| an anaplasma centrale dna probe that differentiates between anaplasma ovis and anaplasma marginale dna. | an anaplasma centrale genomic library was constructed in puc13. two clones pac5 and pac137 hybridising to a. centrale and a. marginale dna were isolated from this library. one of these, pac5, also hybridised to dna from a. ovis. the total insert of pac5 was subcloned into pbr322. this subclone, pac5-12, could detect 1 ng a. centrale, 0.5 ng a. marginale and 3.9 ng a. ovis dna. the hybridisation pattern obtained with pac5-12 on digests of a. centrale, a. marginale and a. ovis dna suggests that th ... | 1991 | 1897134 |
| cloning, sequencing, and expression of the p-protein gene (phea) of pseudomonas stutzeri in escherichia coli: implications for evolutionary relationships in phenylalanine biosynthesis. | the phea gene encoding the bifunctional p-protein (chorismate mutase:prephenate dehydratase) was cloned from pseudomonas stutzeri and sequenced. this is the first gene of phenylalanine biosynthesis to be cloned and sequenced from pseudomonas. the phea gene was expressed in escherichia coli, allowing complementation of an e. coli phea auxotroph. the enzymic and physical properties of the p-protein from a recombinant e. coli auxotroph expressing the phea gene were identical to those of the native ... | 1991 | 1919506 |
| identification of immunodominant polypeptides common between anaplasma centrale and anaplasma marginale. | high titered antibody from rabbits immunized with anaplasma centrale or from cattle recovered from a. centrale infection bound predominantly to several 33-36 kda polypeptides present in both a. centrale and the israel-nt isolate of anaplasma marginale. high titered bovine antibody against the israel-nt isolate of a. marginale also reacted predominantly with a. centrale polypeptides in this size range. the immunodominance of the 33-36 kda polypeptides and their cross-reactivity indicate that thes ... | 1991 | 1949581 |
| 16s ribosomal dna amplification for phylogenetic study. | a set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. one pair of primers is capable of amplifying nearly full-length 16s ribosomal dna (rdna) from many bacterial genera; the additional primers are useful for various exceptional sequences. methods for purification of amplified material, direct sequencing, cloning, sequenci ... | 1991 | 1987160 |
| efficacy of purified anaplasma marginale initial bodies as a vaccine against anaplasmosis. | anaplasma marginale initial bodies of the florida strain were purified from infected erythrocytes using a combination of ultrasonic disruption, nonionic detergent and differential centrifugation. immunochemical analysis revealed at least 12 a. marginale proteins in the molecular mass (m) range 81-15 kda with a prominent band at 38 kda. several of these proteins remained insoluble in the presence of nonionic detergent. preparations of purified anaplasma initial bodies contained negligible erythro ... | 1991 | 2027888 |
| frozen and fresh anaplasma centrale vaccines in the protection of cattle against anaplasma marginale infection. | the immunity induced by frozen and fresh anaplasma centrale vaccines against anaplasmosis caused by a. marginale was tested in 12-month old friesian steers. a. centrale parasitaemia occurred in all cattle inoculated with both types of vaccine. the average maximal decrease in pcv for the frozen and fresh vaccines was 41.0 and 40.3% respectively. all cattle recovered spontaneously. vaccinated and control steers of the same age were challenged six months later with doses of 10(6), 10(7) or 10(8) a. ... | 1990 | 2092348 |
| interaction between parasite and tick vector. | interaction of tick vectors with borrelia species including b. burgdorferi, rickettsias and piroplasms has been demonstrated by describing selected phenomena. in particular, the various environments inside the tick vector have been considered, including the midgut lumen, gut epithelial cells, body cavities and tissues. intracellular parasitism occurs in different compartments of the host cell: parasitophorous vacuoles (anaplasma marginale), phagolysosomes (coxiella spp.), cytoplasm (rickettsia, ... | 1990 | 2120142 |
| immunodiagnosis of and immunoprophylaxis against the haemoparasites babesia sp. and anaplasma sp. in domestic animals. | the haemoparasites babesia bovis and anaplasma marginale are extremely important pathogens of cattle, affecting more than 300 million animals worldwide. species of less importance affect cattle, dogs, horses and sheep. no vaccine is available for widespread usage. this review summarises existing immunodiagnostic and immunoprophylactic methods currently available for these diseases and reports on future immunoprophylactic methods. | 1990 | 2132684 |
| [natural anaplasma marginale infection in 2 cattle herds with different levels of boophilus microplus tick infestation]. | the dynamics of natural infections by anaplasma marginale was studied in two adjacent dairy farms with different levels of boophilus microplus infestation. the farms were located in the enzootic area of bovine anaplasmosis of the northwest of argentina. the study was carried out in 35 calves from birth in march-august 1985 to march 1986. the infection rate by a. marginale was evaluated by the observation of blood films and by determination of specific antibodies. the degree of infestation by b. ... | 1990 | 2132783 |
| in vitro cytotoxic effects of cobalt-containing dusts on mouse peritoneal and rat alveolar macrophages. | "hard metal disease" is a chronic lung disease characterized by the presence of interstitial fibrosis; the role of cobalt in the development of this entity is still debated. indeed, most cases have been observed in some workplaces (grinding) of the hard metal industry and in the diamond polishing industry whereas no similar cases have been reported from workers exposed to pure cobalt powders in cobalt refineries. this study was designed to assess the in vitro toxicity (ldh release, morphology) o ... | 1990 | 2168316 |
| mu-opioid receptors mediate the inhibitory effect of opioids on dopamine-sensitive adenylate cyclase in primary cultures of rat neostriatal neurons. | the receptors mediating the inhibition of d1 dopamine receptor-stimulated adenylate cyclase by opioids were examined in primary cultures of rat neostriatal neurons. adenylate cyclase activity was dose-dependently increased by the selective d1 dopamine receptor agonist skf 38393 (ec50 = 0.05 microm). this stimulation was fully antagonized by the selective d1 dopamine receptor antagonist sch 23390 (1 microm). skf 38393 (1 microm)-stimulated adenylate cyclase activity was strongly reduced (by almos ... | 1990 | 2168934 |
| communication between protein tyrosine and protein serine/threonine phosphorylation. | in the present article, we have reviewed several promising areas of research, the results of which support the existence of enzymatic cascades linking extracellular growth factor receptor/tyrosine kinases with intracellular serine/threonine kinases. proceeding either "upstream" from select serine/threonine kinases or "downstream" from the receptors, characterization of both the distal and proximal elements of these cascades is yielding insights into the multiple pathways of signal transduction. ... | 1990 | 2169797 |
| philippine population development: a nursing concern. | in keeping with nurses' commitment to the preservation and care of human life, population development should be a major concern of the nursing profession in the philippines. the profession should ensure that the health system is oriented toward people's needs and formulate manpower strategies on the basis of these needs and population distribution. nurses are in an ideal position to advocate a family-centered approach to health care through their access to pregnant women and mothers. nurses c ... | 1990 | 2217534 |
| [ticks and hemoparasitic diseases in cattle in senegal. iv. the southern sudan area]. | the authors report on the results of an investigation on ticks and hemoparasitoses of cattle, sheep and goats in the south sudanian area of senegal. systematic routine dipping against ticks of cattle, 40 sheep and 40 goats was set during 15 months, with a view to determine the population dynamics together with an acurate localization of the different species concerned. the following parasites were collected from these ruminants: amblyomma variegatum, boophilus geigyi, hyalomma truncatum, h. m. r ... | 1990 | 2218038 |
| halophilic proteins and the influence of solvent on protein stabilization. | competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment. | 1990 | 2238041 |
| halophilic proteins and the influence of solvent on protein stabilization. | competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment. | 1990 | 2238041 |
| [effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog]. | a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ... | 1990 | 2238764 |
| use of the dot enzyme-linked immunosorbent assay with isolated anaplasma marginale initial bodies for serodiagnosis of anaplasmosis in cattle. | isolated anaplasma marginale initial bodies were successfully used in a dot elisa for rapid detection of antibodies to anaplasma organisms. the enzyme immunoassay used only 25 ng of antigen dotted onto nitrocellulose disks. antigen-antibody complexes were detected by use of alkaline phosphatase-conjugated protein a, and reactions were read visually after addition of a precipitable, chromogenic substrate. the test allowed the processing of multiple sera, either for screening or for titer determin ... | 1990 | 2240769 |
| recombinant dna probe detecting eperythrozoon suis in swine blood. | a genomic library to eperythrozoon suis dna was constructed in lambda gt11, and from this library, e suis clone ksu-2 was identified as a potential diagnostic probe. in hybridization experiments that used 100-microliters samples of blood collected in chaotropic salt solutions, the ksu-2 probe hybridized strongly with purified e suis organisms and blood samples from splenectomized swine that were parasitized with e suis. however, the probe under stringent conditions did not give radiographic indi ... | 1990 | 2240802 |
| an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies to anaplasma centrale and anaplasma marginale. | an enzyme-linked immunoassay (elisa) was applied to detect antibodies to a. centrale and a. marginale using homologous and heterologous antigens. the assay was compared with the indirect fluorescent antibody (ifa) test, and although a similar degree of sensitivity was obtained, the elisa test had several advantages. partially purified anaplasma initial bodies used for antigen preparations contained negligible amounts of residual erythrocytic material, and did not interfere with the specificity o ... | 1990 | 2247936 |
| comparison of a dna probe, complement-fixation and indirect immunofluorescence tests for diagnosing anaplasma marginale in suspected carrier cattle. | most estimates of the prevalence of anaplasmosis have been based on serologic data using the complement-fixation (cf) and/or card agglutination tests. since these tests are considered to be only about 50 percent reliable for detecting carrier cattle in enzootically stable herds, the need for more sensitive diagnostic tests is widely recognized. the objective in the present study was to compare the sensitivity of the cf test with that of the indirect immunofluorescence (iif) test and a recently d ... | 1990 | 2260275 |
| development of a recombinant anaplasma marginale dna probe. | an anaplasma marginale dna probe has been developed by using an improved method for the isolation of genomic dna. purified genomic a. marginale dna from the st. croix isolate was partially digested with sau 3a1 into fragments (greater than or equal to 5.0 kb). the restriction fragments were cloned using standard techniques in the pbr322 vector and used to transform e. coli (dh5) host cells. the recombinant a. marginale dna library was screened by the colony lifting procedure. colonies containing ... | 1990 | 2260276 |
| polyamines in human gastrointestinal malignancies. | this study was designed to investigate the clinical relevance of polyamine determination in human tissue, serum, and urine as diagnostic markers in the diagnosis or postoperative control of patients with gastrointestinal cancer. total, free, and acetylated polyamines were measured simultaneously in tissue, serum, and urine of patients with esophageal (n = 16), gastric (n = 40), pancreatic (n = 20) or colorectal (n = 50) carcinoma, in 40 patients with non-malignant gastrointestinal diseases, and ... | 1990 | 2262073 |
| intermediate site of development of anaplasma marginale in feeding adult dermacentor andersoni ticks that were infected as nymphs. | the development of anaplasma marginale in midgut epithelial cells was studied in feeding, transmitting adult dermacentor andersoni ticks. laboratory-reared ticks experimentally infected as nymphs were allowed to feed from 1 to 9 days on susceptible calves. gut tissues from ticks were collected on each day they fed (total, 9 days) and were processed for light and transmission electron microscopy. colonies of a marginale were abundant during the first 6 days of feeding, after which numbers decreas ... | 1990 | 2301812 |
| cyclic rickettsemia during persistent anaplasma marginale infection of cattle. | submicroscopic levels of anaplasma marginale rickettsemia in persistently infected cattle were determined by using nucleic acid hybridization. within individuals, the rickettsemia levels steadily increased from less than 10(4) infected erythrocytes per ml to a peak of more than 10(6) infected erythrocytes per ml and then rapidly declined. this logarithmic variation parallels the variation of the rickettsemia level seen in acute infection and suggests that cyclic emergence of antigenic variants i ... | 1990 | 2318532 |
| tick-borne diseases of cattle in paraguay. i. seroepidemiological studies on anaplasmosis and babesiosis. | a serological survey was carried out in paraguay to determine the prevalence of antibodies to anaplasma marginale, babesia bigemina and babesia bovis. the results suggest that the parasites are widely distributed throughout the country with prevalence rates of 92% for a. marginale, 79% for b. bigemina and 71% for b. bovis. epidemiological studies on calves demonstrated that initial infection with the three blood parasites occurs in most animals within the first nine months of life. it is likely ... | 1990 | 2321261 |
| plasma and urinary levels of carnitine in different experimental models of hyperammonemia and the effect of sodium benzoate treatment. | the effect of hyperammonemia on plasma and urinary levels of carnitine was studied in different groups of +/y (normal) and spf/y (chronically hyperammonemic) mice. experimental models of acute and subacute hyperammonemia were prepared in +/y and spf/y mice by the use of ammonium acetate ip injections and arginine-free diets, respectively. in acute hyperammonemia, the plasma levels of both free and acylcarnitines increased significantly whereas acyl/free carnitine ratio was decreased, indicating ... | 1990 | 2346672 |
| characterization of a repetitive dna probe for babesia bigemina. | a plasmid (p16) containing a babesia bigemina dna insert was selected and labeled with 32p. this probe was evaluated for specificity and sensitivity by dot blot hybridization. the probe was specific and hybridized with only babesia bigemina dna, and not dna from babesia bovis, bovine leukocyte, trypanosoma brucei or anaplasma marginale. the dna probe detected as little as 10 pg of babesia bigemina dna. the probe hybridized with babesia bigemina isolates from mexico, the caribbean region and keny ... | 1990 | 2382379 |
| development and infectivity of anaplasma marginale in dermacentor andersoni nymphs. | the development of anaplasma marginale was studied in dermacentor andersoni nymphs after they had fed on a calf with ascending anaplasma infection. gut tissues were collected on day 4 of tick feeding, from newly replete (fed) nymphs and on postfeeding days (pfd) 5, 10, 15, 20, and were processed for light and electron microscopy to determine density of a marginale colonies. homogenates of gut tissues were prepared from nymphs collected on the same days and inoculated into susceptible, splenectom ... | 1990 | 2386330 |
| poor blood glucose regulation predicts sleep and memory deficits in normal aged rats. | poor glucose regulation predicts memory deficits in individual elderly humans. the present experiment determined whether glucose regulation was also related to memory and to sleep in aged rodents. glucose regulation, inhibitory avoidance, and daytime sleep were assessed in young (3-month-old) and old (24-month-old) rats. correlations were obtained between glucose regulation and the other variables in individual rats. in old rats, the magnitude of increases in blood glucose levels after glucose i ... | 1990 | 2394910 |
| synthesis and biological activity of guanylhydrazones of 2- and 4-pyridine and 4-quinoline carboxaldehydes. | a series of guanylhydrazones derived from 2- and 4-pyridine and 4-quinoline carboxyaldehydes was synthesized from s-methylisothio-semicarbazide hydroiodide using known procedures. the compounds are analogous to anticancer and antiviral thiosemicarbazones, but several of the guanylhydrazones derived from 4-quinoline carboxaldehyde showed no activity against p388 lymphocytic leukemia in mice. guanylhydrazones derived from all three heterocyclic aldehydes revealed significant blood pressure lowerin ... | 1990 | 2395098 |
| [interaction of local anesthetics with secretory cells at the membrane level]. | | 1985 | 2408715 |
| presence of common antigens, including major surface protein epitopes, between the cattle (intraerythrocytic) and tick stages of anaplasma marginale. | epitopes of major surface proteins of the intraerythrocytic cattle stage of anaplasma marginale were demonstrated in the midgut stage of the organism within the infective tick host dermacentor andersoni. these proteins were common to all a. marginale isolates tested and at all stages of parasitemia. sera from cattle immunized with the tick midgut stage of a. marginale immunoprecipitated multiple-erythrocyte-stage proteins, as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophore ... | 1985 | 2415457 |
| genetic analysis of human b cell hybridomas expressing a cross-reactive idiotype. | we have examined the genetic basis for the expression of a human cross-reactive idiotype (cri) commonly found on monoclonal igm rheumatoid factors. the cri was identified with a monoclonal antibody (17.109) and has been localized previously to the kappa-variable region. by using the human lymphoblastoid cell line wi-l2-729-hf2, and mononuclear cells from several sources, a panel of hybridomas was generated that produced 17.109 cri-positive ig. a recently cloned human germ-line v kappa iii gene, ... | 1987 | 2433336 |
| influence of gangliosides on primary and metastatic neoplastic growth in human and murine cells. | the influence of gangliosides on tumor growth and frequency of metastasis in vivo as well as on growth and motility of neoplastic cells in vitro was tested utilizing human and rodent cell populations. in mice receiving injections of a ganglioside mixture twice daily the tumor volume, the number of spontaneous metastases per animal, and the number of mice with metastasis was approximately double that of controls. preincubation of neoplastic cells with the ganglioside mixture doubled the number of ... | 1987 | 2440560 |
| characterization of an immunoprotective protein complex of anaplasma marginale by cloning and expression of the gene coding for polypeptide am105l. | immunization with an anaplasma marginale surface protein complex containing two polypeptides (am105u and am105l), each having a molecular weight of 105,000, protected cattle against challenge with virulent organisms. these polypeptides were immunoprecipitated together from detergent extracts of a. marginale by a neutralizing monoclonal antibody. after surface radioiodination of intact parasites, both am105u and am105l contained the radiolabel. to define the structural and antigenic relationships ... | 1987 | 2443451 |
| epinephrine impairs insulin release by a mechanism distal to calcium mobilization. similarity to lipoxygenase inhibitors. | the mechanisms that enable epinephrine (epi) and lipoxygenase inhibitors to impede insulin secretion are unknown. we examined the possibility that epi inhibits ca2+ fluxes as its major mechanism by studying 45ca efflux from prelabeled, intact rat islets. epi (2.5 x 10(-7) to 1 x 10(-5) m) inhibited insulin release induced by the influx of extracellular ca2+ (46 mm k+) or the mobilization of intracellular ca2+ stores (2 mm ba2+), but it did not reduce the 45ca efflux stimulated by either agonist. ... | 1988 | 2446939 |
| characterization of a neutralization-sensitive epitope on the am 105 surface protein of anaplasma marginale. | | 1987 | 2448258 |
| differential extraction of antigens of anaplasma marginale. | strain-, group-, and genus-specific antigens of the florida isolate of anaplasma marginale had different solubilities in zwitterionic, nonionic, and anionic detergents. on the basis of their solubility in nonionic detergent, antigens were grouped into 3 classes: (i) a 108-kilodalton (kd) group-specific antigen and a series of poorly defined antigens (70 to 100 kd) were completely soluble in nonionic detergent; (ii) 96 kd and 75 kd group-specific antigens, and a 91-kd strain-specific antigen were ... | 1988 | 2450497 |
| nucleic acid levels in anaplasma marginale during experimentally induced bovine anaplasmosis. | the dna and rna concentrations in uninfected and anaplasma marginale-infected bovine erythrocytes were determined. bovine anaplasmosis was experimentally induced and the nucleic acid levels were followed during the development of the a. marginale infection. two distinct growth stages were found, the "multiplication" stage and the "transfer" stage. based on this hypothetycal developmental cycle, the amount of dna and rna per anaplasma initial body was estimated as 73.1 x 10(-3) pg and 45.7 x 10(- ... | 1988 | 2451913 |
| recognition of conserved surface protein epitopes on anaplasma centrale and anaplasma marginale isolates from israel, kenya and the united states. | | 1988 | 2452797 |
| preliminary studies of the development of anaplasma marginale in salivary glands of adult, feeding dermacentor andersoni ticks. | on each day of feeding on susceptible calves, salivary glands obtained from groups of adult ticks that transmitted anaplasma marginale were examined for a marginale colonies by use of light microscopy and transmission electron microscopy. on day 8 of feeding, salivary glands were examined, using fluorescein-labeled antibody and methyl green-pyronine stain. use of fluorescein-labeled antibody consistently revealed small numbers of fluorescent foci in salivary gland acinar cells obtained from tick ... | 1988 | 2458688 |
| [ticks and hemoparasitoses of livestock in senegal. iii. the northern sudan area]. | the authors describe the results of a study on ticks and hemoparasitoses of cattle and small ruminants in the senegalese north-sudanian area. for 15 months, 40 bovine, 40 sheep and 40 goats received a routine dipping treatment, aimed at the determination of the tick population dynamics together with an accurate localization of the preferential sites for the different species. the following parasites were collected from the animals: hyalomma marginatum rufipes, h. truncatum, rhipicephalus lunulat ... | 1989 | 2485548 |
| [comparative study of the university of wisconsin solution versus eurocollins in kidney transplant]. | uw (university of wisconsin) solution, formulated by belzer's team in madison, has already been proved to increase cold ischemia time in liver and pancreas preservation. a multicentre clinical trial is being conducted to compare renal preservation in human transplantation using two different solutions: uw and eurocollins (ec). this paper, whose results will be included in the multicentre trial, reports local comparative results between uw and ec perfused kidneys. the two donor populations uw (28 ... | 1989 | 2486309 |
| dopamine d1 binding sites in the striatum of the mutant mouse weaver. | in the weaver mouse there is a major abnormality in the dopamine-containing innervation of the striatum. dopamine islands from during development, along with some innervation of the non-islandic matrix; but during the first postnatal month much of the islandic innervation degenerates and there is a failure of the normal postnatal development of the diffuse nigrostriatal innervation. in the experiments reported here we analysed the distribution of d1 dopamine receptor-related binding sites in the ... | 1989 | 2527338 |
| [a new 2-compartment catheter for controlled drainage of hepatic venous blood to the extracorporeal space]. | a new therapeutic catheter designated as "2-compartment-catheter by helzel" for on-target hepatic vein blood drainage to extracorporal space, without blocking the caval blood flow to the right atrium, is presented. its principle of action is outlined. first experiences in animals are reported. using 2 additional catheters, isolated liver perfusion by "pure catheter technique"--without laparotomy--is now possible for various therapeutic approaches. | 1989 | 2538888 |
| [effect of cyclic nucleotides (camp) on experimental hsv-i keratitis]. | the authors report an experimental study on the effect of camp on hsv-i keratitis. rabbit corneas developed typical dendritic keratitis on the 3rd day after inoculation of hsv-i. on the 9th day, the camp level in plasma significantly decreased from 174.9 +/- 20.2 to 52.1 +/- 18.2 pmol/ml, while the cgmp level increased. the camp level in aqueous humour also decreased, with an increase in cgmp level increased. the camp level in aqueous humour also decreased, with an increase in cgmp level. camp w ... | 1989 | 2551611 |
| different gamma-glutamyl transpeptidase mrnas are expressed in human liver and kidney. | in human, the two subunits of gamma-glutamyl transpeptidase (ggt) arise from a common precursor encoded by a multigene family. until now, a single specific coding sequence for this precursor (type i) has been identified in human placenta and liver. in the present study, we have isolated from a human kidney cdna library, a ggt specific clone (0.8 kb). the sequence of which (type ii) i) covers the carboxy terminal part of the ggt precursor, ii) exhibits 22 point mutations and a 30 bp deletion as c ... | 1989 | 2573352 |
| erythrocyte osmotic fragility and cation concentrations during experimentally induced bovine anaplasmosis. | 1. the osmotic fragility, the concentrations of na, k and ca, the osmolality and the total atpase activity of bovine erythrocytes from uninfected and anaplasma marginale-infected bovines were studied in an attempt to correlate these parameters with the decrease in the cellular atp concentration reported during bovine anaplasmosis. 2. the osmotic fragility found in infected bovine erythrocytes, at 0.52% nacl, was about two times greater than that observed in non-infected bovines. the increase in ... | 1989 | 2574096 |
| a simple intravenous infusion method in rodents for determining the potency of anticonvulsants acting through gabaergic mechanisms. | a simple method of intravenous infusion of convulsant drugs (pentetrazol and bicuculline) into the tail vein of rats has been used to determine seizure threshold and construct log-dose seizure threshold response curves for several anticonvulsant drugs (diazepam, phenobarbitone, pentobarbitone, chlormethiazole and valproate). it has been shown that an index of the dose required to increase seizure threshold by 50% (ti50) can be obtained using small numbers of animals. the advantages of this metho ... | 1989 | 2576457 |
| cloned dna probes identify anaplasma ovis in goats and reveal a high prevalence of infection. | anaplasma organisms are observed in erythrocytes from goats with anemia and weight loss in kenya. three anaplasmas have been isolated in nature, anaplasma ovis, anaplasma marginale, and anaplasma centrale. the two recognized species, a. ovis and a. marginale, are known to infect goats. since only a. ovis causes clinical disease in goats, the anaplasma species in goats in kenya were identified. to detect a. ovis, a 9.6-kilobase-pair section of genomic dna was cloned into pbr322 (pao12a) and was u ... | 1989 | 2592538 |
| infectivity and antigenicity of anaplasma marginale from tick cell culture. | the infectivity and immunogenicity of anaplasma marginale grown in a tick cell culture from embryonic dermacentor variabilis ticks were assessed in splenectomized and intact calves, respectively. culture 1 consisted of the cell line inoculated with midguts of adult ticks infected with the mississippi isolate of a marginale and dissected 5 to 10 days after repletion and detachment from an experimentally infected calf. cultures 2 and 3 consisted of the cell line inoculated with midguts of ticks in ... | 1989 | 2610428 |
| delivered volume during high-frequency ventilation. | | 1989 | 2648741 |
| hepatic and biliary tract abnormalities in patients with aids. sonographic-pathologic correlation. | a retrospective evaluation of hepatobiliary sonograms in 22 patients with aids was performed and the sonographic abnormalities were correlated with pathologic findings in 10 patients. hepatic parenchymal abnormalities noted on ultrasound include a hyperechoic parenchymal echo pattern in 45.5%, hepatomegaly in 41%, and focal masses in 9% of patients. etiologies for the diffuse hyperechoic pattern based on pathologic correlation in eight cases were hepatic steatosis and granulomatous hepatitis. bi ... | 1989 | 2654415 |
| neuroendocrinology of the thymus. | the neuropeptides oxytocin (ot) and vasopressin (vp) are synthesized in the human thymus in a similar way as in the hypothalamo-neurophypophyseal system. immunocytochemistry with polyclonal and monoclonal antibodies revealed that immunoreactive ot- and vp-producing cells are localized in the subcapsular cortex and medulla of human and murine thymuses. the epithelial nature of the neuroendocrine thymic cells is demonstrated by their immunostaining with a monoclonal antibody against cytokeratin. a ... | 1989 | 2656471 |
| an early twentieth-century skeleton collection. | the collecting of human remains for study in museums and medical schools has been a vital scientific endeavor for many years. in some cases, such as the fetal and neonatal skeletons discussed, surreptitious dealings were involved. however, one must consider the attitudes that prevailed at the time, the high rate of fetal and infant mortality, and the blossoming role of museums as repositories of knowledge. | 1989 | 2668444 |
| seroepidemiology of anaplasma marginale in white-tailed deer (odocoileus virginianus) from louisiana. | antibodies to anaplasma marginale were detected by the indirect fluorescent antibody test (ifa) in six of 331 (2%) serum samples of white-tailed deer (odocoileus virginianus) from louisiana. none of the serum samples were positive using the a. marginale modified rapid card agglutination test. of the six ifa positive sera retested by the complement fixation test four sera gave anticomplementary and two gave seropositive reactions. the low a. marginale reactor rate in this white-tailed deer popula ... | 1989 | 2668567 |
| seroprevalence of anaplasmosis in the red river plains and south-east areas of louisiana. | the indirect fluorescent antibody test was used to detect antibodies to anaplasma marginale in serum samples collected from cattle in the red river plains and south-east areas of louisiana. convenience sampling of herd clusters was used. a reactor rate of 7.8% was found in 11,085 samples. beef cattle had a 11.2% reactor rate compared with 3.8% for dairy cattle. the angus and angus cross-breed group had the highest reactor rate. | 1989 | 2675458 |
| interrelationships between digestive proteolytic activities and production and quantitation of toxins in pseudomembranous colitis induced by clostridium difficile in gnotobiotic mice. | clostridium difficile pathogenicity is related to in vivo production of toxins, and it is of great interest to detect toxins produced in biological samples. several reports have shown that proteases in stools interfere with immunological methods for quantitation of toxin a. the purpose of this work was to estimate the relationship between the proteases and the c. difficile toxins produced in a gnotobiotic mouse model of pseudomembranous cecitis. cecal proteolytic activities hydrolyzed toxin a, a ... | 1989 | 2680988 |
| anaplasma marginale in tick cell culture. | anaplasma marginale was propagated in a tick cell line derived from dermacentor variabilis embryos. the rickettsial organism was identified and monitored in culture by transmission electron microscopy and the indirect immunofluorescence technique, using specific monoclonal antibodies. inoculation of the embryonic tick cell line with midguts of infected adult ticks (culture 1), nymphal ticks (culture 2) and adult ticks that were infected as nymphs and dissected as adults (culture 3) resulted in 3 ... | 1989 | 2692483 |
| brain biocompatibility of a biodegradable, controlled-release polymer in rats. | we report the biocompatibility in the rat brain of a controlled-release, biodegradable polymer, the polyanhydride poly-[bis(p-carboxyphenoxy)propane-sebacic acid] copolymer (pcpp-sa) in a 20:80 formulation. the biodegradable polyanhydride can be used for drug delivery directly into the brain, circumventing the difficulties posed by the blood-brain barrier and avoiding the consequences of having to administer toxic doses systematically to reach therapeutic doses in the central nervous system. the ... | 1989 | 2708412 |
| levodopa/dopamine analogs as inhibitors of dna synthesis in human melanoma cells. | compounds possessing the ortho-dihydroxybenzene moiety and structurally related to levodopa and dopamine appear to belong to a new class of biologically active agents that are capable of inhibiting dna synthesis in a variety of normal and malignant eukaryotic cells. they appear to work in part through their properties as potent reducing agents, and thereby interfere with several redox reactions that are crucial to the synthesis of cellular dna. interestingly, the compounds can then be converted ... | 1989 | 2715667 |
| antagonism of central and peripheral anorectic effects of caerulein by l-364,718. | the reduction in food intake induced by i.p. injections of the cholecystokinin (cck) analogue caerulein was antagonised by a low dose (0.25 mumol/kg s.c.) of the selective cck antagonist l-364,718. to block the anorectic effect of centrally administered caerulein a dose of 25 mumol/kg of l-364,718 was required, demonstrating that central cck receptors can be blocked effectively in the rat by choosing appropriate doses of l-364,718. | 1989 | 2721558 |
| antagonism of central and peripheral anorectic effects of caerulein by l-364,718. | the reduction in food intake induced by i.p. injections of the cholecystokinin (cck) analogue caerulein was antagonised by a low dose (0.25 mumol/kg s.c.) of the selective cck antagonist l-364,718. to block the anorectic effect of centrally administered caerulein a dose of 25 mumol/kg of l-364,718 was required, demonstrating that central cck receptors can be blocked effectively in the rat by choosing appropriate doses of l-364,718. | 1989 | 2721558 |
| [effect of concomitant pathology and radiation treatment on the immunity indices of patients with laryngeal cancer]. | humoral and cell-mediated immunity in laryngeal cancer patients was evaluated versus presence of concomitant pathology and previous irradiation. the actual t-lymphocyte level in the study group was shown to exceed that measured by reaction of spontaneous rosette formation using sheep erythrocytes. concomitant pathology appeared to impair immune status. distant gamma irradiation suppressed cell-mediated immunity boosting the humoral one. in vitro incubation of peripheral blood lymphocytes with le ... | 1989 | 2741432 |
| [complexing of bovine serum albumin with phosphatidyl choline liposomes]. | thermodynamic parameters of the bovine serum albumin interaction with phosphatidyl choline liposomes have been determined. it is shown that the bovine serum albumin is adsorbed on the liposomal surface without change of the globule conformation, the polar interactions making dominant contribution in immobilization. the complexing is characterized by a binding constant and a great number of binding sites. | 1989 | 2749908 |
| detection of babesia bigemina in cattle by a radioimmunoassay incorporating specifically depleted antigen. | it was observed that mild acidification (ph less than 4.0) together with solvent extraction of the soluble sonicate of a crude preparation of babesia bigemina infected cattle erythrocytes caused a quantitative loss of b. bigemina-specific antigen. cross-reacting antigen activities with babesia bovis remained intact. these properties were utilized in an assay system wherein antibody response to the specifically depleted antigen preparation was subtracted from the response to the initial crude pre ... | 1989 | 2763443 |
| enzymes of oxidant defence system of leucocytes and erythrocytes in bovine anaplasmosis. | the glutathione oxidant defence system in leucocytes and erythrocytes of six anaplasma marginale-infected calves was examined by assaying glutathione peroxidase (gsh-px), glutathione reductase (gssg-r), reduced glutathione (gsh) and superoxide dismutase (sod). in addition, gsh-s-transferase and arginase levels were measured in leucocytes. there was a significant decline in the activities of leucocyte gsh-px, gssg-r and sod in the post-patent period; whereas arginase activity rose significantly f ... | 1989 | 2763451 |
| effects of idebenone on memory impairment induced in ischemic and embolization models of cerebrovascular disturbance in rats. | two rat models of memory impairment in passive avoidance learning induced by cerebrovascular disturbance, were established to estimate the effects of a cerebral metabolic enhancer, idebenone. transient and global cerebral ischemia in rats, produced by 4-vessel occlusion for 200 s immediately after the acquisition trial of passive avoidance learning, shortened the latencies in the retention test trial performed 24 h later. this retrograde amnesia was reversed significantly by idebenone administer ... | 1989 | 2764639 |
| sorbitol and dental plaque. aspects of caries-related microbiological and biochemical factors. | | 1989 | 2772837 |
| transstadial and attempted transovarial transmission of anaplasma marginale by dermacentor variabilis. | transstadial and transovarial transmission of anaplasma marginale by dermacentor variabilis were attempted with with ticks exposed to the organism once by feeding as larvae or nymphs, and twice by feeding as larvae and nymphs. typical colonies of a marginale were in gut tissues of adults that were infected as larvae, larvae and nymphs, and as nymphs; repeated exposure of ticks did not appear to result in an increase in the number of colonies in the gut of subsequently molted adults nor did it af ... | 1989 | 2782719 |
| detection of colonies of anaplasma marginale in salivary glands of three dermacentor spp infected as nymphs or adults. | salivary glands from males of 3 dermacentor species (d andersoni, d variabilis and d occidentalis) that were infected with either the virginia or idaho isolate of anaplasma marginale as nymphs or adults were examined for colonies of a marginale by use of light and electron microscopy. prior to dissection of salivary glands, exposed ticks were held at 25 c for 15 to 18 days, followed by a 3-day incubation at 37 c. ticks of 2 species transmitted a marginale to calves; the third tick species was co ... | 1989 | 2782720 |
| immunization of cattle with the msp-1 surface protein complex induces protection against a structurally variant anaplasma marginale isolate. | the anaplasma marginale surface protein complex msp-1 of the florida isolate is composed of a 105-kilodalton (kda) polypeptide, which bears a neutralization-sensitive epitope, and a 100-kda polypeptide. antigenically similar polypeptides in the okanogan, wash. (washington-o), isolate msp-1 are 86 and 100 kda, respectively. immunization of cattle with florida isolate msp-1 induced antibody titers to both msp-1 polypeptides and protected cattle against homologous and heterologous challenge. | 1989 | 2807542 |
| topically active carbonic anhydrase inhibitors. 1. o-acyl derivatives of 6-hydroxybenzothiazole-2-sulfonamide. | a series of o-acyl derivatives of 6-hydroxybenzothiazole-2-sulfonamide (4, l-643,799) was prepared and the potential utility of each series member as a topically active inhibitor of ocular carbonic anhydrase was determined. in vitro studies showed these esters to be substrates for ocular esterases which liberate 4 during corneal translocation. the most interesting series member, 2-sulfamoyl-6-benzothiazolyl 2,2-dimethylpropionate (22, l-645,151), acting as a prodrug form of 4, was found to enhan ... | 1989 | 2810337 |
| interstitial deletion of (17)(p11.2). a microdeletion syndrome. another example. | another example of del (17)(p11.2) in a 3-year-old boy with psychomotor retardation, broad face, midface hypoplasia, prognathism, and behavioural anomalies was diagnosed clinically and confirmed by prometaphase analysis. it seems that this new microdeletion syndrome may not be so rare. | 1989 | 2817780 |
| in vivo pharmacology of ici 198,615: a novel, potent and selective peptide leukotriene antagonist. | the in vivo pharmacology of ici 198,615, a highly potent and selective peptide leukotriene (lt) receptor antagonist, is described. in a conscious guinea pig model, ici 198,615 provided dose-related antagonism of aerosol ltd4-induced dyspnea when administered p.o., i.v. or by aerosol. the pharmacologic half-lives of ici 198,615 by these routes of administration were greater than 16 hr, 68 min and 34 min, respectively. in pulmonary mechanics studies in which the effects of i.v. or aerosol administ ... | 1987 | 2824752 |
| reinfection of guinea pigs with herpes simplex virus type 2: failure to establish a second latent ganglionic infection. | guinea pigs were infected with herpes simplex virus type 2 (hsv 2) and at various times thereafter reinfected at a different site with the same or a different strain of hsv 2. viruses were isolated from infected tissues either from the site of primary or secondary infection or from regional ganglia. viral isolates were characterised by dna fingerprinting using restriction endonucleases capable of differentiating between the virus strains used. the second infection resulted in a second site of pe ... | 1987 | 2825951 |
| [involvement of arterial vessels in the blood supply to adenomas of the human pituitary, functional implications]. | among 190 human pituitary adenomas investigated, 140 contained arterial blood vessels (nearly 75 percent). in 160 adenomas (prl-, gh-secreting or nonfunctioning tumors) arterial vessels were present in 80 to 90 percent of the cases, whereas in 30 corticotropic adenomas (cushing's disease), 5 only contained arterial vessels. according to data of the literature and to our own findings, such vessels should originate from the anterior pituitary itself or from the surrounding structures (trabecular, ... | 1986 | 2832021 |
| dna probes for the detection of anaplasma centrale and anaplasma marginale. | anaplasmosis can be diagnosed either by immunological techniques or by direct microscopic examination of blood smears. both methods are time-consuming and labour intensive. the use of dna probes in an hybridization assay may simplify the diagnosis of anaplasmosis in cattle and sheep. a genomic dna library of anaplasma centrale was constructed in an expression vector and screened to detect clones containing a. centrale dna. four probes which hybridized to a. centrale and anaplasma marginale dna w ... | 1987 | 2832800 |
| biological activity of 1,25-dihydroxyvitamin d2 and 24-epi-1,25-dihydroxyvitamin d2. | the biological activity of 1,25-dihydroxyvitamin d2 [1,25(oh)2d2] and 24-epi-1,25-dihydroxyvitamin d2 [24-epi-1,25(oh)2d2] has been determined in vitamin d-deficient rats. the biological effectiveness of 1,25(oh)2d2 is equal to that reported previously for 1,25-dihydroxyvitamin d3 [1,25(oh)2d3] (15) in intestinal calcium transport, mineralization of bone, mobilization of bone calcium, and elevation of plasma inorganic phosphorus of rachitic rats. however, 24-epi-1,25(oh)2d2 is at best one-half a ... | 1988 | 2833108 |
| isolation and restriction endonuclease cleavage of anaplasma marginale dna in situ in agarose. | bacterial restriction endonucleases were used to produce dna cleavage patterns that could be useful as tools to study the relatedness among anaplasma marginale isolates. bovine erythrocytes infected with a. marginale were lysed, washed, and embedded in agarose. the embedded erythrocytes and bacterial pathogens were partially digested by sequential infiltration of the agarose with acetone, lysozyme, sodium dodecyl sulfate, and proteinase k. the unfragmented genomic dna was left supported and prot ... | 1988 | 2838504 |
| characterization of a cdna clone encoding the calmodulin-binding domain of mouse brain calcineurin. | a cdna clone corresponding to a portion of the catalytic subunit of calmodulin (cam)-dependent phosphoprotein phosphatase (calcineurin) was isolated from a murine brain library by expression vector immunoscreening. a beta-galactosidase fusion protein that reacted on western blots with anti-calcineurin antibodies and biotinylated cam was purified in preparative amounts using cam-sepharose affinity chromatography. partial digestion of the hybrid protein with staphylococcus aureus v-8 protease prod ... | 1988 | 2848250 |
| [ecto-atpases in leukocytes and its modification by drugs]. | the ecto-atpase is located on the external surface of the plasma membrane of intact cells. the enzyme splits exogeneous atp in the presence of mg2+ into adp and inorganic phosphate. this enzyme has been found on a variety of cells of various species in different activities. in leukocytes sulfhydryl groups are essential for the structure and function of the ecto-atpase. the physiological and biochemical significance of the ecto-atpase for cells remains unclear. drugs may influence ecto-atpase act ... | 1985 | 2859769 |
| high-performance liquid chromatographic assay for dilevalol in human plasma and urine using a prp-1 column and fluorimetric detection. | a single high-performance liquid chromatographic (hplc) assay for the quantitative determination of dilevalol, the r,r isomer of labetalol, was developed for both plasma and urine. a significantly improved limit of detection for dilevalol in plasma was accomplished by extensive modification of an hplc assay originally developed in our laboratory for labetalol. this simplified method is readily adaptable to urine and represents the first reported hplc assay for the quantitative determination of d ... | 1988 | 2897376 |
| dopamine-mediated behaviour following chronic treatment with b-ht 920. | following subchronic (5-day) dosing with b-ht 920 (2-amino-6-allyl-5,6,7,8-tetrahydro-4h-thiazolo(4,5-d)azepine (1 mg kg-1 day-1 i.p.) in rats there was a significant increase in both apomorphine-induced motor activity and stereotypy. on continued b-ht 920 treatment, however, the enhancement of apomorphine motor activity faded into insignificance but the increase in stereotypy persisted beyond 15 days. the results are discussed in terms of dopamine autoreceptor tolerance, postsynaptic d2 superse ... | 1988 | 2905000 |
| alpha adrenoceptor subtypes and receptor reserve in human versus canine saphenous vein: sensitivity to blockade by nitroglycerin. | the purpose of this investigation was to determine the subtypes of alpha adrenoceptors present in human saphenous vein and to determine if there is a large receptor reserve for phenylephrine as has been demonstrated in canine saphenous vein. the subtypes of alpha adrenoceptors found in isolated human saphenous vein were determined using selective alpha-1 and alpha-2 adrenoceptor agonists and antagonists. prazosin, a selective alpha-1 antagonist, produced a parallel shift of the concentration res ... | 1988 | 2905005 |
| detection and quantitation of anaplasma marginale in carrier cattle by using a nucleic acid probe. | cattle which have recovered from acute infection with anaplasma marginale, a rickettsial hemoparasite of cattle, frequently remain persistently infected with a low-level parasitemia and serve as reservoirs for disease transmission. to fully understand the role of these carriers in disease prevalence and transmission, it is essential that low levels of parasitemia can be accurately detected and quantitated. we have developed a nucleic acid probe, derived from a portion of a gene encoding a 105,00 ... | 1989 | 2915021 |
| glucocorticoid receptor activation in isolated perfused rat hearts. | the formation of unactivated and activated glucocorticoid receptor complexes was studied in intact, isolated, perfused rat hearts in the presence of [3h]triamcinolone acetonide. receptor activation, as quantified by the dna-cellulose-binding assay, began to increase within 30 s of perfusion and reached a final steady-state level (t 1/2 = 4.6 min) with 46% of the steroid-receptor complexes bound to dna-cellulose. with the use of a linear potassium phosphate (kp) gradient (5-400 mm), unactivated r ... | 1989 | 2919654 |
| activity of ciprofloxacin against resistant clinical isolates. | the in-vitro activity of ciprofloxacin, a new quinolone derivative, was determined by broth microdilution for 518 clinical isolates. the mics for 90% of the organisms were 2 mg/l for pseudomonas aeruginosa, 8 mg/l for p. maltophilia, 4 mg/l for other pseudomonas spp., 1 mg/l for acinetobacter anitratus, 0.03-0.5 mg/l for differing species of enterobacteriaceae, 2 mg/l for streptococcus faecalis, 0.25 mg/l for coagulase-negative staphylococcus spp. and 2 mg/l for s. aureus. furthermore, on a weig ... | 1986 | 2939048 |
| immunological studies in haemophilic children. | a majority of haemophiliacs who have received large-pool plasma products within the past 5 years have been exposed to the putative agent of the acquired immunodeficiency syndrome (aids)--hiv. it is not known what the risk of infection is among patients in south africa. a study was made of 39 children with congenital coagulation disorders attending the red cross war memorial children's hospital haemophilia clinic. all but 3 had been treated exclusively with small-pool lyophilised cryoprecipitate ... | 1987 | 2953077 |
| [rpo pathway as a possible recombination mechanism of the interaction between dna of transducing phages and the escherichia coli genome]. | the conditions affecting recombination of dnas of transducing lambdoid rifd phages with the chromosome of escherichia coli k-12 reca in the region of homology were studied. in support to the previously obtained data, the int system was shown to take no part in the process. the homologous character of recombination interactions and their dependence on efficiency of transcription were demonstrated. it is therefore suggested that recombination takes the rpo pathway. similar peculiarities were revea ... | 1988 | 2976700 |
| prevalence of cytomegalovirus antibodies in thai blood donors. | antibodies to cytomegalovirus (cmv) were determined in thai blood donors using the complement fixation (cf) test and enzyme-linked immunosorbent assay (elisa). a total of 203 voluntary blood donors, 181 males and 22 females, who came to the blood bank at siriraj hospital during february 1985, were investigated. their ages ranged from 17 to 53 years (mean 24.3 +/- 6.9). seventy-three out of 156 (46.8%) and 171 out of 203 (84.2%) sera were positive for cmv antibodies as detected by the cf test and ... | 1985 | 3000401 |
| bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase. | in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ... | 1986 | 3005034 |
| bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase. | in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ... | 1986 | 3005034 |
| long-term culture of human granulocytes and granulocyte progenitor cells. | | 1986 | 3008194 |
| further evidence that a beta-adrenergic mechanism regulates water intake: role of the subfornical organ. | the daily water intake was reduced in rats by subcutaneous administration of propranolol. the fluid intake after 24 h of dehydration in nephrectomized animals was decreased by propranolol. in intact rats diazepam did not modify the postdehydration water intake. propranolol injected into subfornical organ decreased water consumption after 24 h of fluid deprivation but did not change the dipsogenic response to carbachol given in the same structure. these results suggest that a beta-adrenoceptor me ... | 1986 | 3019711 |
| further evidence that a beta-adrenergic mechanism regulates water intake: role of the subfornical organ. | the daily water intake was reduced in rats by subcutaneous administration of propranolol. the fluid intake after 24 h of dehydration in nephrectomized animals was decreased by propranolol. in intact rats diazepam did not modify the postdehydration water intake. propranolol injected into subfornical organ decreased water consumption after 24 h of fluid deprivation but did not change the dipsogenic response to carbachol given in the same structure. these results suggest that a beta-adrenoceptor me ... | 1986 | 3019711 |
| cloning and characterization of the high-affinity camp phosphodiesterase of saccharomyces cerevisiae. | a gene, pde2, has been cloned from the yeast saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of ras2val19, a mutant form of the ras2 gene that renders yeast cells sensitive to heat shock and starvation. it has previously been shown that the ras proteins are potent activators of yeast adenylate cyclase. we report here that pde2 encodes a high-affinity camp phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. these results t ... | 1986 | 3025832 |
| characterization of mannoproteins from a virulent candida albicans strain and its derived, avirulent strain. | antigenic differences between a wild-type virulent strain of candida albicans 4918 (wt) and its spontaneous avirulent mutant (m-10) were found with crossed-immunoelectrophoresis and western blotting. mannoprotein fractions from yeast and mycelial forms were obtained by affinity chromatography on concanavalin a and analyzed with use of patient sera or sera from rabbits infected with live wt cells and boosted with whole extracts or rabbits immunized with purified wt cell wall preparations. a spike ... | 1988 | 3055210 |
| molecular implications of ph (+) myelodysplastic syndrome. | we report a case of 62-year-old japanese male with a myelodysplastic syndrome (mds) with a philadelphia (ph) chromosome. cytogenetic analysis revealed the bone marrow cells to contain a ph chromosome due to t(?;11;22) (?;q11;q11), as well as -5, -7, +8, -12 and an extra ph, in addition to cells with a normal karyotype. molecular analysis using breakpoint cluster region probes (5' bcr and 3' bcr) did not detect a rearrangement within the bcr dna sequences, indicating that the breakpoint at 22q11 ... | 1988 | 3066168 |