides (lps) prepared from a strain of bacteroides oralis, a strain of fusobacterium necrophorum, and a strain of f. nucleatum, all isolated from an infected root canal in monkey (macaca cynomolgus), were examined for endotoxic activities using primary skin reactions in rabbits and induction of leukocyte chemotaxis in rats. lps of b. oralis showed considerably lower ability to cause skin inflammation than lps of the fusobacteria. however, the leukotactic effect of the lps preparatio ...1977405737
clindamycin and gentamicin for aerobic and anaerobic sepsis.thirty-eight adult patients with serious pleuropulmonary, soft-tissue, bone, and intra-abdominal infections caused by combinations of aerobic, facultative, and anaerobic bacteria were treated with parenterally given clindamycin phosphate and gentamicin sulfate and surgery when appropriate. nine had associated bacteremia. in 29, infections failed to respond to other therapeutic regimens, which included penicillins, cephalosporins, aminoglycosides, and chloramphenicol. results with clindamycin and ...1977318824
[gram-negative, rod-like, asporogenic anaerobes and diseases caused by them]. 1977320581
serum antibodies to plaque bacteria in subjects with dental caries and gingivitis.correlations were sought between indices of gingival inflammation and dental caries experience and serum antibody titers to five species of oral bacteria. the material comprised 53 young adult males. a statistically significant, negative correlation was observed between the antibody titer to a pool of veillonella strains and dental caries experience. multiple regression analyses failed to reveal significant associations between periodontal disease and serum antibody titers. however, the data sug ...1977320647
the predominant cultivable microflora of advanced periodontitis.a study on the predominant cultivable microorganisms harbored in the base of deep periodontal pockets of eight patients aged 34-48 years was carried out using the roll tube culture technique. from a total of 475 isolates, 425 (89.5%) were obligate anaerobes, 356(74.9%) were gram-negative, and 441(92.8%) were categorized as rods. bacteroides melaninogenicus and fusobacterium nucleatum constituted the majority of the isolates in seven samples, but their mutual proportions differed considerably bet ...1977320648
inhibition of leukocyte migration by the agarose plate technique. application to antigen from candida albicans and fusobacterium nucleatum.leukocyte migration inhibition by antigen from candida albicans and fusobacterium nucleatum was studied in man by means of the direct leukocyte migration agarose technique (lmat). antigens were prepared by prolonged ultrasonication and were added to the leukocyte cultures in the original as well as in the concentrated solutions. significant inhibition of migration with a 10-fold concentrated solution of candida antigen was demonstrated in subjects showing apositive intracutaneous reaction to can ...1977320806
therapy for experimental intraabdominal sepsis: comparison of four cephalosporins with clindamycin plus gentamicin.an experimental model of intraabdominal sepsis in rats was used for evaluation of treatment with four cephalosporins: cephalothin, cefazolin, cefoxitin, and cefamandole. doses were 115 mg of each of the cephalosporins/kg and, in additional experiments, 350 mg of cephalothin or cefazolin/kg. response to treatment was evaluated by mortality rate and the incidence of intraabdominal abscess. all four cephalosporins and gentamicin produced a similar reduction in mortality rates. there was a marked va ...1977321707
continuous culture of mixed oral flora on hydroxyapatite-coated glass beads.methods for initiating and perpetuating a culture of mixed oral flora on hydroxyapatite (ht)-coated glass beads are described. preliminary characterization of the resultant flora showed that species common in human dental plaque were present. the composition of the flora could be manipulated by altering cultural conditions. scanning electron micrographs demonstrated that the microbes grew in densely packed microcolonies on the surface of the ht bead. a procedure for continuous culture of coloniz ...1977322612
the culture of clinical specimens for anaerobic bacteria: a comparison of three regimens.three procedures for the anaerobic culture of clinical specimens are compared: (i) a conventional bench routine, (ii) a system whereby primary anaerobic plates were incubated for 48 h before examination, and (iii) an anaerobic chamber. it was shown that the anaerobic chamber was of no advantage over bench regimens when primary anaerobic plates were incubated for 48 h before being examined.1977323488
parallel study of two techniques for identification of gram negative anaerobic bacilli.the authors have used two different methods for the identification of 45 strains of stric anaerobic gram negative rods: a study of biochemical characters by the api 20 anaerobic kit, and system proposed by sutter and finegold (susceptibility to discs loaded with antibiotics associated with some simple biochemical characters). a good correlation has been observed between those two techniques. however, a precise diagnostic can be given only by a whole study of biochemical characters, as proposed b ...1977325953
synthetic disaccharide-protein antigens for production of specific 04 and 09 antisera for immunofluorescence diagnosis of salmonella.the synthetic disaccharides abequose 1 leads to a 3 mannose and tyvelose 1 leads to a 3 mannose, representative of salmonella o-antigen 4 and 9 respectively, were covalently linked to bovine serum albumin (bsa) . antisera from rabbits immunized with these immunogens were used in indirect immunofluorescence assay for the identification of group b (o-antigen 4) and d (o-antigen 9) salmonella. a total of 1030 enteric bacterial strains were tested, including 207 group b and 55 group d salmonella. th ...1977327249
lymphoproliferative response during resolution and recurrence of naturally occurring gingivitis.the lymphocyte blastogenic response in humans to oral bacterial antigens was investigated during resolution and recurrence of naturally occurring gingivitis. the following conclusions were drawn: 1. actinomyces and fusobacterium and the host responses they evoke, are associated with gingival inflammation. 2. the magnitude of the blastogenic response to these antigens is directly related to the severity of gingival inflammation. 3. scaling may cause a transient rise in the blastogenic response. 4 ...1977328846
fusobacterium necrophorum: its characteristics and role as an animal pathogen. 1977329831
gliding motility and actinomycin d sensitivity of fusobacterium nucleatum and other gram-negative rods.six strains of gram-negative anaerobic fusiform rods (fusobacterium and bacteroides spp.), isolated from deep subgingival locations in humans, were examined for (i) gliding motility in slide cultures, (iii) cell densities on nutrient agar surfaces, and (iii) sensitivity to actinomycin d. known gliding (fbt) and nonmotile (nm) strains of myxococcus xanthus served as controls for the gliding and cell density experiments. in the present study, three strains of gram-negative fusiforms from the oral ...1977332633
anticomplementary activity of fusobacterium polymorphum in normal and c4-deficient sources of guinea pig complement.fusobacterium polymorphum has been isolated from the gingival crevice in humans and has been implicated in the immunopathology of periodontal diseases. the presence of alternative complement pathway factors in gingival crevice material suggests the contribution of this process in the manifestation of the local host response. a complement consumption assay was used to show that the anticomplementary activity of a cell-wall preparation from f. polymorphum in guinea pig complement progressed optima ...1977332638
oral fusobacterium, leptotrichia and bacterionema: i. hisorical survey and taxonomic considerations. 1977370082
oral fusobacterium, leptotrichia and bacterionema: ii. pathogenicity: a review of the literature. 1977370083
studies on some characteristics of hydrogen production by cell-free extracts of rumen anaerobic bacteria.hydrogen production was studied in the following rumen anaerobes: bacteroides clostridiiformis, butyrivibrio fibrisolvens, enbacterium limosum, fusobacterium necrophorum, megasphaera elsdenii, ruminococcus albus, and ruminococcus flavefaciens. clostridium pasteurianum and escherichia coli were included for comparative purposes. hydrogen production from dithionite, dithionite-reduced methyl viologen, pyruvate, and formate was determined. all species tested produced hydrogen from dithionite-reduce ...1977558042
fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon.a total of 154 strains from 22 species of bifidobacterium, peptostreptococcus, lactobacillus, ruminococcus, coprococcus, eubacterium, and fusobacterium, which are present in high concentrations in the human colon, were surveyed for their ability to ferment 21 different complex carbohydrates. plant polysaccharides, including amylose, amylopectin, pectin, polygalacturonate, xylan, laminarin, guar gum, locust bean gum, gum ghatti, gum arabic, and gum tragacanth, were fermented by some strains from ...1977563214
lethality for mice and chick embryos, pyrogenicity in rabbits and ability to gelate lysate from amoebocytes of limulus polyphemus by lipopolysaccharides from bacteroides, fusobacterium and veillonella.phenol-water extracted lipopolysaccharides (lps) from veillonella, fusobacterium nucleatum, bacteroides fragilis and bacteroides melaninogenicus were lethal for mice and 11-days-old chick embryos, pyrogenic in rabbits, and gelated limulus amoebocyte lysate. mouse lethality was considerably enhanced by actinomycin-d. in all test systems the endotoxin activity of veillonella and fusocbacterium lps was comparable to that of lps from salmonella enteritidis, which was included as a reference endotoxi ...1977564596
susceptibility to erythromycin of anaerobes of the genera bacteroides, fusobacterium, sphaerophorus, veillonella, clostridium, corynebacterium, peptococcus, peptostreptococcus.the minimal inhibitory concentrations (mic) of erythromycin were determined by broth dilution tests for 313 anaerobic strains, most of which were clinical isolates. all the gram-positive anaerobes tested (84 peptococcaceae, including 21 peptostreptococcus anaerobius and 15 peptococcus variabilis; 65 corynebacterium acnes and 29 clostridium strains, including 13 c. perfringens) were sensitive (mic values 0.012 through 3.12 microgram erythromycin/ml); so were 111 cultures of gram-negative anaerobe ...1977580038
rabbit polymorphonuclear leukocyte migration in vitro in response to lipopolysaccharides from bacteroides, fusobacterium and veillonella.puriified lipopolysaccharides (lps) from strains of bacteroides, fusobacterium and veillonella incubated with guinea pig serum, were tested for chemotatic activity against rabbit polymorphonuclear leukocytes (pmns) in modified boyden chambers. comparisons were made to a salmonella lps (s. enteriditis s-795). submicrogram amounts of lps induced positive chemotaxis, and a typical dose-response relationship up to certain dose levels was observed. the difference in chemotactic activity between the v ...1977605803
rabbit polymorphonuclear leukocyte migration in vivo in response to lipopolysaccharides from bacteroides, fusobacterium and veillonella.subcutaneously implanted chambers in rabbits were used for testing the migration of polymorphonuclear leukocytes in response to injected lps isolated from strains of bacteroides, fusobacterium and veillonella. a salmonella lps was used as reference endotoxin. no differnece in chemotactic activity between the veillonella lps and lps from salmoneila was found. fusobacterium lps whoed insignificantly lower chemotactic capacity than the salmonella lps. the bacteroides lps were all significantly less ...1977605804
chemical structure of the lipid a component of lipopolysaccharides from fusobacterium nucleatum.the lipid a component of lipopolysaccharides from fusobacterium nucleatum fev 1 consists of beta-1',6-linked d-glucosamine disaccharides, which carry two phosphate groups: one in glycosidic and one in ester linkage. the amino groups of the glucosamine disaccharides are substituted by d-3-hydroxyhexadecanoic acid. the hydroxyl groups of the disaccharide backbone are acylated by tetradecanoic, hexadecanoic, and d-3-hydroxytetradecanoic acids. part of the ester-bound d-3-hydroxytetradecanoic acid i ...1977830651
sensitivity of bacteroidaceae to fosfomycin.the sensitivity of 116 strains of bacteroides fragilis and 60 strains of sphaerophorus to fosfomycin was studied. of the 178 strains that were tested, 156 were isolated from pathological products in our department and the remainder came from the following collections: nctc, atcc and the institut fur mikrobiologie of the university of bonn. identification was made morphologically and biochemically in accordance with the scheme which we usually employ and which has previously been published. the s ...1977832544
numbers and types of anaerobic bacteria isolated from clinical specimens since 1960.between 1960 and 1974, 826 specimens, excluding stool, urine, sputum, and blood, yielded 689 (83%) positive cultures, of which 403 (58.5%) contained anaerobic bacteria. this represents 48.8% of the total specimens cultured. isolates from 153 specimens obtained and stocked from 1965 to 1974 were reidentified by current criteria. gram-negative bacilli, primarily bacteroides, were the most frequently isolated anaerobes, being found in 70% of 153 anaerobe-positive specimens and accounting for 42% of ...1977833266
[treatment with clindamycin-2-phosphate (author's transl)]. 1977839640
[susceptibility to thiamphenicol and chloramphenicol of anaerobic bacteria (author's transl)].the in vitro susceptibility to thiamphenicol and chloramphenicol of 272 anaerobes, most of which were recent clinical isolated, was determined by broth dilution tests, with chloramphenicol, 133 anaerobic gram-negative non-sporing rods (48 bacteroides fragilis, 13 b. thetaiotaomicron, 14 b. oralis, 16 sphaerophorus varius etc.) had mic values of 0.03 through 16 microng/ml. very similar results (mic, 0.06-16 microng/ml) were obtained with thiamphenicol. in concentrations of 4 microng/ml or less ch ...1977848218
experimental infections with anaerobic bacteria in mice.experimental anaerobic infections in mice are reviewed with a description of a model of pure bacteroides fragilis infection. in this experimental situation, b. fragilis produces large subcutaneous abscesses in the groins of mice that can be diagnosed without autopsy. this infection was treated effectively with clindamycin in doses that produced levels of drug in blood similar to those attainable in humans.1977850086
in vitro susceptibility of anaerobes: comparison of clindamycin and other antimicrobial agents.the in vitro activity of clindamycin against anaerobic bacteria was compared with that of other antimicrobial agents. clindamycin remained effective against most anaerobic bacteria found in infections, although resistance to the drug was found in certain groups, such as peptococcus and clostridium species.1977850095
effects of fusobacterium necrophorum leukotoxin on rabbit peritoneal macrophages in vitro.a method to demonstrate leukotoxic activity of fusobacterium necrophorum in vitro is described. continuous dialysis sac culture system, using reduced liquid medium was used to grow f necrophorum for 7-day periods. the continuous culture dialysis filtrate contained leukotoxic substance(s) which appeared to be less than 10,000 in molecular weight, heat resistant, and stable at 4 c for at least 10 days. leukotoxic activity was demonstrated in vitro by determining the percentage of macrophages takin ...1977851282
comparative growth rates of selected anaerobic species in four commonly used broth media.generations times (g(t)) of selected anaerobic species were compared in four culture media commonly used for anaerobic bacteria to evaluate these media for growing inocula for anaerobic antibiotic susceptibility determinations. twenty-two clinical isolates of bacteroides fragilis, fusobacterium spp., clostridium perfringens, and clostridium spp. were tested in schaedler broth, supplemented lombard-dowell broth, supplemented thioglycolate broth, and supplemented peptone-yeast extract-glucose brot ...1977856002
therapeutic evaluation of minocycline and tetracycline for mixed anaerobic infection in mice.minocycline has demonstrated greater in vitro activity against anaerobic bacteria than its parent compound, tetracycline. in vivo therapeutic efficacy of the two drugs was tested against a mixed anaerobic infection in a mouse model. fusobacterium necrophorum plus f. nucleatum injected intraperitoneally produced progressive intrahepatic and occasional extrahepatic abscesses, which were measured at autopsy. three treatment regimens were tested, single daily doses of antibiotic being administered b ...1977856014
[in vitro susceptibility of bacteroidaceae to cefoxitin and cephalothin (author's transl)].the activity of the new cephamycin c derivatives cefoxitin against 102 clinical isolates of bacteroidaceae (95 bacteroides strains and 7 fusobacterium-sphaerophorus cultures) was determined by tube dilution and standardized agar diffusion tests and compared to that of cephalothin. cefoxitin was more active than cephalothin against 56 bacteroides fragilis strains, 19 bacteroides thetaiota-omicron strains and two bacteroides splanchnicus strains. the difference seemed less pronounced with five bac ...1977856727
susceptibility of fusobacterium necrophorum to antimicrobials. part i: as determined by the disc method.the susceptibility of 25 isolates of fusobacterium necrophorum to 37 antimicrobials was tested using the disc method. f. necrophorum was susceptible to 15 antimicrobials, resistant to 12. to the remaining ten antimicrobials some isolates were completely resistant whereas others showed partial resistance.1977861833
the effect of temperature on growth and survival of fusobacterium necrophorum isolated from bovine liver abscesses.the ability of fusobacterium necrophorum to survive or grow in liquid nitrogen or at temperatures between -10 degrees and 59 degrees c was determined. the organism remained viable but did not grow in liquid nitrogen or between -10 degrees and 21 degrees c. it grew between 22 degrees and 43 degrees c. no isolate grew at temperatures above 43 degrees c and all three isolates survived for a minimum of 15 minutes and an average of 25 minutes at 59 degrees c. the optimum temperature for maximum growt ...1977861834
hepatic necrobacillosis. report of a case resembling metastatic tumor.a 47-year-old man had multiple pyogenic liver abscesses from which the anaerobic bacteria fusobacterium nucleatum and peptostreptococcus were isolated. the clinical course, diagnostic evaluation, and appearance of the lesions at surgery suggested metastatic tumor. histologically, the lesions consisted of necrotic liver tissue surrounded by zones of acute and chronic inflammation and fibrous connective tissue closely resembling the lesions of hepatic necrobacillosis attributable to f necrophorum. ...1977869652
penetration of the blood brain barrier by metronidazole and tinidazole. 1977873873
[outbreak of necrobacillosis of swine]. 1977878292
urease assay and urease-producing species of anaerobes in the bovine rumen and human feces.a growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that peptostreptococcus productus is often the most numerous urease-forming species in human feces. also, some fecal strains of ruminococcus albus, clostridium innocuum, and clostridium beijerinckii produced urease. single strains of fusobacterium prausnitzii, coprococcus catus, and streptococcus mitis th ...1977879770
fusobacterium in presumed actinomyces canaliculitis.based on stained smears of material obtained from the canaliculus, three cases of canaliculitis were presumed to be caused by actinomyces. cultures of these cases showed fusobacterium to be the probable infectious agent producing the disease.1977900231
fecal microbial flora in seventh day adventist populations and control subjects.a comparison of 13 vegetarian seventh day adventists with 14 nonvegetarian adventists revealed relatively few statistically significant differences in fecal flora. a separate study involved a comparison of vegetarian adventists (49 subjects), nonvegetarian adventists (45), and non-adventists on a conventional american diet (31) re: the incidence of the c. paraputrificum group in the fecal flora. the adventist groups had significantly fewer c. septicum and c. tertium isolates than the non-adventi ...1977920638
hepatic lesions and bacterial changes in mice during infection of fusobacterium necrophorum.liver abscesses were induced in male albino mice within 1 week after intraperitoneal inoculation of viable fusobacterium necrophorum la19 culture. fusobacteremia was transitory and reached a peak 2 h after inoculation then sharply declined until its disappearance 24 h post inoculation. by contrast, the number of fusobacteria in the liver increased rapidly during the first 4 h post inoculation and continued to do so less rapidly until the last sampling time (48 h post inoculation). there were sma ...1977922598
modified lombard-dowell broth as a general growth medium.a new liquid medium (modified lombard-dowell broth) was inoculated with stock culture strains of aerobic and anaerobic bacteria and compared with prereduced chopped-meat glucose inoculated with the same anaerobes. both broths were subcultured at 48 and 72 h to aerobic and anaerobic blood agar plates, and the numbers of colonies were compared after 48-h incubation of the agar plates. this was repeated with mixed cultures of both aerobes and anaerobes. for a period of 11 months all specimens recei ...1977925150
[necrobacillosis of the cattle in the irkutsk district]. 1977929951
failure to induce in rabbits effective immunity to a mixed infection of fusobacterium necrophorum and corynebacterium pyogenes with a combined bacterin.failure to induce in rabbits effective immunity to a mixed infection of fusobacterium necrophorum and corynebacterium pyogenes with a combined bacterin. onderstepoort journal of veterinary research, 44 (4), 253--2;6 (1977). rabbits were immunized with alum-precipitated, oil adjuvant and an untreated bacterin composed of f. necrophorum and c. pyogenes. immunized rabbits were challenged intradermally with a mixture of f. necrophorum and c. pyrogenes. immunized rabbits were challenged intradermally ...1977349444
dietary effects on the composition of fecal flora of rats.a long-term animal feeding experiment was conducted to compare the effect of meat and wayne laboratory chow diets on the composition of rat fecal flora. fecal bacteria were enumerated on selective media under both aerobic and anaerobic conditions. intrarectal administration of n-methyl-n' -nitro-n-nitro-soguanidine (mnng) affected the count only on the phenylethylalcohol and veillonella-neomycin agars, whereas a slightly higher number of anaerobes appeared in the feces of rats that were treated ...197716345222
bacteriological examination of summermastitis secretions. the demonstration of bacteroidaceae.corynebacterium pyogenes was isolated from 22, peptococcus indolicus from 22, a microaerophilic coccus from 21, bacteroides melaninogenicus subsp. melaninogenicus from 18, fusobacterium necrophorum from 15, and various unidentified anaerobes from 9 of a total of 22 summermastitis secretions examined.1978351551
the bacteriology of cavitating pulmonary infections and empysema. studies of transtracheal aspirates and pleural fluid.a prospective study of bacteriology in 14 cases of cavitating pulmonary infections and empyema is reported. bacteriologic results were based on transtracheal aspirates or pleural fluid. appropriate anaerobic bacteriologic methods were employed. anaerobic bacteria were recovered in 11 patients (79%); they were the only pathogens isolated in 6 patients. the predominant species were fusobacterium nucleatum, bacteroides melaninogenicus, bacteroides fragilis and peptostreptococcus. aerobic bacteria w ...1978352421
antagonism among the normal anaerobic bacteria of the mouse gastrointestinal tract determined by immunofluorescence.strictly anaerobic bacteroides sp., eubacterium sp., and fusobacterium sp. were isolated from the cecum of a conventional mouse. an immunofluorescent method utilizing rabbit antisera specific for each of these three strains was developed to determine their population levels in the gastrointestinal tracts of gnotobiotic mice. population levels of these anaerobes in groups of gnotobiotic mice colonized with either bacteroides, eubacterium, or fusobacterium were compared with those of gnotobiotes c ...1978354525
a review. lessons from an animal model of intra-abdominal sepsis.intra-abdominal sepsis that involves multiple aerobic and anaerobic bacteria derived from the colonic flora was studied in wistar rats to determine the relative roles of various microbial species. the rats challenged with pooled colonic contents showed a biphasic disease. initially, there was acute peritonitis, escherichia coli bacteremia, and high mortality. in rats that survived this acute peritonitis stage, intra-abdominal abscesses developed, and anaerobic bacteria were the preponderant orga ...1978354591
anaerobic infections in kenyatta national hospital: bacteriological isolations. 1978359316
[cerebral abscess due to fusobacterium necrophorum. importance of the conditions of the sample and of the bacteriologic analysis]. 1978368717
immunolatex localization by scanning electron microscopy of intestinal bacteria from cockroaches.an immunolatex reagent was developed from antiserum to fusobacterium varium, an obligate anaerobe isolated from the colon of the cockroach, periplaneta americana l. a new technique that enabled the preparation of a highly efficient immunolatex conjugate was used to localize the bacterium with scanning electron microscopy, in situ, in the mixed gut contents.1978623460
compact liquid nitrogen storage system yielding high recoveries of gram-negative anaerobes.a simple and compact system suitable for the preservation of fragile gram negative anaerobes and other bacteria in liquid n2 has been developed. polypropylene straws used as specimen containers can be used easily within glove bags of anaerobic chambers, and their small size greatly increases the number of cultures which can be stored. ancillary equipment and methods developed are described. the overall system was tested, using streptococcus mutans, fusobacterium nucleatum, and selenomonas sputig ...1978623475
immunoelectron microscopic localization of lipopolysaccharides in the cell wall of bacteroides oralis and fusobacterium nucleatum.lipopolysaccharides (lps) have been extracted and purified from two anaerobic gram-negative bacteria: bacteroides oralis and tfusobacterium nucleatum. chemical analysis of the preparations showed a great proportion of neutral sugars, mainly glucose, in lps of b. oralis. in rabbits, lps of b. oralis induced both immunoglobulin m and g antibodies in contrast to lps of f. nucleatum, to which only immunoglobulin m antibodies were produced. an immunohistochemical method with horseradish peroxidase-la ...1978624589
swine dysentery: studies of gnotobiotic pigs inoculated with treponema hyodysenteriae, bacteroides vulgatus, and fusobacterium necrophorum.transmission experiments were carried out in gnotobiotic pigs to determine whether lesions typical of swine dysentery could be produced by oral inoculation of treponema hyodysenteriae in combination with bacteroides vulgatus or fusobacterium necrophorum, or both. each of the organisms had been isolated from swine with early lesions of the disease. lesions were not found in 6 pigs inoculated with t hyodysenteriae alone, in 4 pigs given f necrophorum and t hyodysenteriae, or in 4 pigs given b vulg ...1978624669
immunization of mice against fusobacterium necrophorum infection by perenteral or oral administration of vaccine.immunization of mice against fusobacterium necrophorum infection was attempted by using 3 vaccination procedures: (1) intraperitoneal (ip) injection of f necrophorum cells in saline solution, (2) ip injection of cells with added aluminum hydroxide adjuvant, and (3) feeding of a powdered mouse diet containing lyophilized cells. one or 2 weekly ip injections of the bacteria cells (in saline solution) for 3, 6, or 12 weeks resulted in protection of 48.7% to 64.5% of the mice against challenge expos ...1978629432
enhancement of experimental anaerobic infections by blood, hemoglobin, and hemostatic agents.certain foreign materials have been demonstrated to enhance the infectivity of aerobic and anaerobic bacteria. whole blood and other protein compounds encountered in surgical settings or trauma were tested for their effect on infectivity of nonsporeforming anaerobic bacteria. infectious synergistic mixtures of bacteroides fragilis plus peptostreptococcus anaerobius and bacteroides melaninogenicus plus fusobacterium necrophorum were each diluted to a barely noninfectious or minimally infectious c ...1978631883
anaerobic spinal epidural abscess. case report.an acute spinal epidural abscess is reported from which a pure growth of the anaerobe fusobacterium necrophorum was isolated. the mode of infection and pathogen makes it unique. the literature concerning the bacteriology of epidural abscess and the implications of anaerobic epidural infection are discussed.1978632869
fusobacterium endocarditis treated with metronidazole. 1978638550
bacteria isolated from the duodenum, ileum, and cecum of young chicks.facultatively anaerobic and strictly anaerobic bacteria colonizing the intestinal tracts of 14-day-old chicks fed a corn-based diet were enumerated, isolated, and identified. colony counts from anaerobic roll tubes (rumen fluid medium) or aerobic plates (brain heart infusion agar) recovered from homogenates of the duodenum, upper and lower ileum, and cecum varied appreciably among samples from individual birds. anaerobic and aerobic counts from the duodenum and ileum were similar. anaerobic coun ...1978646359
intraperitoneal immunization against necrobacillosis in experimental animals.experiments employing recently developed mouse models indicated that intraperitoneal immunization with the cytoplasm (intracellular fraction) of fusobacterium necrophorum protected the animals from a lethal challenge of the pathogen. the critical immunization schedule needed to achieve complete protection involved six weekly intraperitoneal doses of the intracellular antigen. livers of immunized mice were cleared of infecting fusobacterial within 24 hours whereas those of nonimmunized mice harbo ...1978647451
[lung abscess due to fusobacterium nucleatum (author's transl)].a patient with epilepsy, 45 years of age, developed a lung abscess due to fusobacterium nucleatum which was successfully treated with intravenous lincomycin. quantitative anaerobic cultures of washed sputum proved to be useful in detecting the causative organism.1978650887
heterotrophic bacteria present in hindguts of wood-eating termites [reticulitermes flavipes (kollar)].strict anaerobic culture techniques were used to quantitate heterotrophic bacteria present in hindguts of reticulitermes flavipes. the grand mean number of viable cells per hindgut was 0.4 x 10(5) (first-instar larvae), 1.3 x 10(5) (third-instar larvae), 3.5 x 10(5) (workers), and 1.5 x 10(5) (soldiers). of a total of 344 isolates, 66.3% were streptococci that were always obtained regardless of the origin of termites, their developmental stage or caste, or their length of captivity. most of the ...1978655709
gas-lipuid chromatographic analysis of metabolic products in the identification of bacteroidaceae of clinical interest.the acid end-products of 185 isolates from the family bacteroidaceae were separated and analysed by gas-liquid chromatography on broth cultures. different media were evaluated and definitive studies were performed in a fully supplemented complex medium. the limitations of this approach to the identification of a wide range of strains from various clinical sources were determined and the results were compared with those of a series of morphological, biochemical, tolerance and antibiotic-resistanc ...1978660640
chemotypes of fusobacterium nucleatum lipopolysaccharides.lipopolysaccharides (lps) were isolated from 20 strains of fusobacterium nucleatum and examined by paper chromatography, gas liquid chromatography and colorimetric methods for the presence of neutral sugars, amino sugars and 2-keto-3-dexoxy-octonate (kdo). the lps had in common glucosamine, l-glycero-d-manno-heptose, glucose and kdo. the kdo content was low. galatose, rhamnose and d-glycero-d-manno-heptose were found in some strains. based on the sugar composition of the lps, the f. nucleatum st ...1978665242
isolation of fusobacterium necrophorum from bovine ruminal lesions. 1978671928
in vitro activity of tiamulin (81.723 hfu), a new pleuromulin derivative, against clinically significant anaerobes.the susceptibility of more than 40 strains of gram-negative and gram-positive anaerobes to tiamulin (sandoz 81.723 hfu), a new pleuromulin (pleuromutilin) derivative, was determined by broth dilution and agar dilution tests. the influences of density of the inoculum upon mics was studied by a specially designed pour plate-technique. bacteroides fragilis, b. vulgatus, b. splanchnicus, b. oralis, b. asaccharolyticus, b. melaninogenicus, fusobacterium fusiforme (f. nucleatum), sphaerophorus necroph ...1978690009
[josamycin: antibacterial activity against anaerobes (author's transl)]. 1978702806
[studies on fusobacterium species in the rumen of cattle. i. isolation of genus fusobacterium from rumen juice of cattle]. 1978703028
susceptibility of anaerobic bacteria to ten antimicrobial agents.the susceptibility pattern of 265 anaerobic bacteria from clinical isolates to 10 antimicrobial agents was investigated by the agar dilution technique. penicillin g, in a concentration of 16 mug/ml, was active against most organisms, important exceptions being 12% of bacteroides melaninogenicus and 24% of b. fragilis strains. the susceptibility of strains to ampicillin was similar to their susceptibility to penicillin g. carbenicillin, at </=128 mug/ml, inhibited all but a few strains. cefamando ...1978708014
fimbriae (pili) detected in fusobacterium necrophorum. 1978713221
swine dysentery: a perspective. 1978735944
[cases of nacrobacillosis in calves].necrobacillosis was established in 48 out of a total of 90 calves aged from 1 to 6 months, raised on a common premise. the disease affected only calves under the age of 3 months, running a more severe course with high mortality in the younger animals. along with the clinical manifestations associated with the organs involved there was also bradicardia in most of the diseased calves. nonreactive necroses were observed in the digestive organs, liver, spleen, lungs, and lymph nodes with adjacent re ...1978746618
effects of storage in an anaerobic transport system on bacteria in known polymicrobial mixtures and in clinical specimens.an anaerobic transport system (ats) which provides for catalytic removal of oxygen was evaluated by using in vitro-prepared polymicrobial mixtures of logphase bacteria and clinical specimens. inoculated swabs were stored at room temperature in (i) aerobic, (ii) anaerobic glove box, and (iii) ats environments, and bacteria were quantitated after 2, 24, 48, and 72 h. bacteria in a three-part mixture of bacteroides fragilis, peptostreptococcus anaerobius, and escherichia coli and in a five-part mix ...1978370142
toxicity of endotoxin from fusobacterium necrophorum strains to chick embryos. 1978348272
[anaerobic infections of domestic animals excluding enterotoxaemias (author's transl)].with the advent of improved techniques for anaerobic cultivation on recent years increased interest in anaerobic infections has occurred. against this background the present study presents a survey of those animal diseases (with the exception of the enterotoxaemias) in the pathogenesis of which anaerobic bacteria are involved.1978416418
[anaerobic infections of month and pharynx]. 1978284632
microbiota of gingivitis in man.a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ...1978276916
predominant microbiota associated with periodontal health in the aged. 1978281484
pyruvate, phosphate dikinase of bacteroides symbiosus. catalysis of partial reactions and formation of phosphoryl and pyrophosphoryl forms of the enzyme. 1978202599
requirement of monovalent cations for enolization of pyruvate by pyruvate, phosphate dikinase. 1978202600
septicemia and meningitis caused by fusobacterium aquatile.a case of septicemia and meningitis caused by fusobacterium aquatile is described. the insidious onset of the megningitis and occurrence of multiple cranial nerve affections were outstanding features. in spite of adequate antibiotic treatment the course was protracted, and the outcome was permanent damage to 3 of the affected cranial nerves.1978209534
[culture and establishment of new mexican strains of entamoeba histolytica].mixed and monoxenic cultures of rectal exudates from three patients with amebiasis of colon, and of feces from three asymptomatic carriers of entamoeba histolytica obtained after administration of a saline cathartic, and from a case of exudate of the perianal cutaneous lesion border produced by this parasite, were done. the culture media used were for: mixed culture, boeck and drbohlav modified culture and, for monoxenic culture, diamond's monophasic tp-s-1 medium without vitamin mixture and usi ...1978211955
comparison of fusobacterium sp. versus bacteroides sp. in the monoxenic cultivation of e. histolytica.by means of the performed studies it was established that 6 fusobacteria strains enhanced the growth of e. histolytica while 4 bacteroides strains did not possess this property.1978218400
the anticomplementary activity of lipopolysaccharide preparations and sonicates from a strain of fusobacterium nucleatum. 1978147329
abscesses of the frontal lobe of the brain secondary to covert dental sepsis.the bacterial species found in pus aspirated from brain abscesses in two patients were typical of those found in dental sepsis. subsequently apical-root abscesses were demonstrated in the upper jaws of both patients. this evidence strongly suggests that these cerebral abscesses were secondary to dental sepsis which could have spread from the teeth to the frontal lobes by several possible antaomical pathways.197879867
azo reduction of trypan blue to a known carcinogen by a cell-free extract of a human intestinal anaerobe.the azo reductase activity of a cell-free extract of fusobacterium sp. 2 is characterized using trypan blue as a substrate. either chemical reduction of this dye with sodium hydrosulfite or reduction by the cell-free extract produces a mutagenic product, o-tolidine. the o-tolidine is mutagenic in the ames salmonella/mammalian-microsome mutagenicity test when activated by a rat liver s9 preparation.1978106267
quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms.the ability of three different commercial blood culture media--brain heart infusion broth (pfizer), thiol broth (difco), and prs-peptone broth (becton, dickinson & co.)--to support the growth of five different anaerobes is described. inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. in addition, each medium was evaluated for its ability to neutralize the antibact ...197824059
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