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bartonella infections. 19979189670
[cat-scratch disease--an overlooked disease in denmark?].only one patient with cat-scratch disease (csd) has been reported in denmark. a case and retrospective investigation among patients admitted to the ward is presented. over a period of 3.5 years, six patients were found to have suffered from csd. the yearly incidence was calculated to 2.6/100,000. the patients were tested for antibodies against bartonella (rochalimaea) henselae with a new test developed at the danish serum institute. only two of the patients with csd had titres of antibodies high ...19979190717
bartonella clarridgeiae, a newly recognized zoonotic pathogen causing inoculation papules, fever, and lymphadenopathy (cat scratch disease).shortly after adopting a 6-week-old cat, a veterinarian was bitten on the left index finger. within 3 weeks, he developed headache, fever, and left axillary lymphadenopathy. initial blood cultures from the cat and veterinarian were sterile. repeat cultures from the cat grew bartonella-like organisms with lophotrichous flagella. sera from the veterinarian were not reactive against bartonella henselae, b. quintana, or b. elizabethae antigens but were seroreactive (reciprocal titer, 1,024) against ...19979196200
culture-negative endocarditis: contribution of bartonella infections.two cases of bartonella endocarditis are described: one in a 55 year old homeless alcoholic man, caused by bartonella quintana; the other in a 41 year old male with a history of exposure to cat fleas, caused by b henselae. serological testing and polymerase chain reaction of the excised valves were used to identify the organisms. false positive serology for chlamydia was detected in one case.19979196420
detection of antibodies to bartonella henselae in clinically diagnosed cat scratch disease.to determine the usefulness of an indirect immunoflourescence antibody test for antibodies to bartonella henselae in diagnosing cat scratch disease (csd).19979196486
trench fever among homeless people in marseille, france: a seroprevalence survey. 19979196653
never discount an animal vector. 19979197061
[cat-scratch disease: historical, clinical, phylogenetic and taxonomic aspects].the cat-scratch disease (csd) is known as a nosological entity since 1950. it was diagnosed by the clinical symptoms, epidemiologic data, and the intracutaneous test of hanger and rose. the aetiologic agent is bartonella (formerly rochalimaea) henselae occurring in thirty to fifty percent of healthy cats. the gramnegative alpha-2-proteobacteria cause the csd but also fever in healthy humans. patients suffering from aids show bacillary angiomatosis, bacillary peliosis hepatis, endocarditis, and s ...19979198973
[cat scratch disease and associated infections].cat scratch disease (csd) was first described in france by debré et al. in 1950, yet the causative bacterial agent of csd remained obscure until 1992, when bartonella (formerly rochalimaea) henselae was implicated in csd by serological and microbiologic studies. b. henselae had been linked initially to bacillary angiomatosis (ba), but also bacillary peliosis, relapsing bacteremia and endocarditis. cats are healthy carriers of b. henselae and b. clarridgeiae, and can be bacteremic for months to y ...19979203735
molecular diagnosis of deep nodular bacillary angiomatosis and monitoring of therapeutic success.a 51-year-old human immunodeficiency virus (hiv)-positive male patient (cdc stage 3c) had had a painful nodule on his external ankle joint for 10 months. a biopsy suggested bacillary angiomatosis, but kaposi's sarcoma could not be excluded. rods were detectable in lesional skin by a warthin-starry stain. a 298 base pair (bp) gene fragment specific for bartonella species was amplified from lesional skin and direct nucleotide sequence analysis of the amplification product clearly identified barton ...19979205511
experimental infection of young specific pathogen-free cats with bartonella henselae.eighteen 12-week-old specific pathogen-free cats, blood culture- and serum antibody-negative for bartonella henselae, were randomly allocated to groups and were intravenously inoculated with 10(10) (group 1), 10(8) (group 2), or 10(6) (group 3) b. henselae or with saline (group 4) or were not inoculated (group 5). cats were humanely killed at 2, 4, 8, 16, and 32 weeks after inoculation. all b. henselae-inoculated cats were bacteremic by 2 weeks after infection. bacteremia persisted until 32 week ...19979207369
application of polymerase chain reaction assay in the diagnosis of orbital granuloma complicating atypical oculoglandular cat scratch disease.parinaud oculoglandular syndrome is uncommon. most cases are caused by cat scratch disease (csd), recently discovered to be associated with the pathogen bartonella henselae. before isolation of the micro-organism, diagnosis relied on the presence of characteristic clinical features. however, atypical cases could cause diagnostic problems. with the development of an indirect fluorescent antibody test and polymerase chain reaction (pcr) assay, oculoglandular csd can be diagnosed readily.19979224472
rapid polymerase chain reaction-based detection of the causative agent of cat scratch disease (bartonella henselae) in formalin-fixed, paraffin-embedded samples.bartonella (formerly rochalimaea) henselae (bh) plays a central role in cat scratch disease. a polymerase chain reaction (pcr)-based assay that can detect bh dna in formalin-fixed, paraffin-embedded (ff-pe) samples would have utility in the evaluation of processed lymph nodes suggestive of this disorder. fresh or ff-pe cultures of bh and related species were analyzed. thirteen lymph nodes (12 ff-pe and one fresh cell suspension) with necrotizing suppurative granulomatous inflammation and seven f ...19979224751
the 75-kilodalton antigen of bartonella bacilliformis is a structural homolog of the cell division protein ftsz.a genomic library of bartonella bacilliformis was constructed and screened with human anti-bartonella serum from a patient with the chronic, verruga peruana phase of bartonellosis. an immunoreactive clone isolated from this library was found to code for a 591-amino-acid protein with a high degree of sequence similarity to the ftsz family of proteins. the degree of amino acid identity between the b. bacilliformis protein (ftsz[bb]) and the other ftsz proteins is especially pronounced over the n-t ...19979226264
molecular diagnosis of cat scratch disease: a two-step approach.amplification of bartonella henselae dna has been proposed as a diagnostic test for cat scratch disease (csd). the sensitivities of the following three pcr assays were compared. pcr/rrna with universal primers amplifies part of the 16s rrna gene, followed by hybridization with a specific b. henselae probe; pcr/cs and pcr/hsp amplify portions of the glta and the htra genes, respectively, each followed by restriction fragment length polymorphism analysis. the threshold of detection of b. henselae ...19979230357
pitfalls and fallacies of cat scratch disease serology: evaluation of bartonella henselae-based indirect fluorescence assay and enzyme-linked immunoassay.the diagnostic value of the detection of immunoglobulin g (igg) and igm by bartonella henselae-based indirect fluorescence assay (ifa) and enzyme-linked immunoassay (eia) for the diagnosis of cat scratch disease (csd) was evaluated. the ifa was performed either with b. henselae that was cocultivated for a few hours with vero cells or with noncocultivated b. henselae as the antigen. additionally, the performance of a bartonella pcr hybridization assay based on the 16s rrna gene was determined and ...19979230358
pcr amplification and comparison of nucleotide sequences from the groesl heat shock operon of ehrlichia species.degenerate pcr primers derived from conserved regions of the eubacterial groesl heat shock operon were used to amplify groesl sequences of ehrlichia equi, ehrlichia phagocytophila, the agent of human granulocytic ehrlichiosis (hge), ehrlichia canis, bartonella henselae, and rickettsia rickettsii. the groesl nucleotide sequences were less conserved than the previously determined 16s rrna gene sequences of these bacteria. a phylogenetic tree derived from deduced groel amino acid sequences was simi ...19979230387
coinfection with bartonella clarridgeiae and bartonella henselae and with different bartonella henselae strains in domestic cats.bartonella clarridgeiae and several strains of bartonella henselae, the agent of cat scratch disease, with variations in the 16s rrna gene have been found to infect the blood of cats. an epidemiologic study of bartonella infection in domestic french cats revealed that of 436 cats sampled, 5 cats (1.1%) were coinfected with b. henselae and b. clarridgeiae and 2 cats (0.5%) were coinfected with two strains of b. henselae with variations in the 16s rrna gene, b. henselae type i and type ii. in an i ...19979230394
bartonellosis and human immunodeficiency disease (aids): l-forms as persisters, activating factors, and mechanism of disease.bartonella, genus proteus, can cause immunodepressive disease. the organisms, in parasitized red blood cells, may invade the brain and every other system and space in the human body. bartonella henselae is proposed to have a role in the pathogenesis of acquired immunodeficiency syndrome (aids) encephalopathy. bartonella bacilliformis produces two known toxins that can induce spasm and angiomatosis, respectively, and manifest as diseases associated with symptomatic aids. the skin lesions of barto ...19979247895
bacillary angiomatosis, cat-scratch disease, and bartonellosis: what's the connection? 19979248882
sternal abscess due to bartonella (rochalimaea) henselae in a renal transplant patient.bartonella henselae, previously called rochalimaea henselae, is the causative agent of cat scratch disease (csd) in immunocompetent subjects and bacillary angiomatosis in immunocompromised ones. bone lesions are common in bacillary angiomatosis, but not in csd. we present the case of a patient with a renal transplant treated by immunosuppressive therapy who developed a sternal abscess with a histological pattern of csd. the ct pattern was that of a lytic bone lesion with adjacent fluid collectio ...19979259103
immunologic response to bartonella henselae as determined by enzyme immunoassay and western blot analysis.bartonella henselae is now regarded as the etiologic agent of cat-scratch disease and a cause of bacillary angiomatosis. we examined the human immune response to bartonella henselae infection using a newly developed enzyme immunoassay (eia) and a western blot procedure using outer-membrane proteins. the eia showed 98.6% and 91.4% agreement with an indirect fluorescence method (ifa) for detection of igm and igg antibodies, respectively. by using western blot analysis, reactivity to an 8-kd band s ...19979260762
ease of isolation and semiquantitative culture of bartonella henselae from cats in melbourne. 19979271032
current knowledge of bartonella species.bartonella species are now considered emerging pathogens. of the 11 currently recognized species, four have been implicated in human disease, although only two have been encountered in europe. bartonella quintana infections are now being diagnosed among the urban homeless and deprived, manifesting as trench fever, and bartonella henselae has been shown to be the causative agent of cat scratch disease. both species also cause a variety of hiv-associated infections, including bacillary anglomatosi ...19979272384
heat shock response and groel sequence of bartonella henselae and bartonella quintana.transmission of bartonella species from ectoparasites to the mammalian host involves adaptation to thermal and other forms of stress. in order to better understand this process, the heat shock response of bartonella henselae and bartonella quintana was studied. cellular proteins synthesized after shift to higher temperatures were intrinsically labelled with [25s]methionine and analysed by gel electrophoresis and fluorography. the apparent molecular masses of three of the major heat shock protein ...19979274034
prevalence of bartonella species in domestic cats in the netherlands.cats have been shown to provide the only known reservoir of bartonella henselae, the causative agent of cat scratch disease. to determine the prevalence of bartonella bacteremia and antibodies in dutch cats, blood samples from 113 cats from shelters (sheltered cats), 50 pet cats, and 25 specific-pathogen-free (spf) cats were analyzed. culture and subsequent pcr-restriction fragment length polymorphism (rflp) analysis of the 16s-23s rrna intergenic region and 16s rrna gene pcr-hybridization assay ...19979276397
serological cross-reactions between bartonella and chlamydia species: implications for diagnosis.diagnosis of chlamydia or bartonella infections continues to rely mainly on serology. however, serological cross-reactions between members of these genera have recently been described. sera from eight patients originally diagnosed as having chlamydia pneumoniae endocarditis reacted with both chlamydia sp. and bartonella quintana antigens (microimmunofluorescence technique). adsorption of sera with b. quintana or c. pneumoniae antigens removed anti-c. pneumoniae antibodies, whereas adsorption wit ...19979276403
central nervous system infection associated with bartonella quintana: a report of two cases. 19979282717
bartonella (rochalimaea) henselae in southern africa--evidence for infections in domestic cats and implications for veterinarians.substantial evidence has recently accumulated showing domestic cats to be the principal reservoirs of bartonella henselae, the aetiological agent of human diseases including cat-scratch disease, bacillary angiomatosis, bacillary peliosis and a febrile bacteraemia syndrome. to determine the prevalence of antibodies reactive with bartonella henselae in cats from southern africa, indirect fluorescent antibody assays were carried out on feline sera from south africa and zimbabwe. overall, 23% (39/17 ...19969284029
flea-borne rickettsioses: ecologic considerations.ecologic and economic factors, as well as changes in human behavior, have resulted in the emergence of new and the reemergence of existing but forgotten infectious diseases during the past 20 years. flea-borne disease organisms (e.g., yersinia pestis, rickettsia typhi, r. felis, and bartonella henselae) are widely distributed throughout the world in endemic-disease foci, where components of the enzootic cycle are present. however, flea-borne diseases could reemerge in epidemic form because of ch ...19979284376
bartonellosis in ecuador: serosurvey and current status of cutaneous verrucous disease.human bartonellosis is a classically biphasic disease caused by infection with the alpha-2 proteobacteria bartonella bacilliformis, which is phylogenetically related to the etiologic agents of cat scratch disease, bacillary angiomatosis, and trench fever. in ecuador, typical bartonellosis has remained endemic for the past century in highland provinces near the peruvian border. during the past six years, public health officials have noted an increasing number of atypical cases in which monophasic ...19979288812
parinaud syndrome caused by bartonella henselae: case report.the authors report a case of parinaud syndrome (conjunctivitis with pre-auricular satellite adenitis) caused by bartonella henselae, the etiologic agent of cat scratch disease. the etiologic assessment of this case was performed by serum indirect immunofluorescence reaction and allowed for a better therapeutics and follow up, avoiding ineffective antibiotics and surgical interventions.19969293091
[bartonella henselae infection in immunocompetent patients: cat scratch disease].cat scratch disease, whose etiologic agent is bartonella henselae, is a benign disease in immunocompetent subjects, characterized by lymphadenopathy of prolonged course and occasional involvement of other organs such as liver, spleen, central nervous system, eye and lung. in immunocompromised patients, the infection is bacteremic and disseminated.19969293099
bartonella quintana invades and multiplies within endothelial cells in vitro and in vivo and forms intracellular blebs.bartonella quintana, the aetiologic agent of trench fever, has recently been implicated in culture-negative endocarditis and bacteraemia amongst homeless people. b. quintana is a fastidious slow-growing organism. a tissue culture system of human endothelial cells was developed in which b. quintana grew intracellularly. observation of the different steps during infection of these cells demonstrated that the bacteria adhered to and penetrated the cells by phagocytosis. during the preadherence stag ...19969296106
bacillary angiomatosis and other bartonella species infections.infections with organisms of the genus bartonella, for many years important only in south and central america, have assumed significance in developing countries, especially in conjunction with the advent of the pandemic of the human immunodeficiency virus infection. new molecular and culture techniques have determined that these organisms cause new diseases such as bacillary angiomatosis as well as diseases the etiology of which have been unknown such as cat scratch disease. in this article, the ...19979300630
cloning, functional expression, and complementation analysis of an inorganic pyrophosphatase from bartonella bacilliformis.we have cloned the inorganic pyrophosphatase gene (ppa) from the facultative intracellular pathogen bartonella bacilliformis and characterized its encoded product. the 531-bp gene is located approximately 1 kb downstream of, and in opposite orientation to, the invasion-associated locus (ialab) of b. bacilliformis. the predicted protein encoded by ppa is 177 amino acid residues, which is in agreement with in vitro and in vivo synthesis of a protein with an apparent molecular mass of 22-23 kda. th ...19979304784
cat-scratch encephalopathy.we present a case of cat-scratch disease in a 9-year-old girl, complicated by encephalopathy and seizures. bartonella (formerly rochalimaea) henselae is the causative agent in cat-scratch disease; methods now available for detection of this pleomorphic, gram-negative bacterium, including polymerase chain reaction amplification and indirect fluorescence antibody testing, may lead to changes in standard criteria used to verify a diagnosis of cat-scratch disease.19979305359
human zoonotic infections transmitted by dogs and cats.dogs and cats are the 2 most common household pets. however, they may be a direct or indirect source of human infections. this article aims to familiarize physicians with some common and uncommon bacterial, rickettsial, parasitic, and fungal zoonotic infections of dogs and cats. animal bites with or without infection continue to be a common problem. treatment of infected animal bites must include early debridement and concern for organisms from the mouth flora of the animal. the diagnosis and tr ...19979308505
[atypical cat-scratch disease: a case report of splenic granulomatosis].generally cat-scratch disease is a benign inflammatory adenopathy. the authors describe an atypical form of this disease, characterized by persistent fever and splenic granulomatosis requiring a diagnostic and therapeutic prolonged effort. they point out the important role of new immuno-fluorescent techniques to exactly identify the bacterium--bartonella henselae--causing cat-scratch disease and suggest to include cat-scratch disease among the causes of unknown origin fever.19979312753
[late lymphoadenopathy in cat-scratch disease]. 19979324701
seroprevalence of bartonella henselae infection and correlation with disease status in cats in switzerland.the prevalence of infection with bartonella henselae was investigated in cats from different areas of switzerland. serum samples of 728 cats were examined for antibodies to b. henselae by immunofluorescent antibody testing, and the results were analyzed with a view to a possible correlation between a positive titer and signalment, clinical signs, infection with feline leukemia virus (felv), feline immunodeficiency virus (fiv), feline coronavirus (fcov), or feline spumavirus (fesfv), and the livi ...19979350752
evaluation of commercial slides for detection of immunoglobulin g against bartonella henselae by indirect immunofluorescence.four commercial slides were compared with in-house slides for the detection of immunoglobulin g (igg) against bartonella henselae in 58 healthy persons from a rural region by an indirect immunofluorescence assay. mrl-ba slides (mrl diagnostics, usa) and virion slides (virion, switzerland) with agar-derived bartonella henselae showed igg titers of > or = 1:256 in 44.8% and 51.7%, respectively, whereas bion slides (bios, germany), mrl-vero slides (mrl diagnostics), and in-house slides with cell-as ...19979352257
bartonella henselae-associated acute multifocal retinitis in a patient with acquired immunodeficiency syndrome. 19979355199
analysis of 36-kilodalton protein (papa) associated with the bacteriophage particle of bartonella henselae.a library of bartonella henselae dna was screened with antibody raised to the bacteriophage particle associated with this organism. a clone was isolated that expresses a 36-kd protein (termed papa for particle-associated protein) when examined by immunoblot analysis using antibody raised to the particle. southern blot hybridization indicates that the gene is present on the bacterial chromosome and packaged into the 14-kb particle-associated dna. a papa-specific probe hybridized to multiple bands ...19979364933
american academy of dermatology 1997 awards for young investigators in dermatology. pathogenesis of bartonella henselae in cutaneous and systemic disease. 19979366828
efficacy of enrofloxacin or doxycycline for treatment of bartonella henselae or bartonella clarridgeiae infection in cats.enrofloxacin and doxycycline are antimicrobial agents used to treat bacterial diseases of cats. in vitro susceptibility data indicate that either drug should be effective against bartonella species. in vivo efficacies of these drugs for eradication of chronic bartonella henselae or bartonella clarridgeiae infections were examined in 18 experimentally infected cats and 25 naturally exposed cats treated with enrofloxacin (22.7 mg given orally [po] every 12 h [q12h] [14 days, n = 10; 28 days, n = 1 ...19979371348
interaction of bartonella henselae with endothelial cells results in bacterial aggregation on the cell surface and the subsequent engulfment and internalisation of the bacterial aggregate by a unique structure, the invasome.vascular colonisation by bartonella henselae may cause vaso-proliferative tumour growth with clumps of bacteria found in close association with proliferating endothelial cells. by using b. henselae-infected human umbilical vein endothelial cells as an in vitro model for endothelial colonisation, we report here on a novel mechanism of cellular invasion by bacteria. first, the leading lamella of endothelial cells establishes cellular contact to sedimented bacteria and mediates bacterial aggregatio ...19979378764
[cat scratch disease. diagnostic and therapeutic considerations]. 19979382360
distribution, diversity, and host specificity of bartonella in rodents from the southeastern united states.a number of bartonella isolates were obtained from seven species of rodents sampled from 12 geographic sites representing the major biotic communities of the southeastern united states. bartonella were isolated from the blood of 42.2% of 279 tested rodents. the highest prevalence of infection typically occurred among the most commonly captured species in the rodent community. four phylogenetic groups, uniting 14 genotypic variants of bartonella, were identified by sequence analysis of the citrat ...19979392599
bartonella-like erythrocyte inclusions in thrombotic thrombocytopenic purpura. 19979393349
possible role of lutzomyia maranonensis and lutzomyia robusta (diptera: psychodidae) as vectors of human bartonellosis in three provinces of region nor oriental del marañon, peru. 19979394538
[a case of cat scratch disease identified by an elevated bartonella henselae antibody level using enzyme immunoassay].a 68-year-old male was admitted to our hospital because of fever and a 2-week history of inguinal adenomegaly. since he owned a cat, cat scratch disease was suspected. but it was necessary to distinguish cat scratch disease from lymphoma type adult t-cell leukemia because he showed a high level of antibody against htlv-1. an excisional biopsy of the inguinal node was performed. histopathologic examination revealed abscess-forming granulomatous lymphadenitis compatible with cat scratch disease. a ...19979394562
inflammatory mass of the optic nerve head associated with systemic bartonella henselae infection. 19979400801
molecular epidemiology of bartonella infections in patients with bacillary angiomatosis-peliosis.bacillary angiomatosis and bacillary peliosis are vascular proliferative manifestations of infection with species of the genus bartonella that occur predominantly in patients infected with the human immunodeficiency virus. two species, b. henselae and b. quintana, have been associated with bacillary angiomatosis, but culture and speciation are difficult, and there has been little systematic evaluation of the species-specific disease characteristics. we studied 49 patients seen over eight years w ...19979407154
of cats, humans, and bartonella. 19979407161
comparison of the abilities of proteins from bartonella bacilliformis and bartonella henselae to deform red cell membranes and to bind to red cell ghost proteins.infections in humans by bartonella bacilliformis, but not bartonella henselae, are characterized by invasion of red cells. supernatants of culture medium from b. bacilliformis and b. henselae each contain a protein which causes invagination of membranes of human red cells and formation of intracellular vacuoles. these two proteins are very similar in molecular mass, heat stability and mechanism of action. b. henselae does not bind to human red cells, but human red cell ghost membrane proteins we ...19979418257
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 1-1998. an 11-year-old boy with a seizure. 19989424578
[rickettsioses: the epidemiological assessment].microbe of taxonomical families rickettsiaceae aceal and bartonellaceae of rickettsiales order have caused not less than 14 nosological forms of disease among people in different parts of the world. about 8 of them--in russia and in the former soviet republics. these diseases are not unequivocal from epidemiological point of view. trench, marseilles and other forms of fever, murine typhus, vesicules rickettsia, etc. have been liquidated and never recurred for 30-40 years. prowazek's [correction ...19979424811
diagnostic and therapeutic challenges. 19979428019
[bartonella infections. former infectious diseases return]. 19979432482
neuroretinitis and encephalopathy due to bartonella henselae infection. 19979448896
radiological case of the month. granulomatous hepatitis in cat-scratch disease. 19989452714
bartonella henselae as a cause of prolonged fever and fever of unknown origin in children.a prospective evaluation of 146 children with fever of unknown origin (fuo) and prolonged fever was performed from 1990 to 1996. fuo was defined as a documented daily temperature of > or = 38 degrees c for at least 14 days without diagnostic signs or symptoms. prolonged fever was defined as fever for at least 14 days and no diagnosis at the time of referral for evaluation. an established diagnosis was made for 84 (57.5%) of 146 patients. the most common infectious disease diagnoses were epstein- ...19989455513
in search of a bacterial species definition.the bacterial species concept was examined within the framework of plant and animal associated alpha-2 proteobacteria, taking into consideration the phylogenetic, taxonomic and biological approaches as well as the microbiologists' perception. the virtue of the phylogenetic approach is that it gives an evolutionary perspective of the bacterial lineage; however the methods used possess low resolution for defining species located at the terminal branches of the phylogenetic trees. the merit of the ...19979458988
isolation, sequencing and expression of the gene encoding a major protein from the backteriophage associated with bartonella henselae.the gene encoding a 31-kda major protein (pap31) associated with the bacteriophage harbored in bartonella henselae was cloned and sequenced. analysis of the resulting sequence revealed an open reading frame of 837 nucleotides coding for a protein of 279 amino acids. pap31 was then subcloned downstream of the lacz promoter in puc19. pap31 was amplified by polymerase chain reaction, and the linear amplicon was used as template for in-vitro transcription and translation. a protein with an apparent ...19989461414
immunopurified extracellular bartonella henselae antigen for detecting specific antibodies by enzyme immunoassay.protein antigens of bartonella henselae bacterial sonicate supernatant and concentrated cell-free culture filtrate were examined by sds-page. the sonicate supernatant gave 38 bands and the culture filtrate at least 21, of which 18 were of bacterial origin. immunoblotting against 13 monoclonal antibodies obtained from mice infected with live b. henselae showed that 10 of these antibodies reacted with a narrow 225 kda band and varying smears of bands ranging from 36 to 240 kda in the sonicate, but ...19979463512
an outbreak of bartonellosis in zamora chinchipe province in ecuador.we report an outbreak of human bartonellosis in zamora chinchipe province in ecuador, which occurred in 1995-1996. nineteen cases were seen, of which 18 presented with classical oroya fever (fever and profound anaemia) and one with verruga peruana; 11 of the cases (58%) had positive blood films containing bartonella bacilliformis. the houses of cases and neighbouring controls were visited; blood samples for thin films and cultures were collected from members of each house and a questionnaire was ...19979463663
cosegregation of a novel bartonella species with borrelia burgdorferi and babesia microti in peromyscus leucopus.during surveillance for various tickborne pathogens in the upper midwest during the summer and early fall of 1995, a bartonella-like agent was detected in the blood of mice that were concurrently infected with borrelia burgdorferi or babesia microti (or both). the organism was isolated in pure culture after inoculation of blood from wild-caught mice into c.b-17 scid/scid mice. phylogenetic analysis of the 16s rrna and the citrate synthase genes showed that the novel bartonella species and a bart ...19989466529
development and evaluation of a pcr-based assay for detection of haemobartonella felis in cats and differentiation of h. felis from related bacteria by restriction fragment length polymorphism analysis.the 16s rrna gene of haemobartonella felis was amplified by using universal eubacterial primers and was subsequently cloned and sequenced. based on this sequence data, we designed a set of h. felis-specific primers. these primers selectively amplified a 1,316-bp dna fragment of the 16s rrna gene of h. felis from each of four experimentally infected cats at peak parasitemia. no pcr product was amplified from purified dna of eperythrozoon suis, mycoplasma genitalium, and bartonella bacilliformis. ...19989466759
bartonella henselae antibody prevalence in free-ranging and captive wild felids from california.in order to determine the importance of wild felids in the epidemiology of bartonella spp. infection, 136 nobuto strips or serum samples from free-ranging mountain lions (felis concolor) and bobcats (felis rufus) captured in california (usa) between 1985 and 1996 were tested for b. henselae antibodies (titer > or = 1:64) using an immunofluorescence test. similarly, 124 serum samples from 114 captive wild cats representing 26 species or subspecies collected between 1991 and 1995 were retrieved fr ...19989476226
seroprevalence of bartonella henselae in cattery cats: association with cattery hygiene and flea infestation.we attempted to determine what factors were common to catteries with high bartonella henselae antibody prevalence compared with catteries with low prevalence, in order to contribute to better guidelines for the choice of a safe pet cat. the overall seroprevalence in 11 catteries from diverse geographical locations in north america in the present study was 35.8%. there was evidence of b. henselae exposure in all 11 homes tested, with 5 catteries being heavily infected. the distribution of b. hens ...19989477525
hypercalcemia due to endogenous overproduction of active vitamin d in identical twins with cat-scratch disease.the extrarenal synthesis of active vitamin d sterols has a central causative role in the hypercalcemia associated with various granulomatous diseases.19989480364
bartonella-like organisms. 19989492812
bartonella-associated infections.bartonella-associated infections occur in immunocompetent and immunocompromised patients. the spectrum of diseases caused by bartonella species has expanded and now includes cat-scratch disease, bacillary angiomatosis, bacillary peliosis, bacteremia, endocarditis, and trench fever. most bartonella-associated infections that occur in north america and europe are caused by b. henselae or b. quintana. the domestic cat serves as the major reservoir for b. henselae; the reservoir for the modern day b ...19989494835
clinical and microbiological features of refractory periodontitis subjects.the purpose of this investigation was to compare the clinical parameters and the site prevalence and levels of 40 subgingival species in successfully treated and refractory periodontitis subjects. 94 subjects received scaling and root planing and if needed, periodontal surgery and systemically administered tetracycline. 28 refractory subjects showed mean full mouth attachment loss and/or > 3 sites showing attachment loss > 2.5 mm within 1 year post-therapy. 66 successfully treated subjects showe ...19989495617
bartonella henselae neuroretinitis in cat scratch disease. diagnosis, management, and sequelae.this study aimed to report the long-term outcomes of patients treated with an antibiotic drug combination for bartonella henselae neuroretinitis.19989499776
[emerging pathologies]. 19979513170
[tropical animal and human rickettsial infections].advances in molecular biology have provided tools that have greatly clarified our knowledge of rickettsial diseases. most rickettsias have been reclassified in the alpha subgroup of proteobacteria. four groups of rickettsias have been identified: the spotted fever group, the typhus group, ehrlichia, and bartonella. although still considered as a rickettsia, coxiella burnetti, the agent causing q fever, has been reclassified separately in the gamma subdivision of proteobacteria. recognition of ri ...19979513175
absence of bartonella-like inclusions in microangiopathy after transplantation. 19989519982
[let's talk about microbes]. 19989534143
cat scratch disease in greece.an indirect fluorescent antibody test for bartonella henselae, b quintana, and b elizabethae was performed in all 18 children who presented to our paediatric outpatient clinic with cat scratch disease over a six year period. serum samples were taken on admission, after 15 days, and after six months. diagnosis was confirmed in 15 patients (83%) and was based on seroconversion or a fourfold change of the antibody titre to b henselae in 12 patients and on a single high titre (> 128) in three patien ...19989534680
cat-scratch disease in a child with unique magnetic resonance imaging findings.to report a patient with cat-scratch disease and the associated radiological findings.19989559718
a survey of tick-borne bacteria and protozoa in naturally exposed dogs from israel.antibody reactivity against seven bacterial or protozoal pathogens was measured in sera derived from 40 dogs suspected of a tick-borne disease. sera from 73% (29/40) of the dogs reacted with three or more test antigens. seroreactivity was most prevalent to babesia canis antigen (90%) followed by babesia gibsoni (75%), ehrlichia canis (63%), rickettsia conorii--moroccan strain (58%), rickettsia conorii--israeli strain no. 2 (28%), borrelia burgdorferi (10%) or bartonella vinsonii (berkhoffii) (10 ...19989561701
peloisis hepatis due to bartonella henselae in transplantation: a hemato-hepato-renal syndrome.bacillary peliosis hepatis is an uncommon but well recognized disease due to disseminated bartonella infections occurring predominantly in immunocompromised individuals infected with human immunodeficiency virus, type 1. a similar condition in the absence of bartonella infection when described in organ transplant patients was felt to be secondary to azathioprine and/or cyclosporine.19989565109
cat scratch disease presenting with peripheral facial nerve paralysis.acquired peripheral facial nerve paralysis is a relatively common disorder that affects both children and adults. the most frequent nontrauma-related etiologies in otherwise neurologically intact patients are idiopathic (bell's palsy) and infectious, which includes otitis media, herpes zoster, lyme disease, herpes simplex virus, epstein-barr virus, and mycoplasma pneumoniae. cat scratch disease (csd) is typically a subacute, regional lymphadenitis caused by bartonella henselae that is seen in ch ...19989565446
unconventional genomic organization in the alpha subgroup of the proteobacteria.pulsed-field gel electrophoresis was used to analyze the genomic organization of 16 bacteria belonging or related to the family rhizobiaceae of the alpha subgroup of the class proteobacteria. the number and sizes of replicons were determined by separating nondigested dna. hybridization of an rrn gene probe was used to distinguish between chromosomes and plasmids. members of the genus agrobacterium all possess two chromosomes, and each biovar has a specific genome size. as previously demonstrated ...19989573163
igm to bartonella henselae in cat-scratch disease and during acute epstein-barr virus infection.the diagnostic value of igm to bartonella henselae was evaluated in 20 children with cat-scratch disease (csd) and controls consisting of 20 blood donors and 20 children with enlarged lymph nodes without csd by two indirect immunofluorescence assays (ifa). one was based on b. henselae cocultivated with vero cells (host cell-associated ifa), and the other on b. henselae grown on agar (host cell-free ifa). with the host cell-associated ifa, 18 of 20 children with csd revealed igm, whereas only 14 ...19989574898
isolation of bartonella spp. from embryos and neonates of naturally infected rodents.embryos and neonatal offspring of wild-captured cotton rats (sigmodon hispidus) and white-footed mice (peromyscus leucopus) were tested for the presence of bartonella spp. isolates of bartonella spp. were obtained from 18 of 31 embryos and 7 of 19 neonates from bacteremic dams of the two species; no isolates were obtained from material from non-bacteremic dams. sequence analysis demonstrated that the isolates from embryos and neonates matched the phylogenetic group of bartonella spp. isolates ob ...19989577777
systemic cat scratch disease: a brief case report and review of the literature. 19989611416
fibrous long-spacing collagen in bacillary angiomatosis.fibrous long-spacing (fls) collagen is a distinct ultrastructural form of collagen present in normal tissue, various tumors, and tissues degraded by bacterial collagenases in vivo and in vitro. an association between fls collagen and bacillary angiomatosis has not been previously described. six cases of bacillary angiomatosis, including one autopsy case with disseminated disease, were examined ultrastructurally. in addition, kaposi sarcoma (3), pyogenic granuloma (3), capillary hemangioma (3), a ...19989615381
[aids splenomegaly and related iron problems].spleens collected from 85 consecutive autopsies of aids patients (mean age 37 years) were studied. splenomegaly, observed in 59 cases (69.4%), does not statistically correlate with life style and blood transfusions. eleven very large spleens (over 890 g) were associated with opportunistic infections (i.e.: mycobacteria, true fungi and rochalimaea). the histological pattern was characterized by marked lymphoid depletion of the white pulp and--in 67 cases (89.4%)--packing of the pulp cords by macr ...19989619056
persistent infection of pets within a household with three bartonella species.we monitored by blood culture and immunofluorescence assay (ifa) bartonella infection in one dog and eight cats in a household to determine the prevalence and persistence of the infection as well as its transmissibility to humans. ectoparasite control was rigorously exercised. during a 3-year period, bartonella clarridgeiae was recovered from one cat on two occasions, and b. henselae was isolated from another cat on four occasions. during a 16-month period, b. vinsonii subsp. berkhoffii was isol ...19989621208
culture-negative endocarditis caused by bartonella henselae.a 4-year-old girl presented with clinical evidence of infective endocarditis involving her aortic valve, but blood cultures were sterile. serologic studies and analysis of resected valve by immunohistochemistry and polymerase chain reaction established the diagnosis of bartonella henselae endocarditis. clinicians should be aware that b. henselae can cause apparent culture-negative endocarditis in children.19989627605
retinal and choroidal manifestations of cat-scratch disease.the ability to diagnose cat-scratch disease (csd) has been facilitated greatly by the recent isolation and characterization of bartonella henselae (formerly genus rochalimaea) and afipia felis and by the subsequent development of specific enzyme-linked immunosorbent assay (elisa) serologic tests. this study will help define the patterns of posterior segment ocular involvement in patients with confirmed csd.19989627652
bartonella henselae invasion of feline erythrocytes in vitro.bartonella henselae, the causative agent of cat scratch disease, establishes long-term bacteremia in cats, in which it attaches to and invades feline erythrocytes (rbc). feline rbc invasion was assessed in vitro, based on gentamicin selection for intracellular bacteria or by laser confocal microscopy and digital sectioning. invasion rates ranged from 2 to 20% of the inoculum, corresponding to infection of less than 1% of the rbc. invasion was a slow process, requiring >8 h before significant num ...19989632625
cat-scratch disease simulating histiocytosis x.cat-scratch disease is a self-limited condition commonly causing a benign chronic lymphadenopathy in children. osteolytic lesions are a rare complication, but have been previously reported. we report a case of a solitary osteolytic lesion of the skull whose clinical, radiographic and pathological features were initially interpreted as being consistent with histiocytosis x. subsequently, positive serological titers for bartonella, a history of a cat-scratch antecedent to the onset of clinical sym ...19989635688
[bartonella infections]. 19969636758
characterization of bartonella henselae isolated from bacillary angiomatosis lesions in a human immunodeficiency virus-infected patient in germany.infections with bartonella (rochalimaea) henselae can result in a variety of clinical entities, including bacillary angiomatosis in immunocompromised hosts. the fastidious nature of this bacterium has so far prevented the culture of many clinical isolates. we report the recovery of the first european b. henselae isolate associated with bacillary angiomatosis. the isolate was cultured in a frozen skin biopsy specimen from a human immunodeficiency virus (hiv)-infected patient and was characterized ...19989636850
are all bartonella henselae strains created equal? 19989636851
[liver changes in aids. retrospective analysis of 227 autopsies of hiv-positive patients].in a retrospective study of a 12-year period (1981-1992) liver histology was analyzed in 227 autopsied patients infected with the human immunodeficiency virus. normal histology could only be documented in 29 patients (13%). in the majority of cases (56%) uncharacteristic changes were seen such as steatosis (34%), hemosiderosis (10%) or non-specific reactive hepatitis (7%). the finding of hepatic peliosis obtained in 4 patients was not associated with inflammatory liver changes, especially infect ...19989648144
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