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[cat-scratch disease and bacillary angiomatosis. an old and a new infectious disease with common etiology?].a review of cat-scratch disease (csd) and bacillary angiomatosis (ba) is presented on the basis of published articles. two newly identified bacteria--rochalimaea henselae and afipia felis--have been isolated from patients with csd. preliminary investigations seem to indicate that a. felis is an uncommon cause of the disease. csd may appear as a local suppurative lymphadenopathy or a systemic infection. ba is caused by rochalimaea species and may appear as cutaneous, mucous or visceral angiomas o ...19947998352
histopathological and electron microscopical features of skin lesions in a patient with bartonellosis (verruga peruana).chronic verruga nodules taken from a patient with bartonellosis (verruga peruana) were studied. histologically, specimens of all the verruga nodules had features consistent with granulomatous lesions with extensive infiltration of various types of cells along with the proliferation of capillaries. the sections were predominantly infiltrated with neutrophils and endothelial cells; histiocytes, plasma cells, lymphocytes and mast cells were also visible to some extent. the blood vessels were dilate ...19948014271
identification and characterization of a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis.a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. the gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kda in m. a. paratuberculosis. the clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative se ...19947921248
rochalimaea antibodies in hiv-associated neurologic disease.rochalimaea henselae, a recently described pathogen thought to cause syndromes as varied as bacillary angiomatosis, parenchymal bacillary peliosis, fever with bacteremia, and cat-scratch disease, is associated with cns diseases including cerebral and retinal bacillary angiomatosis, as well as cat-scratch-related encephalitis, myelitis, cerebral arteritis, and retinitis. we used a newly developed enzyme immunoassay and the polymerase chain reaction to investigate the association of r henselae inf ...19948035937
ophthalmic manifestations of rochalimaea species.rochalimaea henselae and r. quintana belong to the order rickettsiales and are thought to be responsible for trench fever, bacillary angiomatosis, and cat scratch disease. we recently examined four patients with intraocular inflammation of unknown origin. each patient had either unilateral or bilateral moderate loss of visual acuity ranging from 20/25 to counting fingers. bilateral intraocular inflammation manifested by anterior and posterior segment cells, retinal lesions, macular exudate, and ...19948053459
the cat scratch disease-bacillary angiomatosis puzzle. 19948053471
conjunctival swab to diagnose ocular cat scratch disease. 19948053472
the 16s-23s rrna intergenic spacer region of bartonella (rochalimaea) species is longer than usually described in other bacteria.we amplified by polymerase chain reaction (pcr) and sequenced using an automated laser fluorescent dna sequencer (pharmacia) the intergenic spacer region (its) between the 16s and 23s rrnas of the four species of rochalimaea which were recently renamed bartonella sp. we obtained dna fragments of 1211, 1262, 1258 and 1529 bp for the reference species of b. quintana, b. henselae, b. vinsonii and b. elizabethae, respectively. the its of the four species are longer than previously reported in prokar ...19957737501
seroepidemiological survey of bartonella (rochalimaea) henselae in domestic cats in japan.a total of 199 domestic cat serum samples from 3 geographical areas (northeastern, central and southwestern) of japan collected between 1992 and 1994 were examined for serum antibody against bartonella henselae using an immunofluorescent assay. the antibody prevalence was 15.1% (30/199). a significant difference in the prevalence of b. henselae antibody was observed between the northeastern area (6.3%:3/48) and the central area (22.0:13/59) in japan. there was no significant difference between t ...19957565174
detection of rochalimaea henselae dna in specimens from cat scratch disease patients by pcr.a pcr assay was developed by using degenerate primers that allow amplification of a 414-bp fragment of dna from the rickettsia-like organisms rochalimaea henselae and r. quintana. internal oligonucleotides were used as hybridization probes, permitting rapid differentiation of these two rochalimaea species. dnas from 12 different isolates of r. henselae were amplified with the pcr primers, and the resulting 414-bp pcr product hybridized only with the r. henselae-specific probe. dnas from four dif ...19948027347
bacillary angiomatosis and rochalimaea species. 19948086116
polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from rochalimaea species for subtyping.restriction endonuclease analysis of a polymerase chain reaction-amplified dna fragment which included the spacer region between the genes coding for 16s and 23s rrnas and a portion of the gene coding for 23s rrna (spacer + 23s) was done on 10 previously characterized clinical isolates of rochalimaea henselae, one clinical isolate of rochalimaea quintana, and the type strains of r. henselae, r. quintana, rochalimaea vinsonii, and bartonella bacilliformis. brucella abortus dna was not amplified b ...19938102375
short report: verruga peruana in an italian traveler from peru.we report a case of cutaneous bartonellosis in a 24-year-old italian woman. soon after returning from a seven-month stay in a central area of peru at an altitude of 3,130 meters, she developed high fever, headache, and muscle and joint pain that resolved after antibiotic therapy. two months later, she presented with disseminated, painless, firm, purple, subcutaneous nodules on the neck, shoulders, legs, left eyelid, and hard palate, some of which developed into vegetating lesions. the diagnosis ...19948116804
taxonomic position of the rickettsiae: current knowledge.the term rickettsiae initially encompassed all intracellular bacteria. early rickettsial taxonomy was based on a comparison of a few phenotypic characteristics and recently, molecular studies brought new bases for rickettsial taxonomy. all rickettsial species studied so far belong to the alpha and gamma groups of the proteobacteria. ehrlichiae complex groups cowdria ruminantium, anaplasma marginale and wolbachia pipientis and the related parthenogenesis and cytoplasmic incompatibility bacteria, ...19948117465
bacillary angiomatosis. clinical and histologic features, diagnosis, and treatment.bacillary angiomatosis is a relatively new infection affecting primarily patients with human immunodeficiency virus or others with impaired host defenses. it presents most commonly with multiple red skin lesions, but visceral involvement may also occur, including involvement of the liver and spleen. because of the dermatologic manifestations, bacillary angiomatosis may be mistaken for kaposi's sarcoma. the diagnosis is made by identification of the characteristic histologic findings or genetic a ...19948122945
diagnostic tests for rocky mountain spotted fever and other rickettsial diseases.rickettsial diseases continue to confound physicians because of their usually sporadic occurrence and nonspecific clinical presentation. in past years, diagnosis was confirmed only during convalescence; however, newer, more widely available methods, such as antigen detection and improved in vitro cultivation, have enabled a diagnosis at a time when therapeutic decisions are made. molecular nucleic acid-based techniques have also expanded the spectrum of etiologic agents and illnesses caused by r ...19948143383
rochalimaea henselae infections: newly recognized zoonoses transmitted by domestic cats. 19948144389
rochalimaea henselae organisms possess an elevated capacity of binding to peripheral blood lymphocytes from patients with cat scratch disease.cat scratch disease (csd) is a clinical condition whose aetiological agent, according to recent findings, is of bacterial origin. two gram-negative bacteria are invoked as causative agents of csd, namely afipia felis and rochalimaea henselae. in this paper, five patients with suspected csd were studied in terms of binding capacity of a. felis and r. henselae to their own peripheral blood lymphocytes (pbl). this parameter was correlated with serum antibody titres to both a. felis and r. henselae, ...19948152395
atypical cat-scratch disease: diagnosis by a serologic test for rochalimaea species.three patients with atypical manifestations of cat-scratch disease (csd) had the diagnosis supported by a newly available serologic assay for antibodies to rochalimaea henselae. previously, csd has been a diagnosis of exclusion because no confirmatory test was readily available. atypical or severe cases of csd have often required detailed (and expensive) clinical and laboratory investigation. the use of rochalimaea-specific serologic tests may help avoid extensive diagnostic testing or invasive ...19948153776
cat scratch disease, bacillary angiomatosis, and other infections due to rochalimaea. 19948164704
bacillary angiomatosis of the spleen.bacillary angiomatosis is a recently described vasoproliferative lesion associated with infection by a newly characterized rickettsial organism, rochalimaea henselae. most previous reports have described skin lesions in immunocompromised patients infected with human immunodeficiency virus. this is the first case report detailing the features of bacillary angiomatosis of the spleen occurring in a patient undergoing cytotoxic chemotherapy for disseminated ovarian carcinoma.19938165007
morphologically variable bacilli of cat scratch disease are identified by immunocytochemical labeling with antibodies to rochalimaea henselae.the identification of the causative organisms of cat scratch disease (csd) has been elusive. the demonstration of warthin-starry stain-positive pleomorphic bacilli in lymph nodes of patients with csd and recent serologic and epidemiologic data suggest an etiologic role of rochalimaea henselae in csd. the authors studied lymph node biopsy specimens of 46 patients with illnesses clinically consistent with csd and found pleomorphic bacilli in 15 (33%). the organisms were labeled by polyclonal rabbi ...19948178768
identification of outer membrane proteins of bartonella bacilliformis.purification of the outer membrane of bartonella bacilliformis by sucrose step gradient centrifugation and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) suggest that 14 proteins, ranging from 11.2 to 75.3 kda, are located in the outer membrane of the pathogen. on the basis of m(r)s, eleven of these proteins have counterparts which are labeled by extrinsic radioiodination of intact bartonellae, and two of the proteins are visibly sensitive to extrinsic proteinas ...19948188391
characterization of bartonella bacilliformis flagella and effect of antiflagellin antibodies on invasion of human erythrocytes.bartonella bacilliformis is the etiologic agent of oroya fever in humans. flagellum-mediated motility has been postulated as a major virulence factor for invasion of host cells. to address this hypothesis, we purified and characterized flagella from strain kc584 and then assessed their role in human erythrocyte association and invasion. electron microscopy of the flagellar preparation showed a high concentration of filaments with a mean wavelength of 800 nm. sodium dodecyl sulfate-polyacrylamide ...19938225570
proposals to unify the genera bartonella and rochalimaea, with descriptions of bartonella quintana comb. nov., bartonella vinsonii comb. nov., bartonella henselae comb. nov., and bartonella elizabethae comb. nov., and to remove the family bartonellaceae from the order rickettsiales.dna hybridization data (hydroxyapatite method, 50 to 70 degrees c) indicate that rickettsia prowazekii, the type species of the type genus of the family rickettsiaceae, is substantially less closely related to rochalimaea species than was previously thought. the levels of relatedness of rickettsia prowazekii to rochalimaea species and to bartonella bacilliformis under optimal conditions for dna reassociation were 0 to 14%, with 25.5% or greater divergence in related sequences. when stringent rea ...19938240958
ehrlichia chaffeensis and rochalimaea antibodies in kawasaki disease.sera from 38 patients with kawasaki disease were tested for immunofluorescent antibodies to ehrlichia chaffeensis, rochalimaea henselae, and r. quintana oklahoma. only 2.5% of the patients tested positive for e. chaffeensis, and 5% were positive for r. henselae and r. quintana oklahoma. our data suggest that ehrlichia and rochalimaea spp. do not play a unique role in the etiology of kawasaki disease.19938263201
cat-scratch disease mimicking rhabdomyosarcoma.a 12-year-old boy who had injured his elbow presented with a lump in the epitrochlear area. originally thought to be a hematoma, then later myositis ossificans, this mass was actually a lymph node with large, reactive germinal centers. although stains for acid-fast bacilli, fungal organisms, bacteria, and cat-scratch disease were negative, serological testing for detection of rochalimaea henselae organisms found evidence of cat-scratch disease. we would like to remind orthopaedic surgeons to con ...19938265222
[bacillary angiomatosis of the oral cavity in aids. a differential diagnosis of mucosal kaposi's sarcoma].the diagnosis of bacillary angiomatosis (ba) was performed on a biopsy of a reddish nodular lesion of the gingival mucosa, in a patient seropositive for the h.i.v. it was based on the histopathological features, and by the demonstration of bacilli both on the warthin-starry stain and the electron microscopic study. a treatment by erythromycin was quickly effective. ba is an opportunistic infection seen essentially in patients with a.i.d.s., most often causing cutaneous involvement. the causative ...19938278795
hepatosplenic cat-scratch disease associated with elevated anti-rochalimaea antibody titers. 19938284124
antimicrobial susceptibility of rochalimaea quintana, rochalimaea vinsonii, and the newly recognized rochalimaea henselae.the susceptibility of an isolate of rochalimaea quintana, rochalimaea vinsonii and the newly recognized rochalimaea henselae to antimicrobial agents was determined by dilution in mueller-hinton agar supplemented with 5% sheep blood. two inocula of 10(2) and 10(4) cfu were used and tests were read after incubation at 5 and 7 days at 37 degrees c in an atmosphere enriched to 5% carbon dioxide. each isolate was susceptible to amoxycillin, third-generation cephalosporins, tetracyclines, macrolides a ...19938288500
rochalimaea henselae infection. a new zoonosis with the domestic cat as reservoir.to determine the reservoir and vector(s) for rochalimaea henselae, a causative agent of bacillary angiomatosis (ba) and cat scratch disease, and to estimate the percentage of domestic cats with r henselae bacteremia in the greater san francisco bay region of northern california.19948301768
rochalimaea infections. are they zoonoses? 19948301773
[bacillary peliosis in aids. anatomo-clinical study of 2 cases].the authors report two cases of peliosis hepatis, occurring in patients with aids, who presented a persistent fever and an hepatomegaly. the liver biopsies showed areas of peliosis, where bacilli were observed by warthin-starry stain. in one case, techniques of molecular biology allowed the identification of rochalimaea henselae, pathogen involved in bacillary angiomatosis. this rickettsia has been newly recognized in the united-states, where 17 cases of bacillary peliosis have been published in ...19938311862
disseminated cat-scratch disease: detection of rochalimaea henselae in affected tissue.an immunocompetent 9-year-old boy with disseminated cat scratch disease involving spleen, cervical and abdominal lymph nodes, skull, and one clavicle is reported. antibodies to rochalimaea quintana and r. henselae were detected, at increasing, then decreasing concentration. dna extracted from the biopsied skull lesion was amplified by polymerase chain reaction and hybridized with species-specific oligonucleotides proving the presence of r. henselae in affected tissue. our findings suggest that r ...19948313920
[bacillary angiomatosis. a pseudoneoplastic infection in aids patients].bacillary angiomatosis is a newly recognized pseudoneoplastic vascular disease seen in the setting of human immunodeficiency virus (hiv) infection. the disease is characterized by a cutaneous infection with reddish papules or nodules that are similar to pyogenic granulomas or kaposi's sarcoma in clinical appearance. it is caused by the mildly gram-negative bacillus rochalimaea henselae, which can be identified in tissue sections by means of warthin-starry stain. the historical, clinical, histopa ...19938335458
cat scratch disease and rochalimaea henselae. 19938336627
development and evaluation of a blood-free medium for determining growth curves and optimizing growth of rochalimaea henselae.two strains of rochalimaea henselae were used to optimize a blood-free growth medium. seven agar bases, four broths, and combinations of eight supplements were evaluated. acceptable growth was achieved in media containing fildes solution and hemin, with the best growth demonstrated in brucella broth or on brucella agar with 6 to 8% fildes solution and 250 micrograms of hemin per ml. r. henselae utilized hemin in concentrations six times that utilized by rochalimaea quintana. erythrocyte membrane ...19938349767
animal-associated opportunistic infections among persons infected with the human immunodeficiency virus.a number of animal-associated infections occur in persons infected with the human immunodeficiency virus (hiv), including those due to toxoplasma gondii, cryptosporidium, microsporida, salmonella, campylo-bacter, giardia, rhodococcus equi, rochalimaea, and listeria monocytogenes. most of these infections, with the exception of those due to rochalimaea, appear to be acquired by the immunosuppressed individual from sources other than exposure to animals. drs. glaser and colleagues review our curre ...19948054433
detection of rochalimaea henselae in cat-scratch disease skin test antigens.one of the diagnostic criteria for cat-scratch disease is a positive delayed-type hypersensitivity skin test reaction to an antigen prepared from purulent material aspirated from a cat-scratch disease-involved lymph node. polymerase chain reaction coupled with dna sequence analysis was used to identify organisms found in these skin test antigen preparations that may be responsible for eliciting this response. two independent sources of cat-scratch disease skin test antigens yielded dna sequences ...19938376816
association between rochalimaea infection and cat-scratch disease. 19938399887
endocarditis caused by rochalimaea henselae.a case of community-acquired, culture-negative, infective endocarditis was diagnosed in a 57-year-old construction worker. small, pleomorphic gram-negative rods were seen in brown-hopps tissue gram stains and warthin-starry silver stains. the organism was identified as rochalimaea henselae by polymerase chain reaction amplification and sequencing of the 16s rdna gene sequence. this is the first report of infective endocarditis caused by r henselae.19938406424
epidemiologic observations on infection with rochalimaea species among cats living in baltimore, md.cats from several sources in baltimore, md, were tested for seropositivity to rochalimaea henselae and r quintana. co-infection with toxoplasma gondii or feline immunodeficiency virus was assessed as a risk factor for infection with rochalimaea spp. of 592 cats tested, 87 (14.7%) were seropositive for one or both rochalimaea spp, although titers to r henselae were significantly higher than those to r quintana. prevalence of seropositivity increased significantly with cat age and weight and was a ...19948063598
the epidemiology of bacillary angiomatosis and bacillary peliosis.to determine environmental risk factors for bacillary angiomatosis-bacillary peliosis (bap), and to confirm infection with rochalimaea species.19938423659
bacillary angiomatosis: a new entity in acquired immunodeficiency syndrome.since the recognition of the acquired immunodeficiency syndrome (aids) in 1981, previously rare infections and neoplasms have become increasingly common. bacillary angiomatosis, undescribed in the medical literature prior to 1983, is now second in frequency only to kaposi's sarcoma with respect to the cutaneous manifestations associated with human immunodeficiency virus (hiv) infection. caused by rochalimaea henselae, bacillary angiomatosis is easily treated, when diagnosed early, with erythromy ...19948064965
bacteremia due to rochalimaea henselae in a child: practical identification of isolates in the clinical laboratory.two closely related species of rochalimaea, rochalimaea quintana and rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. we report a case of culture-proven r. henselae bacteremia in a child with persistent fever. serologic evidence of infection by r. henselae was ascertained by testing sera at two intervals for immunoglobulin g or immunoglobulin m antibodies by enzyme immunoassay and immun ...19938408560
eia for detection of rochalimaea henselae-reactive igg, igm, and iga antibodies in patients with suspected cat-scratch disease. 19938501350
cat scratch disease in connecticut. epidemiology, risk factors, and evaluation of a new diagnostic test.although cat scratch disease is commonly diagnosed in patients who have unexplained regional lymphadenopathy after encounters with cats, its epidemiology and the risk factors for disease are not clearly defined, and there is no generally accepted diagnostic test.19938505963
[rochalimaea henselae infection. febrile pancytopenia and hepatic peliosis in a patient with hiv infection].a case of rochalimaea henselae infection in an aids patient is reported. the r. henselae infection was revealed by febrile pancytopenia associated with liver and spleen enlargement. the diagnosis was made on the finding at histology of hepatic peliosis lesions. within these lesions warthin starry staining displayed bacilli that were identified as r. henselae. this case shows that all hiv-infected patients with these symptoms might be infected with this organism.19938511079
physical map of the bartonella bacilliformis genome.the genome of bartonella bacilliformis was shown to be a single circular dna molecule of about 1,600 kbp having six noti, four sfii, and two ceui sites. a physical map of the dna was constructed by contour-clamped homogeneous electric field pulsed-field gel electrophoresis of dna restriction fragments. rrna operons, the invasion-associated locus, and a flagellin gene were located on the map by hybridization.19958522538
fever, abdominal pain and an intracranial mass. 19958532442
lack of bactericidal effect of antibiotics except aminoglycosides on bartonella (rochalimaea) henselae.bartonella (rochalimaea) henselae is a cause of peliosis hepatis and bacillary angiomatosis, and one of the putative agents of cat scratch disease. specific therapy for b. henselae infections is not available. treatment failures and relapses are frequent, especially following brief antibiotic courses, and this contrasts with the in-vitro susceptibility of b. henselae to most antibiotics. we decided to test the antibiotic susceptibility of b. henselae associated with murine macrophage-like cells ...19958537257
[encephalopathy in cat scratch disease]. 19958545389
rochalimaea infections. 19938357124
syndrome of rochalimaea henselae adenitis suggesting cat scratch disease.to describe a clinical syndrome of cat scratch disease caused by rochalimaea henselae, including methods for isolation of the organism from tissue and for identification.19938430978
[bacillary angiomatosis, cat-scratch disease and trench fever: are these diseases caused by the same bacteria?]. 19968553491
imaging manifestations of cat-scratch disease.cat-scratch disease affects an estimated 22,000 people in the united states each year, more than half of whom are children or adolescents [1]. it is caused by bartonella henselae, a gram-negative bacillus usually introduced by the scratch of a cat [2]. in the past, diagnosis was made if three of the following four criteria were met: (1) history of cat exposure with inoculation, (2) positive skin test: (3) absent laboratory and histopathologic evidence of other diseases, and (4) biopsy findings o ...19968553962
cat-scratch disease: single or multiple causative microorganisms? 19958559644
neuroretinitis, aseptic meningitis, and lymphadenitis associated with bartonella (rochalimaea) henselae infection in immunocompetent patients and patients infected with human immunodeficiency virus type 1.bartonella (rochalimaea) henselae causes a variety of diseases, including bacillary angiomatosis, peliosis hepatis, lymphadenitis, aseptic meningitis with bacteremia, and cat-scratch disease (csd). cases of b. henselae-related disease were collected from september 1991 through november 1993. patients with suspected csd, unexplained fever and lymphadenitis, or suspected b. henselae infection who were seen in the infectious diseases clinic at wilford hall medical center (lackland air force base, t ...19958562744
endocarditis caused by rochalimaea quintana in a patient infected with human immunodeficiency virus.rochalimaea quintana and rochalimaea henselae are closely related, fastidious, gram-negative rickettsiae. thus far, the spectrum of human rochalimaea sp. infections has not included endocarditis. we describe a 50-year-old human immunodeficiency virus-positive man who developed endocarditis caused by r. quintana. dna relatedness studies, which compared our patient's blood culture isolate with known rochalimaea species, identified the organism as r. quintana. our report expands the spectrum of roc ...19938458964
verruga peruana: an infectious endemic angiomatosis.microbial-related dysplastic and neoplastic angiomatous proliferative processes are seen with increased frequency, particularly in the acquired immunodeficiency syndrome (aids). the microbial-encoded or -induced mediators of angiopathogenesis in aids-associated kaposi's sarcoma and bacillary angiomatosis are actively being sought. the present review addresses the historical, epidemiologic, clinical, etio- and histopathogenic aspects of the verruga peruana (vp). vp is a disease thus far endemic t ...19958573607
intracellular location of bartonella henselae cocultivated with vero cells and used for an indirect fluorescent-antibody test.bartonella henselae, the major causative agent of cat scratch disease, was cocultivated with vero cells on chamber slides and visualized by indirect immunofluorescence by using a patient serum containing specific antibodies. confocal microscopy localized the granular b. henselae-specific fluorescence mainly around the nuclei of vero cells. by transmission electron microscopy, these granules were identified as clusters of multiple intracellular organisms. fixed slides with the monolayers of vero ...19958574831
cat-scratch disease simulating lymphoma. 19968576472
serology to bartonella (rochalimaea) henselae may replace traditional diagnostic criteria for cat-scratch disease.bartonella (rochalimaea) henselae has been identified as causative agent of cat-scratch disease (csd). employing an indirect fluorescence antibody test we found that 20 (100%) out of 20 children with suspected csd had serum titres to b. henselae of > or = 512. by contrast, in all but one of the controls (n = 332) including subjects exposed to the same cats, patients with diseases other than csd, and blood donors the antibody titres were < or = 256 (p < 0.001). however, significant regional diffe ...19958582404
[cat scratch disease caused by bartonella henselae].bartonella henselae is an etiologic agent of cat-scratch disease and, in immunocompromised patients, of bacillary angiomatosis and other severe syndromes. cat-scratch disease usually presents as lymphadenopathy, which resolves spontaneously within 2-4 months. the utility of antibiotic therapy remains controversial. in tyrol four cases of human cat-scratch disease were diagnosed in children in 1994, yielding a prevalence of 0.7/100,000 per year. a 3-year-old boy had lymphadenitis coli since one y ...19958582740
cluster of five children with acute encephalopathy associated with cat-scratch disease in south florida.between august 12 and september 27, 1994, five children in south florida were hospitalized at a single hospital because of encephalopathy, presenting as status epilepticus, associated with cat-scratch disease (csd). diagnoses were confirmed by using an indirect fluorescent antibody test to detect antibody to bartonella henselae, the causative agent of csd. these cases represent the first cluster of csd encephalopathy cases to be recognized in the united states. the patients lived within 7 miles ...19958584313
sudden onset of blurred vision in a thirteen-year-old girl. 19958584347
mics of 28 antibiotic compounds for 14 bartonella (formerly rochalimaea) isolates.we assessed in vitro the antibiotic susceptibilities of 14 bartonella isolates of the species b. quintana, b. vinsonii, b. henselae, and b. elizabethae. columbia agar base supplemented with 5% horse blood was used as the antibiotic assay medium. bacterial growth could be evaluated within 5 days after incubation of the plates at 37 degrees c in a 5% carbon dioxide atmosphere. the mics at which 90% of isolates are inhibited (mic90s) were 0.06 microgram/ml for penicillin g and amoxicillin and 0.25 ...19958585713
prolonged bartonella bacteremia in cats associated with cat-scratch disease patients.recent evidence supports a causal relationship between bartonella (rochalimaea) henselae, cat-scratch disease (csd), and bacillary angiomatosis. cats appear to be the primary reservoir. blood from 19 cats owned by 14 patients diagnosed with csd was cultured. blood samples from cats owned by veterinary students (n = 25) having no association with csd or bacillary angiomatosis were cultured as controls. eighty-nine percent (17 of 19) of cats associated with csd patients and 28% (7 of 25) of contro ...19958586710
inter- and intraspecies identification of bartonella species by pcr-restriction fragment length polymorphism analysis of a fragment of the ribosomal operon. 19958586746
bartonella henselae is a causative agent of cat scratch disease in australia.we report the first isolation of bartonella henselae from the blood and fleas of a cat of a patient with cat scratch disease (csd) in australia. a 49-year-old man presented with a history that 3 weeks after he had removed fleas from his cat he had developed fever, lethargy and anorexia for 3 days. this was followed by the appearance of axillary lymphadenopathy. there was no history of a bite or scratch and no primary lesion on the skin. two fine needle aspirates of the axillary lymph node showed ...19958586847
cat-scratch disease diagnosed serologically using an enzyme immunoassay in a patient with neuroretinitis. 19968602794
seroprevalence to bartonella quintana among patients at a community clinic in downtown seattle.in 1993, an outbreak of 10 cases of bartonella quintana bacteremia occurred among homeless, alcoholic, human immunodeficiency virus (hiv)-negative persons in seattle. to estimate the prevalence of past exposure b. quintana among this population, a serosurvey was conducted in 1994 among patients at a downtown seattle clinic. microimmunofluorescent titers to b. quintana in 192 clinic patients were compared with titers in 199 age- and sex-matched seattle volunteer blood donors. titers > or = 64 wer ...19968603944
mr imaging of the knee: findings in cat-scratch disease. 19968615286
carboxyl-terminal processing protease for the d1 precursor protein: cloning and sequencing of the spinach cdna.a previous study has demonstrated that the carboxyl-terminal (c-terminal) processing protease in spinach for the d1 precursor protein (pdl) of the photosystem ii reaction center is a monomeric protein of about 45 kda. based on the amino acid sequence data of the purified protease, a cdna clone encoding the enzyme has been identified and sequenced, from a spinach green leaf cdna library. in order to determine the 5' end of the transcript, the rapid amplification of cdna end (5'-race) technique wa ...19968616242
new serotype of bartonella henselae in endocarditis and cat-scratch disease.the fastidious nature of bartonella henselae is such that demonstration of clinical infection relies mainly on serological or molecular biological methods. only five isolates have been obtained from patients with cat-scratch disease (csd) and none from endocarditis.19968618486
[cat-scratch disease and other infections caused by bartonella species].bartonella henselae, the causative agent of cat-scratch disease, was identified recently by dna amplification techniques. several other bartonellae (most of which were called rochalimaea before) cause disease in humans: b. bacilliformis (carrion's disease), b. elizabethae (endocarditis) and b. quintana (bacillary angiomatosis and peliosis, chronic bacteraemia and endocarditis, trench fever). b. henselae is transmitted to humans by scratch or bite of a bacteraemic, but asymptomatic, cat, which ev ...19968618634
etiology of cat-scratch disease. 19968623713
bacillary angiomatosis in hiv-infected patients: report of three cases with different clinical courses and identification of rochalimaea quintana as the aetiological agent.three cases of cutaneous bacillary angiomatosis in hiv-infected patients are reported. they differed profoundly with respect to the extent of the lesions and the clinical course. in two cases, rochalimaea quintana was identified by direct sequencing of the dna amplified with the polymerase chain reaction (pcr), whereas an easy, rapid method based on the restriction length of polymorphism analysis of pcr products (pcr-rflp) was used in the third case. this report illustrates the variations in cli ...19958547056
[cat scratch disease. bartonella henselae antibodies and dna detection in regional lymphadenopathy].history and clinical findings: two hard, pressure-sensitive nodules developed in the lower jaw of a 22-year-old woman. after a dental cause had been excluded, she was treated for suspected tonsillitis with ceftibuten. erythrocyte sedimentation rate was increased to 18 mm in the first hour. there were no other significant biochemical findings and fine-needle biopsy of one of the nodules showed nonspecific inflammatory reaction. investigations: sonography revealed two lymph nodes, 7 and 22 mm in d ...19968631227
isolation of bartonella henselae from domestic cats in japan.during the period from january to march 1995, the authors first isolated bartonella henselae from the blood of three (9.1%) of 33 domestic cats in japan. the three cats were a 1.5-year male pet cat-old with urinary retention, and 6-year-old female pound and age-unknown female pet cats with no abnormalities. the blood was taken in a lysis-centrifugation tube (wampole isolator tube) and cultured on 5% rabbit-blood heart infusion agar plates at 35 degrees c in the 5% co2 atmosphere. visible tiny ro ...19968645765
bartonella henselae endocarditis in an immunocompetent adult.we describe a case of aggressive bartonella henselae endocarditis in an immunocompetent man who owned a cat. aortic valve replacement was required, and his infection was diagnosed by histology, serology, and polymerase chain reaction analysis. the manifestations of his disease included mediastinal lymphadenopathy, glomerulonephritis, myocarditis, and a petechial rash; the unusual finding of a positive titer of c-antineutrophil cytoplasmic antibodies was noted. serological titers were markedly el ...19958645787
cat-scratch disease, bartonella henselae, and the usefulness of routine serological testing for afipia felis. 19958645822
use of the polymerase chain reaction and dna sequencing for detection of bartonella quintana in the aortic valve of a patient with culture-negative infective endocarditis.we used the polymerase chain reaction (pcr) and broad-range bacterial primers, combined with dna sequencing, to identify bartonella quintana as the etiologic agent in a case of culture-negative infective endocarditis; all blood cultures, as well as the bacterial cultures of the resected aortic valve and vegetations, remained negative. pcr was used to amplify bacterial 16s rdna from a template prepared from the aortic valve vegetation. the amplified 16s rdna produced a nucleotide sequence that wa ...19958645836
bartonella (rochalimaea) antibodies, dementia, and cat ownership among men infected with human immunodeficiency virus.to determine the association between recent human immunodeficiency virus (hiv)-associated dementia and serum antibodies to bartonella (rochalimaea) henselae, we performed a nested case control study within the multicenter aids cohort study in los angeles. we measured serum igg and igm antibodies to b. henselae with use of enzyme immunoassay in 369 hiv-seropositive and hiv-seronegative participants with and without recent neuropsychological deterioration. data on pet ownership were also collected ...19958645846
acquisition of the cat scratch disease agent bartonella henselae by cat fleas (siphonaptera:pulicidae).we assayed the ability of cat fleas to become infected with bartonella henselae, using an artificial feeding device. fleas fed a concentration of 1 x 10(5) cfu/ml in blood were examined using immunofluorescent antibody assay and polymerase chain reaction. bacteria were present in the gut at 3 h, and persisted up to 9 d after infection. qualitatively, the density of b. henselae was greater in the flea gut at 9 d, indicating that replication was occurring in the gut. b. henselae also was detected ...19968667399
unknown species of rickettsiae isolated from ixodes ricinus tick in wałcz.ticks ixodes ricinus have been harvested in park down-town wałcz in june 1994. presence of borrelia burgdorferi and rickettsiae has been tested with pcr. b. burgdorferi and coxiella burnetii have not been detected in any tick whereas characteristic for other rickettsiae dna fragment of gene encoding citrate-synthase has been found. bacterial strain has been cultured from the tick. biochemical properties of isolated strain has strongly suggested that these bacteria belong to genus bartonella (roc ...19968673798
[cat scratch disease. atypical forms].first described by robert debré in 1950, cat scratch disease, usually observed in children and young adults (80% of the case occur in subjects under 18), is the principal cause of chronic benign lymph node enlargement. the centers for disease control at atlanta currently recognize afipia felis and rochalimaea henselae as the causal agents of cat scratch disease. cats transmit the disease to humans by skin scratches or by licking open wounds. the bacilli can also be transmitted by dogs, monkeys, ...19968685112
bilateral optic disc edema and multifocal retinal lesions without loss of vision in cat scratch disease. 19968694710
widespread cutaneous bacillary angiomatosis and a large fungating mass in an hiv-positive man.bacillary angiomatosis (ba), an infection caused by a gram-negative rod, can be a multiorgan disease. the usual causative organism, bartonella (formerly rochalimaea) hensalae, has only recently been identified. bartonella quintana has also been shown to cause some cases of cutaneous ba. we describe a patient with widespread cutaneous ba with probable bone involvement and a large fungating mass.19968698906
bacillary angiomatosis: microbiology, histopathology, clinical presentation, diagnosis and management.bacillary angiomatosis is known to be caused by a rickettsial organism; rochalimaea henselae. this causative agent has been compared with different microorganisms and clinical conditions that appear in similar settings buy have been clearly differentiated from them; e.i. cat-scratch disease (afipia felis), bartonella bacilliformis, other rochalimaea sp., kaposi's sarcoma, lobular capillary hemangioma, angiosarcoma, and epithelioid hemangioma. clinically the bacillary angiomatosis (ba) skin lesio ...19958703269
new pathogens, and diseases old and new. i) afipia felis and rochalimaea. ii) parvovirus b 19. iii) herpesvirus 6.the paper describes events that in the last fifteen years, have led to the identification of the aetiological agents of three widely known diseases: cat scratch disease, erythema infectiosum and exanthem subitum. the particular features of afipia felis and rochalimaea, parvovirus b 19 and herpesvirus 6 are presented. the paternity of new diseases (i.e. bacillary angiomatosis, bacillary peliosis hepatitis, les-like syndrome, chronic fatigue syndrome, petechial glove and sock syndrome, etc.) has a ...19958710408
bartonella (rochalimaea) infections: beyond cat scratch.five species of bartonella have been found to infect humans, henselae, quintana, elizabethae, bacilliformis, and vinsonii. the most common of these in north america are bartonella quintana and bartonella henselae, the agents of trench fever, bacillary angiomatosis, and parenchymal peliosis, and in the case of b. henselae cat-scratch disease. b. bacilliformis is endemic in peru and ecuador, where it causes oroya fever or carrion's disease. new methods of diagnosing bartonella infections have resu ...19968712787
bartonella (rochalimaea) quintana causing fever and bacteremia in an immunocompromised patient with non-hodgkin's lymphoma.a 48-yr-old man with stage iv non-hodgkin's lymphoma, became neutropenic following chemotherapy and developed a fever. his blood cultures were processed to enhance the yield of fastidious bacteria. a slow-growing, capnophilic gram-negative rod was isolated. the febrile episode was treated with cefotaxime, imipenem and vancomycin and resolved. the bacterial isolate was identified as bartonella (rochalimaea) quintana by 16s-rdna gene sequencing. the isolate showed 99.8% sequence homology with the ...19968714279
bartonella infections. 19968718458
[detection of bartonella (rochalimaea) henselae/b. quintana by polymerase chain reaction (pcr)].bartonella (rochalimaea) henselae and/or b. quintana are the causative agents of a variety of infections such as trench fever, bacillary angiomatosis, septicemia, peliosis hepatis and endocarditis. recently, b. henselae has been identified as a major cause of cat scratch disease. diagnosis of such infections is based on clinical information, histopathology, culture and serology. however, none of these methods alone is sufficiently sensitive or specific. we have used the pcr to search for dna spe ...19968720724
q fever and trench fever. 19968727131
bacillary angiomatosis. 19968727133
bartonella (rochalimaea) quintana infection in a seronegative hemodialyzed patient.bartonella quintana is a reemerging pathogen responsible for trench fever, endocarditis, bacteremia, and bacillary angiomatosis. we previously reported the first case of a patient with b. quintana-induced chronic adenomegaly, and here we present a report on a second patient. a hemodialyzed patient with sjögren's syndrome presented with mediastinal adenomegalies and secondary pancytopenia. all diagnostic investigations remained negative, except that a bartonella-like microorganism was isolated fr ...19968727894
bartonella (rochalimaea) henselae hepatosplenic infection occurring simultaneously in two siblings. 19968729220
[visceral localizations of cat-scratch disease in an immunocompetent patient].locoregional expression of cat scratch disease is well known, but despite advances in microbiology over the last 10 years leading to the description of two new bacteria (afipia felis, bartonella henselae) the infective agent responsible for cat scratch syndrome remains unknown. until the 80s, only one systemic disease was attributed to infection with a germ in the bartonella genus: trench fever. with the onset of the aids epidemic, new clinical syndromes caused by bartonella bacteria have been d ...19968729380
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