| molecular identification of tick-borne pathogens in nigerian ticks. | a molecular epidemiology investigation was undertaken in two nigerian states (plateau and nassarawa) to determine the prevalence of pathogens of veterinary and public health importance associated with ticks collected from cattle and dogs using pcr, cloning and sequencing or reverse line blot techniques. a total of 218 tick samples, amblyomma variegatum (n=153), rhipicephalus (boophilus) decoloratus (n=45), and rhipicephalus sanguineus (n=20) were sampled. pathogens identified in ticks included p ... | 2012 | 22326937 |
| molecular detection of anaplasma marginale infection in carrier cattle. | anaplasma marginale infections are responsible for widespread morbidity and mortality particularly in crossbred and exotic breeds of cattle in the tropics and subtropics. in the present study, a semi-nested pcr assay was utilized for the detection of a. marginale infection in carrier cattle from different areas of punjab state, india. an amplicon of 458 bp specific for msp5 of a. marginale was detected in 45.2% of blood samples when subjected to primary pcr assay against the routine blood smear ... | 2012 | 22309860 |
| prevalence of livestock diseases and their impact on livelihoods in central equatoria state, southern sudan. | a participatory epidemiological (pe) study was conducted in kajo keji and yei counties, central equatoria state, southern sudan to assess the impact of livestock diseases on livelihoods. a serological survey of tick-borne diseases was conducted to supplement the pe study. pe data collection tools consisted primarily of focus group interviews and key informant interviews supplemented by observation. information was collected on the social context, history and species of livestock kept. constraint ... | 2012 | 22244519 |
| comparison of microscopy and pcr-rflp for detection of anaplasma marginale in carrier cattle. | in iran, anaplasmosis is normally diagnosed with traditional giemsa staining method. this is not applicable for identification of the carrier animals. the aim of this study was to compare the detection of anaplasma marginale in two different numbers of microscopic fields (50 and 100) using conventional giemsa staining method compared with the pcr-rflp technique. | 2010 | 22347555 |
| characterization of recombinant msp5 anaplasma marginale havana isolate. | anaplasma marginale is the causative agent of bovine anaplasmosis, a disease of worldwide economic importance. major surface proteins (msps) are involved in host-pathogen and tick-pathogen interactions and they have been used as markers for the genetic characterization of a. marginale strains and phylogenetic studies. the major surface protein 5 (msp5) is highly conserved in the genus anaplasma and in all isolates of a. marginale. the aim of the present work was to carry out the cloning, sequenc ... | 2009 | 24031449 |
| morphology of colonies of anaplasma marginale in nymphal dermacentor andersoni. | colonies of anaplasma marginale theiler were studied in midgut epithelial cells of nymphal dermacentor andersoni stiles that had become infected by feeding on splenectomized calves with anaplasmosis. colonies of a marginale were not observed in nymphal ticks killed during the 6-day feeding period, but were present in sections of midgut epithelial cells of ticks killed as early as 5 days after repletion. colonies of a marginale also were present in ticks examined throughout development to the adu ... | 1984 | 24049913 |
| molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france. | corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ... | 2017 | 28645313 |
| assessment of a quantitative 5' nuclease real-time polymerase chain reaction using groel gene for ehrlichia and anaplasma species in rodents in brazil. | new genotypes of anaplasmataceae agents have been detected in wild carnivores, birds and deer in brazil. the present work aimed to investigate the presence of ehrlichia and anaplasma species in rodents sampled in brazil. additionally, a newly designed quantitative 5' nuclease real-time multiplex pcr for ehrlichia and anaplasma spp. detection based on groel gene amplification was designed, showing high specificity and sensitivity (10 groel fragment copy/μl). between 2000 and 2011, different roden ... | 2017 | 28457822 |
| molecular epidemiological survey and genetic characterization of anaplasma species in mongolian livestock. | anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. few studies on anaplasma infections in mongolian livestock have been conducted. this study examined the prevalence of anaplasma marginale, anaplasma ovis, anaplasma phagocytophilum, and anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (bos taurus), yaks (bos grunniens), sheep (ovis aries), and goats (capra a ... | 2017 | 28678004 |
| seasonal prevalence of blood parasitic diseases in crossbred cattle of mysore and its surrounding districts of karnataka. | the present study report the seasonal prevalence of blood parasitic diseases in cross bred cattle in mysore and its surrounding districts of karnataka such as mandya, chamaraj nagar and kodagu. the study was undertaken for a period of 2 years from august 2013 to july 2015. a total of 1655 blood samples were collected from clinically suspected cattle for blood parasitic diseases with clinical symptoms of anorexia, high fever, anaemia, salivation, enlargement of superficial lymphnodes, haemoglobin ... | 2017 | 28848277 |
| rhipicephalus microplus and dermacentor nitens (acari: ixodidae) coparasitize white-tailed deer on st. john, u.s. virgin islands. | ticks parasitizing introduced white-tailed deer, odocoileus virginianus (zimmermann), on st. john, u.s. virgin islands, were recorded during and after drought conditions. tick infestation prevalences were 22% at the start of the drought (july 2015), 66% at the height of the drought (march 2016), and 35% after the drought had ended (july 2016; n = 67 deer). samples of ticks from 22 tranquilized deer in july 2016 revealed the presence of two species, the southern cattle tick, rhipicephalus (boophi ... | 2017 | 28591859 |
| a nano particle vector comprised of poly lactic-co-glycolic acid and monophosphoryl lipid a and recombinant mycobacterium avium subsp paratuberculosis peptides stimulate a pro-immune profile in bovine macrophages. | we evaluated the potential of a nanoparticle (np) delivery system to improve methods of delivery of candidate peptide-based vaccines for paratuberculosis in cattle. | 2017 | 28502107 |
| validation of an improved anaplasma antibody competitive elisa for detection of anaplasma ovis antibody in domestic sheep. | an accurate and simple-to-perform new version of a competitive elisa (celisa) kit that became commercially available in 2015 for testing of cattle for antibody to anaplasma marginale was validated for detection of anaplasma ovis antibody in domestic sheep. true positives and negatives were identified using nested pcr (npcr) as the gold standard. negative bovine control sera supplied with the kit were used to calculate % inhibition (%i), designated bovine control elisa (bcelisa), and this was com ... | 2017 | 28480803 |
| the transcription factor relish controls anaplasma marginale infection in the bovine tick rhipicephalus microplus. | rhipicephalus microplus is an important biological vector of anaplasma marginale, the etiological agent of bovine anaplasmosis. the knowledge of tick immune responses to control bacterial infections remains limited. in this study, we demonstrate that transcription factor relish from the imd signaling pathway has an important role in the control of a. marginale infection in ticks. we found that rna-mediated silencing of relish caused a significant increase in the number of a. marginale in the mid ... | 2017 | 28408334 |