Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
case of xanthogranulomatous oophoritis.xanthogranulomatous inflammation is characterized by destruction of the tissues of the organ involved and replacement by chronic inflammatory cells such as lymphocytes, plasma cells, occasional neutrophils with or without multinucleated or touton giant cells. exact aetiology is not known but the theory of infection with organisms like proteus, e coli, and bacteroides fragilis is most popular. xanthogranulomatous inflammation of the female genital tract is not common and usually involves the endo ...201728712201
effect of soy isoflavones on growth of representative bacterial species from the human gut.the present work aimed to assess the susceptibility of dominant and representative bacterial populations from the human gut to isoflavones and their metabolites. to do so, the minimum inhibitory concentration (mic) of isoflavone glycosides, isoflavone aglycones, and equol to 37 bacterial strains was determined by broth microdilution. additionally, for 10 representative strains, growth curves, growth rate (μ), and optical density (od600 nm) of the cultures at 24 h were also determined. mics of da ...201728698467
outer membrane vesicles secreted by pathogenic and nonpathogenic bacteroides fragilis represent different metabolic activities.numerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. in this regard, vesicles secreted by bacteria represent one of the most popular topics for research. for example, the outer membrane vesicles (omvs) of bacteroides fragilis play an important nutritional role with respect to other microorganisms and promote anti-inflammatory effects on immune cells. however, toxigenic b. fragilis (etbf) contributes to bowel disease, even causing colo ...201728694488
the bacteroides fragilis pathogenicity island links virulence and strain competition.the mature microbiome is a stable ecosystem that resists perturbation despite constant host exposure to exogenous microbes. however, the microbial mechanisms determining microbiome development and composition are poorly understood. we recently demonstrated that a non-toxigenic b. fragilis (ntbf) strain restricts enteric colonization by an enterotoxigenic (etbf) strain dependent on a type vi secretion system (t6ss). we show here that a second enterotoxigenic strain is competent to colonize, depen ...201728632016
septic abortion presenting as a right lower trapezius abscess secondary to bacteroides fragilis bacteraemia.a 43-year-old japanese woman was evaluated in the outpatient department for right shoulder pain and fever, which began 5 days earlier. mri of the right shoulder revealed a high-intensity area deep in the right trapezius muscle. aspiration revealed purulent fluid, and gram staining of the fluid showed gram-negative bacilli. the patient was also found to be profoundly anaemic and to have a positive urine pregnancy test. on admission, we initiated intravenous ampicillin-sulbactam and aztreonam. she ...201728827296
in vitro/ in vivo evaluation of the efficacy of gatifloxacine-loaded plga and hydroxyapatite composite for treating osteomyelitis.molten 10 wt% gatifloxacine (glfx-loaded poly(lactide-co-glycolide) (plga) was introduced into three-dimensionally interconnected pores and onto the surfaces of hydroxyapatite (ha) granules. the composite granules exhibited clinically sufficient bactericidal activities against streptococcus milleri and bacteroides fragilis from 3 h to 10 days. the composite granules were implanted in bone defects created by debridement of osteomyelitis lesions in rabbit mandibles. after 4-week implantation, infl ...201728626206
the effects of the lactobacillus casei strain on obesity in children: a pilot study.there are few data regarding the role of probiotics as a dietary intervention in the management of obesity in children. an open prospective examination was conducted to clarify the effects of lactobacillus casei strain shirota (lcs)-containing beverages in obese children. we compared the intestinal microbiota and organic acid levels between 12 obese (average age, 10.8 years; body mass index (bmi) z score, 2.7±1.7) and 22 control children(average age, 8.5 years; bmi z score, 0.1±0.7), and pre- an ...201728618860
antimicrobial susceptibility of bacteroides fragilis group organisms in hong kong by the tentative eucast disc diffusion method.this study used a recently developed eucast disc diffusion method to measure the susceptibility of 741 b. fragilis group isolates to six antibiotics. isolates nonsusceptible to imipenem and metronidazole by the disc method were further investigated by e-test. species identification was obtained by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms), pcr assays and 16s rrna sequencing. the most common species were b. fragilis (n = 424, including 81 division ...201728414107
inactivation of marr gene homologs increases susceptibility to antimicrobials in bacteroides fragilis.bacteroides fragilis is the strict anaerobic bacteria most commonly found in human infections, and has a high mortality rate. among other virulence factors, the remarkable ability to acquire resistance to a variety of antimicrobial agents and to tolerate nanomolar concentrations of oxygen explains in part their success in causing infection and colonizing the mucosa. much attention has been given to genes related to multiple drug resistance derived from plasmids, integrons or transposon, but such ...201728847541
nim gene-independent metronidazole-resistant bacteroides fragilis in surgical site infections.background:bacteroides fragilis is the most common anaerobic pathogen isolated from surgical site infections (ssis). metronidazole resistance is increasing and the mechanisms of resistance are not clear in some isolates. the aim of the present study was to investigate the metronidazole susceptibility prevalence, and detect nim genes in b. fragilis isolates from ssis. methods: this study included 100 surgery patients with signs and symptoms indicative of ssis. syringe aspiration of the infected s ...201728840093
tlr2-stimulating contaminants in glycoconjugate fractions prepared from bacteroides fragilis.bacteroides fragilis is a member of the normal intestinal flora and is involved in host immunostimulation via tlr2. on the bacterial cell surface, glycoconjugates, such as lps and capsular polysaccharide a (psa), have been reported to participate in host immunostimulation via tlr2. previously, we identified a tlr2-stimulating lipoprotein in b. fragilis cells. in this study, we demonstrated that tlr2-stimulating principal molecules in glycoconjugate fractions prepared from b. fragilis are contami ...201728606014
implementation of the new virtuo blood culture system: evaluation and comparison to the 3d system using simulated blood cultures.to evaluate the performances of the newly approved bact/alert virtuo blood culture system for the recovery of bloodstream pathogens and compare it to the bact/alert 3d system.201728583022
bioluminescence imaging to track bacteroides fragilis inhibition of vibrio parahaemolyticus infection in mice.bacteroides fragilis is an anaerobic, gram-negative, commensal bacterium of the human gut. it plays an important role in promoting the maturation of the immune system, as well as suppressing abnormal inflammation. many recent studies have focused on the relationship between b. fragilis and human immunity, and indicate that b. fragilis has many useful probiotic effects. as inhibition of intestinal pathogens is an important characteristic of probiotic strains, this study examined whether b. fragil ...201728553617
[the effect of oxygen on endotoxin production in bacteria of the bacteroides fragilis group isolated from patients with colorectal carcinoma].the aim of the study was to draw attention to the risk posed by anaerobic bacteria of the bacteroides fragilis (bafr) group, isolated particularly from abdominal lesions, and to assess the possible role of these species in colorectal cancer. a correlation has previously been suggested between the detection of the bacteria of the genus bacteroides in patients on a meat-based diet and intestinal and, in particular, colorectal cancer. given that the species of the bafr group are major producers of ...201727467330
gut microbiota profiling of pediatric nonalcoholic fatty liver disease and obese patients unveiled by an integrated meta-omics-based approach.there is evidence that nonalcoholic fatty liver disease (nafld) is affected by gut microbiota. therefore, we investigated its modifications in pediatric nafld patients using targeted metagenomics and metabolomics. stools were collected from 61 consecutive patients diagnosed with nonalcoholic fatty liver (nafl), nonalcoholic steatohepatitis (nash), or obesity and 54 healthy controls (ctrls), matched in a case-control fashion. operational taxonomic units were pyrosequenced targeting 16s ribosomal ...201727028797
the underappreciated in vitro activity of tedizolid against bacteroides fragilis species, including strains resistant to metronidazole and carbapenems.because bacteroides fragilis has the ability to develop mechanisms of resistance to almost all antibiotics, we studied the comparative in vitro activity of tedizolid against 124 bacteroides group species clinical isolates, including carbapenem, metronidazole and piperacillin-tazobactam resistant strains. tedizolid had an mic90 of 2 μg/ml (range, 0.5-4 μg/ml) and was 1-4 times more active than linezolid that had an mic90 of 8 μg/ml (range, 2-16 μg/ml). it was also active (mics 0.5-2 μg/ml) agains ...201727713022
structural and functional analysis of bf2549, a padr-like transcription factor from bacteroides fragilis.a phenolic acid decarboxylase (padc) regulator, padr and its homologs proteins belong to the padr family. despite the growing numbers of the padr family members and their various roles in bacteria, such as detoxifications, drug transports and circadian rhythms, biochemical and biophysical studies of the padr family are very limited. thus, a ligand-induced regulatory mechanism of the padr family transcription factors remains to be elucidated. here, we report a crystal structure of a bacteroides f ...201728027933
phenotypic detection of the cfia metallo-β-lactamase in bacteroides fragilis with the meropenem-edta double-ended etest and the rosco kpc/mbl confirm kit.to investigate the performance of the meropenem and imipenem double-ended etest ± edta and the tablet-based (meropenem and meropenem + dipicolinic acid) kpc/mbl confirm kit to detect cfia metallo-β-lactamase (mbl) in bacteroides fragilis.201727798215
enterotoxigenic and non-enterotoxigenic bacteroides fragilis from fecal microbiota of children.enterotoxigenic bacteroides fragilis (etbf) is an important part of the human and animal intestinal microbiota and is commonly associated with diarrhea. etbf strains produce an enterotoxin encoded by the bft gene located in the b. fragilis pathogenicity island (bfpai). non-enterotoxigenic b. fragilis (ntbf) strains lack the bfpai and usually show two different genetic patterns, ii and iii, based on the absence or presence of a bfpai-flanking region, respectively. the incidence of etbf and ntbf s ...201726691473
the myeloid immune signature of enterotoxigenic bacteroides fragilis-induced murine colon tumorigenesis.enterotoxigenic bacteroides fragilis (etbf), a human commensal and candidate pathogen in colorectal cancer (crc), is a potent initiator of interleukin-17 (il-17)-dependent colon tumorigenesis in min(apc+/-) mice. we examined the role of il-17 and etbf on the differentiation of myeloid cells into myeloid-derived suppressor cells (mdscs) and tumor-associated macrophages, which are known to promote tumorigenesis. the myeloid compartment associated with etbf-induced colon tumorigenesis in min mice w ...201727301879
mycotic aneurysm caused by bacteroides fragilis in an elderly immunosuppressed patient.an 82-year-old japanese man, who presented with a fever and abdominal pain, was admitted to our hospital. according to enhanced computed tomography images, the probable diagnosis was abdominal aortic mycotic aneurysm. eight sets of blood cultures obtained from the patient were negative. despite administering treatment with vancomycin and ceftriaxone, the aneurysm progressively enlarged. he underwent open debridement surgery and in situ replacement because of an aneurysmal rupture. bacteroides fr ...201727904124
clinical characteristics and antimicrobial susceptibilities of anaerobic bacteremia in an acute care hospital.this study investigated the clinical features of anaerobic bacteraemia in an acute-care hospital, and evaluated the antimicrobial susceptibility of these isolates to commonly available antibiotics. microbiological and epidemiological data from 2009 to 2011were extracted from the laboratory information system and electronic medical records. one hundred and eleven unique patient episodes consisting of 116 anaerobic isolates were selected for clinical review and antibiotic susceptibility testing. s ...201727890724
trends in antimicrobial resistance among bacteroides species and parabacteroides species in the united states from 2010-2012 with comparison to 2008-2009.the susceptibility trends for bacteroides fragilis and related species against various antibiotics were determined using data from 3 years of surveillance (2010-2012) on 779 isolates referred by 7 medical centers. the antibiotic test panel included imipenem, ertapenem, meropenem, ampicillin-sulbactam, piperacillin-tazobactam, cefoxitin, clindamycin, moxifloxacin, tigecycline, linezolid, chloramphenicol and . mics were determined using the agar dilution clsi reference method. carbapenem resistanc ...201727867083
a novel selective medium for isolation of bacteroides fragilis from clinical specimens.a novel bacteroides fragilis selective (bfs) medium, consisting of a brain heart infusion agar base supplemented with yeast extract, cysteine hydrochloride, bile salts, vitamin k, hemin, glucose, esculin, ferric ammonium citrate, bromothymol blue, gentamicin, kanamycin, and novobiocin, was evaluated. when bfs agar was tested with a collection of 303 bacteria of different genera, it allowed the growth of b. fragilis as large yellow colonies, with blackening of the medium after 48 h of anaerobic i ...201727852672
association of brain amyloidosis with pro-inflammatory gut bacterial taxa and peripheral inflammation markers in cognitively impaired elderly.the pathway leading from amyloid-β deposition to cognitive impairment is believed to be a cornerstone of the pathogenesis of alzheimer's disease (ad). however, what drives amyloid buildup in sporadic nongenetic cases of ad is still unknown. ad brains feature an inflammatory reaction around amyloid plaques, and a specific subset of the gut microbiota (gmb) may promote brain inflammation. we investigated the possible role of the gmb in ad pathogenesis by studying the association of brain amyloidos ...201727776263
colorectal carcinoma: a general overview and future perspectives in colorectal cancer.colorectal cancer (crc) is the third most common cancer and the fourth most common cause of cancer-related death. most cases of crc are detected in western countries, with its incidence increasing year by year. the probability of suffering from colorectal cancer is about 4%-5% and the risk for developing crc is associated with personal features or habits such as age, chronic disease history and lifestyle. in this context, the gut microbiota has a relevant role, and dysbiosis situations can induc ...201728106826
complete tetrasaccharide repeat unit biosynthesis of the immunomodulatory bacteroides fragilis capsular polysaccharide a.capsular polysaccharide a (cpsa) is a four-sugar repeating unit polymer found on the surface of the gut symbiont bacteroides fragilis that has therapeutic potential in animal models of autoimmune disorders. this therapeutic potential has been credited to its zwitterionic character derived from a positively charged n-acetyl-4-aminogalactosamine (aadgal) and a negatively charged 4,6-o-pyruvylated galactose (pyrgal). in this report, using a fluorescent polyisoprenoid chemical probe, the complete en ...201728103676
modeling diversity in structures of bacterial outer membrane lipids.lipopolysaccharides (lpss) are vital components of the outer membrane of gram-negative bacteria, and they act as extremely strong stimulators of innate immunity in diverse eukaryotic species. the primary immunostimulatory center of the lps molecule is lipid a, a disaccharide-bound lipophilic domain. considering the broad diversity in bacterial species, there are variations in the lipid a structures and their immunogenic potencies. in this work, we model the lipid a structures of eight commensal ...201728080049
respiration of microbiota-derived 1,2-propanediol drives salmonella expansion during colitis.intestinal inflammation caused by salmonella enterica serovar typhimurium increases the availability of electron acceptors that fuel a respiratory growth of the pathogen in the intestinal lumen. here we show that one of the carbon sources driving this respiratory expansion in the mouse model is 1,2-propanediol, a microbial fermentation product. 1,2-propanediol utilization required intestinal inflammation induced by virulence factors of the pathogen. s. typhimurium used both aerobic and anaerobic ...201728056091
collateral damage: insights into bacterial mechanisms that predispose host cells to cancer.infections are estimated to contribute to 20% of all human tumours. viruses are known to induce cell transformation, but evidence has also linked bacteria, such as helicobacter pylori and salmonella enterica subsp. enterica serovar typhi, to different cancer types. in addition, chlamydia trachomatis, fusobacterium nucleatum and bacteroides fragilis are associated with the development of cancer, although a causal relationship has not yet been established. bacterial effectors such as colibactin an ...201728045107
an active principle of nigella sativa l., thymoquinone, showing significant antimicrobial activity against anaerobic bacteria.thymoquinone (tq) is the major active principle of nigella sativa seed (black seed) and is known to control many fungi, bacteria, and some viruses. however, the activity of tq against anaerobic bacteria is not well demonstrated. anaerobic bacteria can cause severe infections, including diarrhea, aspiration pneumonia, and brain abscess, particularly in immunodeficient individuals. the present study aimed to investigate the in vitro antimicrobial activity of tq against some anaerobic pathogens in ...201728163966
we are not alone: a case for the human microbiome in extra intestinal diseases."dysbiosis" in the gut microbiome has been implicated in auto-immune diseases, in inflammatory diseases, in some cancers and mental disorders. the challenge is to unravel the cellular and molecular basis of dysbiosis so as to understand the disease manifestation.201728286571
antimicrobial resistance in the bacteroides fragilis group in faecal microbiota from healthy danish children.the bacteroides fragilis group constitute a significant portion of the human gut microbiota and comprise a major proportion of anaerobic bacteria isolated in human infections. we established a baseline of antimicrobial susceptibility rates in the b. fragilis group in the intestinal tract of relatively antibiotic-naive healthy danish children. from 174 faecal samples collected from children attending day care, 359 non-duplicate isolates were screened for antimicrobial susceptibility. of these, 0. ...201728366659
the symbiotic bacterial surface factor polysaccharide a on bacteroides fragilis inhibits il-1β-induced inflammation in human fetal enterocytes via toll receptors 2 and 4.colonizing bacteria interacting with the immature, unlike the mature, human intestine favors inflammation over immune homeostasis. as a result, ten percent of premature infants under 1500 grams weight develop an inflammatory necrosis of the intestine after birth, e.g., necrotizing enterocolitis (nec). nec is a major health problem in this population causing extensive morbidity and mortality and an enormous expenditure of health care dollars. nec can be prevented by giving preterm infants their m ...201728278201
monoclonal antibodies specific for bacteroides fragilis enterotoxins bft1 and bft2 and their use in immunoassays.we have developed 22 mouse igg1 monoclonal antibodies (mabs) against bacteroides fragilis zinc metalloprotease toxins 1 and 2 (bft1 and bft2). mice were immunized with recombinant bft1 or bft2 proteins with metalloprotease activity. eight of the mabs bind specifically to bft1. one mab, 2h6, binds specifically to bft2. the remaining 13 mabs bind to both bft1 and bft2. the eight bft1-specific mabs recognize at least five different epitopes on the toxin. four of the bft1-specific mabs neutralized r ...201728257448
fatal sepsis caused by multidrug-resistant bacteroides fragilis, harboring a cfia gene and an upstream insertion sequence element, in japan.here, we report a case of fatal sepsis resulting from an intra-abdominal infection caused by a bacteroides fragilis strain containing a cfia4 metallo-β-lactamase and an upstream insertion sequence (is) element. meropenem was used as empiric therapy for septic shock as a result of the intra-abdominal infection, although two rounds of carbapenem treatment had been administered previously. b. fragilis was isolated from two anaerobic blood culture bottles 4 days after the onset of septic shock. susc ...201728108390
diastereomer-specific quantification of bioactive hexosylceramides from bacteria and mammals.mammals synthesize cell-type specifically the diastereomeric hexosylceramides β-galactosylceramide (β-galcer) and β-glucosylceramide (β-glccer), which are involved in several diseases such as sphingolipidosis, diabetes, chronic kidney diseases, or cancer. in contrast bacteroides fragilis, a member of the human gut microbiome, and the marine sponge agelas mauritianus produce α-galactosylceramide (α-galcer), one of the most potent stimulators for inkt cells. to dissect the contribution of these in ...201728373486
safety evaluation of a novel strain of bacteroides fragilis.commensal non-toxigenic bacteroides fragilis confers powerful health benefits to the host, and has recently been identified as a promising probiotic candidate. we previously isolated b. fragilis strain zy-312 and identified it as a novel strain based on 16s rrna sequencing and morphological analyses. we also determined that zy-312 displayed desirable probiotic properties, including tolerance to simulated digestive fluid, adherence, and in vitro safety. in this study, we aim to investigate whethe ...201728367145
biofilm colonization in chronic treatment refractory infections presenting with discharging sinuses: a study in a tertiary care hospital of eastern india.treatment refractory chronic recurrent infections mean those chronic infections which recur by same causal agents with similar drug responsiveness after apparent relief following full course of recommended antimicrobial management.201728367029
interaction of bacteroides fragilis toxin with outer membrane vesicles reveals new mechanism of its secretion and delivery.the only recognized virulence factor of enterotoxigenic bacteroides fragilis (etbf) that accompanies bloodstream infections is the zinc-dependent non-lethal metalloprotease b. fragilis toxin (bft). the isolated toxin stimulates intestinal secretion, resulting in epithelial damage and necrosis. numerous publications have focused on the interrelation of bft with intestinal inflammation and colorectal neoplasia, but nothing is known about the mechanism of its secretion and delivery to host cells. h ...201728144586
rapid detection of cfia metallo-β-lactamase-producing bacteroides fragilis by the combination of maldi-tof ms and carbanp.carbapenem resistance in bacteroides fragilis is emerging and is mainly attributed to insertion sequence (is)-mediated activation of the carbapenemase gene cfia. we investigated the use of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (maldi-tof ms) and the carbanp assay for the rapid identification of these strains.201728360188
branimycins b and c, antibiotics produced by the abyssal actinobacterium pseudonocardia carboxydivorans m-227.two new antibiotics, branimycins b (2) and c (3), were produced by fermentation of the abyssal actinobacterium pseudonocardia carboxydivorans m-227, isolated from deep seawater of the avilés submarine canyon. their structures were elucidated by hrms and nmr analyses. these compounds exhibit antibacterial activities against a panel of gram-positive bacteria, including corynebacterium urealyticum, clostridium perfringens, and micrococcus luteus, and against the gram-negative bacterium neisseria me ...201728169531
assessment of swine-specific bacteriophages of bacteroides fragilis in swine farms with different antibiotic practices.we assessed the occurrence and specificity of bacteriophages of bacteroides fragilis in swine farms for their potential application in microbial source tracking. a local b. fragilis host strain, sp25 (dsm29413), was isolated from a pooled swine feces sample taken from a non-antibiotic farm. this strain was highly specific to swine fecal materials because it did not detect bacteriophages in any samples from human sewage, sheep, goats, cattle, dogs, and cats. the reference b. fragilis strain, ryc2 ...201728362306
in vitro anaerobic pharmacokinetic/pharmacodynamic model to simulate the bactericidal activity of levornidazole against bacteroides fragilis.this study was designed to correlate the pharmacokinetic/pharmacodynamic (pk/pd) parameters with pd indices of levornidazole against bacteroides fragilis and to calculate the pk/pd target value for levornidazole to attain its expected maximal bactericidal effect using an in vitro anaerobic dynamic pk/pd model.201728363695
colonization with enterotoxigenic bacteroides fragilis is associated with early-stage colorectal neoplasia.enterotoxigenic bacteroides fragilis (etbf) is a toxin-producing bacteria thought to possibly promote colorectal carcinogenesis by modulating the mucosal immune response and inducing epithelial cell changes. here, we aim to examine the association of colonic mucosal colonization with etbf and the presence of a range of lesions on the colonic neoplastic spectrum.201728151975
suppression of colorectal tumorigenesis by recombinant bacteroides fragilis enterotoxin-2 in vivo.to evaluate the impact of recombinant bacteroides fragilis enterotoxin-2 (bft-2, or fragilysin) on colorectal tumorigenesis in mice induced by azoxymethane/dextran sulfate sodium (aom/dss).201728216966
cystic fibrosis transmembrane conductance regulator knockout mice exhibit aberrant gastrointestinal microbiota.the composition of the gastrointestinal microbiome is increasingly recognized as a crucial contributor to immune and metabolic homeostasis-deficiencies in which are characteristic of cystic fibrosis (cf) patients. the murine model (cftr (-/-) , cf), has, in previous studies, demonstrated characteristic cf gastrointestinal (gi) manifestations including slowed transit and significant upregulation of genes associated with inflammation. to determine if characteristics of the microbiome are associate ...201723060053
Refresh
firstprev
...101
nextlast
Displaying items 10001 - 10047 of 10047