Publications

persistence of anaplasma marginale (rickettsiales: anaplasmataceae) in male dermacentor andersoni (acari: ixodidae) transferred successively from infected to susceptible calves.	the persistence of anaplasma marginale theiler in male dermacentor andersoni stiles ticks exposed to the organism as adults was studied as the ticks were successively transferred to five susceptible calves. all calves fed upon by these ticks rapidly developed clinical anaplasmosis; incubation periods of infection ranged from 19 to 26 d and did not change significantly with successive feedings. development of a. marginale in tick midgut and salivary glands was followed daily during tick feeding ( ...	1992	1495076
anaplasma marginale: failure of sera from immune cattle to confer protection in passive-transfer experiments.	high levels of immunity to anaplasma marginale were induced in cattle either by vaccination using sonically disrupted a. marginale-infected erythrocytes or by repeated infection with different strains of the rickettsia. in both instances, high levels of anti-a. marginale antibody were detected in the sera of the immune cattle by immunoblotting. serum from one animal that had been made immune by repeated infection was transferred intravenously to a. marginale-susceptible calves (three non-splenec ...	1992	1495919
a specific dna probe which identifies babesia bovis in whole blood.	a genomic library of babesia bovis dna from the mexican strain m was constructed in plasmid pun121 and cloned in escherichia coli. several recombinants which hybridized strongly to radioactively labeled b. bovis genomic dna in an in situ screening were selected and further analyzed for those which specifically hybridized to b. bovis dna. it was found that pmu-b1 had the highest sensitivity, detecting 25 pg of purified b. bovis dna, and 300 parasites in 10 microliters of whole infected blood, or ...	1992	1496779
serologic survey for selected arboviruses and other potential pathogens in wildlife from mexico.	during 1988 and 1989, a serologic survey of wildlife was conducted in northeastern mexico to determine the presence, prevalence, and distribution of arboviruses and other selected disease agents. eighty mammal specimens were tested. antibodies to vesicular stomatitis-indiana, venezuelan equine encephalitis-mena ii, rio grande virus, and vesicular stomatitis-new jersey were detected predominantly in small mammals. deer and mouflon (ovis musimon) had antibodies to bluetongue and epizootic hemorrha ...	1992	1512876
primary fibrinogenolysis complicating second trimester placental separation.	we present a case of second trimester placental separation complicated by severe bleeding diathesis. primary fibrinogenolysis is suggested as the cause of the coagulopathy.	2006	1550391
fistula injury to the bladder at repeat cone biopsy by laser.	a 33-year-old woman underwent repeat cone biopsy of the cervix with a carbon dioxide (co2) laser. at operation the laser caused multiple full thickness fenestrations of the bladder, creating multiple small vesico-vaginal fistulae. the cone biopsy was completed and the fistulae were converted into a single defect which was immediately repaired. the patient went home 'dry'. we believe this is the first such report in the english-language literature.	2006	1563577
[why do physicians and patients ever more often resort to practitioners of alternative medicine: the healer and the quack?].		2006	1563606
a cloned dna probe for cowdria ruminantium hybridizes with eight heartwater strains and detects infected sheep.	the dna probe pcs20, which was cloned from the dna of the crystal springs heartwater strain from zimbabwe, cross-reacted with dnas of heartwater strains from all endemic areas, including four heartwater strains from zimbabwe, two strains from south africa, one strain from nigeria, and the gardel strain from the caribbean island of guadeloupe. by nucleic acid hybridization, the pcs20 dna probe detected cowdria ruminantium dna in all dna preparations made from plasma samples from infected sheep be ...	1992	1572987
phylogenetic relationship of cowdria ruminantium, agent of heartwater, to anaplasma marginale and other members of the order rickettsiales determined on the basis of 16s rrna sequence.	the phylogenetic relationship between cowdria ruminantium and representative members of the orders rickettsiales and chlamydiales has been examined on the basis of the sequence of the 16s rrna. phylogeny reconstruction by using both parsimony and distance methods supports the conclusion that c. ruminantium is closely related to the rickettsiales and in particular to the family anaplasmataceae. a signature of nine base substitutions delineated the linkage of anaplasma marginale with c. ruminantiu ...	1992	1581187
development of anaplasma marginale in male dermacentor andersoni transferred from parasitemic to susceptible cattle.	the development and transmission of anaplasma marginale was studied in dermacentor andersoni males. laboratory-reared male d andersoni were allowed to feed for 7 days on a calf with ascending a marginale parasitemia. the ticks were then held in a humidity chamber for 7 days before being placed on 2 susceptible calves. anaplasmosis developed in the calves after incubation periods of 24 and 26 days. gut and salivary glands were collected from ticks on each day of the 23-day experiment and examined ...	1992	1586018
[digestive hemorrhage caused by aorto-duodenal fistula. report of 5 cases].	the aortoduodeonal fistula is a rare condition and has high mortality. we report five retrospective cases of digestive hemorrhage due to an aortoduodenal fistula (4 secondary and 1 primary) between 1986 and 1990. three of them died. attention is called to the importance of clinical suspicion and the main diagnostic methods in early diagnosis such as oral endoscopy. we also enhance the importance of emergency surgery for survival.	2006	1599763
seroprevalence survey for anaplasma marginale-infection of austrian cattle.	a serologic survey study of 5,076 austrian cattle farming herds was carried out in the period of december 1988 till march 1990. one animal was randomly selected from each herd and the antibody titer against anaplasma marginale in blood serum samples was evaluated by means of the complement fixation test. the number of these tested blood samples was 3.6% of 140,081 cattle herd farms of austria. 109 (2.1%) of the tested animals showed positive titers (1:10) against anaplasma marginale, in relation ...	1992	1621479
detection of babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite dna.	carrier cattle infected with babesia bovis are difficult to detect because of the low numbers of parasites that occur in peripheral blood. however, diagnosis of low-level infections with the parasite is important for evaluating the efficacies of vaccines and in transmission and epidemiological studies. we used the polymerase chain reaction (pcr) to amplify a portion of the apocytochrome b gene from the parasite and tested the ability of this method to detect carrier cattle. the target sequence i ...	1992	1624551
preliminary attempts to infect bovine erythrocytes in vitro with a tick-derived stage of anaplasma marginale.		1992	1626895
influence of a menhaden oil diet on cercarial penetration of schistosoma mansoni.	the ability of a menhaden oil (mo) diet to influence cercarial penetration into mouse tail skin was evaluated. male cd-1 mice 4-6 wk old (15.2 g average weight) were fed a 0, 10%, or 20% mo-supplemented diet for 2 wk. after this time mice were infected with either 65 +/- 3 or 145 +/- 3 [35s]methionine/cysteine-labeled cercariae for 1 hr by tail immersion. twenty-four hours and 7 days later groups of mice were killed and their tail skin removed and autoradiographed. at 24 hr postinfection, mice f ...	1992	1635035
responses of gastrointestinal peptides and motor activity to milk and water feedings in preterm and term infants.	because duodenal motor activity differs between preterm and term infants during fasting, this study evaluated the responses of motor activity and peptide release in response to feeding. in the first study, fasting concentrations of gastrin, gastric inhibitory peptide, neurotensin, and peptide yy (pyy) were determined in 53 preterm and 20 term infants. plasma concentrations of gastrin and neurotensin were significantly lower in preterm infants than in healthy adults reported previously by our lab ...	2006	1635821
time course of cerebral blood flow and histological outcome after focal cerebral ischemia in rats.	the relation between time-dependent changes in cerebral blood flow and the appearance of infarction after focal cerebral ischemia is still a matter for debate. the aim of this study was to measure perfusion after simultaneous occlusions of the left middle cerebral artery and ipsilateral common carotid artery in rats and correlate it with the timing and distribution of histological changes.	1992	1636189
network of signal transduction pathways involving lipids: protein kinase c-dependent and -independent pathways.		2006	1636498
the msp1 beta multigene family of anaplasma marginale: nucleotide sequence analysis of an expressed copy.	a gene for the beta subunit of the immunoprotective surface antigen msp-1 of anaplasma marginale was previously cloned and expressed in escherichia coli. a nucleic acid probe based on this gene detects a. marginale infection in carrier cattle and in the tick vector. we report here the sequence and structural features of the cloned msp1 beta gene and expressed polypeptide. the gene codes for a polypeptide of 756 amino acids that contains domains of tandemly repeated sequence and glutamine-rich re ...	1991	1671779
molecular basis for surface antigen size polymorphisms and conservation of a neutralization-sensitive epitope in anaplasma marginale.	anaplasmosis is one of several tick-borne diseases severely constraining cattle production and usage in many parts of the world. cattle can be protected from anaplasmosis by immunization with major surface protein 1, a surface protein of anaplasma marginale carrying a neutralization-sensitive epitope. marked size polymorphisms exist among different isolates of a. marginale in the amf105 subunit of major surface protein 1, yet all isolates still contain the neutralization-sensitive epitope. to cl ...	1990	1691504
in vivo staining of mouse preimplantation embryos with hoechst 33342.	female mice bearing secondary oocytes or preimplantation embryos of various stages were injected with hoechst 33342 (h 33342), either intravenously or intraperitoneally and sacrificed at various intervals afterward. the dye penetrated through the wall of oviduct and stained the nuclei of its cells as well as mitotic chromosomes of secondary oocytes, the pronuclei of one-cell embryos, and nuclei of blastomeres and polar bodies. the nuclear fluorescence was observed in living cells flushed out of ...	1990	1695497
identification of a theileria mutans-specific antigen for use in an antibody and antigen detection elisa.	purified piroplasms of theileria mutans were used to immunize balb/c mice to generate monoclonal antibodies (moabs). the moabs recognized an antigen of a relative molecular mass of 32 kda in western blots. this antigen was also recognized by sera from cattle which had recovered naturally from experimental tick-transmission or infections induced by the blood stages of t. mutans. the moabs did not react, in indirect immunofluorescence or enzyme-linked immunosorbent assays (elisa), with the common ...	1990	1698274
analysis of protein compositions and surface protein epitopes of anaplasma centrale and anaplasma marginale.	protein composition was compared and epitopes were analyzed among the isolates of anaplasma centrale and a. marginale by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting using bovine antisera and monoclonal antibodies, and enzyme-linked immunosorbent assay. common and unique proteins were found among the isolates. all isolates tested had a major surface protein with an apparent molecular weight of 38 to 40 kilodalton which had slight molecular size variations between spe ...	1991	1713792
induction of protective immunity by using anaplasma marginale initial body membranes.	anaplasma marginale initial bodies of the norton zimbabwe strain were disrupted and separated into two membrane fractions banding at 1.15 and 1.22 g/cm3 by sucrose density centrifugation. the membrane fractions differed in their morphology and polypeptide composition. membranes banding at 1.22 g/cm3 shared epitopes with surface-exposed polypeptides of the florida strain of a. marginale, confirming the outer membrane location of these polypeptides. immunization of cattle with either membrane frac ...	1991	1715323
comparison of commercially available cytokeratin antibodies in normal and neoplastic adult epithelial and non-epithelial tissues.	five commercially available cytokeratin antibodies (lu-5, ae1/ae3, cam 5.2, mfn116 and anti-cytokeratin 18) were used to stain a wide range of normal and neoplastic epithelial and non-epithelial tissues to assess their potential value in diagnostic histopathology. all five showed good specificity, with some cross-reactivity in smooth muscle cells. the wider reactivity of ae1/ae3, lu-5, and mfn 116, which includes cytokeratins 8,18 (moll's catalogue) expressed in simple epithelia and their tumour ...	2007	1716266
crossprotective immunity between the florida and a zimbabwe stock of anaplasma marginale.	cattle immunised by infection with the florida stock of anaplasma marginale were protected against subsequent homologous challenge, as demonstrated by complete prevention of microscopically detectable parasitaemia. identically immunised cattle were partially protected against challenge with the norton, zimbabwe stock of a. marginale as determined by the significant prolongation of the prepatent period, a significantly lower peak parasitaemia, and a significantly lower percentage drop in haemoglo ...	1991	1724581
acute myocardial infarction after blunt chest trauma in young people: need for prompt intervention.	we describe four cases of acute myocardial infarction in young patients, secondary to blunt chest trauma. one case was treated with intracoronary thrombolysis and angioplasty, two cases received systemic thrombolysis, and the last one did not have any reperfusion therapy. the coronary angiograms of the left anterior descending artery showed thrombosis in two cases, coronary dissection in one case, and no morphological lesions in the other. we encourage the early performance of angiographic studi ...	2006	1764738
mass screening of cattle sera against 14 infectious disease agents, using an elisa system for monitoring health in livestock.	mass screening elisa methods were developed for testing cattle serum for antibodies against 14 common livestock diseases simultaneously. the absorbance values were transformed to a %elisa (spectrophotometric antibody end point) by a computer interfaced with a microplate reader. a histogram indicating a cutoff point and a report for the veterinarian also was generated. the computer program produced a print-out of the antibody profile for each animal tested, the antibody concentration against each ...	1991	1767993
development of an in vitro microtest to assess drug susceptibility of babesia bovis and babesia bigemina.	continuous cultivation of the bovine hemoparasites babesia bovis and babesia bigemina was developed as an in vitro microtest to assess parasite susceptibility to babesicidal compounds. reproducibility of parasite multiplication rates was independent of culture size, making it possible to use a microscale of 100 microliters for each test sample. inhibitory concentrations (ic50s) of a commonly used babesicide, quinuronium sulfate, evaluated by this in vitro method were found to be 5 x 10(-8) g/ml ...	1991	1779305
microsomal activation of benzo[a]pyrene by alligator mississippiensis: mechanisms, mutagenicity and induction.		1991	1783208
preliminary observations on ticks and tick-borne diseases in the north west province of cameroon. i. babesiosis and anaplasmosis.	during a survey on ticks and tick-borne diseases in the north-western cameroon at the bamenda cattle market, the ticks identified were boophilus annulatus (20%) and b. decoloratus (80%). more than 50% of the ticks were collected during the dry season. of 524 blood smears 47.3% were positive for babesia bovis, 31.1% for b. bigemina and 2.2% for anaplasma marginale. 19.4% were negative.	1991	1824132
efficacy and tolerability comparison of etodolac and piroxicam in the treatment of patients with osteoarthritis of the knee.	the efficacy and tolerability of etodolac and piroxicam were compared in patients with osteoarthritis of the knee. two hundred and twenty patients entered a double-blind, parallel group trial and were randomly assigned to receive 300 mg etodolac twice daily (n = 112) or 20 mg piroxicam once daily (n = 108) for 8 weeks. the etodolac group showed significant improvement (p less than 0.05) from baseline in all efficacy assessments at all evaluations. the piroxicam group showed improvement from base ...	2006	1828415
efficacy and tolerability comparison of etodolac and piroxicam in the treatment of patients with osteoarthritis of the knee.	the efficacy and tolerability of etodolac and piroxicam were compared in patients with osteoarthritis of the knee. two hundred and twenty patients entered a double-blind, parallel group trial and were randomly assigned to receive 300 mg etodolac twice daily (n = 112) or 20 mg piroxicam once daily (n = 108) for 8 weeks. the etodolac group showed significant improvement (p less than 0.05) from baseline in all efficacy assessments at all evaluations. the piroxicam group showed improvement from base ...	2006	1828415
candida albicans-specific ly-2+ lymphocytes with cytolytic activity.	to determine whether antigen (ag)-specific cytotoxic t lymphocytes are generated during experimental candida albicans infection, purified l3t4+ and ly-2+ lymphocytes from immunized mice were cultured in the presence of syngeneic accessory cells, c. albicans ag, and interleukin 2. yeast-infected bone marrow macrophages were used as target cells in a standard 51cr-release assay. freshly isolated l3t4+ and ly-2+ lymphocytes failed to lyse either target cell type. however, ag-specific, major histoco ...	1991	1828430
candida albicans-specific ly-2+ lymphocytes with cytolytic activity.	to determine whether antigen (ag)-specific cytotoxic t lymphocytes are generated during experimental candida albicans infection, purified l3t4+ and ly-2+ lymphocytes from immunized mice were cultured in the presence of syngeneic accessory cells, c. albicans ag, and interleukin 2. yeast-infected bone marrow macrophages were used as target cells in a standard 51cr-release assay. freshly isolated l3t4+ and ly-2+ lymphocytes failed to lyse either target cell type. however, ag-specific, major histoco ...	1991	1828430
[development of public health service with special reference to health monitoring of the drinking water supply in east germany since 1945, with examples from the gera district].	government-controlled hygienic inspection was a part of public health in the german democratic republic that developed into a uniformly all-embracing, active, controlling and executive public instrument also in the field of hygienic control and monitoring of drinking water supply. the article describes the pros and cons of centralistic principles, especially with regard to controlling government-run enterprises and institutions by government-conducted control organisations. the problems involved ...	2007	1828552
[opinions and expectations of physicians of the halle district for restructuring health care].	the article reports on a sociological investigation on physicians' views and expectations regarding the restructuring of the east german health system. 1,420 physicians of a former gdr district (= some 3.5 per cent of gdr doctors) were questioned. the data were ascertained in april 1990 via questionnaire.	2007	1828556
altered coupling states between calcium transport and (ca2+, mg2+)-atpase in the as-30d ascites hepatocarcinoma plasma membrane.	plasma membrane fractions from normal, regenerating liver and the as-30d ascites hepatocarcinoma exhibited a high degree of enrichment when a set of plasma membrane enzyme markers were studied in comparison to the ones associated to the mitochondrial and cytosolic compartments. while the (ca2+, mg2+)-atpase observed for the plasma membrane fraction isolated from normal liver showed an activity of 1.2 mumoles/mg/min, the regenerating liver and the as-30d plasma membrane fractions presented a much ...	1991	1828860
trypsin modification of phosphofructokinase from ascaris suum.	phosphofructokinase from ascaris suum is a tetramer with subunits of 90 kda. treatment of the native enzyme with trypsin (10%, w/w) followed by sds-gel electrophoresis was shown to immediately generate a 40-kda fragment followed by a gradual formation of two other fragments of 37 and 32 kda. the loss of catalytic activity during the digestion was less than 50%. gel filtration of the digested enzyme under non-denaturing conditions showed a mr almost that of the native enzyme. digestion of the pho ...	1991	1828862
the molecular basis of the hepatic microsomal glucose-6-phosphatase system.		2001	1850300
regulation of alcohol consumption by the renin-angiotensin system: a review of recent findings and a possible mechanism of action.	the renin-angiotensin system has traditionally been associated with the regulation of fluid and electrolyte balance. in this review we summarize the data which ascribes a completely new function to this system, i.e., the regulation of alcohol consumption. in addition, we suggest a possible mechanism for this effect based on the concept of a satiety or stop process. the approach taken was to examine the effect on alcohol intake of a wide variety of drug, genetic, dietary, surgical and neurosurgic ...	2007	1852316
determination of ring- and n-substituted amphetamines as heptafluorobutyryl derivatives.	an improved derivatization method for analysing 12 ring- and n-substituted amphetamine-derivatives in body fluids or seized materials by gas chromatograph combined either with mass spectrometer, electron capture or nitrogen phosphorus detector is presented. heptafluorobutyric anhydride is used as derivatization reagent. no heating or standing period is needed in this procedure. most of the drugs considered were distinguishable from each other according to their retention times and all of them ac ...	2006	1855720
determination of ring- and n-substituted amphetamines as heptafluorobutyryl derivatives.	an improved derivatization method for analysing 12 ring- and n-substituted amphetamine-derivatives in body fluids or seized materials by gas chromatograph combined either with mass spectrometer, electron capture or nitrogen phosphorus detector is presented. heptafluorobutyric anhydride is used as derivatization reagent. no heating or standing period is needed in this procedure. most of the drugs considered were distinguishable from each other according to their retention times and all of them ac ...	2006	1855720
signal detectability: the use of roc curves and their analyses.	issues related to roc curves are addressed. the original article on the subject by lee lusted, describing the "state of the art" 20 years ago, is reviewed. the concepts that lusted addressed are then expanded, suggesting the current state of the art. new issues that have arisen with regard to roc curves and their use in medicine are addressed. finally, potential areas for future investigation are suggested.	2007	1865776
lovastatin inhibits gallstone formation in the cholesterol-fed prairie dog.	the efficacy of lovastatin, an inhibitor of hepatic cholesterol synthesis in the prevention of cholesterol gallstone formation, was evaluated in the prairie dog model. two groups of animals were maintained on either nonlithogenic or 1.2% cholesterol-enriched chow for 21 days. seven of the animals in each group received lovastatin, and the remaining six received only distilled water. all of the cholesterol-fed/water-treated animals had crystals and 83% had gallstones, but none of the cholesterol- ...	1991	1867522
in situ activation of syngeneic tumour-specific cytotoxic t lymphocytes: intra-pinna immunization followed by restimulation in the peritoneal cavity.	tumour-specific cytotoxic t lymphocytes (ctl) are usually obtained after immunization in vivo and restimulation of immune cells in vitro. we here describe the generation of syngeneic tumour-specific ctl within no more than 9 days by priming and restimulation in vivo. this is achieved only if the correct sites are used both for primary immunization (ear pinna) and for restimulation (peritoneal cavity). the kinetics of immune t cell induction and of the secondary response in vivo will be reported. ...	2006	1868488
in situ activation of syngeneic tumour-specific cytotoxic t lymphocytes: intra-pinna immunization followed by restimulation in the peritoneal cavity.	tumour-specific cytotoxic t lymphocytes (ctl) are usually obtained after immunization in vivo and restimulation of immune cells in vitro. we here describe the generation of syngeneic tumour-specific ctl within no more than 9 days by priming and restimulation in vivo. this is achieved only if the correct sites are used both for primary immunization (ear pinna) and for restimulation (peritoneal cavity). the kinetics of immune t cell induction and of the secondary response in vivo will be reported. ...	2006	1868488
allergic reaction of the oral mucosa in a patient with a synthetic denture.		2007	1868728
competitive enzyme-linked immunosorbent assay for heartwater using monoclonal antibodies to a cowdria ruminantium-specific 32-kilodalton protein.	hybridomas producing monoclonal antibodies (mab) to cowdria ruminantium were raised. four mabs of the igg isotype reacted in western blots with a 32-kilodalton cowdria protein (cr32), which had previously been shown to be conserved and immunodominant. a fifth mab of the igm isotype recognized a 40-kda cowdria protein. the latter mab was negative in an indirect fluorescent antibody test (ifa), whereas the other four were positive. mab no. 4f10b4 showed the strongest signal in western blots using ...	1991	1871954
use of a most probable number method modified with a deoxyribonucleic probe to monitor control by food preservatives of natural salmonella contamination in animal meat meals.	a most probable number (mpn) technique using a dna probe was developed for determination of salmonella in naturally contaminated meat meals. the method eliminated the need for 180 selective plates and 75 selective tubes and saved 27 h when compared with a traditional technique; yet it still yielded identical mpn of salmonella per 100 g in five naturally contaminated samples. the method was used to monitor the effect of a commercial food preservative (sal curb) at 0, .25, .50, 1.0, and 2.0% in me ...	1991	1876558
the effects of electrical stimulation, adenosine and adenosine-5'-triphosphate (atp) on mouse rectal muscle.	the effects of electrical field stimulation and of purine compounds, adenosine and adenosine-5'-triphosphate (atp) were examined on the mouse isolated rectum. electrical field stimulation induced frequency-dependent contractions of mouse rectal muscles which were potentiated by physostigmine and inhibited by atropine or tetrodotoxin. contractile amplitude at 37 degrees c was significantly (p less than 0.05) greater than at 25 degrees c, but the degree of potentiation by physostigmine was signifi ...	1991	1876577
detection of anaplasma marginale rickettsemia prior to onset of clinical signs by using an antigen capture enzyme-linked immunosorbent assay.	an antigen capture enzyme-linked immunosorbent assay was developed by using monoclonal antibodies to conserved epitopes on the anaplasma marginale msp1a surface protein. the assay sensitivity was 1.1 (+/- 0.5)% parasitized erythrocytes, and all infected cattle were detected prior to development of 2.0%-parasitized erythrocytes. positive tests preceded the onset of anemia by a mean of 2 days. the assay was specific for anaplasmosis, as demonstrated by nonreactivity with another common hemoparasit ...	1991	1885753
community health nursing and the aids pandemic: case report of one community's response.	the world health organization (who) currently projects that there may be a cumulative total of 30 million cases of acquired immune deficiency syndrome (aids) worldwide by the year 2000 ("who predicts," 1991). community health nurses (chns), particularly those employed by local and state health departments, have a major role to play in the worldwide public health effort being mounted in response to the aids pandemic. chn roles may include: direct caregiver, advocate, case manager, health educator ...	2007	1885961
effects of environmental exposure on carbon polysulphone composites.	in vivo and in vitro studies were designed to characterize the material degradation associated with implantation of carbon fibre-reinforced polysulphone (c/ps). composite plates were compression moulded for both studies, and a fibre orientation of 0 degrees with the long axis and a fibre volume fraction of approximately 55% were used. the in vitro experiment involved soaking three groups of four plates each at 4, 12 and 26 wk in 0.9% saline solution at 37 degrees c. the plates were measured for ...	1991	1888812
identification of anaplasma marginale long-term carrier cattle by detection of serum antibody to isolated msp-3.	rapid and accurate detection of anaplasma marginale-infected cattle would enhance anaplasmosis control procedures and evaluation of vaccines. current tests based on detection of antibodies in serum are not widely used for several reasons, including the occurrence of either false-positive or false-negative results. we evaluated binding of antibodies in serum to a subunit antigen isolated from a. marginale initial bodies--major surface protein 3 (msp-3). msp-3 was detected in lysates of eight geog ...	1991	1890178
changing trends in surgery for acute cholecystitis.		2001	1891945
an anaplasma centrale dna probe that differentiates between anaplasma ovis and anaplasma marginale dna.	an anaplasma centrale genomic library was constructed in puc13. two clones pac5 and pac137 hybridising to a. centrale and a. marginale dna were isolated from this library. one of these, pac5, also hybridised to dna from a. ovis. the total insert of pac5 was subcloned into pbr322. this subclone, pac5-12, could detect 1 ng a. centrale, 0.5 ng a. marginale and 3.9 ng a. ovis dna. the hybridisation pattern obtained with pac5-12 on digests of a. centrale, a. marginale and a. ovis dna suggests that th ...	1991	1897134
the coexistence of lymphangiosarcoma and kaposi's sarcoma in a renal transplant recipient.	a case of coexisting lymphangiosarcoma and kaposi's sarcoma that occurred in a female renal transplant recipient is presented. both sarcomas were localized to the skin and were slowly progressive over several years. the coexistence of these two sarcomas may indicate that they arose from a common precursor endothelial cell and that systemic immune suppression may be important in the pathogenesis of both these malignancies.	2007	1913470
magnetic resonance imaging in infants exposed to cocaine prenatally: a preliminary report.		2007	1914354
cloning, sequencing, and expression of the p-protein gene (phea) of pseudomonas stutzeri in escherichia coli: implications for evolutionary relationships in phenylalanine biosynthesis.	the phea gene encoding the bifunctional p-protein (chorismate mutase:prephenate dehydratase) was cloned from pseudomonas stutzeri and sequenced. this is the first gene of phenylalanine biosynthesis to be cloned and sequenced from pseudomonas. the phea gene was expressed in escherichia coli, allowing complementation of an e. coli phea auxotroph. the enzymic and physical properties of the p-protein from a recombinant e. coli auxotroph expressing the phea gene were identical to those of the native ...	1991	1919506
significance of the p210 versus p190 molecular abnormalities in adults with philadelphia chromosome-positive acute leukemia.	we investigated the significance of p210 and p190 molecular abnormalities in 32 adults with philadelphia chromosome (ph)-positive acute leukemia. p210 was detected in 15 patients (47%), p190 in 16 (50%), and both in one (3%). p210 was noted in 11 of 24 patients (46%) with acute lymphocytic leukemia, and in four of eight patients (50%) with acute myelogenous or undifferentiated leukemia. among 29 patients with untreated disease (p210, 14 patients; p190, 15 patients), no significant differences in ...	2007	1932753
cadmium content of cameroonian cigarettes: comparison with other foreign brands sold in cameroon.		2007	1932854
parkinson's disease as community health problem: study in norwegian nursing homes. the norwegian study group of parkinson's disease in the elderly.	to examine the extent of under-diagnosis and overdiagnosis of parkinson's disease and to determine quality of treatment in a defined population.	2007	1932932
candida species: emerging hospital bloodstream pathogens.		2007	1940274
identification of immunodominant polypeptides common between anaplasma centrale and anaplasma marginale.	high titered antibody from rabbits immunized with anaplasma centrale or from cattle recovered from a. centrale infection bound predominantly to several 33-36 kda polypeptides present in both a. centrale and the israel-nt isolate of anaplasma marginale. high titered bovine antibody against the israel-nt isolate of a. marginale also reacted predominantly with a. centrale polypeptides in this size range. the immunodominance of the 33-36 kda polypeptides and their cross-reactivity indicate that thes ...	1991	1949581
neuroimaging of cerebral infarction associated with coronary revascularization.		2007	1950903
[cardiac rhythm in healthy newborn infants and in infants from the risk group in the early neonatal period].	studies of heart rhythm parameters, carried out in 88 healthy newborns and in 117 risk-group babies over the course of the early neonatal period (starting from the second minute and up to the seventh day of life) helped derive the normal values of heart rhythm and determine the time characteristics of regulation mechanism establishment in the early postnatal ontogenesis: from sympathetic tonus and centralization of heart rhythm control in the first hours of life to augmenting parasympathetic ton ...	2007	1951972
[pregnancy after transfer of the gametes into the fallopian tubes].	three patients with idiopathic sterility were treated by transferring the gametes into uterine tubes. clomiphene+ citrate stimulation of superovulation was employed. preovulatory oocytes were obtained via the laparoscopic approach. two oocytes with capacitated spermatozoa were placed into the tube of patient 1, four cells into one tube of the second patient, and two cells into each tube of the third patient. pregnancy started in two cases, uterine in one, eventuating in 12-13 weeks in spontaneou ...	2007	1951975
16s ribosomal dna amplification for phylogenetic study.	a set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. one pair of primers is capable of amplifying nearly full-length 16s ribosomal dna (rdna) from many bacterial genera; the additional primers are useful for various exceptional sequences. methods for purification of amplified material, direct sequencing, cloning, sequenci ...	1991	1987160
increased plasma endothelin levels in patients with raynaud's phenomenon.		2007	2008192
efficacy of purified anaplasma marginale initial bodies as a vaccine against anaplasmosis.	anaplasma marginale initial bodies of the florida strain were purified from infected erythrocytes using a combination of ultrasonic disruption, nonionic detergent and differential centrifugation. immunochemical analysis revealed at least 12 a. marginale proteins in the molecular mass (m) range 81-15 kda with a prominent band at 38 kda. several of these proteins remained insoluble in the presence of nonionic detergent. preparations of purified anaplasma initial bodies contained negligible erythro ...	1991	2027888
the homeless shelter. an ideal clinical setting for rn/bsn students.	providing care to persons in homeless shelters may provide an ideal independent nursing experience for students, says witt. here she describes a project that allowed students to use their wits in providing the needed care for this special population.	2007	2034396
ventricular fibrillation in the wolff-parkinson-white syndrome.	ventricular fibrillation (vf) is a well-known but rare complication of the wolff-parkinson-white syndrome (wpw). clinical and electrophysiological data of 23 patients with spontaneous vf were compared with data from 100 consecutive patients with wpw without vf but with symptomatic supraventricular tachycardia. the 23 patients were collected in a multicentre retrospective study in seven european centres. vf occurred in only one patient who was receiving antiarrhythmic drugs, and was the first man ...	2007	2044547
[sudden death syndrome in infants in their first year of life; biological maturity and borderline conditions].		2007	2075054
frozen and fresh anaplasma centrale vaccines in the protection of cattle against anaplasma marginale infection.	the immunity induced by frozen and fresh anaplasma centrale vaccines against anaplasmosis caused by a. marginale was tested in 12-month old friesian steers. a. centrale parasitaemia occurred in all cattle inoculated with both types of vaccine. the average maximal decrease in pcv for the frozen and fresh vaccines was 41.0 and 40.3% respectively. all cattle recovered spontaneously. vaccinated and control steers of the same age were challenged six months later with doses of 10(6), 10(7) or 10(8) a. ...	1990	2092348
parasitic associations with malignant neoplasms.		2007	2100071
interaction between parasite and tick vector.	interaction of tick vectors with borrelia species including b. burgdorferi, rickettsias and piroplasms has been demonstrated by describing selected phenomena. in particular, the various environments inside the tick vector have been considered, including the midgut lumen, gut epithelial cells, body cavities and tissues. intracellular parasitism occurs in different compartments of the host cell: parasitophorous vacuoles (anaplasma marginale), phagolysosomes (coxiella spp.), cytoplasm (rickettsia, ...	1990	2120142
immunodiagnosis of and immunoprophylaxis against the haemoparasites babesia sp. and anaplasma sp. in domestic animals.	the haemoparasites babesia bovis and anaplasma marginale are extremely important pathogens of cattle, affecting more than 300 million animals worldwide. species of less importance affect cattle, dogs, horses and sheep. no vaccine is available for widespread usage. this review summarises existing immunodiagnostic and immunoprophylactic methods currently available for these diseases and reports on future immunoprophylactic methods.	1990	2132684
[natural anaplasma marginale infection in 2 cattle herds with different levels of boophilus microplus tick infestation].	the dynamics of natural infections by anaplasma marginale was studied in two adjacent dairy farms with different levels of boophilus microplus infestation. the farms were located in the enzootic area of bovine anaplasmosis of the northwest of argentina. the study was carried out in 35 calves from birth in march-august 1985 to march 1986. the infection rate by a. marginale was evaluated by the observation of blood films and by determination of specific antibodies. the degree of infestation by b. ...	1990	2132783
[malignant melanoma metastatic to the stomach: upper g-i endoscopic finding--report of a case].	a 54-year-old male was presented with huge, black, ulcerative and painless tumor of the sole of left foot. malignant melanoma was proved by biopsy, but patient refused any treatment. he visited our hospital again when bloody vomitus was noted two months later. chest p-a revealed multiple lung metastases, and marked liver metastasis was also found by abdominal echo. upper gastrointestinal (ugi) panendoscopy revealed multiple melanotic nodules with ulcerations at the tip, scattering diffusely on t ...	2007	2168278
adrenergic receptors and sensitization of adenylyl cyclase in acute myocardial ischemia.	acute myocardial ischemia leads to a gradual increase in beta-adrenergic receptors at the cell surface. this increase occurs rapidly after onset of global ischemia (15 minutes) and persists even after prolonged periods of global ischemia. this alteration can be observed both in vivo and in vitro in isolated perfused hearts. several groups have previously shown that ischemia induces a local release of endogenous catecholamines. here, we show that these endogenous catecholamines are sufficiently h ...	2007	2168300
in vitro cytotoxic effects of cobalt-containing dusts on mouse peritoneal and rat alveolar macrophages.	"hard metal disease" is a chronic lung disease characterized by the presence of interstitial fibrosis; the role of cobalt in the development of this entity is still debated. indeed, most cases have been observed in some workplaces (grinding) of the hard metal industry and in the diamond polishing industry whereas no similar cases have been reported from workers exposed to pure cobalt powders in cobalt refineries. this study was designed to assess the in vitro toxicity (ldh release, morphology) o ...	1990	2168316
the dose rate effects on the in vitro radiolysis products of magnesium-guanosine-5'-monophosphate complexes in aqueous solutions.	this study examined the effects on the radiolysis of magnesium-guanosine-5'-monophosphate complexes in deaerated aqueous solutions, investigated by fourier transform infrared (ft-ir) spectroscopy. it was found that when the system was irradiated with a dose rate of 500 rad/min (low dose rate), the oh radicals, h atoms and hydrated electrons (eaq-) produced from radiolysis of water reacted mainly by addition to the double bonds c4 = c5 and n7 = c8 of guanine. when the dose rate was 23,800 rad/min ...	2007	2168727
mu-opioid receptors mediate the inhibitory effect of opioids on dopamine-sensitive adenylate cyclase in primary cultures of rat neostriatal neurons.	the receptors mediating the inhibition of d1 dopamine receptor-stimulated adenylate cyclase by opioids were examined in primary cultures of rat neostriatal neurons. adenylate cyclase activity was dose-dependently increased by the selective d1 dopamine receptor agonist skf 38393 (ec50 = 0.05 microm). this stimulation was fully antagonized by the selective d1 dopamine receptor antagonist sch 23390 (1 microm). skf 38393 (1 microm)-stimulated adenylate cyclase activity was strongly reduced (by almos ...	1990	2168934
the differentiation of excitability in embryonic chick limb motoneurons.	the well-documented role of neuromuscular activity as a regulator of motoneuron and muscle development raises important questions about the differentiation of excitability in motoneurons. we have recently described changes in expression of voltage-dependent calcium currents that take place during neuromuscular development in the chick embryo (mccobb et al., 1989). we now report similar analyses, using whole-cell patch-recording methods, of the major currents underlying action potential generatio ...	2007	2168941
communication between protein tyrosine and protein serine/threonine phosphorylation.	in the present article, we have reviewed several promising areas of research, the results of which support the existence of enzymatic cascades linking extracellular growth factor receptor/tyrosine kinases with intracellular serine/threonine kinases. proceeding either "upstream" from select serine/threonine kinases or "downstream" from the receptors, characterization of both the distal and proximal elements of these cascades is yielding insights into the multiple pathways of signal transduction. ...	1990	2169797
philippine population development: a nursing concern.	in keeping with nurses' commitment to the preservation and care of human life, population development should be a major concern of the nursing profession in the philippines. the profession should ensure that the health system is oriented toward people's needs and formulate manpower strategies on the basis of these needs and population distribution. nurses are in an ideal position to advocate a family-centered approach to health care through their access to pregnant women and mothers. nurses c ...	1990	2217534
[ticks and hemoparasitic diseases in cattle in senegal. iv. the southern sudan area].	the authors report on the results of an investigation on ticks and hemoparasitoses of cattle, sheep and goats in the south sudanian area of senegal. systematic routine dipping against ticks of cattle, 40 sheep and 40 goats was set during 15 months, with a view to determine the population dynamics together with an acurate localization of the different species concerned. the following parasites were collected from these ruminants: amblyomma variegatum, boophilus geigyi, hyalomma truncatum, h. m. r ...	1990	2218038
the first reported case of hypoglycaemia as the presenting feature of invasive plasmacytoma with a paraprotein band and grossly elevated insulin levels.		2007	2223638
the first reported case of hypoglycaemia as the presenting feature of invasive plasmacytoma with a paraprotein band and grossly elevated insulin levels.		2007	2223638
halophilic proteins and the influence of solvent on protein stabilization.	competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment.	1990	2238041
halophilic proteins and the influence of solvent on protein stabilization.	competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment.	1990	2238041
[effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog].	a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ...	1990	2238764
use of the dot enzyme-linked immunosorbent assay with isolated anaplasma marginale initial bodies for serodiagnosis of anaplasmosis in cattle.	isolated anaplasma marginale initial bodies were successfully used in a dot elisa for rapid detection of antibodies to anaplasma organisms. the enzyme immunoassay used only 25 ng of antigen dotted onto nitrocellulose disks. antigen-antibody complexes were detected by use of alkaline phosphatase-conjugated protein a, and reactions were read visually after addition of a precipitable, chromogenic substrate. the test allowed the processing of multiple sera, either for screening or for titer determin ...	1990	2240769
recombinant dna probe detecting eperythrozoon suis in swine blood.	a genomic library to eperythrozoon suis dna was constructed in lambda gt11, and from this library, e suis clone ksu-2 was identified as a potential diagnostic probe. in hybridization experiments that used 100-microliters samples of blood collected in chaotropic salt solutions, the ksu-2 probe hybridized strongly with purified e suis organisms and blood samples from splenectomized swine that were parasitized with e suis. however, the probe under stringent conditions did not give radiographic indi ...	1990	2240802
rolling chair sign.		2007	2241609
an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies to anaplasma centrale and anaplasma marginale.	an enzyme-linked immunoassay (elisa) was applied to detect antibodies to a. centrale and a. marginale using homologous and heterologous antigens. the assay was compared with the indirect fluorescent antibody (ifa) test, and although a similar degree of sensitivity was obtained, the elisa test had several advantages. partially purified anaplasma initial bodies used for antigen preparations contained negligible amounts of residual erythrocytic material, and did not interfere with the specificity o ...	1990	2247936
consultation section. retrobulbar hemorrhage.		2007	2258817
consultation section. retrobulbar hemorrhage.		2007	2258817
self-screening of blood pressure and sodium in a 24-hour urine sample as part of a school health programme.	a study was conducted to determine the usefulness of self-screening of blood pressure in families as part of a school health care programme, and to study the relationship between bp and sodium excretion in school children. in 750 families with 10-13 year old children, the mothers were instructed to take bp measurements on themselves, their child and the father whilst at home. a comparison of the children's bp measurements at home and at school with those of the parents showed the home measuremen ...	2008	2258868