| isolation and characterization by immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot, restriction fragment length polymorphism-pcr, 16s rrna gene sequencing, and pulsed-field gel electrophoresis of rochalimaea quintana from a patient with bacillary angiomatosis. | rochalimaea quintana was isolated from the blood of a french human immunodeficiency virus-infected patient with bacillary angiomatosis. the isolate showed the typical growth characteristics of rochalimaea species and was inert when typical biochemical testing was used. the purpose of the present work was to characterize and compare this new isolate with reference strains of r. quintana, rochalimaea vinsonii, and rochalimaea henselae by using immunofluorescence, sodium dodecyl sulfate-polyacrylam ... | 1994 | 7519628 |
| detection of bartonella (rochalimaea) quintana by routine acridine orange staining of broth blood cultures. | bartonella quintana was isolated from 34 bactec nonradiometric aerobic resin blood cultures for 10 adults. nine patients were initially diagnosed by routine acridine orange staining of routine cultures that had been incubated for 8 days. all subcultures grew on chocolate agar within 3 to 12 days (median, 6 days). the plus 26 high-volume aerobic resin medium, combined with acridine orange stain and subculture, is an effective system for detection and isolation of b. quintana from blood. | 1994 | 7521357 |
| bacillary angiomatosis: investigation of the unusual interactions between rochalimaea bacilli and endothelial cells. | | 1994 | 7523552 |
| rochalimaea species stimulate human endothelial cell proliferation and migration in vitro. | rochalimaea henselae and r. quintana are associated clinically with proliferative neovascular lesions. the effect of rochalimaea species on human umbilical vein endothelial cell (huvec) proliferation and migration was evaluated in vitro. cocultivation of rochalimaea organisms with huvecs resulted in enhanced huvec proliferation. fibroblast proliferation was unaffected by r. henselae. huvecs were also stimulated to migrate by rochalimaea. when r. henselae organisms were disrupted and subjected to ... | 1994 | 7523553 |
| [a new bacterium: rochalimaea]. | originally limited to trench fever, infections due to rochalimaea now comprise manifestations particular to patients with human immunodeficiency virus (bacillary angiomatosis and hepatic peliosis), but also manifestations as diverse as isolated fever, septicaemia, endocarditis, lymphocytic meningitis, or central neurological disorders, in immunodepressed or immunocompetent subjects. the involvement of rochalimaea in cat-scratch fever remains debated. microbiological analysis used for diagnosis h ... | 1994 | 7524133 |
| [rochalimaea henselae, afipia felis and cat-scratch disease]. | several years ago, rochalimaea henselae has emerged as an agent of bacillary angiomatosis, bacillary peliosis and recurrent septicaemia that generally occur in patients infected with human immunodeficiency virus. an aetiologic role in cat scratch disease is also suspected widely on the basis of a serologic survey. its slow growth and its culture requirement explain that this pathogen, a gram-negative bacterium, could not be isolated until 1990. moreover, blood and tissue samples request lysis an ... | 1994 | 7524253 |
| cat-scratch disease in italy: a serological approach. | the results of studies on the serologic responses to afipia felis and rochalimaea henselae in suspected patients for cat scratch disease (csd) are illustrated. this preliminary study performed using indirect immunofluorescence assay, proved negative for a. felis and r. henselae in some patients and positive in others; in a few instances the test was positive for both organisms. additional microbiological and serological studies are needed to clarify the exact role of these microorganisms in caus ... | 1994 | 7526131 |
| fever, back pain and pleural effusion in a four-year-old boy. | | 1994 | 7526327 |
| bacteriophage-like particle of rochalimaea henselae. | an extracellular particle approximately 40 nm in diameter was detected in culture supernatant from the fastidious bacterium rochalimaea henselae. this particle has at least three associated proteins and contains 14 kbp linear dna segments that are heterogeneous in sequence. the 14 kbp dna was also present in r. henselae cells as an extrachromosomal element for all 14 strains tested. despite attempts to induce lysis of r. henselae, plaque formation was not observed. a similar particle, also conta ... | 1994 | 7527118 |
| encephalitis associated with cat scratch disease--broward and palm beach counties, florida, 1994. | on august 14, 1994, the broward county public health unit of the florida department of health and rehabilitative services was notified of three children from pompano beach who were hospitalized with encephalitis attributed to cat scratch disease (csd). all three children (aged 5, 6, and 11 years) were previously healthy and had no histories of seizure disorders or diagnoses of csd. this report summarizes the investigation of these cases. | 1994 | 7527121 |
| cat scratch disease. | | 1994 | 7527210 |
| positive predictive value of rochalimaea henselae antibodies in the diagnosis of cat-scratch disease. | | 1994 | 7527255 |
| downregulation of human polymorphonuclear cell activities exerted by microorganisms belonging to the alpha-2 subgroup of proteobacteria (afipia felis and rochalimaea henselae). | intracellular pathogens have evolved effective mechanisms in order to survive in an intracellular environment, thus avoiding destruction by phagocytic cells. in this regard, a correlation between resistance to phagocytic killing and expression of pathogenic potency has been established. in this report, we have studied the interaction between human polymorphonuclear cells (pmn) and two gram-negative microorganisms, afipia felis and rochalimaea henselae, which belong to the alpha-2 subgroup of the ... | 1994 | 7528238 |
| cat scratch disease. | | 1994 | 7528453 |
| persistent infection with bartonella (rochalimaea) henselae or afipia felis is unlikely to be a cause of chronic fatigue syndrome. | | 1994 | 7528553 |
| neuroretinitis in cat-scratch disease associated with the macular star. | | 1994 | 7528909 |
| rochalimaea--a cosmopolitan zoonosis? | | 1994 | 7529453 |
| bartonella (rochalimaea) quintana endocarditis in three homeless men. | bartonella (rochalimaea) quintana is the agent of trench fever and is transmitted by the body louse. we searched for this organism in three alcoholic homeless men with endocarditis. | 1995 | 7529894 |
| bartonella (rochalimaea) quintana bacteremia in inner-city patients with chronic alcoholism. | bartonella (rochalimaea) quintana is a fastidious gram-negative bacterium known to cause trench fever, cutaneous bacillary angiomatosis, and endocarditis. between january and june 1993 in seattle, we isolated b. quintana from 34 blood cultures obtained from 10 patients not known to be infected with the human immunodeficiency virus (hiv). | 1995 | 7529895 |
| has trench fever returned? | | 1995 | 7529896 |
| cat-scratch encephalopathy: a cause of status epilepticus and coma in a healthy young adult. | | 1995 | 7529901 |
| from the centers for disease control and prevention. encephalitis associated with cat scratch disease--broward and palm beach counties, florida, 1994. | | 1995 | 7531250 |
| dual role for afipia felis and rochalimaea henselae in cat-scratch disease. | | 1995 | 7531265 |
| detection of rochalimaea henselae dna by polymerase chain reaction from suppurative nodes of children with cat-scratch disease. | a polymerase chain reaction (pcr) assay was designed to amplify dna from rochalimaea henselae, rochalimaea quintana and afipia felis in the purulent material from lymph nodes in three patients with clinical cat-scratch disease (csd) and two patients with lymphadenitis from other causes. all of the patients with csd had positive immunofluorescent antibody serology for r. henselae, while none of the controls was positive. pcr amplification confirmed the presence of r. henselae dna and the absence ... | 1994 | 7531320 |
| sinus histiocytosis with massive lymphadenopathy. | | 1995 | 7532075 |
| cat scratch disease: past and present. | | 1994 | 7532423 |
| detection of mip-like sequences and mip-related proteins within the family rickettsiaceae. | the mip surface protein, a prokaryotic analog of the fk506-binding proteins, enhances the ability of legionella pneumophila to infect macrophages and protozoa. using mip-specific probes and low-stringency southern hybridizations, we have detected dna sequences homologous to mip within coxiella burnetii and rochalimaea quintana. using specific anti-mip antisera and immunoblot analysis, we also detected mip-related proteins within these bacteria as well as within rickettsia and ehrlichia species. ... | 1995 | 7532505 |
| recurrent pyogenic granuloma with satellitosis--a localized variant of bacillary angiomatosis? | a 27-year-old male patient had recurrent pyogenic granuloma with satellitosis. histologically, warthin-starry staining of the lesions revealed clumps of dark bacilli as found in patients with bacillary angiomatosis. igg antibodies against bartonella (rochalimaea) henselae were elevated as shown by an indirect immunofluorescence assay. the patient did not present an obvious risk for hiv infection or immunosuppression, and no antibodies against hiv-1 and hiv-2 were found. recurrent pyogenic granul ... | 1994 | 7533011 |
| [bacillary angiomatosis]. | bacillary angiomatosis (ba) is a rare infectious disease usually associated with hiv infection. recent molecular biologic investigations confirm that both rochalimaea henselae and rochalimaea quintana can cause ba. the bacteria can be identified by warthin-starry staining and electron microscopy. the typical clinical signs are solitary or multiple dermal or subcutaneous nodules. bone, liver, spleen and other organs may also be involved. we describe the clinical and histological features of a 39- ... | 1995 | 7533145 |
| [cat scratch (?) disease]. | the case of a 24 year old female student who developed typical cat scratch disease following an insect sting invited to review the literature. over the last two years, rocalimaea henselae could be identified and is now considered to be responsible for the majority of infected cases. it is related to rochalimaea quintana that provokes trench fever and to bartonella bacilliformis, known as the inciting agent of the verruca peruana and of oroya fever. recently, it has been proposed to integrate roc ... | 1995 | 7533323 |
| immunoelectrophoretic characterization and cross-reactivity of rochalimaea henselae, rochalimaea quintana and afipia felis. | the soluble antigens of rochalimaea henselae, rochalimaea quintana and afipia felis were characterized by crossed immunoelectrophoresis using bacterial sonicates as antigens against pooled hyperimmune rabbit sera. a precipitin pattern was drawn for each bacterium and shown to be reproducible and stable even when normal or preimmune rabbit serum was incorporated in the intermediate gel. by this technique 56 antigens were identified from r. henselae, 49 from r. quintana, and 39 from a. felis. the ... | 1994 | 7534092 |
| antibody response in rabbits infected with rochalimaea henselae, rochalimaea quintana and afipia felis. | antibody responses in three pairs of rabbits inoculated with live rochalimaea henselae, rochalimaea quintana and afipia felis were studied by enzyme immunoassay with whole-cell and bacterial sonicates as antigen. no differences in measured antibody responses were found with the two types of antigen preparation. two rabbits did not respond with antibody production. in the remaining rabbits there was a low-titred antibody response that showed no significant cross-reaction with related bacteria. af ... | 1994 | 7534093 |
| [cat-scratch disease and disease caused by bartonella (rochalimaea)]. | the aetiology of cat scratch disease remains controversial since both afipia felis and bartonella (rochalimaea) henselae have been isolated from diseased lymph nodes. bartonella henselae, bartonella (rochalimaea) quintana and bartonella (rochalimaea) elizabethae cause endocarditis and bartonella bacilliformis cause septicemia (oroya's fever) in non-immunocompromized patients, and bartonella henselae and bartonella quintana cause fever, bacillary angiomatosis, and visceral peliosis in human immun ... | 1995 | 7534931 |
| etiology of cat scratch disease: comparison of polymerase chain reaction detection of bartonella (formerly rochalimaea) and afipia felis dna with serology and skin tests. | to determine the role of bartonella (formerly rochalimaea) species and afipia felis in cat scratch disease (csd), two polymerase chain reaction (pcr) hybridization assays were developed to detect dna from these organisms. these assays were applied on 89 pus aspirates from skin test-positive csd patients (group 1) and on 137 pus and lymph node specimens from csd suspects (group 2). bartonella dna was detected in 96% of the samples from group 1 patients and in 60% of group 2 samples; however, a. f ... | 1995 | 7535830 |
| prevalence of antibody to rochalimaea henselae among austrian cats. | | 1995 | 7536676 |
| bacillary angiomatosis and related diseases caused by rochalimaea. | | 1995 | 7536760 |
| cat scratch disease in two children presenting with fever of unknown origin: imaging features and association with a new causative agent, rochalimaea henselae. | to report the clinical course, imaging findings, and method of diagnosis of two patients with systemic manifestations of cat scratch disease, presenting with fever of unknown origin. | 1995 | 7536916 |
| a chemically defined liquid medium that supports primary isolation of rochalimaea (bartonella) henselae from blood and tissue specimens. | rochalimaea (bartonella) henselae is a fastidious, slowly growing, gram-negative bacillus that is an etiologic agent of bacillary angiomatosis, cat scratch disease, and related syndromes. accumulation of direct microbiologic evidence of the relationship between the organism and the syndromes compatible with cat scratch disease has been hindered by the difficulties in the primary isolation of the organism from infected tissue specimens. a chemically defined liquid medium was developed to support ... | 1995 | 7538511 |
| bacillary angiomatosis. | | 1995 | 7539912 |
| pathological case of the month. systemic cat-scratch disease. | | 1995 | 7540915 |
| differentiation of bartonella species using restriction endonuclease analysis of pcr-amplified 16s rrna genes. | differentiation of the four bartonella species which were formerly classified as rochalimaea using restriction endonuclease analysis of pcr-amplified citrate synthase gene fragments has previously been described. however, attempts to extend this method to include all members of bartonella were confounded when amplification of the gene fragment from strains of b. bacilliformis each yielded two products of differing sizes. an alternative differentiation scheme for bartonella species was developed ... | 1995 | 7541758 |
| serologic cross-reactions among ehrlichia equi, ehrlichia phagocytophila, and human granulocytic ehrlichia. | homology in the 16s rdnas shows that the agent of human granulocytic ehrlichiosis (hge) is closely related to the veterinary pathogens erlichia equi and erlichia phagocytophila. after hge, patients develop antibodies reactive with e. equi and e. phagocytophila; thus, we hypothesized that these species are closely related and share significant antigenicity. antisera from humans, horses, dogs, and cattle were tested by indirect fluorescent-antibody assay (ifa) for antibodies reactive with e. equi ... | 1995 | 7542262 |
| use of bartonella antigens for serologic diagnosis of cat-scratch disease at a national referral center. | bartonella henselae (formerly the genus rochalimaea) has recently been isolated from patients with cat-scratch disease and their cats, and since september 1992 the centers for disease control and prevention has offered an indirect fluorescent antibody assay for bartonella-specific antibody. | 1995 | 7542443 |
| cutaneous bacillary angiomatosis in a patient with chronic lymphocytic leukemia. | bacillary angiomatosis is a recently described vascular disorder that is associated with infection by bartonella henselae (formerly known as rochalimaea henselae) and bartonella quintana (formerly known as rochalimaea quintana); this disorder usually occurs in patients with human immunodeficiency virus infection. we report a case of cutaneous bacillary angiomatosis that occurred in a patient with chronic lymphocytic leukemia. | 1995 | 7543264 |
| diagnostic and therapeutic problems due to cat scratch disease. | | 1995 | 7543544 |
| bacillary angiomatosis. | the acquired immunodeficiency syndrome and other causes of immunosuppression have ushered in a variety of opportunistic infections. one of these is bacillary angiomatosis, a vasoproliferative lesion whose principal causative agent is rochalimaea henselae. bacillary angiomatosis, while preponderantly a cutaneous affliction, can be systemic, including involvement of the head and neck mucous membranes. molecular technology and epidemiologic studies used to identify the bacterial agent of bacillary ... | 1995 | 7543738 |
| intraerythrocytic presence of bartonella henselae. | recent reports in the medical literature emphasize the risk of zoonotic disease and the high degree of prevalence of asymptomatic feline infection with bartonella (rochalimaea) henselae. while investigating bartonella bacteremia in cats, we used transmission electron microscopy to demonstrate b. henselae in the erythrocytes of persistently bacteremic cats. | 1995 | 7544366 |
| [perinaud's oculoglandular syndrome as a manifestation of cat-scratch disease]. | the first danish case of parinaud's oculoglandular syndrome, a manifestation of cat-scratch disease, is reported in a 35-year-old man presenting with an enlarged preauricular lymph node and an ipsilateral conjunctival granuloma. surgical removal of the granulomatous lesion was followed by rapid healing. the diagnosis was verified by demonstrating a high antibody tire against rochalimaea (bartonella) henselae. on subsequent questioning the man gave a history of acquiring a kitten six weeks before ... | 1995 | 7544512 |
| prevalence of antibodies to rochalimaea species (cat-scratch disease agent) in cats. | | 1995 | 7544936 |
| differentiation of bartonella-like isolates at the species level by pcr-restriction fragment length polymorphism in the citrate synthase gene. | the citrate synthase gene (glta) of bartonella henselae was cloned and sequenced to compare genetic divergence among alpha and gamma branches of the class proteobacteria and to develop enhanced genotypic reagents for b. henselae identification. b. henselae glta is 1,293 nucleotides in length and 63 to 66% homologous with corresponding gene sequences of rickettsia prowazekii, escherichia coli, and coxiella burnetii. the observed genetic variability suggests that glta sequences can provide a usefu ... | 1995 | 7545181 |
| identification of bartonella (rochalimaea) species among fastidious gram-negative bacteria on the basis of the partial sequence of the citrate-synthase gene. | the bacterial genus bartonella (rochalimaea) includes emerging human pathogens with five recognized species. these are fastidious gram-negative bacteria, exhibiting few phenotypic characteristics and whose identification relies upon serotyping, cellular fatty acid analysis, and molecular typing. most of the isolates have been recovered from the blood of patients, and three of the four pathogenic bartonella species are associated with infectious endocarditis. we performed pcr-restriction fragment ... | 1995 | 7545183 |
| pulmonary manifestations of cat-scratch disease; a case report and review of the literature. | | 1995 | 7545277 |
| bartonella henselae: etiology of pulmonary nodules in a patient with depressed cell-mediated immunity. | we describe an immunocompromised renal transplantation patient with opportunistic lung infection due to bartonella henselae (formerly rochalimaea henselae) and provide evidence suggesting transmission from a pet cat. computed tomographic scans of the chest and lung biopsies provided material for diagnosis. the etiology was established by polymerase chain reaction and sequencing of a 16s ribosomal dna segment from infected lung tissue. histopathologic and serological evidence supported the molecu ... | 1995 | 7548500 |
| neurologic complications of bartonella henselae infection. | bartonella henselae can cause cat scratch disease and bacillary angiomatosis, a multisystem disorder seen primarily in patients with the acquired immunodeficiency syndrome. both of these diseases are associated with neurologic complications, particularly encephalopathy. b. henselae may also cause bacteremia and endocarditis, and has been associated with aseptic meningitis and with dementia in patients also infected with the human immunodeficiency virus. recent advances in identification of this ... | 1995 | 7551113 |
| cutaneous manifestations of opportunistic infections in patients infected with human immunodeficiency virus. | bacillary angiomatosis (ba) presents most commonly as a cutaneous disease and is caused by two organisms. bartonella (rochalimaea) henselae and bartonella (rochalimaea) quintana. biopsy confirmation of cutaneous ba is essential because lesions can mimic nodular kaposi's sarcoma in appearance. although the vast majority of human immunodeficiency virus (hiv)-infected patients with ba have cd4 lymphocyte counts of less than 100 cells per mm3, the disease responds well to antimicrobial therapy. stap ... | 1995 | 7553576 |
| nucleotide sequence analysis of the 23s ribosomal rna-encoding gene of bartonella bacilliformis. | we report the cloning and characterization of the 23s ribosomal rna (rrna)-encoding gene (rdna) of bartonella bacilliformis (bb). the 2821-bp gene is preceded by an 11-nucleotide (nt) inverted repeat (ir) located 81 nt upstream in the trna(ala)-23s rdna intergenic spacer. the gene is followed by an 8-nt ir, five nt downstream in the 23s-5s rdna intergenic spacer. the nt sequence of the bb 23s rdna is most similar to the 23s rdna of rhodopseudomonas palustris (rp), with 85.4% sequence identity. t ... | 1995 | 7557421 |
| cloning, sequencing, and phenotypic analysis of laf1, encoding the flagellin of the lateral flagella of azospirillum brasilense sp7. | azospirillum brasilense can display a single polar flagellum and several lateral flagella. the a. brasilense sp7 gene laf1, encoding the flagellin of the lateral flagella, was isolated and sequenced. the derived protein sequence is extensively similar to those of the flagellins of rhizobium meliloti, agrobacterium tumefaciens, bartonella bacilliformis, and caulobacter crescentus. an amino acid alignment shows that the flagellins of these bacteria are clustered and are clearly different from othe ... | 1995 | 7559324 |
| strategy to detect and identify bartonella species in routine clinical laboratory yields bartonella henselae from human immunodeficiency virus-positive patient and unique bartonella strain from his cat. | we wished to develop a cost-effective, rapid strategy to detect and identify bartonella species in the clinical laboratory and to determine the prevalence of bartonella infection in the houston veteran population. bartonella colonies were identified by colony morphology, gram stain, rapid ana, repetitive extragenic palindromic-pcr (rep-pcr) and whole-cell fatty acid (cfa) analysis, and these methods were compared for their usefulness. a new test order for "rochalimaea culture" (the genus bartone ... | 1995 | 7559957 |
| prevalence of bartonella henselae antibodies in pet cats throughout regions of north america. | cat exposure has been directly associated with the development of human bartonella henselae infections, resulting in cat-scratch disease, bacillary angiomatosis, or bacteremia. the prevalence of serum antibody titers to b. henselae was determined for selected pet cats from 33 geographic locations throughout the united states and several areas in western canada. seroprevalences paralleled increasing climatic warmth (p < .02) and annual precipitation (p < .03). these warm, humid areas with the hig ... | 1995 | 7561200 |
| seroepidemiological survey of bartonella (rochalimaea) henselae in domestic cats in japan. | a total of 199 domestic cat serum samples from 3 geographical areas (northeastern, central and southwestern) of japan collected between 1992 and 1994 were examined for serum antibody against bartonella henselae using an immunofluorescent assay. the antibody prevalence was 15.1% (30/199). a significant difference in the prevalence of b. henselae antibody was observed between the northeastern area (6.3%:3/48) and the central area (22.0:13/59) in japan. there was no significant difference between t ... | 1995 | 7565174 |
| bacillary angiomatosis in patients with aids: multiorgan imaging findings. | to describe the varied clinical manifestations and imaging findings encountered in bacillary angiomatosis, an infectious complication of the acquired immunodeficiency syndrome (aids). | 1995 | 7568856 |
| bartonella infections. evolution from the esoteric. | | 1995 | 7572806 |
| detection of bartonella (rochalimaea) henselae bacteremia using bact/alert blood culture system. | bartonella henselae was isolated from the blood of five febrile immunosuppressed patients using bact/alert (organon teknika, durham, nc) automated microbial detection system. an immunofluorescence assay (using 1:1000 dilutions) was used to confirm identification of fastidious, pleomorphic, non-gram staining, argyrophilic bacilli displaying rachety motility that had been presumptively identified as bartonella spp. the practicality of identification of bartonella henselae using goat antisera for u ... | 1995 | 7572813 |
| [hepatic peliosis, a still current disease]. | | 1995 | 7590002 |
| [peliosis hepatitis during intestinal lymphomatous polyposis treated with chemotherapy and radiotherapy. regression after antibiotic therapy]. | we report a case of regressive peliosis hepatis, which occurred in a 55 year-old woman with diffuse intestinal lymphomatous polyposis in remission after treatment with chemotherapy and radiotherapy, and which was cleared after one month treatment with erythromycin. the warthin-sarry stain, performed to identify a specific agent such as rochalimaea quintana and henselae, was negative, and the polymerase chain reaction technique could not be performed. regressive cases of peliosis hepatis are rare ... | 1995 | 7590008 |
| bartonella henselae and bartonella quintana adherence to and entry into cultured human epithelial cells. | bartonella henselae expresses pili phenotypically similar to type 4 pili. b. henselae pilus expression undergoes phase variation with multiple passages. low-passage-number, piliated b. henselae adhered to and invaded hep-2 cells to a greater extent than did multiply passaged b. henselae with reduced pilus expression. pili may be a pathogenic determinant for bartonella species. | 1995 | 7591104 |
| ocular cat scratch disease. | cat scratch disease (csd) is infectious and can cause a multitude of systemic diseases in addition to ocular csd. rochalimaea henselae has been identified as the causative agent for ophthalmic manifestations of cat scratch disease. a noninvasive method of making a positive diagnosis of ocular csd was recently developed. | 1995 | 7594915 |
| cat-scratch disease in hawaii: etiology and seroepidemiology. | to study the etiology and seroepidemiology of cat-scratch disease (csd) in hawaii. | 1995 | 7608806 |
| genomic fingerprinting of bartonella species by repetitive element pcr for distinguishing species and isolates. | repetitive-element pcr (rep-pcr) with primers based on repetitive extragenic palindromic (rep) and enterobacterial repetitive intergenic consensus (eric) repeated dna sequences was used for genomic finger-printing of bartonella species. this technique was applied by using either extracted genomic dna or preparations of whole bacterial cells directly. pcr fingerprints with either the rep-based primers (rep-pcr) or primers based on the eric repeat (eric-pcr) revealed species-specific band patterns ... | 1995 | 7615711 |
| cat-scratch disease--connecticut, 1992-1993. | a prospective population-based surveillance system was established to characterize the epidemiology of cat-scratch disease (csd) among residents of connecticut who were reported to the state health department with a diagnosis of suspected csd. during 1992 and 1993, 246 persons met the case definition, for an average statewide annual incidence of 3.7/100,000 persons. the median age of patients with csd was 14 years (range, 1-64), and 52% were female. the age-specific attack rate was highest among ... | 1995 | 7622908 |
| inter- and intraspecies identification of bartonella (rochalimaea) species. | species of the genus rochalimaea, recently renamed bartonella, are of a growing medical interest. bartonella quintana was reported as the cause of trench fever, endocarditis, and bacillary angiomatosis. b. henselae has been implicated in symptoms and infections of human immunodeficiency virus-infected patients, such as fever, endocarditis, and bacillary angiomatosis, and is involved in the etiology of cat scratch disease. such a wide spectrum of infections makes it necessary to obtain an intrasp ... | 1995 | 7650189 |
| [a little known cause of persistent fever and granulomatous hepatitis in children: cat scratch disease]. | extensive hepatic and splenic involvement in cat-scratch disease has rarely been reported. | 1995 | 7663655 |
| nucleotide sequence of the 5s ribosomal rna gene of bartonella bacilliformis. | | 1993 | 7680804 |
| rochalimaea elizabethae sp. nov. isolated from a patient with endocarditis. | a rochalimaea-like organism (strain f9251) was isolated from a patient with endocarditis after blood drawn for culture before antimicrobial therapy was subcultured onto blood and chocolate agars and incubated for 2 weeks in 5% co2. the strain was phenotypically similar to known rochalimaea species. the cellular fatty acid composition of strain f9251 was close to but distinct from those of the three known rochalimaea species and was most similar to that of r. vinsonii. labeled dna from strain f92 ... | 1993 | 7681847 |
| phylogenetic analysis of the family rhizobiaceae and related bacteria by sequencing of 16s rrna gene using pcr and dna sequencer. | the 16s rrna gene sequences of 19 strains covering 97% of the molecules were determined for the members of the family rhizobiaceae and related bacteria by pcr and dna sequencer. the three biovars of agrobacterium were located separately, whereas agrobacterium rubi clustered with a. tumefaciens. phylogenetic locations for the species of the genera rhizobium, sinorhizobium, agrobacterium, phylobacterium, mycoplana (m. dimorpha), ochrobactrum, brucella and rochalimaea (a rickettsia) were intermingl ... | 1993 | 7682191 |
| isolation of rochalimaea species. | | 1993 | 7682656 |
| nucleotide sequence and comparison of the 5s ribosomal rna genes of rochalimaea henselae, r.quintana and brucella abortus. | | 1993 | 7685083 |
| characterization of the fmet initiator trna gene of bartonella bacilliformis. | a ribosomal rna operon was cloned from the agent of human oroya fever, bartonella bacilliformis. the 3' end of the operon contains a 77-nucleotide (nt) n-formylmethionine (fmet) initiator trna-encoding gene with 100% sequence identity to the fmet trna-encoding gene of rhodobacter sphaeroides. the 3' region downstream from the gene has an 8-nt inverted repeat and 28 contiguous direct repeats of the heptamer 5'-ttctcta. to date, b. bacilliformis and r. sphaeroides are the only known eubacteria whi ... | 1993 | 7690338 |
| cat scratch disease and bacillary angiomatosis: aetiological agents and the link with aids. | the recent characterisation of a new species of the genus rochalimaea is the culmination of a ten year search for the identity of the agent of bacillary angiomatosis, a condition that is increasingly recognised in association with aids. this work has also implicated rochalimaea henselae in the aetiology of cat scratch disease contact with cats is a risk factor for both conditions. this report describes both diseases and reviews progress toward understanding their microbiology. r. quintana is als ... | 1993 | 7693166 |
| proposed tests for the routine identification of rochalimaea species. | a study was conducted to establish tests for the routine identification of rochalimaea species. strains used were reference strains of rochalimaea vinsonii and rochalimaea quintana, and a type strain and six human isolates of rochalimaea henselae. rochalimaea species were confirmed to be gram-negative, oxidase-negative, non-motile, urease-negative, indole-negative, catalase-negative, glucose-nonfermenting organisms which failed to grow on macconkey agar. further testing of the organisms in a com ... | 1993 | 7694852 |
| purification of deformin, an extracellular protein synthesized by bartonella bacilliformis which causes deformation of erythrocyte membranes. | a factor capable of deforming erythrocyte membranes, found in the culture supernatants of bartonella bacilliformis, was purified 1840-fold using hydrophobic, ion exchange and gel exclusion chromatography. the final fractions contained a single detectable polypeptide species, referred to as deformin, having a molecular weight of 67000 by sds-page and a native molecular weight of 130,000 by gel exclusion chromatography or velocity sedimentation in a glycerol gradient. erythrocytes treated with def ... | 1995 | 7696292 |
| endocarditis in a dog due to infection with a novel bartonella subspecies. | vegetative valvular endocarditis involving the aortic and, to a lesser extent, mitral valves was diagnosed echocardiographically in a 3-year-old spayed female labrador retriever. historically, the dog had been treated with tetracycline hydrochloride and prednisolone for positive seroreactivity to ehrlichia canis and antinuclear antigens. although three aerobic and anaerobic blood cultures failed to grow bacteria, blood cultured simultaneously by the lysis centrifugation technique grew a fastidio ... | 1995 | 7699033 |
| bacillary angiomatosis: a treatable cause of acute psychiatric symptoms in human immunodeficiency virus infection. | bacillary angiomatosis is a systemic infection that has been most commonly reported in the setting of immunosuppression, especially human immunodeficiency virus (hiv) disease. | 1995 | 7713855 |
| the 16s-23s rrna intergenic spacer region of bartonella (rochalimaea) species is longer than usually described in other bacteria. | we amplified by polymerase chain reaction (pcr) and sequenced using an automated laser fluorescent dna sequencer (pharmacia) the intergenic spacer region (its) between the 16s and 23s rrnas of the four species of rochalimaea which were recently renamed bartonella sp. we obtained dna fragments of 1211, 1262, 1258 and 1529 bp for the reference species of b. quintana, b. henselae, b. vinsonii and b. elizabethae, respectively. the its of the four species are longer than previously reported in prokar ... | 1995 | 7737501 |
| a review of bartonellosis in ecuador and colombia. | a review of the literature regarding bartonellosis or carrion's disease in colombia and ecuador is presented, together with observations made by the author in areas of both countries from which the disease has been recorded. there is evidence from pre-columbian artifacts that verruga peruana, the cutaneous form of the disease, was present in ecuador at least 1,000 years prior to the arrival of europeans. these artifacts were discovered in the coastal province of manabi, a low-lying area very dif ... | 1995 | 7741177 |
| [bacillary angiomatosis related to rochalimaea quintana. anatomoclinical and ultrastructural study of cutaneous localizations in aids]. | we report a case of bacillary angiomatosis in a 53-year-old homosexual man with acquired immunodeficiency syndrome (aids). pathological and bacteriological studies of cutaneous nodules led to the identification of a rickettsia: rochalimaea quintana. this observation prompted us to relate the clinical presentation of cutaneous and visceral forms of this disease. histopathological patterns are also considered. they usually consist in a lobular proliferation of capillaries with plump and sometimes ... | 1994 | 7748000 |
| in vitro evaluation of the role of humoral immunity against bartonella henselae. | the contribution of humoral immunity against bartonella henselae was evaluated by examining the in vitro bactericidal activity of sera and the ability of these microorganisms to activate complement and stimulate phagocytosis and an oxidative burst in polymorphonuclear leukocytes. the organism was killed by complement-mediated cytolysis. complement activation preferentially proceeded by the alternative pathway. the presence of specific antibodies did not increase the serum bactericidal activity o ... | 1995 | 7768623 |
| bartonellosis. | | 1995 | 7790311 |
| bartonella (rochalimaea) species as a cause of apparent "culture-negative" endocarditis. | bartonella quintana (formerly rochalimaea quintana) is a recently recognized cause of apparent "culture-negative" endocarditis. we describe a 39-year-old, homeless man who developed aortic valve endocarditis caused by b. quintana. he had a history of alcoholism and was seronegative for the human immunodeficiency virus. we established that b. quintana was the cause of the endocarditis on the basis of the isolation of b. quintana from blood cultures, the compatibility of histochemical stains of ca ... | 1995 | 7795048 |
| lytic vertebral lesions: an unusual manifestation of aids-associated kaposi's sarcoma. | the differential diagnosis of neovascular skin lesions in patients with aids includes kaposi's sarcoma and bacillary angiomatosis. it has been suggested that the radiographic presence of lytic bone lesions in association with these skin lesions supports a diagnosis of bacillary angiomatosis. we present a case of disseminated kaposi's sarcoma in which evidence of lytic vertebral disease was seen on computed tomography; the histopathologic characteristics of the osseous lesions are described. find ... | 1994 | 7803643 |
| bartonella bacilliformis or a similar organism and cardiovascular disease. | bartonella bacilliformis invades the endothelial lining of the cardiovascular system. damage to the red blood cells and white blood cells, the effects of the toxins, invasion of the brain and electrical charges induced by the organism so interfering with normal electrical stimulation of the heart may explain many of the features of cardiovascular disease (1-5). | 1994 | 7815963 |
| [vascular tumors of the skin and soft tissue. overview of newly characterized entities and variants]. | this review summarizes vascular tumours of skin and soft tissues that have been characterised in recent years. although most of them are very rare, knowledge about their reproducible clinicopathological features is important to avoid diagnostic pitfalls. these lesions include: bacillary angiomatosis, a vasoproliferative, pseudoneoplastic infection of immunocompromised patients caused by rochalimaea henselae; tufted angioma, a variant of lobular capillary haemangioma characterised by a "cannon-ba ... | 1994 | 7824435 |
| infectious diseases. | | 1995 | 7838014 |
| proposals to unify the genera grahamella and bartonella, with descriptions of bartonella talpae comb. nov., bartonella peromysci comb. nov., and three new species, bartonella grahamii sp. nov., bartonella taylorii sp. nov., and bartonella doshiae sp. nov. | polyphasic methods were used to examine the taxonomic positions of three newly identified grahamella species. a comparison of the 16s rrna gene sequences of these organisms with the sequences available for other bacteria revealed that these three species form a tight monophyletic cluster with members of the genus bartonella. this cluster is only remotely related to other members of the order rickettsiales. determinations of the levels of dna relatedness between grahamella species and bartonella ... | 1995 | 7857789 |
| evaluation of the automated bact-alert system for pediatric blood culturing. | the organon teknika bact/alert (organon teknika, durham, nc), using the pedi-bact 20 ml aerobic bottle (bpbcs) was compared to the wampole isolator (wi) 1.5 microbial tube (wampole laboratories, cranbury, nj), for detection and recovery of pediatric pathogens. the bpbcs continuously monitors culture bottles for changes in co2 concentrations, while wi cultures are examined twice daily for appearance of colonial growth on agar media. of 5,175 paired blood cultures, 383 pathogens were recovered fro ... | 1995 | 7872254 |
| characterization of a two-gene locus from bartonella bacilliformis associated with the ability to invade human erythrocytes. | bartonella bacilliformis, the agent of human oroya fever, invades erythrocytes and causes a severe hemolytic anemia. the ability of two minimally invasive strains of escherichia coli (dh5 alpha and hb101) to invade human erythrocytes was enhanced 6- to 39-fold by transformation with pial1, a plasmid containing a 1,469-bp bamhi fragment from the b. bacilliformis chromosome. invasiveness was confirmed by gentamicin protection and transmission electron microscopy. dna hybridization analysis confirm ... | 1995 | 7890422 |
| bartonella (rochalimaea) quintana isolation in patient with chronic adenopathy, lymphopenia, and a cat. | | 1994 | 7909030 |
| identification and characterization of a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis. | a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. the gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kda in m. a. paratuberculosis. the clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative se ... | 1994 | 7921248 |
| [cat scratch disease]. | cat scratch disease (csd) is usually manifested in children and young adults as a benign, chronic self-limited lymphadenopathy. however, various atypical presentations have been described, especially in immunocompromised hosts. 1 of 5 cases of csd diagnosed here during 1985-1993 is presented. in 3 cases the diagnosis was supported by lymph node biopsy in which the organisms were visualized by warthin-starry silver impregnation staining. recent publications indicate that afipia felis and rochalim ... | 1994 | 7927015 |
| [recognition and identification of unknown infectious agents]. | the recognition and the identification of previously unrecognized infectious agents require a multidisciplinary approach to specify the nosologic entity of the disease and the epidemiological data, especially the modes of transmission and the risk factors, as well as to discover the microorganism in the laboratory. in the past 20 years, significant breakthroughs have been achieved in cellular cultures (growth factors), in immunology (monoclonal antibodies), and moreover in molecular biology, whi ... | 1994 | 7939294 |