Publications

lysozyme digestion and chemical characterization of the peptidoglycan of fusobacterium nucleatum fev 1.	the amino acids in the peptidoglycan of fusobacterium nucleatum fev 1 are d-glutamic acid, meso-lanthionine, and d- (42%) and l-alanine (58%). about 70% of the lanthionine residues were not susceptible to dinitrophenylation, evidently because they are involved in cross-linkages. consequently, lysozyme digestion of the peptidoglycan yielded 20 to 25% uncross-linked disaccharide tri- and tetrapeptides. a chemical analysis of isolated glycopeptides indicated that the structure of the building block ...	1981	6114929
aerobic and anaerobic bacteriology of peritonsillar abscess in children.	aspiration of peritonsillar abscess (quinsy) was aseptically performed in 16 children. patients' median age was 10 years (range 6 to 17 years), and 12 were males. unilateral abscess was present in all but one child. all aspirates were cultured for aerobes and anaerobes and yielded bacterial growth in all patients. anaerobes were isolated in all patients; in 3 patients (19%), they were the only organism isolated, and in 13 (81%), they were mixed with aerobes. there were 91 anaerobic isolates (5.7 ...	1981	6119870
[fibrinolytic system in human saliva activated by oral bacteria].		1981	6129013
rapid method that aids in distinguishing gram-positive from gram-negative anaerobic bacteria.	several species of anaerobic bacteria display variable gram stain reactions which often make identification difficult. a simple, rapid method utilizing a 3% solution of potassium hydroxide to distinguish between gram-positive and gram-negative bacterial was tested on 213 strains of anaerobic bacteria representing 19 genera. the gram stain reaction and koh test results were compared with the antibiotic disk susceptibilities (vancomycin and colistin) the preliminary grouping of anaerobic bacteria. ...	1981	6165736
complement-dependent histamine release from rat peritoneal mast cells, induced by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum and veillonella parvula.	the capacity of phenol/water extracted lipopolysaccharides (lps) from bacteroides, fusobacterium and veillonella to induce histamine release from rat peritoneal mast cells has been evaluated by means of a bioassay using atropinized guinea pig ileum and a fluorescence assay. histamine release was found to be completely complement dependent. it was also found that lps of veillonella have the greatest capacity to induce histamine release and bacteroides have the weakest.	1981	6168752
[combined (metal-acrylate) crowns and their effects on the periodontium from the aspect of bacteriological studies. ii].		1981	6127754
microbiota associated with the gastrointestinal tract of the common house cricket, acheta domestica.	the location and morphology of the bacteria associated with the gastrointestinal tract of acheta domestica were studied, and these bacteria were partially characterized. bacteria were associated with the peritrophic membrane in the midgut and with the gut wall and cuticular structures of the hindgut. no bacteria were associated with the fat bodies. colony-forming unit determinations indicated that there were three times more cultivatable bacteria in the hindgut than in the midgut. of these bacte ...	1981	16345692
effects of endogenous and exogenous inhibitors on the incorporation of labeled precursors into dna by human mononuclear cells.	the degree of responsiveness of lymphoid cells to activation by mitogens and antigens is commonly assessed in vitro by measuring radioactive dna precursor incorporation. several lines of evidence indicate that artifacts affect the results and that these measurements may not be an accurate reflection of cell activation. cultures of blastogenically activated lymphocytes contain soluble, noncytotoxic factors that inhibit the incorporation of radioactive nucleosides into dna by dividing cells withou ...	1982	6128306
an electron microscopic study of surface polysaccharides in bacteroides.	the electron microscopic appearance of the cell surface of bacteroides strains and klebsiella pneumoniae stained with ruthenium red or colloidal iron is described. the effect of polymyxin b (pmb) was also registered. it was found that all bacteroides strains have a polysaccharide lined 'micro-capsule' external to the outer membrane which could aggregate and form blebs. the blebs so formed were distinct from other types of bleb formed in klebsiella involving the outer membrane and induced by pmb. ...	1982	6186892
clinical and pharmacokinetic evaluation of parenteral moxalactam in infants and children.	thirty-four infants and children ranging in age from 2.5 to 180 months (mean, 40 months) were treated with parenteral moxalactam (150 mg/kg per day) for suspected or proved bacterial infections outside the central nervous system. six patients infected with haemophilus influenzae b, nine infected with staphylococcus aureus, three infected with streptococcus pneumoniae, one infected with streptococcus pyogenes, one infected with enterobacter aerogenes, one infected with fusobacterium nucleotum, an ...	1982	6214210
in vitro activity of moxalactam against anaerobic bacteria.	moxalactam was tested against 80 isolates of anaerobic bacteria from clinical specimens. the results of this study and a review of other studies, in which comparable susceptibility testing methods were used, demonstrated the susceptibility to moxalactam of members of the bacteroides fragilis group, bacteroides melaninogenicus, fusobacterium species, anaerobic gram-positive cocci, and clostridium perfringens.	1982	6218560
neutrophil chemiluminescence in response to fusobacterium nucleatum.		1982	6219208
anaerobic balanoposthitis.	to assess the causative role of non-sporing anaerobes in cass of erosive balanoposthitis, anaerobic culture was performed on purulent discharges from 104 patients with penile ulceration, a foul-smelling discharge, and a mixed and motile bacterial flora. most of 29 culturally confirmed infections were due to mixed anaerobes and eight to single anaerobes. a rapid response to treatment with metronidazole also confirmed the anaerobic cause of the infection. thus, acute anaerobic balanoposthitis can ...	1982	6121604
the bacteriology of acute necrotizing ulcerative gingivitis.	plaque samples from 22 ulcerated sites in eight patients with anug were cultured using quantitative anaerobic procedures and were examined microscopically. the partial characterization of the predominant cultivable flora revealed a constant flora comprised of a limited number of bacterial types and a variable flora composed of a heterogeneous array of bacterial types. this constant flora would appear to be pathognomonic of acute necrotizing ulcerative gingivitis (anug) and included the various t ...	1982	6122728
enhancement of cell attachment of a substrate coated with oral bacterial endotoxin by plasma fibronectin.		1982	6124591
humoral immune response to oral microorganisms in periodontitis.	serum antibody titers from patients with periodontitis were compared with those from periodontally healthy subjects. with the micro-enzyme-linked immunosorbent assay, immunoglobulin g (igg), iga, and igm antibody titers to isolates of streptococcus sanguis, actinomyces viscosus, bacteroides gingivalis, bacteroides melaninogenicus subsp. intermedius, bacteroides gingivalis, bacteroides melaninogenicus subsp. intermedius, bacteroides ochraceus, and fusobacterium nucleation were determined. antibod ...	1982	6126434
histopathology of mixed anaerobic intraabdominal infection in mice.	this study characterized acute peritonitis and chronic abscess formation resulting from experimental mixed anaerobic infection with bacteroides melaninogenicus and fusobacterium necrophorum. at intervals after infection liver and spleen samples were obtained, fixed, and processed for histological examination. an acute to chronic infection progressed in mice infected with this mixture of anaerobic bacteria, whereas, no infection resulted when either organism was injected alone. acute inflammatory ...	1982	6126612
capillary agar diffusion assay for measuring metronidazole in human gingival crevice fluid.	an agar diffusion assay in capillaries, with fusobacterium nucleatum m2 as the indicator strain, has been developed to measure metronidazole concentrations in gingival crevice fluid. the assay allows the measurement of 1.9 micrograms of metronidazole per ml in sample amounts of 0.6 microliters.	1982	6285816
[antibiotic treatment of human actinomykoses (author's transl)].	in the complex aetiology of human actinomykoses, various actinomyces species, especially a. israelii, play the pathogenetically leading role in the mixed flora so typical for these diseases. a number of other microaerophilic and anaerobic bacteria are also part of such mixed flora. since these "concomitant" bacteria are themselves potentially pathogenic, it is important to have data on antibiotic sensitivity for the entire spectrum of microorganisms, to achieve effective and economic chemotherap ...	1982	6286266
[antibacterial effects of niridazole. ii. effects on aerobic and anaerobic bacteria].	niridazole which is chemically related to metronidazole is endowed with much better antibacterial activity. first, several genera of aerobic bacteria, such as salmonella and escherichia, are susceptible to niridazole, whereas metronidazole is completely ineffective. there exist, however, some particular strains which are naturally resistant to niridazole. the in vitro activity of niridazole is still increased, if these facultative anaerobic bacteria are tested under anaerobic growth conditions. ...	1982	6299027
[septicemia due to fusobacterium necrophorum. a case report].	septicemia due to the anaerobic gram-negative bacillus fusobacterium necrophorum is exceptional. it may originate in tonsillitis or intestinal or gynecological infection. we report one case in a young man with head injury. fusobacterium necrophorum is frequently associated with aerobic pathogens such as streptococcus or staphylococcus (more than fifty per cent of the cases). metastatic localizations are numerous, often pleuro-pulmonary (infarction, abscess), hepatic (cytolysis) and meningeal (pu ...	1982	6302852
influence of sublethal antibiotic concentrations on bacterial adherence to saliva-treated hydroxyapatite.	the influence of growth in the presence of sublethal concentrations of nine antibiotics on the ability of certain potentially odontopathic bacteria to attach to saliva-treated hydroxyapatite surfaces which mimic teeth was studied. cells of actinomyces viscosus ly7 and s2, bacteroides gingivalis 381, capnocytophaga ochraceus 6, and actinobacillus actinomycetemcomitans n27 attached in lower numbers to saliva-treated hydroxyapatite when grown in the presence of 50% of the minimum inhibitory concent ...	1982	6274799
pathway of lysine degradation in fusobacterium nucleatum.	lysine was fermented by fusobacterium nucleatum atcc 25586 with the formation of about 1 mol each of acetate and butyrate. by the use of [1-14c]lysine or [6-14c]lysine, acetate and butyrate were shown to be derived from both ends of lysine, with acetate being formed preferentially from carbon atoms 1 and 2 and butyrate being formed preferentially from carbon atoms 3 to 6. this indicates that the lysine carbon chain is cleaved between both carbon atoms 2 and 3 and carbon atoms 4 and 5, with the f ...	1982	6811551
[gram negative infective endocarditis. apropos of 27 cases].		1982	6816182
the elisa system for measuring antibody reactive to fusobacterium nucleatum in the sera of patients with chronic periodontitis.	humoral igg, iga and igm responses to three typed oral strains of f. nucleatum were examined in patients with chronic periodontitis and in individuals with clinically healthy gingiva. attempts were made to isolate f. nucleatum from either the pathologic gingival crevices or healthy sulci in this study. higher levels of igg and iga reactive to all three strains were observed in the chronic periodontitis patients; however, there were no differences between the igm levels in the diseased and health ...	1982	6818334
[gram negative bacilli endocarditis ].	gram negative bacilli endocarditis are unfrequent. nevertheless we encountered 28 cases of them (8.8%) among 320 endocarditis of which 10 were primitive and 7 cases (10.9%) among 65 prosthetic endocarditis. bacterial species were 12 pseudomonas aeruginosa, 2 ps, stutzeri, 1 ps. maltophilia, 2 klebsiella pneumoniae, 2 escherichia coli, 3 serratia marcescans, 1 enterobacter cloacae, 1 brucella, 1 hemophilus aphrophilus, 1 fusobacterium funduliformis, 18 cases were hospital acquired infections rela ...	1982	6750526
[fusobacterium necrophorum associated with actinomyces odontolyticus septicemia ].	the authors have observed a case of fusobacterium necrophorum associate with actinomyces odontolyticus septicemia, which appears to be the first case reported. in a 19 year old man, 15 blood cultures allowed to isolate both germs. clinical manifestations were: acute-like abdomen, encephalitis, myocarditis and pleural effusion. treatment with amoxicillin and metronidazole was successful.	1982	6750527
attachment of cell fragments of fusobacterium nucleatum to oral epithelial cells, gingival fibroblasts and white blood cells.	the characteristic haemagglutination (ha) caused by fusobacterium nucleatum strains was used for measuring their attachment to oral epithelial, gingival fibroblast and white blood cells. whole cells and sonicated preparations of oral f. nucleatum strains vpi no. 4355, 10197 and atcc no. 10953 haemagglutinated human and sheep red blood cells. sonicated preparations of the organisms were tested for ha activity before and after absorption with human cells. buccal epithelial, gingival fibroblast and ...	1982	6751304
the use of an anaerobic incubator for the isolation of anaerobes from clinical samples.	an anaerobic incubator was compared with a standard jar system for the isolation of anaerobes from clinical material. seventy specimens were selected as likely to yield anaerobes: 342 different anaerobes were isolated in the incubator and 347 in anaebrobic jars. these included bacteroides spp (43%), peptococcus spp (26%), peptostreptococcus spp (13%), veillonella spp (7%), fusobacterium spp (7%), clostridium spp (2%) and miscellaneous gram-positive nonsporing bacilli (2%). differences in isolati ...	1982	6752210
antibiotic resistance in anaerobic bacteria: molecular biology and clinical aspects.	the patterns of antibiotic susceptibility among anaerobes isolated in the united states during the past 14 years were reviewed. resistance to the tetracyclines, the penicillins, clindamycin, and other antibiotics has emerged among strains of bacteroides, clostridium, and anaerobic cocci. genetic transfer of antibiotic resistance has been documented in anaerobic environments. inter- and intrageneric transfer by conjugation and transformation has been described. plasmids have been identified in an ...	1982	6760336
current approaches to the classification, characterisation and typing of pathogenic anaerobic bacteria.	characterisation systems based on the demonstration of soluble antigens and toxins are of much practical value but have clear taxonomic limitations. this is illustrated by reference to our work with some of the pathogenic clostridia. the definition and classification of the anaerobic cocci is a difficult and continuing challenge. systems for the classification and characterisation of the gram-negative non-sporing anaerobic bacilli are evolving well, though there are still some very difficult are ...	1982	6762653
induction of immunologic memory by a lipopolysaccharide-protein complex isolated from fusobacterium necrophorum: humoral response.	the serum antibody response in balb/c mice to a lipopolysaccharide-protein (lps-p) complex was monitored by the enzyme-linked immunosorbent assay, total and 2-mercaptoethanol-resistant hemagglutination, and radial immunodiffusion. dose-response analyses demonstrated that suitable primary doses of lps-p injected iv or im induced substantial concentrautions of specific serum immunoglobulin (ig) m and igg. moreover, these values were greatly enhanced with small-dose booster injections. inoculation ...	1982	6807141
plasmid content of some oral microorganisms isolated from subgingival plaque.	eighty-five strains of bacterial species selected from the predominant cultivable dental plaque flora of patients with different periodontal pathologies were examined for their plasmid content. microorganisms studied included: actinomyces viscosus, a. odontolyticus, bacteroides asaccharolyticus (b. gingivalis), b. melaninogenicus subspecies intermedius, and subspecies melaninogenicus, capnocytophaga ochracea (b. ochraceus), and fusobacterium nucleatum. three b. melaninogenicus isolates showed pl ...	1982	6949951
induction of immunologic memory by a lipopolysaccharide-protein complex isolated from fusobacterium necrophorum: cellular response.	the ability of a lipopolysaccharide protein (lps-p) complex extracted from fusobacterium necrophorum to establish immunologic memory in balb/c mice splenocytes was demonstrated. the lps-p molecule differed from the phenol water-extracted lps because it contained approximately 12% protein. initial experiments showed that primary and secondary spleen plaque-forming cell (pfc) responses to iv or im injections of lps-p were highly dose-dependent. suitable primary doses stimulated significant (p less ...	1982	7046528
phytotoxins.		1982	7051960
on some factors involved in virulence of bacteroides fragilis.		1982	7052015
vesicouterine fistula caused by fusobacterium necrophorum.		1982	7054335
intracranial complications of sinusitis in children. a sequela of periapical abscess.	the cases of two children with periapical abscess in the upper incisors, sinusitis, and intracranial abscess are described. the ethmoid and maxillary sinuses were involved in both patients. subdural empyema occurred in both, and one of the children had also cerebritis and brain abscess. anaerobic bacteria were isolated from the infected subdural empyemas. peptostreptococcus intermedius and microaerophilic streptococci were recovered in one patient and fusobacterium sp in the other. surgical drai ...	1982	7073175
chemoprophylaxis of anaerobic pulmonary infections. an experimental study in rabbits.	chemoprophylaxis of anaerobic pulmonary infection due to aspiration was studied in a rabbit model with the view of comparing the efficacy of procaine penicillin, clindamycin, chloramphenicol, doxycycline and cefoxitin. the antibiotic treatment was commenced immediately following transtracheal inoculation of a mixture of bacteroides fragilis, streptococcus morbillorum, fusobacterium nucleatum and eubacterium lentum. treatment was stopped after 48 h and lungs were examined for evidence of infectio ...	1982	7075325
composition of peptidoglycans in bacteroidaceae: determination and distribution of lanthionine.	peptidoglycans of organisms belonging to the strictly anaerobic family bacteroidaceae were investigated for the presence of lanthionine. different procedures for the quantitation of lanthionine were compared. performic acid and peroxide oxidation procedures on 35s-labeled peptidoglycan from fusobacterium nucleatum fev1 resulted in low yields of cysteic acid (42 and 60%, respectively) and many other additional unidentified oxidation products. lanthionine was, however, recovered in high yield (89% ...	1982	7076290
hematogenous osteomyelitis caused by fusobacterium necrophorum in a calf.		1982	7085473
studies on the antitumor activity of fusobacterium nucleatum strain ko-31.		1982	7087804
isolation and characterization of a cross-reacting antigen in strains of bacteroidaceae.	a bacteroidaceae cross-reacting antigen (bca) was isolated from several strains belonging to species in the genera fusobacterium and bacteroides. we showed that each of the 28 strains examined synthesized either bca or the o-specific lipopolysaccharide (lps). the structural difference between the two antigens was demonstrated by passive hemagglutination. bca coated erythrocytes spontaneously and reacted with equal intensity to all bacterial antisera of bca+ strains, whereas lps was species speci ...	1982	7095855
predominant microflora associated with human dental periapical abscesses.	the microflora of periapical abscesses of teeth free of periodontal disease was studied. permucosal aspiration of abscess contents and anaerobic cultural, microscopic, and biochemical techniques indicated a mixed but somewhat specific and relatively limited facultative and obligate anaerobic flora with fusobacterium nucleatum and streptococcus mitis as a frequent pair.	1982	7096563
comparative in vitro leukotoxin production of three bovine strains of fusobacterium necrophorum.	the in vitro leukotoxic activity of 3 bovine isolates of fusobacterium necrophorum which varied in pathogenicity were compared. monolayers of mouse peritoneal macrophages were exposed to culture filtrates from each f necrophorum strain, and cell viability was determined, using the trypan blue dye exclusion test. two methods were used for production of the leukotoxin: (1) medium m-1 continuous dialysis sac cultures and (2) brain-heart infusion agar plate cultures. supernatant cultural fluids cont ...	1982	7103216
anaerobic bacterial populations on normal and diseased human biopsy tissue obtained at colonoscopy.	human epithelium was cultured to characterize differences in microbial populations between regions of normal colon and between polyps, inflammatory bowel disease, and cancer and their respective adjacent normal mucosa. twenty-one patients (12 polyps, 5 inflammatory bowel disease, 4 cancer) underwent colonoscopy with anaerobic culture of mucosal biopsies from normal and diseased ascending, transverse, descending, and sigmoid colon. no differences for total number of organisms and recovery of spec ...	1982	7103479
gas-liquid chromatography of positive blood cultures for rapid presumptive diagnosis of anaerobic bacteremia.	gas-liquid chromatographic analysis of volatile fatty acid patterns was performed when growth was initially detected in 87 positive blood cultures. isovaleric acid, butyric acid, or both were found in 39 of 43 (90%) blood cultures that contained anaerobes but were absent in all 44 cultures that contained facultatively anaerobic or aerobic bacteria. the detection of isovaleric acid indicated the presence of bacteroides fragilis, as this acid was found in 88% of unimicrobial and 75% of polymicrobi ...	1982	7107839
extraction and properties of hemagglutinin from cell wall fragments of fusobacterium nucleatum.	to study the hemagglutinin of fusobacterium nucleatum, methods were sought to solubilize and purify this component. when cells of f. nucleatum were ruptured by passage through a french press, the fragments lost virtually all ability to agglutinate human erythrocytes. extraction of the fragments with 2% triton x-100 for 30 min at 22 degrees c restored hemagglutinating activity (ha). hemagglutination by these fragments could be inhibited by arginine, as can hemagglutination by intact bacteria. tre ...	1982	7118829
surgical management of multiple hepatic abscesses using ultrasonography for sequential evaluation.		1982	7123304
pathogenic anaerobes.	anaerobes are prevalent on all mucosal surfaces and virtually all anaerobic infections are endogenous. two thirds of anaerobic infections involve five anaerobic organisms or groups--the bacteroides fragilis group, the bacteroides melaninogenicus-bacteroides asaccharolyticus group, fusobacterium nucleatum, the anaerobic cocci, and clostridium perfringens. conditions that lower the oxidation-reduction potential and disrupt the mucosal surface (eg, vascular problems, malignant neoplasms, and surger ...	1982	7125789
[lacrimal canalicular actinomycosis. diagnostic and therapeutic problems].		1982	7127635
[obscure symptoms complicate the diagnosis of brain abscess].		1982	7132495
immunochemical studies of partially hydrolyzed lipopolysaccharide from fusobacterium nucleatum atcc 10953.	fusobacterium nucleatum atcc 10953 lps was split by hydrolysis with 1 per cent acetic acid into acid-soluble polysaccharide and lipid a. gel filtration of the acid-soluble polysaccharide on bio-gel p-60 gave a high-molecular-weight fraction eluted with the void volume (vo), and a fraction eluted at 2.4 x vo. the high-molecular-weight fraction was serologically active, and contained glucose as the only sugar. the other fraction, which was serologically inactive, contained l-glycero-d-manno-heptos ...	1982	7136704
bacteriology of experimental gingivitis in young adult humans.	from replicate trials of experimental gingivitis in four periodontally healthy subjects, 166 bacterial species and subspecies were detected among 3,034 randomly selected isolates from 96 samples. of these bacteria, actinomyces naeslundii (serotype iii and phenotypically similar strains that were unreactive with available antisera), actinomyces odontolyticus (serotype i and phenotypically similar strains that were unreactive with available antisera), fusobacterium nucleatum, lactobacillus species ...	1982	7141708
anaerobic mastoiditis: a report of two cases with complications.	aerobic bacteria are responsible for most cases of suppurative otitis media/mastoiditis (om/m). as many as 20-30% of middle ear aspirates in acute otitis media, however, are reported to be sterile on aerobic culture. some of these cases may be due to anaerobic bacteria. two cases of om/m due to anaerobic bacteria are reported. the first patient had no antecedent ear disease and developed a large bezold abscess caused by fusobacterium varium. the second patient had attic retraction pouches bilate ...	1982	7144403
studies of fusobacterium necrophorum from bovine hepatic abscesses: biotypes, quantitation, virulence, and antibiotic susceptibility.	isolates of fusobacterium necrophorum were obtained from 124 bovine hepatic abscesses (in 119 cattle) and from the ruminal contents of 12 cattle. three biotypes (a, ab, and b) were found. type a isolates were most common in hepatic abscesses, and type b isolates were most common in ruminal contents. virulence studies in mice indicated that type a isolates were more pathogenic than types ab or b isolates. the difference in virulences between types a and b isolates was not related to the toxicity ...	1982	7149404
fecal bacterial flora of four nordic population groups with diverse incidence of large bowel cancer.	fecal samples were collected and biologically examined from 4 population groups, exhibiting a 3-fold range in colon cancer incidence, in denmark and finland. carrier rates and counts per gram feces of several aerobic as well as anaerobic genera, including nuclear dehydrogenase-producing clostridia, were calculated. the results obtained with the described method did not confirm a relationship between colon cancer incidence and carrier rates of intestinal bacteria.	1982	7155920
haematological findings in red-necked wallabies (protemnodon rufogrisea) with necrobacillosis.	results of blood counts on 17 red-necked wallabies (protemnodon rufogrisea) with confirmed necrobacillosis have been compared with those of 36 clinically normal animals. the most common abnormality in infected animals was a raised fibrinogen level, found in all of the cases tested. neutrophilia occurred in 10 individuals and neutrophil morphology was abnormal in 15. some infected animals showed monocytosis, raised erythrocyte sedimentation rates, high platelet counts and changes in their red cel ...	1982	7156509
[comparative study of different substrates as the nutrient base of a culture medium for bacteroides and fusobacterium].	the results of the comparative study of different substrates prepared from foodstuffs and inedible raw materials and intended for use as the nutrient base of a medium for cultivating the organisms of the genera bacteroides and fusobacterium have demonstrated that commonly used nutrient bases prepared from meat, fish, casein and inedible animal blood (blood substitutes) are suitable for this purpose. the dried nutrient base prepared from the mixture of outdated blood substitutes (hydrolysin and c ...	1982	7158165
isolation and characterisation of bacteria from pyothorax (empyaemia) in cats.		1982	7164339
biotransformation of 1-nitropyrene in intestinal anaerobic bacteria.	mutagenic nitroaromatic compounds have recently been found in photocopies, urban atmosphere, automobile exhaust and wastewater. 1-nitropyrene (1-np) is readily formed when pyrene, ubiquitous in the environment, is exposed to nitrogen dioxide in the urban atmosphere or in automobile exhaust, and is highly mutagenic, inducing 449 his+ revertants/plate/nmol from salmonella typhimurium strain ta98 in the absence of s9 fraction in the salmonella-microsome test. it is possible to swallow sputum or som ...	1982	7167066
response of mice to transtracheal pulmonary inoculation of anaerobic bacteria.	the transtracheal inoculation of a mixture of anaerobic bacteria, with or without hydrochloric acid, into the lungs of mice caused nonlethal pneumonia and abscesses in a minority of challenged animals. all animals cleared organisms and recovered by 30 days. secondary infection with escherichia coli was common but did not affect the response to challenge with the anaerobes.	1982	7176528
[studies on in vitro susceptibility of various anaerobic bacteria and antimicrobial resistance of anaerobes (author's transl)].		1982	6979640
microorganisms isolated from peritonsillar abscess and indicated chemotherapy.	a bacteriologic study was made of 30 patients with peritonsillar abscesses with the use of both aerobic and anaerobic culture procedures. the abscess was punctured and the pus was aspirated by a syringe with an 18-gauge needle. aerobes and anaerobes were detected in a frequency rate of approximately 1:2. peptostreptococcus (30.2%), group a streptococci (27.9%), peptococcus (16.3%), and fusobacterium (9.3%) were preponderant pathogens. anaerobic organisms were isolated in 75% of 30 cases. aside f ...	1982	6982036
an approach to the diagnosis of anaerobic pleuropulmonary infections.		1982	6749386
comparative in vitro activities of niridazole and metronidazole against anaerobic and microaerophilic bacteria.	in vitro susceptibilities of anaerobic bacteria (bacteroides spp., fusobacterium spp., clostridium spp., and peptococcus sp.) and of campylobacter jejuni and c. coli to niridazole and metronidazole were determined. the minimum inhibitory concentrations for niridazole ranged from 0.0037 to 1.0 microgram/ml and, for metronidazole, from 0.25 to 128 micrograms/ml.	1982	6927288
n-formimidoyl thienamycin (mk0787): in vitro activity against anaerobic bacteria.	the in vitro activity of n-formimidoyl thienamycin (mk0787) was tested against 239 anaerobic bacteria clinical isolates: 70 of bacteroides fragilis, 18 of b. distasonis, 16 of b. thetaiotaomicron, 10 of b. vulgatus, 24 of bacteroides spp., 22 of b. melaninogenicus (all three subspecies), 26 of fusobacterium spp., 10 of peptococcus spp., 15 of peptostreptococcus spp., 15 of clostridium perfringens, and 13 of clostridium spp. ninety-five percent of the isolates were inhibited by less than or equal ...	1982	6952818
fusobacterium necrophorum septicemia following oropharyngeal infection.	fusobacterium necrophorum septicemia developed in five patients after an oropharyngeal infection. four patients had sore throat or neck pain, and two had findings of jugular vein septic thrombophlebitis. metastatic abscesses, including embolic pneumonia, empyema, septic arthritis, and osteomyelitis, also occurred. four patients recovered and one died. proper treatment requires recognition of the oropharyngeal source of the septicemia and its differentiation from endocarditis. antibiotic therapy ...	1982	6955528
[a study on hemagglutinating activity of oral fusobacterium (author's transl)].		1982	6955952
susceptibilities of anaerobic bacteria to n-formimidoyl thienamycin (mk0787) and to other antibiotics.	the susceptibilities of 462 clinical anaerobic bacterial isolates to n-formimidoyl thienamycin and 16 other currently available and investigational antibiotics were determined by the agar dilution technique. n-formimidoyl thienamycin was significantly more active than the reference antibiotics against most organisms tested, especially bacteroides sp., including clindamycin-resistant strains. all 462 isolates were inhibited by 4 micrograms of n-formimidoyl thienamycin per ml, and no resistant str ...	1982	6956247
inhibition of neutrophil chemotaxis by soluble bacterial products.	bacterial-neutrophil interactions may be critical determinants of virulence in periodontal diseases. this study was undertaken to examine the ability of major bacterial species from the human oral cavity to inhibit (1) peripheral blood neutrophil chemotaxis, (2) chemotactic formylmethionyl peptide binding, and (3) phagocytosis. included were cultured supernatants and sonic extracts obtained from strains of capnocytophaga, bacteroides gingivalis, fusobacterium nucleatum, bacteroides asaccharolyti ...	1982	6956714
the effect of long-term low-dose tetracycline therapy on the subgingival microflora in refractory adult periodontitis.	twenty patients were selected for antibiotic treatment due to poor response to conventional therapy. ten patients were evaluated after taking 250 mg/day of tetracycline for 2 to 7 years. ten other patients who had been on tetracycline for at least 2 years were evaluated 6 months to 2 years after stopping the antibiotic. subgingival plaque was cultured anaerobically on nonselective media (etsa) and etsa with 1 microgram/ml of tetracycline hcl. pocket depth, plaque and gingivitis were scored. thos ...	1982	6958855
circulating antibodies after experimental chronic infection in the root canal of teeth in monkeys.	root canals of 49 teeth with necrotic pulp tissue in five monkeys were infected with streptococcus faecalis ss liquefaciens, actinomyces bovis, fusobacterium nucleatum, peptostreptococcus anaerobius, and bacteroides oralis in various combinations. after 6 months the root canals and the periapical tissues were subjected to radiographic, bacteriologic, and histologic examinations. signs of periapical inflammation were radiographically registered in 41 teeth, most frequently in teeth inoculated wit ...	1982	6960463
inhibition of fusobacterium hemagglutinating activity by complement.		1982	6963555
antitumor activity in the culture supernatant fluid of fusobacterium nucleatum strain ko-31.	antitumor activity of fusobacterium nucleatum strain ko-31, which had been isolated from the oral cavity, was investigated in ehrlich carcinoma-bearing mice. the strain ko-31 was cultured in tf medium for 48 hr. one-half ml of the culture supernatant fluid was injected intraperitoneally during a 7-day period to mice with ehrlich ascites carcinoma. the survival time of the treated mice prolonged remarkably. a significant reduction in size of the solid ehrlich tumor was also achieved with the supe ...	1983	6433084
lipid a in anaerobic bacteria.	the ability of lipid a preparations from strains of bacteroides, fusobacterium, and veillonella to inhibit the lipid a-anti-lipid a reaction in an enzyme-linked immunosorbent assay was tested. anti-lipid a serum was prepared with lipid a from salmonella minnesota r595, and lipid a from escherichia coli eh100 was used as control antigen. preparations from three of four different species of bacteroides were unable to inhibit the anti-lipid a activity, whereas lipid a preparations from fusobacteriu ...	1983	6822431
cooperative formation of omega-muricholic acid by intestinal microorganisms.	three anaerobic bacteria, isolated from the ceca of rats and mice, converted, through a concerted mechanism, beta-muricholic acid, the predominant bile acid in germfree rats, into omega-muricholic acid. one isolate was a eubacterium lentum strain; the second and third isolates were tentatively identified as atypical fusobacterium sp. strains. the conversion of beta-muricholic acid into omega-muricholic acid proceeded in two steps: e. lentum oxidized the 6 beta-hydroxyl group of beta-muricholic a ...	1983	6824314
alteration in sensitivity of transmural electrical response to propionate in rat colon after chronic luminal infusion of short-chain fatty acids.	the colon is always exposed to abundant short-chain fatty acids (scfa) produced by gut fermentation. in order to know an effect of chronic load of scfa on colonic functions, we studied that the acute and chronic effects of scfa on transmural potential difference (p.d.) across the proximal colon of germ-free (gf), gnotobiotic (gb) and conventionalized (cv) rats in vivo. intravenous administration of scfa (acute effect), such as propionate, butyrate, valerate or caproate, caused a transient increa ...	1983	6827889
bacteria in enteric lesions of cattle.	thirty-nine species of bacteria were isolated from or demonstrated in the abomasal and small and large intestinal mucosa of 23 adult cattle and 41 calves and identified. the bacteria isolated were related to the gross and microscopical lesions. campylobacters, clostridium perfringens type a, c sordellii, actinobacillus lignieresii, fusobacterium necrophorum, mycobacterium paratuberculosis, aeromonas hydrophila and escherichia coli were all associated with specific lesions. the relationship of ot ...	1983	6829137
production, purification, and properties of a bacteriocin from staphylococcus aureus isolated from saliva.	staphylococci from samples of human saliva were isolated on staphylococcal-selective agar plates. these strains were tested for the inhibition of the growth of staphylococcus aureus fda209p. the frequency of inhibitory strains among all of the staphylococcal isolates was 5.2%. strain iys2, which formed the biggest inhibitory zone against the growth of the indicator strain, was used as the producer of bacteriocin. iys2 was identified to be s. aureus, based on its biological properties. the bacter ...	1983	6832811
fusobacterium nucleatum pericarditis.	a pericardial effusion was diagnosed by echocardiography in a 49 year old man who suffered acute cough, orthopnea, and chest pain. because of a positive tuberculin skin test, mycobacteria were initially suspected as the cause of the pericarditis. the patient was therefore treated with antituberculosis drugs. the pericardial effusion failed to resolve, however, and pericardiectomy was performed. culture of the pericardial fluid yielded pure fusobacterium nucleatum growth. the patient responded to ...	1983	6833485
anaerobic orbital cellulitis.	a 10-year-old boy with orbital cellulitis died seven weeks after admission to the hospital despite intensive surgical intervention and medical therapy. we isolated many anaerobic bacteria from the orbit, frontal sinus, and epidural space. orbital cellulitis, which often occurs by direct extension from infected paranasal sinuses, commonly affects children and young adults, and is a potentially lethal disease, if left untreated. heightened awareness of the role of anaerobes in chronic sinusitis an ...	1983	6838092
experimental hepatic necrobacillosis infection in cattle.	viable cultures of fusobacterium necrophorum were inoculated into the hepatic portal veins of 7 cattle. the experimentally produced hepatic necrobacillosis lesions were described from 30 minutes through 36 hours. the lesions were established by 2 different host-bacterial interactions. most frequent, microabscesses were initiated by diffusely distributed bacteria and were observed from 30 minutes through 2 hours. gross abscesses from 3 through 8 hours had centrally located abscess cores and were ...	1983	6839780
septic arthritis due to fusobacterium varium in a patient with sickle-cell anemia.		1983	6842476
bacterial populations associated with different regions of the human colon wall.	the microorganisms associated with the undiseased human colon wall were examined in material obtained from four sudden-death victims. in traffic accident subjects (aged 45 and 16 years) the anaerobe-aerobe ratio was about 10(4):1 in all areas of the colon examined, whereas in acute heart failure subjects (aged 74 and 46 years) the ratio was as low as 1.2:1. the flora of each individual was distinct and complex. although the predominant anaerobes isolated were bacteroides and fusobacterium spp., ...	1983	6847178
the therapeutic use of metronidazole in anaerobic infection: six years' experience in a london hospital.	for the past 6 years at st. thomas' hospital, metronidazole has been used to treat proven anaerobic infection at a wide variety of sites and of varying clinical severity. throughout this period, intravenous, oral, and rectal preparations of the drug have been available. initial experience was predominantly in abdominal sepsis where excellent therapeutic results were obtained and treated patients included many with mixed aerobic/anaerobic infection in whom metronidazole was used alone. metronidaz ...	1983	6849207
effect of eight growth media upon fermentation profiles of ten anaerobic bacteria.	a study was made of the influence of eight growth media upon the fermentation end-product patterns obtained with ten species of anaerobes. acidic and neutral end-products of fermentation were analysed by gas chromatography and the results were examined statistically by computer. differences in end-product profile were at least as great between different media used to grow a single anaerobic species, as between different anaerobes grown in a single type of medium. when individual fermentation end ...	1983	6853401
fusobacterium necrophorum septicemia.		1983	6854884
treatment of anaerobic infections in children with metronidazole.	the tolerance and efficacy of metronidazole were studied in 15 pediatric patients who had anaerobic infection. 5 had soft tissue abscess, 4 had aspiration pneumonia, 3 had intracranial abscess and 3 had chronic sinusitis. 45 bacterial isolates were recovered (3 isolates per patient). 40 were anaerobes and included 17 bacteroides sp. (8 sp.), 16 anaerobic cocci and 6 fusobacterium nucleatum. metronidazole was given intravenously at the dose of 30 mg/kd/day or orally in the dose of 40-50 mg/kg/day ...	1983	6861605
penicillin failure in the treatment of bacteroides fragilis lung abscess. experimental study in rabbits.	carbenicillin, chloramphenicol, doxycycline, and clindamycin were compared with penicillin for the treatment of lung abscess in an animal model produced by transtracheal inoculation of a mixture of anaerobes: bacteroides fragilis, peptococcus morbillorum, eubacterium lentum and fusobacterium nucleatum. both chloramphenicol and doxycycline eliminated the bacteria but failed to close the abscess cavity. carbenicillin, although it eradicated b. fragilis, failed to close the abscess cavity in 3 of 6 ...	1983	6872619
differentiation between gram-negative anaerobic bacteria by pyrolysis gas chromatography of lipopolysaccharides.	lipopolysaccharides extracted by phenol-water from nine strains of gram-positive anaerobic bacteria (bacteroides. fusobacterium and veillonella), have been examined by means of pyrolysis gas chromatography. lipopolysaccharides were fragmented into a group of low molecular weight components and four characteristic high molecular fractions probably consisting of hydrocarbons from the lipid part of the material. the latter fractions were specific for each of the genera tested. at the species level ...	1983	6875512
metabolism of mercapturic acid-pathway metabolites of 2-chloro-n-isopropylacetanilide (propachlor) by gastrointestinal bacteria.	1. mercapturic acid pathway metabolites of propachlor, labelled with 14c, were incubated with pig caecal contents, small and large intestinal contents, and pure cultures of gastrointestinal bacteria. 2. the glutathione, cysteine, n-acetylcysteine, and the s-oxide of the n-acetylcysteine conjugates of propachlor (2-chloro-n-isopropylacetanilide) were each metabolized by mixed pig caecal micro-organisms to 2-mercapto-n-isopropylacetanilide. 3. the extent of formation of 2-mercapto-n-isopropylaceta ...	1983	6880239
bacteremia due to fusobacterium species.	twenty-six patients were identified as having bacteremia with fusobacterium species over a five-year period at boston city hospital. they represented 0.9 percent of bacteremic patients and were equally divided as to sex. bacteremia with fusobacterium occurred primarily in young adults and in patients over 60 years of age and was not observed in children. in 16 patients (62 percent), fusobacterium was the only blood culture isolate. the most common primary foci of infection were the female genita ...	1983	6881174
[studies of mixed anaerobic infections involving bacteroides gingivalis].	the infectiousness of several combinations of bacteria containing bacteroides gingivalis and other bacteria associated with periodontal diseases was evaluated by subcutaneous injection to guinea pigs. among the seven mixtures studied, only one permitted the development of an infection easily transmissible to a second guinea pig; this bacterial mixture was composed of b. gingivalis, fusobacterium nucleatum, eubacterium saburreum, and capnocytophaga ochracea (formerly bacteroides ochraceus). owing ...	1983	6883221
necrobacillosis in wildebeest calves.		1983	6887442
identification and characterization of the major cell envelope proteins of oral strains of actinobacillus actinomycetemcomitans.	the major cell envelope protein compositions of seven actinobacillus actinomycetemcomitans strains of human origin were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the major envelope polypeptides were homogeneous, in relation to molecular weight, in all of the strains that were examined. the characterization of the five major proteins, designated env1 through env5, in the leukotoxic strain y4 revealed that proteins env2 to -5 may reside in the outer membrane as suggest ...	1983	6401694
comparison of serological reactions of typed fusobacterium nucleatum strains with those of isolates from humans, canines, and a macaca mulatta monkey.	in the present study, we compared typed f. nucleatum strains with isolates from various human periodontal diseases, canines, and a macaca mulatta monkey. all isolates displayed biochemical reactions similar to those of the typed strains in the api 20a system (analytab products, plainville, n.y.). the human and monkey isolates displayed both type i and type ii colonial morphologies on crystal violet erythromycin agar, whereas the canine isolates displayed only type i. antigen preparations of the ...	1983	6406540
[bacteriological and clinical studies in twenty patients with thoracic empyema--significance of anaerobic bacteria].		1983	6408199
isolation, partial purification and preliminary characterization of a bacteriocin from streptococcus mutans rm-10.	an extracellular bactericidal substance was isolated from the supernatant of streptococcus mutans rm-10 culture fluid and partially purified with 60% ammonium sulfate precipitation, differential centrifugation, and gel filtration on sephadex g-200. there was a good correlation of the sensitivity profiles of indicator strains whether assayed on solid medium or with purified material from cell-free culture fluid, indicating that the same inhibitory substance is produced on solid medium and in brot ...	1983	6412623
the hydrogenation of gamma-linolenic acid by pure cultures of two rumen bacteria.	two species of rumen bacteria that have been previously shown to partially hydrogenate alpha-linolenic acid have been examined for their ability to hydrogenate gamma-linolenic acid. free gamma-linolenic acid is hydrogenated in vitro to stearic acid by a rumen fusocillus sp. (n.c.i.b. 11026), but only to cis,trans-octadec-6,11-enoic acid by a butyrivibrio sp. the sequential hydrogenations are preceded by a delta 12-cis-delta 11-trans isomerization identical with that observed in the hydrogenation ...	1983	6318740