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bacillary (epithelioid) angiomatosis and concurrent kaposi's sarcoma in acquired immunodeficiency syndrome.two patients with acquired immunodeficiency syndrome developed simultaneous kaposi's sarcoma and bacillary (epithelioid) angiomatosis. the distinguishing clinical and histologic features of these two vascular proliferations associated with human immunodeficiency virus disease are described. the lesions of bacillary (epithelioid) angiomatosis contained bacteria, while the lesions of kaposi's sarcoma did not. with erythromycin therapy, the lesions of bacillary (epithelioid) angiomatosis cleared, w ...19892817918
[observations on carrión's disease]. 19892675250
bartonella bacilliformis stimulates endothelial cells in vitro and is angiogenic in vivo.bartonellosis, a biphasic disease caused by motile intracellular bacteria, produces in its tissue phase a characteristic dermal eruption (verruga peruana) resulting from a pronounced endothelial cell proliferation. bacteria are found in the interstitium and within the cytoplasm of endothelial cells (rocha-lima inclusion). the aim of this study was to determine if bartonella bacilliformis produce a substance(s) that might be responsible for the vascular proliferation seen in the verruga. this was ...19901693472
an epidemic of oroya fever in the peruvian andes.between february and october 1987, a febrile illness killed 14 persons and seriously affected at least 14 others in shumpillan, a remote peruvian mountain village of 353 people. the illness was characterized by fever, headache, chills, and pallor. the fatality rate of untreated cases was 88%. the patients, 71% of whom were male, were 1-75 years of age. fatal illnesses progressed from lethargy to coma to death in 3-60 days. patients treated empirically with chloramphenicol survived. bartonella ba ...19902316791
the agent of bacillary angiomatosis. an approach to the identification of uncultured pathogens.bacillary angiomatosis is an infectious disease causing proliferation of small blood vessels in the skin and visceral organs of patients with human immunodeficiency virus infection and other immunocompromised hosts. the agent is often visualized in tissue sections of lesions with warthin-starry staining, but the bacillus has not been successfully cultured or identified. this bacillus may also cause cat scratch disease.19902233945
a newly recognized fastidious gram-negative pathogen as a cause of fever and bacteremia.we identified a motile, curved, gram-negative bacillus as the cause of persistent fever and bacteremia in two patients with symptomatic human immunodeficiency virus infection. the same organism was subsequently recovered from a bone marrow-transplant recipient with septicemia and from two immunocompetent persons with week-long febrile illnesses. all the patients recovered after antimicrobial therapy.19902233947
blood parasites of mammals from papua new guinea.thin blood smears were collected from 126 mammals representing four genera of marsupials and six genera of murid rodents. a species of hepatozoon was discovered in the new guinea spiny bandicoot (echymipera kalubu), trypanosome infections were found in three genera of rodent hosts and the prevalence of a rickettsial parasite of the genus grahamella was recorded in rodents from the genera rattus and melomys. dried blood samples also were taken and screened serologically for antibodies to arenavir ...19902338736
transformation of rochalimaea quintana, a member of the family rickettsiaceae.rochalimaea quintana is the only member of the family rickettsiaceae that can be grown in vitro. because of its relationship to the other members of this family, techniques developed to transform r. quintana might be applicable to the obligate intracellular bacteria of the rickettsiaceae. these procedures are critical to understanding mechanisms of pathogenesis and the nature of obligate intracellular growth. a transformation procedure for r. quintana has been established by using electroporatio ...19902394681
bb65, a major immunoreactive protein of bartonella bacilliformis.a 65 kda protein (bb65) has been identified as one of the major specific antigens of bartonella bacilliformis, the causative agent of bartonellosis which is a bacterial infectious disease of inhabitants of the andes. the gene encoding this antigen (7b2) was isolated from an expression library made directly from randomly generated fragments of b. bacilliformis genomic dna using bartonella antibodies raised in rabbits and sera of bartonellosis patients. the bartonella 7b2 gene was expressed in esc ...19901700634
molecular characterization and proposal of a neotype strain for bartonella bacilliformis.bartonella bacilliformis, the etiologic agent of bartonellosis, was characterized biochemically and by dna hybridization, guanine-plus-cytosine content, genome size, and 16s rrna sequencing. dnas from the two strains in our collection exhibited 97% relatedness in hydroxyapatite reactions done at 55 degrees c (optimal reassociation criterion) and 100% relatedness in reactions done at 70 degrees c (stringent reassociation criterion). there was no evidence of divergence within the related sequences ...19911715879
16s rrna sequences of bartonella bacilliformis and cat scratch disease bacillus reveal phylogenetic relationships with the alpha-2 subgroup of the class proteobacteria.the primary structures of 16s rrnas of bartonella bacilliformis, an isolate of the cat scratch disease (csd) bacillus, and a strain phenotypically similar to the csd bacillus were determined by reverse transcriptase sequencing. these microorganisms were found to be members of the alpha-2 subgroup of the class proteobacteria. the sequence from b. bacilliformis was most closely related to the rrna of rochalimaea quintana (91.7% homology), the etiologic agent of trench fever. the sequence from the ...19911719021
genetics of rickettsiae.classical genetic approaches useful with free-living bacteria are difficult to apply to the rickettsiae. although rickettsial mutants have been isolated over the years, the genetic basis of these mutants is unknown, limiting their usefulness. the application of molecular biological techniques to rickettsial studies has provided the opportunity to isolate and study specific genes. genes encoding metabolic enzymes from rickettsiae were cloned in escherichia coli and shown to retain their regulator ...19911679397
transformation and genomic restriction mapping of rochalimaea spp.transformation procedures using electroporation were established for rochalimaea quintana. several cosmid/plasmids possessing the rk2 or rsf1010 origin of replication were successfully inserted. plasmid retention and replication were verified by antibiotic resistance and southern blot analysis. the highest level of transformation was obtained at a voltage field strength of 12.5 kv/cm with a pulse time of 10 miliseconds. transformation efficiency was low (0.3%) with approximately 10(5) transforma ...19911687634
daniel carrión and carrión's disease. 19911742572
taxonomic considerations of bartonella bacilliformis based on phylogenetic and phenotypic characteristics.the 16s-rrna gene of bartonella bacilliformis was amplified using the polymerase-chain reaction (pcr). the amplification product was sequenced using a linear-pcr procedure and compared with other published 16s-rrna sequences. the results of this analysis placed b. bacilliformis in the alpha subgroup of the proteobacteria, and more specifically demonstrated its close phylogenetic relationship to rochalimaea quintana. this relationship is supported by similarities in the size and mean base composi ...19911778432
the causative agent of bacillary angiomatosis. 19911922261
bartonellosis. an immunodepressive disease and the life of daniel alcides carrión.the history of human bartonellosis, a unique south american biphasic bacterial disease, is reviewed. the course of this disease and its natural history offer many puzzling problems. its geographic distribution, its absence of a natural reservoir, the development of transient immunosuppression during the acute hematic phase, and the subsequent development of vascular proliferations are highlighted. these vascular proliferations represent a unique example of tissue reactivity recalling the vascula ...19912008885
bacillary angiomatosis: a systemic opportunistic infection with prominent cutaneous manifestations.bacillary angiomatosis is an opportunistic infection with systemic manifestations. although most cases have occurred in human immunodeficiency virus-positive patients, other immunosuppressed patients, and even seemingly immunocompetent individuals, can become infected. in addition to the well-characterized cutaneous manifestations, visceral involvement can occur and may be the only locus of infection. lymphadenopathy, bone or soft-tissue masses, fever, and hepatosplenomegaly can be presenting si ...19911931568
clinical, histologic, microbiologic, and biochemical characterization of the causative agent of bacillary (epithelioid) angiomatosis: a rickettsial illness with features of bartonellosis.it has been suggested that bacillary (epithelioid) angiomatosis (bea) is a manifestation of cat scratch disease (csd). because of clinical similarity between this condition and the verruga peruana phase of bartonellosis, we sought to further characterize this disease as well as its causative agent and to compare it to bartonellosis. we isolated a small flagellated pleomorphic bacillus from skin lesions of two patients with bea. organisms were stained successfully with warthin-starry silver stain ...19911919046
cat-scratch disease and bacillary angiomatosis. 19911895469
splenomegaly and reticulocytosis caused by babesia microti infections in natural populations of the montane vole, microtus montanus.a survey for babesia microti in rodents was conducted at six sites within grand teton national park, wyoming. blood and spleen smears, hematocrits, and reticulocyte counts were made on all of the animals to evaluate parameters for the diagnosis of babesiosis. ticks were removed for identification. of 257 microtus montanus, 103 were infected with b. microti. in addition, five of 12 microtus pennsylvanicus and one of three arvicola richardsoni were parasitized by b. microti. peromyscus maniculatus ...19911818201
[a 60-year-old patient with recurrent angiomatous lesions]. 19921598432
dermatopathologic findings in patients infected with hiv.several inflammatory, infectious, and neoplastic conditions in hiv-infected patients are distinctive or require a biopsy for diagnosis. some differ subtly from similar conditions seen in noninfected patients. the exanthem of acute hiv infection cannot be diagnosed specifically on biopsy as its histologic appearance is similar to that of other viral exanthemata. a condition that closely resembles seborrheic dermatitis occurs in hiv-infected patients. plasma cells, necrotic keratinocytes, and leuk ...19921730173
dendrocytes in verruga peruana and bacillary angiomatosis.verruga peruana and bacillary angiomatosis are two cutaneous diseases characterized by angiomatous growths linked to the presence of rickettsia-like organisms. these lesions are currently considered to be endothelial hyperplasias and to share many features. by immunohistochemistry and computerized image analysis, we studied the presence of factor-xiiia-positive dendrocytes in these lesions and compared our data with similar research in capillary angiomas and normal skin. other cell lines were st ...19921348435
serological response to "rochalimaea henselae" antigen in suspected cat-scratch disease.there are no generally accepted diagnostic tests for cat-scratch disease (csd), the cause of which is unknown. during the development of an indirect fluorescence antibody (ifa) test for detection of antibodies to "rochalimaea henselae", sera from patients with csd were found to have high titres to r henselae antigens. further tests with this assay showed that 36 (88%) of 41 patients with suspected csd had serum titres of 64 or more to r henselae antigen, that there was a low prevalence (3%) of s ...19921351130
naturally occurring "rochalimaea henselae" infection in domestic cat. 19921354314
possible role of rochalimaea henselae in pathogenesis of aids encephalopathy. 19921357365
characterization of a novel rochalimaea species, r. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient.isolation of a rochalimaea-like organism from a febrile patient infected with human immunodeficiency virus was confirmed. analysis of 16s rrna gene sequences, together with polymerase chain reaction and restriction endonuclease length polymorphism analysis of a portion of the citrate synthase gene, demonstrated that the agent is closely related to members of the genus rochalimaea and that the isolate is genotypically identical to the presumptive etiologic agent of bacillary angiomatosis. however ...19921371515
phylogenetic relationships among the agent of bacillary angiomatosis, bartonella bacilliformis, and other alpha-proteobacteria.bacillary angiomatosis (ba) and chronic bartonellosis are bacterial infections of humans which result in an unusual vascular proliferative tissue response. in order to determine their phylogenetic relationships, we have determined greater than 95% of the 16s rrna sequences for these two organisms by amplification directly from infected ba tissue and from a bartonella bacilliformis lyophilized culture. the ba agent and b. bacilliformis are closely related alpha-proteobacteria (98.5%), although th ...19921378524
identification of the rochalimaea henselae 16s rrna sequence in the liver of a french patient with bacillary peliosis hepatis. 19921385550
nuclear planimetry and dna flow cytometry of verruga peruana.verruga peruana is a vascular hyperplasia that is sometimes histologically mistaken for a malignant neoplasm. an infiltrative pattern with nuclear pleomorphism and mitoses is prominent in some lesions. from a series of lesions of verruga peruana, we selected five atypical lesions, two of them closely resembling epithelioid angiosarcoma. we used histologic criteria as well as morphometry and flow cytometry to further characterize the atypical features of the lesions. histology and morphometry evi ...19921388574
adhesion to and invasion of cultured human cells by bartonella bacilliformis.bartonella bacilliformis was tested for its ability to adhere to and invade tissue culture cell monolayers. the parasite was able to efficiently bind and penetrate human dermal fibroblasts, human laryngeal epithelium, and human umbilical vein endothelial cells. exposure of the organism to immune serum prepared against a crude bartonella extract containing cell wall and membranous material resulted in decreased ability of the parasite to invade host cells. there was also an overall reduction in t ...19921398917
a phage in bartonella bacilliformis.bacteriophage-like particles were found in bartonella bacilliformis culture. the particles consisted of head (icosahedral), 40 nm in diameter, and tail, 16 nm in length.19921406375
isolation of rochalimaea species from cutaneous and osseous lesions of bacillary angiomatosis.bacillary angiomatosis is characterized by vascular lesions, which occur usually in patients infected with the human immunodeficiency virus (hiv). a newly described gram-negative organism, rochalimaea henselae, has been associated with cutaneous bacillary angiomatosis, but no organism has been isolated and cultivated directly from cutaneous tissue.19921435899
infections due to rochalimaea: the expanding clinical spectrum. 19921457660
the causative agent of bacillary angiomatosis. 19921459755
detection of bartonella bacilliformis in cultures, blood, and formalin preserved skin biopsies by use of the polymerase chain reaction.a polymerase chain reaction (pcr) is described for the detection of bartonella bacilliformis, the etiologic agent of bartonellosis, which cannot be identified biochemically. amplification of a genomic 231 bp bartonella dna sequence permitted specific identification of 12 bartonella isolates from peruvian bartonellosis patients as well as detection of bartonella dna in blood samples and formaldehyde preserved skin biopsies. specificity of amplification products was confirmed by restriction fragme ...19921470840
immunocytochemical identification of rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia.we report the immunocytochemical identification of rochalimaea henselae, a newly recognized fastidious, gram-negative, warthin-starry-positive organism, as the common pathogen in bacillary angiomatosis (ba), bacillary peliosis (bp) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. immunogenic proteins of the r. henselae strain isolated from the blood of a febrile immunocompromised patient with bp of the liver were used to produce primary immune serum in ...19921530106
rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis.nine strains of rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and dna interrelatedness among rochalimaea spp., bartonella bacilliformis, and afipia felis (cat scratch disease bacillus). all except one isolate, which was rochalimaea quintana, were determined to belong to a newly proposed species, rochalimaea henselae sp. nov. after recovery from clinical material, colonies required 5 t ...19921537892
deformation factor: an extracellular protein synthesized by bartonella bacilliformis that deforms erythrocyte membranes.bartonella bacilliformis, a hemotropic bacterium and the causative agent of the human disease bartonellosis, when incubated in a tryptone-based medium produces an extracellular factor, termed deformation factor (df), which induces extensive indentations and trenches in trypsinized erythrocyte membranes. the factor is stable during storage at 4 degrees c. it can be inactivated by proteases or brief heating to 70 to 80 degrees c, can be precipitated by ammonium sulfate, is nondialyzable, and is re ...19921541567
rochalimaea henselae causes bacillary angiomatosis and peliosis hepatis.recent studies have demonstrated that a newly described agent of persistent bacteremia, rochalimaea henselae, and the agent of bacillary angiomatosis are both closely related to rochalimaea quintana. bacillary peliosis hepatis seemed likely to have the same etiologic agent as bacillary angiomatosis. we sought these pathologic changes in patients from whom r henselae was cultivated.19921546924
relapsing illness due to rochalimaea henselae in immunocompetent hosts: implication for therapy and new epidemiological associations.two previously healthy, immunocompetent men had persistent rochalimaea henselae bacteremia with clinical relapses after courses of antibiotics to which the isolates were ultimately demonstrated susceptible in vitro. both had sustained tick bites prior to their illnesses, thus demonstrating an association not previously identified, although suspected. the first patient had relapsing fever, constitutional symptoms, and an episode of aseptic meningitis despite therapy with amoxicillin, then with do ...19921562660
bacillary angiomatosis. a "new" disease with a broadening clinicopathologic spectrum.bacillary angiomatosis (ba) is a reactive vasoproliferative lesion occurring almost exclusively in immunocompromised individuals in response to infection by a bacillus closely related to rochalimaea quintana. the commonest site of involvement is the skin, in the form of multiple erythematous nodules, but bacillary angiomatosis can also present in a wide variety of sites such as soft tissues, bone, lymph node, liver and spleen. some patients may present with persistent fever and bacteraemia. baci ...19921576430
interactions between live bartonella bacilliformis and endothelial cells.bartonella bacilliformis, a gram-negative, flagellated, motile bacterium, is the etiologic agent of verruca peruana. it is found within the verruca, where it can form large cytoplasmic (rocha-lima) inclusions in endothelial cells. previously, an activity has been described in homogenates of b. bacilliformis that in vitro increases the proliferation of endothelial cells and their production of tissue-type plasminogen activator (t-pa) and in vivo is angiogenic. the aim of the present study was to ...19921583335
murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana.rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ...19921629326
nucleotide sequence and comparison of the 5s ribosomal rna genes of rochalimaea henselae, r.quintana and brucella abortus. 19937685083
characterization of the fmet initiator trna gene of bartonella bacilliformis.a ribosomal rna operon was cloned from the agent of human oroya fever, bartonella bacilliformis. the 3' end of the operon contains a 77-nucleotide (nt) n-formylmethionine (fmet) initiator trna-encoding gene with 100% sequence identity to the fmet trna-encoding gene of rhodobacter sphaeroides. the 3' region downstream from the gene has an 8-nt inverted repeat and 28 contiguous direct repeats of the heptamer 5'-ttctcta. to date, b. bacilliformis and r. sphaeroides are the only known eubacteria whi ...19937690338
cat scratch disease and bacillary angiomatosis: aetiological agents and the link with aids.the recent characterisation of a new species of the genus rochalimaea is the culmination of a ten year search for the identity of the agent of bacillary angiomatosis, a condition that is increasingly recognised in association with aids. this work has also implicated rochalimaea henselae in the aetiology of cat scratch disease contact with cats is a risk factor for both conditions. this report describes both diseases and reviews progress toward understanding their microbiology. r. quintana is als ...19937693166
proposed tests for the routine identification of rochalimaea species.a study was conducted to establish tests for the routine identification of rochalimaea species. strains used were reference strains of rochalimaea vinsonii and rochalimaea quintana, and a type strain and six human isolates of rochalimaea henselae. rochalimaea species were confirmed to be gram-negative, oxidase-negative, non-motile, urease-negative, indole-negative, catalase-negative, glucose-nonfermenting organisms which failed to grow on macconkey agar. further testing of the organisms in a com ...19937694852
rochalimaea elizabethae sp. nov. isolated from a patient with endocarditis.a rochalimaea-like organism (strain f9251) was isolated from a patient with endocarditis after blood drawn for culture before antimicrobial therapy was subcultured onto blood and chocolate agars and incubated for 2 weeks in 5% co2. the strain was phenotypically similar to known rochalimaea species. the cellular fatty acid composition of strain f9251 was close to but distinct from those of the three known rochalimaea species and was most similar to that of r. vinsonii. labeled dna from strain f92 ...19937681847
phylogenetic analysis of the family rhizobiaceae and related bacteria by sequencing of 16s rrna gene using pcr and dna sequencer.the 16s rrna gene sequences of 19 strains covering 97% of the molecules were determined for the members of the family rhizobiaceae and related bacteria by pcr and dna sequencer. the three biovars of agrobacterium were located separately, whereas agrobacterium rubi clustered with a. tumefaciens. phylogenetic locations for the species of the genera rhizobium, sinorhizobium, agrobacterium, phylobacterium, mycoplana (m. dimorpha), ochrobactrum, brucella and rochalimaea (a rickettsia) were intermingl ...19937682191
[geographic distribution of lutzomyia verrucarum (townsend, 1913) (diptera, psychodidae, phlebotominae), vector of human bartonellosis in peru].lutzomyia verrucarum (townsend, 1913) (diptera: psychodidae); the natural vector of bartonella bacilliformis, agent of human bartonellosis (peruvian verruga or carrion's disease), is a native species of peru; its geographic distribution occurrs between latitudes 5 degrees and 13 degrees 25' south: in the occidental and interandean valleys of the andean. the altitudinal distribution of lu. verrucarum in the different valleys is as follows: occidental between 1100 and 2980 m sea level and interand ...19937997750
antimicrobial susceptibility of rochalimaea quintana, rochalimaea vinsonii, and the newly recognized rochalimaea henselae.the susceptibility of an isolate of rochalimaea quintana, rochalimaea vinsonii and the newly recognized rochalimaea henselae to antimicrobial agents was determined by dilution in mueller-hinton agar supplemented with 5% sheep blood. two inocula of 10(2) and 10(4) cfu were used and tests were read after incubation at 5 and 7 days at 37 degrees c in an atmosphere enriched to 5% carbon dioxide. each isolate was susceptible to amoxycillin, third-generation cephalosporins, tetracyclines, macrolides a ...19938288500
proposals to unify the genera bartonella and rochalimaea, with descriptions of bartonella quintana comb. nov., bartonella vinsonii comb. nov., bartonella henselae comb. nov., and bartonella elizabethae comb. nov., and to remove the family bartonellaceae from the order rickettsiales.dna hybridization data (hydroxyapatite method, 50 to 70 degrees c) indicate that rickettsia prowazekii, the type species of the type genus of the family rickettsiaceae, is substantially less closely related to rochalimaea species than was previously thought. the levels of relatedness of rickettsia prowazekii to rochalimaea species and to bartonella bacilliformis under optimal conditions for dna reassociation were 0 to 14%, with 25.5% or greater divergence in related sequences. when stringent rea ...19938240958
bacteremia due to rochalimaea henselae in a child: practical identification of isolates in the clinical laboratory.two closely related species of rochalimaea, rochalimaea quintana and rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. we report a case of culture-proven r. henselae bacteremia in a child with persistent fever. serologic evidence of infection by r. henselae was ascertained by testing sera at two intervals for immunoglobulin g or immunoglobulin m antibodies by enzyme immunoassay and immun ...19938408560
the epidemiology of bacillary angiomatosis and bacillary peliosis.to determine environmental risk factors for bacillary angiomatosis-bacillary peliosis (bap), and to confirm infection with rochalimaea species.19938423659
syndrome of rochalimaea henselae adenitis suggesting cat scratch disease.to describe a clinical syndrome of cat scratch disease caused by rochalimaea henselae, including methods for isolation of the organism from tissue and for identification.19938430978
endocarditis caused by rochalimaea quintana in a patient infected with human immunodeficiency virus.rochalimaea quintana and rochalimaea henselae are closely related, fastidious, gram-negative rickettsiae. thus far, the spectrum of human rochalimaea sp. infections has not included endocarditis. we describe a 50-year-old human immunodeficiency virus-positive man who developed endocarditis caused by r. quintana. dna relatedness studies, which compared our patient's blood culture isolate with known rochalimaea species, identified the organism as r. quintana. our report expands the spectrum of roc ...19938458964
eia for detection of rochalimaea henselae-reactive igg, igm, and iga antibodies in patients with suspected cat-scratch disease. 19938501350
cat scratch disease in connecticut. epidemiology, risk factors, and evaluation of a new diagnostic test.although cat scratch disease is commonly diagnosed in patients who have unexplained regional lymphadenopathy after encounters with cats, its epidemiology and the risk factors for disease are not clearly defined, and there is no generally accepted diagnostic test.19938505963
polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from rochalimaea species for subtyping.restriction endonuclease analysis of a polymerase chain reaction-amplified dna fragment which included the spacer region between the genes coding for 16s and 23s rrnas and a portion of the gene coding for 23s rrna (spacer + 23s) was done on 10 previously characterized clinical isolates of rochalimaea henselae, one clinical isolate of rochalimaea quintana, and the type strains of r. henselae, r. quintana, rochalimaea vinsonii, and bartonella bacilliformis. brucella abortus dna was not amplified b ...19938102375
bacillary angiomatosis of the spleen.bacillary angiomatosis is a recently described vasoproliferative lesion associated with infection by a newly characterized rickettsial organism, rochalimaea henselae. most previous reports have described skin lesions in immunocompromised patients infected with human immunodeficiency virus. this is the first case report detailing the features of bacillary angiomatosis of the spleen occurring in a patient undergoing cytotoxic chemotherapy for disseminated ovarian carcinoma.19938165007
[bacillary angiomatosis. a pseudoneoplastic infection in aids patients].bacillary angiomatosis is a newly recognized pseudoneoplastic vascular disease seen in the setting of human immunodeficiency virus (hiv) infection. the disease is characterized by a cutaneous infection with reddish papules or nodules that are similar to pyogenic granulomas or kaposi's sarcoma in clinical appearance. it is caused by the mildly gram-negative bacillus rochalimaea henselae, which can be identified in tissue sections by means of warthin-starry stain. the historical, clinical, histopa ...19938335458
cat scratch disease and rochalimaea henselae. 19938336627
development and evaluation of a blood-free medium for determining growth curves and optimizing growth of rochalimaea henselae.two strains of rochalimaea henselae were used to optimize a blood-free growth medium. seven agar bases, four broths, and combinations of eight supplements were evaluated. acceptable growth was achieved in media containing fildes solution and hemin, with the best growth demonstrated in brucella broth or on brucella agar with 6 to 8% fildes solution and 250 micrograms of hemin per ml. r. henselae utilized hemin in concentrations six times that utilized by rochalimaea quintana. erythrocyte membrane ...19938349767
rochalimaea infections. 19938357124
detection of rochalimaea henselae in cat-scratch disease skin test antigens.one of the diagnostic criteria for cat-scratch disease is a positive delayed-type hypersensitivity skin test reaction to an antigen prepared from purulent material aspirated from a cat-scratch disease-involved lymph node. polymerase chain reaction coupled with dna sequence analysis was used to identify organisms found in these skin test antigen preparations that may be responsible for eliciting this response. two independent sources of cat-scratch disease skin test antigens yielded dna sequences ...19938376816
association between rochalimaea infection and cat-scratch disease. 19938399887
nucleotide sequence of the 5s ribosomal rna gene of bartonella bacilliformis. 19937680804
isolation of rochalimaea species. 19937682656
[bacillary peliosis in aids. anatomo-clinical study of 2 cases].the authors report two cases of peliosis hepatis, occurring in patients with aids, who presented a persistent fever and an hepatomegaly. the liver biopsies showed areas of peliosis, where bacilli were observed by warthin-starry stain. in one case, techniques of molecular biology allowed the identification of rochalimaea henselae, pathogen involved in bacillary angiomatosis. this rickettsia has been newly recognized in the united-states, where 17 cases of bacillary peliosis have been published in ...19938311862
characterization of bartonella bacilliformis flagella and effect of antiflagellin antibodies on invasion of human erythrocytes.bartonella bacilliformis is the etiologic agent of oroya fever in humans. flagellum-mediated motility has been postulated as a major virulence factor for invasion of host cells. to address this hypothesis, we purified and characterized flagella from strain kc584 and then assessed their role in human erythrocyte association and invasion. electron microscopy of the flagellar preparation showed a high concentration of filaments with a mean wavelength of 800 nm. sodium dodecyl sulfate-polyacrylamide ...19938225570
ehrlichia chaffeensis and rochalimaea antibodies in kawasaki disease.sera from 38 patients with kawasaki disease were tested for immunofluorescent antibodies to ehrlichia chaffeensis, rochalimaea henselae, and r. quintana oklahoma. only 2.5% of the patients tested positive for e. chaffeensis, and 5% were positive for r. henselae and r. quintana oklahoma. our data suggest that ehrlichia and rochalimaea spp. do not play a unique role in the etiology of kawasaki disease.19938263201
cat-scratch disease mimicking rhabdomyosarcoma.a 12-year-old boy who had injured his elbow presented with a lump in the epitrochlear area. originally thought to be a hematoma, then later myositis ossificans, this mass was actually a lymph node with large, reactive germinal centers. although stains for acid-fast bacilli, fungal organisms, bacteria, and cat-scratch disease were negative, serological testing for detection of rochalimaea henselae organisms found evidence of cat-scratch disease. we would like to remind orthopaedic surgeons to con ...19938265222
[bacillary angiomatosis of the oral cavity in aids. a differential diagnosis of mucosal kaposi's sarcoma].the diagnosis of bacillary angiomatosis (ba) was performed on a biopsy of a reddish nodular lesion of the gingival mucosa, in a patient seropositive for the h.i.v. it was based on the histopathological features, and by the demonstration of bacilli both on the warthin-starry stain and the electron microscopic study. a treatment by erythromycin was quickly effective. ba is an opportunistic infection seen essentially in patients with a.i.d.s., most often causing cutaneous involvement. the causative ...19938278795
hepatosplenic cat-scratch disease associated with elevated anti-rochalimaea antibody titers. 19938284124
endocarditis caused by rochalimaea henselae.a case of community-acquired, culture-negative, infective endocarditis was diagnosed in a 57-year-old construction worker. small, pleomorphic gram-negative rods were seen in brown-hopps tissue gram stains and warthin-starry silver stains. the organism was identified as rochalimaea henselae by polymerase chain reaction amplification and sequencing of the 16s rdna gene sequence. this is the first report of infective endocarditis caused by r henselae.19938406424
[rochalimaea henselae infection. febrile pancytopenia and hepatic peliosis in a patient with hiv infection].a case of rochalimaea henselae infection in an aids patient is reported. the r. henselae infection was revealed by febrile pancytopenia associated with liver and spleen enlargement. the diagnosis was made on the finding at histology of hepatic peliosis lesions. within these lesions warthin starry staining displayed bacilli that were identified as r. henselae. this case shows that all hiv-infected patients with these symptoms might be infected with this organism.19938511079
morphologically variable bacilli of cat scratch disease are identified by immunocytochemical labeling with antibodies to rochalimaea henselae.the identification of the causative organisms of cat scratch disease (csd) has been elusive. the demonstration of warthin-starry stain-positive pleomorphic bacilli in lymph nodes of patients with csd and recent serologic and epidemiologic data suggest an etiologic role of rochalimaea henselae in csd. the authors studied lymph node biopsy specimens of 46 patients with illnesses clinically consistent with csd and found pleomorphic bacilli in 15 (33%). the organisms were labeled by polyclonal rabbi ...19948178768
disseminated cat-scratch disease: detection of rochalimaea henselae in affected tissue.an immunocompetent 9-year-old boy with disseminated cat scratch disease involving spleen, cervical and abdominal lymph nodes, skull, and one clavicle is reported. antibodies to rochalimaea quintana and r. henselae were detected, at increasing, then decreasing concentration. dna extracted from the biopsied skull lesion was amplified by polymerase chain reaction and hybridized with species-specific oligonucleotides proving the presence of r. henselae in affected tissue. our findings suggest that r ...19948313920
transformation of bartonella bacilliformis by electroporation.bartonella bacilliformis is a member of the order rickettsiales, family bartonellaceae. the bacterium is an intracellular parasite of human erythrocytes. to date, members of the family bartonellaceae have not been transformed by standard chemical methods. we report the first successful transformation of a member of the bartonellaceae family, b. bacilliformis, by the method of electroporation. the optimal conditions for electroporation of b. bacilliformis include a field strength of 12.5 kv/cm an ...19947954111
[recognition and identification of unknown infectious agents].the recognition and the identification of previously unrecognized infectious agents require a multidisciplinary approach to specify the nosologic entity of the disease and the epidemiological data, especially the modes of transmission and the risk factors, as well as to discover the microorganism in the laboratory. in the past 20 years, significant breakthroughs have been achieved in cellular cultures (growth factors), in immunology (monoclonal antibodies), and moreover in molecular biology, whi ...19947939294
[cat-scratch disease and bacillary angiomatosis. an old and a new infectious disease with common etiology?].a review of cat-scratch disease (csd) and bacillary angiomatosis (ba) is presented on the basis of published articles. two newly identified bacteria--rochalimaea henselae and afipia felis--have been isolated from patients with csd. preliminary investigations seem to indicate that a. felis is an uncommon cause of the disease. csd may appear as a local suppurative lymphadenopathy or a systemic infection. ba is caused by rochalimaea species and may appear as cutaneous, mucous or visceral angiomas o ...19947998352
rochalimaea antibodies in hiv-associated neurologic disease.rochalimaea henselae, a recently described pathogen thought to cause syndromes as varied as bacillary angiomatosis, parenchymal bacillary peliosis, fever with bacteremia, and cat-scratch disease, is associated with cns diseases including cerebral and retinal bacillary angiomatosis, as well as cat-scratch-related encephalitis, myelitis, cerebral arteritis, and retinitis. we used a newly developed enzyme immunoassay and the polymerase chain reaction to investigate the association of r henselae inf ...19948035937
ophthalmic manifestations of rochalimaea species.rochalimaea henselae and r. quintana belong to the order rickettsiales and are thought to be responsible for trench fever, bacillary angiomatosis, and cat scratch disease. we recently examined four patients with intraocular inflammation of unknown origin. each patient had either unilateral or bilateral moderate loss of visual acuity ranging from 20/25 to counting fingers. bilateral intraocular inflammation manifested by anterior and posterior segment cells, retinal lesions, macular exudate, and ...19948053459
bartonella bacilliformis or a similar organism and cardiovascular disease.bartonella bacilliformis invades the endothelial lining of the cardiovascular system. damage to the red blood cells and white blood cells, the effects of the toxins, invasion of the brain and electrical charges induced by the organism so interfering with normal electrical stimulation of the heart may explain many of the features of cardiovascular disease (1-5).19947815963
short report: verruga peruana in an italian traveler from peru.we report a case of cutaneous bartonellosis in a 24-year-old italian woman. soon after returning from a seven-month stay in a central area of peru at an altitude of 3,130 meters, she developed high fever, headache, and muscle and joint pain that resolved after antibiotic therapy. two months later, she presented with disseminated, painless, firm, purple, subcutaneous nodules on the neck, shoulders, legs, left eyelid, and hard palate, some of which developed into vegetating lesions. the diagnosis ...19948116804
rochalimaea henselae infections: newly recognized zoonoses transmitted by domestic cats. 19948144389
rochalimaea henselae organisms possess an elevated capacity of binding to peripheral blood lymphocytes from patients with cat scratch disease.cat scratch disease (csd) is a clinical condition whose aetiological agent, according to recent findings, is of bacterial origin. two gram-negative bacteria are invoked as causative agents of csd, namely afipia felis and rochalimaea henselae. in this paper, five patients with suspected csd were studied in terms of binding capacity of a. felis and r. henselae to their own peripheral blood lymphocytes (pbl). this parameter was correlated with serum antibody titres to both a. felis and r. henselae, ...19948152395
cat scratch disease, bacillary angiomatosis, and other infections due to rochalimaea. 19948164704
identification of outer membrane proteins of bartonella bacilliformis.purification of the outer membrane of bartonella bacilliformis by sucrose step gradient centrifugation and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) suggest that 14 proteins, ranging from 11.2 to 75.3 kda, are located in the outer membrane of the pathogen. on the basis of m(r)s, eleven of these proteins have counterparts which are labeled by extrinsic radioiodination of intact bartonellae, and two of the proteins are visibly sensitive to extrinsic proteinas ...19948188391
histological and ultrastructural study of one case of oral bacillary angiomatosis in hiv disease and review of the literature.bacillary angiomatosis (ba) is a new clinicopathological entity defined as a pseudo-neoplastic capillary proliferation secondary to an opportunistic infection by one of two rochalimaea sp.: r. quintana or r. henselae. although ba is a recently recognised entity, numerous cases have been reported. most of the patients affected are reported to have low absolute cd4 lymphocyte counts associated with aids. yet, very few oral cases associated or not with cutaneous lesions have been reported or simply ...19949135977
atypical cat-scratch disease: diagnosis by a serologic test for rochalimaea species.three patients with atypical manifestations of cat-scratch disease (csd) had the diagnosis supported by a newly available serologic assay for antibodies to rochalimaea henselae. previously, csd has been a diagnosis of exclusion because no confirmatory test was readily available. atypical or severe cases of csd have often required detailed (and expensive) clinical and laboratory investigation. the use of rochalimaea-specific serologic tests may help avoid extensive diagnostic testing or invasive ...19948153776
[vascular tumors of the skin and soft tissue. overview of newly characterized entities and variants].this review summarizes vascular tumours of skin and soft tissues that have been characterised in recent years. although most of them are very rare, knowledge about their reproducible clinicopathological features is important to avoid diagnostic pitfalls. these lesions include: bacillary angiomatosis, a vasoproliferative, pseudoneoplastic infection of immunocompromised patients caused by rochalimaea henselae; tufted angioma, a variant of lobular capillary haemangioma characterised by a "cannon-ba ...19947824435
the cat scratch disease-bacillary angiomatosis puzzle. 19948053471
animal-associated opportunistic infections among persons infected with the human immunodeficiency virus.a number of animal-associated infections occur in persons infected with the human immunodeficiency virus (hiv), including those due to toxoplasma gondii, cryptosporidium, microsporida, salmonella, campylo-bacter, giardia, rhodococcus equi, rochalimaea, and listeria monocytogenes. most of these infections, with the exception of those due to rochalimaea, appear to be acquired by the immunosuppressed individual from sources other than exposure to animals. drs. glaser and colleagues review our curre ...19948054433
epidemiologic observations on infection with rochalimaea species among cats living in baltimore, md.cats from several sources in baltimore, md, were tested for seropositivity to rochalimaea henselae and r quintana. co-infection with toxoplasma gondii or feline immunodeficiency virus was assessed as a risk factor for infection with rochalimaea spp. of 592 cats tested, 87 (14.7%) were seropositive for one or both rochalimaea spp, although titers to r henselae were significantly higher than those to r quintana. prevalence of seropositivity increased significantly with cat age and weight and was a ...19948063598
bacillary angiomatosis: a new entity in acquired immunodeficiency syndrome.since the recognition of the acquired immunodeficiency syndrome (aids) in 1981, previously rare infections and neoplasms have become increasingly common. bacillary angiomatosis, undescribed in the medical literature prior to 1983, is now second in frequency only to kaposi's sarcoma with respect to the cutaneous manifestations associated with human immunodeficiency virus (hiv) infection. caused by rochalimaea henselae, bacillary angiomatosis is easily treated, when diagnosed early, with erythromy ...19948064965
bacillary angiomatosis and rochalimaea species. 19948086116
taxonomic position of the rickettsiae: current knowledge.the term rickettsiae initially encompassed all intracellular bacteria. early rickettsial taxonomy was based on a comparison of a few phenotypic characteristics and recently, molecular studies brought new bases for rickettsial taxonomy. all rickettsial species studied so far belong to the alpha and gamma groups of the proteobacteria. ehrlichiae complex groups cowdria ruminantium, anaplasma marginale and wolbachia pipientis and the related parthenogenesis and cytoplasmic incompatibility bacteria, ...19948117465
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