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[evidence of similar anti-b4 idiotypes in a3+ phenotype rabbits immunized against the b4 allotype]. 1979108029
metabolic clearance of thyrotropin-releasing hormone in the rat in hypothyroid and hyperthyroid states: comparison with serum degradation in vitro. 1979108093
the reaction of cytochrome cd1 with oxygen and peroxides [proceedings]. 1979108145
mechanisms of activation of human natural killer cells against tumor and virus-infected cells. 1979153888
subcellular effects of some anesthetic agents on rat myocardium.the effects of ether, chloroform, and halothane on calcium accumulation and atpase activity of rat heart microsomes and mitochondria as well as on myofibrillar atpase activity were investigated. chloroform and halothane depressed microsomal and mitochondrial calcium uptake and binding in a parallel fashion. ether decreased microsomal calcium binding and mitochondrial calcium uptake to varying degrees, while mitochondrial calcium binding was slightly enhanced. whereas ether had no effect, chlorof ...1979154965
adult canine myocytes: isolation, morphology and biochemical characteristics. 1979155746
inhibition of tritiated thymidine incorporation in cultured cells by rat kidney extract.kcl extract from rat kidney, rat liver, and morris hepatomas inhibited [3h]thymidine incorporation into cultured cells. tissues came from male inbred buf rats. the most pronounced inhibition was achieved with the kidney extract. protein synthesis was not inhibited during a 24-hour exposure of the cells to the inhibitor. incorporation of [3h]deoxycytidine was inhibited, as was cell growth, when the kidney kcl extract was present for several days. [3h]thymidine incorporation was inhibited almost i ...1979155753
protamine and polyarginine bacteriolysis. similarities in its mechanism with chromatin dna picnosis.protamine and polyarginine had bacteriolytic effects indicating their primary sites of action as being wall components and showing bacterial diversity genetically determined. shake-incubation was required in producing cell-lysis. studies on bacillus subtilis revealed a high polycation multiplicity per cell in lytic event displaying multihit lysing kinetics; bacteriolysis was inhibited by trypsin, pronase, purified polyanionic wall polysaccharide, and by dissociative actions of salt hypermolariti ...1979161838
detection of memory cells by chemical modification of the lymphocyte cell surface.stringent alloantigen requirements, necessary for the differentiation of human memory cells into specific secondary cytolytic t cells (2 degrees ctl), can be bypassed by chemical modification of memory cells with the mitogenic oxidising agent, galactose oxidase. treatment of memory cells generated in a long-term primary mixed lymphocyte culture with neuraminidase and galactose oxidase (nago) results in the differentiation of memory cells into 2 degrees ctl. in contrast, treatment of unprimed cel ...1979162003
acute renal haemodynamic and renin-angiotensin system responses to graded renal artery stenosis in the dog.1. the acute renal haemodynamic and renin-angiotensin system responses to graded renal artery stenosis were studied in chronically instrumented, unanaesthetized dogs. 2. stenosis was induced over 30 sec by inflation of a cuff around the renal artery to lower distal pressure to 60, 40 or 20 mmhg, with stenosis maintained for 1 hr. this resulted in an immediate fall in renal vascular resistance, but over the next 5--30 min both resistance and renal artery pressure were restored back towards preste ...1979219182
catabolite inactivation of gluconeogenic enzymes in mutants of yeast deficient in proteinase b.strains of saccharomyces cerevisiae bearing nonsense mutations in the structural gene for proteinase b (ec 3.4.22.9) have been examined for the ability to make the transition from growth on acetate to growth on glucose and for the ability to inactivate three glucoeogenic enzymes during the transition because proteinase b has been proposed by others to be responsible for the inactivation of the three enzymes during the growth transition. the mutant strains make the growth transition normally. cat ...1979228302
isolation of foot-and-mouth disease virus messenger rna from membrane-bound polyribosomes and characterization of its 5' and 3' termini. 1979228483
regulation of rat brainstem tryptophan 5-monooxygenase. calcium-dependent reversible activation by atp and magnesium. 1979228605
cultures from rat brain hemispheres enriched in oligodendrocyte-like cells. 1979228801
studies in vivo with angiotensin i converting enzyme (kininase ii) inhibitors. 1979228990
lethal effect of complement and lysozyme on polymyxin-treated, serum-resistant gram-negative bacilli.when genetically serum-resistant escherichia coli, klebsiella pneumoniae, and citrobacter freundii, but not pseudomonas aeruginosa or proteus mirabilis, were exposed to polymyxin b, they became susceptible to the bactericidal action of normal human and rabbit sera. in constrast, beta-lactam and aminoglycoside antibiotics did not render any serum-resistant bacteria serum-sensitive. synergy between polymyxin b and the serum bactericidal system could be demonstrated by the addition of polymyxin b t ...1979229175
anion transport in red blood cells. i. chemical properties of anion recognition sites as revealed by structure-activity relationships of aromatic sulfonic acids.the present study is concerned with the chemical factors that determine the inhibitory properties of reversible aromatic sulfonic acids on sulfate exchange system of human red blood cells. two series of compounds were tested for inhibitory potencies: benzene sulfonic acid (bs) and 2,2'-disulfonic stilbene (ds) derivatives, each series with substituent groups such as cl, oh, nh2, no2, nnn, n-acetamido, and n-benzoamido. as judged by various kinetic criteria, all congeners of bs and ds appear to h ...1979229384
adenocarcinoma in a young rabbit (an atypical case). 1979257971
techniques for fetotomy on schistosomus reflexus calves. 1979257972
an explanation of therapeutic touch using the science of unitary man. 1979259269
[patient-nurse relations as a human relation]. 1979259793
[preparation for death has several dimensions - dying from developmental failure. living infinitely? living eternally?]. 1979260302
canine and feline enteritis associated with progressively motile bacteria in fresh fecal samples. 1979260540
use of a simultaneous ligating-dividing-stapling instrument for ovariohysterectomy. 1979261005
rat liver isoenzymes in acute and chronic thioacetamide poisoning. 1979262124
changes in rat lung produced by olivine dust inhalation. 1979262291
effects of herbs and drugs during pregnancy and lactation.prior to considering the effects of herbs and drugs during pregnancy and lactation, attention is directed to the relevance of herbal medicines to human patients. when a drug is taken orally, its absorption, biotransformation, and excretion will affect its blood level. it then makes its way to the capillaries surrounding the alveoli of the breast, and the drug must cross the endothelium of the capillaries into the alveolar cells of the breast and then be excreted into the lumen with the milk. ...1979262485
au cell-surface antigen of human malignant melanoma: solubilization and partial characterization.au antigen is defined by reactions of sera from patient au with cell-surface antigens of cultured autologous melanoma cells (sk-mel-28). past studies established that no available cell type other than au melanoma expressed au antigen. by use of antibody inhibition tests for antigen detection, limited papain digestion of au melanoma cells was found to result in the solubilization of au antigen along with beta2-microglobulin (beta 2m) and hla allogeneic and xenogeneic specificities. comparable pap ...1979313568
multiple ig classes on rabbit b lymphocytes.rabbit pbl were studied regarding the presence of different classes of s-ig, under experimental conditions, ensuring the endogeneous origin of these proteins. about 40% of the lymphocytes are b cells (fab positive and a1 positive in a1 homozygous rabbits). igg positive lymphocytes could be found, but only using and anti dll conjugate (allotype located on the fd gamma region of igg). anti fc gamma conjugates were negative. most of the b cells are igm positive, most of these igm cells however were ...1979313666
eikenella corrodens: a new pathogen.oral infections that result from trauma or dental manipulation are often thought to be of minor importance to the practicing clinician. the majority of oral infections respond well to treathis form of simple therapy is no longer as efficacious as it has been though to be. soment with the common antibiotics without the need for laboratory culture and sensitivity. there are instances, becoming increasingly frequent, when this form of simple therapy is no longer as efficacious as it has been though ...1979375152
protein turnover and cell-cycle initiation in yeast. 1979387426
oxygen-dependent cleavage of dna by the 1,10-phenanthroline . cuprous complex. inhibition of escherichia coli dna polymerase i. 1979387784
interference of azide with cysteine biosynthesis in salmonella typhimurium.the growth inhibition of salmonella typhimurium azia mutants by sodium azide is reversed by cystine and related compounds. nadph-sulphite reductase (hydrogen-sulphide:nadp+ oxidoreductase; ec 1.8.1.2), an enzyme of cysteine biosynthesis, is inhibited in cell extracts by sodium azide. azib mutants which are able to grow in the presence of the inhibitor without cystine were isolated. about half of them were mapped in the cysk gene and have only residual activity of its product, o-acetylserine sulp ...1979387913
production of phenylacetic acid by strains of bacteroides asaccharolyticus and bacteroides gingivalis (sp. nov.).strains of bacteroides asaccharolyticus and bacteroides melaninogenicus subspecies isolated from human and animal sources were examined for the production of phenylacetic acid. b. asaccharolyticus strains isolated from sites in humans and monkeys always produced phenylacetic acid. b. asaccharolyticus strains isolated from human nonoral sites consistently failed to produce this product. this metabolic difference correlates with the genetic dichotomy recently found to exist between oral and nonora ...19806112236
local immunity to bacteroides gingivalis in periodontal disease.the histopathologic status of gingival tissue was assessed according to the criteria of page and schroeder.(5) rhodamine-labeled bacteria were used to assess the antigenic specificity of plasma cells in the tissue. examination of tissue sections revealed that 31.6% of plasma cells in the advanced lesions bound the labeled bacterium, compared with only 3.1% of the cells in the early lesions. these results indicate that local immunity to bacteroides gingivalis in greater in the more advanced than ...19806159380
antigenic studies of oral and nonoral black-pigmented bacteroides strains.antigens of several oral and nonoral strains of bacteroides asaccharolyticus (proposed classification of oral b. asaccharolyticus, bacteroides gingivalis), bacteroides melaninogenicus subsp. intermedius, b. melaninogenicus subsp. melaninogenicus, and b. melaninogenicus subsp. levii were identified in soluble preparations obtained by sonication, autoclaving, and naoh treatment of whole bacterial cells. the sonicate preparations contained the most complete representation of soluble antigens using ...19807216426
serum antibodies to oral bacteroides asaccharolyticus (bacteroides gingivalis): relationship to age and periondontal disease.an enzyme-linked immunosorbent assay microplate method was used for measuring levels of antibody specific for the oral serotype of bacteroides asaccharolyticus (bacteroides gingivalis) in serum samples obtained from umbilical cords, infants, children, periodontally normal adults, and edentulous adults. serum from patients with various periodontal diseases, including adult periodontitis, localized juvenile periodontitis, generalized juvenile periodontitis, post-localized juvenile periodontitis, a ...19817216444
an experimentally induced phlegmonous abscess by a strain of bacteroides gingivalis in guinea pigs and mice.the virulence of b. gingivalis strain w83 was studied in an experimental animal model. cells grown overnight, washed and resuspended in broth, were injected intradermally or subcutaneously in the back of guinea pigs, rats and mice. this strain proved to be very virulent, causing a severe phlegmonous abscess in guinea pigs. also in mice, which are thought to be resistant to infections with black-pigmented bacteroides strains, the same type of infection could be induced. rats proved to be rather i ...19817247390
microbial surface interactions: reduction of the haemagglutination activity of the oral bacterium fusobacterium nucleatum by absorption with streptococcus and bacteroides.oral strains of fusobacterium nucleatum showed haemagglutination (ha) of sheep red blood cells and attachment of ha-active f. nucleatum fragments to other microorganisms allowed a means of studying microbial surface interactions. ha-active sonicated fragments (sf) prepared from f. nucleatum were mixed with whole cell suspensions of 48 bacterial strains and, after incubation, the whole cells were separated from the non-absorbed fragments by differential centrifugation. attachment of f. nucleatum ...19817041865
polyclonal b-cell activation induced by extracts of gram-negative bacteria isolated from periodontally diseased sites.the objective of this research was to determine whether gram-negative bacteria frequently isolated from periodontally diseased sites contained polyclonal b-cell activators. polyclonal b-cell activation, which results in nonspecific activation of multiple b-cell clones was analyzed by a hemolysis-in-gel assay designed to detect a broad range of antibody specificities. extracts from numerous bacterial strains, including bacteroides gingivalis, bacteroides melaninogenicus subsp. melaninogenicus, b. ...19816975240
enzymatic characterization of some oral and nonoral gram-negative bacteria with the api zym system.the api zym system (analytab products, plainview, n.y.), containing 19 chromogenic substrates, was utilized semiquantitatively to detect extracellular acid and alkaline phosphatases, aminopeptidases, proteases, esterase-lipase, phosphoamidase, and glycosidases in 128 oral and nonoral isolates of black-pigmented bacteroides, actinobacillus, haemophilus aphrophilus, capnocytophaga, fusobacterium nucleatum, wolinella recta, and veillonella parvula. in the black-pigmented bacteroides group of organi ...19817026598
identification of bacteroides gingivalis by fluorescent antibody staining.reactivity with the conjugates to the 4 serogroups a, b, c and c-1 of the former taxon bacteroides melaninogenicus and with antisera to b. gingivalis and to oral strains of b. melaninogenicus subsp. intermedius, was studied by fluorescent antibody staining (fas) on strains representative of the black-pigmented bacteroides: b. asaccharolyticus, b. melaningogenicus subsp. intermedius, b. melaninogenicus subsp. melaninogenicus, b. melaninogenicus subsp. levii, b. melaninogenicus subsp. macacae and ...19817030174
black-pigmented bacteroides spp. in the human oral cavity.five healthy children under 6 years of age, five healthy adults, and 10 adult periodontitis patients were examined for the prevalence and distribution of black-pigmented bacteroides in the oral cavity. a total of 13 samples was obtained from each individual, including four supragingival and four subgingival dental plaques, dental occlusal surface, buccal mucosa, dorsal tongue, tonsil, and whole saliva. black-pigmented bacteroides were recovered from nine adult periodontitis patients. healthy adu ...19816111541
[jean lhermitte (1877-1959)]. 1981351768
an internal deletion mutant of a myosin heavy chain in caenorhabditis elegans.unc-54 i is the structural gene for a myosin heavy chain present in a major fraction of the total myosin of caenorhabditis elegans. the allele e675, which possesses a normal amount of myosin but fails to assemble thick filaments, has been shown previously to contain a novel heavy chain of molecular weight 2 x 10(5), shorter by 10(4) than the wild-type (n2) unc-54 gene product. the structural alteration of the e675 heavy chain is an internal deletion of 10(4) molecular weight near the cooh termin ...1981271956
bacteroides gingivalis antigens and bone resorbing activity in root surface fractions of periodontally involved teeth. 19826212659
effects of estradiol and progesterone on bacteroides melaninogenicus and bacteroides gingivalis.bacteroides melaninogenicus subsp. intermedius increases in the subgingival microflora during pregnancy. these studies evaluated direct interactions between hormonal steroids and oral bacteroides species. resting cell suspensions of pure cultures of plaque organisms were incubated anaerobically with [14c]estradiol and [14c]progesterone. uptake of labeled compound per microgram of bacterial protein was determined by thin-layer chromatography and liquid scintillation counting. b. melaninogenicus s ...19826119293
phagocytosis of bacteroides melaninogenicus and bacteroides gingivalis in vitro by human neutrophils. 19826124589
humoral immune response to oral microorganisms in periodontitis.serum antibody titers from patients with periodontitis were compared with those from periodontally healthy subjects. with the micro-enzyme-linked immunosorbent assay, immunoglobulin g (igg), iga, and igm antibody titers to isolates of streptococcus sanguis, actinomyces viscosus, bacteroides gingivalis, bacteroides melaninogenicus subsp. intermedius, bacteroides gingivalis, bacteroides melaninogenicus subsp. intermedius, bacteroides ochraceus, and fusobacterium nucleation were determined. antibod ...19826126434
cytotoxic activity of bacteroides gingivalis and bacteroides asaccharolyticus.culture filtrates of all eight strains of bacteroides gingivalis and all five strains of b. asaccharolyticus were toxic for vero cells. cytotoxicity was in general greater with material from cultures of b. gingivalis than from b. asaccharolyticus but none of the culture filtrates from eight strains of b. melaninogenicus showed activity in this test. the toxic material was released during prolonged incubation and more detailed study of preparations from one strain indicated that it had a molecula ...19826128424
rapid identification of bacteroides gingivalis.rapid colorimetric tests for trypsin and alpha-glucosidase are described for use in the identification of bacteroides gingivalis from dental plaque.19827040453
characterization of bacteroides gingivalis by direct fluorescent antibody staining and cellular fatty acid profiles.bacteroides gingivalis is a newly proposed species which includes strains isolated from the mouth. thirteen strains of b. gingivalis isolated from three geographic locations in the united states and france were examined with direct fluorescent antibody staining and analysis of total cellular fatty acids and compared with 16 strains of b. asaccharolyticus of nonoral origin by the same methods. bacteroides gingivalis strains reacted with the b. gingivalis conjugate (fluorescein isothiocyanate labe ...19826178489
api zym system for identification of bacteroides spp., capnocytophaga spp., and spirochetes of oral origin.a total of 80 oral strains of bacteroides gingivalis, b. asaccharolyticus, b. melaninogenicus subsp. intermedius, b. melaninogenicus subsp. melaninogenicus, capnocytophaga, treponema denticola, and t. vincentii were characterized with the api zym system for 19 enzyme activities. comparison of anaerobic and aerobic incubation with nine reference strains of these organisms showed no important differences. the key differential tests for black-pigmented bacteroides strains and treponemes of oral ori ...19826764781
butyrate: a cytotoxin for vero cells produced by bacteroides gingivalis and bacteroides asaccharolyticus.culture filtrates of b. gingivalis and b. asaccharolyticus are cytotoxic for vero cells. it is shown that the cytotoxic effect is due to the butyrate concentrations present in the culture filtrates of these strains. this cytotoxic effect proved to be reversible. strains of the b. melaninogenicus subspecies intermedius and melaninogenicus did not produce butyrate and did not show cytotoxic activity towards vero cells. the significance of the production of toxic concentrations of butyrate for the ...19827149698
long-wave uv light fluorescence for identification of black-pigmented bacteroides spp.black-pigmented bacteroides strains were grown on blood agar, and the colonies were evaluated for fluorescence from long-wave uv light. most test strains of bacteroides melaninogenicus subsp. intermedius exhibited a brilliant red fluorescence. b. melaninogenicus subsp. melaninogenicus fluoresced mostly red-orange. bacteroides asaccharolyticus showed a yellow or red fluorescence. the intensity of the bacteroides fluorescence weakened when the black pigment of the colonies developed. in contrast, ...19827161378
inhibition of neutrophil chemotaxis by soluble bacterial products.bacterial-neutrophil interactions may be critical determinants of virulence in periodontal diseases. this study was undertaken to examine the ability of major bacterial species from the human oral cavity to inhibit (1) peripheral blood neutrophil chemotaxis, (2) chemotactic formylmethionyl peptide binding, and (3) phagocytosis. included were cultured supernatants and sonic extracts obtained from strains of capnocytophaga, bacteroides gingivalis, fusobacterium nucleatum, bacteroides asaccharolyti ...19826956714
an approach to the definition of periodontal disease syndromes by cluster analysis.clinical syndromes of 22 untreated patients with advanced destructive periodontal disease were analyzed using cluster analysis. clinical characteristics coded for each patient included age, sex, measures of gingival inflammation, plaque, suppuration, pocket depth, attachment level, extent and pattern of bone loss, rate of change in pocket depth, and correlation coefficients between certain clinical measurements. microbiologic features included darkfield enumeration of 10 morphologically distinct ...19826960022
influence of sublethal antibiotic concentrations on bacterial adherence to saliva-treated hydroxyapatite.the influence of growth in the presence of sublethal concentrations of nine antibiotics on the ability of certain potentially odontopathic bacteria to attach to saliva-treated hydroxyapatite surfaces which mimic teeth was studied. cells of actinomyces viscosus ly7 and s2, bacteroides gingivalis 381, capnocytophaga ochraceus 6, and actinobacillus actinomycetemcomitans n27 attached in lower numbers to saliva-treated hydroxyapatite when grown in the presence of 50% of the minimum inhibitory concent ...19826274799
enzymatic modification of bacterial receptors on saliva-treated hydroxyapatite surfaces.certain properties of experimental pellicles formed by the adsorption of salivary components on hydroxyapatite surfaces change over time. to determine whether enzymes likely to be present in the oral environment could induce such changes, pellicles were treated with saliva which had been incubated for 18 h at 35 degrees c to promote the elaboration of microbial enzymes. this treatment markedly reduced the numbers of streptococcus mutans mt3 and jbp and s. sanguis fc-1 and c5 cells which attached ...19826281193
kinetic studies on an intramuscular iron-poly (sorbitol-gluconic acid) complex.levels of iron and unsaturated iron binding capacity in serum and the renal excretion of iron were studied in 15 anaemic patients receiving ferastral. various dose schedules were used, 100 mg fe daily for 10 days, 200 mg fe daily for 5 days, and 500 mg fe twice at various intervals from 2 to 7 days. a continuous increase of iron in serum was seen after the doses 100 mg and 200 mg daily as well as after 500 mg given at intervals of 2 and 3 days. longer 500 mg dose intervals resulted in a fluctuat ...1982272032
[a contribution to the history of czech-russian medical relations in the first half of the 19th century. iii. connections between czech and russian physicians in the scientific domain (author's transl)]. 1982347562
[attitudes of the population towards the dentist]. 1982272092
haemolysis of human erythrocytes by the fusobacterium nucleatum associated with periodontal disease.fusobacterium nucleatum and bacteroides gingivalis are associated with oral disease. they both attach to haemagglutinate and human erythrocytes. experiments were performed to determine whether haemolysis would occur following attachment of strains of f. nucleatum and bacteroides species including b. gingivalis. the f. nucleatum strains consistently displayed both haemagglutination and haemolytic activity. the b. gingivalis strains and other bacteroides species displayed haemagglutination but no ...19836579906
[glycylprolyl dipeptidylaminopeptidase in peritoneal exudates of guinea pig stimulated by bacteroides gingivalis]. 19836582174
nonspecific induction of immunoglobulin m antibodies to periodontal disease-associated microorganisms after polyclonal human b-lymphocyte activation by fusobacterium nucleatum.the production of antibodies to oral bacteria was determined in lymphocyte cultures stimulated with sonicated fusobacterium nucleatum, a potent inducer of polyclonal b-cell activation. after 9 days the cultures were examined by a microenzyme-linked immunosorbent assay for immunoglobulin m (igm) antibodies to f. nucleatum, bacteroides gingivalis, actinomyces viscosus, and streptococcus sanguis. antibodies to these four bacteria were detected in cultures stimulated with polyclonal b-cell activatio ...19836604024
phenylacetic acid production by bacteroides gingivalis from phenylalanine and phenylalanine-containing peptides.phenylacetic acid production and growth of bacteroides gingivalis were directly proportional to the trypticase content of the medium. l-phenylalanine enhanced phenylacetic acid production; 5 mg l-phenylalanine per millilitre stimulated maximum production of phenylacetic acid. peptides (2-4 amino acids) containing l-phenylalanine also stimulated phenylacetic acid production as did phenylpyruvic acid. resting cell suspensions of b. gingivalis also produced phenylacetic acid when incubated aerobica ...19836652581
[studies of mixed anaerobic infections involving bacteroides gingivalis].the infectiousness of several combinations of bacteria containing bacteroides gingivalis and other bacteria associated with periodontal diseases was evaluated by subcutaneous injection to guinea pigs. among the seven mixtures studied, only one permitted the development of an infection easily transmissible to a second guinea pig; this bacterial mixture was composed of b. gingivalis, fusobacterium nucleatum, eubacterium saburreum, and capnocytophaga ochracea (formerly bacteroides ochraceus). owing ...19836883221
comparative hydrophobicities of oral bacteria and their adherence to salivary pellicles.oral bacteria were found to differ in their surface hydrophobicities as determined by their ability to adsorb to hexadecane. strains of actinomyces viscosus, a. naeslundii, streptococcus sanguis, s. mitis, and bacteroides gingivalis proved highly hydrophobic. strains of b. intermedius, s. salivarius, s. mutans, and b. melaninogenicus were less hydrophobic, whereas strains of actinobacillus actinomycetemcomitans were hydrophilic. an overall correlation was noted between the adsorption of bacteria ...19836885158
production of phenylacetic acid by bacteroides melaninogenicus ssp. intermedius serogroup c-1.the methyl derivatives of broth cultures of black-pigmented bacteroides were examined by gas chromatography for production of phenylacetic acid. two serogroups of b. melaninogenicus ssp. intermedius described by lambe differed in the ability to produce phenylacetate. serogroup c failed to produce phenylacetic acid while serogroup c-1 produced small amounts of phenylacetate, which contributed 2.2-5.7% to the total nonvolatile acid profile. holdeman's newly proposed species "b. intermedius" and "b ...19836133607
effect of anaerobic bacteria on killing of proteus mirabilis by human polymorphonuclear leukocytes.killing of proteus mirabilis by human polymorphonuclear leukocytes was tested in the presence of different bacteroides species. in vitro experiments showed that anaerobic bacteria interfered with the killing of aerobic bacteria. however, this inhibitory effect was not a property of all bacteroides species. bacteroides gingivalis w83 showed the greatest inhibitory effect of the five bacteroides strains tested. killing of p. mirabilis was inhibited by the culture supernatant of b. gingivalis but n ...19836133837
biological effects of a purified lipopolysaccharide from bacteroides gingivalis. 19836222173
adult respiratory distress syndrome. a need for comparative studies. 1983348141
gilles de la tourette's syndrome: clinical, genetic, psychologic, and biochemical aspects in 21 selected families.eighty-one patients and relatives with tourette's syndrome, members of 21 selected families, participated in a 1-day clinic. in 12 of the 13 jewish families and six of the eight non-jewish families, there were multiple members with motor and vocal tics by observation or history. males predominated among those with persistent symptoms, but among those with spontaneous clearing, females predominated. twelve propositi had troublesome sexual and aggressive impulses, differing only quantitatively fro ...1983264605
effects of an immunomodulating agent on peripheral blood lymphocytes and subgingival microflora in ligature-induced periodontitis.the peripheral blood lymphocyte populations and the subgingival levels of black-pigmented bacteroides species were monitored during the conversion of chronic gingivitis to progressing periodontitis in cynomolgus monkeys. in addition, the effects of an immunomodulating agent, the pentapeptide of thymopoietin (tp5), were determined. after the induction of active periodontitis, proportions of helper t-cells (okt-4 positive) decreased from 31.5 to 26.4%, and proportions of b-cells (surface immunoglo ...19846234232
thiol-dependent collagenolytic activity in culture media of bacteroides gingivalis. 19846235351
proteoglycan production by chick embryonic chondrocytes is inhibited by culture filtrate of bacteroides gingivalis. 19846238146
effects of the association of conventional rats with actinomyces viscosus nyl and bacteroides gingivalis w83. 19846241229
morphological changes in the bacterium bacteroides melaninogenicus subspecies melaninogenicus isolated from the human mouth and grown in culture without added blood components.striking polymorphism in the cellular morphology could be induced by removal of blood components from the liquid growth medium, but the cells of bacteroides gingivalis and b. melaninogenicus subspecies intermedius did not exhibit polymorphism when grown under these conditions. the major changes observed with light microscopy were an increase in cell size and extreme polymorphism. electron microscopy of the polymorphic forms of b. melaninogenicus subspecies melaninogenicus strains showed that suc ...19846140911
purification and characterization of a novel type of fimbriae from the oral anaerobe bacteroides gingivalis.fimbriae and their constituent protein (fimbrilin) were purified to homogeneity from the bacterial wash fluid and cell lysate fraction, respectively, of bacteroides gingivalis 381. fimbriae, observed by negative staining, were curly, single-stranded filaments with a diameter of ca. 5 nm. the apparent molecular weight of the fimbrilin was 43,000. fimbriae were resistant to sodium dodecyl sulfate denaturation at 70 degrees c. heating at 100 degrees c in sodium dodecyl sulfate was needed to complet ...19846150029
detection and quantitation of bacteroides gingivalis in bacterial mixtures by means of flow cytometry. 19846084706
characterization of a trypsin-like protease from the bacterium bacteroides gingivalis isolated from human dental plaque.a trypsin-like, membrane-bound protease from bacteroides gingivalis was solubilized by triton x-100 and partially purified by a combination of deae-sepharose and aminophenylmercuric sepharose chromatography, by taking advantage of the thiol group on the enzyme. the purified enzyme hydrolysed the synthetic substrates benzoyl-l-arginine-p-nitroanilide (l-bapa), benzoyl-d,l-arginine-beta-naphthylamide (bana) and tosyl-l-arginine methyl ester, as well as bovine serum albumin and ovalbumin, but not t ...19846089721
immunogold electron microscopy of surface antigens of oral bacteria.colloidal gold particles 3-6 nm in diameter were prepared and stabilized with the igg fraction of polyspecific rabbit antisera produced against four different oral bacteria. the immunogold markers were used in homologous reactions to label the bacteria in a preembedding procedure for electron microscopy. an indirect immunofluorescence procedure was concurrently used to optimize the labelling conditions before observation with the electron microscope. the immunogold markers labelled fibrillar str ...19846093974
degradation of the human proteinase inhibitors alpha-1-antitrypsin and alpha-2-macroglobulin by bacteroides gingivalis.various strains of black-pigmented bacteroides species were grown on horse blood agar and suspended in human serum. after various times of incubation the effect of the bacteria on the serum was evaluated by polyacrylamide gel electrophoresis and "rocket" immunoelectrophoresis. the formation of trichloroacetic acid-soluble material in the suspensions and the capacity of the treated sera to inhibit the activity of trypsin were also determined. the two tested strains of bacteroides gingivalis (w83, ...19846198282
osteomyelitis in dogs and cats caused by anaerobic bacteria.localised osteomyelitis was diagnosed in 2 dogs and 2 cats. the disease was caused by fight wounds in 3 cases. radiographic examination demonstrated a circumscribed zone of cortical bone lysis, sequestra and periosteal new bone. each case was treated surgically by sequestrectomy and debridement. infection was due mainly to anaerobic bacteria. the pathogenic bacteria isolated from the lesions of dogs were actinomyces viscosus, fusobacterium nucleatum and bacteroides spp, and from the lesions in c ...19846732670
immunoglobulin g response to subgingival gram-negative bacteria in human subjects.serum and gingival crevicular fluid from normal healthy adults and patients with periodontitis were screened for immunoglobulin g antibodies to antigens from bacteroides gingivalis 381, bacteroides intermedius 24, bacteroides loescheii atcc 15930, fusobacterium nucleatum atcc 25586, eikenella corrodens 1073, actinobacillus actinomycetemcomitans atcc 29522, and capnocytophaga sp. strain m-12. immunoglobulin g antibody titers to the antigens were measured by an enzyme-linked immunosorbent assay. t ...19846735473
serological identification of oral bacteroides spp. by enzyme-linked immunosorbent assay.a rapid method for identifying black-pigmented oral bacteroides spp. is described. species-specific rabbit antisera to bacteroides gingivalis, b. intermedius, and b. melaninogenicus were used in an enzyme-linked immunosorbent assay to identify clinical isolates of black-pigmented bacteroides spp. from humans. the results showed excellent agreement with biochemical identification of b. gingivalis and b. intermedius. only 36% of the b. melaninogenicus isolates were identified with the enzyme-linke ...19846736225
fractionation of hemagglutinating and bacterial binding adhesins of bacteroides gingivalis.an outer membrane complex containing hemagglutinating and bacterial aggregating activity has been isolated from bacteroides gingivalis. examination of the membrane material by biochemical analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunological means revealed that the crude outer membrane preparation contained three major proteins and a lipopolysaccharide population that displayed size heterogeneity. at least two membrane proteins as well as the lipopolysaccharide w ...19846746097
colonization of the subgingival area by bacteroides gingivalis.the initial event in colonization of the subgingival area by b. gingivalis is its attachment to host cells and gram-positive bacteria in pre-formed plaque. the level of b. gingivalis is partly governed by products of other plaque bacteria, especially by sanguicin. once b. gingivalis resides in its nidus and starts to proliferate, expulsion of pre-existing residents may occur, especially of attached gram-positive bacteria, through the inhibitory action of the b. gingivalis product, hematin. the b ...19846583244
[susceptibility of potential periodontopathic bacteria to metronidazole, spiramycin and their combination].a total of 65 bacterial strains originating mostly from subgingival plaque were tested for their susceptibilities to metronidazole, spiramycin, and their combination, ornidazole, erythromycin and tetracycline by means of an agar dilution technique. all agents were active against all anaerobic gram-negative rods. bacteroides gingivalis and fusobacterium nucleatum showed marked susceptibility to metronidazole (mic less than or equal to 0.06 microgram/ml) whereas 4-64 micrograms/ml were required to ...19846584423
[experimental periodontitis in hamsters induced by infection with bacteroides gingivalis]. 19846591433
immunologic profile of juvenile periodontitis. i. lymphocyte blastogenesis and the autologous mixed lymphocyte response.studies of blastogenesis of lymphocytes in culture from juvenile periodontitis (jp) patients have been inconclusive. experiments demonstrating differences in lymphocyte blastogenesis to preparations of putative periodontopathogens in jp and phytohaemagglutinin (pha) were performed. variables in the blastogenesis assay system recently reported were controlled which included using a range of cell and activator concentrations, incubation times of 3, 5 and 7 days, conical-bottomed microtest wells an ...19846592318
[isolation and identification of bacteroides gingivalis in rapidly progressing periodontitis in koreans]. 19846596344
the bone-resorbing activities in tissue culture of lipopolysaccharides from the bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis and capnocytophaga ochracea isolated from human mouths.the activities of lipopolysaccharides (lps) were assessed by measuring the calcium release from mouse calvaria in vitro and compared to that of lps from salmonella typhimurium. stimulation of bone resorption was maximal at an lps concentration of 10 micrograms/ml and at this dose all oral lps preparations showed similar levels of activity and less than that of lps from s. typhimurium. only s. typhimurium lps and b. gingivalis lps retained bone-resorbing activity at 0.1 microgram/ml. no bone-reso ...19846362631
degradation in vivo of the c3 protein of guinea-pig complement by a pathogenic strain of bacteroides gingivalis.the pathogenicity of five black-pigmented strains of bacteroides was tested in subcutaneously implanted teflon cages in guinea pigs. the tissue reaction around the cages was registered and the contents of the fluid of the cages were analyzed. two strains of b. intermedius produced a localized abscess around the cages, while one strain (381) of b. gingivalis and an asaccharolytic strain (bn11a-f) different from b. gingivalis did not induce any signs of abscess formation. one strain (w83) of b. gi ...19846372060
metronidazole in periodontitis. i. clinical and bacteriological results after 15 to 30 weeks.the statistical association of certain anaerobic organisms such as black pigmented bacteroides (bpb) species and spirochetes with clinical signs of active periodontitis, i.e. bleeding upon probing and bone loss, suggests that the lesions may actually reflect a "specific infection" involving these or unidentified species. all the known oral species of bpb and spirochetes are anaerobes which suggests that antimicrobial treatment directed specifically against anaerobes might be effective in periodo ...19846376759
identification of bacteria in immunopathologic mechanisms of human dental pulp.serial frozen sections from human dental pulp were used for the identification of oral bacteria in immunopathologic mechanisms. sera raised against actinomyces viscosus, a. naeslundii, bacteroides gingivalis, and b. melaninogenicus ss. intermedius, commercial sera against human immunoglobulins, complement, and monoclonal antibodies against human t cells were used in a double-staining immunofluorescence technique. sections of dental pulp from normal teeth showed no penetration of bacteria or bact ...19846377171
actinobacillus actinomycetemcomitans and bacteroides gingivalis in advanced periodontitis in man. 19846386438
ability of various oral bacteria to bind human plasma fibronectin.the present study describes the ability of various oral bacteria to bind human plasma fibronectin (pfn). avid binding of 125i-pfn was found for streptococcus mutans (serotypes a to h), streptococcus sanguis, group a streptococcus pyogenes and staphylococcus aureus, while other gram-positive bacterial species tested demonstrated only weak or negligible pfn binding ability. two gram-negative bacterial species, bacteroides gingivalis and escherichia coli, did not significantly bind pfn. 125i-pfn bi ...19846390098
production of monoclonal antibodies that recognize specific and cross-reactive antigens of bacteroides gingivalis.four monoclonal antibodies directed against bacteroides gingivalis were established by hybridoma technology. their reactivity against b. gingivalis, bacteroides intermedius, and bacteroides melaninogenicus was detected by the enzyme-linked immunosorbent assay. three monoclonal antibodies specifically reacted with b. gingivalis. one recognized antigens that were cross-reactive between b. gingivalis and b. intermedius. these monoclonal antibodies provide new tools for antigenic analysis of b. ging ...19846480112
planning and evaluation: the roles of the public and voluntary sectors. 1984263260
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