Publications

reduction of ferricytochrome c, methemoglobin and metmyoglobin by hydroxyl and alcohol radicals.	we have studied the reaction of ferricytochrome c, methemoglobin and metmyoglobin with oh and alcohol radicals (methanol, ethanol, ethylene glycol and glycerol). these radicals can be divided into three groups: 1. the oh radicals which reduce the ferricytochrome c with a yield of (30 +/- 10)% and methemoglobin with a yield of (40 +/- 10)%. they do not reduce metmyoglobin. the reduction is not a normal bimolecular reaction but is most probably an intramolecular electron transfer of a protein radi ...	1978	222335
3rd industrial and environmental neurology congress. prague 25-28th june 1979 [abstracts].		1979	550690
effect on rats and mice of a diet with low tryptophan content (maize flour).	female wistar rats when crowded together become aggressive if fed a diet with low tryptophan (trp) content (maize, flour, mf). when isolated during 30 days and fed mf they show a decrease of weight, of total plasma trp, and of trp and 5-hydroxytryptamine (5-ht) in brain stem, but 5-hydroxyindolacetic acid (5-hiaa) of the same cerebral area does not change. these results partially agree with other authors observations. mice isolated during 47 days and fed with mf do not show any significant weigh ...	1979	95611
systemic hyalinosis (juvenile hyaline fibromatosis). ultrastructure of the hyaline with particular reference to the cross-banded structure.	systematic hyalinosis (juvenile hyaline fibromatosis) is characterized by hyalinized skin lesions. electron microscopic examination of a hyalinized skin tumor from a 19-year-old man with this syndrome revealed that the hyaline was composed of ruthenium red-positive ultrastructures (granules, filaments, and a kind of cross-banded structure), indicating the presence of glycosamino-glycan or glycoprotein, and a small number of thin collagen fibrils. using a new ruthenium red staining method combine ...	1979	88923
the influenza virus: antigenic composition and immune response.	the architecture and chemical composition of the influenza virus particle is described with particular reference to the protein constituents and their genetic control. the dominant role in infection of the surface proteins - haemagglutinins and neuraminidases - acting as antigens and undergoing variation in time known as antigenic drift and shift is explained. the immuno-diffusion technique has illuminated the interrelationships of the haemagglutinins of influenza a viruses recovered over long p ...	1979	88726
anaphylatoxin-induced shock and two patterns of anaphylactic shock: hemodynamics and mediators.	in the dog, different cardiorespiratory reactions were identified in two types of anaphylactic shock and in c5a-at (anaphylatoxin)-induced shock. all three types had in common a portal blood pooling with consequent decrease in the venous return, cardiac output, and arterial pressure. in anaphylaxis (a) of the first type, at a low titer of hemagglutinating antibodies, the latent period was 68 s and heart and lung function was unchanged. in the second type, at high titer, the latency was 19 s and ...	1979	88165
expression in escherichia coli of chemically synthesized genes for human insulin.	synthetic genes for human insulin a and b chains were cloned separately in plasmid pbr322. the cloned synthetic genes were then fused to an escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. the insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. complete purification of the a chain and partial purification of the b chain were achieved. these products were mixed, reduced, and r ...	1979	85300
in-phase brain activities during arousal and sleep.	eeg of sensorimotor area, emg of neck muscles and unit activity in lateral geniculate (lg) nucleus, recorded in freely moving cats, were analysed for the presence of correlations between them. the 3 activities were first integrated with a 2 sec time constant, then a correlation coefficient was assessed between time-linked points of the 3 records for periods of 40 sec. the average vlaues of the correlation coefficients were found to increase from arousal through slow synchronized sleep (s sleep), ...	1979	89948
growth of rickettsia typhi in irradiated l cells enhanced by lysosomal stabilization.	the growth of some obligate intracellular parasites is contingent upon avoidance of lysosomal activation during growth in their host cells. this is accomplished by the various parasites by different mechanisms and with different degrees of efficiency. the possibility was tested that the lysosomal stabilizer cortisone acetate might protect and thus enhance the growth of rickettsia typhi in mouse l cells irradiated 6 days earlier. beginning 2 days before infection of the l cells with a multiplicit ...	1979	106007
[intravenous cholangiography in hyperbilirubinaemia (author's transl)].	the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ...	1979	153309
mycoplasma pneumonia presenting as meningoencephalitis and hemolytic anemia.	simultaneous occurrence of cold agglutinin hemolytic anemia and central nervous system disease due to mycoplasma pneumonia is extremely rare. a patient is described who initially presented with severe hemolysis and diffuse neurologic dysfunction which appeared during the period of roentgenographic resolution of her pneumonia. spectrum, pathogenesis, and antimicrobial treatment of the neurologic complications are reviewed.	1979	94244
[the mechanism of transcription in procaryotes and eucaryotes (author's transl)].		1979	94670
biochemical effects of the modification of nucleic acids by certain polycyclic aromatic carcinogens.		1979	95052
[lipopolysaccharides of rickettsiaceae and the limulus endotoxin assay].	the a.a. have examined by limulus endotoxin assay some lps preparations from r. typhi, r. slovaka, c. burnetti phase i and ii, as a demonstration of endotoxicity. all the preparations tested were able to gel the limulus amoebocyte lysates, even if to a different degree of reactivity. the results add credibility to the hypothesis that lps from rickettsiaceae can represent at least in part the pathogenetic mediators of some manifestations described in rickettsiosis; however, without endotoxin assa ...	1979	121982
on the life cycle of theileria velifera (uilenberg, 1964) in the gut and the haemolymph of the tick vector amblyomma variegatum (fabriciu, 1794).	the development of theileria velifera in the gut and the haemolymph of the tick vector amblyomma variegatum is described. between days three and eight after repletion various sexual stages are present in the gut of the nymphs. zygotes were detected in the gut on day 14, while undifferentiated kinetes first appeared on day 15. in the haemolymph, differentiated kinetes were observed from day 16 after repletion onwards. zygotes and kinetes were also found after the moult, in the adult tick, for a p ...	1979	120627
isolation and characterization of recombinant dna plasmids carrying drosophila trna genes.	recombinant plasmids carrying drosophila melanogaster trna genes were constructed by ligation of hindiii-cleaved drosophila dna to hindiii cut pbr322 dna. 90 clones were isolated that contained genes for one or more of eleven trnas. 43 of the plasmids were characterized by a number of methods: restriction nuclease digestion; agarose gel electrophoresis; hybridization with individual, purified, 125i-labelled drosophila trna molecules and in situ hybridization to drosophila chromosomes. the result ...	1979	118082
mechanisms of immunity in typhus infection: adoptive transfer of immunity to rickettsia mooseri.	when nonimmune guinea pigs are inoculated intradermally (i.d.) with rickettsia mooseri (r. typhi), the rickettsiae replicate at the site of inoculation, leading to the development of a grossly observable lesion. in contrast, guinea pigs which have recovered from r. mooseri infection are resistant to challenge and prevent both rickettsial growth and the formation of lesions. to study the mechanisms of this immunity, sera or splenic cells collected from nonimmune or immune guinea pigs were inocula ...	1979	110699
isolation of listeria monocytogenes from animals in the sudan.		1979	107996
polyploidy and domestication: the origin and survival of polyploids in cytotype mixtures.	the origin and survival of a polyploid in a mixture of this polyploid and its parent(s) is reviewed. with several examples a picture is drawn of the interference of cytotypes in a mixture of cytotypes. some natural polyploids, both wild and domesticated, are very successful. they, like bread wheat and banana, largely replaced their parents. the same is true for some artificial polyploids like autotriploid hybride sugar beet in europe and autotetraploid perennial ryegrass. but when grown together ...	1979	550834
[effects of low molecular weight nuclear rnas on the activity of rna polymerase ii in isolated rat liver nuclei].	an attempt was made to elucidate the possible participation of low molecular weight nuclear rnas in the transcription process. the parameters of the rna synthesis cell-free system in isolated rat liver nuclei were studied for this purpose. tissue homogenization in isotonic sucrose during isolation of the nuclei resulted in a loss of 4s nuclear rna. the dependence of rna synthesis on the salt concentration in the incubation medium is indicative of possible initiation of the rna synthesis in this ...	1979	420876
antibody to rickettsia mooseri erythrocyte-sensitizing substance.	erythrocyte-sensitizing antigens from rickettsia mooseri (r. typhi) were found to bind to rabbit erythrocytes. upon reintroduction into the donor, such antigen-sensitized cells caused the production of antibodies of restricted serological reactivity.	1979	468383
regulation of the in vitro anamnestic antibody response by cyclic amp. iii. cholera enterotoxin induces lymph node cells to release soluble factor(s) which enhance(s) antibody synthesis by antigen-treated lymph node cells.		1979	228867
diffusion-controlled reactions on spherical surfaces. application to bacteriophage tail fiber attachment.	we have explored the kinetic implications of a model that may account for the acceleration of tail fiber (f) attachment to baseplates (b) by whiskers (w) on bacteriophage t4. the model assumes that a w-f complex is formed initially, and that the tethered fiber then undergoes rotational diffusion until a b-f encounter takes place. in the absence of whiskers, b-f complexes must form unassisted. formation of a w-f intermediate will accelerate f attachment to b if (a) the bimolecular rate constant f ...	1979	262439
lipopolysaccharides from rickettsiaceae: limulus endotoxin assay and pathogenetic mediators in rickettsiosis.		1980	6107027
adenine nucleotide degradation by the obligate intracellular bacterium rickettsia typhi.	adenosine 5'-triphosphate (atp) was catabolized by whole cells and cell-free extracts of rickettsia typhi to adenosine 5'-diphosphate (adp) and then to adenosine 5'-monophosphate (amp), the end product of atp catabolism under the experimental conditions used. the only intermediate of the pathway from atp to amp which was identified by thin-layer chromatography and quantitated by the (14)c content was adp, whereas products such as adenine, adenosine, hypoxanthine, inosine, and inosine 5'-monophos ...	1980	6247288
in vitro stimulation of human peripheral blood lymphocytes by soluble and membrane fractions of renografin-purified typhus group rickettsiae.	cell-free extracts of disrupted renografin-purified rickettsia typhi and r. prowazekii were evaluated as antigens in lymphocyte transformation assays for cell-mediated immunity to typhus group rickettsiae in 19 individuals with and 9 without histories of exposure to these organisms. exposure consisted of clinical disease, vaccination with epidemic typhus vaccine, or occupational exposure to these agents. both the soluble and membrane fractions of disrupted purified rickettsiae were used, and tra ...	1980	6769803
development and characterization of high-titered, group-specific fluorescent-antibody reagents for direct identification of rickettsiae in clinical specimens.	rabbits were inoculated with purified antigen preparations of coxiella burnetii and representative species of the spotted fever and typhus groups of rickettsiae. their antibody responses were monitored by complement fixation tests; high-titered antisera were fractionated with ammonium sulfate and then labeled with fluorescein isothiocyanate by the dialysis method. the conjugates had homologous 3+ staining titers of 1:256 to 1:2,048 and did not exhibit nonspecific staining. the rickettsia rickett ...	1980	6769955
mechanisms of immunity in typhus infection: analysis of immunity to rickettsia mooseri infection of guinea pigs.	to study the mechanisms of immunity to rickettsia mooseri (r. typhi) infection, sera and splenic cells collected from nonimmune and immune guinea pigs were inoculated separately into syngeneic nonimmune recipients which were subsequently challenged intradermally. protection was measured by comparing the course of the challenge infections of recipients with infections initiated with the same rickettsial inocula in nonimmune animals. recipients of splenic cells collected 21 days after donor infect ...	1980	7380552
use of a sensitive microplate enzyme-linked immunosorbent assay in a retrospective serological analysis of a laboratory population at risk to infection with typhus group rickettsiae.	a microplate enzyme-linked immunosorbent assay (elisa), developed for the detection of antibodies to typhus group rickettsiae, was used to analyze human sera from individuals engaged directly or indirectly in rickettsial research. the earliest serum available from each of 112 individuals was tested for immunoglobulin m (igm) and igg antibodies against rickettsia typhi and rickettsia prowazekii by elisa at a 1:500 dilution. in at least one assay, nine sera had elisa optical densities of greater t ...	1980	6783677
[correlations between the processes of cell differentiation and development of blood vessels in the (chick embryo) adenohypohhysis].	utilizing semi-thin and, respectively, thick sections after indian ink injections, we have analyzed morphohistogenesis and vessel development in the adenohypophysis of chick embryos from the 4th to the 19th incubation day. our preliminary results indicate that a close correlation exists between cellular differentiation and vasculogenesis. vessels begin to enter the gland only at the 6th day when the first endocellular granules become detectable, and, from the 10th day, they markedly increase in ...	1980	550895
[mediterranean lymphoma in peru. its treatment with oral oxytetracycline].	a new peruvian case of malabsorption associated with primary diffuse lymphoma of the small bowel and mesenteric lymph glands is reported, having found no alpha chains either in blood or in urine. like in most of the 8 previous cases, medium oral doses of oxytetracycline supressed diarrhea, markedly atenuated steatorrhea, restored the sense of well-being to the patient, and significantly increased his body weight. critic is made on the denominations "mediterranean lymphoma" and "primary upper sma ...	1980	550791
[impressions with alginate].		1981	351780
antibodies to typhus in eastern nepal.		1981	6798725
platelet aggregation and stimulation of leucocyte procoagulant activity by rickettsial lipopolysaccharides in rabbits and in man.	the effects in vitro of 4 purified lipopolysaccharide (lps) preparations from rickettsiae on platelets and leucocytes were studied in rabbits and in man. all lps induced aggregation in rabbit platelet-rich plasma but to differing degrees. this activity was abolished by inactivation of complement. none of the preparations induced aggregation of human platelets. both rabbit and human leucocytes, when incubated with each of the rickettsial lps preparations, generated a potent procoagulant activity ...	1981	6781928
partial purification and characterization of the major species-specific protein antigens of rickettsia typhi and rickettsia prowazekii identified by rocket immunoelectrophoresis.	species-specific antigens from rickettsia typhi and rickettsia prowazekii were readily solubilized by french pressure cell extraction or sonication of renografin density gradient-purified rickettsiae and were identified by rocket immunoelectrophoresis. as measured by quantitative rocket immunoelectrophoresis, the species-specific typhus rocket antigens (stras) appeared to be proteins; they were denatured by heating at 56 degrees c for 30 min but not by 50 degrees c treatment, and they were sensi ...	1981	6783537
epidemic typhus (rickettsia prowazekii) in massachusetts: evidence of infection.		1981	6783902
isolation of species-specific protein antigens of rickettsia typhi and rickettsia prowazekii for immunodiagnosis and immunoprophylaxis.	a simple procedure for the selective isolation of the protective species-specific protein antigens (spas) of rickettsia typhi and rickettsia prowazekii was developed to permit use of the spas in the immunodiagnosis and immunoprophylaxis of typhus infections. although the spas were readily extracted from lysozyme- or detergent-treated rickettsiae, as measured by rocket immunoelectrophoresis, other polypeptides were also present, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresi ...	1981	6793620
detection of typhus antibodies by latex agglutination.	a latex test for assay of antibodies to endemic and epidemic typhus rickettsiae is simple, group-specific, sensitive, and reproducible. cross-reactivity within the typhus group was extensive.	1981	6780601
[analysis and improvement of culture processes of pathogenic microorganisms].		1981	6274109
murine and epidemic typhus rickettsiae: how close is their relationship?	typhus fever has occurred globally as epidemic and endemic disorders. in 1910, brill reported a typhus-like illness which zinsser and others determined to be recurrent epidemic typhus fever. maxcy, in 1926, proposed rodents and fleas as reservoir and vector, respectively, of endemic typhus, which dyer confirmed in 1930. animals experimentally infected with epidemic typhus (rickettsia prowazeki) are immune to murine typhus (rickettsia typhi) and vice versa. similar solid cross-immunity exists for ...	1982	6817526
ornithine metabolism in the genus rochalimaea.	ornithine metabolism was studied in two strains of the trench fever rickettsia rochalimaea quintana, fuller and guadalupe, and in the vole agent, a strain of rochalimaea but not necessarily of rochalimaea quintana. the metabolic activity of intact cells and cell-free extracts was measured by monitoring the evolution of 14co2 from [1-14c]ornithine. low levels of activity were obtained with all three strains, but requirements for the demonstration of this activity differed. with the cells of the f ...	1982	7061394
[clinico-histological picture report on several selected head tumors].		1982	220106
detection and characterization of mouse monoclonal antibodies to epidemic typhus rickettsiae.	a solid-phase immunofluorometric assay was used to detect mouse monoclonal antibodies to epidemic typhus rickettsiae, rickettsia prowazekii (the immunizing antigen), and to murine typhus rickettsiae, rickettsia typhi, a related antigen. of the 649 hybridoma cultures obtained, 628 contained antibodies either to r. prowazekii or to both r. prowazekii and r. typhi. a total of 72 cultures were cloned by limiting dilution and yielded 137 antibody-producing clones. of these, 104 produced antibodies sp ...	1983	6415092
rickettsial antibody prevalence in southern israel: igg antibodies to coxiella burnetii, rickettsia typhi, and spotted fever group rickettsiae among urban- and rural-dwelling and bedouin women.	a retrospective serological survey was carried out using sera obtained from women at childbirth in the southern desert region of israel to determine exposure experience to three rickettsial agents: coxiella burnetii, rickettsia typhi, and spotted fever group rickettsiae. using the indirect fluorescent antibody method for determining igg antibodies, it was found that about 40% of all sera examined demonstrated antibodies to one or more rickettsiae. bedouin women appeared to be at greater risk of ...	1983	6418016
rickettsia mooseri infection in the fleas leptopsylla segnis and xenopsylla cheopis.	detailed observations on the acquisition and propagation of experimental rickettsia mooseri infection in two species of fleas are presented. rickettsia mooseri infection became detectable by means of the direct fluorescent antibody test about 2 days earlier in leptopsylla segnis than in the putative vector, xenopsylla cheopis. by the 6th day after the infective feeding, the entire lining and the lumen of the midgut in l. segnis contained masses of rickettsiae and the agent was being passed in th ...	1983	6418017
role of t-lymphocytes in production of antibody to antigens of rickettsia tsutsugamushi and other rickettsia species.	the requirement of thymus-dependent lymphocytes for antibody production to rickettsia tsutsugamushi, rickettsia akari, rickettsia conorii, and rickettsia typhi was investigated by comparing antibody production in athymic (nu/nu) or thymus-bearing balb/c mice. athymic balb/c mice produced antibody after infection with r. akari, r. conorii, and r. typhi as measured by indirect fluorescent antibody titration or radioimmunoassay. antibody production in these mice was a great or greater than in the t ...	1983	6223883
experimental infection with rickettsia mooseri and antibody response of adult and newborn laboratory rats.	quantitative studies of selected features of peripherally induced rickettsia mooseri (= r. typhi) infection in rattus norvegicus-derived white laboratory rats revealed a unique association between microbe and amplifying vertebrate host which appears to be especially conducive to maintenance of the enzootic cycle. both adult and newborn (1-3 days old) rats were highly susceptible to percutaneous infection (id50 = approximately 1 organism), but neither showed signs of disease or died even when ino ...	1984	6486291
characteristics of rickettsia mooseri infection of normal and immune mice.	rickettsia mooseri infection initiated by subcutaneous injection has been studied in balb/c mice with the objective of developing a model for the study of immune mechanisms. characterization of infection included the following: measurement of the replication, dissemination, and clearance of rickettsiae; measurement of correlates of the immune response, including humoral antibody, hypersensitivity to subcutaneously inoculated rickettsial antigen, and activation of nonspecific macrophage microbici ...	1984	6546307
[rickettsiosis and chlamydiosis in hoggar (republic of algeria): epidemiological sampling].	the study was made on reservoirs of infections (rodent, sheep, goat, dromedary), vectors (louse) and human-receptors. examinations were made on smears examined with staining and indirect-fluorescente techniques and on sera examined by complement fixation test. chlamydia psittaci appears very often in rodent, sheep, goat, dromedary and man. antibodies against coxiella burneti were found present in sheep, goat but mainly in dromedary and less than 16 years old boys. two lice were carrier of ricket ...	1984	6548418
sensitization of mice to rickettsial toxin by coxiella burnetii.	intraperitoneal (ip.) inoculation with live or killed coxiella burnetii (c.b.) rendered mice more sensitive to intravenous (iv.) administration of a toxic live suspension of rickettsia typhi. sensitization of mice by live and killed c.b. was time-and dose-dependent. killed phase i and phase ii c.b. cells possessed a similar degree of sensitization, which was increased slightly by their preincubation with corresponding immune sera. lipopolysaccharide (lps)-protein complex extracted from phase i c ...	1984	6148856
characteristics of lymphoid cells that adoptively transfer immunity to rickettsia mooseri infection in mice.	the capacity of adoptively transferred immune lymphoid cells or passively transferred immune serum to alter the course of an established rickettsia mooseri (r. typhi) infection in the spleen was evaluated in balb/c mice. immune cells, but not immune serum, controlled the established infection. an effective lymphocyte was a t-cell which had to possess a capacity to divide.	1984	6608497
spotted fever and murine typhus in the negev desert region of israel, 1981.	during 1981, 52 cases of rickettsiosis caused by the spotted fever group of rickettsiae were reported in a population of 250 000 living in the negev desert region of israel. retrospectively, at least 5 of these cases were serologically shown to have been caused by rickettsia typhi, the agent of murine typhus. most cases of rickettsial disease occurred in the summer months and one half of all cases occurred in children aged 9 years or less. while the specificity of a physician's diagnosis of the ...	1984	6610498
[pleuropericarditis caused by rickettsia mooseri. an unusual manifestation of murine typhus].		1984	6739907
the preparation and use of a formulary in a provincial hospital.		1984	263384
the betrayal of youth.		1985	271692
[interaction of local anesthetics with secretory cells at the membrane level].		1985	2408715
rickettsiae and hosts.	protective immunity to rickettsia tsutsugamushi in a murine model is dependent upon the development of cell mediated immunity, as demonstrated by lymphocyte transfer, production of lymphokines and -interferon by thymus-derived lymphocytes, activation of macrophages by lymphokines and demonstration of delayed-type hypersensitivity response following exposure to these organisms. infection of mice with small numbers of rickettsia typhi inoculated by a peripheral route leads to a more complex patter ...	1985	2408453
mechanisms of immunity to infection with typhus rickettsiae: infected fibroblasts bear rickettsial antigens on their surfaces.	as with any immune response to infectious organisms, both antibody and t cell-mediated immune responses to infection with rickettsia typhi require the appropriate presentation of rickettsial antigens to immunocompetent cells. considering the obligate intracellular nature of rickettsiae, the exact mechanisms by which lymphocytes and macrophages encounter and respond to rickettsial antigens may depend on certain aspects of pathogenesis and on the availability of organisms or their antigens to cell ...	1985	2415459
[antigenic structure of the rickettsiae].		1985	2418607
in vitro antigen-specific antibody response to the species-specific surface protein antigens of typhus group rickettsiae by human peripheral blood mononuclear cells: generation of an antigen-dependent suppressor t cell.	we defined conditions suitable for in vitro synthesis of rickettsia-specific antibody by human pbmc cultured with the spa of rickettsia typhi or r. prowazekii and without addition of mitogens or polyclonal stimulators. antibody synthesis, as measured by an enzyme-linked immunosorbent assay, was cycloheximide-inhibitable and antigen-specific. pbmc from individuals with prior rickettsial infection made antibody, whereas pbmc from those receiving vaccine or with undetectable levels of serum anti-sp ...	1985	2579151
convulsive syndrome induced by the intracerebroventricular injection of alpha-difluoromethylornithine in rats.	in adult rats, the intracerebroventricular injection of alpha-difluoromethylornithine (dfmo), a polyamine antimetabolite which specifically inhibits ornithine-decarboxylase, induces a typical convulsive syndrome (ed50 = 100 micrograms/rat) and death (ld50 = 300 micrograms/rat). histological lesions are seen only with the highest doses and are essentially restricted to the ammon's horn neurones. since dfmo is currently undergoing clinical trials for use in cancer chemotherapy, including brain tum ...	1985	3925717
[production of chemotactic factor for mononuclear cells from igg cleavage with neutral thiol proteinase of dog pmn leukocyte].		1985	3897413
comparison of a latex agglutination procedure with the microimmunofluorescence test for rickettsia typhi.	sera submitted to the texas department of health for the serodiagnosis of rickettsia typhi were tested by the microimmunofluorescent antibody technique and a new latex agglutination procedure. results indicated that the latex agglutination test was sensitive and specific and would serve well as a first-line screening test for murine typhus.	1985	3920243
transmission of murine typhus rickettsiae by xenopsylla cheopis, with notes on experimental infection and effects of temperature.	in studies on experimental infection of rickettsia mooseri (= r. typhi) in xenopsylla cheopis and laboratory rats, it was found that 10 days after the infectious feeding, the fleas were voiding feces that were infective to rats upon inoculation. the feces remained infective for at least the duration of the experiment, and a quantity as small as 0.2 micrograms of feces would result in seroconversion of 67% of the rats upon inoculation. fleas were capable of transmitting the infection to rats as e ...	1985	3923852
evidence of spotted fever rickettsial infection in japan as demonstrated by the indirect immunoperoxidase test.		1985	3938518
lipid peroxidation-induced inhibition of gamma-aminobutyric acid uptake in rat brain synaptosomes: protection by glucocorticoids.	incubation of rat brain synaptosomes with xanthine and xanthine oxidase (x/xo) resulted in an inhibition of gamma-aminobutyric acid (gaba) uptake. the inhibitory effects of x/xo were temperature- and time-dependent, and were characterized by an increased km for gaba and a decreased vmax. inhibition of gaba uptake by x/xo was associated with both the formation of malonyldialdehyde (mda) and conjugated dienes, indicating that lipid peroxidation was involved. studies with catalase, superoxide dismu ...	1985	3882888
in vivo milk digestion in the calf abomasum. iii. amino acid compositions of the digesta leaving the abomasum.	calves were fed five different test meals: whole milk, skim milk, 3% whole casein solution, 3% whole casein in simulated milk ultrafiltrate, and whey. the digesta leaving the abomasum before feeding and during the first 7 postprandial hours were collected by fractions. after precipitation with 12% tca, the amino acid compositions of the sediments and the supernatants were determined and compared by multivariate analysis. the composition of prefeeding digesta was similar to that of gastric juice. ...	1985	4023394
[effects of thymosin on the humoral immune response to antibody-dependent cell cytotoxicity].		1985	4065002
transovarial transmission of murine typhus rickettsiae in xenopsylla cheopis fleas.	it has been generally accepted that infected fleas do not pass on rickettsia mooseri, or indeed any other known pathogen, to their progeny. it is reported here that such transovarial transmission does occur in laboratory-infected xenopsylla cheopis fleas. by means of the direct fluorescent antibody test, rickettsia mooseri was observed in cells of the hemolymph of infected fleas. as many as 11 percent of the adults and 2.9 percent of the larvae of the generation reared therefrom, had demonstrabl ...	1985	3966162
a possible role for natural killer cells in providing protection against plasmodium berghei in early stages of infection.	beige mutant mice, which are deficient in natural killer (nk) cells, exhibited a significantly higher parasitaemia than the parental c57bl/6 strain between days 4 and 10 after infection with plasmodium berghei. within 8-12 days after infection 70% of beige mice were dead but no deaths occurred in the parental strain until day 16. the median survival time of the beige mice (10 days) was significantly lower than that of the parental strain (22 days). it appears that nk cells may be protective in t ...	1985	3891603
cellular receptors for type beta transforming growth factor. ligand binding and affinity labeling in human and rodent cell lines.	type beta transforming growth factor (beta tgf) purified from human platelets to homogeneity as judged by nh2-terminal amino acid sequence analysis has been labeled with 125i to characterize its interaction with cellular receptors. binding of 125i-beta tgf to target cells is temperature- and time-dependent, specific, saturable, and reversible. about 1.6-1.9 x 10(4) binding sites/cell with high affinity for beta tgf (kd = 5.6-7.8 x 10(-11) m and 9.1-14 x 10(-11) m, respectively) are found in nrk- ...	1985	2982829
constitutive and conditional suppression of exogenous and endogenous genes by anti-sense rna.	plasmid dna directing transcription of the noncoding (anti-sense) dna strand can specifically inhibit the expression of several test genes as well as normal, endogenous genes. the anti-sense plasmid constructions can be introduced into eukaryotic cells by transfection or microinjection and function in both transient and stable transformation assays. anti-sense transcripts complementary to as little as 52 bases of 5' untranslated target gene mrna specifically suppress gene activity as well as, or ...	1985	2990048
human t helper cells specific for antigens of typhus group rickettsiae enhance natural killer cell activity in vitro.	the peripheral blood mononuclear cells (pbmc) from 5 individuals immune to typhus group rickettsiae and from 13 nonimmune individuals were stimulated in vitro for 7 days with typhus group rickettsial antigen (tgra). at the end of day 7, lysis of the natural killer (nk)-susceptible target k562 by these pbmc was determined. as controls, pbmc from both groups of donors were cultured in vitro for 7 days without antigen or were freshly isolated, and lysis of the k562 target was determined. there was ...	1986	2945787
[herpes genitalis, in prostitutes. detection of asymptomatic viral excretors and their epidemiological role].		1986	3027823
effects of "partial axotomy" upon synaptic function.	a lesion limited to the dorsal columns, at the level of l3-l4, was carried out in chronic cats. this operation produced a "partial axotomy" type of lesion on the ascending branches of ia hind limb afferents. two to six months after this operation, intracellular studies on l7-s1 motoneurons were carried out. similar studies were done in normal animals. the peak amplitude and the rate of rise (dv/dt) of heteronymous epsp's were studied during control conditions (sampling at 1 hz) and during the po ...	1986	3026830
an endemic focus of murine typhus in europe.		1986	3090154
serological cross-reactions between rickettsia typhi, proteus vulgaris ox19, and legionella bozemanii in a series of febrile patients.	the sera of 19 patients with a febrile disease of undetermined etiology were positive in the indirect immunofluorescence assay (ifa) to legionella bozemanii serogroup 1 (lb) and rickettsia typhi (rt). to both antigens, high titers of igg-class and igm-class antibodies were demonstrated. several of the patients also had positive ifa and weil-felix reactions to proteus vulgaris ox19 (px 19). a sharp reduction of the serotiters to all three antigens was achieved by absorption of the sera with any o ...	1986	3098699
a serological survey of scrub, tick, and endemic typhus in sabah, east malaysia.	a seroepidemiological survey of 837 people and 383 febrile patients was performed in rural areas of sabah. we determined that the rickettsial diseases scrub typhus and endemic typhus were uncommon causes of febrile illness, as was tick typhus, except in forest dwelling peoples. the rate of occurrence of sfgr specific antibody was 16.5% among 412 forest dwellers, indicating that tick typhus may be a frequent cause of illness in this population.	1986	3107139
mechanisms of immunity to rickettsial infection: characterization of a cytotoxic effector cell.	rickettsiae, as other intracellular bacteria, are relatively sequestered from the effects of antibody and local antibody-independent responses. considering the obligate intracellular nature of rickettsia, the exact mechanisms by which lymphocytes and macrophages encounter rickettsial antigens and eliminate the infection depends upon the appropriate presentation of antigen to the immune system. we demonstrate here that cells taken from the spleens of rickettsia typhi- or r. tsutsugamushi-infected ...	1986	3080523
lymphokine-activated-killer-mediated lysis of cells infected with typhus group rickettsiae can be inhibited by okt3 monoclonal antibody.	we examined the ability of monoclonal antibodies directed against lymphocyte surface antigens to block the lysis of typhus group rickettsia-infected cells by lymphokine-activated killer effectors. lysis was significantly inhibited by okt3 monoclonal antibody but not by okt4 or okt8 monoclonal antibody.	1986	3087881
the effects of alpha adrenergic blockade on arachidonic acid metabolism and shock sequelae in endotoxemia.	previous studies have suggested that increased alpha adrenergic activity stimulated prostaglandin synthesis and may be involved in the modulation of eicosanoid metabolism. these observations prompted investigation of the effect of the alpha adrenergic receptor antagonist phenoxybenzamine and the cyclo-oxygenase inhibitor indomethacin on endotoxin-induced shock severity, and on plasma immunoreactive itxb2 and i6-keto-pgf1 alpha, the stable metabolites of txa2 and pgi2, respectively. pretreatment ...	1986	3096591
prevalence and distribution of spotted fever and typhus infections in sierra leone and ivory coast.	a serosurvey for evidence of rickettsial infections was conducted in the rural populations of several tropical rain forest areas in sierra leone and ivory coast. seropositivity rates were surprisingly high in both countries, with more than 7% of the individuals in some districts having antibodies to spotted fever-group rickettsiae. no significant difference was found in the overall prevalence of diagnostic antibody titers to spotted fever-group rickettsiae in sierra leone (5.3%) and ivory coast ...	1986	3025125
metabolism of 4,4'-methylenebis(2-chloroaniline) by canine liver and kidney slices.	4,4'-methylenebis(2-chloroaniline) (mboca) metabolism in canine liver and kidney slices was investigated using hplc to separate the metabolites. liver slices metabolized 5-10% of the 14c-mboca in 60 min and produced seven metabolites resolved by hplc. the major metabolite, representing approximately 80% of the metabolism, was 2-amino-5-[(4-amino-3-chlorophenyl)methyl]-3-chlorophenyl hydrogen sulfate, previously identified as the major urinary metabolite in dogs. an mboca-glucoside was identified ...	1986	2870890
metabolism of 4,4'-methylenebis(2-chloroaniline) by canine liver and kidney slices.	4,4'-methylenebis(2-chloroaniline) (mboca) metabolism in canine liver and kidney slices was investigated using hplc to separate the metabolites. liver slices metabolized 5-10% of the 14c-mboca in 60 min and produced seven metabolites resolved by hplc. the major metabolite, representing approximately 80% of the metabolism, was 2-amino-5-[(4-amino-3-chlorophenyl)methyl]-3-chlorophenyl hydrogen sulfate, previously identified as the major urinary metabolite in dogs. an mboca-glucoside was identified ...	1986	2870890
altered cell cycle phase distributions in cultured human carcinoma cells partially depleted of polyamines by treatment with difluoromethylornithine.	we have studied the effects of partial polyamine depletion, induced by treatment with alpha-difluoromethylornithine (dfmo) on cell cycle phase distributions in five cultured human carcinoma cell lines. we used flow cytometry of cells stained with chromomycin-a3 and computer analysis to measure phase distributions of treated and control cultures. all five lines respond to 1-5 mm dfmo treatment with a total absence of measurable putrescine, a loss of greater than 90% of spermidine, and a 30-40% de ...	1986	3079590
suppression of migration inhibition factor (mif) production by mitomycins in vitro.	the effects of a series of mitomycins in a modified version of the migration inhibition factor (mif) test were investigated. this modified assay, which uses monocytes of the mouse cell line wehi3 as target cells for mif, discriminates between effects on the migrating monocytes, the production of mif by lymphocytes and the activity of the produced mif. the 7-methoxy mitomycins were found to be more active than the 7-amino and 7-hydroxy derivatives in suppressing both the spontaneous migration of ...	1986	3533763
suppression of migration inhibition factor (mif) production by mitomycins in vitro.	the effects of a series of mitomycins in a modified version of the migration inhibition factor (mif) test were investigated. this modified assay, which uses monocytes of the mouse cell line wehi3 as target cells for mif, discriminates between effects on the migrating monocytes, the production of mif by lymphocytes and the activity of the produced mif. the 7-methoxy mitomycins were found to be more active than the 7-amino and 7-hydroxy derivatives in suppressing both the spontaneous migration of ...	1986	3533763
studies on the mechanics of the normal human middle ear.	the middle ear was studied in temporal bone preparations using a laser-doppler interferometer. for measurements at a sound level of 80 db spl this method proved to be very reliable, as was shown by good reproducibility of results in experiments over more than 6 hours. the vibrations of the tympanic membrane and stapes footplate were studied from 200 hz to 10 khz and the results demonstrate a piston-like movement of the stapes footplate up to 120 db spl. the damping effect of the normal ear is lo ...	1986	3536194
characterization of human cytotoxic lymphocytes directed against cells infected with typhus group rickettsiae: evidence for lymphokine activation of effectors.	an in vitro culture and assay system was used to determine whether cytotoxic lymphocytes are generated in humans after rickettsial infection. peripheral blood mononuclear cells (pbmc) were obtained from six individuals with serologic evidence of prior infection with typhus group rickettsiae and from six nonimmune individuals. after pbmc from immune individuals were stimulated in vitro for 7 days with rickettsial antigen, they were capable of lysing typhus group rickettsia-infected, autologous ph ...	1986	3081648
structures of neorustmicins b, c and d new congeners of rustmicin and neorustmicin a.		1986	3759645
splenectomy: an old issue of current controversy.		1986	3957756
morphological effects of gas compression on the cortical vitreous.	we used scanning electron microscopy to study vitreous in normal and perfluorocarbon gas-treated eyes of cynomolgus monkeys. the cortical vitreous of the normal (control) eye appeared to be a lamellar structure composed of sheets of collagen mesh. in the gas-treated eye there was shrinking and tearing of the lamellae. at six and 12 weeks after gas injection there was membrane formation at the edges of the torn lamellae.	1986	3741253
study of a hydrophobic site on bovine alpha-lactalbumin by labeling with [125i]-tid.	the hydrophobic, photoreactive probe 3-(trifluoromethyl)-3-(m-[125i]iodophenyl) diazirine ([125i]tid) labels apo-bovine alpha-lactalbumin but much less his ca2+-form. the labeling of the apo-form is strong at protein concentrations of 0.5 mg ml-1 and increases with increasing concentration. furthermore, increasing concentrations of nacl, decrease the labeling of apo-alpha-lactalbumin with [125i]tid.	1986	3741424
lysosomal membrane stabilization by enfenamic acid.		1986	3583334
prevalence of antibodies to legionella species in a series of patients in israel.	a collection of serum specimens from 77 patients at various hospitals or clinics in israel was used to determine the usefulness of the enzyme-linked immunosorbent assay (elisa) with a multivalent antigen for the detection of legionella antibodies. rickettsial infection rather than legionellosis was suspected in most of these patients. the multivalent antigen was derived from legionella pneumophila serogroups 1-6, l. bozemanii wiga, and l. micdadei tatlock. a preliminary test of the multivalent a ...	1986	2419278
increase of enkephalin and decrease of substance p immunoreactivity in the dorsal and ventral striatum of the rat after midbrain 6-hydroxydopamine lesions.	unilateral 6-hydroxydopamine lesions of the mesostriatal dopaminergic system in the rat resulted in a decrease of substance p-immunoreactivity in the ventral striatum, and in a heterogeneously distributed increase of enkephalin-immunoreactivity in the dorsal and ventral striatum and the globus pallidus. the respective decrease and increase are caused by a lower or higher staining intensity of the peptidergic fibers, whereas no changes were found in the cell bodies.	1987	2440523
[effect of duration of sexual abstinence on the quality of human sperm in andrologic patients].	between 1981 and 1984, 1063 patients (average age 32 years) were examined because of childlessness in marriage. the following parameters were evaluated in a first and second investigation: amount of ejaculate (ml), number of spermatozoa (mill./ml), motility (percentage of highly motile, motile, and non-motile spermatozoa immediately, 30, and 240 minutes after ejaculation), morphology (percentage of normally shaped spermatozoa), ph value, and fructose concentration (micrograms/ml), with special i ...	1987	3617855
effects of ouabain on cytosolic calcium in lymphocytes, platelets and adrenomedullary cells.	it has been suggested that a circulating inhibitor of na/k atpase can stimulate natriuresis and cause vasoconstriction in essential hypertension by stimulating transmembrane na/ca exchange to produce increased cytosolic concentrations of ionized calcium ([ca++]i) in renal tubular and arteriolar smooth muscle cells. if this inhibitor affected [ca++]i in all cell types, then clinical assays for its presence could be applied to easily accessible cells such as blood cells or platelets, and the inhib ...	1987	3429864
[free serotonin levels in injured skin in the early pre- and postmortem periods].		1987	3433317
[buerger's disease: etiologic role of the rickettsiae?].	specific seroagglutination tests were performed in 70 patients with buerger's disease to detect possible antibodies to rickettsia, the test being repeated in 8 cases after activation by doxycycline administration over 10 days. in 21 patients a weil-felix test was also carried out. tests were assessed as positive in 44 cases (62.8%): 18 times for rickettsia q. 18, 14 times for rickettsia mooseri, 13 times for rickettsia burnetii, 5 times for rickettsia conorii and 3 times for rickettsia prowazeki ...	1987	3559416