| release of endogenous noradrenaline from the vascularly perfused rat stomach in vitro: modulation by pre- and postsynaptic adrenoceptors. | we developed an experimental in vitro model to detect a very small amount of endogenous noradrenaline (na) released from the rat gastric sympathetic nerve terminals. the stomach was perfused via celiac artery with modified krebs-ringer solution containing 10 mm pargyline and 0.1% bovine serum albumin at a constant flow of 4 ml/min. the right greater splanchnic (spl) nerve (preganglionic nerve of the gastric sympathetic nerve) was stimulated electrically with square-wave pulses of 2 msec duration ... | 1992 | 1346643 |
| failure of chloramphenicol therapy in penicillin-resistant pneumococcal meningitis. | first-line therapy for meningitis is often penicillin plus chloramphenicol. penicillin-resistant streptococcus pneumoniae (prsp) infections are increasing world wide, but the efficacy of chloramphenicol for prsp meningitis is unknown. we therefore prospectively assessed children with pneumococcal meningitis treated with penicillin plus chloramphenicol over 27 months to compare outcome of penicillin-susceptible s pneumoniae (pssp) meningitis with that of prsp meningitis. 68 children with pneumoco ... | 1992 | 1346668 |
| phenotypic and functional studies on ocular t cells during herpetic infections of the eye. | herpetic stromal keratitis an inflammatory disease of the eye resulting from herpes simplex virus type 1 infection, is a common cause of blindness. the disease is generally considered to represent an immunopathologic response, but the exact mechanism remains in doubt and is subject to debate. we have investigated the nature of inflammatory cells in the eye and have isolated ocular cells to establish their phenotype and determine some of their functions. by means of immunocytochemistry and cytofl ... | 1992 | 1347309 |
| synthetic peptides analogous to the fimbrillin sequence inhibit adherence of porphyromonas gingivalis. | fimbriae are important in the adherence of many bacterial species to the surfaces they eventually colonize. porphyromonas (bacteroides) gingivalis fimbriae appear to mediate adherence to oral epithelial cells and the pellicle-coated tooth surface. the role and contribution of fimbriae in the binding of p. gingivalis to hydroxyapatite (hap) coated with saliva as a model for the pellicle-coated tooth surface were investigated. 3h-labeled p. gingivalis or the radioiodinated purified fimbriae were i ... | 1992 | 1347762 |
| diuretic action of the novel loop diuretic torasemide in the presence of angiotensin ii or endothelin-1 in anaesthetized dogs. | the effects of torasemide (0.1 and 1 mg kg-1, i.v.) and furosemide (3 mg kg-1) on renal haemodynamics and excretory responses in the presence of angiotensin ii and endothelin-1 was examined in anaesthetized dogs. angiotensin ii or endothelin-1 was continuously infused into the renal artery throughout the experiment and a bolus of torasemide or furosemide was injected into the bracheal vein. continuous intrarenal arterial (i.r.a.) infusion of angiotensin ii, at a dose of 5 ng kg-1 min-1, increase ... | 1992 | 1350626 |
| restriction fragment length polymorphism analysis of the fimbrillin locus, fima, of porphyromonas gingivalis. | with hybridization probes derived from the fimbrial locus of porphyromonas gingivalis strain 381, fima381, restriction fragment length polymorphisms (rflps) were examined at the fimbrillin locus in 39 human and animal strains of this species. the 39 strains were subdivided into nine rflp groups (i-ix) after genomic digests were probed with the internal coding sequence of the fima381 gene. thirty-three strains showed one or more alui fragments of moderate-to-high homology (greater than or equal t ... | 1992 | 1351483 |
| immunization with porphyromonas (bacteroides) gingivalis fimbriae protects against periodontal destruction. | adhesive fimbriae from porphyromonas gingivalis are cell surface structures which may be important in the virulence of this oral pathogen and thus may serve as a critical or target antigen. immunization with highly purified 43-kda fimbrial protein protected against periodontal tissue destruction when tested in the p. gingivalis-infected gnotobiotic rat model. a similarly highly purified 75-kda cell surface component did not provide protection. heat-killed whole-cell and sonicated cell surface ex ... | 1992 | 1351883 |
| detubularization-induced contractile response change of the ileum following ileocystoplasty. | we reported previously that following ileocystoplasty the structure and pharmacologic response of the implanted ileum changes towards that of the bladder. specifically, the relaxation response to alpha adrenergic (methoxamine) and purinergic (atp) stimulation reverses to a contractile response one month after the ileal segment is surgically made part of the urinary bladder. the present study was designed to investigate possible signals for this change and also to determine whether bladder respon ... | 1992 | 1351953 |
| periodontal bone loss in mice induced by different periodontopathic organisms. | periodontal bone loss in mice orally inoculated with peptostreptococcus anaerobius, pept. magnus and actinobacillus actinomycetemcomitans was compared to that in sham-inoculated mice. six-to-8-week-old balb/c mice were inoculated with 1 x 10(5), 1 x 10(7) or 1 x 10(9) colony-forming units (c.f.u.) of bacteria in 50 microliters of medium. ten mice received each concentration of bacteria and 10 sham-inoculated mice acted as controls. five mice from each of the groups were killed 6 weeks after inoc ... | 1992 | 1353347 |
| further studies on some physical and biochemical characteristics of asaccharolytic pigmented bacteroides of feline origin. | the ultrastructure of the appendages of 24 strains of asaccharolytic pigmented bacteroides spp. of cats was studied by transmission electron microscopy. all strains examined by thin section showed abundant fimbriae, outer membrane vesicles and capsules. negative staining showed fimbriae which varied from long, fine and wavy in bact. salivosus and cat group 2 to shorter, less abundant and thicker fimbriae in cat strains of bact. gingivalis as well as type strains of porphyromonas gingivalis and p ... | 1992 | 1353758 |
| immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium. | immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ... | 1992 | 1354674 |
| three types of binding by porphyromonas gingivalis and oral bacteria to fibronectin, buccal epithelial cells and erythrocytes. | this study showed that the interaction of oral bacteria with fibronectin differed with the type of organism examined. significant binding of fibronectin was found with porphyromonas gingivalis non-fimbriated (f-) strain in comparison with the fimbriated strain (f+). however, the f+ strain adhered to buccal epithelial cells in significantly larger numbers than the f- strain. fibronectin binding and epithelial cell adherence were not associated with haemagglutinating activity. these assays clearly ... | 1992 | 1355338 |
| cd45ra and cd45ro positive cd4 cells in human peripheral blood and periodontal disease tissue before and after stimulation with periodontopathic bacteria. | flow cytometric analysis was used to examine naive and primed or memory cd4 cells extracted from periodontal lesions compared with cells from peripheral blood of healthy subjects before and after stimulation with the periodontopathic bacteria, porphyromonas gingivalis and fusobacterium nucleatum. in peripheral blood, approximately 60% and 40% of cd4 cells were cd45ro+ and cd45ra+ respectively at day 0. phytohaemagglutinin (pha) induced cd45ro expression on almost 100% of cd4 cells. however, p. g ... | 1992 | 1356262 |
| occurrence of antigen-specific b cells following oral or parenteral immunization with porphyromonas gingivalis fimbriae. | the induction and distribution of antigen-specific antibody-secreting cells in various tissues were assessed in balb/c mice immunized with the purified fimbrial protein of the porphyromonas gingivalis strain 381. groups of mice were immunized by gastric intubation of liposomes containing fimbriae and gm-53 on days 0, 1, 27, and 28. additional groups of mice were immunized with p. gingivalis fimbriae and adjuvant gm-53 in freund's incomplete adjuvant by subcutaneous injection on days 0 and 28. in ... | 1992 | 1356427 |
| heterogeneity of surfaces of subgingival bacteria as detected by zeta potential measurements. | porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans (a.a.) are gram-negative bacteria which are implicated in various forms of periodontal disease. the gram-positive peptostreptococcus micros may also play an important role. for investigation of the possible adhesion and colonization mechanisms of these organisms, the charge properties of the outermost layers of bacterial cell surfaces were studied through the measurement of zeta potentials at various ph val ... | 1992 | 1357016 |
| periodontopathic potential of two strains of porphyromonas gingivalis in gnotobiotic rats. | germ-free rats were monoinfected with porphyromonas gingivalis strains 381 or a7a1-28 for 42 or 84 days. both strains induced substantial destruction of alveolar bone and soft tissue when compared to non-infected controls, but the patterns were different. strain a7a1-28 was associated with increased activity of host collagenase and gelatinase at 42 days, whereas the activity was elevated to a lesser extent at 84 days. strain 381 showed a moderate increase in host proteinase activity at 42 days, ... | 1992 | 1359858 |
| different responses in b cells induced by porphyromonas gingivalis and fusobacterium nucleatum. | a phenotypic study had shown that gingival b cells respond differently to two periodontopathic bacteria, porphyromonas gingivalis and fusobacterium nucleatum. further investigation now shows a reduction in the percentage of ki-67 + t cells in cultures of gingival and peripheral blood mononuclear cells stimulated with p. gingivalis for 3 and 6 days, respectively, but no suppression of ki-67 expression in b cells in response to either p. gingivalis or f. nucleatum. depletion studies of cultures of ... | 1992 | 1359860 |
| pregnancy after transfer of frozen-thawed human embryos. | cryopreservation of human embryos has been successfully applied in in vitro fertilization (ivf) and embryo transfer (et) programs at the national taiwan university hospital since 1988. our preliminary results with 120 frozen-thawed embryos in 31 transfer cycles showed that the survival rate of frozen embryos was 66%. following transfer, the implantation rate and clinical pregnancy rate were 6.5% and 13%, respectively. four clinical pregnancies and one preclinical pregnancy following a frozen-tha ... | 1992 | 1360300 |
| opposing actions of tolbutamide and glibenclamide on hypoxic pulmonary vasoconstriction. | 1. we show that cromakalin and diazoxide, drugs that activate atp-sensitive potassium (katp) channels, abolish hypoxic pulmonary vasoconstriction (hpv) of isolated, perfused rat lungs. 2. glibenclamide, an inhibitor of these channels, does not affect hpv, but it reverses the relaxation caused by cromakalim and diazoxide. 3. tolbutamide, which has effects similar to glibenclamide in other tissues, paradoxically abolishes hpv, an effect reversed by glibenclamide. 4. these results suggest that: (i) ... | 1992 | 1360355 |
| detection of fimbrilin gene (fima) in porphyromonas (bacteroides) gingivalis by southern blot analysis. | | 1992 | 1361002 |
| detection of cmv-dna in cells from peritoneal fluid of ipd/capd patients by polymerase chain reaction. | documented viral peritonitis in peritoneal dialysis patients is rare, although up to 20% of all cases are culture negative (non-fungal, non-bacterial). cmv-infected peritoneal cells may serve as a reservoir for reinfection and/or reactivation of cmv after renal transplantation. cmv-polymerase chain reaction (cmv-pcr) amplification analysis identified cmv-dna in cells from the peritoneal dialysate of 8 patients (3 culture negative peritonitis from a total of 5 examined) and 5 asymptomatics) out o ... | 1992 | 1361801 |
| immunization with fimbrial protein and peptide protects against porphyromonas gingivalis-induced periodontal tissue destruction. | in these studies we have attempted to show that cell surface structures are critical antigens for protection against p. gingivalis-induced periodontal destruction. fimbrillin, and in particular a synthetic 20-amino-acid fimbrillin peptide, exerts a protective effect in gnotobiotic rats, thus identifying them as potentially useful in the development of a vaccine. | 1992 | 1363504 |
| virulence factors in anaerobic bacteria. | various surface structures can be expressed in bacteroides fragilis, but little is known about capsular structures in other non-spore-forming anaerobes. fimbriae have been isolated from bacteroides fragilis and porphyromonas gingivalis. the importance of iron-repressible outer membrane proteins as virulence factors in bacteroides fragilis is under study. the low endotoxic activity of bacteroides fragilis lipopolysaccharide can be attributed to the chemical composition of this organism's lipid a. ... | 1992 | 1363545 |
| correlation between cell-adherent activity and surface structure in porphyromonas gingivalis. | the cell-adherent ability of 6 strains of porphyromonas gingivalis (381, atcc 33277, su63, kd1, w50 and w83) was compared by using radiolabeled bacterial cells and human gingival fibroblasts (gin 1), human periodontal ligament fibroblasts (hplf) and human epithelial cells (ca9-22) that had been grown on collagen beads. the cell-adherent activity of these organisms varied among strains; p. gingivalis strains 381, atcc 33277 and su63 bound to the target cells at a range of 14% to 72%, but the othe ... | 1992 | 1363734 |
| sensitization of oral bacteria to killing by low-power laser radiation. | twenty-seven compounds were screened for their ability to sensitize streptococcus sanguis to killing by light from a 7.3-mw helium/neon (hene) laser. bacteria were mixed with various concentrations of the test compounds, spread over the surfaces of agar plates, and then exposed to light from the hene laser for various time periods. the plates were then incubated and examined for zones of inhibition. those compounds found to be effective photosensitizers were then tested against porphyromonas gin ... | 1992 | 1369193 |
| generation and characterization of a monoclonal antibody specific for the major thiol-activated cysteine proteinase of porphyromonas gingivalis w83. | an igm monoclonal antibody specified for the thiol-activated proteinase of the oral pathogen porphyromonas gingivalis w83 was generated. the antibody reacted with a single protein of approximate molecular mass 43 kda in outer membrane preparations of p. gingivalis. immuno-electron microscopy using the monoclonal antibody and immunogold labelling confirmed the cell surface location of the thiol-activated proteinase. the monoclonal antibody failed to detect any proteins in western blot analysis of ... | 1992 | 1369405 |
| characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis. | alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec 1.1.1.132) is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ... | 1992 | 1370473 |
| porphyromonas gingivalis fimbriae induce expression of the neutrophil chemotactic factor kc gene of mouse peritoneal macrophages: role of protein kinase c. | to account for infiltration of the periodontal tissues by neutrophils, the present study was undertaken to examine whether porphyromonas gingivalis fimbriae, important structures involved in attachment of the bacteria to periodontal tissues, induce gene expression of the neutrophil chemoattractant kc in macrophages. the fimbriae induced expression of the kc gene of mouse peritoneal macrophages in a dose-dependent fashion. the peak of kc gene expression was observed as early as 1 h after initiati ... | 1992 | 1372296 |
| epitope mapping of hemagglutinating adhesion ha-ag2 of bacteroides (porphyromonas) gingivalis. | thirteen monoclonal antibodies (mabs) directed against hemagglutinating adhesion ha-ag2 of bacteroides (porphyromonas) gingivalis were produced by immunizing mice with the relevant immunoprecipitate from crossed immunoelectrophoresis (cie). crossed immuno-affinoelectrophoresis and hemagglutination experiments confirmed that our mabs recognized a molecule able to bind erythrocytes and involved in the hemagglutination process. in immunoelectron microscopy, these mabs labelled amorphous material as ... | 1992 | 1377182 |
| the establishment of human t cell lines reactive with specific periodontal bacteria. | human t cell lines (tcls) were obtained by stimulation of peripheral blood mononuclear cells (pbmcs) with the 2 periodontopathic bacteria, porphyromonas gingivalis fdc-381 and fusobacterium nucleatum fdc-263. after the first round of stimulation and rest, the cells responded specifically to the bacteria originally used to establish each line. throughout the culture period, the responsiveness of each of the tcls to their specific bacteria increased. phenotypic analysis of the tcls revealed hetero ... | 1992 | 1383912 |
| evidence for dietary essentiality of lithium in the rat. | the purpose of this study was to determine the dietary essentiality of lithium (li) in rats. in three experiments, two types of diets were fed during growth, reproduction, and lactation. in the first experiment, dams were maintained on a corn-based diet containing 2 ng or 500 ng (controls) li/g through five successive periods of pregnancy and lactation. the offspring of dams fed the low-li diet had significantly lower weaning weights (p = 0.011), and the percent weaned was lower (p = 0.094) than ... | 1992 | 1384620 |
| growth of porphyromonas gingivalis, treponema denticola, t. pectinovorum, t. socranskii, and t. vincentii in a chemically defined medium. | a chemically defined medium, omiz (oral microbiology and immunology, zürich)-w1 was developed. medium omiz-w1 supports the long-term proliferation of a wide range of oral anaerobes, including representative strains of four treponema species and porphyromonas gingivalis. high concentrations of ascorbic acid and ammonium ions proved to be important for the growth of these organisms. t. denticola cd-1 grew in the absence of polyamines and long-chain fatty acids, t. pectinovorum and t. socranskii re ... | 1992 | 1400984 |
| actinobacillus actinomycetemcomitans, capnocytophaga and porphyromonas gingivalis in subgingival plaque of adolescents with down's syndrome. | levels of actinobacillus actinomycetemcomitans, capnocytophaga and porphyromonas gingivalis were determined in subgingival plaque samples from 37 adolescents with down's syndrome and 37 healthy controls matched with respect to age and sex. gingival inflammation, supra- and subgingival calculus, periodontal pockets ( > 4 mm) and alveolar bone loss were registered. alveolar bone loss was more frequent in down's syndrome subjects (32%) than in the controls (3%). a. actinomycetemcomitans was detecte ... | 1992 | 1408360 |
| experimentally induced infection by oral anaerobic microorganisms in a mouse model. | a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ... | 1992 | 1408362 |
| chemical stability of insulin. 3. influence of excipients, formulation, and ph. | the influence of auxiliary substances and ph on the chemical transformations of insulin in pharmaceutical formulation, including various hydrolytic and intermolecular cross-linking reactions, was studied. bacteriostatic agents had a profound stabilizing effect--phenol > m-cresol > methylparaben--on deamidation as well as on insulin intermolecular cross-linking reactions. of the isotonicity substances, nacl generally had a stabilizing effect whereas glycerol and glucose led to increased chemical ... | 1992 | 1418641 |
| anisoosmostic liver perfusion: redox shifts and modulation of alpha-ketoisocaproate and glycine metabolism. | 1) in isolated perfused rat liver, 14co2 production from [1-14c]alpha-ketoisocaproate or [1-14c]glycine as well as ketogenesis from alpha-ketoisocaproate were stimulated upon exposure to hypoosmotic perfusion media, whereas hyperosmotic exposure inhibited. the effects of anisotonicity were preserved when ketogenesis from alpha-ketoisocaproate and 14co2 production from [1-14c]glycine were already stimulated by glucagon. on the other hand, ketogenesis from tyrosine (2 mm) or octanoate (0.1 mm) wer ... | 1992 | 1418686 |
| relative effects of intraoperative tissue expansion and undermining on wound closing tensions. | intraoperative tissue expansion is advocated as a technique to facilitate closure of soft-tissue wounds with less tension than if they were closed primarily without expansion or, presumably, simple undermining. few objective studies have been done, however, to validate the technique. we specifically questioned the relative effects of intraoperative tissue expansion and undermining on wound closing tensions in an animal model. our data showed statistically significant reduction in the force requi ... | 1992 | 1418898 |
| output power and metabolic input power of skeletal muscle contracting linearly to compress a pouch in a mock circulatory system. | output power and metabolic input power values were determined for unconditioned canine latissimus dorsi (two), gastrocnemius (seven), and triceps (three) muscles contracting linearly to cause compression of a doubly valved pouch in a hydraulic model of the circulation. the motor nerves to the muscles were stimulated tetanically with 450 msec trains of 0.1 msec pulses having a frequency of 50/sec. the muscles were contracted 10, 20, 30, and 40 times per minute and pouch output in milliliters per ... | 1992 | 1434727 |
| [complement c3 and c4 levels in serum from acute viral hepatitis]. | serial measurements of c3 and c4 complement components were performed in 50 patients with acute, uncomplicated viral hepatitis, in the beginning of the symptoms and in the peaks of serum transaminases. there were 17 patients diagnosed as having hepatitis a virus (hav) infection and 33 patients diagnosed as having hepatitis b virus (hbv) infection. there were 4 women and 46 men with a mean age of 22.1 years. in the sera of 50 healthy control subjects serum c3 and c4 complement components measured ... | 1992 | 1435361 |
| immunohistochemical localization of gangliosides in enu-induced rat glioma. | biochemical studies have indicated that the structurally simple gangliosides, including gd3 and gm3, are major glycolipid components of glioma tissues. in order to clarify the localization of the gangliosides in ethylnitrosourea-induced rat glioma, an immunohistochemical study was performed using antibodies against gm1, gm3, and gd3. the results obtained in normal fetus, newborn, and adult rat brain, and also in human glioma, were compared. in fetal and newborn rat brain, gd3 was present mainly ... | 1992 | 1449052 |
| the na+/glucose cotransporter (sglt1). | an important class of na+ transport proteins is the cotransporters. they exist in bacteria and animal cells and are responsible for the "active" accumulation of sugars, amino acids, carboxylic acids and some ions, e.g., i-, cl-, and po-4, in cells. in the small intestine and renal proximal tubule the cotransporters play an important role in the transport of salt and water across the epithelia. the most well known and best characterized na+ cotransporter is the intestinal brush border na+/glucose ... | 1992 | 1449065 |
| acute glomerulonephritis in multi-drug resistant salmonella typhi infection. | | 1992 | 1459701 |
| a satellited metacentric marker chromosome in a phenotypically normal male (transsexual). | | 1992 | 1459709 |
| the mechanisms of eikenella corrodens aggregation by salivary glycoprotein and the effect of the glycoprotein on oral bacterial aggregation. | the mechanism of aggregation of eikenella corrodens 1073 with e. corrodens aggregating factor (ecaf) which was purified from submandibular-sublingual (sm-sl) saliva was investigated. heating (100 degrees c, 10 minutes) or protease treatment of e. corrodens cells abolished the aggregating activity. the aggregation was inhibited by adding n-acetyl-d-galactosamine (galnac) and saccharides which contain a galactose residue at the non-reducing end. the aggregating activity was sensitive to edta and w ... | 1992 | 1460548 |
| microbiota associated with residual clefts and neighboring teeth in patients with cleft lip, alveolus, and palate. | twenty patients with residual clefts or pronounced soft tissue grooves, treated for uni- or bilateral cleft lip, alveolus, and palate were included in this study. ten patients were recalled for dental prophylaxis at regular intervals, 10 patients were not. one microbiologic sample was obtained from the cleft area and two samples from a tooth adjacent to the cleft (sites adjacent and distant to the cleft). between the recall and the nonrecall group there were notable differences in the presence o ... | 1992 | 1472527 |
| hiv education and health education in the united states: a national survey of local school district policies and practices. | to determine the extent to which hiv education and health education policies and practices are required by school districts in the united states, a national probability sample of public school districts was surveyed by mail in 1990. of 2,150 districts selected, 78.1% responded. hiv education was required by 66.9% of districts. of these, the percentage requiring hiv education increased by grade level from 29.7% in kindergarten to 82.3% in 7th grade, then declined to 37.3% by 12th grade. districts ... | 1992 | 1479838 |
| [pneumococcus-induced septicemia in normal and splenectomized rabbits]. | a new rabbit model to study the consequences of splenectomy in host resistance to induced pneumococcal septicaemia is presented. a simple, fast, non-invasive and complication-free technique of bacterial inoculation into the rabbit airways, using transcutaneous cannulation of the trachea, is also described. sixteen normal and 24 splenectomised animals were used. the optimal dose of pneumonococcus (serotype b, type iii) was found to be 1 x 10(7) organisms. the above dose of bacteria given transtra ... | 1992 | 1479858 |
| nuclear magnetic resonance and the brain. | the first successful demonstrations of nuclear magnetic resonance (nmr) in bulk matter were reported in 1946 (bloch, hansen and packard 1946; purcell, torrey and pound 1946). since then nmr has become a widespread technique for investigating matter of all kinds. in the 1970's nmr was applied to living systems, including man, in 2 distinct approaches. one application was in the production of images (lauterbur 1973), called magnetic resonance imaging or mri, and the other in the production of nmr ... | 1992 | 1489641 |
| analysis of the glucose transporter content of islet cell lines: implications for glucose-stimulated insulin release. | glucose transport across the plasma membrane of mammalian cells is mediated by a family of homologous proteins. each glucose transporter isoform has a specific tissue distribution which relates to that tissue's demand for glucose. the beta-cells of pancreatic islets are known to express a distinct glucose transporter isoform, termed glut 2, which has a high km for glucose. in this study, we examined the glucose transporter content of normal rat islets and three beta cell lines, beta-tc, hit and ... | 1992 | 1489658 |
| demonstration of olaquindox phototoxicity in vitro. | olaquindox is an oral anti-bacterial agent used as an additive to animal food. it is known to cause photosensitization in pig breeders, probably due to a photoallergic mechanism. also, olaquindox was found to be phototoxic in animal experiments. we have evaluated the phototoxic properties of olaquindox in vitro with a photohemolysis test using different light sources. irradiation with a source rich in ultraviolet a (uva) and, to a lesser degree, with a lamp emitting mainly visible light induced ... | 1992 | 1489718 |
| [diabetic disease and periodontal disease. diabetes and periodontopathy]. | diabetic patients have been reported to be more susceptible to gingivitis and periodontitis than healthy subjects, and these diseases are commonly considered to be oral complications of diabetes. the influence of diabetes on the onset and development of periodontal disease has been studied for many years but clear agreement is still lacking on the nature of the relationship between diabetes and these oral disorders. in fact recent observational epidemiological studies suggest that diabetes shoul ... | 1992 | 1491656 |
| periodontal pathogens in the shallow pockets of immigrants from developing countries. | the aim of this study was to examine the distribution of typical periodontitis-associated microorganisms in refugees arriving from non-industrialized countries, and to relate the presence of these organisms to the periodontal condition of the subjects. thirty males between 35-44 years of age were surveyed. dental plaque, calculus, gingivitis, loss of attachment, and probing depths were recorded for all surfaces. a total of 90 microbiological samples were taken with paper points from mesial sites ... | 1992 | 1494449 |
| the breakdown and utilization of peptides by strains of fusobacterium nucleatum. | it has been claimed that most strains of fusobacterium nucleatum require peptides rather than free amino acids for growth. in contrast, we have shown that, under continuous culture conditions, all strains tested grow in a chemically defined medium (cdm). the purposes of this study were to determine whether resting cells of f. nucleatum could attack unsubstituted peptides and whether growing cells could utilize a peptide fraction prepared from a commercial peptone. resting cells cleaved all 19 pe ... | 1992 | 1494454 |
| establishment and characterization of a sv40 t-antigen immortalized human bronchial epithelial cell line. | | 1992 | 1522038 |
| localization of a porphyromonas gingivalis 26-kilodalton heat-modifiable, hemin-regulated surface protein which translocates across the outer membrane. | we recently identified a 26-kda hemin-repressible outer membrane protein (omp26) expressed by the periodontal pathogen porphyromonas gingivalis. we report the localization of omp26, which may function as a component of a hemin transport system in p. gingivalis. under hemin-deprived conditions, p. gingivalis expressed omp26, which was then lost from the surface after a shift back into hemin-rich conditions. experiments with 125i labeling of surface proteins to examine the kinetics of mobilization ... | 1992 | 1522061 |
| thermo-stability and antitumor activity on colon cancer cell lines of monoclonal anti-cea antibody-saporin immunotoxin. | eight saporin peaks were obtained from the purification of seed extracts of saponaria officinalis l. saporin peak no. 6 (sap-6) showed the highest activity in the inhibition of protein synthesis (98%) in an in vitro translation study. an immunotoxin (it) was prepared from sap-6 conjugated to a monoclonal anti-cea antibody 26/5/1 (mab b) using n-succinimidyl pyridyl dithiopropionate (spdp) and 2-iminothiolane as a cross linker. under thermal stability study by a dsc (differential scanning calorim ... | 1992 | 1524725 |
| purification and characterization of a 50-kda cysteine proteinase (gingipain) from porphyromonas gingivalis. | porphyromonas gingivalis, a gram-negative anaerobic rod, has been closely associated with the initiation and progression of periodontal disease. this organism has been shown to produce a large number of proteolytic enzymes which can degrade a variety of tissue proteins, and these are considered to be major virulence factors. one of the proteinases produced by this organism, referred to as gingipain-1, has been purified to homogeneity from p. gingivalis culture medium by a combination of gel filt ... | 1992 | 1527017 |
| activation of complement components c3 and c5 by a cysteine proteinase (gingipain-1) from porphyromonas (bacteroides) gingivalis. | complement components c3 and c5 are susceptible to limited proteolysis by an arginine-specific cysteine proteinase isolated from porphyromonas gingivalis. this bacterium is an anaerobe commonly associated with severe periodontal disease. infection by p. gingivalis is accompanied by an acute inflammatory response, complete with extensive neutrophil involvement. this prompted us to investigate a possible direct role for complement in periodontitis evoked by p. gingivalis. exposure of c3 and c5 to ... | 1992 | 1527018 |
| subgingival distribution of periodontal pathogenic microorganisms in adult periodontitis. | the association between specific plaque microorganisms and periodontal diseases has been the subject of much recent interest due to its potential importance in the diagnosis and classification of these diseases. in order to optimize microbiological tests in periodontal therapy, it is important to know how many subgingival plaque samples must be assayed from a single patient in order to ascertain infection with a periodontal pathogen. to answer this question the present study assessed the distrib ... | 1992 | 1527685 |
| serum igg reactive with oral anaerobic microorganisms associated with infections of endodontic origin. | numerous species of bacteria have been implicated with infections of endodontic origin. the purpose of this study was to compare the levels of serum igg antibodies reactive with a panel of 10 oral anaerobic microorganisms implicated in infections of endodontic origin. serum samples were collected from 4 patient groups that included healthy patients without endodontic or periodontal disease, patients with chronic adult periodontal disease, patients with endodontic disease and patients with combin ... | 1992 | 1528630 |
| somatic cell mapping of the bovine interferon-alpha receptor. | the bovine interferon-alpha receptor (boifn-alpha r) mediates the activity of bovine ifn-alpha s and ifn-beta. in addition, human ifn-alpha s have uniformly high biological activity on bovine cells. a 32p-labeled derivative of human recombinant ifn-alpha a (huifn-alpha a-p1) binds well and can form a characteristic 130-kda complex on bovine cells, but not on hamster cells. we have, therefore, analyzed the binding and covalent crosslinking of [32p]huifn-alpha a-p1 to a panel of bovine-hamster som ... | 1992 | 1535250 |
| comparison of various detection methods for periodontopathic bacteria: can culture be considered the primary reference standard? | the development of diagnostic tests for a periodontal infection raises the issue as to what the appropriate reference standard, or "gold standard," should be for the evaluation of a new test. the present research was initiated to compare the ability of several detection methods, i.e., a serial dilution anaerobic culture and/or microscopic procedure, a dna probe procedure, and immunological reagents using both an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay to detect ... | 1992 | 1537912 |
| zahn infarct of the liver resulting from occlusive phlebitis in portal vein radicles. | a 56-yr-old man had a solitary nodular mass in the right hepatic lobe. the nodule was clinically diagnosed as hepatocellular carcinoma, and a right hepatic lobectomy was performed. the nodule had a previously undescribed histological appearance, and was finally diagnosed as a zahn infarct with the additional feature of fibrosis. we think that the mass was produced by occlusive phlebitis in a medium-size portal vein radicle and its smaller radicles. this is the case of a very unique zahn infarct ... | 1992 | 1539574 |
| discrimination as a consequence of genetic testing. | genetic discrimination refers to discrimination directed against an individual or family based solely on an apparent or perceived genetic variation from the "normal" human genotype. we describe here the results of a case history study designed to assess whether or not genetic discrimination exists. using the above definition of genetic discrimination and applying stringent criteria for case selection, we find that genetic discrimination exists and is manifested in many social institutions, espec ... | 1992 | 1539589 |
| effect of formic acid or formaldehyde treatment of alfalfa silage on nutrient utilization by dairy cows. | third-cutting alfalfa with 37% dm was ensiled untreated or treated with either 2.8 g of formic acid/100 g of dm or .31 g of formaldehyde/100 g of dm and fed to lactating dairy cows in two experiments. silage treated with formic acid had the lowest ph and concentrations of npn, nh3, and total free aa. both treatments decreased rumen in vitro protein degradability but did not affect in vitro rumen plus pepsin digestibility. in trial 1, part 1, 22 holstein cows received a standard diet for 18 d pos ... | 1992 | 1541728 |
| serial doxycycline and metronidazole in prevention of recurrent periodontitis in high-risk patients. | the efficacy of metronidazole and doxycycline in preventing recurrent periodontitis was studied in 23 patients. after treatment in the previous 7 months with either bimonthly scaling and 3 weeks of systemic doxycycline (11 subjects) or scaling and placebo (12 subjects), patients were monitored for recurrent periodontitis and were scaled every 2 months. when either a periodontal abscess or greater than 2 mm loss of gingival attachment was observed, metronidazole was administered (250 mg every 8 h ... | 1992 | 1552469 |
| demonstration of tumor necrosis factor-alpha in preovulatory follicular fluid: its association with serum 17 beta-estradiol and progesterone. | in the present study we have measured the concentrations of interleukin-1 beta (il-1 beta) and tumor necrosis factor-alpha (tnf-alpha) in preovulatory follicular fluid (ff) samples obtained from patients undergoing ovulation induction with human menopausal gonadotropin/human chorionic gonadotropin. in 13 of the 24 (54%) ff samples obtained from 20 patients, tnf-alpha was detected. in contrast, il-1 beta was observed in none of the 16 samples assessed. in the samples with detectable levels of tnf ... | 1992 | 1559630 |
| messenger rna sorting in enterocytes. co-localization with encoded proteins. | this study describes the intracellular compartmentalization of three different mrnas in the polarized rat fetal enterocyte. they encode proteins that are known to be localized within different regions of the epithelial cell namely (i) the apical, membrane-bound glycoprotein, lactase-phlorizin hydrolase (lactase), (ii) the mitochondrially localized enzyme, carbamoylphosphate synthetase (cps), and (iii) the cytoplasmically localized enzyme, phosphoenolpyruvate carboxykinase (pepck). these mrnas ar ... | 1992 | 1563519 |
| [abdominal pregnancy. six personal cases]. | this study consists of six accurately diagnosed and treated cases of abdominal pregnancy over a 16-year period (1974-1989) in the obstetrics and gynecology department of cukurova university medical faculty in adana. in this study the incidence of abdominal pregnancy we found was 1 per 4,017 births. in two cases whose pregnancies continued until term, living babies were delivered. the separation of placenta was easy in four cases. however in the other two cases, the management of placenta detachm ... | 1992 | 1570458 |
| oral microbiology: current concepts in the microbiology of dental caries and periodontal disease. | the microbial flora of the mouth is highly complex, containing a wide variety of bacterial species. the most common types of oral disease, dental caries and periodontal disease, are both related to dental plaque and seem to occur when the normal balance between the microorganisms and the host is disturbed in some way. dental caries is usually associated with increased numbers of mutans streptococci and lactobacilli at the sites of disease; estimation of salivary levels of these organisms may be ... | 1992 | 1576024 |
| polymerase chain reaction analysis of renin in rat aortic smooth muscle. | controversy exists whether vascular smooth muscle cells in vivo synthesize renin, thereby providing a critical component of the hypothesized vascular renin-angiotensin system. to examine this question, we enzymatically isolated and pooled the medial layer of thoracic aortas from sprague-dawley rats that were either untreated or enalapril treated for 3 days, isolated messenger rna (mrna), and performed northern blot analysis with rat complementary dna (cdna) probes for renin, cathepsin d, and cat ... | 1992 | 1592470 |
| polymerase chain reaction analysis of renin in rat aortic smooth muscle. | controversy exists whether vascular smooth muscle cells in vivo synthesize renin, thereby providing a critical component of the hypothesized vascular renin-angiotensin system. to examine this question, we enzymatically isolated and pooled the medial layer of thoracic aortas from sprague-dawley rats that were either untreated or enalapril treated for 3 days, isolated messenger rna (mrna), and performed northern blot analysis with rat complementary dna (cdna) probes for renin, cathepsin d, and cat ... | 1992 | 1592470 |
| transforming growth factor beta induces myoblast differentiation in the presence of mitogens. | transforming growth factor beta 1 (tgf-beta 1) added to l6e9 rat skeletal myoblasts in mitogen-rich medium induces a rapid decrease in c-myc expression and delays progression through the g1 phase of the cell cycle. this growth inhibitory response is followed by cell commitment to terminal differentiation with elevated expression of myogenin muscle determination genes, induction of muscle-specific proteins, and formation of multinucleated myotubes. these results suggest that tgf-beta 1 may act as ... | 1992 | 1594628 |
| effects of bradykinin on the hemodynamics of tumor and granulating normal tissue microvasculature. | bradykinin (bk) is an important endogenous mediator of microvascular flow modulation. since the structure of the microcirculation is very different in tumor tissues than in normal tissues, bradykinin may elicit different responses in tumors. this study was designed to test the hypothesis that local administration of bradykinin increases blood flow preferentially in normal tissue relative to adjacent tumor tissue, resulting in a "vascular steal" phenomenon. microvessel diameters (d), velocities ( ... | 1992 | 1594761 |
| tissue concentrations of organochlorine compounds in common seals from the coast of northern ireland. | an epizootic of morbillivirus infection killed several hundred common seals (phoca vitulina) along the coast of northern ireland in 1988. many dead and moribund seals were submitted to the department of agriculture for northern ireland, veterinary research laboratories for necropsy. samples of blubber, liver and kidney were collected from these animals (n = 55) for analysis for a wide range of organochlorine pesticides and also total polychlorinated biphenyls (pcbs). the organochlorines found in ... | 1992 | 1594932 |
| physicochemical and structural investigation of the surfaces of some anaerobic subgingival bacteria. | the surfaces of nine clinical isolates of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, and peptostreptococcus micros and that of laboratory strain p. gingivalis w83 were studied by using contact angle measurements, x-ray photoelectron spectroscopy, infrared spectroscopy, microelectrophoresis of whole cells, and transmission electron microscopy of whole and sectioned cells. p. intermedia strains were hydrophilic, as judged from their small water contact a ... | 1992 | 1599251 |
| catheter infection caused by methylobacterium in immunocompromised hosts: report of three cases and review of the literature. | three cases of catheter infection due to methylobacterium extorquens are reported. each patient had a history of acute leukemia and was immunocompromised; two had undergone bone marrow transplantation, and the third was receiving consolidation chemotherapy. all three patients survived after removal of the central venous catheter and antibiotic treatment. the clinical features of these cases are compared with those of the 12 previously reported cases of infection due to methylobacterium species. | 1992 | 1600002 |
| a case of adult all with +i(13q) as the sole anomaly. | a case of all with +i(13q) as the sole anomaly in a 77-year-old patient is reported. the clinical and cytogenetic findings are discussed. | 1992 | 1606564 |
| glycosaminoglycan patterns in gingival proteoglycans of rat with age. | among the potential biochemical indices that are closely associated with craniofacial development are the proteoglycans. gingival segments from the palate of 4-, 6-, 8-, 12- and 18-week-old rats were incubated for 4 h in medium containing [3h]-glucosamine and [35s]-na2so4, and subjected to proteoglycan isolation and glycosaminoglycan analysis. two distinct proteoglycan fractions differing in the degree of sulphation were obtained by ion-exchange chromatography. the incorporation of both labels i ... | 1992 | 1610301 |
| analysis of site specific periodontal bacteria sampling schemes. | a great deal of controversy has existed in the periodontal literature as to whether the site or the subject should be the unit of analysis. using the site as the unit of analysis assumes that observations of sites within the same subject are independent and ignores between subject variation. the purpose of this report is to evaluate the influence that the unit of analysis has on estimating the number of necessary site specific bacterial samples from each subject. the number of bacterial samples ... | 1992 | 1625150 |
| early effects of periodontal therapy on the detection frequency of four putative periodontal pathogens in adults. | this study aimed to examine early posttreatment changes in the periodontal microflora. paper point sampling and conventional bacterial cultivation were used to monitor the effects of surgical and non-surgical periodontal therapy on the detection frequency of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, and capnocytophaga species in deep periodontal pockets. ten patients, 5 men and 5 women (mean age 44 years), with advanced periodontal disease were select ... | 1992 | 1625154 |
| effects of pentoxifylline on the cardiovascular manifestations of group b streptococcal sepsis in the piglet. | pentoxifylline (ptxf) is a methylxanthine that modifies leukocyte function and inhibits cytokine release. to evaluate its effects on the cardiovascular manifestations of sepsis secondary to group b streptococci, 14 anesthetized, mechanically ventilated piglets were studied over a 240-min period. animals were randomly assigned to a treatment group that received a ptxf bolus (20 mg/kg) followed by a continuous infusion of 5 mg/kg/h before and during group b streptococci (1 x 10(8) colony forming u ... | 1992 | 1635822 |
| [eye infections--a red eye can indicate infection]. | | 1992 | 1636299 |
| microflora and bacterial dna restriction enzyme analysis in young adults with periodontitis. | as a result of an epidemiological survey on the periodontal status of young adults, a school was found with a relatively high number of subjects showing loss of attachment (18 out of 87). in 10 of these subjects the microflora of one pocket and of the dorsum of the tongue was investigated with special reference to black-pigmented species and actinobacillus actinomycetemcomitans. prevotella intermedia was the most prevalent species, being found in 9 patients. a. actinomycetemcomitans was isolated ... | 1991 | 1645404 |
| defective neutrophil function in an insulin-dependent diabetes mellitus patients. a case report. | the objective of this study was to evaluate the polymorphonuclear leukocyte (pmn) function in a poorly controlled adult insulin-dependent diabetic patient (iddm) with severe recurrent periodontitis, while describing the microbiological and clinical findings. chemotaxis, superoxide production, and phagocytosis and killing of porphyromonas (bacteroides) gingivalis by the iddm pmn were evaluated 1 week before treatment relative to a healthy, matched control. these analyses revealed a significant (p ... | 1991 | 1651389 |
| depolarization of polymorphonuclear leukocytes by porphyromonas (bacteroides) gingivalis 381 in the absence of respiratory burst activation. | bacteroides spp. may contribute to the chronicity of mixed infections by affecting the normal functions of polymorphonuclear leukocytes (pmn). this study evaluated the physiologic and biochemical responses of human peripheral blood pmn to a variety of strains of the oral periodontal pathogen porphyromonas (bacteroides) gingivalis. strain 381 and atcc type strain 33277 caused rapid and lasting depolarization of the electrochemical potential that exists across the pmn membrane by a mechanism that ... | 1991 | 1652560 |
| effect of transferrin on the growth of porphyromonas gingivalis. | this study describes the effect of transferrin as an iron source on the growth of porphyromonas (formally bacteroides) gingivalis. bacterial growth was monitored spectrophotometrically. all strains of p. gingivalis tested grew well in medium containing transferrin. the growth of p. gingivalis depended not only on the concentration of transferrin, but also on the iron saturation level of the protein. however, growth was not stimulated with either the ferrous or ferric iron salts tested. the addit ... | 1991 | 1655849 |
| distribution of actinobacillus actinomycetemcomitans and porphyromonas gingivalis by subject age. | the possible associations between periodontitis subject age and the distribution of actinobacillus actinomycetemcomitans and porphyromonas gingivalis were examined using an extensive data bank of subgingival plaque specimens analyzed using dna probes. the results suggest that a. actinomycetemcomitans is strongly related to subjects in the youngest age group (10 to 19 years) with decreasing prevalence and concentration levels in older age groups. in contrast, p. gingivalis showed a reverse relati ... | 1991 | 1656011 |
| experimental porphyromonas gingivalis infection in nonimmune athymic balb/c mice. | the purpose of this report was to study the role of t lymphocytes following injection of porphyromonas gingivalis in a mouse abscess model. three invasive p. gingivalis isolates (atcc 53977, w83, and ajw4) were injected into athymic balb/c mice and their heterozygous (nu/+) littermates. the athymic balb/c (nu/nu) mice were able to localize the invasive p. gingivalis isolates at the injection site. by comparison, the heterozygous balb/c (nu/+) littermates developed hemorrhagic secondary lesions w ... | 1991 | 1657788 |
| roles of porphyrins and host iron transport proteins in regulation of growth of porphyromonas gingivalis w50. | porphyromonas gingivalis (bacteroides gingivalis) requires iron in the form of hemin for growth and virulence in vitro, but the contributions of the porphyrin ring structure, porphyrin-associated iron, host hemin-sequestering molecules, and host iron-withholding proteins to its survival are unknown. therefore, the effects of various porphyrins, host iron transport proteins, and inorganic iron sources on the growth of p. gingivalis w50 were examined to delineate the various types of iron molecule ... | 1991 | 1657888 |
| subgingival microflora of advanced periodontitis in the dominican republic. | a study of the predominant subgingival microflora was carried out in 24 periodontitis patients, 18 to 60 years of age, in santo domingo, dominican republic. paper point sampling, transport in vmga iii, and conventional microbiological techniques were utilized. direct microscopic examination revealed that cocci and nonmotile organisms made up 85% of the total organisms and spirochetes as little as 3%. nonselective culturing showed gram-negative organisms to constitute 53% of total isolates. fusob ... | 1991 | 1658290 |
| site selection criteria for microbiological testing of periodontal microorganisms. | microbiological testing is becoming an adjunct to the diagnosis and monitoring of periodontal patients. however, choosing which sites and how many sites among the many available in most patients is difficult. a study of 22 periodontitis patients was undertaken to attempt to provide some guidelines to these issues. all mesiobuccal sites along with sites with 4 mm or greater probing depth were sampled with endodontic points and analyzed by dna probes for p. gingivalis, p. intermedia, and a. actino ... | 1991 | 1658292 |
| the hemagglutinating adhesin ha-ag2 of bacteroides gingivalis is distinct from fimbrilin. | we carried out a series of immunoblots with antigenic preparations from the periodontal pathogen bacteroides gingivalis using antisera of restricted specificity for the hemagglutinating adhesin ha-ag2, and for the major structural subunit of the fimbriae (fimbrilin). we have been able to show that these 2 antigens are distinct. the fimbrilin subunit had an apparent molecular weight of 42 kda in all of the bacterial preparations tested. ha-ag2 occurred as a pair of bands at 43 and 49 kda in outer ... | 1991 | 1658713 |
| variance in recovery of periodontitis-associated bacteria caused by sampling technique and laboratory processing. | the influence of sampling procedure and of laboratory processing on the recovery of bacteroides gingivalis, bacteroides intermedius, and actinobacillus antinomycetemcomitans from periodontitis sites was evaluated. thirty-three adult subjects with severe periodontitis participated in the study. in all, 462 samples from 81 sites were examined. the samples were taken using the paper point technique. the cultivations were performed by use of enriched brucella agar for determination of total colony-f ... | 1991 | 1658714 |
| immunobiological activities of synthetic peptide segments of fimbrial protein from porphyromonas gingivalis. | several oligopeptide segments of fimbrial subunit protein (fimbrilin) of porphyromonas gingivalis strain 381 were synthesized and tested for immunobiological activities. peptides f3(31-50; amino acid residue numbers 31 to 50, based on the amino acid sequence of the fimbrilin proposed by dickinson et al., infect. immun., 170, 1658, 1988), f12(212-231) and f17(312-331) were found to be immunodominant epitopes of this fimbrial protein as revealed by elisa. furthermore, peptides f5(71-90) and f17(31 ... | 1991 | 1659412 |
| asymmetry in periodontal bone loss of gnotobiotic sprague-dawley rats. | in five consecutive experiments involving 78 gnotobiotic rats, significant bone loss was seen in the maxillae of those mono-infected with strains of porphyromonas gingivalis. no significant bone loss was seen in the mandibles, and when data from both jaws were combined, the significant loss in the maxillae was occasionally concealed. it is recommended, therefore, that the levels of maxillary and mandibular bone in rats be analysed separately. a possible lateral bias of periodontal bone level was ... | 1991 | 1660259 |
| differential morphological changes in human polymorphonuclear leucocytes induced by culture products of porphyromonas (bacteroides) gingivalis w50 and its colonial variants with reduced virulence. | the colonial variants of w50, derived after growth in a chemostat and previously designated w50/bp1, w50/br1 and w50/be1, produced black-, brown- and beige-pigmented colonies, respectively, and showed reductions in mouse virulence and proteolytic activity correlating with their reduced pigmentation. incubation of glass-adherent polymorphonuclear leucocytes with culture supernatants from the virulent, highly proteolytic, black-pigmented strain produced significant changes in morphology when compa ... | 1991 | 1660710 |
| relation of baseline microbial parameters to future periodontal attachment loss. | the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ... | 1991 | 1661304 |
| relation of counts of microbial species to clinical status at the sampled site. | the purpose of the present investigation was to relate clinical characteristics at a site to the frequency of detection, absolute counts and proportions of 14 subgingival species. subgingival plaque samples were removed by curette from the mesial surface of 2299 teeth in 3 healthy and 87 subjects with periodontal attachment loss. samples were dispersed, diluted and plated on trypticase soy agar supplemented with 5% sheep blood. after 7 days of anaerobic incubation, colonies were lifted onto nylo ... | 1991 | 1661305 |
| phagocytosis and adhesiveness of peripheral blood polymorphonuclear leukocytes in patients with rapidly progressive periodontitis. | phagocytosis and adhesiveness of peripheral blood polymorphonuclear leukocytes were studied in patients diagnosed with generalized rapidly progressive (form a) periodontitis. all patients belonged to a single family and were first degree relatives. in the patients' leukocytes there was a statistically significant reduction in phagocytic index and adhesiveness, in comparison with leukocytes from controls. however, adhesiveness approached control values in the presence of autologous serum, thus im ... | 1991 | 1661332 |