| detection and quantitation of anaplasma marginale in carrier cattle by using a nucleic acid probe. | cattle which have recovered from acute infection with anaplasma marginale, a rickettsial hemoparasite of cattle, frequently remain persistently infected with a low-level parasitemia and serve as reservoirs for disease transmission. to fully understand the role of these carriers in disease prevalence and transmission, it is essential that low levels of parasitemia can be accurately detected and quantitated. we have developed a nucleic acid probe, derived from a portion of a gene encoding a 105,00 ... | 1989 | 2915021 |
| experimental anaplasmosis in mule deer: persistence of infection of anaplasma marginale and susceptibility to a. ovis. | an experimental anaplasma marginale infection was induced in a splenectomized mule deer (odocoileus hemionus hemionus) which persisted subclinically at least 376 days as detected by subinoculation into susceptible cattle. anaplasma ovis was experimentally transmitted from sheep to a splenectomized and a spleen-intact mule deer, and back to sheep. the pathogenesis in deer was very similar to that seen in sheep using ovine blood inoculations. | 1988 | 3352081 |
| [immunological studies in hamsters (cricetus auratus) infected with schistosoma mansoni]. | | 1986 | 3105038 |
| an enzyme linked immunosorbent assay to diagnose babesia bovis infection in cattle. | the optimum gel filtration fraction from lysate of babesia bovis infected erythrocytes was determined for use as an antigen in an elisa to diagnose b. bovis infection in cattle. of four enzyme labels tested, horseradish peroxidase was the most suitable. the assay is both sensitive and specific in detecting antibody for 2-4 years after a single infection. false positive reactions were obtained only with some sera from some anaplasma marginale infected cattle. | 1987 | 3106957 |
| enzyme-linked immunosorbent assay using solubilised antigen for detection of antibodies to anaplasma marginale. | an enzyme-linked immunosorbent assay (elisa) was used to detect antibodies against anaplasma marginale. a marginale bodies were separated from parasitised erythrocytes by a modified nitrogen decompression method, sonicated, then solubilised with triton x-100 and used as the elisa antigen. in this elisa system the required amount of antigen protein was 16.2 ng for each well. in the course of experimental infections, of calves, significant antibody levels were detected by elisa and the complement ... | 1988 | 3238774 |
| effect of thyroid status and paraventricular area lesions on the release of thyrotropin-releasing hormone and catecholamines into hypophysial portal blood. | trh is a potent stimulator of pituitary tsh release, but its function in the physiological regulation of thyroid activity is still controversial. the purpose of the present study was to investigate trh and catecholamine secretion into hypophysial portal blood of hypothyroid and hyperthyroid rats, and in rats bearing paraventricular area lesions. male rats were made hypothyroid with methimazole (0.05% in drinking water) or hyperthyroid by daily injections with t4 (10 micrograms/100 g bw). untreat ... | 1988 | 3133200 |
| [the effect of long-term exposure to gamma rays (6,7 gy) on the activity of monoamine oxidase in the hypothalamus of sheep during the anestrus period]. | | 1988 | 3146833 |
| creatinine excretion rates for evaluation of kidney function in children. | a protein load protocol for evaluation of kidney function was tested in normal children and pediatric renal patients. an overnight, timed urine collection was used for calculation of the baseline creatinine clearance and creatinine excretion rate. one hour following ingestion of a standardized protein meal (baked chicken), a 2-3 h urine collection was begun. the post-protein meal changes in creatinine clearance showed considerable variation in both the normal children and those with renal disord ... | 1988 | 3153054 |
| radioimmunoassay for anaplasma marginale antibodies in cattle. | a radioimmunoassay is described for use in the detection of anaplasma marginale antibodies in cattle sera. optimal sensitivity and specificity were obtained by using 2 antigens, an a marginale antigen and a rbc antigen (obtained before infection was established) from the same calf. in addition, sera were preabsorbed with rbc from healthy cattle and with sonicated babesia bovis. of 86 sera obtained from cattle with a marginale infection (as determined by blood smear examination or by results of s ... | 1988 | 3377309 |
| activity of ciprofloxacin against resistant clinical isolates. | the in-vitro activity of ciprofloxacin, a new quinolone derivative, was determined by broth microdilution for 518 clinical isolates. the mics for 90% of the organisms were 2 mg/l for pseudomonas aeruginosa, 8 mg/l for p. maltophilia, 4 mg/l for other pseudomonas spp., 1 mg/l for acinetobacter anitratus, 0.03-0.5 mg/l for differing species of enterobacteriaceae, 2 mg/l for streptococcus faecalis, 0.25 mg/l for coagulase-negative staphylococcus spp. and 2 mg/l for s. aureus. furthermore, on a weig ... | 1986 | 2939048 |
| immunological studies in haemophilic children. | a majority of haemophiliacs who have received large-pool plasma products within the past 5 years have been exposed to the putative agent of the acquired immunodeficiency syndrome (aids)--hiv. it is not known what the risk of infection is among patients in south africa. a study was made of 39 children with congenital coagulation disorders attending the red cross war memorial children's hospital haemophilia clinic. all but 3 had been treated exclusively with small-pool lyophilised cryoprecipitate ... | 1987 | 2953077 |
| characteristics of an attenuated anaplasma marginale of deer origin as an anaplasmosis vaccine. | a fully virulent field anaplasma marginale isolate, passaged to a splenectomised white tailed deer, lost virulence for cattle after 18 months in the non-bovine host. this attenuated a. marginale produced a mild response in four 18-month-old holstein steers characterised by an average 2.4% parasitaemia, a 13% drop in pcv and a positive serological response. all vaccinated steers were immune to challenge with a fully virulent, heterologous a. marginale when compared with non-vaccinated controls. | 1988 | 3400115 |
| blood volume regulation. on the right lines at last? | | 1985 | 3157061 |
| verification of ineffectual activity of ivermectin against adult onchocerca spp in the ligamentum nuchae of horses. | ivermectin paste formulation (200 micrograms/kg) was administered orally to 27 horses (13 thoroughbreds and 14 of mixed breeding) to evaluate activity against adult onchocerca spp in the ligamentum nuchae. ages, known or estimated, of the horses ranged from 1 to 22 years. single or multiple doses (1 to 5) of the drug were given to each horse. when multiple doses were administered, the intervals between treatments ranged from 7 to 92 days. at 27 to 171 days after initial treatment (single dose or ... | 1988 | 3421544 |
| specificity of pyoverdine-mediated iron uptake among fluorescent pseudomonas strains. | pyoverdine-mediated iron transport was determined for seven fluorescent pseudomonas strains belonging to different species. for all strains, cell or cell outer membrane and iron(iii)-pyoverdine combinations were compared with their homologous counterparts in uptake, binding, and cross-feeding experiments. for four strains (pseudomonas putida atcc 12633, pseudomonas fluorescens w, p. fluorescens atcc 17400, and pseudomonas tolaasii ncppb 2192), the pyoverdine-mediated iron transport appeared to b ... | 1988 | 3170485 |
| rifampicin-resistant strains of mycobacterium leprae may have reduced virulence. | a strain of mycobacterium leprae resistant to rifampicin (rmp) failed to infect normal mice when injected into the foot pads (fp) at a dose of 10 or 100 bacilli/fp, although it could be maintained by serial passage in mice by the use of inocula of 10(4) bacilli/fp; normal mice can be infected by rmp-sensitive m. leprae at a dose of 10 bacilli/fp. by contrast, nude (athymic) mice could be infected with an inoculum of 10 bacilli/fp of the rmp-resistant strain. it is suggested that the strain conce ... | 1988 | 3275779 |
| hybridization of dna probes to a. marginale isolates from different sources and detection in dermacentor andersoni ticks. | dna from the washington, south-idaho, virginia and florida isolates of anaplasma marginale was hybridized to probes specific for anaplasma centrale and a. marginale. the a. centrale probes ac-2 and ac-4 hybridized to identical bands on all of these isolates. the hybridization patterns suggests that the virginia, florida and the south african isolates are similar. a number of bands were obtained with the washington isolate which differed from those obtained with the other isolates. probe ac-2 cou ... | 1988 | 3217095 |
| prevalence of anaplasmosis and babesiosis in n'dama cattle of the gambia. | sera from 184 n'dama cattle randomly selected and averaging 2-7 years of age were tested for the presence of specific antibodies to anaplasma marginale, babesia bovis and b. bigemina, using one or more serological tests including complement fixation, rapid card agglutination and indirect fluorescent antibody (ifa). tests for a. marginale and b. bovis were essentially negative. utilising the ifa test 65% of the sera tested were positive for b. bigemina. three randomly selected two-year-old n'dama ... | 1988 | 3281338 |
| hepatitis d in new zealand preliminary survey. | | 1986 | 3459096 |
| prevalence of cytomegalovirus antibodies in thai blood donors. | antibodies to cytomegalovirus (cmv) were determined in thai blood donors using the complement fixation (cf) test and enzyme-linked immunosorbent assay (elisa). a total of 203 voluntary blood donors, 181 males and 22 females, who came to the blood bank at siriraj hospital during february 1985, were investigated. their ages ranged from 17 to 53 years (mean 24.3 +/- 6.9). seventy-three out of 156 (46.8%) and 171 out of 203 (84.2%) sera were positive for cmv antibodies as detected by the cf test and ... | 1985 | 3000401 |
| further evidence that a beta-adrenergic mechanism regulates water intake: role of the subfornical organ. | the daily water intake was reduced in rats by subcutaneous administration of propranolol. the fluid intake after 24 h of dehydration in nephrectomized animals was decreased by propranolol. in intact rats diazepam did not modify the postdehydration water intake. propranolol injected into subfornical organ decreased water consumption after 24 h of fluid deprivation but did not change the dipsogenic response to carbachol given in the same structure. these results suggest that a beta-adrenoceptor me ... | 1986 | 3019711 |
| further evidence that a beta-adrenergic mechanism regulates water intake: role of the subfornical organ. | the daily water intake was reduced in rats by subcutaneous administration of propranolol. the fluid intake after 24 h of dehydration in nephrectomized animals was decreased by propranolol. in intact rats diazepam did not modify the postdehydration water intake. propranolol injected into subfornical organ decreased water consumption after 24 h of fluid deprivation but did not change the dipsogenic response to carbachol given in the same structure. these results suggest that a beta-adrenoceptor me ... | 1986 | 3019711 |
| cytotoxic escherichia coli. | | 1987 | 3305326 |
| characterization of mannoproteins from a virulent candida albicans strain and its derived, avirulent strain. | antigenic differences between a wild-type virulent strain of candida albicans 4918 (wt) and its spontaneous avirulent mutant (m-10) were found with crossed-immunoelectrophoresis and western blotting. mannoprotein fractions from yeast and mycelial forms were obtained by affinity chromatography on concanavalin a and analyzed with use of patient sera or sera from rabbits infected with live wt cells and boosted with whole extracts or rabbits immunized with purified wt cell wall preparations. a spike ... | 1988 | 3055210 |
| molecular implications of ph (+) myelodysplastic syndrome. | we report a case of 62-year-old japanese male with a myelodysplastic syndrome (mds) with a philadelphia (ph) chromosome. cytogenetic analysis revealed the bone marrow cells to contain a ph chromosome due to t(?;11;22) (?;q11;q11), as well as -5, -7, +8, -12 and an extra ph, in addition to cells with a normal karyotype. molecular analysis using breakpoint cluster region probes (5' bcr and 3' bcr) did not detect a rearrangement within the bcr dna sequences, indicating that the breakpoint at 22q11 ... | 1988 | 3066168 |
| prevalence of anaplasma marginale, babesia bigemina and b. bovis in nigerian cattle using serological methods. | serum samples collected randomly from 500 cattle from the 10 northern states of nigeria were tested for antibodies against anaplasma marginale by indirect fluorescent antibody (ifa), card agglutination (ct) and capillary tube-agglutination (ca) tests. the serum samples were also examined for antibodies to babesia bigemina and b. bovis by the ifa test only. of the serum samples tested, 79.4% had antibodies against a. marginale by the ifa test, 40 and 25% in the ct and ca tests, respectively. the ... | 1986 | 3538640 |
| immunocytochemical localization of neuropeptide y-like immunoreactivity in adrenergic and non-adrenergic neurons of the rat gastrointestinal tract. | the immunocytochemical location of neuropeptide y (npy)-like immunoreactivity (li) within the neuronal structures of the rat gastrointestinal (gi) tract was investigated with the indirect immunofluorescence method. npy immunoreactive neurons were found throughout all regions of the gi tract with the largest number in the duodenum. npy immunoreactive perikarya were mainly located in the submucosal ganglia. npy labeled processes were extensively seen in the submucosal and myenteric plexuses, smoot ... | 1987 | 3554165 |
| epidemiological patterns and incidence of bio-, sero- and phage types of vibrio cholerae in hyderabad, india, during 1971-1984. | during 1971-1984 out of 44,762 gastroenteritis cases bacteriologically examined, 4240 (9.5%) vibrio cholerae were isolated in hyderabad, india. out of them 1329 (31.3%) were classical and 2911 (68.7%) were el-tor biotypes. the changeover from classical to el-tor cholera was observed in hyderabad during 1975 and persisted. during this 14 year period four major outbreaks with v. cholerae serogroup o1 ogawa, v. cholerae biotype el-tor serogroup o1 ogawa were observed. phage types 1, 2 and 4 were pr ... | 1988 | 3067503 |
| detection of antibodies to anaplasma marginale by an improved enzyme-linked immunosorbent assay with sodium dodecyl sulfate-disrupted antigen. | sensitivities of two enzyme-linked immunosorbent assays (elisas) with particulate and sodium dodecyl sulfate (sds)-disrupted anaplasma marginale antigen were compared. the quotient of positive reference sera divided by the absorbance quotient of a negative reference serum at identical dilution was termed the signal-to-noise ratio. optimal signal-to-noise ratios were dependent on both pretreatment of antigen and antigen concentration. sds disruption of anaplasmal antigen resulted in a markedly im ... | 1987 | 3571472 |
| influence of imidocarb prophylaxis on the pathogenicity and immunogenicity of anaplasma marginale infections in cattle. | | 1987 | 3579755 |
| the genome of anaplasma: dna base composition and dna/dna hybridization. | the tm value of dna from anaplasma centrale and anaplasma marginale was found to be 87.1 degrees c and 89.3 degrees c, respectively. the g + c content, calculated from the tm, was 45.1% for a. centrale and 48.5% for a. marginale. identical hybridization patterns were obtained when the dna from one species was hybridized to restriction endonuclease-digested dna from the other species. | 1987 | 3587929 |
| antibodies against mammary derived growth inhibitor (mdgi) react with a fibroblast growth inhibitor and with heart fatty acid binding protein. | a polypeptide growth inhibitor, designated as mammary derived growth inhibitor, has previously been purified from lactating bovine mammary glands. polyclonal rabbit antiserum raised against mammary derived growth inhibitor cross-reacts with bovine heart fatty acid binding protein and bovine peripheral myelin p2 protein. these results are consistent with the observation that the amino acid sequence of mammary derived growth inhibitor showed homology to the sequences of these proteins. in a parall ... | 1987 | 3318832 |
| interaction of magnesium ion, oxygen tension, and temperature in the production of toxic-shock-syndrome toxin-1 by staphylococcus aureus. | | 1987 | 3102633 |
| medium conditioned by spleen cells of nematospiroides dubius-infected mice does not support development of cultured mast cells. | the ability of different conditioned media to support mast cell development from precursors in normal bone marrow was evaluated. many mast cells developed in bone marrow cultures containing medium conditioned by concanavalin-a-stimulated spleen cells of normal mice, trichinella spiralis-infected mice, or mice infected for 6 days by nematospiroides dubius. by contrast, medium conditioned by concanavalin-a-stimulated spleen cells of mice having 18-day infections of the nematode n. dubius failed to ... | 1988 | 3338850 |
| cultivation of anaplasma marginale from cattle in a dermacentor cell line. | a tick cell line derived from dermacentor variabilis (rml-15) was inoculated with bovine rbc infected with anaplasma marginale. two hours after inoculation, numerous rbc were phagocytized by the tick cells. after one passage of the cell culture, numerous groups of anaplasma-like particles were seen in the tick cell cytoplasm. increased numbers of anaplasma-like particles also were present. seemingly, anaplasma can multiply in tick cells. | 1988 | 3348535 |
| attempted transmission to cattle of anaplasma marginale from overwintered dermacentor andersoni ticks. | since the 1983 summer outbreak of anaplasmosis in southern saskatchewan, the role of the tick, dermacentor andersoni as an overwintering reservoir for anaplasma marginale has been questioned. the purpose of this study was to determine if spring-collected ticks carried virulent a. marginale. sixteen splenectomized calves were assigned randomly to two groups of 14 principals and two controls. adult d. andersoni, collected in april from areas having high transmission rates of a. marginale, were con ... | 1987 | 3651893 |
| ticks parasitising the indian buffalo (bubalus bubalis) and their possible role in disease transmission. | a total of 13 ixodid tick species, boophilus microplus, haemaphysalis bispinosa, haemaphysalis cornupunctata, haemaphysalis himalaya, heamaphysalis montgomeryi, hyalomma anatolicum anatolicum, hyalomma dromedarii, hyalomma marginatum isaaci, hyalomma (hyalommina) brevipunctata, hyalomma (hyalommina) hussaini, nosomma monstrosum, rhipicephalus haemaphysaloides, and r. turanicus were collected off 424 buffaloes from the northwestern states of india. ten tick species, amblyomma testudinarium, b. mi ... | 1988 | 3369083 |
| synthesis of thiazole derivatives with positive inotropic effect. | thiazole derivatives with potential positive inotropic effect were synthesized. the highest activity (medium positive inotropic effect) was exhibited by ethyl 2-(2-hydroxyethylamino)-4-phenylthiazole-5-carboxylate (3b) of which several derivatives have been prepared. among these derivatives only those which could presumably easily removed in the living organism to give 3b exhibited a medium positive inotropic effect (6, 8b). it was assumed that the effect of 3b had a beta-agonist character. | 1987 | 3671455 |
| the primary structure of the histone h2a(2) type from wheat germ. a core histone type with both, n-terminal and c-terminal extensions. | the histone h2a(2) type from wheat germ comprises at least two highly homologous isohistones with 151 amino acid residues. microheterogeneity occurs mainly at the n-terminal and c-terminal regions. these isohistones have both n-terminal (7 amino acid residues) and c-terminal (15 amino acid residues) extensions relative to calf thymus histone h2a. | 1988 | 3371346 |
| immunization of cattle with a 36-kilodalton surface protein induces protection against homologous and heterologous anaplasma marginale challenge. | immunization of cattle with a purified anaplasma marginale major surface protein, amf36, induced protection against homologous challenge with the florida isolate. similarly, immunized cattle were protected from challenge with the antigenically and structurally distinct washington-o isolate of a. marginale. the degree of protection in amf36-immunized cattle varied from complete prevention of rickettsemia to significant delay in the onset of rickettsemia compared with control immunized cattle. a s ... | 1988 | 3372018 |
| molecular size variations in an immunoprotective protein complex among isolates of anaplasma marginale. | a major surface protein complex from the florida isolate of anaplasma marginale has been previously shown to induce protection in immunized cattle and has been proposed as the basis of a subunit vaccine against anaplasmosis. this complex in the florida isolate is composed of two noncovalently associated polypeptides with molecular masses of 105 and 100 kilodaltons (kda). the analogous protein complex from four geographically different isolates of a. marginale was immunoprecipitated and compared ... | 1988 | 3372021 |
| audiological management in the recovery phase of bacterial meningitis. | during a specified 12-month period, a prospective study of all children admitted to a 139-bed city children's hospital with confirmed bacterial meningitis enabled assessment audiologically at 48 h, 6 weeks and 12 weeks post-admission using a test battery approach including auditory brainstem evoked responses and tympanometry. results suggested conductive dysfunction to be a major cause of fluctuating hearing loss within the group. the incidence of sensori-neural loss was 16.6% of ears tested at ... | 1988 | 3372144 |
| an effective economical intradermal regimen of human diploid cell rabies vaccination for post-exposure treatment. | a closely-spaced multisite intradermal regimen of human diploid cell rabies vaccine (hdcv) was evaluated in 39 patients after low-risk exposure to rabies, in comparison to full-dose intramuscular hdcv and sheep brain-derived rabies (semple) vaccine. the regimen consisted of four intradermal injections, 0.1 ml each of hdcv on days 0, 3 and 7, followed by two booster doses of only 0.1 ml each on days 28 and 91 administered intradermally. although the total amount of hdcv used in this intradermal r ... | 1986 | 3708902 |
| spherocytosis associated with anaplasmosis in two cows. | spherocytes were detected on blood smears of 2 angus cows. the rbc from both cows had increased fragility in hypotonic saline solution, supporting the presence of spherocytes. other laboratory tests revealed a macrocytic, regenerative anemia with anisocytosis, polychromasia, basophilic stippling, howell-jolly bodies, nucleated rbc, and anaplasma marginale organisms. a complement-fixation test was positive for anaplasmosis in cow 1 and a direct coombs' test was positive for immunoglobulin g in bo ... | 1986 | 3710896 |
| gonococcal urethritis with bilateral tysonitis. | a case of gonococcal urethritis with bilateral tysonitis is presented. results of gram's stain and positive culture for neisseria gonorrhoeae confirmed the diagnosis. the patient was treated successfully with aqueous procaine penicillin with probenecid followed by oral ampicillin for ten days. | 1988 | 3391048 |
| the secondary structure of large-subunit rrna divergent domains, a marker for protist evolution. | the secondary structure of the large-subunit ribosomal rna (24-26s rrna) has been studied with emphasis on comparative analysis of the folding patterns of the divergent domains in the available protist sequences, that is prorocentrum micans (dinoflagellate), saccharomyces carlsbergensis (yeast), tetrahymena thermophila (ciliate), physarum polycephalum and dictyostelium discoideum (slime moulds), crithidia fasciculata and giardia lamblia (parasitic flagellates). the folding for the d3, d7a and d1 ... | 1988 | 3395681 |
| biological activities of monoclonal antibodies to the hemagglutinin-neuraminidase (hn) protein of newcastle disease virus. | | 1986 | 3712896 |
| serum zinc in homosexual men with antibodies against human immunodeficiency virus. | | 1988 | 3416468 |
| a microplate enzyme-linked immunosorbent assay for measuring antibody to anaplasma marginale in cattle serum. | an enzyme-linked immunosorbent assay (elisa) method is described for measuring antibody against anaplasma marginale in cattle serum. this method was more sensitive and objective than a previously described elisa method for a. marginale and possible reasons for this are discussed. all 83 cattle experimentally infected with a. marginale (81) or a. centrale (2) developed demonstrable specific antibody but the serums of 98.8% of 839 cattle from cattle tick-free areas did not react by elisa; 378 seru ... | 1986 | 3729834 |
| piperazine resistance in population-b equine strongyles: a study of selection in thoroughbreds in kentucky from 1966 through 1983. | observations were completed over an 18-year period (1966 through 1983) in thoroughbred mares (15/year) and yearlings (11 to 24/year) on a farm where benzimidazole-resistant small strongyles had emerged previously (1962 to 1965). this farm was operated as a closed, nonboarding type, which included a racing stable for its home-bred foals. at 2-week intervals, counts of worm eggs per gram of feces (epg) and larvae per gram of feces were done to monitor strongyle infections and efficacies of bimonth ... | 1988 | 3421545 |
| longevity of colonies of anaplasma marginale in midgut epithelial cells of dermacentor andersoni. | colonies of anaplasma marginale in midgut epithelial cells of experimentally infected dermacentor andersoni were studied in adult ticks 1, 3, and 6 months old. longevity of the parasite in ticks was assessed by evaluating its infectivity for splenectomized calves; calves were exposed by feeding ticks and by inoculation of tick gut homogenates. longevity was also evaluated by determining size, type, and density of colonies in male and female ticks. the effect of incubation (2.5 days at 37 c) on c ... | 1986 | 3752671 |
| percutaneous infection of nymphal dermacentor andersoni with anaplasma marginale. | newly replete nymphal dermacentor andersoni (principals) were percutaneously exposed to anaplasma marginale by injection of either intact or lysed infected bovine erythrocytes. control nymphs were fed on calves with anaplasmosis. the subsequently molted adults were examined for infection by light microscopy, and companion ticks were tested for infectivity by allowing them to feed on susceptible calves. when they fed as adults, both control ticks and percutaneously inoculated principals transmitt ... | 1986 | 3752672 |
| detection of anaplasma-marginale-infected tick vectors by using a cloned dna probe. | anaplasmosis is the most widely distributed of several important tick-borne diseases that constrain cattle production throughout much of the world. evaluation of the effectiveness of disease control strategies that integrate vaccination with tick control requires the ability to monitor tick and cattle infection rates. to detect anaplasma marginale in ticks and bovine erythrocytes, a 2-kilobase dna fragment from a cloned a. marginale gene coding for a surface protein having a mr of 105,000 was pr ... | 1988 | 3422471 |
| influence of castration and testosterone propionate on prostatic and seminal vesicular lipids in mature monkeys. | the effect of castration and administration of testosterone propionate (tp) has been studied in mature monkeys in relation to prostatic and seminal vesicular lipids to castrates on prostatic and seminal vesicular lipids were studied in mature monkeys. castration decreased prostatic total lipid, total phospholipids and total glyceride glycerols. among neutral lipid classes only diacyl glycerol was decreased due to castration. similarly among phospholipid classes, phosphatidyl choline and ethanola ... | 1987 | 3429027 |
| the persistence of colostral anaplasma antibodies and incidence of in utero transmission of anaplasma infections in calves under laboratory conditions. | twenty-six calves, born from 25 anaplasma-infected, intact and splenectomized cows, from a herd kept under strict tick-free laboratory conditions, were monitored for the presence of anaplasma antibodies, using the rapid card agglutination test. serum was collected at birth, weekly for 12 weeks, and then monthly for approximately 6 months. specific antibodies passively acquired could be detected in calf sera for an average period of 8 weeks after birth. calves that remained positive for longer th ... | 1987 | 3444609 |
| sympathetic, muscarinic vasodilation in cranial vessels of the cat. | pressor responses evoked by stimulation of the preganglionic sympathetic trunk of the feline superior cervical ganglion have been recorded in vivo from the vascular bed perfused by one external carotid and the vertebral artery. when vasoconstrictor activity is blocked and potential vasodilator activity enhanced by close, intracarotid injection of guanethidine and prostaglandin f2 alpha respectively, stimulation evokes a weak pressor response followed, on cessation of stimulation, by a prolonged ... | 1986 | 3460813 |
| renal cell carcinoma in the wistar-lewis rat: a model for studying the mechanisms of cholesterol acquisition by a tumor in vivo. | renal adenocarcinoma implanted into isogeneic wistar-lewis rats closely resembles human renal cancer. this paper characterizes the tumor's growth rate, metastatic potential, and its light and electron microscopic appearance. additionally, for the first time, the pathways through which a tumor acquires the cholesterol needed for growth were quantified in vivo. two 1-mg pieces of renal carcinoma were implanted beneath the renal capsule of 80 wistar-lewis rats. of the implanted tumors 95% "took" an ... | 1986 | 3486039 |
| deficient expression of mhc class ii antigens in some cases of human b cell leukaemia. | cells from the peripheral blood of patients with b cell chronic lymphocytic leukaemia (b-cll) and acute lymphoblastic leukaemia (all) were examined for the expression of mhc class ii antigens, using a number of monoclonal antibodies (moab) including l243 (anti-dr) and tu22 (anti-dq). there was wide variation in expression of mhc class ii antigens in cll, both from patient to patient and among cells from the same individual. in a number of subjects a significant proportion of the cells had detect ... | 1986 | 3486060 |
| bovine anaplasmosis: susceptibility of seronegative cows from an infected herd to experimental infection with anaplasma marginale. | adult cows from an anaplasma marginale-infected herd that were negative to the a marginale rapid card agglutination (rca) and complement fixation (cf) tests for 1 to 4 years developed acute anaplasmosis after inoculation with 0.5 ml of blood from an a marginale carrier cow. the test cattle were as susceptible as the control cattle of similar ages. also, 2 cows that had seroconverted from rca/cf-positive to rca/cf-negative status naturally were fully susceptible to anaplasmosis when they were exp ... | 1987 | 3818429 |
| different effects of reinforcing and reducing manipulations in acupuncture assessed by tail-flick latency, vocalization threshold and skin temperature in the rat. | | 1987 | 3497315 |
| isolation and purification of amastigotes of trypanosoma cruzi from cultured vero cells. | a method is described for the isolation and purification of the intracellular amastigotes of trypanosoma cruzi from cultured vero cells. host cells were infected with metacyclic forms obtained in grace's medium. six days after infection, the cells wer subjected to treatment with trypsin to obtain the intracellular forms. the parasites were collected and purified by percoll discontinuous gradient centrifugation. | 1985 | 3885604 |
| kinetic analysis of lamb mutants suggests the signal sequence plays multiple roles in protein export. | we have developed a quantitative assay to measure the rate of processing of precursor lamb into mature protein and have used this assay to characterize 10 previously isolated and 3 new lamb signal sequence mutants. the data suggest that the lamb signal sequence serves a complex function. our assay has revealed five types of signal sequence defect: 1) a strong kinetic defect resulting from alteration of the secondary structure in the putative alpha-helical region in the hydrophobic core, 2) a str ... | 1986 | 3533933 |
| biosynthetic preparation of radioactively labelled ethanolamine plasmalogen (1-o-[1'-14c]octadec-1'-enyl-2-acyl-sn-glycero-3-phosphoethanola mine) using a protozoan cell culture. | ethanolamine plasmalogen radiolabelled mainly in the o-alkenyl moiety was prepared from cell suspension cultures of the flagellate leishmania donovani previously incubated with [1-14c]octadecanol over one growth period. the optimal concentration of [1-14c]octadecanol for labelling was shown to be 1 microm, when 60% of total lipid radioactivity appeared in the 1,2-diradyl-sn-glycero-3-phosphoethanolamine fraction, with an overall yield of approx. 35%. analysis of this fraction revealed that 93% o ... | 1986 | 3536139 |
| high-yield preparation of purified anaplasma marginale from infected bovine red blood cells. | purification of anaplasma marginale from infected bovine rbc was achieved through enzyme treatment and density-gradient centrifugation. a relative yield of 41.6% was obtained by dividing the number of organisms in the final purified preparation by the number of a marginale-infected rbc. purified parasites were verified as a marginale by light microscopy, electron microscopy, and immunologic tests. the purified parasites reacted positively with calf and rabbit anti-a marginale sera in interfacial ... | 1985 | 3898932 |
| transport of 2-deoxy-d-glucose by dissociated brain cells. | the characteristics of glucose transport into dissociated cells from rat brain were determined using [1,2-3h]2-deoxyglucose as substrate. the rate of net uptake exhibited biphasic saturation kinetics with increasing substrate concentration; two values each for km (8.85 and 1.05 mm) and vmax (20.41 +/- 5.99 nmol/min/mg protein) were obtained, indicating the presence of two transport systems. d-glucose competed with [1,2-3h]2-deoxyglucose as shown by increasing degrees of inhibition of uptake of l ... | 1985 | 3899283 |
| neuropeptide y and its flanking peptide in human endocrine tumors and plasma. | neuropeptide y (npy) and its flanking peptide (cpon) were measured in extracts of pheochromocytomas (n = 26), ganglioneuroblastomas (n = 8), and other tumors (n = 95) and plasma (n = 38). npy was present in high concentrations in the majority of the pheochromocytomas, but was absent in 3 tumors. similar concentrations of npy were measured using n- and c-terminal-directed antisera, and there was a good correlation between the concentrations of npy immunoreactivity and cpon immunoreactivity in the ... | 1987 | 3571423 |
| persistence of colonies of anaplasma marginale in overwintering dermacentor variabilis. | dermacentor variabilis were infected as nymphs with anaplasma marginale by allowing the ticks to feed on a single infected donor calf. two weeks after molting to the adult stage, the ticks were allotted into 1 of 3 groups and were allowed to overwinter at room temperature (25 c) in the laboratory (group 1), cold storage (4.5 c) in the laboratory (group 2), or outdoors in leaf litter (group 3). persistence of a marginale was assessed by determining density of colonies (number of colonies/0.1 mm2 ... | 1987 | 3592362 |
| pathology of brain parenchyma in meningeal carcinomatosis; immunohistochemical study with astroprotein (gfap) and tubulin. | effects of leptomeningeal tumor on the brain parenchyma was studied by the immunohistochemical method with astroprotein (gfap) and tubulin in a rat model of meningeal carcinomatosis. thickening of subpial glial lining (external glial layer) and hypertrophy of subpial astrocytes, detected by the antiserum to gfap, was the early sign of parenchymal involvement. the glial lining was continuous as far as the tumor cells were confined to the subarachnoid space, however, penetration of tumor cells int ... | 1987 | 3598623 |
| glutaminyl-trna synthetase of escherichia coli. | | 1985 | 3911010 |
| tick infestations on livestock in the yemen arab republic and their potential as vectors of livestock diseases. | a survey of ticks on cattle, camels, sheep, goats and donkeys in four different geographical locations of the yemen arab republic (yar) was carried out to provide more information on the possible risk of tick-borne diseases to imported exotic cattle included in the yar's plans for livestock development. the most abundant ticks were hyalomma spp. particularly on camels. ticks found on cattle included hyalomma spp., amblyomma variegatum, boophilus annulatus and rhipicephalus spp. in general with t ... | 1987 | 3603710 |
| the oxidant defence system in water-buffaloes (bubalus bubalis) experimentally infected with anaplasma marginale. | the glutathione (gsh) -oxidant defence system protects the erythrocytes and leucocytes from oxidative damage. leucocyte -superoxide dismutase (sod), gsh-peroxidase (gsh-px), gsh-reductase (gr), gsh-s-transferase (gsh-s-t) and arginase were examined in samples from buffaloes infected with anaplasma marginale. all the enzymes, except arginase, were also studied in the red cell haemolysates from these animals. gsh-s-t, gsh- and glutathione-reductase (gr) levels in leucocytes decreased in infected a ... | 1988 | 3369075 |
| withdrawal of zinc oxide-containing diets from mature, female domestic fowl: effects on selected tissue mineral contents. | 1. two hundred and sixteen laying hens of two strains were given diets containing zno to provide 0, 0.5, 0.75, 1.00, 1.25, 1.50, 2.00, 3.00 or 4.00 g added zn/kg. these diets were offered ad libitum for 5 consecutive 28-d periods. all hens were subsequently offered the control diet with no added zno for 5 weeks. 2. four hens from each treatment were killed 0, 1 and 5 weeks after substitution of the zno-supplemented diets by the control diet. 3. dietary addition of zno significantly increased liv ... | 1987 | 3676878 |
| endorectal pull-through with conservation of the rectal mucosa: experimental study. | the present experimental work was designed to study an abdominoanal pull-through technique, with conservation of rectal mucosa. continence was evaluated from clinical, radiologic, and histopathologic standpoints. the animals defecated normally. a good retention of barium was seen in the radiologic enema. on the reexploration of the animals, we found a distal dehiscence with elevation of the intussuscepted stump towards the rectum in all. a reepithelization process of serosa of the intussuscepted ... | 1987 | 3681633 |
| origin of ascending auditory projections to the nucleus mesencephalicus lateralis pars dorsalis in the chicken. | ascending auditory projections to the nucleus mesencephalicus lateralis pars dorsalis (mld) were studied in white leghorn chickens by means of unilateral injections of horseradish peroxidase into the mld and by injections of tritiated leucine into nucleus angularis or the combined nucleus magnocellularis and nucleus laminaris. the experiments showed that nucleus angularis sends an extensive projection to the contralateral mld and a smaller projection to the rostral pole of the ipsilateral mld; t ... | 1986 | 3697720 |
| field observations on adult zebu bulls immunised against anaplasmosis by infection and treatment plus integrated tick control. | fourteen adult zebu bulls which were negative for anaplasma marginale infection both serologically and on blood smear examinations were infected with a virulent nigerian isolate of a. marginale. forty days following the immunising infection when clinical reactions were established groups of seven animals were treated with either two doses of imidocarb dipropionate (2 x 5 mg/kg given intramuscularly 14 days apart) or a single intramuscular dose of long-acting oxytetracycline (5 mg/kg). following ... | 1986 | 3705174 |
| detection of an anaplasma marginale common surface protein present in all stages of infection. | the establishment and maintenance of anaplasmosis-free cattle herds is impaired due to the lack of a rapid, sensitive, and specific serologic test to detect persistently infected cattle which serve as carriers for the organism. to develop an improved diagnostic test for anaplasmosis we screened anaplasma marginale initial body proteins to identify a protein common to antigenically different isolates that is recognized by the host immune system at all stages of infection. seronegative cattle were ... | 1986 | 3711298 |
| epidemiologic aspects of bovine anaplasmosis in semiarid range conditions of south central idaho. | the prevalance of anaplasma marginale-infected cows, as determined by use of the modified rapid card agglutination (mrca) test, was measured during a 4-year period (1980-1983). the prevalence of a marginale-infected cows, defined as positive reactors on the mrca test, remained constant (31%-37%). the apparent incidence of a marginale transmission to susceptible cows was approximately 7% from 1980 to 1981, 8% from 1981 to 1982, and no transmission from 1982 to 1983. the occasional mrca-positive c ... | 1986 | 3963555 |
| complement and hla. further definition of high-risk haplotypes in insulin-dependent diabetes. | the families of 41 probands with type i (insulin-dependent) diabetes mellitus (iddm) were typed for hla-a, hla-b, and hla-dr antigens in addition to the complement polymorphisms c2, c4a, c4b, and bf. all of these loci are encoded on the short arm of human chromosome 6 in a narrow region. alleles at hla-b (8, 15, 18, and 40), hla-dr (3 and 4), and bf (f1) have been associated with increased relative risk (rr) for iddm, while hla-b7 and hla-dr2 have been associated with decreased rr for iddm. this ... | 1985 | 3987976 |
| susceptibility of bos indicus and bos taurus to anaplasma marginale and babesia bigemina infections. | the reaction of bos taurus and pure-bred bos indicus heifers to infection with the intraerythrocytic parasites anaplasma marginale and babesia bigemina was studied. b. bigemina infection at 18 months and a. marginale infection at 13 or 24 months resulted in slightly less severe reactions in pure-bred bos indicus cattle than in bos taurus. in both breeds, the reaction to a. marginale infection was more severe in older cattle. the severity of b. bigemina infection was not affected by a previous in ... | 1985 | 3992874 |
| observations on erythrocytic acetylcholinesterase activity in experimentally induced anaplasmosis in calves. | erythrocytic acetylcholinesterase (ache) activity was assessed in splenectomized bovine calves inoculated with anaplasma marginale. other parameters, i.e., haematological values (haemoglobin, packed-cell volume, and total erythrocytic count), percentage parasitaemia and the ensuing host reactions during anaplasma infection were also studied in relation to ache activity. there was significant inhibition of red blood cell-ache activity commencing soon after inoculation of infected blood; this prec ... | 1985 | 3992879 |
| experimental anaplasmosis in american bison: persistence of infections of anaplasma marginale and non-susceptibility to a. ovis. | blood collected 314 and 496 days after experimentally infecting splenectomized and spleen-intact american bison (bison bison) with anaplasma marginale was infective for splenectomized bovine steers. the pathogenesis was identical to that seen in bovine studies using bovine blood inoculations. a splenectomized bison remained normal clinically, hematologically and serologically for 10 mo after repeated inoculation of ovine blood infected with a. ovis. | 1986 | 3712642 |
| enhanced o6-methylguanine-dna alkyltransferase activity in rat liver nodules. | the progression of chemical carcinogen induced hepatic lesions in the rat from altered enzyme foci to hepatocyte nodules and ultimately to hepatocellular carcinoma appears to be related to the evolution of new cell populations within these hepatic lesions. initiator-promoter-initiator experiments in rat liver models using alkylating agents as the second genotoxic compound suggest accelerated progression toward malignancy could be the result of mutations caused by o6-alkylguanine formation in the ... | 1986 | 3719565 |
| dynamics of anaplasma marginale in splenectomised calves treated with either imidocarb or oxytetracycline. | neither imidocarb dipropionate (at 5 mg/kg body weight intramuscularly once or twice at a seven-day interval) nor oxytetracycline hydrochloride (at 20 mg/kg body weight intramuscularly once or twice at a seven-day interval) given to animals in the prepatent period of anaplasmosis, prevented the eventual onset of infection. both compounds delayed the onset of detectable parasitaemia but this action was more noticeable with oxytetracycline. oxytetracycline given twice significantly reduced the sev ... | 1986 | 3739000 |
| [study of alveolar macrophages stimulated with zymosan in vivo using fluorescent probes]. | zymosan granules injected intravenously (100 mg per kg of body weight) to wistar rats induced strong stimulation of mononuclear phagocyte system. on day 5 zymosan-induced recruitment of monocyte-like cells with high consuming and bactericidal activity into lung spaces could be observed. using fluorescent probes, it was discovered that the size of alveolar cell membranes in zymosan-stimulated or non-stimulated animals was similar, while the viscosity of membrane lipids of alveolar macrophages was ... | 1986 | 3756335 |
| a comparison of the effects of climbing fiber deafferentation in adult and weanling rats. | the climbing fiber input to the cerebellar cortex was destroyed using both electrolytic and chemical (3-acetylpyridine) lesions. the long-term effects of climbing fiber deafferentation on the ansiform lobule of weanling and adult rats were examined at both the light and electron microscopic levels. image analysis of golgi-impregnated purkinje cells indicated a significantly lower number of smooth branches and spiny branchlets following climbing fiber deafferentation of both adult and weanling ra ... | 1986 | 3768690 |
| hexaprenylpyrophosphate synthetase of micrococcus luteus b-p 26. | | 1985 | 4021814 |
| hexaprenylpyrophosphate synthetase of micrococcus luteus b-p 26. | | 1985 | 4021814 |
| vaccination with anaplasma centrale: response after an experimental challenge with anaplasma marginale. | the haematological and clinical responses to vaccination with anaplasma centrale and to subsequent challenge with anaplasma marginale were evaluated. twenty holstein steers 14 to 16 months of age were divided into two groups of 12 and eight animals respectively (groups i and ii). group i was inoculated on day zero with 10(7) a. centrale-infected erythrocytes and group ii was kept as a control. on day 125 both groups were challenged with 5 x 10(7) a. marginale-infected erythrocytes. a. centrale i ... | 1987 | 3629722 |
| [the effect of trapidil and the trapidil derivative ar 12463 on blood pressure and thromboxane formation in spontaneously hypertensive rats]. | the trapidil derivative ar 12463 has a much more stronger inhibiting effect than trapidil on different atherosclerotic parameters of the cell cultures obtained from normal intima and fatty streaks of human aorta, on thromboxane (tx) formation and on the aggregation of human platelets in vitro, and on the activity of phosphodiesterase. in addition to these present results, ar 12463 reduced significantly the mean arterial blood pressure of spontaneously hypertensive rats (at a dosage of 20 mg/kg/d ... | 1987 | 3671447 |
| transmission of anaplasma marginale theiler by males of dermacentor andersoni stiles fed on an idaho field-infected, chronic carrier cow. | the role of ticks and carrier cattle in epizootics of bovine anaplasmosis was further clarified by demonstrating unequivocally, for the first time, that male ticks fed on a chronic carrier cow naturally infected with anaplasma marginale can transmit this parasite intrastadially and biologically when subsequently fed on susceptible cattle. these data indicate that field epizootics of acute anaplasmosis may be initiated by males of tick vector species that feed on carrier cattle and subsequently t ... | 1986 | 3777655 |
| hydatid disease: research and control in turkana. ii. the role of immunological techniques for the diagnosis of hydatid disease. | immunological studies have been applied to the immunodiagnosis of human hydatidosis in turkana and for the specific identification of echinococcus eggs, particularly in regard to their potential for the assessment of a hydatid control programme in the north-west of the district. a high rate of false negatives has been obtained with sensitive antibody tests for proven hydatid patients in turkana and presents problems for prevalence estimation in the population. a seroepidemiological study using s ... | 1986 | 3787677 |
| rem-sleep deprivation-induced increase in ethanol intake: role of brain monoaminergic neurons. | the ethanol intake of long-evans male rats was recorded before, during and after deprivation of rapid eye movement (rem) sleep produced with the flowerpot technique modified by using a cuff pedestal and electrified grid floor instead of water. ethanol intake increased significantly during rem-sleep deprivation. a rebound decrease in ethanol drinking was then observed during the rem-rebound phase immediately after the termination of rem-sleep deprivation. because rem-sleep deprivation has been re ... | 1986 | 3814349 |
| [morphofunctional changes in the adrenergic innervation of the major cerebral arteries during bilateral electrostimulation of the locus coeruleus]. | the intramural adrenergic nervous apparatus of cerebral arteries was studied in adult rabbits after 3-10 sessions of electrical stimulation of locus coeruleus. the activity of nerve structures was determined by estimating the density of adrenergic perivascular plexuses and by semi-quantitative cytophotometry of changes in the catecholamine content of nerve varicosities. the stimulation was followed by a 28.2 +/- 1.5% increase in adrenergic innervation density. while catecholamine content in peri ... | 1987 | 3814808 |
| infectivity of three anaplasma marginale isolates for dermacentor andersoni. | three isolates of anaplasma marginale--virginia (vam), illinois (iam), and florida (fam)--were compared for infectivity for dermacentor andersoni. the isolates were selected, in part, because of a tail-like appendage that has been demonstrated in the vam and iam, but not in the fam. ticks were exposed to the isolates as nymphs either naturally by feeding on a calf with anaplasmosis or artificially by percutaneous inoculation with infected bovine erythrocytes. they were examined for infectivity a ... | 1987 | 3826850 |
| seroepidemiology of bovine anaplasmosis and babesiosis in venezuela. | the serological prevalence of bovine anaplasmosis and babesiosis in the centro-occidental region of venezuela was determined using the indirect fluorescent antibody (ifa) and latex agglutination (lat) tests. anaplasma marginale ifa and lat prevalence rates were 57.7 and 48.6% respectively for cattle of all ages and breeds. the respective prevalence of ifa activity to b. bigemina and b. bovis was 78.2 and 38.8%. the lat test specific only for babesia spp. revealed that 61.4% of the animals tested ... | 1985 | 3887703 |
| development of an indirect fluorescent antibody test, using microfluorometry as a diagnostic test for bovine anaplasmosis. | the indirect fluorescent antibody test for the diagnosis of anaplasma marginale infection in cattle was modified for use with microfluorometry. the test was standardized by use of a fluorometer that measures intensity of fluorescence. standardization included a marginale-infected blood smears on microscope slides as antigen, serum from an inoculated calf as a positive control containing specific antibody, and an affinity-purified fluorescein-conjugated anti-bovine immunoglobulin as 2nd antibody. ... | 1985 | 3890629 |
| in vitro inactivation of tobramycin by cephalosporins. | the in vitro inactivation of tobramycin when combined with each of six cephalosporins in samples of human serum was investigated. each of six cephalosporins (cefazolin sodium, cefoxitin sodium, cefamandole nafate, moxalactam disodium, cefoperazone sodium, and cefotaxime sodium) was added to human serum samples containing tobramycin sulfate 8 micrograms/ml to produce final cephalosporin concentrations of approximately 250 and 1000 micrograms/ml. duplicate solutions were prepared and stored at eit ... | 1985 | 3907338 |
| detection of geographic isolates of anaplasma marginale, using polyclonal bovine antisera and microfluorometry. | geographically separate united states isolates of anaplasma marginale were differentiated, using polyclonal bovine antiserum and microfluorometry. both isolate-common and restricted antigen-specific antibodies were apparent in sera of splenectomized calves after resolution of infection, as judged by the capability of the antisera to recognize heterologous isolate antigens to a lesser extent than homologous antigens. furthermore, absorption of the antisera with homologous antigens removed homolog ... | 1985 | 3907437 |
| modified indirect fluorescent antibody test for the serodiagnosis of anaplasma marginale infections in cattle. | a modified indirect fluorescence antibody technique was used for the serodiagnosis of anaplasma marginale infections in cattle. nonspecific antibodies adherent to infected erythrocytes were removed, using acidic glycine buffer. evans blue was used as a counterstain. | 1985 | 3907438 |
| binding and internalization of heparin by vascular smooth muscle cells. | previous work from our laboratory has demonstrated that heparin specifically inhibits the proliferation of vascular smooth muscle cells in vivo and in vitro. in this paper, we examine the binding and mode of internalization of heparin by smooth muscle cells. for these studies, radiolabeled and fluoresceinated (fitc) heparin probes were synthesized that retained their antiproliferative capacity. binding of 3h-heparin to these cells occurs via specific, high-affinity binding sites (kd = 10(-9) m, ... | 1985 | 3930515 |